| 1. |
Yuji Atsuta, Direct reprogramming of non-limb fibroblasts to cells with properties of limb progenitors, 第５５回日本発生生物学会年会, 2022.06, The limb bud progenitors (LPs), which originate from the somatopleural layer of the lateral plate mesoderm, give rise to the majority of tissues within the mature patterned limb including bones, cartilage and tendons. The lateral plate mesoderm also gives rise to the mesodermal components of the trunk, flank and neck. However, the mesenchymal cells generated at these other axial levels cannot produce the variety of cell types found in the limb, nor can they be directed to form a patterned appendage-like structure, even when placed in the context of the signals responsible for organizing the limb bud. To identify factors involved in establishing limb progenitor properties in lateral plate mesoderm, we took a reprogramming approach, reasoning that the full set of the factors specifying “limbness” might be sufficient to convert non-limb fibroblasts into LP-like cells. As a result, we find that a set of factors (Prdm16, Zbtb16 and Lin28a) is capable of imparting LP-like properties to non-limb fibroblasts. Cells reprogrammed by these factors show similar gene expression profiles, and can differentiate into similar cell types, as endogenous LPs. The further addition of Lin41 potentiates proliferation of the reprogrammed cells while suppressing differentiation. These results suggest that these same four key factors may play pivotal roles in the specification of legitimate LPs.. |
| 2. |
Yuji Atsuta, Making vertebrate limbs from non-limb fibroblasts, SDB 79th Meeting, 2020.07. |
| 3. |
Atsuta Y. , Reprogramming non-limb fibroblasts into limb progenitor-like cells
, NAIST Bioscience Seminar, 2018.11. |
| 4. |
Atsuta Y. and Takahashi Y. , Tubulogenesis using Wolffian duct as a model: Tubule elongation and cell epithelialization are coordinated by FGF signals.
, Japanese Society of Developmental Biologists (JSDB) 47th Annual Meeting, 2014.05. |
| 5. |
Atsuta Y., Ueda S. and Takahashi Y. , Tubule elongation and cell epithelialization are regulated by FGF signals emanating from adjacent tissues
, NAIST International Symposium “Top Runners～Women’s Life in Science, 2012.01. |
| 6. |
Atsuta Y. and Tabin C. , Reprogramming non-limb fibroblasts into limb bud progenitor-like cells
, EMBO workshop. Limb Development and Regeneration: New Tools for a Classic Model System, 2019.07. |
| 7. |
Atsuta Y., Tomizawa R., Levin M. and Tabin C. , Assessing the roles of bioelectric signaling in embryonic patterning using vertebrate limb buds
, JSDB 52nd Annual Meeting, 2019.05. |
| 8. |
Atsuta Y., Lee C., Rodrigues A. and Tabin C. , Reprogramming non-limb fibroblasts into limb bud progenitor-like cells
, 第41回日本分子生物学会年会, 2018.11. |
| 9. |
Atsuta Y., Rodrigues A., Lee C., Colle C. and Tabin C. , Reprogramming non-limb fibroblasts into limb bud progenitor-like cells
, Society for Developmental Biologists (SDB) 77th Annual Meeting, 2018.07. |
| 10. |
Atsuta Y. Levin M. and Tabin C. , Assessing the roles of bioelectric signaling in embryonic patterning using the chicken limb bud as a model
, SDB 76th Annual Meeting, 2017.07. |
| 11. |
Atsuta Y., Colle C. and Tabin C., Designing novel culture systems for long-term expansion of limb bud progenitors
, Joint Meeting of the German and JSDB, 2017.03. |
| 12. |
Atsuta Y. and Takahashi Y. , Tubulogenesis using Wolffian duct as a model: Tubule elongation and cell epithelialization are coordinated by FGF signals
, SDB 73th Annual Meeting, 2014.07. |
| 13. |
Atsuta Y. and Takahashi Y. , Coordination between tubular elongation and cell epithelialization is regulated by FGFs emanating from surrounding tissues
, JSDB 46th Annual Meeting, 2013.05. |
| 14. |
Atsuta Y., Ueda S. and Takahashi Y. , Tubule elongation and cell epithelialization are coordinately regulated by FGFs emanating from adjacent tissues
, JSDB 45th ＆ JSCB 64th Joint Meeting, 2012.05. |
| 15. |
Atsuta Y. and Takahashi Y. , Tubulogenesis using Wolffian duct as a model: FGF signals regulate tubular elongation and cell epithelialization as environmental factors
, JSDB 44th Annual Meeting, 2011.05. |
| 16. |
Atsuta Y. and Takahashi Y. , Tubular extension and cell epithelialization are coordinately regulated and influenced by adjacent tissues
, The 16th International Conference of ISD, 2010.10. |
| 17. |
Atsuta Y., Ohata E., Tadokoro R., Saito D. and Takahashi Y. , Tubular extension and cell epithelialization are coordinately regulated and influenced by adjacent tissues
, SDB 69th annual meeting, 2010.08. |
| 18. |
Atsuta Y., Ohata E., Tadokoro R., Saito D. and Takahashi Y., Epithelialization and extension of tubular structures are regulated by interactions between neighboring tissues
, JSDB 43rd Annual Meeting, 2010.06. |
| 19. |
Atsuta Y., Ohata E., Tadokoro R., Saito D. and Takahashi Y. , Tubular formation using Wolffian duct as a model: Tubular extension and cell epithelialization are coordinately regulated.
, CDB Symposium “Frontiers in Organogenesis”, 2010.03. |
| 20. |
Atsuta Y., Ohata E., Tadokoro R., Saito D. and Takahashi Y. , Tubular formation using Wolffian duct as a model: Tubular extension and cell epithelialization are coordinately regulated
, 第32回日本分子生物学会年会, 2009.12. |
| 21. |
Atsuta Y., Tadokoro R. and Takahashi Y., In vivo time-lapse imaging of individual cells in an actively extending kidney rudiment, JSDB 42nd Annual Meeting, 2009.05. |
