Kyushu University Academic Staff Educational and Research Activities Database
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Kenta Shigetomi Last modified date:2023.04.26

 Reseacher Profiling Tool Kyushu University Pure
Academic Degree
Field of Specialization
cell biology
Research Interests
  • mechanisms of tight junction turn over
    keyword : Claudin, turn over, degradation
  • Functional analysis of membrane lipids in tight junction formation
    keyword : Cell adhesion, Tight junction, Membrane lipid, Claudin
Academic Activities
1. @Kenta Shigetomi, Yumiko Ono, Kenji Matsuzawa, Junichi Ikenouchi, Cholesterol-rich domain formation mediated by ZO proteins is essential for tight junction formation, Proceedings of the National Academy of Sciences,, 2023.02.
2. Kenta Shigetomi, Yumiko Ono, Tetsuichiro Inai, Junichi Ikenouchi, Adherens junctions influence tight junction formation via changes in membrane lipid composition, Journal of Cell Biology, 10.1083/jcb.201711042, 217, 7, 2373-2381, 2018.07, Tight junctions (TJs) are essential cell adhesion structures that act as a barrier to separate the internal milieu from the external environment in multicellular organisms. Although their major constituents have been identified, it is unknown how the formation of TJs is regulated. TJ formation depends on the preceding formation of adherens junctions (AJs) in epithelial cells; however, the underlying mechanism remains to be elucidated. In this study, loss of AJs in α-catenin-knockout (KO) EpH4 epithelial cells altered the lipid composition of the plasma membrane (PM) and led to endocytosis of claudins, a major component of TJs. Sphingomyelin with long-chain fatty acids and cholesterol were enriched in the TJ-containing PM fraction. Depletion of cholesterol abolished the formation of TJs. Conversely, addition of cholesterol restored TJ formation in α-catenin-KO cells. Collectively, we propose that AJs mediate the formation of TJs by increasing the level of cholesterol in the PM..