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Ishii Chiharu Last modified date：2024.06.03

Assistant Professor / Department of Chemo-Pharmaceutical Sciences
Faculty of Pharmaceutical Sciences


Graduate School
Department of Medicinal Sciences
Graduate School of Pharmaceutical Sciences
Undergraduate School
Department of General Pharmaceutical Sciences
School of Pharmaceutical Sciences


E-Mail　*Since the e-mail address is not displayed in Internet Explorer, please use another web browser:Google Chrome, safari.
Homepage
https://kyushu-u.elsevierpure.com/en/persons/chiharu-ishii
　Reseacher Profiling Tool　Kyushu University Pure
Phone
092-642-6598
Fax
092-642-6598
Academic Degree
Doctor of Philosophy (Medicinal Sciences)
Country of degree conferring institution (Overseas)
No
Field of Specialization
Analytical chemistry
Total Priod of education and research career in the foreign country
00years05months
Research
Research Interests
  • Development of a two-dimensional LC-MS/MS system for the precise determination of intrinsic chiral amino acids and screening of biomarkers for diseases
    keyword : Free D-amino acids, Multidimensional HPLC, MS/MS, Biomarker
    2021.03.
  • Screening of new diagnostic markers and therapeutic targets of age-related diseases focusing on isomerization of amino acid residues in proteins
    keyword : Protein, Amino acid residues, Isomerization, Multi-dimensional HPLC, MS/MS
    2021.03.
  • Clarification of regulation mechanisms of intrinsic D-amino acid amounts in mammals
    keyword : Intrinsic D-amino acids, Regulation, Racemase, Oxidase
    2021.03.
  • Screening of new functional food/beverages naturally containing D-amino acids
    keyword : D-Amino acids, Functional food
    2021.03.
Academic Activities
Books
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1. Chiharu Ishii, Reiko Koga, Kenichiro Todoroki, Kenji Hamase, 3rd Edition of the Handbooks in Separation Science: Liquid Chromatography, Applications, Vol II with Chapter 4 on Amino acid and bioamine separations, Elsevier, 2023.04.
Reports
 Show All Reports >>
1. Chiharu ISHII, Kenji HAMASE, Development of Two-Dimensional LC-MS/MS Methods for Highly-Selective Analysis of Chiral Amino Acids and the Evaluation of Their Intrinsic Amounts in Mammals and Stereoinversion in Proteins, Chromatography, 10.15583/jpchrom.2024.001, 2024.02.
2. Chiharu ISHII, Kenji HAMASE, Two-dimensional LC-MS/MS and three-dimensional LC analysis of chiral amino acids and related compounds in real-world matrices, Journal of Pharmaceutical and Biomedical Analysis, 10.1016/j.jpba.2023.115627 , 2023.08.
3. Chiharu ISHII, Aogu FURUSHO, Chin-Ling HSIEH, Kenji HAMASE, Multi-dimensional high-performance liquid chromatographic determination of chiral amino acids and related compounds in real world samples, Chromatography, 10.15583/jpchrom.2020.004, 2020.02.
Papers
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1. Chiharu ISHII, Yosuke TOJO, Komei IWASAKI, Akira FUJII, Takeyuki AKITA, Masanobu NAGANO, Masashi MITA, Tomomi IDE, Kenji HAMASE, Development of a two-dimensional LC-MS/MS system for the determination of proline and 4-hydroxyproline enantiomers in biological and food samples, Analytical Sciences, 10.1007/s44211-024-00530-w, 40, 881-889, 2024.04.
2. Chiharu ISHII, Takeyuki AKITA, Tomonori KIMURA, Shinsuke SAKAI, Masashi MITA, Tomomi IDE, Yoshitaka ISAKA, Kenji HAMASE, Evaluation of individual variation of D-amino acids in human plasma by a two-dimensional LC-MS/MS system and application to the early diagnosis of chronic kidney disease, Analytical Chemistry, 10.1021/acs.analchem.3c05309, 96, 4876-4883, 2024.03, For the discovery of sensitive biomarkers of kidney function focusing on chiral amino acids, a multiple heart-cutting two-dimensional (2D) liquid chromatography−mass spectrometry (LC−MS)/MS system has been designed/developed. As the target analytes, alanine (Ala), aspartic acid, glutamic acid (Glu), leucine (Leu), lysine, methionine, phenylalanine (Phe), proline (Pro), serine (Ser), and valine were selected considering the presence of their D-forms in mammals. The 2D LC−MS/MS system consisted
of the nonenantioselective reversed-phase separation of the target amino acids, the separations of the D- and L-enantiomers, and detection using MS/MS. Using the method, the plasma chiral amino acids, precolumn derivatized with 4-fluoro-7-nitro-2,1,3-benzoxadiazole, were isolated from other intrinsic substances, then determined without losing sensitivity by the fully automated whole-peak volume transfer operation from first to second dimension. In all of the tested plasma samples obtained from five healthy individuals and 15 patients with chronic kidney disease (CKD), the target chiral amino acids were determined without interference. In healthy individuals, the levels of all the tested D-amino acids were regulated in the low ranges. In contrast, the % D values of Glu, Leu, and Phe significantly increased with the progress of kidney dysfunction, besides the previously reported values of D-Ala, Pro, and Ser. Concerning Phe, the significant increase of the % D values (p
3. C. Ishii, T. Akita, M. Mita, R. Konno, K. Hamase, Chiral amino acid analysis in the plasma of B6DAO-/- mice lacking D-amino acid oxidase activity, Chromatography, 10.15583/jpchrom.2023.002, 44, 39-43, 2023.02.
4. Chiharu Ishii, Noburo Takizawa, Takeyuki Akita, Masashi Mita, Tomomi Ide, Ryuichi Konno, Kenji Hamase, Off-line two-dimensional LC-MS/MS determination of tryptophan enantiomers in mammalian urine and alteration of their amounts in D-amino acid oxidase deficient mice, Journal of Pharmaceutical and Biomedical Analysis, 10.1016/j.jpba.2022.114919, 219, 114919, 2022.06.
5. Yukiko Shimizu, Chiharu Ishii, Rieko Yanobu-Takanashi, Kenta Nakano, Akio Imaike, Masashi Mita, Kenji Hamase, Tadashi Okamura, D-Amino acid oxidase deficiency is caused by a large deletion in the Dao gene in LEA rats, Biochim. Biophys. Acta-Proteins and Proteomics, 10.1016/j.bbapap.2020.140463, 2020.06.
6. Chiharu ISHII, Takeyuki AKITA, Masanobu NAGANO, Masashi MITA, Kenji HAMASE, Determination of chiral amino acids in various fermented products using a two-dimensional HPLC-MS/MS system, Chromatography, 10.15583/jpchrom.2019.011, 2019.03.
7. Chiharu ISHII, Takeyuki AKITA, Masashi MITA, Tomomi IDE, Kenji HAMASE, Development of an online two-dimensional high-performance liquid chromatographic system in combination with tandem mass spectrometric detection for enantiomeric analysis of free amino acids in human physiological fluid, Journal of Chromatography A, 10.1016/j.chroma.2018.07.076, 1570, 91-98, 2018.07.
8. Chiharu ISHII, Tetsuya MIYAMOTO, Shoto ISHIGO, Yurika MIYOSHI, Masashi MITA, Hiroshi HOMMA, Tadashi UEDA, Kenji HAMASE, Two-dimensional HPLC-MS/MS determination of multiple D-amino acid residues in the proteins stored under various pH conditions, Chromatography, 10.15583/jpchrom.2017.009, 38, 65-72, 2017.05.
Presentations
 Show All Presentations >>
1. Chiharu ISHII, Takeyuki AKITA, Masashi MITA, Tomomi IDE, Tomonori Kimura, Kenji HAMASE, Selective determination of D-amino acids in human plasma using a two-dimensional LC-MS/MS system toward early-diagnosis of chronic kidney disease, 33rd International Symposium on Pharmaceutical and Biomedical Analysis, 2023.07.
2. Chiharu ISHII, Takeyuki AKITA, Masashi MITA, Tomomi IDE, Tomonori KIMURA, Kenji HAMASE, Screening of new diagnostic markers for chronic kidney disease focusing on chiral amino acids using a highly selective two-dimensional LC-MS/MS system, 51st International Symposium on High Performance Liquid Phase Separations and Related Techniques, 2023.06.
3. Chiharu Ishii, Yukiko Shimizu, Takeyuki Akita, Masashi Mita, Tadashi Okamura, Kenji Hamase, Selective determination of D-amino acids in the rat plasma and urine using a two-dimensional LC-MS/MS system and their regulation by D-amino acid oxidase, 12th International symposium on drug analysis and 32nd International symposium on pharmaceutical and biomedical analysis, 2022.09.
4. Chiharu Ishii, Yukiko Shimizu, Takeyuki Akita, Masashi Mita, Tadashi Okamura, Kenji Hamase, Simultaneous determination of chiral amino acids in the plasma and brain of DAO deficient rats using a highly selective two-dimensional LC-MS/MS system, The 5th international conference of D-amino acid research, 2022.07.
5. Chiharu ISHII, Yukiko SHIMIZU, Takeyuki AKITA, Masashi MITA, Tomomi IDE, Tadashi OKAMURA, Kenji HAMASE , Determination of plasma D-amino acids in human and laboratory rodents with various D-amino acid oxidase activities using a two-dimensional HPLC-MS/MS system, Pacifichem2021, 2021.12.
6. Chiharu ISHII, Yukiko SHIMIZU, Takeyuki AKITA, Masashi MITA, Tomomi IDE, Tadashi OKAMURA, Kenji HAMASE, Development of a two-dimensional LC-MS/MS system for the selective determination of intrinsic chiral amino acids and application to mammalian plasma, 31st International Symposium on Pharmaceutical and Biomedical Analysis, 2021.08.
7. Chiharu ISHII, Takeyuki AKITA, Masashi MITA, Tomomi IDE, Kenji HAMASE, Two-Dimensional HPLC-MS/MS Determination of Intrinsic Chiral Amino Acids in the Plasma of Mammals with Various D-Amino Acid Oxidase Activities, 49th International Symposium on High Performance Liquid Phase Separations and Related Techniques, 2019.12.
8. Chiharu ISHII, Takeyuki AKITA, Masashi MITA, Tomomi IDE, Kenji HAMASE, Development of a highly selective two-dimensional HPLC-MS/MS system for the screening of intrinsic D-amino acids in human clinical samples, 30th International Symposium on Pharmaceutical and Biomedical Analysis, 2019.09.
9. Chiharu ISHII, Takeyuki AKITA, Masashi MITA, Kenji HAMASE, Determination of D-amino acids in fermented products using an automated two-dimensional HPLC-MS/MS system, 35th International Annual Meeting in Pharmaceutical Sciences, 2019.03.
10. Chiharu ISHII, Takeyuki AKITA, Masashi MITA, Tomomi IDE, Kenji HAMASE, Development and validation of an on-line two-dimensional HPLC-MS/MS system for the determination of intrinsic chiral amino acids in human physiological fluids, 29th International Symposium on Pharmaceutical and Biomedical Analysis, 2018.09.
11. Chiharu ISHII, Takeyuki AKITA, Masashi MITA, Tomomi IDE, Kenji HAMASE, Selective screening of free D-amino acids in the physiological fluids and D-form residues in the proteins using an automated two-dimensional HPLC-MS/MS system, 2018 Sino-Japanese joint symposium on separation sciences, 2018.07.
12. Chiharu ISHII, Masashi MITA, Takeyuki AKITA, Tomomi IDE, Kenji HAMASE, Development of a fully automated two-dimensional HPLC-MS/MS system for the determination of amino acid enantiomers in human physiological fluids and fermented beverages, 34th International Annual Meeting in Pharmaceutical Sciences, 2018.03.
13. Chiharu ISHII, Tetsuya MIYAMOTO, Shoto ISHIGO, Masashi MITA, Hiroshi HOMMA, Tadashi UEDA, Kenji HAMASE, Accumulation of D-amino acid residues in various proteins during storage, 45th International Symposium on High Performance Liquid Phase Separations and Related Techniques, 2017.06.
14. Chiharu ISHII, Shoto ISHIGO, Tetsuya MIYAMOTO, Yurika MIYOSHI, Masashi MITA, Hiroshi HOMMA, Tadashi UEDA, Kenji HAMASE, Determination of D-amino acid residues in proteins stored under various pH conditions, 27th International Symposium on Pharmaceutical and Biomedical Analysis, 2016.11.


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