Hiroaki Takesue | Last modified date:2023.07.04 |
Undergraduate School
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Homepage
https://kyushu-u.pure.elsevier.com/en/persons/hiroaki-takesue
Reseacher Profiling Tool Kyushu University Pure
Phone
092-642-6100
Academic Degree
Ph.D. (Pharmaceutical Science)
Country of degree conferring institution (Overseas)
No
Field of Specialization
Genome science, Molecular biology, Epigenetics, Pharmacokinetics
ORCID(Open Researcher and Contributor ID)
0000-0001-8585-5494
Total Priod of education and research career in the foreign country
00years00months
Research
Research Interests
Membership in Academic Society
- Knockout of RTT109 accelerates expansion of CUP1 arrays in budding yeast
keyword : Saccharomyces cerevisiae, CUP1, Cas9, histone modification, copy number variation
2022.04~2022.04. - Understanding the expansion mechanism of tandem repeats by a novel method using Cas9 nickase
keyword : Saccaromyces cerevisiae, tandem repeat, CUP1, Cas9, copy number variation
2021.04~2022.04.
Reports
- The Japan Society for Epigenetics
- Japan Society of Bioscience, Biotechnology, and Agrichemistry
- The Molecular Biology Society of Japan
- Molecular mechanisms of gene amplification are diverse, and most remain elusive. Accordingly, the application of gene amplification to construct stable gene expression systems is insufficient. We have developed a novel gene amplification method “BiTREx” based on a defined molecular mechanism that is controllable and does not require selection pressure, using budding yeast as a model. BiTREx stems from the fact that a nick introduced by Cas9 nickase causes replisome disassembly to generate a single-end double-strand break to be repaired by break-induced replication. Suppose the replisome that has just replicated the target tandem repeat encounters a nick at its flanking site. In that case, BIR should frequently initiate thorough non-allelic single-strand DNA invasion to expand the tandem repeat. Indeed, BiTREx successfully expanded a CUP1 array consisting of 16 copies of 2 kb repeat units to ~500 repeat units (~1 Mb). Furthermore, BiTREx successfully expanded not only another natural tandem gene array (ENA1 array) but artificial ones consisting of ~10-kb repeat units inserted at the CUP1 locus. Thus, BiTREx is a versatile method to enable various novel applications in synthetic genomics. We will also show a seamless system for gene integration followed by BiTREx in this symposium, and present the possibility of applying gene amplification by BiTREx to various researches.
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