Faculty Profiles - IKEGAMI YASUHIRO

Information

写真a

IKEGAMI YASUHIRO

Organization

Faculty of Engineering Department of Chemical Engineering Assistant Professor
School of Engineering （Concurrent）
Graduate School of Engineering （Concurrent）

Contact information

Tel

0928022768

Homepage

http://www.chem-eng.kyushu-u.ac.jp/lab8/
Laboratory of Biomaterial & Medical Engineering (Ijima Lab.) HP

External link

Degree

Doctor of Engineering

Research History

2018年4月日本学術振興会特別研究員（DC1） 2021年4月東京大学生産技術研究所物質・環境系部門特任研究員 2022年4月 Beyond AI研究推進機構特任研究員

Research Interests・Research Keywords

Research theme： Development of medical devices using functional biomaterials

Keyword： Electrospun nanofiber, Extracellular matrix-modeled material

Research period： 2017.4

Awards

日本バイオマテリアル学会 2020 年度九州ブロック学術講演会優秀賞

2021.1 末梢神経再生に向けたヘパリン導入ファイバーの開発と増殖因子シグナリング
化学工学会第 51 回秋季大会優秀ポスター賞

2020.9 ヘパリン導入ファイバーの開発と固定化増殖因子による末梢神経再生

Papers

Correction to: Heparin/Growth Factors-Immobilized Aligned Electrospun Nanofibers Promote Nerve Regeneration in Polycaprolactone/Gelatin-Based Nerve Guidance Conduits Reviewed

Yasuhiro Ikegami, Muhammad Shafiq, Shinichi Aishima, Hiroyuki Ijima

Advanced Fiber Materials 5 ( 2 ) 696 - 696 2023.2

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DOI： 10.1007/s42765-023-00261-z
Exploiting synergistic effect of externally loaded bFGF and endogenous growth factors for accelerated wound healing using heparin functionalized PCL/gelatin co-spun nanofibrous patches

Akshat Joshi, Zhe Xu, Yasuhiro Ikegami, Kozue Yoshida, Yusuke Sakai, Akshay Joshi, Tejinder Kaur, Yosuke Nakao, Yo-ichi Yamashita, Hideo Baba, Shinichi Aishima, Neetu Singh, Hiroyuki Ijima

Chemical Engineering Journal 404 126518 - 126518 2021.1

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Growth factors (GFs) are signaling molecules that are principle mediators in tissue regeneration. Biomaterial scaffolds employed as wound dressings are often hampered by their limitations to deliver GFs exogenously due to their instability and low half-life. The key to overcome this challenge lies in the better organization and use of endogenous pro-regenerative GFs released at regenerative site, with an aim to minimize the sole dependency on exogenous factors. Considering such challenges, this research utilizes the exogenous and endogenous GFs sequestering capability of heparin functionalized PCL/gelatin co-spun nanofabrics to mediate synergistically driven tissue regeneration by utilizing combined therapeutic effect of exogenous and endogenous GFs, and thereby minimizing the sole dependency on exogenous GFs for tissue regeneration. Basic fibroblast growth factor (bFGF) was chosen as GF for exogenous loading whereas vascular endothelial growth factor (VEGF) was chosen as a representative example to demonstrate the endogenous pro-regenerative GF sequestration capability of fabricated nanofabrics. From our results, the fabricated nanofabrics showed loading efficiency of 80% for exogenous bFGF and can sequester 15-fold more amount of endogenous VEGF compared to non-heparin functionalized nanofibrous dressings. When applied as wound dressings, heparin functionalized nanofibers showed better therapeutic capability compared to control groups that were treated using patches without heparin functionalization, indicating endogenously driven tissue regeneration. This was indicated by significant higher number of newly formed skin appendages, lesser scarring and lower inflammatory levels in newly formed granulation. Additionally, further improvements in therapeutic effect was observed when exogenous bFGF was employed indicating effectiveness of synergistically mediated tissue regeneration.

DOI： 10.1016/j.cej.2020.126518
Heparin-conjugated collagen as a potent growth factor-localizing and stabilizing scaffold for regenerative medicine Reviewed

Yasuhiro Ikegami, Hideyuki Mizumachi, Kozue Yoshida, Hiroyuki Ijima

Regenerative Therapy 15 236 - 242 2020.12

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DOI： 10.1016/j.reth.2020.10.002
Development of heparin-conjugated nanofibers and a novel biological signal by immobilized growth factors for peripheral nerve regeneration Reviewed

Yasuhiro Ikegami, Hiroyuki Ijima

Journal of Bioscience and Bioengineering 129 ( 3 ) 354 - 362 2020.3

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DOI： 10.1016/j.jbiosc.2019.09.004
Complex activity and short-term plasticity of human cerebral organoids reciprocally connected with axons Reviewed International journal

Tatsuya Osaki, Tomoya Duenki, Siu Yu A. Chow, Yasuhiro Ikegami, Romain Beaubois, Timothée Levi, Nao Nakagawa-Tamagawa, Yoji Hirano, Yoshiho Ikeuchi

Nature Communications 15 2945 2024.4

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Complex activity and short-term plasticity of human cerebral organoids reciprocally connected with axons
An inter-regional cortical tract is one of the most fundamental architectural motifs that integrates neural circuits to orchestrate and generate complex functions of the human brain. To understand the mechanistic significance of inter-regional projections on development of neural circuits, we investigated an in vitro neural tissue model for inter-regional connections, in which two cerebral organoids are connected with a bundle of reciprocally extended axons. The connected organoids produced more complex and intense oscillatory activity than conventional or directly fused cerebral organoids, suggesting the inter-organoid axonal connections enhance and support the complex network activity. In addition, optogenetic stimulation of the inter-organoid axon bundles could entrain the activity of the organoids and induce robust short-term plasticity of the macroscopic circuit. These results demonstrated that the projection axons could serve as a structural hub that boosts functionality of the organoid-circuits. This model could contribute to further investigation on development and functions of macroscopic neuronal circuits in vitro.

DOI： 10.1038/s41467-024-46787-7
A simple and inexpensive laser dissection of fasciculated axons from motor nerve organoids Reviewed

Yasuhiro Ikegami, Tomoya Duenki, Ikuma Arakaki, Ryo Sakai, Tatsuya Osaki, Satoshi Ashihara, Tsuyoshi Furushima, Yoshiho Ikeuchi

Frontiers in Bioengineering and Biotechnology 12 2024.1

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Motor nerve organoids could be generated by culturing a spheroid of motor neurons differentiated from human induced pluripotent stem (iPS) cells within a polydimethylsiloxane (PDMS) chip which guides direction and fasciculation of axons extended from the spheroid. To isolate axon bundles from motor nerve organoids, we developed a rapid laser dissection method based on localized photothermal combustion. By illuminating a blue laser on a black mark on the culture device using a dry-erase marker, we induced highly localized heating near the axon bundles. Moving the laser enabled spatial control over the local heating and severing of axon bundles. This laser dissection requires a black mark, as other colors did not produce the same localized heating effect. A CO₂ laser destroyed the tissue and the device and could not be used. With this simple, economical laser dissection technique, we could rapidly collect abundant pure axon samples from motor nerve organoids for biochemical analysis. Extracted axonal proteins and RNA were indistinguishable from manual dissection. This method facilitates efficient axon isolation for further analyses.

DOI： 10.3389/fbioe.2024.1259138
Efficacy of a newly developed bioabsorbable pancreatic clip for distal pancreatectomy in swine

Yo-ichi Yamashita, Takanobu Yamao, Yosuke Nakao, Tatsunori Miyata, Yasuhiro Ikegami, Soichiro Yamane, Taiga Ito, Taku Furukawa, Jaeyong Cho, Fanqi Wu, Yasumitsu Fujie, Mitsuru Arima, Shinichi Aishima, Hiroyuki Ijima, Hideo Baba

Surgery Today 52 ( 7 ) 1109 - 1114 2022.1

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PURPOSE: At present, ≥ 20% of patients experience clinically relevant postoperative pancreatic fistula (POPF) after distal pancreatectomy (DP). METHODS: We developed a new bioabsorbable pancreatic clip (BioPaC) made of polycaprolactone that does not crush the pancreatic parenchyma during occlusion of the pancreatic stump. We confirmed the efficacy of this BioPac in a porcine DP model and compared it to a linear stapling device (Reinforce®). RESULTS: Pigs were killed at 1 month after DP. In the BioPaC group, all swine (n = 3) survived well without POPF. In the Reinforce® group (n = 2), one pig died early at postoperative day 7 with Grade C POPF (amylase 43 700 U/l), and the other survived until 1 month at scarification with biochemical leakage of POPF (amylase 3 725 U/l). Pathologically, the main pancreatic duct and pancreatic parenchyma were well closed by BioPaC. CONCLUSION: The newly developed BioPaC is effective in a porcine DP model.

DOI： 10.1007/s00595-021-02435-x
Mito-FUNCAT-FACS reveals cellular heterogeneity in mitochondrial translation

Yusuke Kimura, Hironori Saito, Tatsuya Osaki, Yasuhiro Ikegami, Taisei Wakigawa, Yoshiho Ikeuchi, Shintaro Iwasaki

2022.1

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<jats:title>Abstract</jats:title><jats:p>Mitochondria possess their own genome that encodes components of oxidative phosphorylation (OXPHOS) complexes, and mitochondrial ribosomes within the organelle translate the mRNAs expressed from mitochondrial genome. Given the differential OXPHOS activity observed in diverse cell types, cell growth conditions, and other circumstances, cellular heterogeneity in mitochondrial translation can be expected. Although individual protein products translated in mitochondria have been monitored, the lack of techniques that address the variation in overall mitochondrial protein synthesis in cell populations poses analytic challenges. Here, we adapted mitochondrial-specific fluorescent noncanonical amino acid tagging (FUNCAT) for use with fluorescence-activated cell sorting (FACS) and developed mito-FUNCAT-FACS. The click chemistry-compatible methionine analog L-homopropargylglycine (HPG) enabled the metabolic labeling of newly synthesized proteins. In the presence of cytosolic translation inhibitors, HPG was selectively incorporated into mitochondrial nascent proteins and conjugated to fluorophores via the click reaction (mito-FUNCAT). The application of <jats:italic>in situ</jats:italic> mito-FUNCAT to flow cytometry allowed us to disentangle changes in net mitochondrial translation activity from those of the organelle mass and detect variations in mitochondrial translation in cancer cells. Our approach provides a useful methodology for examining mitochondrial protein synthesis in individual cells.</jats:p>

DOI： 10.1101/2022.01.03.474764
Creation of a novel lipid-trehalose derivative showing positive interaction with the cell membrane and verification of its cytoprotective effect during cryopreservation

Kozue Yoshida, Fumiyasu Ono, Takehiro Chouno, Shota Nakada, Yasuhiro Ikegami, Nana Shirakigawa, Yusuke Sakai, Hiroyuki Ijima

Journal of Bioscience and Bioengineering 132 ( 1 ) 71 - 80 2021.7

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Cryopreservation is important for enabling long-term cell preservation. However, physical damage due to ice crystal formation and membrane permeation by dimethyl sulfoxide (DMSO) severely affects cryopreserved cell viability. To ensure cell survival and functional maintenance after cryopreservation, it is important to protect the cell membrane, the most vulnerable cell component, from freeze-thaw damage. This study aimed to create a glycolipid derivative having a positive interaction with the cell membrane and cytoprotective effects. As a result, we synthesized a novel trehalose derivative, oleyl-trehalose (Oleyl-Treh), composed of trehalose and oleyl groups. Its use led to increased viable cell counts when used with DMSO in a non-cytotoxic concentration range (1.6 nM-16 μM). Oleyl-Treh significantly improved viability and liver-specific functions of hepatocytes after cryopreservation, including albumin secretion, ethoxyresorufin-O-deethylase activity (an indicator of cytochrome P450 family 1 subfamily A member 1 activity), and ammonia metabolism. Oleyl-Treh could localize trehalose to the cell membrane; furthermore, the oleyl group affected cell membrane fluidity and exerted cryoprotective effects. This novel cryoprotective agent, which shows a positive interaction with the cell membrane, provides a unique approach toward cell protection during cryopreservation.

DOI： 10.1016/j.jbiosc.2021.03.010
Development of a gel-in-oil emulsion as a transdermal drug delivery system for successful delivery of growth factors

Jannatul Fardous, Emiko Yamamoto, Yuji Omoso, Seiya Nagao, Yuuta Inoue, Kozue Yoshida, Yasuhiro Ikegami, Yi Zhang, Nana Shirakigawa, Fumiyasu Ono, Hiroyuki Ijima

Journal of Bioscience and Bioengineering 132 ( 1 ) 95 - 101 2021.7

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Growth factors (GFs) are indispensable in regenerative medicine because of their high effectiveness. However, as GFs degenerate easily, the development of a suitable carrier with improved stability for GFs is necessary. In this study, we developed a gel-in-oil (G/O) emulsion technology for the transdermal delivery of growth factors. Nanogel particles prepared with heparin-immobilized gelatin that can bind growth factors were dispersed in isopropyl myristate. The particle size of the G/O emulsion could be controlled by changing the surfactant concentration, volume ratio of the water phase to the oil phase, and gelatin concentration. In vitro skin penetration studies showed better penetration through the stratum corneum of fluorescent proteins containing G/O emulsions than of the aqueous solution of GF. Similarly, an in vivo study showed an angiogenesis-inducing effect after transdermal application of GF-immobilized G/O emulsion. Angiogenesis in mice was confirmed owing to both an increased blood vessel network and higher hemoglobin content in the blood. Therefore, the G/O emulsion could be a promising carrier for GFs with better stability and can effectively deliver GFs at the target site.

DOI： 10.1016/j.jbiosc.2021.03.015
Normothermic machine perfusion system satisfying oxygen demand of liver could maintain liver function more than subnormothermic machine perfusion

Kozue Yoshida, Shunsuke Nakamura, Hiroki Sakamoto, Mika Kondo, Takehiro Chouno, Yasuhiro Ikegami, Nana Shirakigawa, Hiroshi Mizumoto, Yo-ichi Yamashita, Hideo Baba, Hiroyuki Ijima

Journal of Bioscience and Bioengineering 131 ( 1 ) 107 - 113 2021.1

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Liver transplantation plays an important role in the medical field. To improve the quality of a donor liver, there is a need to establish a preservation system to prevent damage and maintain liver function. In response to this demand, machine perfusion (MP) has been proposed as a new liver preservation method instead of the conventional static cold storage. There is controversy about the optimal MP temperature of the donor liver. Since the oxygen consumption of the liver differs depending on the temperature, construction of a system that satisfies the oxygen demand of the liver is crucial for optimizing the preservation temperature. In this study, an MP system, which satisfies the oxygen demand of liver at each temperature, was constructed using an index of oxygen supply; the overall volumetric oxygen transfer coefficient, the amount of oxygen retention of perfusate and oxygen saturation. Both subnormothermic MP (SNMP, 20-25 degrees C) and normothermic MP (NMP, 37 degrees C) could maintain liver viability at a high level (94%). However, lactate metabolism of the liver during NMP was more active than that during SNMP. Furthermore, the ammonia metabolism of liver after NMP was superior to that after SNMP. Hence, NMP, which maintains the metabolic activity of the liver, is more suitable for preservation of the donor liver than SNMP, which suppresses the metabolic activity. In summary, normothermia is the optimal temperature for liver preservation, and we succeeded in constructing an NMP system that could suppress liver damage and maintain function. (C) 2020, The Society for Biotechnology, Japan. All rights reserved.

DOI： 10.1016/j.jbiosc.2020.08.011
Cryoprotective enhancing effect of very low concentration of trehalose on the functions of primary rat hepatocytes International journal

Kozue Yoshida, Fumiyasu Ono, Takehiro Chouno, Bual Ronald Perocho, Yasuhiro Ikegami, Nana Shirakigawa, Hiroyuki Ijima

Regenerative Therapy 15 173 - 179 2020.12

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Introduction: Cells have various applications in biomedical research. Cryopreservation is a cell-preservation technique that provides cells for such applications. After cryopreservation, sensitive cells, such as primary hepatocytes, suffer from low viability due to the physical damage caused by ice crystals, highlighting the need for better methods of cryopreservation to improve cell viability. Given the importance of effectively suppressing ice crystal formation to protect cellular structure, trehalose has attracted attention as cryoprotectant based on its ability to inhibit ice crystal formation; however, trehalose induces osmotic stress. Therefore, to establish a cell-cryopreservation technique, it is necessary to provide an optimal balance between the protective and damaging effects of trehalose. Methods: In this study, we evaluated the effects of osmotic stress and ice crystal formation on the viability and function of primary rat hepatocytes at wide range of trehalose concentration. Results: There was no osmotic stress at very low concentrations (2.6 μM) of trehalose, and 2.6 μM trehalose drives the formation of finer ice crystals, which are less damaging to the cell membrane. Furthermore, we found that the number of viable hepatocytes after cryopreservation were 70% higher under the 2.6 μM trehalose-supplemented conditions than under the dimethyl sulfoxide-supplemented conditions. Moreover, non-cryopreserved cells and cells cryopreserved with trehalose showed comparable intracellular dehydrogenase activity. Conclusions: We showed that trehalose at very low concentrations (2.6 μM) improved dramatically viability and liver function of hepatocyte after cryopreservation.

DOI： 10.1016/j.reth.2020.08.003
Co-culture of mesenchymal stem cells and human umbilical vein endothelial cells on heparinized polycaprolactone/gelatin co-spun nanofibers for improved endothelium remodeling

Akshat Joshi, Zhe Xu, Yasuhiro Ikegami, Soichiro Yamane, Masanori Tsurashima, Hiroyuki Ijima

International Journal of Biological Macromolecules 151 186 - 192 2020.5

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DOI： 10.1016/j.ijbiomac.2020.02.163
Analysis of Sulfated Glycosaminoglycans in ECM Scaffolds for Tissue Engineering Applications: Modified Alcian Blue Method Development and Validation

Tuyajargal Iimaa, Yasuhiro Ikegami, Ronald Bual, Nana Shirakigawa, Hiroyuki Ijima

Journal of Functional Biomaterials 10 ( 2 ) 19 - 19 2019.4

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<jats:p>Accurate determination of the amount of glycosaminoglycans (GAGs) in a complex mixture of extracellular matrix (ECM) is important for tissue morphogenesis and homeostasis. The aim of the present study was to investigate an accurate, simple and sensitive alcian blue (AB) method for quantifying heparin in biological samples. A method for analyzing heparin was developed and parameters such as volume, precipitation time, solvent component, and solubility time were evaluated. The AB dye and heparin samples were allowed to react at 4 ℃ for 24 h. The heparin-AB complex was dissolved in 25 N NaOH and 2-Aminoethanol (1:24 v/v). The optical density of the solution was analyzed by UV-Vis spectrometry at 620 nm. The modified AB method was validated in accordance with U.S. Food and Drug Administration guidelines. The limit of detection was found to be 2.95 µg/mL. Intraday and interday precision ranged between 2.14–4.83% and 3.16–7.02% (n = 9), respectively. Overall recovery for three concentration levels varied between 97 ± 3.5%, confirming good accuracy. In addition, this study has discovered the interdisciplinary nature of protein detection using the AB method. The basis for this investigation was that the fibrous protein inhibits heparin-AB complex whereas globular protein does not. Further, we measured the content of sulfated GAGs (sGAGs; expressed as heparin equivalent) in the ECM of decellularized porcine liver. In conclusion, the AB method may be used for the quantitative analysis of heparin in ECM scaffolds for tissue engineering applications.</jats:p>

DOI： 10.3390/jfb10020019
Fabrication of liver-derived extracellular matrix nanofibers and functional evaluation in in vitro culture using primary hepatocytes

Ronald Bual, Haruna Kimura, Yasuhiro Ikegami, Nana Shirakigawa, Hiroyuki Ijima

Materialia 4 518 - 528 2018.12

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Fabrication of functional scaffolds is one of the main goals of tissue engineering, particularly scaffolds mimicking the native extracellular matrix (ECM) and stimulating a microenvironment that supports cell growth and function. In this study, a scaffold was developed using liver-derived ECM (L-ECM), gelatin, and polycaprolactone (PCL) by conventional electrospinning. This study aimed to improve the gelatin-PCL nanofiber blend by incorporating L-ECM. The morphology of the nanofibrous scaffold was investigated by scanning electron microscopy. Characterizations such as Fourier transform infrared spectroscopy (FTIR), tensile strength test, and water contact angle analysis revealed the favorable characteristics of the scaffold. Particularly, the FTIR spectra confirmed the presence of L-ECM, gelatin, and PCL, even after crosslinking treatment, while tensile strength analysis revealed suitable mechanical properties and water contact angle measurement showed the improved hydrophilic characteristics of the scaffold. The biocompatibility of the composite nanofibers was evaluated by in vitro culture of primary hepatocytes. Phase-contrast microscope images verified that the L-ECM nanofibers improved hepatocyte adhesion and facilitated the formation of a tissue-like structure. Moreover, high liver-specific functions were obtained in L-ECM nanofibers compared to gelatin/PCL nanofibers. Thus, L-ECM nanofibers can be used in tissue engineering, particularly in liver regeneration.

DOI： 10.1016/j.mtla.2018.11.014
Development of an in situ evaluation system for neural cells using extracellular matrix-modeled gel culture

Takayuki Nagai, Yasuhiro Ikegami, Hideyuki Mizumachi, Nana Shirakigawa, Hiroyuki Ijima

Journal of Bioscience and Bioengineering 124 ( 4 ) 430 - 438 2017.10

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Two-dimensional monolayer culture is the most popular cell culture method. However, the cells may not respond as they do in vivo because the culture conditions are different from in vivo conditions. However, hydrogel-embedding culture, which cultures cells in a biocompatible culture substrate, can produce in vivo-like cell responses, but in situ evaluation of cells in a gel is difficult. In this study, we realized an in vivo-like environment in vitro to produce cell responses similar to those in vivo and established an in situ evaluation system for hydrogel-embedded cell responses. The extracellular matrix (ECM)-modeled gel consisted of collagen and heparin (Hep-col) to mimic an in vivo-like environment. The Hep-col gel could immobilize growth factors, which is important for ECM functions. Neural stem/progenitor cells cultured in the Hep-col gel grew and differentiated more actively than in collagen, indicating an in vivo-like environment in the Hep-col gel. Second, a thin-layered gel culture system was developed to realize in situ evaluation of the gel-embedded cells. Cells in a 200-μm-thick gel could be evaluated clearly by a phase-contrast microscope and immunofluorescence staining through reduced optical and diffusional effects. Finally, we found that the neural cells cultured in this system had synaptic connections and neuronal action potentials by immunofluorescence staining and Ca2+ imaging. In conclusion, this culture method may be a valuable evaluation system for neurotoxicity testing.

DOI： 10.1016/j.jbiosc.2017.04.018

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Books

Immobilization Strategies: Biomedical, Bioengineering and Environmental Applications -Strategies and advancement in growth factor immobilizable ECM for tissue engineering-

Yasuhiro Ikegami, Hiroyuki Ijima（Role：Joint author）

Springer 2023.6

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DOI： https://doi.org/10.1007/978-981-15-7998-1_3
Decellularization of Tissue and Whole Organs in Tissue Engineering -Decellularization of Nervous Tissues and Efforts for their Clinical Application-

Yasuhiro Ikegami, Hiroyuki Ijima（Role：Joint author）

Springer 2021.9

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DOI： https://doi.org/10.1007/978-3-030-82735-9_19

Other Link： https://link.springer.com/content/pdf/10.1007/978-3-030-82735-9_19
エレクトロスプレー/スピニング法とその応用 : 材料合成・成形・加工技術

山下義裕福井大学平岡賢三山梨大学内藤親視㈱メック後藤元信名古屋大学ワーユディオノ名古屋大学シティマームダー名古屋大学, Institut Teknologi Sepuluh Nopember 髙﨑緑京都工芸繊維大学田中克史京都工芸繊維大学小林治樹京都工芸繊維大学竹入啓二ナガセテクノエンジニアリング㈱浦木康光北海道大学宮瑾山形大学藤田聡福井大学吉田裕安材奈良先端科学技術大学院大学黒川成貴慶應義塾大学前田知貴茨城大学, 慶應義塾大学堀田篤慶應義塾大学坂元博昭福井大学西籔和明近畿大学岸本吉則㈱ファイバーマトリックス研究所向井康人名古屋大学朱春紅信州大学金翼水信州大学赤坂修一東京工業大学川上浩良東京都立大学内田誠山梨大学堀江雄二鹿児島大学清家善之愛知工業大学高橋泰樹工学院大学工藤幸寛工学院大学石井佑弥京都工芸繊維大学川上亘作物質・材料研究機構中村耕一郎日本毛織㈱, 京都大学田畑泰彦京都大学池上康寛九州大学井嶋博之九州大学小幡亜希子名古屋工業大学春日敏宏名古屋工業大学東城武彦花王㈱成島毅花王㈱内山雅普花王㈱玉田靖信州大学成瀬大輔第一編物㈱吉田巧富山県産業技術研究開発センター金丸亮二 (公財)富山県新世紀産業機構（Role：Joint author）

シーエムシー出版 2021.3

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Responsible for pages：総ページ数：9,341p Language：Japanese

Presentations

末梢神経再生に向けたヘパリン導入ファイバーの開発と増殖因子シグナリング

池上康寛, 小柳和也, 伊藤泰我, 堺裕輔, 井嶋博之

日本バイオマテリアル学会２０２０年度九州ブロック学術講演会 2021.1

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ヘパリン導入ファイバーの開発と固定化増殖因子による末梢神経再生

池上康寛, 小柳和也, 吉田梢, 堺裕輔, 井嶋博之

化学工学会第51回秋季大会 2020.9

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Alignment and immobilized neurotrophic factors of the heparin-functionalized nanofibrous scaffold provide synergistic effects on the peripheral nerve regeneration

Yasuhiro Ikegami, Hiroyuki Ijima

6th Nano Today Conference 2019.6

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長距離神経欠損再生に向けた機能性分子固定化能を有する繊維性足場基材の開発

池上康寛, 堺裕輔, 井嶋博之

第55回九大生体材料・力学研究会 2019.12

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Development of heparin-conjugated ECM fibers for peripheral nerve regeneration across a long gap with controlled neurites outgrowth

Yasuhiro Ikegami, Nana Shirakigawa, Hiroyuki Ijima

5th TERMIS World Congress 2018 2018.9

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ヘパリン修飾ファイバーの開発と末梢神経再生のための複合シグナリング

池上康寛, 白木川奈菜, 井嶋博之

化学工学会第50回秋季大会 2018.9

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ヘパリン導入ECM基材開発による神経細胞培養

池上康寛, 長尾征哉, 水町秀之, 白木川奈菜, 井嶋博之

第54回化学関連支部合同九州大会 2017.7

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機能性バイオマテリアルとしてのヘパリン導入ECM基材の開発

池上康寛, 水町秀之, 叶婧佳, 白木川奈菜, 井嶋博之

第23回日本生物工学会九州支部飯塚大会 2016.12

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神経系細胞培養のための脳特異的ECM模倣基材としてのヘパリン導入コラーゲン

池上康寛, 永井貴之, 原田祐希, 白木川奈菜, 井嶋博之

第18回化学工学会学生発表会(福岡大会) 2016.3

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ヘパリンとコラーゲンから成るECM模倣培養基材の開発

池上康寛, 永井貴之, 原田祐希, 白木川奈菜, 井嶋博之

第22回日本生物工学会九州支部宮崎大会 2015.12

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尾側膵切除に用いる生体吸収膵臓クリップの開発ブタを用いたFeasibility試験

山下洋市, 山尾宣暢, 中尾陽佑, 池上康寛, 伊藤泰我, 呉帆き, 池田哲夫, 井嶋博之, 馬場秀夫

日本内視鏡外科学会雑誌 2021.12

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消化器外科における医工連携の現状と展望医工連携研究によるもの創り生体吸収性膵臓クリップの開発現状と展望

山下洋市, 山尾宣暢, 中尾陽佑, 池上康寛, 山根颯一郎, 伊藤泰我, 古川拓, 池田哲夫, 井嶋博之, 馬場秀夫

日本消化器外科学会総会 2021.7

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膵液瘻に対するマネージメント膵液瘻ゼロを目指した膵切離法に関する基礎的検討生体吸収素材でできた膵臓クリップの開発現状

山下洋市, 山尾宣暢, 井嶋博之, 山根颯一郎, 池上康寛, 中尾陽佑, 池田哲夫, 馬場秀夫

日本臨床外科学会雑誌 2020.10

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膵液瘻に対するマネージメント膵液瘻ゼロを目指した膵切離法に関する基礎的検討生体吸収素材でできた膵臓クリップの開発現状

山下洋市, 山尾宣暢, 井嶋博之, 山根颯一郎, 池上康寛, 中尾陽佑, 池田哲夫, 馬場秀夫

日本臨床外科学会雑誌 2020.10

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B205 観察/評価の容易さと生体内類似環境を両立した神経系細胞培養技術(B2-1 細胞バイオメカニクス4(細胞外マトリックス))

永井貴之, 原田祐希, 池上康寛, 水町秀之, 白木川奈菜, 井嶋博之

バイオフロンティア講演会講演論文集 2015.10

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B205 The novel culture technology of neural stem cells consist of the easy observable/evaluable device with in vivo-like environment
増殖因子内包ナノゲルエマルションの経皮送達による血管新生誘導効果

張怡, 重栖佑次, 井上雄太, Fardous Jannatul, 池上康寛, 堺裕輔, 小野文靖, 井嶋博之

2021年日本生体医工学会九州支部学術講演会 2021.3

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臨床応用を目指した肝胆膵領域における医工連携研究の現状と展望

山下洋市, 中尾陽佑, 山尾宣暢, 宮田辰徳, 福田有嘉子, 中田捷太, 池上康寛, 白木川奈菜, 井嶋博之, 馬場秀夫

JDDW 2021 KOBE_(第29回日本消化器関連学会週間, Japan Digestive disease week 2021) 2021.11

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⼈工神経⾜場基材としての神経系ECMファイバーの開発

小柳和也, 池上康寛, 堺裕輔, 井嶋博之

化学工学会九州支部オンライン学生発表会 2020.12

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コアシャルナノファイバーからなる組織工学的人工血管の開発

徐喆, Joshi Akshat, 池上康寛, 貫島匡生, 堺裕輔, 井嶋博之

第26回日本生物工学会九州支部長崎大会 2019.12

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薬物送達製剤としての生体適合性ナノゲル粒子の開発

重栖佑次, 山本惠美子, 池上康寛, 小野文靖, 井嶋博之

第56回化学関連支部合同九州大会 2019.7

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Development of liver-specific ECM electrospun nanofiber as a potential substrate for primary hepatocytes

Ronald Perocho Bual, Haruna Kimura, Yasuhiro Ikegami, Nana Shirakigawa, Hiroyuki Ijima

5th TERMIS World Congress 2018 2018.9

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生体組織置換可能な静電紡糸チューブ構造体の開発による人工胆管構築の試み

山根颯一郎, 池上康寛, 白木川奈菜, 井嶋博之

化学工学会第50回秋季大会 2018.9

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移植可能な組織工学的人工胆管の開発

山根颯一郎, 森保紘樹, 池上康寛, 白木川奈菜, 井嶋博之

2018年日本生体医工学会九州支部学術講演会 2018.3

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縫合可能な組織工学的人工胆管の開発と胆管上皮細胞の取得に関する検討

山根颯一郎, 池上康寛, 白木川奈菜, 山尾宣暢, 宮田辰徳, 山下洋市, 馬場秀夫, 井嶋博之

第55回化学関連支部合同九州大会 2018.6

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ヘパリン固定化基材からなる吻合可能な組織工学的チューブ構造体の開発

山根颯一郎, 森保紘樹, 池上康寛, 白木川奈菜, 井嶋博之

第7回日本バイオマテリアル学会九州ブロック講演会 2017.12

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機能性足場基材と共培養組織体から成る再細胞化肝臓の開発

植田翔平, 池上康寛, 木村遥奈, 白木川奈菜, 井嶋博之

第54回化学関連支部合同九州大会 2017.7

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肝再生医療のための足場基材および共培養組織体作製法の開発

植田翔平, 池上康寛, 木村遥奈, 永井貴之, 趙宰庸, 白木川奈菜, 井嶋博之

2017年日本生体医工学会九州支部学術講演会 2017.3

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血管新生を誘導する移植用基材の開発と毛包原基作製に関する検討

佐々木慎太郎, 池上康寛, 白木川奈菜, 井嶋博之

第54回化学関連支部合同九州大会 2017.7

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ECM模倣基材を用いた薄層ゲル培養による新規神経系細胞培養系の開発

永井貴之, 原田祐希, 池上康寛, 白木川奈菜, 井嶋博之

2016年日本生体医工学会九州支部学術講演会 2016.3

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ミコセル®を用いた機能性細胞の三次元培養

井嶋博之, 池上康寛, 木村遥奈, 白木川奈菜, 水本博, 牧野朋未, 藤林輝久, 仲田拓馬, 堀川洋, 今瀬将人

第15回日本再生医療学会総会 2016.3

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観察/評価の容易さと生体内類似環境を両立した新規３次元培養技術

白木川奈菜, 原田祐希, 永井貴之, 池上康寛, 井嶋博之

第15回日本再生医療学会総会 2016.3

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ECM模倣基材を用いた薄層ゲル培養系構築による神経毒性試験

永井貴之, 原田祐希, 池上康寛, 白木川奈菜, 井嶋博之

第22回日本生物工学会九州支部宮崎大会 2015.12

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観察/評価の容易さと生体内類似環境を両立した神経系細胞培養技術

永井貴之, 原田祐希, 池上康寛, 水町秀之, 白木川奈菜, 井嶋博之

日本機械学会第26回バイオフロンティア講演会 2015.10

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Fabrication of artificial blood vessel using gelatin/PCL core-shell nanofiber

Xu Zhe, Joshi Aksha, Yasuhiro Ikegami, Nana Shirakigawa, Hiroyuki Ijima

第55回化学関連支部合同九州大会 2018.6

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Fabrication of artificial blood vessel using gelatin/PCL core-shell nanofiber
Quantitative determination of sGAG in natural ECM Scaffold for Liver Tissue Engineering

Iimaa Tuyajargal, Ikegami Yasuhiro, Bual Ronald, Shirakigawa Nana, Ijima Hiroyuki

化学工学会第50回秋季大会 2018.9

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Quantitative determination of sGAG in natural ECM Scaffold for Liver Tissue Engineering
3D culture of primary hepatocytes on L-ECM/gelatin/PCL electrospun nanofibers for tissue engineering applications

Ronald Bual, Haruna Kimura, Yasuhiro Ikegami, Nana Shirakigawa, Hiroyuki Ijima

第7回日本バイオマテリアル学会九州ブロック講演会 2017.12

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3D culture of primary hepatocytes on L-ECM/gelatin/PCL electrospun nanofibers for tissue engineering applications
Development of L-ECM/gelatin/PCL electrospun nanofibers as a potential substrate for primary hepatocytes in vitro

Ronald Bual, Haruna Kimura, Yasuhiro Ikegami, Nana Shirakigawa, Hiroyuki Ijima

第53回九大生体材料・力学研究会 2017.10

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Development of L-ECM/gelatin/PCL electrospun nanofibers as a potential substrate for primary hepatocytes in vitro
ECM モデルファイバーを用いた組織工学用足場の開発

池上康寛, 堺裕輔, 井嶋博之

第５６回九大生体材料・力学研究会 2024.3

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安定した薬物送達を実現するナノゲルキャリア－点眼による後眼部への送達・経皮送達・創傷治癒－

土井亨太, 廣田祐也, 張怡, 池上康寛, 宮庄拓, 樋口明弘, 井嶋博之

第11回TR推進合同フォーラム・ライフサイエンス技術交流会 2024.1

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湿式静電紡糸法を用いた細胞足場としてのPCL/Gelatin三次元ファイバー基材の開発

李正浩, 池上康寛, 西田元気, 堺裕輔, 井嶋博之

化学工学会第89年会 2024.3

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皮膜形成エマルションの開発・最適化とその経皮投与効果の評価

胡笳, 張怡, 廣田裕也, 池上康寛, 堺裕輔, 相島慎一, 井嶋博之

第23回日本再生医療学会総会 2024.3

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肝組織工学のためのECMモデル材料を基盤とした繊維性足場基材の開発

池上康寛, 安井恵吾, 西田元気, 堺裕輔, 井嶋博之

化学工学会第89年会 2024.3

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Artificial Nerve Guidance Conduits based onPolycaprolactone/Gelatin-based Core/Shell typeAligned Electrospun Fibers

Muhammad Shafiq, Kazuo Koyanagi, Yasuhiro Ikegami, Shinichi Aishima, Hiroyuki Ijima

2023.5

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Artificial Nerve Guidance Conduits based onPolycaprolactone/Gelatin-based Core/Shell typeAligned Electrospun Fibers
Hyaluronic Acid Double-Network Hydrogels Immobilized with bFGF for Wound Healing

Wu Lichun, Zhang Yi, Zhou Yu, Wang Jinli, 池上康寛, 堺裕輔, 井嶋博之

化学工学会第54回秋季大会 2023.9

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Hyaluronic Acid Double-Network Hydrogels Immobilized with bFGF for Wound Healing
Study on preparation and formulation of ceramide nanoemulsions

Zhou Yu, Zhang Yi, Wu Lichun, Hu Jia, 池上康寛, 堺裕輔, 井嶋博之

化学工学会第54回秋季大会 2023.9

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Study on preparation and formulation of ceramide nanoemulsions
ゲル分解制御及び高肝機能を実現するハイブリッドオルガノイドの検討

家村歩, 土光悟史, UeharaMario K, StefanLucija, 池上康寛, 堺裕輔, 井嶋博之

第59回化学関連支部合同九州大会 2023.7

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トレハロースを基盤とした初代肝細胞の凍結保存技術

井嶋博之, 水本博, 池上康寛

第61回日本人工臓器学会大会 2023.11

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ナノファイバー多層化創傷被覆材の開発と形態制御に基づいた最適化

茶堂直志, 隈元紘輝, 吴卓盈, 池上康寛, 堺裕輔, 井嶋博之

第 29 回日本生物工学会九州支部福岡大会（2023) 2023.12

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ヘパリン導入ナノファイバーを用いた肝細胞培養における間葉系幹細胞併用の効果

安井恵吾, 西田元気, 池上康寛, 堺祐輔, 井嶋博之

第 29 回日本生物工学会九州支部福岡大会（2023) 2023.12

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三次元細胞培養に向けたPCL/Gelatin 湿式静電紡糸繊維の形態制御

李正浩, 池上康寛, 堺裕輔, 井嶋博之

日本バイオマテリアル学会 2023年度九州ブロック研究発表会 2023.12

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増殖因子環境制御を実現する皮膚創傷被覆材の開発

隈元紘輝, 西田元気, 張怡, 池上康寛, 堺裕輔, 相島慎一, 井嶋博之

第59回化学関連支部合同九州大会 2023.7

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多重ナノゲルエマルション型薬物送達製剤の開発

廣田祐也, 土井亨太, 張怡, 池上康寛, 堺裕輔, 樋口明弘, 井嶋博之

化学工学会第54回秋季大会 2023.9

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多重ナノゲルエマルション型薬物送達製剤の開発

廣田祐也, 土井亨太, 張怡, 池上康寛, 堺裕輔, 井嶋博之

第59回化学関連支部合同九州大会 2023.7

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新型経皮投与としての皮膜形成エマルションの開発

胡笳, 張怡, 周愉, 池上康寛, 堺裕輔, 井嶋博之

日本バイオマテリアル学会 2023年度九州ブロック研究発表会 2023.12

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皮膜形成エマルションの開発とその経皮投与効果の評価

胡笳, 張怡, 土井亨太, Zhou Yu, 池上康寛, 堺裕輔, 井嶋博之

化学工学会第54回秋季大会 2023.9

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皮膜形成エマルションによる新規持続経皮投与システムの開発

胡笳, 張怡, 池上康寛, 堺裕輔, 井嶋博之

第59回化学関連支部合同九州大会 2023.7

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肝臓の灌流保存システムの構築

井嶋博之, 水本博, 池上康寛

第61回日本人工臓器学会大会 2023.11

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胆管がん治療を目的とした機能性ハイドロゲルの開発

王錦霖, 張怡, 武利春, 廣田祐也, 池上康寛, 堺裕輔, 井嶋博之

化学工学会第54回秋季大会 2023.9

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臓器鋳型への共培養組織体導入による再細胞化肝臓の構築

平井塁, UeharaMario K, StefanLucija, 土光悟史, 池上康寛, 堺裕輔, 井嶋博之

第59回化学関連支部合同九州大会 2023.7

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Development of a functional miniature liver based on the right lobe of mice

Lucija Stefan, Mario Kokichi Uehara, Fanqi Wu, Yukako Fukuda, Yo-Ichi Yamashita, Shinichi Aishima, Yasuhiro Ikegami, Yusuke Sakai, Hiroyuki Ijima

第23回日本再生医療学会総会 2024.3

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Development of a functional miniature liver based on the right lobe of mice

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Professional Memberships

化学工学会

Research Projects

神経工学を基盤とする新規がん治療戦略のための血管/自律神経/肝臓複合組織体の開発

2023 - 2024

令和5年度工学研究新分野開拓助成

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脳オルガノイド回路による統合失調症の発症機序の解明

2022.4 - 2025.3

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Authorship：Principal investigator

Educational Activities

演習および学生実験の指導
研究室学生の学位論文の指導

Class subject

化学工学実験第一

2024.10 - 2025.3 Second semester
化学工学実験第三

2024.10 - 2025.3 Second semester
化学工学実験第一

2023.10 - 2024.3 Second semester
化学工学実験第三

2023.10 - 2024.3 Second semester

FD Participation

2023.4 Role：Participation Title：令和５年度第１回全学ＦＤ（新任教員の研修）The 1st All-University FD (training for new faculty members) in FY2023

Organizer：University-wide