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JIALE MA Last modified date:2023.12.06

Assistant Professor / Division of Oral Health, Growth and Development
Department of Dental Science
Faculty of Dental Science

Graduate School
Undergraduate School

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 Reseacher Profiling Tool Kyushu University Pure
Academic Degree
Doctor of Philosophy (Dental Science)
Country of degree conferring institution (Overseas)
Field of Specialization
Preventive Dentistry
ORCID(Open Researcher and Contributor ID)
Total Priod of education and research career in the foreign country
Research Interests
  • Study of the relationship between oral microbiota and oral or systemic health
    keyword : Oral microbiome, 16S rRNA gene, Next generation sequence
Academic Activities
1. Shinya Kageyama, Jiale Ma, Michiko Furuta, Toru Takeshita, Mikari Asakawa, Yuka Okabe, Yoshihisa Yamashita, Establishment of tongue microbiota by 18 months of age and determinants of its microbial profile, mBio, 10.1128/mbio.01337-23, 2023.10, [URL], Acquisition of oral bacteria early in life is considered to have an important role in the establishment of oral microbiota. In this study, we examined tongue swab samples collected from 216 infants at the 18-month checkup, along with samples collected from them and from their mothers at the 4-month checkup. The bacterial composition of each sample was determined using PacBio single-molecule long-read sequencing of the full-length 16S rRNA gene and amplicon sequence variant (ASV) analysis. At 18 months of age, the tongue microbiota was dominated by Streptococcus salivarius, Neisseria perflava, and Granulicatella adiacens; and the overall bacterial composition was more similar to that of the mothers than at 4 months of age. The bacterial composition at 18 months of age was classified into three microbiota profiles: S. salivarius-dominant, Neisseria-dominant, and infant profile dominated by 4 months specific ASVs such as S. salivarius, Streptococcus lactarius, and Streptococcus peroris. Those with infant profiles exhibited significantly higher rates of current breastfeeding, dental caries or white spot lesions, and dental plaque accumulation compared to infants with the other profiles. Comparison between the S. salivarius- and Neisseria-dominant profiles showed that incomplete weaning, low intake of fruits, and frequent intake of sweetened beverages or sweet snacks were significantly associated with the S. salivarius-dominant profile. Our findings, based on a high-resolution profiling approach, demonstrate that the foundation of the adult tongue microbiota is established by 18 months of age, and the bacterial profile is possibly determined by dietary habits during this period. IMPORTANCE Understanding the development of oral microbiota early in life and the factors that influence it is important for preventing the establishment of dysbiotic oral microbiota later in life. This study demonstrates that the tongue microbiota undergoes early development from 4 to 18 months of age and converges into two types of microbiota showing indications of adult characteristics, with either S. salivarius or Neisseria-dominance. Interestingly, their divergence was strongly determined by their weaning status and the dietary frequencies of sweetened beverages, snacks, and fruits, suggesting that dietary habits during this period might influence the establishment of the oral microbiota. These findings may contribute to the development of novel preventive strategies against oral microbiota-related diseases..
2. Shinya Kageyama, Satoko Sakata, Jiale Ma, Mikari Asakawa, Toru Takeshita, Michiko Furuta, Toshiharu Ninomiya, Yoshihisa Yamashita, High-Resolution Detection of Translocation of Oral Bacteria to the Gut, Journal of Dental Research, 10.1177/00220345231160747, Open access, 2023.05, Ectopic enrichment of oral microbes in the gut is a notable alteration in gut microbial balance. These microbes are likely delivered from the oral cavity with saliva and food; however, evidence of oral-gut microbial transmission is insufficient and needs further investigation. In this observational study, we examined 144 pairs of saliva and stool samples collected from community-dwelling adults to verify the oral-gut microbial link and identify the relevant influencing factors on the increased abundance of oral microbes within the gut. The bacterial composition of each sample was determined using PacBio single-molecule long-read sequencing of the full-length 16S ribosomal RNA gene and amplicon sequence variant (ASV) analysis. Although the bacterial compositions of salivary and gut microbiota were distinctly different, at least 1 ASV was shared between salivary and gut microbiota in 72.9% of subjects. Shared ASVs accounted for 0.0% to 63.1% (median 0.14%) of the gut microbiota in each subject and frequently included abundant Streptococcus salivarius and Streptococcus parasanguinis. Their total relative abundance in the gut was significantly higher in older subjects or those with dental plaque accumulation. The gut microbiota with ≥5% of shared ASVs displayed a higher abundance of Streptococcus, Lactobacillus, and Klebsiella and a lower abundance of Faecalibacterium, Blautia, Megamonas, and Parabacteroides. Our study presents evidence for the translocation of oral bacteria to the gut in community-dwelling adults and suggests that aging and dental plaque accumulation contribute to an increased abundance of oral microbes in the gut, which might be relevant to the compositional shift in the gut commensals..
3. Jiale Ma, Michiko Furuta, Kazuhiro Uchida, Toru Takeshita, Shinya Kageyama, Mikari Asakawa, Kenji Takeuchi, Shino Suma, Satoko Sakata, Jun Hata, Woosung Sohn, Toshiharu Ninomiya, Yoshihisa Yamashita, Yogurt product intake and reduction of tooth loss risk in a Japanese community., Journal of clinical periodontology, 10.1111/jcpe.13593, 49, 4, 345-352, 2022.04, AIM: To evaluate the longitudinal association between yogurt product intake and oral health in a population-based study. MATERIALS AND METHODS: This study included 1967 Japanese residents aged 40-79 years who underwent dental examinations in 2012. Among them, 1469 participants were followed up in 2017 for the incidence of tooth loss, which was defined as two or more teeth lost over 5 years. The intake of yogurt products, defined as yogurt and lactic acid beverages, was estimated using a semi-quantitative food frequency questionnaire. The composition of the salivary microbiota was evaluated. RESULTS: The Poisson regression model showed that a higher intake of yogurt products was negatively associated with the incidence of tooth loss (p for trend = .020), adjusted for potential confounding factors. Mediation analysis confirmed that periodontal condition partly mediated the effect of yogurt product intake on tooth loss, while dental caries experience did not. Additionally, we confirmed the association of a high intake of yogurt products with a low percentage of the salivary microbiota pattern, which was associated with poor oral health. CONCLUSION: These findings suggest that the intake of yogurt products is associated with a lower risk of tooth loss resulting from periodontal disease, probably via modulation of the oral microbiome composition..
4. Shinya Kageyama, Michiko Furuta, Toru Takeshita, Jiale Ma, Mikari Asakawa, Yoshihisa Yamashita, High-Level Acquisition of Maternal Oral Bacteria in Formula-Fed Infant Oral Microbiota., mBio, 10.1128/mbio.03452-21, 13, 1, e0345221, 2022.02, The influx of maternal oral microbes is considered to play an important role in the acquisition and development of infant oral microbiota. In this study, we examined tongue swab samples from 448 mother-infant pairs at 4-month checkups. The bacterial composition of each sample was determined using PacBio single-molecule long-read sequencing of the full-length 16S rRNA gene and the amplicon sequence variant (ASV) approach. Although the infant oral microbiota was distinctly different from the mother oral microbiota, ASVs shared with their biological mother accounted for a median relative abundance of 9.7% (range of 0.0 to 99.3%), which was significantly higher than that of ASVs shared with unrelated mothers. This shared abundance was strongly associated with the feeding method of infants rather than their delivery mode or antibiotic exposure, and formula-fed infants had higher shared abundance than exclusively breastfed infants. Our study presents strain-level evidence for mother-to-infant transmission of oral bacteria and suggests that colonization of maternal oral bacteria is higher in formula-fed infants. IMPORTANCE Acquisition of oral bacteria during infancy can affect the subsequent formation of stable oral microbiota. This study focused on the mother-to-infant transmission of oral bacteria, a major acquisition route of infant oral microbiota, and demonstrated that most infants acquired oral bacteria from their biological mother even at the single-nucleotide level. Our results also indicated that the occupancies of maternal oral bacteria in infant oral microbiota were associated with the feeding methods of infants. These data could increase understanding of the early development of oral microbiota in infants and its potential associations with oral microbiota-related diseases..
5. Jiale Ma, Shinya Kageyama, Toru Takeshita, Yukie Shibata, Michiko Furuta, Mikari Asakawa, Yoshihisa Yamashita, Clinical utility of subgingival plaque-specific bacteria in salivary microbiota for detecting periodontitis., PloS one, 10.1371/journal.pone.0253502, 16, 6, e0253502, 2021.06, Saliva contains diverse bacteria shed from various oral sites, including subgingival plaque. It is reasonable to focus on the total occupancy of subgingival plaque-specific bacteria (SUBP bacteria), which live in subgingival environments, in the saliva for detecting periodontitis using salivary testing. This study aimed to validate the clinical utility of SUBP bacteria in the salivary microbiota for the detection of periodontitis. We examined stimulated saliva samples collected from 125 subjects who visited three dental clinics. The relative abundances of previously identified 11 SUBP bacteria were determined using 16S ribosomal RNA gene sequencing and a reference-based approach. The prediction performance was evaluated using a receiver operating characteristic (ROC) curve. The SUBP bacteria accounted for 0-15.4% of the salivary microbiota, and the percentage distinguished periodontitis patients with at least 15 sites with probing depth ≥4 mm with a sensitivity of 0.90 (95% confidence interval [CI], 0.81-0.98) and specificity of 0.70 (95% CI, 0.60-0.80) (area under the ROC curve [AUC], 0.87). Among 2,047 combinations of 11 SUBP bacteria, combinations including Streptococcus constellatus, Porphyromonas gingivalis, and Fusobacterium nucleatum subsp. vincentii demonstrated significantly higher AUC values in their detection. These results suggest that examining SUBP bacteria in saliva may be useful for detecting periodontitis patients in mass screening..
6. Shinya Kageyama, Yuka Nagao, Jiale Ma, Mikari Asakawa, Ryoji Yoshida, Toru Takeshita, Akiyuki Hirosue, Yoshihisa Yamashita, Hideki Nakayama, Compositional Shift of Oral Microbiota Following Surgical Resection of Tongue Cancer., Frontiers in cellular and infection microbiology, 10.3389/fcimb.2020.600884, 10, 600884-600884, 2020.11, Salivary microbiota is considered a source of microorganisms for the respiratory and digestive tracts, and a trigger for diseases in these distant organs. Meanwhile, the microbiota on the tongue surface is thought to be a major source of salivary microbiota. Therefore, surgical resection of the tongue for definitive treatment of oral cancer could drastically change the salivary bacterial balance and virulence. Here, we investigated the shift of the salivary microbiota following surgical resection in patients with tongue cancer. The stimulated saliva samples were collected from 25 tongue cancer patients pre- and post-resection of the tongue, and bacterial density and composition was determined using quantitative PCR analysis and 16S ribosomal RNA (rRNA) gene sequencing, respectively. Although no significant difference in the total bacterial density in saliva pre- and post-surgery was observed, the bacterial composition significantly differed according to the analysis of similarity. Among predominant operational taxonomic units (OTUs) with ≥1% of relative abundance, the proportions of OTUs corresponding to Streptococcus salivarius, Prevotellamelaninogenica, and Prevotellahisticola were significantly decreased following the tongue resection. On the other hand, the proportions of OTUs corresponding to Lautropiamirabilis, Neisseriaflava, Streptococcussanguinis, and Fusobacterium nucleatum, known to be inhabitants of dental plaque, were significantly increased. These results suggest that surgical resection of the tongue causes a compositional shift of the salivary microbiota, characterized by an increase in bacterial species derived from dental plaque, including periodontal pathogens. These results suggest the necessity of more careful and frequent postoperative oral care after surgical resection of tongue cancer..
1. 馬 佳楽, 影山 伸哉, 竹下 徹, 朝川 美加李, 山下 喜久, 16S rRNA遺伝子全長解析を用いた重度歯周病患者の口腔内各部位の細菌構成同定, 第72回日本口腔衛生学会・総会, 2023.05.
2. 馬 佳楽, 影山 伸哉, 竹下 徹, 朝川 美加李, 山下 喜久, 全長16S rRNA遺伝子配列解析を用いた重度歯周病患者の歯肉縁下プラークと舌苔の共有菌の解析, 第69回日本細菌学会・総会, 2023.03.
3. Jiale Ma, Michiko Furuta, Toru Takeshita, Shinya Kageyama, Mikari Asakawa, Shino Suma, Yoshihisa Yamashita, Fermented dairy food intake reduces risk of tooth loss in a Japanese community, The 69th Annual Meeting of Japanese Association for Dental Research, 2021.10.
4. Jiale Ma, Shinya Kageyama, Toru Takeshita, Yukie Shibata, Michiko Furuta, Mikari Asakawa, Yoshihisa Yamashita, Assessment of periodontitis by next-generation sequencing method focusing on subgingival plaque-specific bacteria in saliva, KOB & OBT Joint International Symposium 2021, 2021.02.
Membership in Academic Society
  • Japanese Association for Oral Biology
  • Japanese Society for Bacteriology
  • Japanese Society for Oral Health