Updated on 2025/04/25

Information

 

写真a

 
KAWAGUCHI YOSHIROU
 
Organization
Faculty of Engineering Department of Applied Chemistry Assistant Professor
Title
Assistant Professor
Contact information
メールアドレス
Tel
0928022808
Profile
経皮ワクチンに関する研究の推進、支援。 樹状細胞ターゲティングペプチドを用いた経皮ワクチンの高機能化検討。 指向性進化法に関する研究。
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Degree

  • Ph.D. Systems Life Sciences (Kyushu University)

Research History

  • 第一三共株式会社‐抗体および抗体様中分子を利用した創薬研究   

    第一三共株式会社‐抗体および抗体様中分子を利用した創薬研究

Research Interests・Research Keywords

  • Research theme： Creation of Dendritic Cell-Targeting Peptides for Transdermal Cancer Vaccines

    Keyword： Transdermal Vaccines, Peptides, Protein Engineering, Directed Evolution

    Research period： 2023.11 - 2026.3

Papers

  • Transdermal Insulin Delivery Using Ionic Liquid-Mediated Nanovesicles for Diabetes Treatment.

    Nabila FH, Islam R, Yamin L, Yoshirou K, Wakabayashi R, Kamiya N, Moniruzzaman M, Goto M

    ACS biomaterials science & engineering   11 ( 1 )   402 - 414   2025.1

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    Language：English  

    DOI： 10.1021/acsbiomaterials.4c02000

    PubMed

  • Ionic Liquid-Based Immunization Patch for the Transdermal Delivery of Antigens

    Islam, R; Nabila, FH; Wakabayashi, R; Kawaguchi, Y; Kamiya, N; Moniruzzaman, M; Goto, M

    MOLECULES   29 ( 13 )   2024.7   （ eISSN:1420-3049 ）

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    Language：English   Publisher：Molecules  

    Herein, we report a transdermal patch prepared using an ionic liquid-based solid in oil (IL-S/O) nanodispersion and a pressure-sensitive adhesive (PSA) to deliver the macromolecular antigenic protein, ovalbumin (OVA). The IL-S/O nanodispersion and a PSA were first mixed at an equal weight ratio, then coated onto a release liner, and covered with a support film. To evaluate the effect of the PSA, three types of PSAs, DURO-TAK 87-4098, DURO-TAK 87-4287, and DURO-TAK 87-235A, were used to obtain the corresponding IL-S/O patches SP-4098, SP-4287, and SP-235A, respectively. The prepared IL-S/O patches were characterized for surface morphology, viscoelasticity, and moisture content. In vitro skin penetration and in vivo immunization studies of the IL-S/O patches were performed using Yucatan micropig skin and the C57BL/6NJc1 mice model, respectively. The SP-4098 and SP-4287 delivered 5.49-fold and 5.47-fold higher amounts of drug compared with the aqueous formulation. Although both patches delivered a similar amount of drug, SP-4287 was not detached fully from the release liner after 30 days, indicating low stability. Mice immunized with the OVA-containing SP-4098 produced a 10-fold increase in anti-OVA IgG compared with those treated with an aqueous formulation. These findings suggested that the IL-S/O patch may be a good platform for the transdermal delivery of antigen molecules.

    DOI： 10.3390/molecules29132995

    Web of Science

    Scopus

    PubMed

  • A blocking monoclonal antibody reveals dimerization of intracellular domains of ALK2 associated with genetic disorders Reviewed International journal

    Takenobu Katagiri, Sho Tsukamoto, Mai Kuratani, Shinnosuke Tsuji, Kensuke Nakamura, Satoshi Ohte, Yoshiro Kawaguchi, Kiyosumi Takaishi

    Nature Communications   2023.5

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    Language：Japanese   Publishing type：Research paper (scientific journal)  

    Repository Public URL： https://hdl.handle.net/2324/7172165

  • Discovery of potent and specific inhibitors targeting the active site of MMP-9 from the engineered SPINK2 library Reviewed International journal

    Hidenori Yano, Daisuke Nishimiya, Yoshirou Kawaguchi, Masakazu Tamura, Ryuji Hashimoto

    PLoS One   2023.5

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    Language：Japanese   Publishing type：Research paper (scientific journal)  

    Repository Public URL： https://hdl.handle.net/2324/7172159

  • A protein scaffold, engineered SPINK2, for generation of inhibitors with high affinity and specificity against target proteases Reviewed International journal

    Daisuke Nishimiya, Yoshirou Kawaguchi, Shiho Kodama, Hatsumi Nasu, Hidenori Yano, Aya Yamaguchi, Masakazu Tamura, Ryuji Hashimoto

    Scientific Reports   2020.12

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    Language：Japanese   Publishing type：Research paper (scientific journal)  

    Repository Public URL： https://hdl.handle.net/2324/7171754

  • Generation of efficient mutants of endoglycosidase from Streptococcus pyogenes and their application in a novel one-pot transglycosylation reaction for antibody modification Reviewed International journal

    Mitsuhiro Iwamoto, Yukiko Sekiguchi, Kensuke Nakamura, Yoshirou Kawaguchi, Takeshi Honda, Jun Hasegawa

    PLoS One   2018.2

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    Language：Japanese   Publishing type：Research paper (scientific journal)  

    Repository Public URL： https://hdl.handle.net/2324/7171753

  • The crystal structure of novel chondroitin lyase ODV-E66, a baculovirus envelope protein Reviewed International journal

    Yoshirou Kawaguchi, Nobuo Sugiura, Koji Kimata, Makoto Kimura, Yoshimitu Kakuta

    FEBS Letters   2013.12

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    Language：Japanese   Publishing type：Research paper (scientific journal)  

    Repository Public URL： https://hdl.handle.net/2324/7172191

  • Crystal structure of human tyrosylprotein sulfotransferase-2 reveals the mechanism of protein tyrosine sulfation reaction Reviewed International journal

    Takamasa Teramoto, Yukari Fujikawa, Yoshirou Kawaguchi, Katsuhisa Kurogi, Masayuki Soejima, Rumi Adachi, Yuichi Nakanishi, Emi Mishiro-Sato, Ming-Cheh Liu, Yoichi Sakakibara, Masahito Suiko, Makoto Kimura, Yoshimitsu Kakuta

    Nature Communications   2013.6

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    Language：Japanese   Publishing type：Research paper (scientific journal)  

    Repository Public URL： https://hdl.handle.net/2324/7172241

  • Crystallization and X-ray diffraction analysis of chondroitin lyase from baculovirus: envelope protein ODV-E66 Reviewed International journal

    Yoshirou Kawaguchi, Nobuo Sugiura, Momo Onishi, Koji Kimata, Makoto Kimura, Yoshimitu Kakuta

    Acta Crystallographica Section F   2011.2

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    Language：Japanese   Publishing type：Research paper (scientific journal)  

    Repository Public URL： https://hdl.handle.net/2324/7172192

  • Glucuronyltransferase activity of KfiC from Escherichia coli strain K5 requires association of KfiA: KfiC and KfiA are essential enzymes for production of K5 polysaccharide, N-acetylheparosan Reviewed International journal

    Nobuo Sugiura, Yuichi Baba, Yoshirou Kawaguchi, Toru Iwatani, Kiyoshi Suzuki, Takahiro Kusakabe, Kiwamu Yamagishi, Koji Kimata, Yoshimitsu Kakuta, Hideto Watanabe

    The Journal of Biological Chemistry   2010.1

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    Language：Japanese   Publishing type：Research paper (scientific journal)  

    Repository Public URL： https://hdl.handle.net/2324/7172161

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Presentations

  • Crystal structure of human tyrosylprotein sulfotransferase International conference

    Yoshirou Kawaguchi, Takamasa Teramoto, Yukari Fujikawa, Katsuhisa Kurogi, Masayuki Soejima, Rumi Adachi, Yuichi Nakanishi, Emi Mishiro-Sato, Ming-Cheh Liu, Yoichi Sakakibara, Masahito Suiko, Makoto Kimura, Yoshimitsu Kakuta

    International Union of Crystallography  2011.8 

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    Event date： 2011.8

    Language：English  

    Country：Spain  

    Other Link： http://iucr2011.iucr.org/

  • 新規融合タンパク質による修飾位置を制御可能な抗体修飾法の開発

    西岡 莉子, 室園 晃輝, 内田 和希, 川口 喜郎, 木村 道夫, 神谷 典穂

    日本生物工学会大会講演要旨集  2024.8  (公社)日本生物工学会

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    Language：Japanese  

  • 近接効果を利用した架橋酵素によるタグフリーでの抗体フラグメント修飾

    室園 晃輝, 西岡 莉子, 内田 和希, 川口 喜郎, 木村 道夫, 神谷 典穂

    日本生物工学会大会講演要旨集  2024.8  (公社)日本生物工学会

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    Language：Japanese  

Industrial property rights

Patent   Number of applications: 0   Number of registrations: 2
Utility model   Number of applications: 0   Number of registrations: 0
Design   Number of applications: 0   Number of registrations: 0
Trademark   Number of applications: 0   Number of registrations: 0

Research Projects

  • Creation of non-invasive vaccines based on nanocoating technology of biomolecule

    Grant number：24H00397  2024.4 - 2027.3

    Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (A)

    後藤 雅宏, 田原 義朗, 神谷 典穂, 若林 里衣, 川口 喜郎

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    Grant type：Scientific research funding

    本研究では、ワクチン抗原に油状ナノコーティング技術（Solid-in-Oil（S/O）技術）を応用し、今まで注射でしか投与できなかったワクチンを塗り薬として投与できる“塗布型ワクチン”を創成する。
    申請者は現在までに、独自に開発したS/O技術を利用し、生体分子の皮膚浸透性の向上および免疫付与に成功した。本研究では、これまで誰も成し遂げたことのない社会実装可能な高効率経皮免疫システムを構築し、予防医療のパラダイムシフトを起こし、日本の医療費を大幅に削減するとともに、人々のQOL向上へ貢献する。
    本研究の進展によって、バイオ医薬品の経皮デリバリーという新しい学術領域が拓かれると期待している。

    CiNii Research

  • バキュロウイルス由来新奇コンドロイチンリアーゼの立体構造解明と機能改変

    2013 - 2014

    Japan Society for the Promotion of Science  Research Fellowships for Young Scientists

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    Grant type：Joint research

Educational Activities

  • 学部3年次学生実験：遺伝子変異導入と変異体タンパク質の評価（応用化学実験第三、物質科学工学実験第三）

Class subject

  • 応用化学実験第三

    2024.10 - 2025.3   Second semester

FD Participation

  • 2024.4   Role：Participation   Title：令和６年度 第１回全学FD（新任教員の研修）The 1st All-University FD (training for new faculty members) in FY2024

    Organizer：University-wide