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Authorship：Lead author,　Corresponding author   Language：English   Publishing type：Research paper (international conference proceedings)   Publisher：Society of Rheology  

The deformability of human red blood cells (RBCs), which comprise almost 99% of the cells in whole blood, is largely related not only to pathophysiological blood flow but also to the levels of intracellular compounds. Therefore, statistical estimates of the deformability of individual RBCs are of paramount importance in the clinical diagnosis of blood diseases. Although the microscale hydrodynamic interactions of individual RBCs lead to non-Newtonian blood rheology, there is no established method to estimate individual RBC deformability from the rheological data of RBC suspensions, and the possibility of this estimation has not been proven. To address this issue, we conducted an integrated analysis of a model of the rheology of RBC suspensions, coupled with macrorheological data of human RBCs suspended in plasma. Assuming a nonlinear curve of the relative viscosity of the suspensions as a function of the cell volume fraction, the statistical average of the membrane shear elasticity was estimated for individual intact RBCs or hardened RBCs. Both estimated values reproduced well the experimentally observed shear-thinning non-Newtonian behavior in these suspensions. We hereby conclude that our complementary approach makes it possible to estimate the statistical average of individual RBC deformability from macrorheological data obtained with usual rheometric tests.

DOI： 10.1122/8.0000877

Web of Science

Scopus

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Language：English   Publishing type：Research paper (scientific journal)   Publisher：Elsevier BV  

DOI： 10.1016/j.stem.2024.06.012

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Authorship：Lead author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Elsevier BV  

DOI： 10.1016/j.rineng.2024.102304

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Authorship：Lead author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：AIP Publishing  

We present a numerical analysis of the rheology of a suspension of red blood cells (RBCs) for different volume fractions in a wall-bounded, effectively inertialess, small amplitude oscillatory shear (SAOS) flow for a wide range of applied frequencies. The RBCs are modeled as biconcave capsules, whose membrane is an isotropic and hyperelastic material following the Skalak constitutive law. The frequency-dependent viscoelasticity in the bulk suspension is quantified by the complex viscosity, defined by the amplitude of the particle shear stress and the phase difference between the stress and shear. SAOS flow basically impedes the deformation of individual RBCs as well as the magnitude of fluid-membrane interactions, resulting in a lower specific viscosity and first and second normal stress differences than in steady shear flow. Although it is known that the RBC deformation alone is sufficient to give rise to shear-thinning, our results show that the complex viscosity weakly depends on the frequency-modulated deformations or orientations of individual RBCs but rather depends on combinations of the frequency-dependent amplitude and phase difference. The effect of the viscosity ratio between the cytoplasm and plasma and of the capillary number is also assessed.

DOI： 10.1063/5.0196272

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Authorship：Lead author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：American Physical Society (APS)  

DOI： 10.1103/physrevfluids.8.l061101

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Other Link： http://harvest.aps.org/v2/journals/articles/10.1103/PhysRevFluids.8.L061101/fulltext

Total pages：3 ,9, 475, 10p, 図版39p   Language：Japanese  

CiNii Books

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Event date： 2024.9

Language：Japanese   Presentation type：Oral presentation (keynote)  

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Event date： 2023.7

Language：English   Presentation type：Oral presentation (invited, special)  

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Language：Japanese  

Language：Japanese  

Language：Japanese  

Language：English   Publishing type：Article, review, commentary, editorial, etc. (scientific journal)   Publisher：AIP Publishing  

Estimation method allows red blood cell deformability to be determined from the viscosity of blood.

DOI： 10.1063/10.0030396

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Authorship：Lead author   Language：Japanese   Publishing type：Article, review, commentary, editorial, etc. (scientific journal)  

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J-GLOBAL

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J-GLOBAL

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Language：English   Publishing type：Article, review, commentary, editorial, etc. (scientific journal)   Publisher：MDPI AG  

Microfluidics is a powerful tool to precisely control fluids as well as to manipulate suspended small particles in a micrometer-sized space [...]

DOI： 10.3390/mi13071127

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Type：Seminar, workshop

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