https://kyushu-u.elsevierpure.com/en/persons/takeshi-uchiumi
　Reseacher Profiling Tool　Kyushu University Pure

092-642-6712

092-642-6712

Medical Doctor　, Doctor of Philosophy

No

Biochemistry, Molecular Biology、Clinical chemistry、Clinical Chemistry and Laboratory Medicine

02years00months

The Department of Health Science Laboratory Technology is working to develop the infrastructure of clinical chemistry and new measurement methods. In addition, mitochondrial research is being conducted as basic research. Mitochondrial DNA encodes the electron-transfer subunit that synthesizes ATP and is essential for cells to exist and function normally. Mitochondria are the largest site of reactive oxygen species generation in the cell, and mitochondrial DNA there is subject to very strong oxidative damage, and the concept that mitochondrial DNA mutations play an important role in the development and metastasis of diseases such as cancer, as well as in individual aging, is accepted. Functional analysis of genes involved in mitochondrial DNA replication, transcription, and translation is underway. Specifically, we are focusing on the mitochondrial ribosome-binding ERAL1 gene, which regulates translation, and the RNA-binding LRP130 gene. Functional analysis includes measurement of mitochondrial function (membrane potential, generation of reactive oxygen species, cell survival, and cell cycle) when gene expression is decreased by siRNA introduction, and functional evaluation (2) The CSD (cold shock domain) family of proteins has a nucleic acid binding domain consisting of approximately 78 amino acids that is highly conserved from E. coli to humans. CSD family proteins are ubiquitous proteins with a nucleic acid-binding domain consisting of about 78 amino acids that are highly conserved from E. coli to humans, and are expressed in many proliferating cells. In this study, (1) YB-1 knockout mice were generated and their functions were elucidated. Homozygous YB-1 knockout mice were embryonic lethal. (2) YB-1 was expressed almost throughout the mouse fetus by in situ hybridization. (3) YB-1 binds to the outer mitochondrial membrane and is involved in translation. (4) When YB-1 knockout fibroblasts were generated, cell proliferation was also decreased in experiments using YB-1 siRNA, confirming that the expression of EF-1 was upregulated upon YB-1 siRNA transfection. This suggests that YB-1 is deeply involved in cell proliferation, suggesting that YB-1 affects the expression of various proteins at the translational level. Furthermore, (5) YB1 was found to harbor a variety of microRNAs. This suggests that YB1 may be a potential molecular target in cancer, which could serve as a basis for new drug design. (6) Functional analysis of the mitochondrial matrix protein p32: From the individual level to the molecular level, we will examine how the mitochondria of intracellular organelles affect cells and individuals (7) Elucidation of new molecular mechanisms inferred from analysis of tissue-specific knockout mice of p32

Research Interests

• Physiological function analysis of mitochondrial protein ABCB10
  keyword : mitochondria transporter
  2022.06～2025.06.
• New molecular mechnism of the functional interaction between mitochondria and lysosome
  keyword : mitochondria, lysosome, NAD, functional interaction
  2020.06～2026.06.
• New approch for measurement of clinical chemistry
  
  keyword : Clinical chemistry
  2019.05～2022.05.
• Mitochondria and cancer
  keyword : mitochondria
  2016.05～2016.05.
• Functional analysis of mitochondrial protein p32
  keyword : p32 , mitochondria , translation
  2010.05～2018.05.
• Cross talk between cytoplasm and mitochndria translation
  keyword : mitochondria translation
  2008.05～2012.05.
• Mitochondria related gene and Cancer
  keyword : Mitochondria , tumor, translation, replication, siRNA
  2007.06～2010.05.
• Multifunction of YB-1 gene response to stress- cross-talk to translation, transcription and repair
  keyword : YB-1, translation, transcription, repair
  1998.04～2008.03.

Academic Activities
Membership in Academic Society

• American Association of Cancer Research
• Japanease Cancer Association

Educational Activities