Kyushu University [Hidefumi Maeda (Professor) Faculty of Dental Science, Department of Dental Science]

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Researcher information

Hidefumi Maeda
Professor
Oral Rehabilitation
Department of Dental Science
Faculty of Dental Science
Last modified date：2024.04.16

Graduate School

Department of Dental Science
Graduate School of Dental Science

Homepage

Academic Degree

Country of degree conferring institution (Overseas)

Field of Specialization

Total Priod of education and research career in the foreign country

Research

Research Interests

Development of the bio-implant with the ability to induce the formation of periodontal ligament
keyword : bio-implant, periodontal ligament
2022.04.
To elucidate the mechanism of aging alteration of dentin/pulp complex targeting oxidative stress
keyword : aging alteration, dentin/pulp complex, oxidative stress
2022.04.
Development of phage therapy for tooth root caries.
keyword : Phage therapy, Tooth root caries
2021.04～2024.03.
Development of a novel rejin adhesive including nanohaydroxyapatite as a direct pulp capping agent
keyword : nanohydroxyapatite, resin, direct pulp capping agent
2020.04.
Development of artificial periodontal ligament using 3D printer.
keyword : Artificial periodonatl ligament, 3D printer
2017.04.
Analysis of the mechanism of maintaining the stemness of periodontal ligament stem cells
keyword : priodontal ligament, stemness
2003.04.
Development of novel dental pulp capping materials
keyword : direct pulp capping
2015.04～2022.03.
Analysis of the mechanism of senescence of dental pulp cells.
keyword : dental pulp cells, senescence, aging
2014.04.
Development of the artificial tooth root with periodontal ligament tissue.
keyword : artificial tooth root, periodontal ligament tissue
2017.04.
Development of periodontal ligament stem cells from human iPS cells.
keyword : periodontal ligament stem cells, iPS cells
2017.04.
Development of regenerative therapy of periodontal tissue using iPS cells.
keyword : iPS cells
2012.04.
Development of bioactive resin with the potency of regeneration of periodontal ligament tissue
keyword : Periodontal ligament tissue, Bioactive resin
2013.05～2017.03.
Development of regenerative therapy of periodontal tissue
keyword : periodontal stem cell
2003.04.
Development of immortalized human periodontal ligament cells, cloning of them, and detection of gene specific for these cells
keyword : periodontal cell
2003.04～2006.03The establishment of immortal cell line of human periodontal cells, their cloning, and the detection of the gene specific for them.
Evaluation of the effects of human periapical granulation tissue on bone metabolism
keyword : periapical granuloma, osteoblast
1998.04～1999.03The elucidation about whether fibroblasts derived from humanperiapical granulation tissue have the potentials to calcify..

Current and Past Project

Mechanism of Transforming Growth Factor β1 to Promotes Migration of Human Periodontal Ligament Cells
Mineral Trioxide Aggregate (MTA) induces the osteoblastic/cementoblastic differentiation of human PDL cells to activate mineralization. We aim to disclose the mechanism of bioactive effects of MTA, and to develope the new therapy for the regeneration of human periodontal ligament.

Academic Activities

Books

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1.	Hidefumi Maeda, ‘Mass acquisition of human periodontal ligament stem cells’ Editor: Gul Ozcan ‘Recent Research Advances in Biology’, B.P. International, 10.9734/bpi/rrab/v9/2682F, pp123-133, 2021.09.
2.	Naohisa Wada, Atsushi Tomokiyo, Hidefumi Maeda, Future Perspectives in Dental Stem Cell Engineering and the Ethical Considerations. , Springer, Dental Stem Cells. pp 289-307, 2016.06.
3.	Atsushi Tomokiyo, Naohisa Wada, Hidefumi Maeda, Contribution of Stem Cells to Dental Tissue Regeneration: Isolation, Function, and Application., Frontiiers, Frontiers in Stem Cell and Regenerative Medicine Research, Vol. 2, pp3-38, 2016.12.
4.	Hidefumi Maeda, naohisa wada, Atsushi Tomokiyo, Monnouchi Satoshi, Akifumi Akamine, Prospective Potency of TGF-1 on Maintenance and Regeneration of Periodontal Tissue, Elsevier Academic Press, 283-367, 2013.06.
5.	Maeda H, Tomokiyo A, Wada N, Akamine A, INDUCTION OF BMP-2 IN PERIODONTAL LIGAMENT CELLS BY CALCIUM-BASED BIOMATERIAL. In: Anja Nohe (ed) Bone Morphogenetic Proteins: New Research., Nova Science Publishers, Inc., pp187-202., 2012.02.
6.	Hidefumi Maeda, Shinsuke Fujii, Satoshi Monnouchi, Naohisa Wada and Akifumi Akamine , Differentiation of Periodontal Stem/Progenitor Cells: Roles of TGF-beta1 In: M.A. Hayat (ed) Stem Cells and Cancer Stem Cells : Therapeutic Applications in Disease and Injury, Volume 4., Springer, pp51-58, 2012.05.
7.	Hidefumi Maeda, Naohisa Wada, Shinsuke Fujii, Atsushi Tomokiyo & Akifumi Akamine, Periodontal ligament stem cells, InTech, In Press, 2011.08.

Reports

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1.	Hamano S, Sugiura R, Yamashita D, Tomokiyo A, Hasegawa D, Maeda H., Current application of iPS cells in the dental tissue regeneration., Biomedicines, 10.3390/biomedicines10123269, 2022.12.
2.	Maeda H, Aging and senescence of dental pulp and hard tissues of the tooth., Front Cell Dev Biol., 10.3389/fcell.2020.605996, 2020.11.
3.	Maeda H, Mass acquisition of human periodontal ligament stem cells., World J Stem Cells., 10.4252/wjsc.v12.i9.1023, 2020.09, The periodontal ligament (PDL) is an essential fibrous tissue for tooth retention in the alveolar bone socket. PDL tissue further functions to cushion occlusal force, maintain alveolar bone height, allow orthodontic tooth movement, and connect tooth roots with bone. Severe periodontitis, deep caries, and trauma cause irreversible damage to this tissue, eventually leading to tooth loss through the destruction of tooth retention. Many patients suffer from these diseases worldwide, and its prevalence increases with age. To address this issue, regenerative medicine for damaged PDL tissue as well as the surrounding tissues has been extensively investigated regarding the potential and effectiveness of stem cells, scaffolds, and cytokines as well as their combined applications. In particular, PDL stem cells (PDLSCs) have been well studied. In this review, I discuss comprehensive studies on PDLSCs performed in vivo and contemporary reports focusing on the acquisition of large numbers of PDLSCs for therapeutic applications because of the very small number of PDLSCs available in vivo. .
4.	Yoshida S, Tomokiyo A, Hasegawa D, Hamano S, Sugii H, Maeda H., Insight into the Role of Dental Pulp Stem Cells in Regenerative Therapy., Biology (Basel), 10.3390/biology9070160., 2020.07.
5.	＠Hosoya N, ＠Takigawa T, ＠Horie T, Maeda H, ＠Yamamoto Y, ＠Momoi Y, ＠Yamamoto K, ＠Okiji T., A review of the literature on the efficacy of mineral trioxide aggregate in conservative dentistry., Dent Mater J., 2019.10.
6.	Tomokiyo A, Wada N, Maeda H., Periodontal Ligament Stem Cells: Regenerative Potency in Periodontium. , Stem Cells Dev., 2019.08.
7.	Tomokiyo A, Yoshida S, Hamano S, Hasegawa D, Sugii H, Maeda H., Detection, Characterization, and Clinical Application of Mesenchymal Stem Cells in Periodontal Ligament Tissue., Stem Cells Int., 2018.08.
8.	Tomokiyo A, Hamano S, Hasegawa D, Sugii S, Yoshida S, Maeda H. , Prospects for the Application of Neural Crest Cells for the Periodontal Therapy., J Dent Oral Biol. , 2017.09.
9.	Atsushi Tomokiyo, Naohisa Wada, Hidefumi Maeda, Contribution of stem cells to dental tissue regeneration; isolation, function, and application., Frontiers in Stem Cell and Regenerative Medicine Research Vol. 2, 2016, 3-38, 2016.07.
10.	Naohisa Wada, Atsushi Tomokiyo, Hidefumi Maeda, Future Perspectives in Dental Stem Cell Engineering and the Ethical Considerations. , Dental Stem Cells. , 2016.06.
11.	Hidefumi Maeda, Atsushi Tomokiyo, naohisa wada, Koori Katsuaki, Giichiro Kawachi, Akifumi Akamine, Regeneration of the periodontium for preservation of the damaged tooth, Hindawi Publishing Corporation, 2014.10, The population of the world grows every year, and life expectancy tends to increase. Thus, longterm preservation of teeth in aged individuals is an urgent issue. The main causes of tooth loss are well known to be periodontitis, caries, fractures, and orthodontic conditions. Although implant placement is a widely accepted treatment for tooth loss, most patients desire to preserve their own teeth. Many clinicians and researchers are therefore challenged to treat and preserve teeth that are irreversibly affected by deep caries, periodontitis, fractures, and trauma. Tissue engineering techniques are beneficial in addressing this issue; stem cells, signal molecules, and scaffolds are the main elements of such techniques. In this review, we describe these three elements with respect to their validation for regeneration of the periodontium and focus particularly on the potency of diverse scaffolds. In addition, we provide a short overview of the ongoing studies of 4-methacryloxyethyl trimellitate anhydride/methyl methacrylate-tri-n-butyl-borane resin including calcium chloride or hydroxyapatite for periodontium regeneration..
12.	Hidefumi Maeda, Akifumi Akamine, Quest for the development of tooth root/periodontal ligament complex by tissue engineering. , Integr Mol Med. , 1(2): 22-25, 2014. Doi: 10.15761/IMM.1000106, 2014.10, The life-span of the tooth is intimately-associated with healthiness of periodontal ligament (PDL) which is a connective tissue situated between bone and cementum that covers tooth root surface. However, once this tissue is severely damaged by deep caries, periodontitis, and trauma, this leads to severe difficulty in its regeneration, resulting in tooth loss and decreased quality of life. The development of the therapy for generation and regeneration of the periodontal tissue is an urgent issue. Therefore, researchers have tried to improve efficiently-generative and regenerative medicine using stem cells, signal molecules, and scaffolds, requisite for tissue regeneration. In recent studies, a dental follicle tissue that is composed of stem cell population potentially differentiating into PDL tissue, cementum, and alveolar bone is of current interest. More recently a revolutionary and attractive study reporting the development of bio-hybrid implant that reserved newly-formed cementum/PDL tissue complex on its surface was introduced. In this review, we describe comprehensive reports that tried to develop the cementum/PDL complex by tissue engineering and future prospects..
13.	Hidefumi Maeda, naohisa wada, Atsushi Tomokiyo, Monnouchi Satoshi, Akifumi Akamine, Prospective Potency of TGF-1 on Maintenance and Regeneration of Periodontal Tissue., Elsevier Adadmic Press, 2013.06.
14.	Hidefumi Maeda, Shinsuke Fujii, Atsushi Tomokiyo, Naohisa Wada and Akifumi Akamine, Potentials of periodontal ligament stem/progenitor cell lines in regeneration studies, Quintessence, 2011.12.
15.	Hidefumi Maeda, Atsushi Tomokiyo, Shinsuke Fujii, Naohisa Wada and Akifumi Akamine, Promise of periodontal ligament stem cells, BioMed Central, 2011.07.

Papers

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1.	Hamano S, Yamashita D, Hasegawa D, Sugii H, Itoyama T, Maeda H., Effect of fibrillin-2 on differentiation into periodontal ligament stem cell-like cells derived from human-induced pluripotent stem cells. , Stem Cells Dev, 10.1089/scd.2024.0013, 2024.03.
2.	Alhasan MA, Tomokiyo A, Hamano S, Sugii H, Ono T, Ipposhi K, Yamashita K, Mardini B, Minowa F, Maeda H., Hyaluronic Acid Induction Promotes the Differentiation of Human Neural Crest-like Cells into Periodontal Ligament Stem-like Cells, Cells, 10.3390/cells12232743, 12, 2743, 2023.11.
3.	Fujino S, Hamano S, Tomokiyo A, Sugiura R, Yamashita D, Hasegawa D, Sugii H, Fujii S, Itoyama T, Miyaji H, Maeda H., Dopamine is involved in reparative dentin formation through odontoblastic differentiation of dental pulp stem cells., Sci Rep, 10.1038/s41598-023-32126-1., 2023.04.
4.	Hamano S, Sugiura R, Yamashita D, Tomokiyo A, Hasegawa D, Maeda H. , Current application of iPS cells in the dental tissue regeneration, Biomedicines, 10.3390/biomedicines10123269, 13, 5668, 2022.12.
5.	Adachi O, Sugii H, Itoyama T, Fujino S, Kaneko H, Tomokiyo A, Hamano S, Hasegawa D, Obata J, Yoshida S, Kadowaki M, Sugiura R, Albougha S, Maeda M. , Decorin promotes osteoblastic differentiation of human periodontal ligament stem cells., Molecules., 10.3390/molecules27238224, 2022.11.
6.	Kadowaki M, Yoshida S, Itoyama T, Tomokiyo A, Hamano S, Hasegawa D, Sugii H, Kaneko H, Sugiura R, Maeda H., Involvement of M1/M2 macrophage polarization in reparative dentin formation., Life (Basel), 2022.11.
7.	Sugiura R, Hamano S, Tomokiyo A, Hasegawa D, Yoshida S, Sugii H, Fujino S, Adachi O, Kadowaki M, Yamashita D, Maeda H., PAX9 regulates periodontal ligament stem cell-like differentiation of human induced pluripotent stem cells. , Biomedicines. , 10.3390/biomedicines10102366, 2022.09.
8.	Nagano R, Fujii S, Hasegawa K, Maeda H, Kiyoshima T., Wnt signaling promotes tooth germ development through YAP1-TGF-β signaling., Biochem Biophys Res Commun., 10.1016/j.bbrc.2022.09.012, 2022.11.
9.	Fakatava N, Mitarai H, Yuda A, Haraguchi A, Wada H, Hasegawa D, Maeda H, Wada N., Actin alpha 2, smooth muscle, a transforming growth factor-β1-induced factor, regulates collagen production in human periodontal ligament cells via Smad2/3 pathway., J Dent Sci., 10.1016/j.jds.2022.08.030, 18, 2, 567-576, 2023.04.
10.	Kaneko H, Hasegawa D, Itoyama T, Yoshida S, Tomokiyo A, Hamano S, Sugii H, Maeda H., Inhibition of c-Jun N-terminal kinase signaling promotes osteoblastic differentiation of periodontal ligament stem cells and induces regeneration of periodontal tissues., Arch Oral Biol., 2022.02.
11.	Sugii H, Albougha MS, Adachi O, Tomita H, Tomokiyo A, Hamano S, Hasegawa D, Yoshida S, Itoyama T, Maeda H., Activin A promotes osteoblastic differentiation of human pre-osteoblasts through the ALK1-Smad1/5/9 pathway., Int J Mol Sci. , 2021.12.
12.	Yoshida S, Sugii H, Itoyama T, Kadowaki M, Hasegawa D, Tomokiyo A, Hamano S, Ipposhi K, Yamashita K, Maeda H., Development of a novel direct dental pulp-capping material using 4-META/MMA-TBB resin with nano hydroxyapatite., Mater Sci Eng C Mater Biol Appl., 10.1016/j.msec.2021.112426, 2021.11.
13.	Yamashita K, Tomokiyo A, Ono T, Ipposhi K, Alhasan MA, Tsuchiya A, Hamano S, Sigii H, Yoshida S, Itoyama T, Maeda H., Mineral trioxide aggregate immersed in sodium hypochlorite reduce the osteoblastic differentiation of human periodontal ligament stem cells., Sci Rep, 2021.11, White mineral trioxide aggregate (WMTA) is a root canal treatment material, which is known to exhibit a dark brown color when in contact with sodium hypochlorite solution (NaOCl). This study aimed to investigate the effects of NaOCl on the surface properties of WMTA discs and WMTA-induced osteoblastic differentiation of periodontal ligament stem cells (PDLSCs). Mixed WMTA (ProRoot MTA) was filled into the molds to form WMTA discs. These discs were immersed in distilled water (D-WMTA) or 5% NaOCl (Na-WMTA). Their surface structures and Ca2+ release level was investigated. Moreover, they were cultured with a clonal human PDLSC line (line 1–17 cells). The main crystal structures of Na-WMTA were identical to the structures of D-WMTA. Globular aggregates with polygonal and needle-like crystals were found on D-WMTA and Na-WMTA, which included Ca, Si, Al, C and O. However, many amorphous structures were also identified on Na-WMTA. These structures consisted of Na and Cl, but did not include Ca. NaOCl immersion also reduced Ca2+ release level from whole WMTA discs. Line 1–17 cells cultured with D-WMTA formed many mineralized nodules and exhibited high expression levels of osteoblast-related genes. However, cells incubated with Na-WMTA generated a small number of nodules and showed low expression levels of osteoblast-related genes. These results indicated that NaOCl reduced Ca2+ release from WMTA by generating amorphous structures and changing its elemental distribution. NaOCl may also partially abolish the ability of WMTA to stimulate osteoblastic differentiation of PDLSCs..
14.	Ipposhi K, Tomokiyo A, Ono T, Yamashita K, Alhasan MA, Hasegawa D, Hamano S, Yoshida S, Sugii H, Itoyama T, Ogawa M, Maeda H., Secreted frizzled-related protein 1 promotes odontoblastic differentiation and reparative dentin formation by regulating Notch signaling in dental pulp cells., Cells, 10.3390/cells10092491, 10, 9, 2021.09.
15.	Tomokiyo A, Hasegawa D, Ono T, Nagano R, Ipposhi K, Yamashita K, Alhasan MA, Maeda H., Characterization of a clonal human periodontal ligament stem cell line exposed to methacrylate resin-, bioactive glass-, or silicon-based root canal sealers., Odontology, 10.1007/s10266-021-00648-7, 2021.08.
16.	Ono T, Tomokiyo A, Ipposhi K, Yamashita K, Alhasan MA, Miyazaki Y, Kunitomi Y, Tsuchiya A, Ishikawa K, Maeda H., Generation of biohybrid implants using a multipotent human periodontal ligament cell line and bioactive core materials., J Cell Physiol, 10.1002/jcp.30336, 2021.06.
17.	Maeda H. , Aging and senescence of dental pulp and hard tissues of the tooth., Front Cell Dev Biol., 10.3389/fcell.2020.605996, 2020.11.
18.	Maeda H, Mass acquisition of human periodontal ligament stem cells., World J Stem Cells, 10.4252/wjsc.v12.i9.1023, 12, 9, 1023-1031, 2020.09, The periodontal ligament (PDL) is an essential fibrous tissue for tooth retention in the alveolar bone socket. PDL tissue further functions to cushion occlusal force, maintain alveolar bone height, allow orthodontic tooth movement, and connect tooth roots with bone. Severe periodontitis, deep caries, and trauma cause irreversible damage to this tissue, eventually leading to tooth loss through the destruction of tooth retention. Many patients suffer from these diseases worldwide, and its prevalence increases with age. To address this issue, regenerative medicine for damaged PDL tissue as well as the surrounding tissues has been extensively investigated regarding the potential and effectiveness of stem cells, scaffolds, and cytokines as well as their combined applications. In particular, PDL stem cells (PDLSCs) have been well studied. In this review, I discuss comprehensive studies on PDLSCs performed in vivo and contemporary reports focusing on the acquisition of large numbers of PDLSCs for therapeutic applications because of the very small number of PDLSCs available in vivo..
19.	Hasegawa D, Hasegawa K, Kaneko H, Yoshida S, Mitarai H, Arima M, Tomokiyo A, Hamano S, Sugii H, Wada N, Kiyoshima H, Maeda H., MEST Regulates the Stemness of Human Periodontal Ligament Stem Cells., Stem Cells Int., 10.1155/2020/9672673, 2020.07.
20.	Yoshida S, Tomokiyo A, Hasegawa D, Hamano S, Sugii H, Maeda H., Insight into the Role of Dental Pulp Stem Cells in Regenerative Therapy., Biology-Basel., 10.3390/biology9070160, 9, 7, 160, 2020.07.
21.	Itoyama T, Yoshida S, Tomokiyo A, Hasegawa D, Hamano S, Sugii H, Ono T, Fujino S, Maeda H., Possible function of GDNF and Schwann cells in wound healing of periodontal tissue., J Periodont Res, 10.1111/jre.12774., 2020.06.
22.	Hamano S, Tomokiyo A, Hasegawa D, Yuda A, Sugii H, Yoshida S, Mitarai H, Wada N, Maeda H., Functions of beta2-adrenergic receptor in human periodontal ligament cells., J Cell Biochem., 10.1002/jcb.29706., 2020.04.
23.	藤野翔香、濱野さゆり、糸山知宏、前田英史．, 象牙芽細胞分化におけるドーパミンの発現．, 日歯内療誌, 10.1002/jcp.29314., 2020.01.
24.	Arima M, Hasegawa D, Yoshida S, Mitarai H, Tomokiyo A, Hamano S, Sugii H, Wada N, Maeda H., R-spondin 2 promotes osteoblastic differentiation of immature human periodontal ligament cells through the Wnt/-catenin signaling pathway., J Periodont Res., 10.1111/jre.12611, 54, 2, 143-153, 2019.02.
25.	Nozu A, Hamano S, Tomokiyo A, Hasegawa D, Sugii H, Yoshida S, Mitarai H, Taniguchi S, Wada N, Maeda H., Senescence and odontoblastic differentiation of dental pulp cells., J Cell Physiol, 10.1002/jcp.26905, 234, 1, 849-859, ??, 2019.01, Cellular senescence has been suggested to be involved in physiological changes of cytokine production. Previous studies showed that the concentration of tumor necrosis factor‐α (TNF‐α) is higher in the blood of aged people compared with that of young people. So far, the precise effects of TNF‐α on the odontoblastic differentiation of pulp cells have been controversial. Therefore, we aimed to clarify how this cytokine affected pulp cells during aging. Human dental pulp cells (HDPCs) were cultured until reaching the plateau of their growth, and the cells were isolated at actively (young HDPCs; yHDPCs) or inactively (senescent HDPCs; sHDPCs) proliferating stages. sHDPCs expressed senescence‐related molecules while yHDPCs did not. When these HDPCs were cultured in an odontoblastinductive medium, both young and senescent cells showed mineralization, but mineralization in sHDPCs was lower compared with yHDPCs. However, the administration of TNF‐α to this culture medium altered these responses: yHDPCs showed downregulated mineralization, while sHDPCs exhibited significantly increased mineralization. Furthermore, the expression of tumor necrosis factor receptor 1 (TNFR1), a receptor of TNF‐α, was significantly upregulated in sHDPCs compared with yHDPCs. Downregulation of TNFR1 expression led to decreased mineralization of TNF‐α‐treated sHDPCs, whereas restored the reduction in TNF‐α‐treated yHDPCs. These results suggested that sHDPCs preserved the odontoblastic differentiation capacity and TNF‐α promoted odontoblastic differentiation of HDPCs with the progress of their population doublings through increased expression of TNFR1. Thus, TNF‐α might exert a different effect on the odontoblastic differentiation of HDPCs depending on their proliferating activity. In addition, the calcification of pulp chamber with age may be related with increased reactivity of pulp cells to TNF‐α..
26.	Hiromi Mitarai, Naohisa Wada, Daigaku Hasegawa, Shinichiro Yoshida, Mai Arima, Atsushi Tomokiyo, Sayuri Hamano, Suguru Serita, Hiroyuki Mizumachi, Hidefumi Maeda, Transgelin mediates TGF-β1-induced proliferation of human periodontal ligament cells., J Peirodont Res, 2017.06.
27.	Shinichiro Yoshida, Naohisa Wada, Daigaku Hasegawa, Miyaji, Hiromi Mitarai, Atsushi Tomokiyo, Sayuri Hamano, Hidefumi Maeda, Semaphorin 3A Induces Odontoblastic Phenotype in Dental Pulp Stem Cells. , J Dent Res., 10.1177/0022034516653085, 95, 11, 1282-1290, 2016.09, In cases of pulp exposure due to deep dental caries or severe traumatic injuries, existing pulp-capping materials have a limited ability to reconstruct dentin-pulp complexes and can result in pulpectomy because of their low potentials to accelerate dental pulp cell activities, such as migration, proliferation, and differentiation. Therefore, the development of more effective therapeutic agents has been anticipated for direct pulp capping. Dental pulp tissues are enriched with dental pulp stem cells (DPSCs). Here, the authors investigated the effects of semaphorin 3A (Sema3A) on various functions of human DPSCs in vitro and reparative dentin formation in vivo in a rat dental pulp exposure model. Immunofluorescence staining revealed expression of Sema3A and its receptor Nrp1 (neuropilin 1) in rat dental pulp tissue and human DPSC clones. Sema3A induced cell migration, chemotaxis, proliferation, and odontoblastic differentiation of DPSC clones. In addition, Sema3A treatment of DPSC clones increased β-catenin nuclear accumulation, upregulated expression of the FARP2 gene (FERM, RhoGEF, and pleckstrin domain protein 2), and activated Rac1 in DPSC clones. Furthermore, in the rat dental pulp exposure model, Sema3A promoted reparative dentin formation with dentin tubules and a well-aligned odontoblast-like cell layer at the dental pulp exposure site and with novel reparative dentin almost completely covering pulp tissue at 4 wk after direct pulp capping. These findings suggest that Sema3A could play an important role in dentin regeneration via canonical Wnt/β-catenin signaling. Sema3A might be an alternative agent for direct pulp capping, which requires further study..
28.	Myna N. Zakaria, Toru Takeshita, Yukie Shibata, Hidefumi Maeda, Naohisa Wada, Akifumi Akamine, Yoshihisa Yamashita, Microbial community in persistent apical periodontitis: a 16S rRNA gene clone library analysis., Int Endod J., 10.1111/iej.12361, 48, 8, 717-728, 2015.08, AIM: To characterize the microbial composition of persistent periapical lesions of root filled teeth using a molecular genetics approach. METHODOLOGY: Apical lesion samples were collected from 12 patients (23-80 years old) who visited the Kyushu University Hospital for apicectomy with persistent periapical lesions associated with root filled teeth. DNA was directly extracted from each sample and the microbial composition was comprehensively analysed using clone library analysis of the 16S rRNA gene. Enterococcus faecalis, Candida albicans and specific fimA genotypes of Porphyromonas gingivalis were confirmed using polymerase chain reaction (PCR) analysis with specific primers. RESULTS: Bacteria were detected in all samples, and the dominant findings were P. gingivalis (19.9%), Fusobacterium nucleatum (11.2%) and Propionibacterium acnes (9%). Bacterial diversity was greater in symptomatic lesions than in asymptomatic ones. In addition, the following bacteria or bacterial combinations were characteristic to symptomatic lesions: Prevotella spp., Treponema spp., Peptostreptococcaceae sp. HOT-113, Olsenella uli, Slackia exigua, Selemonas infelix, P. gingivalis with type IV fimA, and a combination of P. gingivalis, F. nucleatum, and Peptostreptococcaceae sp. HOT-113 and predominance of Streptococcus spp. On the other hand, neither Enterococcus faecalis nor C. albicans were detected in any of the samples. CONCLUSION: Whilst a diverse bacterial species were observed in the persistent apical lesions, some characteristic patterns of bacterial community were found in the symptomatic lesions. The diverse variation of community indicates that bacterial combinations as a community may cause persistent inflammation in periapical tissues rather than specific bacterial species..
29.	Hidefumi Maeda, Akifumi Akamine, Quest for the development of tooth root/periodontal ligament complex by tissue engineering., Integr Mol Med., 10.15761/IMM.1000106, 1, 2, 22-25, 2014.10, The life-span of the tooth is intimately-associated with healthiness of periodontal ligament (PDL) which is a connective tissue situated between bone and cementum that covers tooth root surface. However, once this tissue is severely damaged by deep caries, periodontitis, and trauma, this leads to severe difficulty in its regeneration, resulting in tooth loss and decreased quality of life. The development of the therapy for generation and regeneration of the periodontal tissue is an urgent issue. Therefore, researchers have tried to improve efficiently-generative and regenerative medicine using stem cells, signal molecules, and scaffolds, requisite for tissue regeneration. In recent studies, a dental follicle tissue that is composed of stem cell population potentially differentiating into PDL tissue, cementum, and alveolar bone is of current interest. More recently a revolutionary and attractive study reporting the development of bio-hybrid implant that reserved newly-formed cementum/ PDL tissue complex on its surface was introduced. In this review, we describe comprehensive reports that tried to develop the cementum/PDL complex by tissue engineering and future prospects..
30.	Biocompatibility of resin-based sealers－ Comparison of Superbond sealer with AH Plus －.
31.	The Effects of MTA on Human Periodontal Ligament Cells..
32.	SEM images of root canal dentin irrigated with EDTA and NaOCl　－ Comparison with ultrasonic irrigation －.
33.	Effects of root-end filling materials on the osteoblast-like differentiation of human periodontal ligament cells.
34.	Goto T, Maeda H, Tanaka T., A selective inhibitor of matrix metalloproteases inhibits the migration of isolated osteoclasts by increasing the life span of podosomes., Journal of Bone and Mineral Metabolism, 10.1007/s007740200013, 20, 2, 98-105, 20 (2): 98-105, 2002.01.
35.	Maeda H, Akasaki K, Yoshimine Y, Akamine A, and Yamamoto K., Limited and Selective Localization of the Lysosomal Membrane Glycoproteins LGP85 and LGP96 in Rat Osteoclasts., Histochemistry and Cell Biology, 10.1007/s004180050354, 111, 4, 245-251, 111 (4): 245-251, 1999.01.
36.	Kukita A, Kukita T, Ouchida M, Maeda H, Yatsuki H, and Kohashi O., Osteoclast-derived zinc finger (OCZF) protein with POZ domain, a possible transcriptional repressor, is involved in osteoclastogenesis., Blood, 94, 6, 1987-1997, 94 (6): 1987-1997, 1999.01.
37.	Tsukuba T, Sakai H, Yamada M, Maeda H, Hori H, Azuma T, Akamine A, and Yamamoto K., Biochemical Properties of the Monomeric Mutant of Human Cathepsin E Expressed in Chinese Hamster Ovary Cells: Comparison with Dimeric Forms of the Natural and Recombinant Cathepsin E., J Biochemistry, 119, 1, 126-134, 119 (1): 126-134, 1996.01.
38.	Kukita T, Kukita A, Nagata K, Maeda H, Kurisu K, Watanabe T, and Iijima T, Novel Cell-Surface Ag Expressed on Rat Osteoclasts Regulating the Function of the Calcitonin Receptor., J Immunology, 153, 11, 5265-5273, 153 (11): 5265-5273, 1995.01.

Presentations

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1.	Albougha MS, Sugii H, Tomokiyo A, Hamano S, Hasegawa D, Yoshida S, Obata J, Itoyama T, Kaneko H, Kadowaki M, Kinoshita K, Maeda H., Exosomes from human periodontal ligament stem cells promote bone healing., The 71st Annual Meeting of Japanese Association for Demtal Research., 2023.11.
2.	Sugii S, Yoshida S, Hamano S, Hasegawa D, Obata J, Itoyama T, Albougha MS, Minowa F, Tomokiyo A, Maeda H. , The 4-META/MMA-TBB Resin Containing Nano Hydroxyapatite Regenerates Wounded Periodontal Tissue At The Perforation Site., The 22nd Scientific Congress of Asian Pacific Endodontic Confederation, 2023.08.
3.	M Anas Alhasan, Atushi Tomokiyo, Taiga Ono, Keita Ippoushi, Kozue Yamashita, Sayuri Hamano, Daigaku Hasegawa, Hideki Sugii, Hidefumi Maeda., Hyaluronic Acid Promotes the Differentiation of Human Neural Crest-like Cells into Periodontal Ligament Stem-like Cells., 第157回日本歯科保存学会秋季学術大会, 2022.11.
4.	Naati Fakatava、御手洗裕美、祐田明香、原口晃、長谷川大学、前田英史、和田尚久．, ACTA2 regulates human PDL function via interaction with or without TGF-1., 第156回日本歯科保存学会春季学術大会, 2022.06.
5.	Mhd Safwan Albougha, Hideki Sugii, Atsushi Tomokiyo, Sayuri Hamano, Daigaku Hasegawa, Shinichiro Yoshida, Tomohiro Itoyama, Shoko Fujino, Taiga Ono, Orie Adachi, Hidefumi Maeda., Exosomes derived from human periodontal ligament stem cells promote osteoblastic differentiation of pre-osteoblasts., 第156回日本歯科保存学会春季学術大会, 2022.06.
6.	Kaneko H, Hasegawa D, Itoyama T, Yoshida S, Tomokiyo A, Hamano S, Sugii H, Maeda H., Inhibition of c-Jun N-terminal kinase affects regeneration of periodontal tissues, KOB & OBT 5th Joint International Symposium, 2021.11.
7.	Keita Ipposhi, Atsushi Tomokiyo, Taiga Ono, Kozue Yamashita, M. Anas Alhasan, Hirofumi Miyaji, Daigaku Hasegawa, Sayuri Hamano, Shinichiro Yoshida, Hideki Sugii, Tomohiro Itoyama, Marina Ogawa, Tsutomu Sugaya, Hidefumi Maeda. , Secreted frizzled-related protein 1 promotes odontoblastic differentiation and reparative dentin formation in dental pulp cells., The International Scientific Meeting of 30th Anniversary of Korean Academy of Endodontics & the 19th JEA-KAE Joint Scientific Meeting., 2021.10.
8.	Kozue Yamashita, Atsushi Tomokiyo, Taiga Ono, Keita Ipposhi, Sayuri Hamano, Hideki Sugii, Shinichiro Yoshida, Tomohiro Itoyama, Hidefumi Maeda. , Effects of sodium hypochlorite and iodine potassium iodide on features of mineral trioxide aggregate., The International Scientific Meeting of 30th Anniversary of Korean Academy of Endodontics & the 19th JEA-KAE Joint Scientific Meeting., 2021.10.
9.	Tomohiro Itoyama, Kenji Watanabe, Masataka Kadowaki, Hiroshi Kaneko, Shinichiro Yoshida, Takahiko Shimizu, Hidefumi Maeda., Superoxide dismutase 2 is involved in the senescence of dentin-pulp complex., The International Scientific Meeting of 30th Anniversary of Korean Academy of Endodontics & the 19th JEA-KAE Joint Scientific Meeting., 2021.10.
10.	Hideki Sugii, Mhd Safwan Albougha, Orie Adachi, Hiroka Tomita, Atsushi Tomokiyo, Sayuri Hamano, Daigaku Hasegawa, Shinichiro Yoshida, Junko Obata, Tomohiro Itoyama, Taiga Ono, Keita Ippoushi and Hidefumi Maeda., Activin A mediates the ALK1-Smad1/5/9 pathway during osteoblastic differentiation of human pre-osteoblasts., 99th General Session & Exhibition of the IADR, 2021.07.
11.	Shinichiro Yoshida, Hideki Sugii, Masataka Kadowaki, Tomohiro Itoyama, Daigaku Hasegawa, Atsushi Tomokiyo, Sayuri Hamano, Keita Ipposhi, Kozue Yamashita, and Hidefumi Maeda., 4-META/MMA-TBB resin including nano-hydroxyapatite as a direct pulp capping material., 99th General Session & Exhibition of the IADR, 2021.07.
12.	Keita Ipposhi, Atsushi Tomokiyo, Taiga Ono, Kozue Yamashita, M, Anas Alhasan, Daigaku Hasegawa, Sayuri Hamano, Shinichiro Yoshida, Hideki Sugii, Tomohiro Itoyama, Marina Ogawa, Hidefumi Maeda, Secreted frizzled-related protein 1 promotes odontoblastic differentiation and reparative dentin formation by regulating Notch signaling in dental pulp cells., Kyudai Oral Bioscience & OBT Research Center Joint International Symposium 2021, 2021.02.
13.	Mhd Safwan Albougha, Hideki Sugii, Atsushi Tomokiyo, Sayuri Hamano, Daigaku Hasegawa, Shinichiro Yoshida, Tomohiro Itoyama, Shoko Fujino, Taiga Ono, Orie Adachi, Hidefumi Maeda. , Exosomes derived from human periodontal ligament stem cells promote osteoblastic differentiation of pre-osteoblasts., 第151回日本歯科保存学会秋季学術大会, 2019.11.
14.	Shoko Fujino, Hamano Sayuri, Atsushi Tomokiyo, Daigaku Hasegawa, Shinichiro Yoshida, Hideki Sugii, Ayako Washio, Hiromi Mitarai, Naohisa Wada, Chiaki Kitamura, Hidefumi Maeda., Effects of dopamine on odontoblastic differentiation through PKA signaling. , The 97th General Session & Exhibition of the IADR., 2019.06.
15.	Aoi Nozu, Sayuri Hamano, Atsushi Tomokiyo, Daigaku Hasegawa, Shinichiro Yoshida, Hideki Sugii, Hiromi Mitarai, Keita Ipposhi, Naohisa Wada, Hidefumi Maeda., Effects of TNF-alpha on Senescent human dental pulp cells., Kyudai Oral Bioscience & OBT Research Center Joint International Symposium 2019, 2019.03.
16.	Fujino S, Hamano S, Tomokiyo A, Hasegawa D, Yoshida Y, Sugii H, Washio A, Mitarai H, Nozu A, Arima M, Wada N, KitamuraC, Maeda H, Effects of dopamine on odontoblastic differentiation. , The IFEA 11th World Endodontic Congress 2018, 2018.10.
17.	Arima M, Hasegawa D, Yoshida S, Mitarai H, Tomokiyo A, Hamano S, Sugii H, Wada N, Maeda H. , R-spondin2 Enhances Osteoblastic Differentiation of Immature Human Periodontal Ligament Cells., The 96th General Session & Exhibition of the IADR., 2018.07.
18.	Tomokiyo A, Hamano S, Hasegawa D, Sugii H, Yoshida S, Mitarai H, Sonoda M, Nozu A, Wada N, Maeda H., Discoloration of White Mineral Trioxide Aggregate Immersed in Various Solutions., The 96th General Session & Exhibition of the IADR., 2018.07.
19.	Sugii H, Grimaldi A, Li J, Parada C, Ho T-V, Feng J, Jing J, Yuan Y, Guo Y, Maeda H, Chai Y., Dlx5 Plays a Critical Role During Soft Palate Muscle Development., The 96th General Session & Exhibition of the IADR., 2018.07.
20.	Nozu A, Hamano S, Tomokiyo A, Hasegawa D, Sugii H, Yoshida S, Mitarai H, Taniguchi S, Wada N, Maeda H., Odontoblastic differentiation of senescence dental pulp cells treated by TNF-α., The 96th General Session & Exhibition of the IADR., 2018.07.
21.	Arima M, Hasegawa D, Yoshida S, Mitarai H, Tomokiyo A, Hamano S, Sugii H, Wada N, Maeda H., R-spondin2 enhances osteogenesis of immature human periodontal ligament cells through the canonical Wnt signaling pathway., Kyudai Oral Bioscience 2018, 2018.02.
22.	Hidefumi Maeda, Differentiation and Aging of Pulp Cells., The 19th Scientific Congress of Asia Pacific Endodontic Confederation and 18th IACDE & IES PG Convention, 2017, 2017.04.
23.	Hiromi Mitarai, Naohisa Wada, Daigaku Hasegawa, Shinichiro Yoshida, Mai Arima, Atsushi Tomokiyo, Sayuri Hamano, Hidefumi Maeda, Transgelin mediates TGF-beta1-induced human periodontal ligament cell proliferation., 95th General Session & Exhibition of the IADR, 2017.03.
24.	Hiromi Mitarai, Naohisa Wada, Daigaku Hasegawa, Shinichiro Yoshida, Mai Arima, Atsushi Tomokiyo, Sayuri Hamano, Suguru Serita, Hiroyuki Mizumachi, Hidefumi Maeda, Transgelin mediates the proliferation of human periodontal ligament cells induced by TGF-β1., Kyudai Oral Bioscience 2017, 2017.02.
25.	Shinichiro Yoshida, Naohisa Wada, Daigaku Hasegawa, Atsushi Tomokiyo, Sayuri Hamano, Hiromi Mitarai, Hideki Sugii, Hidefumi Maeda, Semaphorin 3A Induces Odontoblastic Phenotype in Dental Pulp Stem Cells., 94th General Session & Exhibition of the IADR., 2016.06.
26.	Daigaku Hasegawa, Naohisa Wada, Sayuri Hamano, Atsushi Tomokiyo, Shinichiro Yoshida, Hiromi Mitarai, Mai Sonoda, Hideki Sugii, Hidefumi Maeda, Identification of a Novel Periodontal Ligament Stem Cell Marker., 94th General Session & Exhibition of the IADR., 2016.06.
27.	Sayuri Hamano, Atsushi Tomokiyo, Naohisa Wada, Daigaku Hasegawa, Hideki Sugii, Shinichiro Yoshida, Suguru Serita, Hiroyuki Mizumachi, Hiromi Mitarai, Hidefumi Maeda, Directing Human iPS Cells Toward PDL Stem Cells., 94th General Session & Exhibition of the IADR., 2016.06.
28.	Hideki Sugii, Atsushi Tomokiyo, Sayuri Hamano, Daigaku Hasegawa, 吉田晋一郎, Suguru Serita, Hiroyuki Mizumachi, Hiromi Mitarai, Aoi Nozu, Marina Ogawa, Mai Sonoda, Naohisa Wada, Hidefumi Maeda, Activin A reversely works between human pre-osteoblastic cells and periodontal ligament cells on their osteoblastic differentiation. , 2nd symposium of Program for Advancing Strategic International Networks to Accelerate the Circulation of Talented Researchers, 2016.02.
29.	Hidefumi Maeda, How can we save a severely-damaged tooth?, Kyudai Oral Bioscience 2016, 2016.02.
30.	吉田晋一郎, Naohisa Wada, Daigaku Hasegawa, Atsushi Tomokiyo, Sayuri Hamano, Hiromi Mitarai, Suguru Serita, Hiroyuki Mizumachi, Hidefumi Maeda, Semaphorin 3A induces odontoblastic phenotype in dental pulp stem cells., Kyudai Oral Bioscience 2016, 2016.02.
31.	Glial cell line-derived neurotrophic factor (GDNF) from human periodontal ligament cells treated with proinflammatory cytokines promotes neurocytic differentiation of PC12 cells..
32.	The contribution of Calcium-sensing receptor on mineralization of human dental pulp cells..
33.	Fluid analysis of laser-activated irrigation in apical ramification of the simulated canal model..
34.	Transgelin is involved in the expression of alpha-SMA in periodontal ligament cells..
35.	Generation of neural crest like-cells from human periodontal ligament- and foreskin-derived induce pluripotent stem cells..
36.	Myna N. Zakaria, Toru Takeshita, Michiko Furuta, Hidefumi Maeda, Akifumi Akamine, Yoshihisa Yamashita, Oral Mycobiome in Japanese Elderly Adults., 93rd General Session & Exhibition of the IADR. , 2015.03.
37.	Daigaku Hasegwa, Naohisa Wada, Hidefumi Maeda, Shinichiro Yoshida, Hiromi Mitarai, Atsushi Tomokiyo, Monnouchi Satoshi, Sayuri Hamano, Asuka Yuda, Akifumi Akamine, The Effects of Wnt5a on Human Periodontal Ligament Cells., 93rd General Session & Exhibition of the IADR., 2015.03.
38.	Sayuri Hamano, Hidefumi Maeda, Daigaku Hasegawa, Monnouchi Satoshi, Naohisa Wada, Atsushi Tomokiyo, Asuka Yuda, Hideki Sugii, Shinichiro Yoshida, Akifumi Akamine, Effect of β2 Adrenergic Receptor on Human Periodontal Ligament Cells. , 93rd General Session & Exhibition of the IADR., 2015.03.
39.	Asuka Yuda, Hidefumi Maeda, Fujii Shinsuke, Monnouchi Satoshi, Naohisa Wada, Atsushi Tomokiyo, Sayuri Hamano, Daigaku Hasegawa, Hideki Sugii, Shinichiro Yoshida, Akifumi Akamine, Collaborative Activity of CTGF/CCN2 and TGF-β1 on Osteogenesis and Fibrogenesis., 93rd General Session & Exhibition of the IADR., 2015.03.
40.	Daigaku　Hasegawa, naohisa wada, Hidefumi Maeda, Shinichiro Yoshida, Monnouchi Satoshi, Koori Katsuaki, Sayuri Hamano, Hiromi Mitarai, Akifumi Akamine, The Effects of Wnt5a on Human Periodontal Ligament Cells. , Kyudai Oral Bioscience 2014 —8th International Symposium —, 2014.02.
41.	Hidefumi Maeda, To seek a longer conservation method of tooth, 1st International Conference on Dental & Oral Health, 2013.08, In Japan, we meet the aging of society, and along with this, people desire to retain their teeth as long as possible. Periodontitis, caries, fracture, and orthodontic issue are known to be as major causes of tooth loss. To correct these problems, clinicians and researchers have made efforts to develop novel and unique therapies. In this context, we have focused on treatment for caries and fracture. To address this issue, we have been attempting to clarify the mechanism of the regeneration of periodontal ligament (PDL) tissue and to develop functional filling materials to permit this event. For the former agenda, we have investigated to define PDL stem cells, signaling and scaffolds based on tissue engineering standpoint. Firstly we have established and characterized two undifferentiated human PDL clonal cell lines that had been immortalized by gene transfer, and then examined the effects of various cytokines and calcium on these cell lines. For the latter agenda, we have prepared calcium-modified 4-META/MMA-TBB resin, and evaluated its bioactive features, such as biocompatibility, formation of hydroxyapatite on its surface, and differentiation-inducing activity for human PDL cells. Thus to elucidate above-described three elements optimal to allow periodontal regeneration, and to develop functional filling materials with characteristics as a scaffold for that will lead us to pioneer a new dental therapy for longer conservation of tooth. This technique may be also prospective in further application for developing the implant retaining PDL tissue or a treatment of periodontitis..
42.	ZAKARIA MN, Hidefumi Maeda, naohisa wada, Akifumi Akamine, Molecular Assessment of Bacterial Community in Persistent Apical Periodontitis Granulomatous Tissue： 16S rDNA Clone Library Analysis. , The 9th World Endodontic Congress of IFEA., 2013.05.
43.	吉田晋一郎, naohisa wada, 前田英史, 長谷川大学, 佐藤浩美, Monnouchi Satoshi, Akifumi Akamine, Dental Pulp Stem Cells on Bone Tissue Express Periodontal Ligament-related gene., The 9th World Endodontic Congress of IFEA., 2013.05.
44.	門野内聡, 前田英史, 祐田明香, 赤峰昭文, Localization of βig-h3 and Collagen Type I in Dental Pulp., The 9th World Endodontic Congress of IFEA., 2013.05.
45.	前田英史, 郡勝明, 和田尚久, 友清淳, 門野内聡, 山本直秀, 赤峰昭文, Evaluation of calcium-modified 4-META/MMA-TBB resin on its bioactive feature. , The 9th World Endodontic Congress of IFEA., 2013.05.
46.	郡勝明, 前田英史, 和田尚久, 友清淳, 門野内聡, 山本直秀, 赤峰昭文, Extracellular Calcium Regulates Osteoblastic Differentiation of Undifferentiated PDL Cells., 91st General Session & Exhibition of the IADR, 2013.03.
47.	山本直秀, 前田英史, 友清淳, 和田尚久, 門野内聡, 郡勝明, 赤峰昭文, Roles of Glial Cell-Derived Neurotrophic Factor in Periodontal Ligament Cells., 91st General Session & Exhibition of the IADR, 2013.03.
48.	和田尚久, 前田英史, 長谷川大学, S. GRONTHOS, P.M. BARTOLD, D. MENICANIN, 藤井慎介, Hiroko Wada, Atsushi Tomokiyo, Monnouchi Satoshi, Akifumi Akamine, Conversion of periodontal ligament cells into multipotent stem-like cells., 91st General Session & Exhibition of the IADR., 2013.03.
49.	前田英史, 友清淳, 郡勝明, 山本直秀, 和田尚久, 門野内聡, 濱野さゆり, 祐田明香, 赤峰昭文, CaCl2-added Resin Exerts Bioactive Effects on Periodontal Ligament Cells. , 91st General Session & Exhibition of the IADR., 2013.03.
50.	和田尚久, 前田英史, 長谷川大学, Gronthos S, Bartold PM, Menicanin D, 藤井慎介, 和田裕子, 友清淳, 門野内聡, 赤峰昭文, Induction of stem/undifferentiated cells from periodontal ligament cells by Sema3A. , The Australian Health and Medical Research Congress 2012, 2012.11.
51.	The effects of Dioxins on osteoblastic differentiation of human periodontal ligament cells.
52.	The effects of Sema3A on stemness induction of human periodontal ligament cells.
53.	Exposure to transforming growth factor-beta1 after basic fibroblast growth factor promotes the fibroblastic differentiation of human periodontal ligament stem/progenitor cell lines. .
54.	Potency of Mechanical Load in Differentiation of Periodontal Ligament Stem Cells..
55.	Stretched periodontal ligament cells up-regulate Interleukin-11 to regenerate osteoblastic metabolism..
56.	The effects of passive ultrasonic irrigation on root irrigation -SEM observation-.
57.	Stretch loading induces the expression of interleukin-11 in human periodontal ligament cells..
58.	Resin-based sealers exhibit high sealing ability; thus, they are useful for endodontic treatment. Some reports concerning cytotoxicity of the eluent from these sealers, however, have been presented. The purpose of this study was to evaluate the effects of a polymethyl methacrylate resin-based sealer (Superbond Sealer™, SB) on proliferation and osteogenic differentiation of human periodontal ligament cells (HPLCs) in vitro, compared with an epoxy resin-based sealer (AH Plus™, AH). HPLCs were obtained from two patients with informed consent. SB or AH discs washed for 7 days after setting (washed) or unwashed discs just after setting for 24 hr (unwashed) were prepared. Cells cultured on these discs were evaluated by scanning electron microscopy (SEM) and WST-1 proliferation assays. The osteogenic differentiation of HPLCs cultured on these discs was examined by gene expression analysis of osteopontin (OPN) and osteocalcin (OCN) by quantitative RT-PCR. HPLCs exhibited growth on washed SB discs, whereas unwashed SB and AH discs and washed AH discs inhibited their proliferation. SEM observation revealed that HPLCs tightly attached onto the surface of washed SB discs, although few cells were observed on other discs. HPLCs significantly upregulated gene expression of OPN and OCN when cultured on washed SB discs in osteogenic differentiation medium for 2 weeks whereas other discs did not induce their differentiation. These results indicate that, although SB initially exerted cytotoxic effects on HPLCs, these effects reduced during washing for 7 days compared to AH. Washed SB might allow HPLCs to differentiate into osteogenic cells under the osteogenic environment. This study was supported by the Ministry of Education, Culture, Sports, Science and Technology in Japan..
59.	The roles of stretch loading in human periodontal ligament cells..
60.	Contribution of mechanical load to the regeneration of human periodontal ligament tissues..
61.	The effects of calcium on differentiation of human periodontal ligament stem/progenitor cell lines..
62.	The effects of basic fibroblast growth factor on the fibroblastic differentiation of human undifferentiated periodontal ligamen cell lines..
63.	The effects of glial cell-derived neurotrophic factor on chemotaxis of human periodontal ligament cells..
64.	Immunomodulatory properties of human periodontal ligament cells and dental pulp cells..
65.	Characteristics of Superbond sealer　–Its effects on proliferation and differentiation of human periodontal ligament cells- .
66.	The roles of angiotensin II in stretched periodontal ligament cells. .
67.	Fibroblastic cells from human periapical granulation tissue preferentially form calcified matrices in boiled decalcified rat bone..
68.	Angiotensin II mediates the loading signal in human periodontal ligament cells..
69.	What are the characteristics of PDL stem cells?.
70.	Angiotensin II is involved in the loading signal in stretched human PDL cells..
71.	What characteristics do PDL stem cells have?.
72.	MTA stimulates BMP2 expression in human PDL cells. .
73.	Calcium-releasing Agent Exhibits Bioactive Effects in Endodontic Therapy..
74.	A Multipotent hPDL Cell Line Promotes Neurocytic Differentiation and Migration..
75.	MTA induces mineralization of human periodontal ligament cells..

Membership in Academic Society

Japan Society for Biomedical Gerontology
Japanese Dental Education Association
Japan Association of Microscopic Dentistry
American Association of Endodontists
Japan Society for Regenerative Medicine

Awards

Effect of agonist of antagonist of beta2 adrenergic receptor on human periodontal ligament cells.
Biocompatibility of resin-based sealers－ Comparison of Superbond sealer with AH Plus －
Evaluation of the regenerative mechanism of periodontal ligament tissue
Characteristics of Superbond sealer　–Its effects on proliferation and differentiation of human periodontal ligament cells-
The effects of TGF-β1 on proliferation and differentiation of human PDL fibroblasts and a human PDL stem/ progenitor cell line.
Effects of root-end filling materials on the osteoblast-like differentiation of human periodontal ligament cells
Influence of EMD and bFGF on mineralization in human periodontal ligament cell lines.

Educational

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