https://kyushu-u.elsevierpure.com/en/persons/mako-nakamura
　Reseacher Profiling Tool　Kyushu University Pure

https://researchmap.jp/makonakamura?lang=en
My page on researchmap website .

http://www.agr.kyushu-u.ac.jp/lab/nakamura-lab/
The Nakamura lab. HP .

https://scholar.google.com/citations?user=GYDESeYAAAAJ&hl=ja
Google Scholar .

Ph.D.

No

Muscle Biology, Developmental Biology, Cell Biology

0000-0002-6129-7560

05years00months

1. Research
My research focus is to understand mechanism of muscle stem cell development and Differentiation. There are several projects on going.
A. Smooth muscle cells.
1) Smooth muscle cell differentiation and development in chick gizzard.
2) Smooth muscle cell fate mapping
B. Skeletal muscle cells.
3) Muscle stem cell marker, Pax7 function on myocyte/adipocyte cell differentiation
4) Peroxisome specific marker Nudt7 involvement on muscle cell differentiation and muscle development


2. Education
1) Problem Based Learning I, II (Graduate School of Bioresource and Bioenvironmental Sciences）
2) Advanced Animal and Marine Science (Graduate School of Bioresource and Bioenvironmental Sciences）
3) Genetics and Evolution (School of Interdisciplinary Science and Innovation）
4) Developmental Biology (School of Interdisciplinary Science and Innovation）

3. International Education
1). Management of short-term oversea programs in school of Agriculture
2). Management of short-term inbound programs in school of Agriculture and graduate school of Bioresource and Bioenvironmental Sciences.

Research Interests

• My research focus is to understand mechanism of muscle stem cell development and Differentiation. There are several projects on going.
  A. Smooth muscle cells.
  1) Smooth muscle cell differentiation and development in chick gizzard.
  2) Smooth muscle cell fate mapping
  B. Skeletal muscle cells.
  3) Muscle stem cell marker, Pax7 function on myocyte/adipocyte cell differentiation
  4) Peroxisome specific marker Nudt7 involvement on muscle cell differentiation and muscle development
  
  keyword : Animal development, skeletal muscle, visceral smooth muscle, differentiation
  2002.04～2030.06.

Current and Past Project

• We previously showed that Semaphorin 3A (Sema3A) expression was induced when quiescent muscle satellite cells were stimulated by hepatocyte growth factor and became activated satellite cells (ASCs). However, how Sema3A regulates genes in the early phase of ASCs remains unclear. In this study, we investigated whether Sema3A signaling can regulate the early phase of ASCs, an important satellite cell stage for postnatal growth, repair, and maintenance of skeletal muscle. We showed that expression of the myogenic proliferation regulatory factors Pax7 and Myf5 was decreased in myoblasts transfected with Sema3A siRNA. These cells failed to activate expression MyoD, another myogenic proliferation regulatory factor, during differentiation. Interestingly, some of the Sema3A-depleted cells did not express Pax7 and MyoD and had enlarged nuclei and very large cytoplasmic areas. We also observed that Pax7 and Myf5 expression was increased in Myc-Sema3A overexpressing myoblasts. BrdU analysis indicated that Sema3A regulated proliferation of ASCs. These findings suggest that Sema3A signaling can modulate expression of Pax7, Myf5, and MyoD. Moreover, we found that expression of emerin, an inner nuclear membrane protein, was regulated by Sema3A signaling. Emerin was identified by positional cloning as the gene responsible for the X-linked form of Emery-Dreifuss muscular dystrophy (X-EDMD). In conclusion, our results support a role for Sema3A in maintaining ASCs through regulation, via emerin, of Pax7, Myf5, and MyoD expression.
• Myogenesis is precisely proceeded by myogenic regulatory factors. Myogenic stem cells are activated, proliferated and fused into a multinuclear myofiber. Pax7, paired box 7, one of the earliest markers during myogenesis. It has been reported that Pax7 regulates the muscle marker genes, Myf5 and MyoD toward differentiation. The possible roles of Pax7 in myogenic cells have been well researched. However, it has not yet been clarified if Pax7 itself is able to induce myogenic fate in nonmyogenic lineage cells. In this study, we performed experiments using stably expressed Pax7 in 3T3-L1 preadipocytes to elucidate if Pax7 inhibits adipogenesis. We found that Pax7 represses adipogenic markers and prevents differentiation. These cells showed decreased expression of PDGFRα, PPARγ and Fabp4 and inhibited forming lipid droplets.

Academic Activities
Membership in Academic Society

• The Japan Association for Global Competency Education

Awards

• Award for reviewing grant proposal

Educational Activities
Other Educational Activities

• 2010.08.