| 1. |
Toshifumi Minamoto, Masaki Miya, Tetsuya Sado, Satoquo Seino, Hideyuki Doi, Michio Kondoh, Keigo Nakamura, Teruhiko Takahara, Satoshi Yamamoto, Hiroki Yamanaka, Hitoshi Araki, Wataru Iwasaki, Akihide Kasai, Reiji Masuda, Kimiko Uchii, An illustrated manual for environmental DNA research: Water sampling guidelines and experimental protocols., Environmental DNA, https://doi.org/10.1002/edn3.121, 2020; 00, 1-6, 2020.07, [URL], Environmental DNA (eDNA)-based assessments of macro-organisms have now become
an essential approach for biomonitoring. eDNA survey methods have a number
of advantages over conventional survey methods. However, the value of the data that
will accumulate would be greatly enhanced by standardizing the analysis methods,
which would allow us to compare data from multiple monitoring sites at different
points in time. The eDNA Society (http://ednas ociety.org/en/about), whose founding
members consist of Japanese researchers conducting eDNA studies on macroorganisms,
was established in 2018, with the aim of expanding eDNA technology and
science. Here, we introduce our key publication, “Environmental DNA Sampling and
Experiment Manual” (http://ednas ociety.org/en/manual), which was published under
the initiative of the eDNA Society. Detailed methods for the surveys and experiments
are described in the manual, including the selection of sampling sites, sampling
methods, filtration methods, DNA extraction, species-specific detection by real-time
polymerase chain reaction, and fish eDNA metabarcoding. The manual assists users
in conducting standardized surveys and quality experiments, and provides a basis
for collecting comparable data. Given that the efficacy of methods can be context
dependent and variable, and that procedures may sometimes conflict with standardization,
it is difficult to ensure that all processes are equally effective. However, even
in such cases, it is important to maintain sufficiently high data quality by setting the minimum standards to be followed. Implementation of such standardized methodologies will enable the systematic and frequent collection of flawless, comparable eDNA data from around the world; this will provide important fundamental information for biodiversity conservation, as well as the sustainable use of fisheries resources.. |
| 2. |
Satoquo SEINO, Yukiko ASHIKAGA, Takaaki UDA, Hiroki MIHARA and Seiji WATANABE and Yasuhiro OKI, Monitoring Topographic and Habitat Changes in Natural Sand Dunes after Setting Back
Seawall at Nakatsu Tidal Flat, Oita, Japan, Asian and Pacific Coasts 2011, pp.1093-1101, 2011.12. |
| 4. |
SEINO S., UDA T. and ASHIKAGA Y., COMMUNITY-BASED COASTAL PLANNING FOR CONSERVATION OF HABITAT OF ENDANGERED SPECIES AND BIODIVERSITY IN NAKATSU TIDAL FLAT, JAPAN, Asian and Pacific Coasts 2009, Proc. 5th International Conf, 2009.11. |
| 5. |
SEINO S., KOJIMA A., HINATA H, MAGOME S. and ISOBE A., Multi-Sectoral Research on East China Sea Beach Litter Based on Oceanographic Methodology and Local Knowledge, Journal of Coastal Research, Si 56, 1289 – 1292, ISSN 0749-0258, 2009.04. |
