１．集密的な条件下で培養したヒト網膜色素上皮細胞の挙動解析に基づく成熟化の促進に関する研究（2011-2017年）
２．網膜細胞移植医療に用いるヒトiPS細胞から移植細胞への分化誘導に係わる工程及び品質管理技術の開発に関する研究（2014-2015年）
３．Optical Coherence Tomography（OCT）を用いた肝凝集塊に対する薬剤毒性を定量的かつ非侵襲的に理解するための基盤技術開発に関する研究（2018-2021年）
４．毛細胆管の動態解析に基づく非侵襲的かつ定量的な毒性評価に関する研究（2018-2022年）
キーワード：集密的条件，上皮細胞，非侵襲的評価，画像解析，再生医療，創薬
2023.01.

液-液界面上で培養した上皮細胞の均一な成熟化の促進
キーワード：液体足場材料, 上皮細胞，均一な成熟化，タイトジャンクション，細胞挙動
2023.04～2025.03.

主要原著論文

1.	Rie Sonoi, Masamichi Kamihira, A novel strategy to facilitate uniform epithelial cell maturation using liquid–liquid interfaces, Scientific Reports, 2024.05, [URL], Epithelial tissue forms and maintains a critical barrier function in the body. A novel culture design aimed at promoting uniform maturation of epithelial cells using liquid materials is described. Culturing Madin–Darby canine kidney (MDCK) cells at the liquid–liquid interface yielded reduced migration and stimulated active cell growth. Similar to solid–liquid interfaces, cells cultured on a fibronectin-coated liquid–liquid interface exhibited active migration and growth, ultimately reaching a confluent state. These cells exhibited reduced stress fiber formation and adopted a cobblestone-like shape, which led to their even distribution in the culture vessel. To inhibit stress fiber formation and apoptosis, the exposure of cells on liquid–liquid interfaces to Y27632, a specific inhibitor of the Rho-associated protein kinase (ROCK), facilitated tight junction formation (frequency of ZO-2-positive cells, FZ = 0.73). In Y27632-exposed cells on the liquid–liquid interface, the value obtained by subtracting the standard deviation of the ratio of nucleus densities in each region that compartmentalized a culture vessel from 1, denoted as HLN, was 0.93 ± 0.01, indicated even cell distribution in the culture vessel at t = 72 h. The behavior of epithelial cells on liquid–liquid interfaces contributes to the promotion of their uniform maturation..
2.	Sonoi, R. and Hagihara Y., Quantitative understanding of HepaRG cells during drug‐induced intrahepatic cholestasis through changes in bile canaliculi dynamics, Pharmacology Research & Perspectives, 10.1002/prp2.960, 2022.05, [URL].
3.	Sonoi, R. and Hagihara Y., Tight junction stabilization prevents HepaRG cell death in drug-induced intrahepatic cholestasis, Biology Open, 10.1242/bio.058606, 2021.06, [URL].
4.	Sonoi, R., Yamakawa, T., Nakatani, N., Kokubo, M., and Hagihara, Y., Noninvasive Evaluation of HepaRG Aggregates during Drug‐Induced Intrahepatic Cholestasis Using Optical Coherence Tomography, Advanced Biology, 10.1002/adbi.202000198, 5, 2, 2021.02, [URL].
5.	Sonoi, R., and Hagihara, Y., Switching of cell fate through the regulation of cell growth during drug-induced intrahepatic cholestasis, Journal of Bioscience and Bioengineering, 10.1016/j.jbiosc.2020.08.004, 130, 6, 659-665, 2020.12, [URL].
6.	Sonoi, R., Kim, M.H., Yamada, K., and Kino-oka, M., Phenotypic heterogeneity of human retinal pigment epithelial cells in passaged cell populations, Journal of bioscience and bioengineering, 10.1016/j.jbiosc.2017.03.008, 124, 2, 227-233, 2017.08, [URL].
7.	Sonoi, R., Kim, M.H., and Kino-oka, M., Facilitation of uniform maturation of human retinal pigment epithelial cells through collective movement in culture, Journal of bioscience and bioengineering, 10.1016/j.jbiosc.2015.05.019, 121, 2, 220-226, 2016.02, [URL].
8.	Sonoi, R., Kim, M.H., and Kino-oka, M., Locational heterogeneity of maturation by changes in migratory behaviors of human retinal pigment epithelial cells in culture, Journal of bioscience and bioengineering, 10.1016/j.jbiosc.2014.05.025, 119, 1, 107-112, 2015.01, [URL].
9.	Kim, M.H., Sonoi, R., Yamada, K., Inamori, M., and Kino-oka, M., Analysis of locality of early-stage maturation in confluent state of human retinal pigment epithelial cells, Journal of bioscience and bioengineering, 10.1016/j.jbiosc.2012.02.009, 113, 6, 778-781, 2012.06, [URL].

主要学会発表等

1.	園井理惠，山川健，中谷徳幸，小久保正彦，萩原義久, 光干渉断層撮影法による薬物性胆汁鬱滞を誘導したHepaRG凝集塊に対する非侵襲的評価, 第20回日本再生医療学会, 2021.03.
2.	園井理惠，萩原義久, 薬物性胆汁鬱滞を誘導したHepaRG凝集塊の細胞増殖性と毛細胆管の再生, 第20回日本再生医療学会, 2021.03.
3.	園井理惠，福永素子，鍛谷香織，百田禎郎，田中芳浩，中西洋一，岡﨑利彦, 細胞調製施設内における昆虫モニタリングにより採取同定された昆虫相から見る衛生マネジメント, 第17回日本再生医療学会, 2018.03.
4.	園井理惠，福永素子，鍛谷香織，百田禎郎，田中芳浩，中西洋一，岡﨑利彦, 細胞加工施設の管理者向け法規（製造・品質保証関連）の理解度評価クラウド型システムの開発, 第17回日本再生医療学会, 2018.03.
5.	園井理惠，福永素子，鍛谷香織，百田禎郎，田中芳浩，中西洋一，岡﨑利彦, 細胞加工施設における紙ベース原本のデータインテグリティ体制の整備, 第17回日本再生医療学会, 2018.03.
6.	園井理惠，金美海，紀ノ岡正博, 細胞遊走測定によるヒト網膜色素上皮細胞の成熟過程の解析, 化学工学会第78回春季大会, 2013.03.
7.	Sonoi, R., Kim, M.H., and Kino-oka, M. , Analysis of maturation of human retinal pigment epithelial cells in confluent state through a measurement of cell migration, Aachen-Osaka Symposium, 2012.12.
8.	園井理惠，備瀬竜馬，金美海，紀ノ岡正博, コンフルエント状態における網膜色素上皮細胞の遊走性による細胞成熟度の解析, 化学工学会第44回秋季大会, 2012.09.

所属学会名

化学工学会第44回秋季大会，バイオ部会優秀ポスター賞, 化学工学会バイオ部会, 2012.09.