Kyushu University Academic Staff Educational and Research Activities Database
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Yasuyuki Ohkawa Last modified date:2017.08.08





E-Mail
Phone
092-642-4534
Fax
092-642-6099
Academic Degree
Ph.D.
Field of Specialization
chromatin, cell differentiation
Research
Research Interests
  • Analysis of selective gene expression in organogenesis
    keyword : epigenetics chromatin deep sequencer epigenome transcriptome
    2011.08~2021.03.
  • Epigenetics Analysis in Stem Cell biology
    keyword : epigenetics chromatin deep sequencer epigenome transcriptome
    2011.08~2016.03.
Current and Past Project
  • The analysis of Genomic re-organization in myogenesis
Academic Activities
Books
1. OhkawaY., Mallappa C., Dacwag CS., Imbalzano AN. , An improved restriction enzyme accessibility assay (REAA) for analyzing changes in chromatin structure in samples of limited cell number.
, 2011.10.
2. Ohkawa Y., Mallappa C., Dacwag CS., Imbalzano AN. , Isolation of nuclei from skeletal muscle satellite cells and myofibers for use in chromatin immunoprecipitation assays.
, 2011.10.
Reports
1. de la Serna IL, Ohkawa Y, Imbalzano AN., Chromatin remodelling in mammalian differentiation: lessons from ATP-dependent remodellers., Nat Rev Genet., 2006.06.
Papers
1. Semba Y, Harada A, Maehara K, Oki S, Meno C, Ueda J, Yamagata K, Suzuki A, Onimaru, M, Nogami J, Okada S, Akashi K, Ohkawa Y, Chd2 regulates chromatin for proper gene expression toward differentiation in mouse embryonic stem cells., Nucleic Acid Researc, 2017.05.
2. Taguchi, Hiroyuki, Xie, Yan, Horikoshi, Naoki, Maehara, Kazumitsu, Harada, Akihito, Nogami, Jumpei, Sato, Koichi, Arimura, Yasuhiro, Osakabe, Akihisa, Kujirai, Tomoya, Iwasaki, Takeshi, Semba, Yuichiro, Tachibana, Taro, Kimura, Hiroshi, Ohkawa, Yasuyuki, Kurumizaka, Hitoshi, Crystal Structure and Characterization of Novel Human Histone H3 Variants, H3.6, H3.7, and H3.8, BIOCHEMISTRY, 56, 16, 2184-2196, 2017.04.
3. Kato, Daiki, Osakabe, Akihisa, Arimura, Yasuhiro, Mizukami, Yuka, Horikoshi, Naoki, Saikusa, Kazumi, Akashi, Satoko, Nishimura, Yoshifumi, Nogami, Jumpei, Maehara, Kazumitsu, Ohkawa, Yasuyuki, Matsumoto, Atsushi, Kono, Hidetoshi, Inoue, Rintaro, Sugiyama, Masaaki, Kurumizaka, Hitoshi, Crystal structure of the overlapping dinucleosome composed of hexasome and octasome, SCIENCE, 356, 6334, 205-208, 2017.04.
4. Ueda, Jun, Harada, Akihito, Urahama, Takashi, Machida, Shinichi, Maehara, Kazumitsu, Hada, Masashi, Makino, Yoshinori, Nogami, Jumpei, Horikoshi, Naoki, Osakabe, Akihisa, Taguchi, Hiroyuki, Tanaka, Hiroki, Tachiwana, Hiroaki, Yao, Tatsuma, Yamada, Minami, Iwamoto, Takashi, Isotani, Ayako, Ikawa, Masahito, et al., Testis-Specific Histone Variant H3t Gene Is Essential for Entry into Spermatogenesis, CELL REPORTS, 18, 3, 593-600, 2017.01.
5. Kuniyoshi Y, Maehara K, Iwasaki T, Hayashi M, Semba Y, Fujita M, Sato Y, Kimura H, Harada A, Ohkawa, Y, Identification of Immunoglobulin Gene Sequences from a Small Read Number of mRNA-Seq Using Hybridomas., PLoS One., 11, 10, e0165473, 2016.10.
6. Maehara K, Harada A, Sato Y, Matsumoto M, Nakayama K, Kimura H, Yasuyuki Ohkawa, Tissue-specific expression of histone H3 variants diversified after species separation, Epigenetics&Chromatin, 8, 2015.09.
7. Sugimoto N, Maehara K, Yoshida K, Yasukouchi S, Osano S, Watanabe S, Aizawa M, Yugawa T,, Kiyono T, Kurumizaka H, Ohkawa, Y, Fujita M, Cdt1-binding protein GRWD1 is a novel histone-binding protein that facilitates MCM loading through its influence on chromatin architecture., Nucleic Acids Res. , 2015.05.
8. Tomita S, Abdalla MO, Fujiwara S, Matsumori H, Maehara K, Ohkawa Y, Iwase H, Saitoh N, Nakao M, A cluster of noncoding RNAs activates the ESR1 locus during breast cancer adaptation., Nat Commun. , 6, 2015.04.
9. Harada A, Mallappa C, Okada S, Butler JT, Baker SP, Lawrence JB, Ohkawa Y, Imbalzano AN, Spatial re-organization of myogenic regulatory sequences temporally controls gene expression., Nucleic Acids Res. , 43, 4, 2015.02.
10. Harada A, Maehara K, Sato Y, Konno D, Tachibana T, Kimura H, Ohkawa Y, Incorporation of histone H3.1 suppresses the lineage potential of skeletal muscle., Nucleic Acids Res. , 43, 2, 2015.01.
11. Stasevich TJ, Hayashi-Takanaka Y, Sato Y, Maehara K, Ohkawa, Y, Sakata-Sogawa K, Tokunaga M, Nagase T, Nozaki N, James G. McNally JG, Kimura H, Regulation of RNA polymerase II activation by histone acetylation in single living cells., Nature, 516, 7530, 2014.09.
12. Harada A, Okazaki E, Okada S, Tachibana T, Ohkawa Y, Production of a monoclonal antibody for C/EBPβ: the subnuclear localization of C/EBPβ in mouse L929 cells., Monoclon Antib Immunodiagn Immunother., 33, 1, 2014.02.
13. Harada A, Hayashi M, Kuniyoshi Y, Semba Y, Sugahara S, Tachibana T, Ohkawa Y, Fujita M, Generation of a monoclonal antibody for INI1/hSNF5/BAF47. , Monoclon Antib Immunodiagn Immunother., 33, 1, 2014.02.
14. Katahira,J, Okuzaki,D, Inoue, H, Yoneda, Y, Maehara K, Ohkawa, Y, Human TREX component Thoc5 affects alternative polyadenylation site choice by recruiting mammalian cleavage factor I., Nucleic Acids Research, 41, 14, 2013.08.
15. Maehara, K, Odawara, J, Harada, A,, Yoshimi, T, Nagao, K, Obuse, C, Akashi, K, Tachibana, T, Sakata, T, Ohkawa, Y, A co-localization model of paired ChIP-seq data using a large ENCODE data set enables comparison of multiple samples., Nucleic Acids Research, 41, 1, 2013.01.
16. Kumamaru, H, Ohkawa, Y, Saiwai, H, Yamada, H, Kubota, K, Kobayakawa, K, Akashi, K, Okano, H, Iwamoto, Y, Okada,S, Direct isolation and RNA-seq reveal environment-dependent properties of engrafted neural stem/progenitor cells., Nature Communications , 3, 1140, 2012.08.
17. Harada A, Okada S, Konno D, Odawara J, Yoshimi T, Yoshimura S, Kumamaru H, Saiwai H, Tsubota T, Kurumizaka H, Akashi, K, Tachibana T, Imbalzano AN, Ohkawa, Y, Chd2 interacts with H3.3 to determine myogenic cell fate., EMBO J., 31, 13, 2012.05.
18. Odawara J, Harada A, Yoshimi T, Maehara K, Tachibana T, Seiji Okada, Akashi, K, Ohkawa, Y, The classification of mRNA expression levels by the phosphorylation state of RNAPII CTD based on a combined genome-wide approach., BMC Genomics, 12, web, 2011.10.
19. Tanaka M, Shiota M, Seiji Okada, Harada A, Odawara J, Mun S, Iwao H, Ohkawa, Y, Generation of a rat monoclonal antibody specific for hsp72., Hybridoma., 30, 4, 2011.08.
20. Ohkawa, Y, C Mallappa, CS Dacwag, AN Imbalzano, Isolation of nuclei from skeletal muscle satellite cells and myofibers for use in chromatin immunoprecipitation assays., Methods in Mol. Biol., 29, 3, 2011.06.
21. Seiji Okada, Saiwai H,, Kumamaru H, Kubota K, Harada A, Yamaguchi M, Iwamoto Y, Ohkawa, Y, Flow cytometric sorting of neuronal and glial nuclei from central nervous system tissue., Journal of Cellular Physiology., 226, 2, 2011.02.
22. Ohkawa, Y, Yoshimura S, Higashi C, Marfella CG, Dacwag CS, Tachibana T, Imbalzano AN, Myogenin and the SWI/SNF ATPase Brg1 maintain myogenic gene expression at different stages of skeletal myogenesis., J Biol Chem., 282, 9, 2007.03.
23. de la Serna IL, Ohkawa, Y, Imbalzano AN, Chromatin remodelling in mammalian differentiation: lessons from ATP-dependent remodellers., Nat Rev Genet., 7, 6, 2006.06.
24. Ohkawa, Y, Marfella CG, Imbalzano AN, Skeletal muscle specification by myogenin and Mef2D via the SWI/SNF ATPase Brg1., EMBO J., 25, 3, 2006.02.
25. Ohkawa, Y, de la Serna IL, Berkes CA, Bergstrom DA, Dacwag CS, Tapscott SJ, Imbalzano AN, MyoD targets chromatin remodeling complexes to the myogenin locus prior to forming a stable DNA-bound complex., Mol Cell Biol, 25, 10, 2005.05.
Works, Software and Database
1. .
Presentations
1. 大川 恭行, Myogenic Chromatin Structure Is Formed with the Histone H3 Variant H3mm7, Gordon Research Conferenc, 2017.06.
2. 大川 恭行, ChiLT-an Immunoprecipitation-free Epigenome Profiling Technology, Single Cell Omics (E3), 2017.05.28.
3. 大川 恭行, The baselines of transcription levels are determined by selective incorporation of histone H3 variants., Transcriptional and Epigenetic Control in Stem Cells (J1), 2017.01.08.
4. 大川 恭行, N6-methyladenosine is required for the processing of MyoD pre-mRNA for maintaining skeletal muscle differentiation potential., Keystone Symposia Chromatin and Epigenetics (C2), 2016.03.
5. 大川 恭行, Cell fate decision on chromatin by MyoD, BMB2010(第38回日本分子生物学会年会・第88回日本生化学会大会合同大会), 2015.12.02.
6. 大川 恭行, The Diversity of Mouse Histone H3 Variants, International Symposium on Chromatin Structure, Dynamics, and Function, 2015.08.23.
7. 原田 哲仁, 前原 一満, 佐藤 優子, 木村 宏, 大川 恭行, Incorporation of Histone H3 Variants Dictates the Lineage Potential of Skeletal Muscle, FASEB;Science Research Conferences, 2014.07.22.
8. 前原 一満, 大川 恭行, Exploring nucleosome positioning patterns act as a functional component of chromatin structure, ENBO Workshop Histone varisnts, 2014.06.03.
9. 原田 哲仁, 前原 一満, 大川 恭行, Incorporation of Histone H3 Variants Dictates Myogenesis, ENBO Workshop Histone varisnts, 2014.06.03.
10. 大川 恭行, Diversity of histone H3 variants on mouse genome, ENBO Workshop Histone varisnts, 2014.06.03.
11. 原田 哲仁, 前原 一満, 大川 恭行, The balance of histone H3 variants around transcription start sites dictates cell differential potential, Keystone Symposia , 2014.02.06.
12. 前原 一満, 大川 恭行, The formation of nucleosome positioning patterns flanked by transcription factor binding site, Keystone Symposia , 2014.02.06.
13. 大川 恭行, Epigenomic approach unveils cell fate decision, 日本遺伝学会第85回大会, 2013.09.21.
14. 大川 恭行, High order chromatin regulation in skeletal muscle differentiation, 第86回日本生化学会大会, 2013.09.12.
15. 大川 恭行, Histone bariants determine the lineage potential of skeletal muscle, Fukuoka Internasional Symposium on Genomics & Epigenomics 2013, 2013.09.10.
16. Genomic clustering accompanies cellular differentiation to temporally control gene expression.
Membership in Academic Society
  • about The Japanese Biochemical Sicuety
  • Japan Society for Biology
  • The Molecular Biology Society of Japan