Kyushu University Academic Staff Educational and Research Activities Database
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Keitaro Suyama Last modified date:2018.07.24

Assistant Professor / Laboratory of Molecular Biochamistry
Division for Experimental Natural Science
Faculty of Arts and Science




E-Mail
Phone
092-802-5849
Academic Degree
Doctor of Science
Country of degree conferring institution (Overseas)
No
Field of Specialization
biochemistry, organic chemistry
Total Priod of education and research career in the foreign country
00years00months
Research
Research Interests
  • Coacervation Property and Secondary Structure of the Elastin-derived Synthetic Dimer Peptides
    keyword : elastin peptide coacervation
    2013.03~2017.03.
  • Asymmetric reaction caltalyzed by aluminum(salalen) complex
    keyword : asymmetric catalytic reaction, aluminum(salalen) complex
    2006.04~2009.03.
  • Probe exploitation for essential nuclear receptor-binding assay
    keyword : nuclear receptor, receptor binding assay, fluorescent probe, bisphenol
    2009.04.
Academic Activities
Reports
1. In order to provide new and field crossing subjects for active learners, we developed a new experimental subject focusing on amino acids. To strengthen the learners’ thinking power in a manner that is not limited to the confines of the existing subjects, this subject was designed to contain a variety of experiments concerning chemistry, biology, nutrition, and computer science. In the second semester of 2015, we carried out the subject as a trial class. Twelve students belonging to five different undergraduate schools attended this course. To achieve the purpose of this class, we provided eight lessons incorporating lectures, basic experiments, computer simulations, and observations using analytical equipment. To evaluate this subject, we surveyed students’ attitudes toward the class. In the questionnaire responses, we found that many students felt that the number of lectures containing experimental activities should be increased in general subjects to help them grow as active learners..
Papers
1. Keitaro Suyama, Daiki Tatsubo, Hitoshi Kesamaru, Iori Maeda, Takeru Nose, Coacervation Property and Structural Analysis of Cyclic Analogs of Elastin-derived Peptide (FPGVG)n, Peptide Science 2016, 101-102, 2017.03, The elastin-derived peptide analog, (FPGVG)5, demonstrates a temperature- dependent self-association (coacervation) property. In this study, we prepared cyclic (FPGVG)n (n=1-5) analogs and investigated their coacervation and structural properties to assess their potential for use as a biomaterial. We found that the cyclic (FPGVG)n (n=3-5) analogs exhibited high coacervation ability. The results from the molecular dynamics simulation suggest that turn structures were important for coacervation of elastin-derived cyclic peptide analogs..
2. Keitaro Suyama, Suguru Taniguchi, Daiki Tatsubo, Iori Maeda, Takeru Nose, Dimerization effects on coacervation property of an elastin-derived synthetic peptide (FPGVG)5, Journal of Peptide Science, 10.1002/psc.2876, in press, 2016.03, A series of elastin-derived peptide (Phe-Pro-Gly-Val-Gly)5 dimers possessing high coacervation potential were synthesized. The new dimeric peptides showed significantly high coacervation ability compared to known elastin-derived peptide analogs. The molecular dynamics calculation results reveal that the dimeric peptides contain characteristic sheet-turnsheet motif involving a type II β-turn-like structure and form globular conformation..
3. Daiki Tatsubo, Keitaro Suyama, Takeru Nose, Fluorescence Analysis Using a Molecular Probe 1,8-ANS for Elucidation of the Molecular Mechanisms Underlying Coacervation of a Tryptophan-containing Elastin derived Dimeric Peptide, Peptide Science 2015, 95-96, 2016.03, A tryptophan-containing elastin-derived dimeric peptide (C(WPGVG)3)2 shows potent self-assembly activity. To elucidate the underlying coacervation mechanisms, we measured the fluorescence of (C(WPGVG)3)2 by using 1,8-ANS as a fluorescent probe. The results revealed that the peptide forms microaggregates at temperature and concentration lower than those at which visible coacervation occurs. These findings suggest that microaggregate formation is unrelated to the maturation of the coacervate drop, which is temperature-dependent..
4. Keitaro Suyama, Hitoshi Kesamaru, Daiki Tatsubo, Takeru Nose, Coacervation Properties and Structural Analysis of Aminobenzoyl-labeled Fluorescent Elastin-derived Peptides, Peptide Science 2015, 293-294, 2016.03, The elastin-derived peptide analog, (FPGVG)5, demonstrates a temperature- dependent self-association (coacervation) property. In this study, we prepared fluorescent-labeled (FPGVG)5 analogs, and evaluated their coacervation and fluorescent properties in order to assess their potential for use as a base material for fluorescent thermometers. We found that the fluorescent-labeled (FPGVG)5 analogs exhibited high coacervation ability. Results from the molecular dynamics calculation suggest that the fluorescent group is exposed on the surface of the molecule..
5. Yumi Kramitsu, Hirokazu Nishimura, Ryo Nakamura, Keitaro Suyama, Kazuhiro Matsumoto, Takeru Nose, Yasuyuki Shimohigashi, High-precision binding assay procedure of tachykinin receptor NK-1 for highly potent substance P analogs, Peptide Science 2012, in press, 2013.03.
6. Kanako Nishio, Hirokazu Nishimura, Keitaro Suyama, Takeru Nose, Yasuyuki Shimohigashi, Halogenated Phe-containing endomorphin-2 analogs with mixed agonist and antagonist activities, Peptide Science 2012, in press, 2013.03.
7. Kanako Nishio, Hirokazu Nishimura, Keitaro Suyama, Yoshinori Abe, Takeru Nose, Ayami Matsushima, Yasuyuki Shimohigashi, Effects of the Halogenation of Phe-Phenyl Group of Two Consecutive Residues in Endomorphin-2 on the Interaction with the μ-Opioid Receptors, Peptide Science 2011, 171-172, 2012.03.
8. Ryo Nakamura, Hirokazu Nishimura, Keitaro Suyama, Takeru Nose, Yasuyuki Shimohigashi, The Effect of Halogenation of Phe-Phenyl Group of Two Consecutive Phe Residues Present in Neuropeptide Substance P on Its Specific Receptor Interaction, Peptide Science 2011, 157-158, 2012.03.
9. Keitaro Suyama, Yoshifumi Sakai, Kazuhiro Matsumoto, Tsutomu Katsuki, Highly Enantioselective Hydrophosphonylation of Aldehydes: Base-Enhanced Aluminum-salalen Catalysis, Angewante Chemie, International Edition, 10.1002/anie.200905158, 49, 797-799, 2010.01.
10. Keitaro Suyama, Kazuhiro Matsumoto, Tsutomu Katsuki, Asymmetric Lewis Acid Catalysis of Aluminum(salalen) Complexes: Friedel-Crafts Reaction of Indole, Heterocycles, 10.3987/COM-08-S(F)93, 77, 817-824, 2009.02.
Presentations
1. Self-assembly properties and structural analyses of fluorescent-labeled analogs of elastin-derived synthetic peptide (FPGVG)5.
2. Coacervation and fluorescence properties of fluorescent-labeled elastin-derived peptides.
3. 巣山 慶太郎, 田坪大来, 袈裟丸 仁志, 野瀬 健, Coacervation Properties and Structural Analysis of Aminobenzoyl-labeled Fluorescent Elastin-derived Peptides, 第52回ペプチド討論会, 2015.11, Biological reactions within living cells are affected by temperature. Therefore, mapping the intracellular temperature of living cells is expected to contribute to an improved understanding of cellular events and the establishment of novel therapeutics. Previously, Uchiyama et al. demonstrated intracellular temperature mapping based on a fluorescent polymeric thermometer consisting mainly of acrylamide derivatives. Elastin-derived peptides exhibit reversible self-association (coacervation) under physiological conditions. Therefore, it was considered that these peptides might serve as useful materials for the development of such a fluorescent thermometer. Recently, we reported that the short synthetic elastin-derived peptide (FPGVG)5 demonstrates coacervation, which suggests that fluorescent-labeled (FPGVG)5 peptides, in particular, may be useful as basic materials for developing a novel fluorescent peptide-based thermometer. In the present study, we synthesized fluorescent-labeled analogs of (FPGVG)5 and investigated their coacervation property and peptide conformation..
4. 巣山 慶太郎, 野瀬 健, 谷口 卓, 前田衣織, 河野敬一, エラスチン由来ペプチドダイマーの自己集合特性および立体構造の解析, 分子・物質合成PF H25年度シンポジウム , 2014.03.
5. 巣山 慶太郎, 谷口 卓, 前田衣織, 田坪大来, 野瀬 健, Coacervation Property and Secondary Structure of Synthetic Dimer Peptides of the Elastin-derived Pentapeptide Repeat-related Peptide, 4th Asia-Pacific International Peptide symposium 2013/50th Japanese Peptide Symposium , 2013.11, Elastin is the core protein of elastic fibers in the elastic tissues plays an essential role in tissue biomechanics, providing the extensibility and condensability. Elastin shows coacervation (reversible association/dissociation phenomena) under physiological conditions. Tropoelastin, the precursor protein of elastin, contains characteristic repetitive sequences such as the pentapeptide sequence, Val-Pro-Gly-Val-Gly (VPGVG), in its hydrophobic regions. However, it was reported that large molecular size was needed for coacervation of elastin-derived peptide (VPGVG)n (numbers of repetition: n>40). Recently, we reported that synthetic hydrophobic oligomers (IPGVG)n and (FPGVG)n demonstrate coacervation at significantly smaller repetition numbers (n=5~7). Because of their low molecular size, these analogs are expected to be useful as model peptides for structure analysis and base materials for developing various biomedical products. In this study, to obtain more short coacervatable peptides, we synthesized dimers of pentapeptide (FPGVG)5 and investigated their coacervation property and secondary structures..
6. Keitaro Suyama, Kazuhiro Matsumoto, Tsutomu Katsuki, Asymmetric Lewis Acid Catalysis of Aluminum(salalen) Complexes: Friedel-Crafts Reaction of Indole, 19th Joint Seminar of the Busan Branch of the Korean Chemical Society and the Kyushu Branch of the Chemical Society of Japan, 2009.05.
Educational
Other Educational Activities
  • 2013.04.
  • 2013.06.