Updated on 2025/04/17

Information

 

写真a

 
LIU CHENCHEN
 
Organization
Faculty of Science Department of Chemistry Assistant Professor
Title
Assistant Professor
Contact information
メールアドレス
Homepage
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Degree

  • University of Shanghai for Science and Technology, Doctoral Degree

  • Kyoto University, Doctoral Degree

Research History

  • 2021年10月 - 2022年03月　京都大学 大学院工学研究科 材料化学専攻 ポスドク 2016年10月 - 2018年10月　華東理工大学 ポスドク   

    2021年10月 - 2022年03月　京都大学 大学院工学研究科 材料化学専攻 ポスドク 2016年10月 - 2018年10月　華東理工大学 ポスドク

Awards

  • 第74回日本電気泳動学会学術大会　一般演題優秀賞

    2023.5   第74回日本電気泳動学会学術大会   The 74th Annual Meeting of the Japanese Electrophoresis Society, General Presentation Excellence Award

Papers

  • Recent advances in microscale separation techniques for glycome analysis Invited Reviewed

    Chenchen Liu, Koji Otsuka, Takayuki Kawai

    Journal of Separation Science   47 ( 11 )   e2400170   2024.6   （ ISSN:1615-9306 eISSN:1615-9314 ）

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：Wiley  

    The glycomic analysis holds significant appeal due to the diverse roles that glycans and glycoconjugates play, acting as modulators and mediators in cellular interactions, cell/organism structure, drugs, energy sources, glyconanomaterials, and more. The glycomic analysis relies on liquid‐phase separation technologies for molecular purification, separation, and identification. As a miniaturized form of liquid‐phase separation technology, microscale separation technologies offer various advantages such as environmental friendliness, high resolution, sensitivity, fast speed, and integration capabilities. For glycan analysis, microscale separation technologies are continuously evolving to address the increasing challenges in their unique manners. This review discusses the fundamentals and applications of microscale separation technologies for glycomic analysis. It covers liquid‐phase separation technologies operating at scales generally less than 100 µm, including capillary electrophoresis, nanoflow liquid chromatography, and microchip electrophoresis. We will provide a brief overview of glycomic analysis and describe new strategies in microscale separation and their applications in glycan analysis from 2014 to 2023.

    DOI： 10.1002/jssc.202400170

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  • Development of transient trapping micellar electrokinetic chromatography coupled with mass spectrometry for steroids analysis Invited Reviewed International journal

    Yanawut Manmana, Chenchen Liu, Hiroshi Koino, Kenji Sueyoshi, Fumihiko Kitagawa, Takuya Kubo*, Koji Otsuka

    Chirality   34 ( 10 )   1328 - 1337   2022.6

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1002/chir.23489

  • Specific recognition of a target protein, cytochrome c, using molecularly imprinted hydrogels Reviewed International journal

    Chenchen Liu, Takuya Kubo, Koji Otsuka

    Journal of Materials Chemistry B   2022.6

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1039/d2tb00501h

  • Fluorescent detection of target proteins via a molecularly imprinted hydrogel

    Takuya Kubo, Naoki Watanabe, Seiji Ikari, Chenchen Liu, Eisuke Kanao, Toyohiro Naito, Tomoharu Sano, Koji Otsuka

    ANALYTICAL METHODS   13 ( 27 )   3086 - 3091   2021.7

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    Language：English   Publishing type：Research paper (scientific journal)  

    Proteins are typically separated by an immune reaction, such as an enzyme-linked immunosorbent assay, and are detected by selective fluorescent labeling. This has potential for complicated procedures and the denaturation of proteins by labeling, and is cost consuming. In this study, we propose a technique for the selective separation and detection of a target protein using a molecularly imprinted hydrogel (PI gel) with fluorescent monomers. We focused on 8-anilino-1-naphthalenesulfonic acid (ANS), where the fluorescence intensity is easily changed by the interaction with proteins, and successfully synthesized the ANS monomer and a poly(ethylene glycol) (PEG) conjugated ANS monomer. The PI gel with the ANS monomers using bovine serum albumin (BSA) as a template showed the selective adsorption of BSA and the fluorescence intensity increased due to the adsorption of BSA.

    DOI： 10.1039/d0ay02341h

  • Hydrogels in Electrophoresis: Applications and Advances

    Chenchen Liu, Takuya Kubo, Koji Otsuka

    ANALYTICAL SCIENCES   37 ( 6 )   807 - 816   2021.6

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    Language：English   Publishing type：Research paper (scientific journal)  

    A hydrogel is a solid form of polymer network absorbed in a substantial amount of aqueous solution. In electrophoresis, hydrogels play versatile roles including as support media, sieving matrixes, affinity scaffolds, and compositions of molecularly imprinting polymers. Recently, the study of hydrogels has been advancing with unprecedented speed, and the application of hydrogels in separation science has brought new opportunities and possible breakthroughs. A good understanding about the roles and effects of the material is essential for hydrogel applications. This review summarizes the hydrogels that has been described in various modes of electrophoretic separations, including isoelectric focusing gel electrophoresis (IEFGE), isotachophoresis (ITP), gel electrophoresis and affinity gel electrophoresis (AGE). As microchip electrophoresis (ME) is one of the future trends in electrophoresis, thought provoking studies related to hydrogels in ME are also introduced. Novel hydrogels and methods that improve separation performance, facilitate the experimental operation process, allow for rapid analysis, and promote the integration to microfluidic devices are highlighted.

    DOI： 10.2116/analsci.20R004

  • Controllable Molecular Sieving by copoly(Poly(ethylene glycol) Acrylate/Poly(ethylene glycol) Diacrylate)-Based Hydrogels via Capillary Electrophoresis for DNA Fragments

    Chenchen Liu, Takuya Kubo, Toyohiro Naito, Koji Otsuka

    ACS Applied Polymer Materials   2 ( 9 )   3886 - 3893   2020.9

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    Language：Others   Publishing type：Research paper (scientific journal)  

    DOI： 10.1021/acsapm.0c00567

  • Online fluorescence imaging method by reducing the inequivalent photobleaching for quantitative capillary electrophoresis

    Chenchen Liu, Yoshinori Yamaguchi, Yi Wang, Xiaoming Dou, Takuya Kubo, Koji Otsuka

    SENSORS AND ACTUATORS B-CHEMICAL   319   2020.9

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.snb.2020.128035

  • Rapid and quantitative detection of trace Sudan black B in dyed black rice by surface-enhanced Raman spectroscopy (SERS)

    Yubin Zhao, Yoshinori Yamaguchi, Chenchen Liu, Mingda Li, Xiaoming Dou

    SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY   216   202 - 206   2019.6

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    Language：English   Publishing type：Research paper (scientific journal)  

    The use of Sudan black B as coloring agent in foods is forbidden for its toxicology effect on human organs. This work proposes an efficient and sensitive method for food security inspection targeting Sudan black B. Surface-enhanced Raman spectroscopy (SERS) is applied to the analysis of trace Sudan black B. It could be detected at concentrations as low as 0.05 mg/L in standard solutions and 0.1 mg/kg in black rice extracts with the SERS method for measurement. The linear relationship between the intensity and concentration could be used for the quantitative detection of Sudan black B. The relation between dyeing time of black rice stained by Sudan black B solution and SERS intensity was studied which indirectly showed the effectiveness of the extraction method we designed. The results of the quantitative analysis reveal the practicability of using this method to detect Sudan black B in black rice. As a rapid and sensitive detection method, SERS can be extended to detect other food products and has a great application prospect in food safety inspection. (C) 2019 Elsevier B.V. All rights reserved.

    DOI： 10.1016/j.saa.2019.03.030

  • Precise and simultaneous enumeration of multiplex pathogens using multiplex polymer chain reaction coupled with a novel quantitative capillary electrophoresis

    Liu, C., Yamaguchi, Y., Sekine, S., Ni, Y., Dou, X.

    Sensors and Actuators, B: Chemical   258   2018.4

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    Language：Others   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.snb.2017.11.106

  • Quantitative Detection of Ethanol/Acetone in Complex Solutions Using Raman Spectroscopy Based on Headspace Gas Analysis

    Yubin Zhao, Yoshinori Yamaguchi, Chenchen Liu, Shinichi Sekine, Xiaoming Dou

    APPLIED SPECTROSCOPY   72 ( 2 )   280 - 287   2018.2

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    Language：English   Publishing type：Research paper (scientific journal)  

    This paper demonstrated the quantitative detection of ethanol and acetone mixtures in complex solutions with Raman spectroscopy based on headspace gas analysis. By analyzing the volatile components in the headspace, their concentrations in liquid solutions were determined. We constructed our own Raman spectroscopy system to detect the headspace gas quantitatively over a solution in a sealed vial. The Raman spectra of the headspace gases over standard solutions were standardized for finding the concentrations of ethanol, acetone, and ethanol-acetone in mixture solutions. The results showed that the concentration of a gaseous component in the headspace gas was proportional to its ratio in the liquid solution. We obtained a linear relationship between the spectral intensity of volatile components in headspace and the concentration of the liquid solutions. Then, we analyzed the alcohol concentration in a white wine and a Chinese liquor called Fen Chiew by measuring the Raman spectra of the headspace gas over their liquids. For the river water sample, we also implemented our headspace gas detection with Raman spectra to obtain the concentration of acetone in the river sample. This work demonstrated the facilitation of headspace gas analysis by the qualitative and quantitative determination of volatile substances from liquid samples using Raman spectroscopy.

    DOI： 10.1177/0003702817738010

  • Analysis of Capillary Electrophoresis Noise Characteristics

    Chen, G., Zhu, X.-F., Dou, X.-M., Liu, C.-C., Xu, Q.-Q., Yamaguchi, Y., Ni, Y.

    Guangzi Xuebao/Acta Photonica Sinica   46 ( 6 )   2017.6

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    Language：Others   Publishing type：Research paper (scientific journal)  

    DOI： 10.3788/gzxb20174606.0612004

  • Molecular sieving polymer for DNA/RNA separation in capillary electrophoresis

    Liu, C., Yamaguchi, Y., Dou, X.

    International Journal of Modern Physics B   31 ( 16-19 )   2017.5

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    Language：Others   Publishing type：Research paper (scientific journal)  

    DOI： 10.1142/S0217979217440945

  • Effective length calibration method for processing the fluorescence signal detected by charge-coupled device in capillary electrophoresis

    Ni, Y., Liu, C., Chen, J., Chen, Q., Zhu, X., Dou, X.

    Journal of Separation Science   40 ( 9 )   2017.3

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    Language：Others   Publishing type：Research paper (scientific journal)  

    DOI： 10.1002/jssc.201601458

  • Rapid identification and quantitation for oral bacteria based on short-end capillary electrophoresis

    Chen, J., Ni, Y., Liu, C., Yamaguchi, Y., Chen, Q., Sekine, S., Zhu, X., Dou, X.

    Talanta   160   2016.11

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    Language：Others   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.talanta.2016.07.049

  • Analysis of the inhibition of nucleic acid dyes on polymerase chain reaction by capillary electrophoresis

    Li, Z., Liu, C., Ma, S., Zhang, D., Yamaguchi, Y.

    Analytical Methods   8 ( 11 )   2016.2

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    Language：Others   Publishing type：Research paper (scientific journal)  

    DOI： 10.1039/c5ay02705e

  • Capillary electrophoresis of RNA in hydroxyethylcellulose polymer with various molecular weights

    Li, Z., Liu, C., Zhang, D., Luo, S., Yamaguchi, Y.

    Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences   1011   2016.1

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    Language：Others   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.jchromb.2015.12.057

  • Gene analysis of multiple oral bacteria by the polymerase chain reaction coupled with capillary polymer electrophoresis

    Liu, C., Yamaguchi, Y., Sekine, S., Ni, Y., Li, Z., Zhu, X., Dou, X.

    Journal of Separation Science   39 ( 5 )   2015.12

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    Language：Others   Publishing type：Research paper (scientific journal)  

    DOI： 10.1002/jssc.201501087

  • Theoretical investigation on the performance of DNA electrophoresis under programmed step electric field strength: Two-step condition

    Ni, Y., Liu, C., Chen, Q., Zhu, X., Dou, X.

    Journal of Separation Science   38 ( 20 )   2015.10

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    Language：Others   Publishing type：Research paper (scientific journal)  

    DOI： 10.1002/jssc.201500640

  • Polyethylene Oxide (PEO) and Polyethylene Glycol (PEG) Polymer Sieving Matrix for RNA Capillary Electrophoresis (vol 10, e0123406, 2015)

    Yoshinori Yamaguchi, Zhenqing Li, Xifang Zhu, Chenchen Liu, Dawei Zhang, Xiaoming Dou

    PLOS ONE   10 ( 6 )   2015.6

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1371/journal.pone.0131265

  • Analysis of small interfering RNA by capillary electrophoresis in hydroxyethylcellulose solutions

    Liu, C., Yamaguchi, Y., Zhu, X., Zhenqing, L., Ni, Y., Dou, X.

    Electrophoresis   36 ( 14 )   2015.4

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    Language：Others   Publishing type：Research paper (scientific journal)  

    DOI： 10.1002/elps.201500018

  • Quantification of Periodontal Pathogens Cell Counts by Capillary Electrophoresis

    Li, Z., Chen, S., Liu, C., Zhang, D., Dou, X., Yamaguchi, Y.

    Journal of Chromatography A   1361   2014.8

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    Language：Others   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.chroma.2014.07.100

  • Electromigration behavior of nucleic acids in capillary electrophoresis under pulsed-field conditions

    Li, Z., Liu, C., Dou, X., Ni, Y., Wang, J., Yamaguchi, Y.

    Journal of Chromatography A   1331   2014.3

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    Language：Others   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.chroma.2014.01.021

  • Capillary electrophoresis of a wide range of DNA fragments in a mixed solution of hydroxyethyl cellulose

    Li, Z., Liu, C., Yamaguchi, Y., Ni, Y., You, Q., Dou, X.

    Analytical Methods   6 ( 8 )   2014.1

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    Language：Others   Publishing type：Research paper (scientific journal)  

    DOI： 10.1039/c3ay41965g

  • Capillary electrophoresis of DNA in hydroxyethylcellulose

    Liu, C., Li, Z., Meng, F., Ni, Y., Dou, X., Yoshinori, Y.

    Acta Chimica Sinica   71 ( 2 )   2012.12

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    Language：Others   Publishing type：Research paper (scientific journal)  

    DOI： 10.6023/A12110935

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Research Projects

  • Single-cell Mitochondria Analysis using Molecularly Imprinted Material

    Grant number：23K13776  2023 - 2024

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Early-Career Scientists

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    Authorship：Principal investigator  Grant type：Scientific research funding

  • Functionalized capillary device for mass spectrometry-based subcellular lipid profiling

    Grant number：22K20560  2022 - 2023

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Research Activity start-up

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    Authorship：Principal investigator  Grant type：Scientific research funding

  • 分子認識PEGゲルキャピラリー電気泳動による生体分子の構造・サイズ選択的分離

    Grant number：20J15151  2020 - 2021

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

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    Grant type：Scientific research funding

  • キャピラリー電気泳動の分離性能の向上の研究-新しいポリマーゲルを使用した一本鎖コンフォメーション多型(google translation)

    Grant number：21804042  2017 - 2021

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

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    Authorship：Principal investigator  Grant type：Scientific research funding