Updated on 2024/10/01

Information

 

写真a

 
IKENOUCHI JUNICHI
 
Organization
Faculty of Medical Sciences Department of Basic Medicine Professor
School of Sciences Department of Biology(Concurrent)
Graduate School of Systems Life Sciences Department of Systems Life Sciences(Concurrent)
Title
Professor
Profile
In textbooks, schematic diagrams of cells depict the plasma membrane as two gray lines. However, in reality, the membrane is composed of thousands of distinct lipid molecules. Why do cells produce and utilize such a vast array of lipid species? To answer this question, my research focuses on elucidating the functions of lipids, particularly in the context of cell adhesion and polarity formation in epithelial cells. Additionally, I investigate the role of lipids in pathological conditions such as cancer and fibrosis, which arise from abnormalities in epithelial cells. For undergraduate and graduate students, I provide guidance on key topics in cell biology and biochemistry through lectures, daily laboratory experiments, journal clubs, conference presentations, and research publications. I aim to teach not only the historical context and recent advancements of major discoveries but also the methodology of conducting research and summarizing findings.
Homepage
External link

Degree

  • M.D.Ph.D.

Research History

  • 無し

    無し

  • 2008/05/01〜2010/07/31 京都大学化学研究所 准教授 2010/08/01〜2013/06/30 京都大学大学院工学研究科 准教授

Research Interests・Research Keywords

  • Research theme:Elucidation of roles of lipids in the formation of epithelial polarity and cell adhesion Elucidation of the organizing principles of living cell membrane by combining synthetic biological approaches and cell biological approaches

    Keyword:Epithelial cells, Tight junction, Microvilli, Cell polarity, Biological membrane, Cytoskeleton

    Research period: 2002.4 - 2023.9

Awards

  • 第17回 風戸賞

    2024.3   風戸研究奨励会  

  • 花王科学賞

    2022.6   公益財団法人 花王芸術・科学財団   上皮細胞の細胞膜構造形成に関する研究

  • 第12回柿内三郎記念奨励研究賞

    2015.12   公益社団法人日本生化学会  

  • 第7回井上リサーチアウォード

    2014.12   井上科学振興財団   池ノ内順一氏は上皮細胞の細胞接着や細胞極性の研究領域に於いて独自性の高い貢献を果たしてきた。池ノ内氏は、上皮間葉転換現象に着目して、上皮細胞にのみ特異的に発現する遺伝子の絞り込みを行った。その中から新たな細胞接着分子 Tricelluin や細胞極性の形成に関わる新規分子 FRMD4A を同定し、それらの機能を解明した。池ノ内氏の発見した Tricellulin は3つの上皮細胞の間で形成される細胞接着構造を構成する細胞接着分子として世界で最初に同定された膜タンパク質である。その成果は、Molecular Biology of the Cell などの教科書に掲載され、世界的に高く評価されている。

  • 科学技術分野の文部科学大臣表彰 若手科学者賞

    2014.4   文部科学省  

Papers

  • A sustained calcium response mediated by IP3 receptor anchoring to the desmosome is essential for apoptotic cell elimination.

    Cho Y, Koyama-Honda I, Tanimura A, Matsuzawa K, Ikenouchi J

    Current Biology   2024.9   ISSN:0960-9822

     More details

    Language:English  

    DOI: 10.1016/j.cub.2024.08.057

    PubMed

  • Cholesterol-rich domain formation mediated by ZO proteins is essential for tight junction formation Reviewed International journal

    Shigetomi K, Ono Y, Matsuzawa K, Ikenouchi J.

    Proc Natl Acad Sci U S A   120 ( 8 )   e2217561120   2023.2

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1073/pnas.2217561120.

    Other Link: https://www.pnas.org/doi/abs/10.1073/pnas.2217561120

  • mTORC2 suppresses cell death induced by hypo-osmotic stress by promoting sphingomyelin transport Invited Reviewed International journal

    #Yumiko Ono, @Kenji Matsuzawa, @Junichi Ikenouchi

    The Journal of Cell Biology   221 ( 4 )   2022.1   ISSN:0021-9525 eISSN:1540-8140

     More details

    Language:Japanese   Publishing type:Research paper (scientific journal)  

    DOI: 10.1083/jcb.202106160

    Web of Science

    Scopus

    PubMed

  • Tricellulin secures the epithelial barrier at tricellular junctions by interacting with actomyosin Invited Reviewed International journal

    #Yuma Cho, #Daichi Haraguchi, @Kenta Shigetomi, @Kenji Matsuzawa, @Seiichi Uchida, @Junichi Ikenouchi

    The Journal of Cell Biology   221 ( 4 )   2022.1   ISSN:0021-9525 eISSN:1540-8140

     More details

    Language:Japanese   Publishing type:Research paper (scientific journal)  

    DOI: 10.1083/jcb.202009037

    Web of Science

    Scopus

    PubMed

  • MAGIs regulate aPKC to enable balanced distribution of intercellular tension for epithelial sheet homeostasis Reviewed International journal

    @Matsuzawa, Kenji; #Ohga, Hayato; @Shigetomi, Kenta; @Shiiya, Tomohiro; @Hirashima, Masanori; @Ikenouchi, Junichi

    COMMUNICATIONS BIOLOGY   4 ( 1 )   2021.3

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1038/s42003-021-01874-z

  • STIM-Orai1 signaling regulates fluidity of cytoplasm during membrane blebbing Invited Reviewed International journal

    Aoki, Kana; #Harada, Shota; #Kawaji, Keita; Matsuzawa, Kenji; Uchida, Seiichi; Ikenouchi, Junichi

    NATURE COMMUNICATIONS   12 ( 1 )   2021.1

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1038/s41467-020-20826-5

  • CaMKII regulates the strength of the epithelial barrier

    Shiomi R, Shigetomi K, Inai T, Sakai M, Junichi Ikenouchi

    SCIENTIFIC REPORTS   5   2015.8

     More details

    Language:English  

    DOI: 10.1038/srep13262

  • Sphingomyelin clustering is essential for the formation of microvilli. Reviewed International journal

    Journal of Cell Science   126 ( 16 )   3585 - 3592   2013.8

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1242/jcs.122325.

  • Role of lipids in the organization of tight junction

    Ikenouchi, J; Shigetomi, K

    MICROSCOPY   2024.9   ISSN:2050-5698 eISSN:2050-5701

     More details

  • Fluorescent Solvatochromic Probes for Long-Term Imaging of Lipid Order in Living Cells Invited Reviewed International journal

    @Takuya Tanaka, Atsushi Matsumoto, @Andery S Klymchenko, @Eiji Tsurumaki, Junichi Ikenouchi, @Gen-Ichi Konishi

    Advanced Science   11 ( 17 )   e2309721   2024.3   eISSN:2198-3844

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1002/advs.202309721

    Web of Science

    Scopus

    PubMed

  • Cytoplasmic zoning in membrane blebs

    Fujii, Y; Ikenouchi, J

    JOURNAL OF BIOCHEMISTRY   175 ( 2 )   133 - 140   2024.2   ISSN:0021-924X eISSN:1756-2651

     More details

    Language:English   Publisher:Journal of Biochemistry  

    Blebs are membrane structures formed by the detachment of the plasma membrane from the underlying actin cytoskeleton. It is now clear that a wide variety of cells, including cancer cells, actively form blebs for cell migration and cell survival. The expansion of blebs has been regarded as the passive ballooning of the plasma membrane by an abrupt increase in intracellular pressure. However, recent studies revealed the importance of ‘cytoplasmic zoning’, i.e. local changes in the hydrodynamic properties and the ionic and protein content of the cytoplasm. In this review, we summarize the current understanding of the molecular mechanisms behind cytoplasmic zoning and its role in bleb expansion.

    DOI: 10.1093/jb/mvad084

    Web of Science

    Scopus

    PubMed

  • Accumulation of annexin A2 and S100A10 prevents apoptosis of apically delaminated, transformed epithelial cells

    Ito, S; Kuromiya, K; Sekai, M; Sako, H; Sai, K; Morikawa, R; Mukai, Y; Ida, Y; Anzai, M; Ishikawa, S; Kozawa, K; Shirai, T; Tanimura, N; Sugie, K; Ikenouchi, J; Ogawa, M; Naguro, I; Ichijo, H; Fujita, Y

    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA   120 ( 43 )   e2307118120   2023.10   ISSN:0027-8424 eISSN:1091-6490

     More details

    Language:English   Publisher:Proceedings of the National Academy of Sciences of the United States of America  

    In various epithelial tissues, the epithelial monolayer acts as a barrier. To fulfill its function, the structural integrity of the epithelium is tightly controlled. When normal epithelial cells detach from the basal substratum and delaminate into the apical lumen, the apically extruded cells undergo apoptosis, which is termed anoikis. In contrast, transformed cells often become resistant to anoikis and able to survive and grow in the apical luminal space, leading to the formation of multilayered structures, which can be observed at the early stage of carcinogenesis. However, the underlying molecular mechanisms still remain elusive. In this study, we first demonstrate that S100A10 and ANXA2 (Annexin A2) accumulate in apically extruded, transformed cells in both various cell culture systems and murine epithelial tissues in vivo. ANXA2 acts upstream of S100A10 accumulation. Knockdown of ANXA2 promotes apoptosis of apically extruded RasV12-transformed cells and suppresses the formation of multilayered epithelia. In addition, the intracellular reactive oxygen species (ROS) are elevated in apically extruded RasV12 cells. Treatment with ROS scavenger Trolox reduces the occurrence of apoptosis of apically extruded ANXA2-knockdown RasV12 cells and restores the formation of multilayered epithelia. Furthermore, ROS-mediated p38MAPK activation is observed in apically delaminated RasV12 cells, and ANXA2 knockdown further enhances the p38MAPK activity. Moreover, the p38MAPK inhibitor promotes the formation of multilayered epithelia of ANXA2-knockdown RasV12 cells. These results indicate that accumulated ANXA2 diminishes the ROS-mediated p38MAPK activation in apically extruded transformed cells, thereby blocking the induction of apoptosis. Hence, ANXA2 can be a potential therapeutic target to prevent multilayered, precancerous lesions.

    DOI: 10.1073/pnas.2307118120

    Web of Science

    Scopus

    PubMed

  • Accumulation of annexin A2 and S100A10 prevents apoptosis of apically delaminated, transformed epithelial cells Reviewed International journal

    Shoko Ito, Keisuke Kuromiya, Miho Sekai, Hiroaki Sako, Kazuhito Sai, Riho Morikawa, Yohei Mukai, Yoko Ida, Moe Anzai, Susumu Ishikawa, Kei Kozawa, Takanobu Shirai, Nobuyuki Tanimura, Kenta Sugie, Junichi Ikenouchi, Motoyuki Ogawa, Isao Naguro, Hidenori Ichijo, Yasuyuki Fujita

    Proc Natl Acad Sci U S A   2023.10

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1073/pnas.2307118120.

  • Backward multiplex coherent anti-Stokes Raman (CARS) spectroscopic imaging with electron CCD camera

    Murakami, Y; Oshimura, MY; Klement, WJN; Oda, A; Sakamoto, R; Yakabe, M; Matsumoto, A; Oketani, R; Leproux, P; Ikenouchi, J; Browne, WR; Kano, H

    OPTICS CONTINUUM   2 ( 9 )   2044 - 2054   2023.9   eISSN:2770-0208

     More details

    Publisher:Optics Continuum  

    A multiplex CARS imaging system, equipped with an EM-CCD camera, was developed to improve the sensitivity of backward CARS imaging in biological analysis using an inverted microscope. The signal-to-noise ratio was improved by a factor of ca. 3 compared to a conventional CCD mode through the use of EM gain. When imaging epithelial cells in the backward CARS configuration, intracellular organelles such as lipid droplets and nuclei were spectroscopically identified with an exposure time of only 100 ms/pixel.

    DOI: 10.1364/OPTCON.497869

    Web of Science

    Scopus

  • タイトジャンクション形成においてコレステロールの集積が果たす役割の解明

    重富 健太, 小野 由美子, 松沢 健司, 池ノ内 順一

    脂質生化学研究   65   213 - 215   2023.5   ISSN:0285-1520

     More details

    Language:Japanese   Publisher:日本脂質生化学会  

    上皮細胞に存在するタイトジャンクション(TJ)と呼ばれる構造は、上皮細胞のバリア機能を担う。TJは、細胞接着分子クローディンが、裏打ちタンパク質ZOと結合を介して形質膜上で集積することで形成されるという仮説が広く受け入れられてきた。この仮説は、アドヘレンスジャンクション(AJ)と呼ばれるカドヘリンを介した別の接着機構からの類推に基づいており、その妥当性は検証されていない。私たちは、以前にTJ領域にコレステロールが高度に集積していることを報告した(Shigetomi et al.J.Cell Biol.,2018)。本研究では、クローディンの集積において、ZOタンパク質との結合ではなく、コレステロールに富む膜ドメインとの相互作用が重要であることを見出した。クローディンの発現を全て消失させた細胞において、ZOタンパク質と結合しないクローディン変異体を発現させた場合でも依然としてTJが形成される一方で、コレステロールに富む膜ドメインとの相互作用に必要なパルミトイル化修飾を消失させたクローディン変異体はTJを形成できないことが明らかになった。以上の結果から、TJの形成機構は、AJの形成機構とは異なり、コレステロールの集積したドメインがTJ形成において重要な役割を果たしていること、また、ZOタンパク質が細胞接着領域にコレステロールを集積させる機能を有していることが明らかになった。(著者抄録)

  • Cholesterol-rich domain formation mediated by ZO proteins is essential for tight junction formation

    Shigetomi, K; Ono, Y; Matsuzawa, K; Ikenouchi, J

    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA   120 ( 8 )   e2217561120   2023.2   ISSN:0027-8424 eISSN:1091-6490

     More details

    Language:English   Publisher:Proceedings of the National Academy of Sciences of the United States of America  

    Tight junctions (TJs) are cell-adhesion structures responsible for the epithelial barrier. We reported that accumulation of cholesterol at the apical junctions is required for TJ formation [K. Shigetomi, Y. Ono, T. Inai, J. Ikenouchi, J. Cell Biol. 217, 2373–2381 (2018)]. However, it is unclear how cholesterol accumulates and informs TJ formation—and whether cholesterol enrichment precedes or follows the assembly of claudins in the first place. Here, we established an epithelial cell line (claudin-null cells) that lacks TJs by knocking out claudins. Despite the lack of TJs, cholesterol normally accumulated in the vicinity of the apical junctions. Assembly of claudins at TJs is thought to require binding to zonula occludens (ZO) proteins; however, a claudin mutant that cannot bind to ZO proteins still formed TJ strands. ZO proteins were however necessary for cholesterol accumulation at the apical junctions through their effect on the junctional actomyosin cytoskeleton. We propose that ZO proteins not only function as scaffolds for claudins but also promote TJ formation of cholesterol-rich membrane domains at apical junctions.

    DOI: 10.1073/pnas.2217561120

    Web of Science

    Scopus

    PubMed

  • A Clockwork Bleb: cytoskeleton, calcium, and cytoplasmic fluidity

    Ikenouchi, J; Aoki, K

    FEBS JOURNAL   289 ( 24 )   7907 - 7917   2022.12   ISSN:1742-464X eISSN:1742-4658

     More details

    Language:English   Publisher:FEBS Journal  

    When the plasma membrane (PM) detaches from the underlying actin cortex, the PM expands according to intracellular pressure and a spherical membrane protrusion called a bleb is formed. This bleb retracts when the actin cortex is reassembled underneath the PM. Whereas this phenomenon seems simple at first glance, there are many interesting, unresolved cell biological questions in each process. For example, what is the membrane source to enlarge the surface area of the PM during rapid bleb expansion? What signals induce actin reassembly for bleb retraction, and how is cytoplasmic fluidity regulated to allow rapid membrane deformation during bleb expansion? Furthermore, emerging evidence indicates that cancer cells use blebs for invasion, but little is known about how molecules that are involved in bleb formation, expansion, and retraction are coordinated for directional amoeboid migration. In this review, we discuss the molecular mechanisms involved in the regulation of blebs, which have been revealed by various experimental systems.

    DOI: 10.1111/febs.16220

    Web of Science

    Scopus

    PubMed

  • Coordinated changes in cell membrane and cytoplasm during maturation of apoptotic bleb Invited Reviewed International journal

    #Aoki, Kana; #Satoi, Shinsuke; #Harada, Shota; Uchida, Seiichi; Iwasa, Yoh; Ikenouchi, Junichi

    MOLECULAR BIOLOGY OF THE CELL   31 ( 8 )   833 - 844   2020.4

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1091/mbc.E19-12-0691

  • Cell Adhesion Structures in Epithelial Cells Are Formed in Dynamic and Cooperative Ways Reviewed

    Kenta Shigetomi, Junichi Ikenouchi

    BioEssays   41 ( 7 )   2019.7

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1002/bies.201800227

  • Cell surface flip-flop of phosphatidylserine is critical for PIEZO1-mediated myotube formation Reviewed

    Masaki Tsuchiya, Yuji Hara, Masaki Okuda, Karin Itoh, Ryotaro Nishioka, Akifumi Shiomi, Kohjiro Nagao, Masayuki Mori, Yasuo Mori, Junichi Ikenouchi, Ryo Suzuki, Motomu Tanaka, Tomohiko Ohwada, Junken Aoki, Motoi Kanagawa, Tatsushi Toda, Yosuke Nagata, Ryoichi Matsuda, Yasunori Takayama, Makoto Tominaga, Masato Umeda

    Nature communications   9 ( 1 )   2018.12

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1038/s41467-018-04436-w

  • Adherens junctions influence tight junction formation via changes in membrane lipid composition Reviewed

    Kenta Shigetomi, Yumiko Ono, Tetsuichiro Inai, Junichi Ikenouchi

    Journal of Cell Biology   217 ( 7 )   2373 - 2381   2018.7

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1083/jcb.201711042

  • α-Catenin Controls the Anisotropy of Force Distribution at Cell-Cell Junctions during Collective Cell Migration Reviewed

    23 ( 12 )   3447 - 3456   2018.6

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    Adherens junctions (AJs) control epithelial cell behavior, such as collective movement and morphological changes, during development and in disease. However, the molecular mechanism of AJ remodeling remains incompletely understood. Here, we report that the conformational activation of α-catenin is the key event in the dynamic regulation of AJ remodeling. α-catenin activates RhoA to increase actomyosin contractility at cell-cell junctions. This leads to the stabilization of activated α-catenin, in part through the recruitment of the actin-binding proteins, vinculin and afadin. In this way, α-catenin regulates force sensing, as well as force transmission, through a Rho-mediated feedback mechanism. We further show that this is important for stable directional alignment of multiple cells during collective cell movement by both experimental observation and mathematical modeling. Taken together, our findings demonstrate that α-catenin controls the establishment of anisotropic force distribution at cell junctions to enable cooperative movement of the epithelial cell sheet. Collective cell movement requires multicellular coordination. Matsuzawa et al. show that anisotropic distribution of activated α-catenin is necessary to establish directional coordination of collectively migrating epithelial cells. α-catenin activation achieves this by modifying lateral cell adhesions through protein recruitment and activation of F-actin polymerization signaling.

    DOI: 10.1016/j.celrep.2018.05.070

  • Regulation of the epithelial barrier by post-translational modifications of tight junction membrane proteins Reviewed

    Kenta Shigetomi, Junichi Ikenouchi

    Journal of Biochemistry   163 ( 4 )   265 - 272   2018.4

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1093/jb/mvx077

  • Roles of membrane lipids in the organization of epithelial cells Old and new problems Reviewed

    Junichi Ikenouchi

    Tissue Barriers   6 ( 2 )   1 - 8   2018.4

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1080/21688370.2018.1502531

  • Emphatic visualization of sphingomyelin-rich domains by inter-lipid FRET imaging using fluorescent sphingomyelins Reviewed

    Masanao Kinoshita, Hikaru Ano, Michio Murata, Kenta Shigetomi, Junichi Ikenouchi, Nobuaki Matsumori

    Scientific Reports   7 ( 1 )   2017.12

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1038/s41598-017-16361-x

  • Membrane bleb A seesaw game of two small GTPases Reviewed

    Junichi Ikenouchi, Kana Aoki

    Small GTPases   8 ( 2 )   85 - 89   2017.4

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1080/21541248.2016.1199266

  • How do cells sense actin cortex-free membrane? Reviewed

    Junichi Ikenouchi

    Cell Cycle   15 ( 20 )   2687 - 2688   2016.10

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1080/15384101.2016.1204860

  • A RhoA and Rnd3 cycle regulates actin reassembly during membrane blebbing Reviewed

    Kana Aoki, Fumiyo Maeda, Tomoya Nagasako, Yuki Mochizuki, Seiichi Uchida, Junichi Ikenouchi

    Proceedings of the National Academy of Sciences of the United States of America   113 ( 13 )   E1863 - E1871   2016.3

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1073/pnas.1600968113

  • DAAM1 stabilizes epithelial junctions by restraining WAVE complex-dependent lateral membrane motility Reviewed

    Tamako Nishimura, Shoko Ito, Hiroko Saito, Sylvain Hiver, Kenta Shigetomi, Junichi Ikenouchi, Masatoshi Takeichi

    Journal of Cell Biology   215 ( 4 )   559 - 573   2016.1

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1083/jcb.201603107

  • CaMKII regulates the strength of the epithelial barrier Reviewed

    Ryo Shiomi, Kenta Shigetomi, Tetsuichiro Inai, Masami Sakai, Junichi Ikenouchi

    Scientific Reports   5   2015.8

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1038/srep13262

  • Targeting cholesterol in a liquid-disordered environment by theonellamides modulates cell membrane order and cell shape. International journal

    Arita Y, Nishimura S, Ishitsuka R, Kishimoto T, Junichi Ikenouchi, Ishii K, Umeda M, Matsunaga S, Kobayashi T, Yoshida M

    Chemistry and Biology   21 ( 22 )   604 - 610   2015.5

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: doi: 10.1016/j.chembiol.2015.04.011.

  • Targeting cholesterol in a liquid-disordered environment by theonellamides modulates cell membrane order and cell shape Reviewed

    Yuko Arita, Shinichi Nishimura, Reiko Ishitsuka, Takuma Kishimoto, Junichi Ikenouchi, Kumiko Ishii, Masato Umeda, Shigeki Matsunaga, Toshihide Kobayashi, Minoru Yoshida

    Cell Chemical Biology   22 ( 5 )   604 - 610   2015.5

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.chembiol.2015.04.011

  • EPLIN is a crucial regulator for extrusion of RasV12-transformed cells.

    Atsuko Ohoka, Mihoko Kajita, Junichi Ikenouchi, Yuta Yako, Sho Kitamoto, Shunsuke Kon, Masaya Ikegawa, Takashi Shimada, Susumu Ishikawa, Yasuyuki Fujita

    Journal of Cell Science   128 ( 4 )   781 - 789   2015.2

     More details

    Language:English  

    DOI: 10.1242/jcs.163113

  • EPLIN is a crucial regulator for extrusion of RasV12- transformed cells Reviewed

    Atsuko Ohoka, Mihoko Kajita, Junichi Ikenouchi, Yuta Yako, Sho Kitamoto, Shunsuke Kon, Masaya Ikegawa, Takashi Shimada, Susumu Ishikawa, Yasuyuki Fujita

    Journal of Cell Science   128 ( 4 )   781 - 789   2015.1

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1242/jcs.163113

  • Tricellulin regulates junctional tension of epithelial cells at tricellular contacts through Cdc42.

    Journal of Cell Science   127   4201 - 4212   2014.10

     More details

    Language:English  

    DOI: 10.1242/jcs.150607

  • Tricellulin regulates junctional tension of epithelial cells at tricellular contacts through Cdc42 Reviewed

    Yukako Oda, Tetsuhisa Otani, Junichi Ikenouchi, Mikio Furuse

    Journal of Cell Science   127 ( 19 )   4201 - 4212   2014.1

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1242/jcs.150607

  • Sphingomyelin clustering is essential for the formation of microvilli Reviewed

    Junichi Ikenouchi, Megumi Hirata, Shigenobu Yonemura, Masato Umeda

    Journal of Cell Science   126 ( 16 )   3585 - 3592   2013.7

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1242/jcs.122325

  • Upregulated function of mitochondria-associated ER membranes in Alzheimer disease Reviewed

    Estela Area-Gomez, Maria Del Carmen Lara Castillo, Marc D. Tambini, Cristina Guardia-Laguarta, Ad J C De Groof, Moneek Madra, Junichi Ikenouchi, Masato Umeda, Thomas D. Bird, Stephen L. Sturley, Eric A. Schon

    EMBO Journal   31 ( 21 )   4106 - 4123   2012.10

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1038/emboj.2012.202

  • Lipid polarity is maintained in absence of tight junctions Reviewed

    Junichi Ikenouchi, Mayu Suzuki, Kazuaki Umeda, Kazutaka Ikeda, Ryo Taguchi, Tetsuyuki Kobayashi, Satoshi B. Sato, Toshihide Kobayashi, Donna B. Stolz, Masato Umeda

    Journal of Biological Chemistry   287 ( 12 )   9525 - 9533   2012.3

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1074/jbc.M111.327064

  • Phosphorylation state regulates the localization of Scribble at adherens junctions and its association with E-cadherin-catenin complexes Reviewed International journal

    Yoshihara, Ken, Junichi Ikenouchi, Izumi, Yasushi, Akashi, Masaya, Tsukita, Shoichiro, Furuse, Mikio

    EXPERIMENTAL CELL RESEARCH   317 ( 4 )   413 - 422   2011.2

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.yexcr.2010.12.004

    Other Link: http://www.sciencedirect.com/science/article/pii/S0014482710005628

  • Phosphorylation state regulates the localization of Scribble at adherens junctions and its association with E-cadherin-catenin complexes Reviewed

    Ken Yoshihara, Junichi Ikenouchi, Yasushi Izumi, Masaya Akashi, Shoichiro Tsukita, Mikio Furuse

    Experimental Cell Research   317 ( 4 )   413 - 422   2011.2

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.yexcr.2010.12.004

  • LSR defines cell corners for tricellular tight junction formation in epithelial cells Reviewed

    Sayuri Masuda, Yukako Oda, Hiroyuki Sasaki, Junichi Ikenouchi, Tomohito Higashi, Masaya Akashi, Eiichiro Nishi, Mikio Furuse

    Journal of Cell Science   124 ( 4 )   548 - 555   2011.2

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1242/jcs.072058

  • FRMD4A regulates epithelial polarity by connecting Arf6 activation with the PAR complex Reviewed

    Junichi Ikenouchi, Masato Umeda

    Proceedings of the National Academy of Sciences of the United States of America   107 ( 2 )   748 - 753   2010.1

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1073/pnas.0908423107

  • Loss of occludin affects tricellular localization of tricellulin Reviewed

    Junichi Ikenouchi, Hiroyuki Sasaki, Sachiko Tsukita, Mikio Furuse, Shoichiro Tsukita

    Molecular Biology of the Cell   19 ( 11 )   4687 - 4693   2008.11

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1091/mbc.E08-05-0530

  • Defining the roles of β-catenin and plakoglobin in LEF/T-cell factor-dependent transcription using β-catenin/plakoglobin-null F9 cells Reviewed

    28 ( 2 )   825 - 835   2008.1

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    β-Catenin functions as a transcriptional regulator in Wnt signaling. Its function is regulated by a specific destruction system. Plakoglobin is a close homologue of β-catenin in mammalian cells and is regulated in a similar fashion. When β-catenin or plakoglobin is exogenously expressed in cells, endogenous β-catenin is stabilized, which complicates estimation of the transcriptional activities of exogenously expressed proteins. To facilitate the design of experiments aimed at investigating the transcriptional activities of β-catenin and plakoglobin, we utilized F9 cells in which we knocked out endogenous β-catenin and/or plakoglobin by gene deletion and exogenously expressed wild-type and mutant β-catenin and/or plakoglobin. We show that C-terminally deleted β-catenin, but not plakoglobin, has a strong dominant-negative eifect on transcription without altering the nuclear accumulation of β-catenin. Moreover, we show that Wnt-3a activation of LEF/T-cell factor (TCF)-dependent transcription depends on β-catenin but not on plakoglobin. Using chimeras of β-catenin and plakoglobin, we demonstrate that plakoglobin has the potential to function in transcriptional regulation but is not responsible for Wnt-3a signaling in F9 cells. Our data show that preferential nuclear accumulation of β-catenin is not necessarily linked to its transcriptional activity. We also clearly demonstrate that plakoglobin is insufficient for LEF/TCF-dependent transcriptional activation by Wnt-3a in F9 cells.

    DOI: 10.1128/MCB.02375-06

  • Molecular mechanisms in the formation of discrete apical and basolateral membrane domains in polarized epithelial cells Reviewed

    Junichi Ikenouchi

    Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme   52 ( 14 )   1863 - 1870   2007.11

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

  • Requirement of ZO-1 for the formation of belt-like adherens junctions during epithelial cell polarization Reviewed

    Junichi Ikenouchi, Kazuaki Umeda, Sachiko Tsukita, Mikio Furuse, Shoichiro Tsukita

    Journal of Cell Biology   176 ( 6 )   779 - 786   2007.3

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1083/jcb.200612080

  • ZO-1 and ZO-2 Independently Determine Where Claudins Are Polymerized in Tight-Junction Strand Formation Reviewed

    Kazuaki Umeda, Junichi Ikenouchi, Sayaka Katahira-Tayama, Kyoko Furuse, Hiroyuki Sasaki, Mayumi Nakayama, Takeshi Matsui, Sachiko Tsukita, Mikio Furuse, Shoichiro Tsukita

    Cell   126 ( 4 )   741 - 754   2006.8

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.cell.2006.06.043

  • Tricellulin constitutes a novel barrier at tricellular contacts of epithelial cells Reviewed

    Junichi Ikenouchi, Mikio Furuse, Kyoko Furuse, Hiroyuki Sasaki, Sachiko Tsukita, Shoichiro Tsukita

    Journal of Cell Biology   171 ( 6 )   939 - 945   2005.12

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1083/jcb.200510043

  • Apical membrane and junctional complex formation during simple epithelial cell differentiation of F9 cells Reviewed

    Satoshi Komiya, Masayuki Shimizu, Junichi Ikenouchi, Shigenobu Yonemura, Takeshi Matsui, Yoshitaka Fukunaga, Huijie Liu, Fumio Endo, Shoichiro Tsukita, Akira Nagafuchi

    Genes to Cells   10 ( 11 )   1065 - 1080   2005.11

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1111/j.1365-2443.2005.00899.x

  • Regulation of tight junctions during the epithelium-mesenchyme transition Direct repression of the gene expression of claudins/occludin by Snail Reviewed

    Junichi Ikenouchi, Miho Matsuda, Mikio Furuse, Shoichiro Tsukita

    Journal of Cell Science   116 ( 10 )   1959 - 1967   2003.5

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1242/jcs.00389

  • Embryonic hydromyelia Cystic dilatation of the lumbosacral neural tube in human embryos Reviewed

    Junichi Ikenouchi, Chigako Uwabe, Tomoko Nakatsu, Mizuki Hirose, Kohei Shiota

    Acta Neuropathologica   103 ( 3 )   248 - 254   2002.12

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1007/s00401-001-0465-9

▼display all

Presentations

  • Roles of membrane lipids in tight junction formation Invited International conference

    Junichi Ikenouchi

    Tight Junctions: from Structure and Development to Therapeutics  2023.6 

     More details

    Event date: 2023.6

    Language:English   Presentation type:Oral presentation (general)  

    Country:Switzerland  

  • Reciprocal Regulation of AJ and TJ During the Assembly of Apical Adhesion Complex International conference

    Gordon Research Conference "Cell Contact and Adhesion" 2019  2019.6 

     More details

    Event date: 2019.6

    Language:English   Presentation type:Oral presentation (general)  

    Country:Switzerland  

  • 上皮間葉転換における細胞膜脂質の質的変化の意義について

    池ノ内 順一, 塩見 僚, 重富 健太

    BMB2015(第38回日本分子生物学会年会、第88回日本生化学会大会 合同大会)  2015.12 

     More details

    Language:English   Presentation type:Oral presentation (general)  

    Venue:神戸   Country:Japan  

  • How are tight junctions different from other cell adhesion structures? Invited International conference

    Junichi Ikenouchi

    Tight Junctions Workshop  2023.11 

     More details

    Event date: 2023.11

    Language:English   Presentation type:Oral presentation (invited, special)  

    Country:Germany  

  • タイトジャンクション形成における細胞膜脂質の役割

    重富健太、池ノ内順一

    第96回日本生化学会  2023.11 

     More details

    Event date: 2023.10 - 2023.11

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Country:Japan  

  • アメーバ運動を支える細胞質ゾーニングの分子機構 Invited International conference

    池ノ内順一

    第23回日本蛋白質科学会年会  2023.7 

     More details

    Event date: 2023.7

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:名古屋   Country:Japan  

  • Cholesterol-rich domain formation mediated by ZO proteins is essential for tight junction formation

    2023.6 

     More details

    Event date: 2023.6

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Country:Japan  

  • mTORC2はスフィンゴミエリン輸送を促進することにより、低浸透圧ストレスによる細胞死を抑制する

    小野由美子、松沢健司、池ノ内順一

    第45回日本分子生物学会年会  2022.12 

     More details

    Event date: 2022.11 - 2022.12

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:千葉   Country:Japan  

  • Roles of membrane lipids in the organization of cell-cell adhestion structure. Invited International conference

    Junichi Ikenouchi

    2022.9 

     More details

    Event date: 2022.9

    Language:English   Presentation type:Symposium, workshop panel (public)  

    Country:Japan  

  • Roles of membrane lipids in tight junction formation Invited International conference

    Junichi Ikenouchi

    2021.12 

     More details

    Event date: 2021.12

    Language:English   Presentation type:Oral presentation (general)  

    Country:Japan  

  • Roles of membrane lipids in tight junction formation Invited International conference

    Junichi Ikenouchi

    4th International TJ Conference  2021.9 

     More details

    Event date: 2021.9

    Language:English   Presentation type:Oral presentation (general)  

    Country:Japan  

  • ブレブの動的な振る舞いを制御する分子機構

    #青木佳南、池ノ内順一

    第73回日本細胞生物学会大会  2021.7 

     More details

    Event date: 2021.6 - 2021.7

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:オンライン   Country:Japan  

  • Reciprocal regulation of Adherens Junction and Tight Junction during the assembly of apical adhesion complex

    2020.12 

     More details

    Event date: 2020.12

    Language:English   Presentation type:Symposium, workshop panel (public)  

    Country:Japan  

  • Molecular mechanisms underlying dynamic behavior of membrane blebs Invited

    2020.10 

     More details

    Event date: 2020.10

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  • Molecular mechanisms underlying dynamic behavior of membrane blebbing Invited

    2020.8 

     More details

    Event date: 2020.8

    Language:English   Presentation type:Oral presentation (general)  

    Country:Japan  

  • 上皮細胞に存在する細胞膜構造の形成メカニズム

    池ノ内順一

    第91回日本生化学会大会  2018.9 

     More details

    Event date: 2018.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  • Elucidation of the roles of membrane lipids in the progression of epithleial-mesenchymal transition Invited International conference

    2013.11 

     More details

    Event date: 2013.11

    Language:English   Presentation type:Oral presentation (general)  

    Country:France  

  • 細胞骨格タンパク質による細胞膜脂質の分布・動態の制御機構の解明

    前田史世, 塩見僚, 太田 安隆, 池ノ内 順一

    第36回日本分子生物学会年会  2013.12 

     More details

    Language:Japanese  

    Venue:神戸   Country:Japan  

    細胞膜を構成している膜タンパク質や脂質は膜上で区画化され存在している。細胞膜には区画化を担うDiffusion Barrierが存在すると考えられている。特に明確な細胞膜構造を示すDiffusion Barrierの例として、上皮細胞のタイトジャンクションや神経細胞のaxon initial segment等が挙げられる。これらは、いずれも細胞の極性の形成・維持や細胞の機能発現において必要不可欠な構造である。しかしながらDiffusion Barrierの分子的実体はほとんど明らかになっていない。これまでの研究により、Diffusion Barrierの形成に、細胞膜を裏打ちするアクチンフィラメントが関与していることが示唆されている。細胞内部の構造であるアクチンフィラメントが、どのようにして細胞膜の脂質分子の動態を制御しうるかについては明らかになっていない。本研究において、我々はアクチンフィラメントを架橋し網目構造を形成させるタンパク質フィラミンの発現を消失させた細胞を用いて、細胞膜の直下に存在するアクチンフィラメントの有無が細胞膜脂質の分布や動態にどのような影響をもたらすかについて解析を行った。本発表では、現在までの観察結果とそれらを踏まえた考察について紹介する。

  • 細胞膜脂質による微絨毛形成の制御機構 Invited

    池ノ内 順一

    第87回日本生化学会大会  2014.10 

     More details

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:京都   Country:Japan  

  • 上皮細胞のバリア機能の人為的制御に向けて Invited

    池ノ内 順一

    日本薬学会第135年会  2015.3 

     More details

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:神戸   Country:Japan  

  • 鳥類始原生殖細胞の移動における圧力によるブレブ形成誘発メカニズム

    森本 愛深, 池ノ内 順一, 齋藤 大介

    日本生化学会大会プログラム・講演要旨集  2023.10  (公社)日本生化学会

     More details

    Language:Japanese  

  • 高浸透圧ストレスによってタイトジャンクションの形成量が増大する分子メカニズムの解明

    長 佑磨, 谷口 明香梨, 池ノ内 順一

    日本生化学会大会プログラム・講演要旨集  2023.10  (公社)日本生化学会

     More details

    Language:Japanese  

  • 生体膜の生物物理呼応と細胞機能制御への化学的利用 細胞間接着構造の形成における膜脂質の役割(Roles of membrane lipids in the organization of cell-cell adhestion structure)

    Ikenouchi Junichi

    生物物理  2022.8  (一社)日本生物物理学会

     More details

    Language:English  

  • 上皮組織の形態形成メカニズム-接着、極性、病態の観点から- タイトジャンクション形成における細胞膜脂質の役割

    池ノ内 順一, 重富 健太

    日本生化学会大会プログラム・講演要旨集  2023.10  (公社)日本生化学会

     More details

    Language:Japanese  

  • タイトジャンクション形成においてコレステロールの集積が果たす役割の解明

    重富 健太, 小野 由美子, 松沢 健司, 池ノ内 順一

    日本生化学会大会プログラム・講演要旨集  2023.10  (公社)日本生化学会

     More details

    Language:Japanese  

  • アクチン細胞骨格の生物学・疾患における多面性と普遍性 ブレブ形成・拡大を制御する分子機構の解明

    藤井 悠貴, 池ノ内 順一

    日本生化学会大会プログラム・講演要旨集  2023.10  (公社)日本生化学会

     More details

    Language:Japanese  

  • SOCEシステムはニワトリ始原生殖細胞の移動を駆動する膜ブレブ形成に必須である。

    森田 瑞基, 森本 愛深, 寺本 孝行, 池ノ内 順一, 熱田 勇士, 齋藤 大介

    日本生化学会大会プログラム・講演要旨集  2023.10  (公社)日本生化学会

     More details

    Language:English  

▼display all

MISC

  • Cytoplasmic zoning in membrane blebs. Reviewed

    #Yuki Fujii, @Junichi Ikenouchi

    2024.2

     More details

    Language:English   Publishing type:Article, review, commentary, editorial, etc. (scientific journal)  

    DOI: 10.1093/jb/mvad084

  • Cytoplasmic zoning in membrane blebs(タイトル和訳中)

    Fujii Yuki, Ikenouchi Junichi

    The Journal of Biochemistry   175 ( 2 )   133 - 140   2024.2   ISSN:0021-924X

     More details

    Language:English   Publisher:(公社)日本生化学会  

  • 生体膜の分子機構 リピッドワールドが先導する生命科学

    池ノ内 順一, 梅田 真郷

    化学同人   2014.12

     More details

    Language:Japanese  

  • 上皮細胞の極性形成とリン脂質

    池ノ内 順一

    医学のあゆみ「生命を支える脂質―最新の研究と臨床」   2014.4

     More details

    Language:Japanese   Publishing type:Article, review, commentary, editorial, etc. (scientific journal)  

Industrial property rights

Patent   Number of applications: 2   Number of registrations: 0
Utility model   Number of applications: 0   Number of registrations: 0
Design   Number of applications: 0   Number of registrations: 0
Trademark   Number of applications: 0   Number of registrations: 0

Professional Memberships

  • Japan Society for Cell Biology

  • The Molecular Biology Society of Japan

Committee Memberships

  • 日本細胞生物学会   副会長   Domestic

    2024.6 - Present   

      More details

    Committee type:Academic society

  • 日本細胞生物学会   選挙管理委員長   Domestic

    2022.6 - 2024.6   

  • Organizer   Domestic

    2020.5 - 2022.5   

  • 日本細胞生物学会   会計幹事   Domestic

    2020.5 - 2022.5   

  • Executive   Domestic

    2018.6 - 2020.6   

  • Councilor   Domestic

    2018.4 - 2020.4   

  • Councilor   Domestic

    2017.4 - 2019.4   

  • Councilor   Domestic

    2010.4 - 2016.3   

▼display all

Academic Activities

  • プログラム委員

    第96回日本生化学会大会  ( 福岡 ) 2023.10 - 2023.11

     More details

    Type:Competition, symposium, etc. 

  • Tissue Barriers International contribution

    2018.1 - 2022.1

     More details

    Type:Academic society, research group, etc. 

  • 文部科学省科学技術政策研究所科学技術動向研究センター ライフサイエンス分野専門調査員

    Role(s): Review, evaluation

    文部科学省  2017.4 - 2022.4

     More details

    Type:Scientific advice/Review 

  • 座長(Chairmanship)

    BMB2015(第38回日本分子生物学会年会、第88回日本生化学会大会 合同大会)  ( 神戸 ) 2015.12

     More details

    Type:Competition, symposium, etc. 

  • Scientific Reports International contribution

    2015.6 - 2020.6

     More details

    Type:Academic society, research group, etc. 

  • 文部科学省科学技術政策研究所科学技術動向研究センター ライフサイエンス分野専門調査員

    Role(s): Review, evaluation

    文部科学省  2015.4 - 2016.3

     More details

    Type:Scientific advice/Review 

  • 文部科学省科学技術政策研究所科学技術動向研究センター ライフサイエンス分野専門調査員

    Role(s): Review, evaluation

    文部科学省  2014.4 - 2015.3

     More details

    Type:Scientific advice/Review 

  • 文部科学省科学技術政策研究所科学技術動向研究センター ライフサイエンス分野専門調査員

    Role(s): Review, evaluation

    文部科学省  2013.4 - 2014.3

     More details

    Type:Scientific advice/Review 

▼display all

Research Projects

  • Visualization of changes in membrane order during membrane structure formation

    Grant number:23K18141  2023 - 2024

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Challenging Research(Exploratory)

      More details

    Authorship:Principal investigator  Grant type:Scientific research funding

    CiNii Research

  • Elucidation of roles of sphingomyelin in the formation of membrane structures in epithelial cells

    Grant number:23K23881  2022.4 - 2025.3

    Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (B)

      More details

    Grant type:Scientific research funding

    CiNii Research

  • 上皮細胞の細胞膜構造形成におけるスフィンゴミエリンの機能解明

    Grant number:22H02618  2022 - 2024

    日本学術振興会  科学研究費助成事業  基盤研究(B)

      More details

    Authorship:Principal investigator  Grant type:Scientific research funding

  • 細胞質の区画化と流動性を制御する分子機構の解明

    2021 - 2023

    創発的研究支援事業(JST)

      More details

    Authorship:Principal investigator  Grant type:Contract research

  • Development of new probes for visualization of subcellular distribution of lipids

    Grant number:21K19231  2021 - 2022

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Challenging Research(Exploratory)

    Ikenouchi Junichi

      More details

    Authorship:Principal investigator  Grant type:Scientific research funding

    Biological membranes are composed of diverse lipid molecular species, and the spatiotemporal heterogeneous distribution of these lipid species is thought to be responsible for diverse intracellular functions. In this research project, I aimed to develop tools to visualise such diversity of plasma membrane lipids and the physical properties of plasma membranes formed as admixtures of diverse lipid species. Pyrene is a solvatochromic dye and allows visualisation of the order of the membrane. However, pyrene backbone dyes are highly membrane permeable, so the signal at the plasma membrane is relatively low. In this project, I developed a probe to visualise the heterogeneity of plasma membrane properties by modifying the fluorescent solvatochromic dye of the pyrene dye and selectively localising it to the plasma membrane.

    CiNii Research

  • ヒト浸潤癌における細胞膜の質的変化の検証と細胞膜を標的とした治療法開発

    2021 - 2022

    AMED革新的先端研究開発支援事業 FORCE

      More details

    Authorship:Principal investigator  Grant type:Contract research

  • 細胞膜構造の形成に関わる脂質の機能と細胞内輸送に関する研究

    Grant number:19H03227  2019 - 2021

    日本学術振興会  科学研究費助成事業  基盤研究(B)

    池ノ内 順一

      More details

    Authorship:Principal investigator  Grant type:Scientific research funding

    上皮細胞には、外界からの栄養吸収を担う細胞膜構造である微絨毛や上皮細胞同士の結合や情報伝達の場として機能する細胞接着装置などの細胞膜構造が存在する。申請者はこれらの膜構造形成において、膜タンパク質の集合・離散の制御にスフィンゴミエリンやコレステロールなどの脂質が重要な役割を果たしていることをこれまでの研究で明らかにした。本研究提案では、上皮細胞における細胞膜構造形成を可能にする細胞内でのスフィンゴミエリンやコレステロールの機能やこれらの脂質の細胞内輸送・局在化の分子機構の解明を通して細胞膜構造の構築メカニズムの解明を目指す。

    CiNii Research

  • 数理モデルによる細胞膜ブレブの形成退縮機構の理解

    Grant number:19H04968  2019 - 2020

    日本学術振興会・文部科学省  科学研究費助成事業  新学術領域研究

      More details

    Authorship:Principal investigator  Grant type:Scientific research funding

  • 細胞膜ブレブの形成退縮に関わるシグナル伝達機構の解明

    Grant number:17H06012  2017 - 2018

    日本学術振興会・文部科学省  科学研究費助成事業  新学術領域研究

      More details

    Authorship:Principal investigator  Grant type:Scientific research funding

  • ヒドラにおける上皮細胞接着シグナルの解析

    Grant number:16K14729  2016 - 2018

    科学研究費助成事業  挑戦的萌芽研究

      More details

    Authorship:Principal investigator  Grant type:Scientific research funding

  • タイトジャンクション形成の制御機構の解明

    Grant number:16H04786  2016 - 2018

    日本学術振興会  科学研究費助成事業  基盤研究(B)

      More details

    Authorship:Principal investigator  Grant type:Scientific research funding

  • 微絨毛形成における細胞膜脂質の機能解析

    Grant number:16H01362  2016 - 2017

    日本学術振興会・文部科学省  科学研究費助成事業  新学術領域研究

      More details

    Authorship:Principal investigator  Grant type:Scientific research funding

  • 上皮間葉転換に伴って変動する脂質の同定とその機能解析

    2015 - 2018

    革新的先端研究開発支援事業 AMED-PRIME「画期的医薬品等の創出をめざす脂質の生理活性と機能の解明」

      More details

    Authorship:Principal investigator  Grant type:Contract research

  • 細胞膜のIdentityの構成的理解

    Grant number:15KT0152  2015 - 2017

    日本学術振興会  科学研究費助成事業  基盤研究(C)

      More details

    Authorship:Principal investigator  Grant type:Scientific research funding

  • 細胞膜脂質が上皮管腔構造形成において果たす役割の解明

    Grant number:26112713  2014 - 2015

    日本学術振興会・文部科学省  科学研究費助成事業  新学術領域研究

      More details

    Authorship:Principal investigator  Grant type:Scientific research funding

  • 微絨毛形成におけるスフィンゴミエリンの機能解明

    Grant number:25711012  2013 - 2016

    科学研究費助成事業  若手研究(A)

      More details

    Authorship:Principal investigator  Grant type:Scientific research funding

  • 人工細胞作出に向けた人工脂質二重膜と生体膜の違いの解明

    2012 - 2016

    戦略的創造研究推進事業 (文部科学省)

      More details

    Authorship:Principal investigator  Grant type:Contract research

  • 細胞の脂質量を維持する仕組みの解明

    Grant number:24657131  2012 - 2015

    科学研究費助成事業  挑戦的萌芽研究

      More details

    Authorship:Principal investigator  Grant type:Scientific research funding

  • 細胞膜脂質が上皮管腔構造形成において果たす役割の解明

    Grant number:24112511  2012 - 2014

    日本学術振興会・文部科学省  科学研究費助成事業  新学術領域研究

      More details

    Authorship:Principal investigator  Grant type:Scientific research funding

  • 日本国際財団 研究助成金 「上皮細胞の細胞接着に関わる脂質分子の機能解析」

    2010

      More details

    Grant type:Donation

  • 細胞膜を構成する脂質分子の同定とその新規機能の解明

    Grant number:21687016  2009 - 2013

    科学研究費助成事業  若手研究(A)

      More details

    Authorship:Principal investigator  Grant type:Scientific research funding

  • 内藤財団 特定研究助成金 「上皮細胞の細胞膜ドメインを規定する脂質の探索」

    2009

      More details

    Grant type:Donation

  • 内藤記念科学奨励金 「上皮細胞の細胞接着と細胞の極性形成維持機構の分子基盤の解明」

    2009

      More details

    Grant type:Donation

  • 細胞の極性形成に関わる膜ドメインの形成・維持機構の解明

    2007 - 2011

    戦略的創造研究推進事業 (文部科学省)

      More details

    Authorship:Principal investigator  Grant type:Contract research

▼display all

Class subject

  • 分子生物学

    2023.4 - 2023.9   First semester

  • 先端生命科学

    2023.4 - 2023.9   First semester

  • 細胞生物学

    2023.4 - 2023.9   First semester

  • 生物物理学

    2023.4 - 2023.9   First semester

  • 生物科学Ⅱ

    2022.10 - 2022.12   Fall quarter

  • Basic BiologyⅡ

    2022.10 - 2022.12   Fall quarter

  • 生物科学Ⅱ

    2022.10 - 2022.12   Fall quarter

  • Basic BiologyⅡ

    2022.10 - 2022.12   Fall quarter

  • Integrative Biology, Advanced CourseⅡ

    2022.4 - 2022.9   First semester

  • 統合生物科学特論Ⅱ

    2022.4 - 2022.9   First semester

  • 分子生物学

    2022.4 - 2022.9   First semester

  • 先端生命科学

    2022.4 - 2022.9   First semester

  • 細胞生物学

    2022.4 - 2022.9   First semester

  • Integrative Biology, Advanced CourseⅡ

    2022.4 - 2022.9   First semester

  • 統合生物科学特論Ⅱ

    2022.4 - 2022.9   First semester

  • 分子生物学

    2022.4 - 2022.9   First semester

  • 先端生命科学

    2022.4 - 2022.9   First semester

  • 細胞生物学

    2022.4 - 2022.9   First semester

  • Basic BiologyⅡ

    2021.10 - 2021.12   Fall quarter

  • Basic BiologyⅡ

    2021.10 - 2021.12   Fall quarter

  • 生物科学Ⅱ

    2021.10 - 2021.12   Fall quarter

  • 生物科学特論Ⅰ

    2021.6 - 2021.8   Summer quarter

  • Biology, Advanced CouresⅠ

    2021.6 - 2021.8   Summer quarter

  • 生物科学特論Ⅰ

    2021.6 - 2021.8   Summer quarter

  • Biology, Advanced CouresⅠ

    2021.6 - 2021.8   Summer quarter

  • 分子生物学

    2021.4 - 2021.9   First semester

  • 先端生命科学

    2021.4 - 2021.9   First semester

  • 細胞生物学

    2021.4 - 2021.9   First semester

  • 生物物理学

    2021.4 - 2021.9   First semester

  • Special Lecture of Integrative Life Science Ⅷ

    2020.4 - 2020.9   First semester

  • 分子生物学

    2020.4 - 2020.9   First semester

  • 先端生命科学

    2020.4 - 2020.9   First semester

  • 細胞生物学

    2020.4 - 2020.9   First semester

  • 生物物理学

    2020.4 - 2020.9   First semester

  • 分子生命科学特論Ⅲ

    2019.10 - 2019.12   Fall quarter

  • 先端生命科学

    2019.4 - 2019.9   First semester

  • 細胞生物学

    2019.4 - 2019.9   First semester

  • 生物学特別講義Ⅰ

    2018.10 - 2019.3   Second semester

  • Basic Molecular Life Sciences Ⅱ

    2018.10 - 2018.12   Fall quarter

  • 分子生命科学Ⅱ

    2018.10 - 2018.12   Fall quarter

  • 統合生命科学特別講義Ⅷ

    2018.4 - 2018.9   First semester

  • 先端生命科学

    2018.4 - 2018.9   First semester

  • 細胞生物学

    2018.4 - 2018.9   First semester

  • 生物学特別講義Ⅷ

    2018.4 - 2018.9   First semester

  • 細胞生物学

    2018.4 - 2018.9   First semester

  • Special Lecture of Integrative Life Science Ⅷ

    2018.4 - 2018.6   Spring quarter

  • 統合生命科学特別講義Ⅷ

    2018.4 - 2018.6   Spring quarter

  • 細胞生物学

    2017.4 - 2017.9   First semester

  • 先端生命科学

    2017.4 - 2017.9   First semester

  • 細胞生物学

    2017.4 - 2017.9   First semester

  • 統合生命科学特別講義Ⅱ

    2017.4 - 2017.9   First semester

  • 統合生命科学特別講義Ⅱ

    2017.4 - 2017.9   First semester

  • 分子細胞生物学(細胞生物学II )

    2015.10 - 2016.3   Second semester

  • 生物学演習

    2015.10 - 2016.3   Second semester

  • 細胞生物学

    2015.4 - 2015.9   First semester

  • 分子細胞生物学基礎

    2015.4 - 2015.9   First semester

  • 細胞生物学(基幹教育)

    2015.4 - 2015.9   First semester

  • 細胞生物学I

    2014.10 - 2015.3   Second semester

  • 生物学演習

    2014.10 - 2015.3   Second semester

  • 細胞生物学II

    2014.4 - 2014.9   First semester

  • 分子細胞生物学基礎

    2014.4 - 2014.9   First semester

  • 細胞生物学(基幹教育)

    2014.4 - 2014.9   First semester

  • 細胞生物学II

    2013.10 - 2014.3   Second semester

  • 生物学演習

    2013.10 - 2014.3   Second semester

  • 生物科学Ⅱ

    2024.10 - 2024.12   Fall quarter

  • Basic BiologyⅡ

    2024.10 - 2024.12   Fall quarter

  • 細胞生物学

    2024.4 - 2024.9   First semester

  • 生物科学特別講義Ⅱ

    2024.4 - 2024.9   First semester

  • 生物物理学

    2024.4 - 2024.9   First semester

  • 分子生物学

    2024.4 - 2024.9   First semester

  • 先端生命科学

    2024.4 - 2024.9   First semester

  • Special Lecture of BiologyⅡ

    2024.4 - 2024.9   First semester

▼display all

FD Participation

  • 2023.3   Role:Participation   Title:【生物学科】大学発明の出願・権利化に関するFD

    Organizer:Undergraduate school department

  • 2022.3   Role:Participation   Title:全学FD:メンタルヘルス講演会

    Organizer:University-wide

  • 2022.3   Role:Participation   Title:【生物学科】入学者選抜試験に関するFD

    Organizer:Undergraduate school department

  • 2020.12   Role:Participation   Title:【オンライン配信】新型コロナウィルス感染拡大状況での学生のメンタルヘルス

    Organizer:[Undergraduate school/graduate school/graduate faculty]

  • 2020.12   Role:Participation   Title:【オンライン配信】新型コロナウィルス感染拡大状況での学生のメンタルヘルス

    Organizer:[Undergraduate school/graduate school/graduate faculty]

  • 2020.11   Role:Participation   Title:事件等発生時の学生対応に関するFD・SD

    Organizer:[Undergraduate school/graduate school/graduate faculty]

  • 2020.11   Role:Participation   Title:事件等発生時の学生対応に関するFD・SD

    Organizer:[Undergraduate school/graduate school/graduate faculty]

  • 2019.10   Role:Participation   Title:(生物学科FD)科研費改革後の学術研究動向について

    Organizer:Undergraduate school department

  • 2019.10   Role:Participation   Title:(生物学科FD)科研費改革後の学術研究動向について

    Organizer:Undergraduate school department

  • 2019.7   Role:Participation   Title:3ポリシーに関する全学FD ~日本学術会議分野別参照基準に基づく理学部物理学科の3ポリシー~

    Organizer:University-wide

  • 2019.7   Role:Participation   Title:3ポリシーに関する全学FD ~日本学術会議分野別参照基準に基づく理学部物理学科の3ポリシー~

    Organizer:University-wide

  • 2017.9   Role:Moderator   Title:教育FD

    Organizer:Undergraduate school department

  • 2014.4   Role:Participation   Title:平成26年度第1回全学FD

    Organizer:University-wide

▼display all

Social Activities

  • 未来の科学者委員/理学部の高大連携事業、「エクセレント スチューデント イン サイエンス育成プロジェクト」の生物学セミナー担当委員として参加して、高校生の研究指導を行った。

    2014.12

     More details

    Audience: Infants, Schoolchildren, Junior students, High school students

    Type:Seminar, workshop

  • 西南学院高等学校において、高校2年生を対象に「理学部における生命科学研究」というセミナーを行った。

    西南学院高等学校  2014.11

     More details

    Audience: Infants, Schoolchildren, Junior students, High school students

    Type:Seminar, workshop

  • 第13回九州大学理学部生物学科公開講座として、高校生と市民を対象に、「上皮細胞ってなんだろう」というタイトルでセミナーを行った。

    2014.8

     More details

    Audience: Infants, Schoolchildren, Junior students, High school students

    Type:Seminar, workshop

  • スーパーサイエンスハイスクール事業 実験・実習

    熊本県立第二高等学校  2013.10

     More details

    Audience: Infants, Schoolchildren, Junior students, High school students

    Type:Seminar, workshop

Media Coverage

  • 2023年2月22日の日刊工業新聞にて、上皮細胞のタイトジャンクション形成に関する論文(https://www.pnas.org/doi/abs/10.1073/pnas.2217561120)の記事が掲載された。 Newspaper, magazine

    日刊工業新聞  2023.2

     More details

    2023年2月22日の日刊工業新聞にて、上皮細胞のタイトジャンクション形成に関する論文(https://www.pnas.org/doi/abs/10.1073/pnas.2217561120)の記事が掲載された。

Year of medical license acquisition

  • 2003