Escherichia cryptic clade I is an emerging source of human intestinal pathogens Reviewed

Miki Okuno, Yoko Arimizu, Seina Miyahara, Yuki Wakabayashi, Yasuhiro Gotoh, Shuji Yoshino, Tetsuya Harada, Kazuko Seto, Takeshi Yamamoto, Keiji Nakamura, Tetsuya Hayashi, Yoshitoshi Ogura

BMC Biology   21 ( 1 )   2023.4

Nationwide analysis of antimicrobial resistance in pathogenic Escherichia coli strains isolated from diseased swine over 29 years in Japan Reviewed

Masahiro Kusumoto, Yukino Tamamura-Andoh, Yuna Hikoda-Kogiku, Asami Magome, Erina Okuhama, Keisuke Sato, Yoshino Mizuno, Nobuo Arai, Ayako Watanabe-Yanai, Taketoshi Iwata, Yoshitoshi Ogura, Yasuhiro Gotoh, Keiji Nakamura, Tetsuya Hayashi, Masato Akiba

Frontiers in Microbiology   14   2023.3

Dynamic changes in Shiga toxin (Stx) 1 transducing phage throughout the evolution of O26:H11 Stx-producing Escherichia coli Reviewed

Bungo Yano, Itsuki Taniguchi, Yasuhiro Gotoh, Tetsuya Hayashi, Keiji Nakamura

Scientific Reports   13 ( 1 )   2023.3

Global population structure, genomic diversity and carbohydrate fermentation characteristics of clonal complex 119 (CC119), an understudied Shiga toxin-producing E. coli (STEC) lineage including O165:H25 and O172:H25 Reviewed

Microbial Genomics   9 ( 3 )   2023.3

Alteration of a Shiga toxin-encoding phage associated with a change in toxin production level and disease severity in Escherichia coli. International journal

Tatsuya Miyata, Itsuki Taniguchi, Keiji Nakamura, Yasuhiro Gotoh, Dai Yoshimura, Takehiko Itoh, Shinichiro Hirai, Eiji Yokoyama, Makoto Ohnishi, Sunao Iyoda, Yoshitoshi Ogura, Tetsuya Hayashi

Microbial genomics   9 ( 2 )   2023.2

Escherichia coli O80 in Healthy Cattle: Absence of Shigatoxigenic and Enteropathogenic E. coli O80:H2 and (Phylo) Genomics of Non-Clonal Complex 165 E. coli O80 Reviewed

Microorganisms   11 ( 2 )   230 - 230   2023.1

Omics profiles of fecal and oral microbiota change in irritable bowel syndrome patients with diarrhea and symptom exacerbation.

Yukari Tanaka, Riu Yamashita, Junko Kawashima, Hiroshi Mori, Ken Kurokawa, Shinji Fukuda, Yasuhiro Gotoh, Keiji Nakamura, Tetsuya Hayashi, Yoshiyuki Kasahara, Yukuto Sato, Shin Fukudo

Journal of gastroenterology   57 ( 10 )   748 - 760   2022.10

Insertion Sequence (IS)-Excision Enhancer (IEE)-Mediated IS Excision from the lacZ Gene Restores the Lactose Utilization Defect of Shiga Toxin-Producing Escherichia coli O121:H19 Strains and Is Responsible for Their Delayed Lactose Utilization Phenotype Reviewed International journal

Keiji Nakamura, Kazuko Seto, Junko Isobe, Itsuki Taniguchi, Yasuhiro Gotoh, Tetsuya Hayashi

Applied and Environmental Microbiology   88 ( 16 )   e0076022   2022.8

Helicobacter cinaedi is a human-adapted lineage in the Helicobacter cinaedi/canicola/'magdeburgensis' complex. International journal

Yasuhiro Gotoh, Yuya Atsuta, Takako Taniguchi, Ruriko Nishida, Keiji Nakamura, Yoshitoshi Ogura, Naoaki Misawa, Tetsuya Hayashi

Microbial genomics   8 ( 5 )   2022.5

Global population structure of the Serratia marcescens complex and identification of hospital-adapted lineages in the complex Reviewed

Tomoyuki Ono, Itsuki Taniguchi, Keiji Nakamura, Debora Satie Nagano, Ruriko Nishida, Yasuhiro Gotoh, Yoshitoshi Ogura, Mitsuhiko P. Sato, Atsushi Iguchi, Kazunori Murase, Dai Yoshimura, Takehiko Itoh, Ayaka Shima, Damien Dubois, Eric Oswald, Akira Shiose, Naomasa Gotoh, Tetsuya Hayashi

Microbial Genomics   8 ( 3 )   2022.3

The global population structure and evolutionary history of the acquisition of major virulence factor-encoding genetic elements in Shiga toxin-producing Escherichia coli O121:H19. Reviewed International journal

Microbial genomics   7 ( 12 )   2021.12

Development of a homogeneous time-resolved FRET (HTRF) assay for the quantification of Shiga toxin 2 produced by E. coli Reviewed

Keiji Nakamura, Chikashi Tokuda, Hideyuki Arimitsu, Yoshiki Etoh, Mitsuhiro Hamasaki, Yuichiro Deguchi, Itsuki Taniguchi, Yasuhiro Gotoh, Yoshitoshi Ogura, Tetsuya Hayashi

PeerJ   9   e11871 - e11871   2021.7

Prophages integrating into prophages: A mechanism to accumulate type III secretion effector genes and duplicate Shiga toxin-encoding prophages in Escherichia coli Reviewed International journal

Keiji Nakamura, Yoshitoshi Ogura, Yasuhiro Gotoh, Tetsuya Hayashi

PLOS Pathogens   17 ( 4 )   e1009073 - e1009073   2021.4

Genetic characterization of Shigatoxigenic and enteropathogenic Escherichia coli O80:H2 from diarrhoeic and septicaemic calves and relatedness to human Shigatoxigenic E. coli O80:H2 Reviewed

Journal of Applied Microbiology   2020.7

Bordetella Dermonecrotic Toxin Is a Neurotropic Virulence Factor That Uses CaV3.1 as the Cell Surface Receptor Reviewed International journal

Shihono Teruya, Yukihiro Hiramatsu, Keiji Nakamura, Aya Fukui-Miyazaki, Kentaro Tsukamoto, Noriko Shinoda, Daisuke Motooka, Shota Nakamura, Keisuke Ishigaki, Naoaki Shinzawa, Takashi Nishida, Fuminori Sugihara, Yusuke Maeda, Yasuhiko Horiguchi

mBio   11 ( 2 )   2020.3

Escherichia coli O8:H8 Carrying a Novel Variant of the Heat-Labile Enterotoxin LT2 Gene Caused Outbreaks of Diarrhea Reviewed International journal

Kai Ishimaru, Mari Sasaki, Hiroshi Narimatsu, Yoko Arimizu, Yasuhiro Gotoh, Keiji Nakamura, Tetsuya Hayashi, Yoshitoshi Ogura

Open Forum Infectious Diseases   7 ( 1 )   ofaa021   2020.1

Differential dynamics and impacts of prophages and plasmids on the pangenome and virulence factor repertoires of Shiga toxin-producing Escherichia coli O145:H28 Reviewed

Microbial Genomics   6 ( 1 )   2020.1

O-antigen biosynthesis gene clusters of Escherichia albertii: their diversity and similarity to Escherichia coli gene clusters and the development of an O-genotyping method Reviewed International journal

Tadasuke Ooka, Kazuko Seto, Yoshitoshi Ogura, Keiji Nakamura, Atsushi Iguchi, Yasuhiro Gotoh, Mikiko Honda, Yoshiki Etoh, Tetsuya Ikeda, Wakana Sugitani, Takayuki Konno, Kimiko Kawano, Naoko Imuta, Kiyotaka Yoshiie, Yukiko Hara-Kudo, Koichi Murakami, Tetsuya Hayashi, Junichiro Nishi

Microbial Genomics   5 ( 11 )   2019.11

Comparison of the sequencing bias of currently available library preparation kits for Illumina sequencing of bacterial genomes and metagenomes Reviewed International journal

Mitsuhiko P Sato, Yoshitoshi Ogura, Keiji Nakamura, Ruriko Nishida, Yasuhiro Gotoh, Masahiro Hayashi, Junzo Hisatsune, Motoyuki Sugai, Itoh Takehiko, Tetsuya Hayashi

DNA Research   26 ( 5 )   391 - 398   2019.10

BspR/BtrA, an Anti-σ Factor, Regulates the Ability of Bordetella bronchiseptica To Cause Cough in Rats Invited Reviewed International journal

4 ( 2 )   2019.4

Genomic characterization of β-glucuronidase–positive escherichia coli O157:H7 producing Stx2a Invited Reviewed International journal

24 ( 12 )   2219 - 2227   2018.12

Amylibacter kogurei sp. Nov., a novel marine alphaproteobacterium isolated from the coastal sea surface microlayer of a marine inlet Reviewed

Shu Kuan Wong, Susumu Yoshizawa, Yu Nakajima, Marie Johanna Cuadra, Yuichi Nogi, Keiji Nakamura, Hideto Takami, Yoshitoshi Ogura, Tetsuya Hayashi, Hiroshi Xavier Chiura, Koji Hamasaki

International Journal of Systematic and Evolutionary Microbiology   68 ( 9 )   2872 - 2877   2018.9

The eukaryotic host factor 14-3-3 inactivates adenylate cyclase toxins of Bordetella bronchiseptica and B. Parapertussis, but not B. pertussis Reviewed International journal

Aya Fukui-Miyazaki, Hirono Toshima, Yukihiro Hiramatsu, Keisuke Okada, Keiji Nakamura, Keisuke Ishigaki, Naoaki Shinzawa, Hiroyuki Abe, Yasuhiko Horiguchi

mBio   9 ( 4 )   2018.7

Draft genome sequence of Saccharospirillum sp. strain MSK14-1, isolated from surface seawater collected at Aburatsubo Inlet in Japan Reviewed International journal

Masumi Hasegawa, Yu Nakajima, Shu Kuan Wong, Keiji Nakamura, Yoshitoshi Ogura, Tetsuya Hayashi, Kazuhiro Kogure, Susumu Yoshizawa

Genome Announcements   6 ( 22 )   2018.5

Response to “Standardized methods must be used to compare the properties of botulinum toxin serotypes” Reviewed International journal

61 ( 12 )   560 - 560   2017.12

Characterization of the functional activity of botulinum neurotoxin subtype B6 Reviewed

Kohda Tomoko, Nakamura Keiji, Hosomi Koji, Torii Yasushi, Kozaki Shunji, Mukamoto Masafumi

Microbiology and immunology   61 ( 11 )   482 - 489   2017.11

Complete and draft genome sequences of eight oceanic Pseudomonas aeruginosa strains Reviewed

Yohei Kumagai, Susumu Yoshizawa, Keiji Nakamura, Yoshitoshi Ogura, Tetsuya Hayashi, Kazuhiro Kogure

Genome Announcements   5 ( 44 )   2017.11

Protective effects of in vivo-expressed autotransporters against Bordetella pertussis infection Reviewed International journal

Koichiro Suzuki, Naoaki Shinzawa, Keisuke Ishigaki, Keiji Nakamura, Hiroyuki Abe, Aya Fukui-Miyazaki, Kazuyoshi Ikuta, Yasuhiko Horiguchi

MICROBIOLOGY and IMMUNOLOGY   61 ( 9 )   371 - 379   2017.9

Draft genome sequences of Tersicoccus phoenicis DSM 30849^T, isolated from a cleanroom for spacecraft assembly, and Tersicoccus sp. strain Bi-70, isolated from a freshwater lake Reviewed

Yu Nakajima, Susumu Yoshizawa, Keiji Nakamura, Yoshitoshi Ogura, Tetsuya Hayashi, Kazuhiro Kogure

Genome Announcements   5 ( 13 )   2017.1

百日咳毒素の感染における役割の分析(Analysis of roles of pertussis toxin in infection)

新居 佑規, 中村 佳司, 鈴木 孝一朗, 安倍 裕順, 新澤 直明, 堀口 安彦

日本細菌学雑誌   71 ( 1 )   74 - 74   2016.2

The bvg-repressed gene brtA, encoding biofilm-associated surface adhesin, is expressed during host infection by Bordetella bronchiseptica Reviewed International journal

Sayaka Nishikawa, Naoaki Shinzawa, Keiji Nakamura, Keisuke Ishigaki, Hiroyuki Abe, Yasuhiko Horiguchi

MICROBIOLOGY and IMMUNOLOGY   60 ( 2 )   93 - 105   2016.2

Bordetella pertussis感染症に対する新規の抗原の探索(Seeking novel antigens against Bordetella pertussis infection)

鈴木 孝一朗, 新澤 直明, 中村 佳司, 堀口 安彦

日本細菌学雑誌   71 ( 1 )   99 - 99   2016.2

Detection of genes expressed in Bordetella bronchiseptica colonizing rat trachea by in vivo expressed-tag immunoprecipitation method Reviewed International journal

Hiroyuki Abe, Shigeki Kamitani, Aya Fukui-Miyazaki, Naoaki Shinzawa, Keiji Nakamura, Yasuhiko Horiguchi

MICROBIOLOGY and IMMUNOLOGY   59 ( 5 )   249 - 261   2015.5

Polymorphisms influencing expression of dermonecrotic toxin in Bordetella bronchiseptica Reviewed International journal

Keisuke Okada, Hiroyuki Abe, Fumio Ike, Yoshitoshi Ogura, Tetsuya Hayashi, Aya Fukui-Miyazaki, Keiji Nakamura, Naoaki Shinzawa, Yasuhiko Horiguchi

PloS one   10 ( 2 )   e0116604   2015.2

Improved detection methods by genetic and immunological techniques for botulinum C/D and D/C mosaic neurotoxins Invited Reviewed International journal

Keiji Nakamura, Tomoko Kohda, Yoshiyuki Seto, Masafumi Mukamoto, Shunji Kozaki

VETERINARY MICROBIOLOGY   162 ( 2-4 )   881 - 890   2013.3

P19 embryonal carcinoma cells exhibit high sensitivity to botulinum type C and D/C mosaic neurotoxins Reviewed

Kentaro Tsukamoto, Hideyuki Arimitsu, Sadayuki Ochi, Keiji Nakamura, Yoshikazu Tanaka, Nipawan Nuemket, Koki Taniguchi, Shunji Kozaki, Takao Tsuji

MICROBIOLOGY and IMMUNOLOGY   56 ( 10 )   664 - 672   2012.10

Unique Biological Activity of Botulinum D/C Mosaic Neurotoxin in Murine Species Invited Reviewed International journal

Keiji Nakamura, Tomoko Kohda, Yuto Shibata, Kentaro Tsukamoto, Hideyuki Arimitsu, Mitsunori Hayashi, Masafumi Mukamoto, Nobuyuki Sasakawa, Shunji Kozaki

INFECTION AND IMMUNITY   80 ( 8 )   2886 - 2893   2012.8

Characterization of the monoclonal antibody response to botulinum neurotoxin type A in the complexed and uncomplexed forms Reviewed

Haiyang Zhao, Keiji Nakamura, Tomoko Kohda, Masafumi Mukamoto, Shunji Kozaki

Japanese Journal of Infectious Diseases   65 ( 2 )   138 - 145   2012.4

Structural and mutational analyses of the receptor binding domain of botulinum D/C mosaic neurotoxin Insight into the ganglioside binding mechanism Reviewed

Nipawan Nuemket, Yoshikazu Tanaka, Kentaro Tsukamoto, Takao Tsuji, Keiji Nakamura, Shunji Kozaki, Min Yao, Isao Tanaka

Biochemical and Biophysical Research Communications   411 ( 2 )   433 - 439   2011.7

Preliminary X-ray crystallographic study of the receptor-binding domain of the D/C mosaic neurotoxin from Clostridium botulinum Reviewed

Nipawan Nuemket, Yoshikazu Tanaka, Kentaro Tsukamoto, Takao Tsuji, Keiji Nakamura, Shunji Kozaki, Min Yao, Isao Tanaka

Acta Crystallographica Section F: Structural Biology and Crystallization Communications   66 ( 5 )   608 - 610   2010.4

Characterization of the D/C mosaic neurotoxin produced by Clostridium botulinum associated with bovine botulism in Japan Reviewed International journal

Nakamura Keiji, Kohda Tomoko, Umeda Kaoru, Yamamoto Hideyuki, Mukamoto Masafumi, Kozaki Shunji

VETERINARY MICROBIOLOGY   140 ( 1-2 )   147 - 154   2010.1

EHEC O121:H19の継代培養中に生じる乳糖分解性の変化に関わる遺伝的メカニズムの解明

中村佳司, 谷口愛樹, 西田留梨子, 後藤恭宏, 小椋義俊, 林哲也

第93回日本細菌学会総会  2020.2 

EHEC O121:H19の乳糖分解性に関与する遺伝因子と系統内のバリエーション

中村佳司, 谷口愛樹, 西田留梨子, 後藤恭宏, 小椋義俊, 林哲也

第23回腸管出血性大腸菌感染症研究会  2019.11 

腸管出血性大腸菌O121:H19の乳糖分解性に関与する遺伝因子の特定

中村佳司, 谷口愛樹, 西田留梨子, 後藤恭宏, 小椋義俊, 伊豫田淳, 大西真, 林哲也

第162回日本獣医学会学術集会  2019.9 

腸管出血性大腸菌O145:H28の比較ゲノム解析

中村佳司, 村瀬一典, 西田留梨子, 伊藤武彦, Mainil Jacques, 吉野修司, 磯部順子, 勢戸和子, 江藤良樹, 富永潔, 緒方喜久代, 斉藤志保子, 八柳潤, 黒木真理子, 木全恵子, 前田詠里子, 亀山光博, 成松浩志, 矢端順子, 伊豫田淳, 大西真, 大岡唯祐, 後藤恭宏, 小椋義俊, 林哲也

第69回日本細菌学会九州支部総会  2016.9 

腸管出血性大腸菌におけるプロファージ間相互作用と宿主菌進化へのインパクトの解明

Grant number：18K07116  2018 - 2020

日本学術振興会  科学研究費助成事業  基盤研究(C)

先進ゲノム解析研究推進プラットフォーム

2016.4 - 2021.3

先進ゲノム解析研究推進プラットフォーム

Grant number：16H06279  2016 - 2021

日本学術振興会  科学研究費助成事業  基盤研究(C)

長鎖DNA断片導入による、汎用実験動物に感染する百日咳菌の作出

Grant number：16K15276  2016 - 2017

日本学術振興会  科学研究費助成事業  基盤研究(C)

百日咳における発作性咳嗽に関連する菌側遺伝子の解析

Grant number：16K19124  2016 - 2017

科学研究費助成事業  若手研究(B)

百日咳における咳嗽発作の発症メカニズムの解明

Grant number：26293096  2014 - 2016

日本学術振興会  科学研究費助成事業  基盤研究(C)

気管支敗血症菌の新規病原因子の解析

Grant number：26860288  2014 - 2015

科学研究費助成事業  若手研究(B)

牛ボツリヌス症由来菌の産生する毒素の性状解析と特異診断法の確立

2011 - 2012

日本学術振興会  特別研究員