Updated on 2026/06/04

Information

 

写真a

 
INAGAKI SHIGENORI
 
Organization
Faculty of Medical Sciences Department of Basic Medicine Assistant Professor
School of Medicine Department of Medicine(Concurrent)
Title
Assistant Professor
Contact information
メールアドレス
Tel
0926426090
Profile
ライブ透明化、嗅覚情報処理、蛍光・発光イメージング

Research Areas

  • Life Science / Neuroscience-general

  • Life Science / Biophysics

Degree

  • Ph.D. (Engineering)

Research History

  • JST 創発研究者   

    2024.10 - Present

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  • Kyushu University Faculty of Medical Sciences Associate Professor (as old post name) 

    2021.4 - Present

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  • 2018-2021 日本学術振興会特別研究員PD   

Education

  • Osaka University   生命機能研究科   生命機能専攻

    2013.4 - 2018.3

  • Osaka University   工学部   応用自然科学科

    2010.4 - 2013.3

Research Interests・Research Keywords

  • Research theme: development of a tissue-clearing method

    Keyword: 組織透明化法

    Research period: 2025.4 - Present

  • Research theme: Elucidation of olfactory information processing

    Keyword: olfactory sensory neurons, calcium imaging

    Research period: 2021.5 - 2026.3

Awards

  • Best Presentation Award

    2024.12   動的脳機能創発(学変A) 領域会議 2024  

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  • 優秀ポスター賞

    2016.12   第39回日本分子生物学会年会  

  • Poster Award for Young Scientist

    2016.12   The 20th ISIR International Symposium  

Papers

  • RPE With ROCK-Mediated Epithelial-Mesenchymal Transition as a Key Contributor of Subretinal Fibrosis in AMD Reviewed

    Iori Wada, Keijiro Ishikawa, Muneo Yamaguchi, Shotaro Shimokawa, Ji Rui, Kenichiro Mori, Yoshihiro Kaizu, Mitsuru Arima, Shoji Notomi, Yusuke Murakami, Shigenori Inagaki, Yuta Kohro, Makoto Tsuda, Kensuke Shibata, Ryo Terao, Takeshi Terabayashi, Toshimasa Ishizaki, Shigeo Yoshida, Tatsuro Ishibashi, Susumu Ishida, Rajendra S Apte, Koh-Hei Sonoda, Shintaro Nakao

    Investigative Ophthalmology & Visual Science   67 ( 5 )   33 - 33   2026.5   ISSN:1552-5783

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Association for Research in Vision and Ophthalmology (ARVO)  

    DOI: 10.1167/iovs.67.5.33

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  • Isotonic and minimally invasive optical clearing media for live cell imaging ex vivo and in vivo Reviewed

    Shigenori Inagaki, Nao Nakagawa-Tamagawa, Nathan Zechen Huynh, Yuki Kambe, Rei Yagasaki, Satoshi Manita, Satoshi Fujimoto, Takahiro Noda, Misato Mori, Aki Teranishi, Hikari Takeshima, Koki Ishikawa, Yuki Naitou, Tatsushi Yokoyama, Masayuki Sakamoto, Katsuhiko Hayashi, Kazuo Kitamura, Yoshiaki Tagawa, Satoru Okuda, Tatsuo K. Sato, Takeshi Imai

    Nature Methods   23 ( 4 )   839 - 853   2026.3   ISSN:1548-7091 eISSN:1548-7105

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    Authorship:Lead author, Corresponding author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:Nature Methods  

    <jats:title>Abstract</jats:title>
    <jats:p>Tissue clearing has been widely used for fluorescence imaging of fixed tissues, but its application to live tissues has been limited by toxicity. Here we develop minimally invasive optical clearing media for fluorescence imaging of live mammalian tissues. Light scattering is minimized by adding spherical polymers with low osmolarity to the extracellular medium. A clearing medium containing bovine serum albumin (SeeDB-Live) is compatible with live cells, enabling structural and functional imaging of live tissues, such as spheroids, organoids, acute brain slices and the mouse brains in vivo. SeeDB-Live minimally affects neuronal electrophysiological properties and sensory responses in vivo, and facilitates fluorescence imaging of deep cortical layers in live animals without detectable toxicity to neurons or behavior. We further demonstrate its utility to epifluorescence voltage imaging in acute brain slices and in vivo preparations. Thus, SeeDB-Live expands both the depth and modality range of fluorescence imaging in live mammalian tissues.</jats:p>

    DOI: 10.1038/s41592-026-03023-y

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  • Dendritic compartment-specific spine formation in layer 5 neurons underlies cortical circuit maturation during adolescence Reviewed

    Ryo Egashira, Tsuyoshi Takagi, Meng-Tsen Ke, Yoshiaki Tagawa, Satoshi Fujimoto, Nao Nakagawa Tamagawa, Shigenori Inagaki, Tsuyoshi Miyakawa, Takeshi Imai

    Science Advances   12 ( 3 )   1 - 18   2026.1   eISSN:2375-2548

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Science Advances  

    The development of cognitive functions continues into adolescence. However, it is not fully understood how cortical circuitry changes during adolescence. Here, we performed a comprehensive super-resolution mapping of dendritic spines in layer 5 extratelencepharic-projecting (L5 ET) neurons in the primary somatosensory cortex in mice. In adults, the dendritic spines are highly enriched in the middle compartment of the apical dendrites (spine density “hotspot”), where dendritic calcium spikes are generated. In early development, dendritic spines are evenly distributed. During adolescence, however, the spine density increases specifically in the middle compartment of the apical dendrites in an experience-dependent manner, while other dendritic compartments show a slight reduction. Furthermore, spine accumulation at the hotspot was specifically impaired in mouse models of schizophrenia, demonstrating a link between adolescent spine formation and neuropsychiatric disorders. Our finding suggests that the dendritic compartment-specific spine formation during adolescence shapes nonlinear dendritic integration in L5 ET neurons and supports the maturation of cognitive functions.

    DOI: 10.1126/sciadv.adw8458

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  • Automated neuronal reconstruction with super-multicolour Tetbow labelling and threshold-based clustering of colour hues Reviewed

    Leiwe, M.N., Fujimoto, S., Baba, T., Moriyasu, D., Saha, B., Sakaguchi, R., Inagaki, S., Imai, T.

    Nature Communications   15 ( 1 )   5279   2024.6   ISSN:2041-1723 eISSN:2041-1723

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Nature Communications  

    Fluorescence imaging is widely used for the mesoscopic mapping of neuronal connectivity. However, neurite reconstruction is challenging, especially when neurons are densely labelled. Here, we report a strategy for the fully automated reconstruction of densely labelled neuronal circuits. Firstly, we establish stochastic super-multicolour labelling with up to seven different fluorescent proteins using the Tetbow method. With this method, each neuron is labelled with a unique combination of fluorescent proteins, which are then imaged and separated by linear unmixing. We also establish an automated neurite reconstruction pipeline based on the quantitative analysis of multiple dyes (QDyeFinder), which identifies neurite fragments with similar colour combinations. To classify colour combinations, we develop unsupervised clustering algorithm, dCrawler, in which data points in multi-dimensional space are clustered based on a given threshold distance. Our strategy allows the reconstruction of neurites for up to hundreds of neurons at the millimetre scale without using their physical continuity.

    DOI: 10.1038/s41467-024-49455-y

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  • Widespread Inhibition, Antagonism, and Synergy in Mouse Olfactory Sensory Neurons In Vivo Reviewed

    Shigenori Inagaki, Ryo Iwata, Masakazu Iwamoto, Takeshi Imai

    Cell Reports   31 ( 13 )   107814 - 107814   2020.6

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.celrep.2020.107814

  • Imaging local brain activity of multiple freely moving mice sharing the same environment. Reviewed International journal

    Shigenori Inagaki, Masakazu Agetsuma, Shinya Ohara, Toshio Iijima, Hideo Yokota, Tetsuichi Wazawa, Yoshiyuki Arai, Takeharu Nagai

    Scientific reports   9 ( 1 )   7460 - 7460   2019.5

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1038/s41598-019-43897-x

  • Fluorescence and Bioluminescence Imaging of Angiogenesis in Flk1-Nano-lantern Transgenic Mice. Reviewed International journal

    Jun Matsushita, Shigenori Inagaki, Tomomi Nishie, Tomoki Sakasai, Junko Tanaka, Chisato Watanabe, Ken-Ichi Mizutani, Yoshihiro Miwa, Ken Matsumoto, Kazuhiro Takara, Hisamichi Naito, Hiroyasu Kidoya, Nobuyuki Takakura, Takeharu Nagai, Satoru Takahashi, Masatsugu Ema

    Scientific reports   7 ( 46597 )   46597 - 46597   2017.4

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1038/srep46597

  • Genetically encoded bioluminescent voltage indicator for multi-purpose use in wide range of bioimaging Reviewed

    Shigenori Inagaki, Hidekazu Tsutsui, Kazushi Suzuki, Masakazu Agetsuma, Yoshiyuki Arai, Yuka Jinno, Guirong Bai, Matthew J. Daniels, Yasushi Okamura, Tomoki Matsuda, Takeharu Nagai

    Scientific Reports   7 ( 42398 )   2017.2

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1038/srep42398

  • Live imaging of neuronal dynamics in transparent mouse brains

    Inagaki, S; Imai, T

    NATURE METHODS   23 ( 4 )   703 - 704   2026.4   ISSN:1548-7091 eISSN:1548-7105

  • Isotonic and minimally invasive optical clearing media for live cell imaging ex vivo and in vivo

    Shigenori Inagaki, Nao Nakagawa-Tamagawa, Nathan Huynh, Yuki Kambe, Rei Yagasaki, Satoshi Manita, Satpshi Fujimoto, Takahiro Noda, Misato Mori, Aki Teranishi, Hikari Takeshima, Yuki Naito, Tatsushi Yokoyama, Masayuki Sakamoto, Katsuhiko Hayashi, Kazuo Kitamura, Yoshiaki Tagawa, Satoru Okuda, Tatsuo Sato, Takeshi Imai

    bioRxiv   2024.9

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    Authorship:Lead author, Corresponding author  

    DOI: 10.1101/2024.09.13.612584

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  • Automated neuronal reconstruction with super-multicolour fluorescence imaging

    Marcus N. Leiwe, Satoshi Fujimoto, Toshikazu Baba, Daichi Moriyasu, Biswanath Saha, Richi Sakaguchi, Shigenori Inagaki, Takeshi Imai

    2022.10

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    Language:Others   Publisher:Cold Spring Harbor Laboratory  

    ABSTRACT

    Fluorescence imaging is widely used for the mesoscopic mapping of neuronal connectivity. However, neurite reconstruction is challenging, especially when neurons are densely labelled. Here we report a strategy for the fully automated reconstruction of densely labelled neuronal circuits. Firstly, we established stochastic “super-multicolour” labelling with up to seven different fluorescent proteins using the Tetbow method. With this method, each neuron was labelled with a unique combination of fluorescent proteins, which were then imaged and separated by linear unmixing. We also established an automated neurite reconstruction pipeline based on the quantitative analysis of multiple dyes (QDyeFinder). To classify colour combinations, we used a newly developed unsupervised clustering algorithm, dCrawler, in which data points in multi-dimensional space were clustered based on a given threshold distance. Our new strategy allows for the reconstruction of neurites for up to hundreds of neurons at a millimetre scale without manual tracing.

    DOI: 10.1101/2022.10.20.512984

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  • In vivo Optical Access to Olfactory Sensory Neurons in the Mouse Olfactory Epithelium Reviewed

    Shigenori Inagaki, Ryo Iwata, Takeshi Imai

    bio-protocol   11 ( 12 )   2021.6

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    Language:Others   Publishing type:Research paper (scientific journal)  

    In vivo Optical Access to Olfactory Sensory Neurons in the Mouse Olfactory Epithelium

    DOI: 10.21769/bioprotoc.4055

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Books

  • 実験医学 特集1:全身全細胞解析に挑む シングルセル×空間オミクス×透明化で見る生命像 (生きた組織の透明化とライブイメージングへの応用)

    稲垣成矩, 今井猛

    羊土社  2025.12 

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  • 実験医学別冊 発光イメージング実験ガイド

    稲垣 成矩, 永井 健治

    羊土社  2019.9 

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    Language:Others  

    実験医学別冊 発光イメージング実験ガイド

  • 実験医学増刊 生きてるものは全部観る! イメージングの選び方・使い方100+

    稲垣成矩, 揚妻正和, 永井健治

    羊土社  2018.12 

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    実験医学増刊 生きてるものは全部観る! イメージングの選び方・使い方100+

Presentations

  • Isotonic and minimally invasive optical clearing media for live cell imaging ex vivo and in vivo International conference

    Shigenori Inagaki, Nao Nakagawa-Tamagawa, Nathan Zechen Huynh, Yuki Kambe, Rei Yagasaki, Satoshi Manita, Satoshi Fujimoto, Takahiro Noda, Misato Mori, Aki Teranishi, Hikari Takeshima, Yuki Naitou, Tatsushi Yokoyama, Masayuki Sakamoto, Katsuhiko Hayashi, Kazuo Kitamura, Yoshiaki Tagawa, Satoru Okuda, Tatsuo K. Sato, Takeshi Imai

    Neuronal circuits (Cold Spring Harbor Laboratory)   2026.3 

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    Event date: 2026.3

    Presentation type:Poster presentation  

  • ライブイメージングのための透明化試薬の開発 Invited

    稲垣 成矩

    第51回ExCELLSセミナー  2026.2 

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    Event date: 2026.2

    Presentation type:Public lecture, seminar, tutorial, course, or other speech  

  • 大規模計測が見出した嗅覚情報処理の非線形性

    稲垣 成矩

    第二回異分野交流会  2026.2 

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    Event date: 2026.2

  • Isotonic and minimally invasive optical clearing media for live cell imaging ex vivo and in vivo International conference

    Shigenori Inagaki, Nao Nakagawa-Tamagawa, Nathan Zechen Huynh, Yuki Kambe, Rei Yagasaki, Satoshi Manita, Satoshi Fujimoto, Takahiro Noda, Misato Mori, Aki Teranishi, Hikari Takeshima, Koki Ishikawa, Yuki Naitou, Tatsushi Yokoyama, Masayuki Sakamoto, Katsuhiko Hayashi, Kazuo Kitamura, Yoshiaki Tagawa, Satoru Okuda, Tatsuo K. Sato, Takeshi Imai

    56th International Symposium of the National Institute for Physiological Sciences Focus on Biophotonics and Neuroscience  2026.1 

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    Event date: 2026.1

    Presentation type:Poster presentation  

  • ライブイメージングのための透明化試薬の開発 Invited

    稲垣 成矩

    第 5 回 日本神経化学会 若手 KYOUEN   2026.1 

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    Event date: 2026.1

  • Isotonic and minimally invasive optical clearing media for live cell imaging ex vivo and in vivo

    稲垣 成矩

    動的脳機能創発 領域会議 2025  2025.12 

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    Event date: 2025.12

    Presentation type:Poster presentation  

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  • Isotonic and minimally invasive optical clearing media for live cell imaging ex vivo and in vivo

    稲垣 成矩

    生理学研究所シナプス研究会 2025  2025.10 

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    Event date: 2025.10

    Presentation type:Poster presentation  

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  • Minimally invasive optical clearing media for live cell imaging ex vivo and in vivo.

    Shigenori Inagaki

    The 63rd Annual Meeting of the Biophysical Society of Japan (BSJ2025)  2025.9 

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    Event date: 2025.9

    Presentation type:Symposium, workshop panel (nominated)  

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  • Isotonic and minimally invasive optical clearing media for live cell imaging ex vivo and in vivo

    Shigenori Inagaki

    JST-CREST多細胞若手の会 2025  2025.9 

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    Event date: 2025.9

    Presentation type:Poster presentation  

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  • Minimally invasive optical clearing media for fluorescence imaging of live tissues ex vivo and in vivo.

    Shigenori Inagaki, Nao Tamagawa-Nakagawa, Yuki Kambe, Rei Yagasaki, Aki Teranishi, Misato Miyagawa, Hikari Takeshima, Satoshi Fujimoto, Yuki Naito, Tatsushi Yokoyama, Masayuki Sakamoto, Keisuke Ito, Hideki Enomoto, Katsuhiko Hayashi, Yoshiaki Tagawa, Satoru Okuda, Tatsuo K. Sato, Takeshi Imai

    Gordon Research Seminar, Dendrites: Molecules, Structure and Function  2025.3 

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    Event date: 2025.3

    Presentation type:Poster presentation  

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  • Minimally invasive optical clearing media for fluorescence imaging of live tissues ex vivo and in vivo

    Shigenori Inagaki, Nao Tamagawa-Nakagawa, Yuki Kambe, Rei Yagasaki, Aki Teranishi, Misato Miyagawa, Hikari Takeshima, Satoshi Fujimoto, Yuki Naito, Tatsushi Yokoyama, Masayuki Sakamoto, Keisuke Ito, Hideki Enomoto, Katsuhiko Hayashi, Yoshiaki Tagawa, Satoru Okuda, Tatsuo K. Sato, Takeshi Imai

    Gordon Research Conference, Dendrites: Dendritic Mechanisms in Neuronal, Circuit and Systems Computations  2025.3 

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    Event date: 2025.3

    Presentation type:Poster presentation  

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  • ライブイメージングのための透明化試薬の開発 Invited

    稲垣 成矩

    第51回日本神経内分泌学会学術集会  2025.10 

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    Event date: 2025

    Language:Japanese   Presentation type:Symposium, workshop panel (nominated)  

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  • Minimally invasive optical clearing media for fluorescence imaging of live tissues ex vivo and in vivo.

    Shigenori Inagaki, Nao Tamagawa-Nakagawa, Yuki Kambe, Rei Yagasaki, Aki Teranishi, Misato Miyagawa, Hikari Takeshima, Satoshi Fujimoto, Yuki Naito, Keisuke Ito, Hideki Enomoto, Katsuhiko Hayashi, Yoshiaki Tagawa, Satoru Okuda, Tatsuo K. Sato, Takeshi Imai

    NEUROSCIENCE 2024 - Society for Neuroscience  2024.10 

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    Event date: 2024.10

    Presentation type:Poster presentation  

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  • SeeDB-Live: minimally invasive optical clearing media for fluorescence imaging of live tissue ex vivo and in vivo. NEURO2024

    Shigenori Inagaki, Nao Tamagawa-Nakagawa, Yuki Kambe, Rei Yagasaki, Aki Teranishi, Misato Miyagawa, Hikari Takeshima, Satoshi Fujimoto, Yuki Naito, Keisuke Ito, Hideki Enomoto, Katsuhiko Hayashi, Yoshiaki Tagawa, Satoru Okuda, Tatsuo K. Sato, Takeshi Imai

    CREST多細胞若手の会  2024.8 

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    Event date: 2024.8

    Presentation type:Poster presentation  

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  • SeeDB-Live: minimally invasive optical clearing media for fluorescence imaging of live tissue ex vivo and in vivo

    Shigenori Inagaki, Nao Tamagawa-Nakagawa, Yuki Kambe, Rei Yagasaki, Aki Teranishi, Misato Miyagawa, Hikari Takeshima, Satoshi Fujimoto, Yuki Naito, Keisuke Ito, Hideki Enomoto, Katsuhiko Hayashi, Yoshiaki Tagawa, Satoru Okuda, Tatsuo K. Sato, Takeshi Imai

    NEURO2024  2024.7 

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    Event date: 2024.7

    Presentation type:Poster presentation  

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  • SeeDB-Live: minimally invasive optical clearing media for fluorescence imaging of live tissue ex vivo and in vivo.

    Shigenori Inagaki, Nao Tamagawa-Nakagawa, Yuki Kambe, Rei Yagasaki, Aki Teranishi, Misato Miyagawa, Hikari Takeshima, Satoshi Fujimoto, Yuki Naito, Keisuke Ito, Hideki Enomoto, Katsuhiko Hayashi, Yoshiaki Tagawa, Satoru Okuda, Tatsuo K. Sato, Takeshi Imai

    21st International Union for Pure and Applied Biophysics and 62nd Biophysics Society of Japan (IUPAB2024)  2024.6 

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    Event date: 2024.6

    Presentation type:Poster presentation  

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  • NIG Symposium 2020

    Inagaki S, Iwata R, Imai T

    ISOT2020 

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    Event date: 2020.8

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  • Widespread inhibition and modulation in mouse olfactory sensory neurons in vivo

    Inagaki S, Iwata R, Imai T

    The 43rd Annual Meeting of the Japan Neuroscience Society 

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    Event date: 2020.7 - 2020.8

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  • Widespread inhibitory responses in the mouse olfactory sensory neurons in vivo Invited

    Shigenori Inagaki, Ryo Iwata, Takeshi Imai

    The 18th International Symposium on Molecular and Neural Mechanisms of Taste and Olfactory Perception(ISMNTOP2019) 

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    Event date: 2019.11

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  • 嗅神経細胞における抑制性匂い応答

    稲垣 成矩, 岩田 遼, 今井 猛

    第70回 西日本生理学会 

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    Event date: 2019.7

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  • 自由行動マウスの脳活動計測を可能にする化学発光膜電位センサーの開発 Invited

    稲垣成矩

    蛋白研セミナー「膜タンパク質の構造ダイナミクス」 

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    Event date: 2016.5

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  • 複数の光遺伝学操作と組み合わせたイメージングと薬剤スクリーニングの可能性を広げる発光膜電位指示薬の開発

    稲垣成矩, 揚妻正和, 松田知己, 新井由之, 白貴蓉, 神野有香, 筒井秀和, Matthew Daniels, 岡村康司, 永井健治

    第38回日本分子生物学会年会 

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    Event date: 2015.12

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  • 複数の光遺伝学操作と組み合わせたイメージングと薬剤スクリーニングの可能性を広げる発光膜電位指示薬の開発

    稲垣 成矩, 松田 知己, 揚妻正和, 新井 由之, 白貴蓉, 神野 有香, 筒井 秀和, 岡村 康史, 永井健治

    第53回日本生物物理学会年会 

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    Event date: 2015.9

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  • 複数の光遺伝学操作と組み合わせたイメージングと薬剤スクリーニングの可能性を広げる化学発光膜電位指示薬の開発 Invited

    稲垣成矩, 松田知己, 揚妻正和, 新井由之, 白貴蓉, 神野有香, 筒井秀和, 岡村康司, 永井健治

    平成27年度生理学研究所研究会「生体シグナルダイナミクス」 

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    Event date: 2015.9

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  • Genetically-encoded chemiluminescent voltage indicator applicable in millisecond voltage phenomena

    Inagaki,S, Matsuda,T, Arai,Y, Jinno,Y, Tsutsui,H, Okamura,Y, Nagai,T

    CaBP19 

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    Event date: 2015.5 - 2015.6

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  • Genetically-encoded chemiluminescent voltage indicator applicable in conjunction with multiple optogenetic tools Invited

    Inagaki,S, Matsuda,T, Arai,Y, Jinno,Y, Tsutsui,H, Okamura,Y, Nagai,T

    Next Generation Sensor Devices for a Healthier, Safer Society International Networking Workshop 

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    Event date: 2014.7

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  • Widespread inhibition and modulation in the mouse olfactory sensory neurons in vivo

    Shigenori Inagaki

    NIG Symposium 2020  2020.12 

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  • 哺乳類の多細胞組織や器官をex vivoで生きたまま解析可能な透明化法の開発

    稲垣成矩

    【CREST多細胞】領域班会議  2023.1 

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  • Allosteric modulations in odor mixture responses

    Kohei Fukata, Shigenori Inagaki, Takeshi Imai

    NEURO2022  2022.6 

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    Allosteric modulations in odor mixture responses

  • Non-synaptic mechanisms mediate global desensitization of olfactory sensory neurons in vivo

    Shigenori Inagaki, Takeshi Imai

    NEURO2022  2022.6 

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    Non-synaptic mechanisms mediate global desensitization of olfactory sensory neurons in vivo

  • 非シナプス性相互作用を介した嗅覚順応の多細胞同調機構

    稲垣成矩, 今井猛

    【臨界期生物学】領域班会議  2022.8 

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  • Chronic calcium imaging of olfactory neurons in mice

    Shigenori Inagaki, Takeshi Imai

    第44回日本神経科学大会 CJK第1回国際会議  2021.7 

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    Chronic calcium imaging of olfactory neurons in mice

  • Global adaptation in the mouse olfactory sensory neurons in vivo

    Shigenori Inagaki

    研究会「哺乳類脳の機能的神経回路の構築メカニズム」Symposium  2021.12 

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    Global adaptation in the mouse olfactory sensory neurons in vivo

  • 腸内感覚を担う迷走神経のin vivo カルシウムイメージング

    武島光里, 藤本聡志, 稲垣成矩, 今井猛

    第72回西日本生理学会  2021.11 

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    Language:Others  

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  • 非シナプス性相互作用を介した嗅覚順応の多細胞同調機構

    稲垣成矩, 今井猛

    精神・神経疾患領域/マルチセンシング連携推進ワークショップ 

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    Country:Other  

  • 非シナプス性相互作用を介した嗅覚順応の多細胞同調機構

    稲垣成矩, 今井猛

    【臨界期生物学】領域班会議  2022.8 

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  • 非シナプス性相互作用を介した嗅覚順応の多細胞同調機構

    稲垣成矩, 今井猛

    精神・神経疾患領域/マルチセンシング連携推進ワークショップ  2022.5 

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  • 腸内感覚を担う迷走神経のin vivoカルシウムイメージング

    武島 光里, 藤本 聡志, 稲垣 成矩, 今井 猛

    日本生理学雑誌  2022.2  (一社)日本生理学会

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    Language:Japanese  

  • 嗅神経細胞における多様な匂い応答は嗅覚受容体に結合するアゴニストとアロステリックエフェクターの組み合わせによって決まる

    深田 幸平, 稲垣 成矩, 今井 猛

    日本生理学雑誌  2023.5  (一社)日本生理学会

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    Language:Japanese  

  • 哺乳類の多細胞組織や器官を対象にしたex vivoライブ透明化法の開発

    稲垣成矩, 今井猛

    多細胞CREST若手の会2023  2023.9 

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  • 哺乳類の多細胞組織や器官をex vivoで生きたまま解析可能な透明化法の開発

    稲垣成矩

    【CREST多細胞】領域班会議  2023.1 

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  • ライブイメージングのための透明化試薬の開発

    稲垣成矩

    第 6 回これからの神経回路研究の会  2024.2 

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    Presentation type:Oral presentation (general)  

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  • Non-synaptic mechanisms mediate global desensitization of olfactory sensory neurons in vivo

    Shigenori Inagaki, Takeshi Imai

    NEURO2022  2022.6 

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  • Combinatorial orthosteric and allosteric interactions of an odorant receptor

    Shigenori Inagaki, Kohei Fukata, Biswanath Saha, Takeshi Imai

    ISMNTOP2023 

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  • Combinatorial allosteric interactions at odorant receptors for odor mixture coding

    Kohei FUKATA, Shigenori INAGAKI, Biswanath SAHA, Takeshi IMAI

    NEUROSCIENCE2023  2023.11 

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  • Allosteric modulations in odor mixture responses

    Kohei Fukata, Shigenori Inagaki, Takeshi Imai

    NEURO2022  2022.6 

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  • Minimally invasive optical clearing media for fluorescence imaging of live tissues ex vivo and in vivo

    Shigenori Inagaki

    動的脳機能創発 領域会議  2024.12 

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  • Minimally invasive optical clearing media for fluorescence imaging of live tissues ex vivo and in vivo Invited

    Shigenori Inagaki

    APPW 2025  2025.3 

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  • Isotonic and minimally invasive optical clearing media for live cell imaging ex vivo and in vivo International conference

    Shigenori Inagaki, Nao Tamagawa-Nakagawa, Yuki Kambe, Rei Yagasaki, Aki Teranishi, Misato Miyagawa, Hikari Takeshima, Satoshi Fujimoto, Yuki Naito, Tatsushi Yokoyama, Masayuki Sakamoto, Keisuke Ito, Hideki Enomoto, Katsuhiko Hayashi, Yoshiaki Tagawa, Satoru Okuda, Tatsuo K. Sato, Takeshi Imai

    The 2025 Cold Spring Harbor Asia Conference  2025.4 

  • Isotonic and minimally invasive optical clearing media for live cell imaging ex vivo and in vivo

    Shigenori Inagaki, Nao Tamagawa-Nakagawa, Yuki Kambe, Rei Yagasaki, Aki Teranishi, Misato Miyagawa, Hikari Takeshima, Satoshi Fujimoto, Yuki Naito, Tatsushi Yokoyama, Masayuki Sakamoto, Keisuke Ito, Hideki Enomoto, Katsuhiko Hayashi, Yoshiaki Tagawa, Satoru Okuda, Tatsuo K. Sato, Takeshi Imai

    The 2025 Cold Spring Harbor Asia Conference  2025.4 

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  • 生体シグナル情報の定量化に基づく多細胞間相互作用の時空間的理解 ex vivo/in vivoライブイメージングのための等張かつ低侵襲な透明化培地の開発(Spatial-temporal understanding of multicellular interactions based on quantification of biological signal information Isotonic and minimally invasive optical clearing media for live cell imaging ex vivo and in vivo)

    Inagaki Shigenori, Tamagawa Nao, Huynh Nathan, Kambe Yuki, Yagasaki Rei, Manita Satoshi, Fujimoto Satoshi, Noda Takahiro, Naito Yuki, Hayashi Katsuhiko, Kitamura Kazuo, Tagawa Yoshiaki, Okuda Satoru, Sato Tatsuo, Imai Takeshi

    The Journal of Physiological Sciences  2025.3  (一社)日本生理学会

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    Language:English  

  • 大規模計測が見出した嗅覚情報処理の非線形性

    稲垣 成矩

    第二回異分野交流会  2026.2 

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  • 区画・領域依存的生命現象とその可視化 ライブイメージングのための透明化試薬の開発(Compartment- and Region-Dependent Biological Phenomena and Visualization Minimally invasive optical clearing media for live cell imaging ex vivo and in vivo)

    Inagaki Shigenori

    生物物理  2025.8  (一社)日本生物物理学会

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  • ライブ透明化法を用いた大規模イメージング技術基盤の構築

    Shigenori Inagaki, Nao Tamagawa-Nakagawa, Yuki Kambe, Rei Yagasaki, Aki Teranishi, Misato Miyagawa, Hikari Takeshima, Satoshi Fujimoto, Yuki Naito, Tatsushi Yokoyama, Masayuki Sakamoto, Keisuke Ito, Hideki Enomoto, Katsuhiko Hayashi, Yoshiaki Tagawa, Satoru Okuda, Tatsuo K. Sato, Takeshi Imai

    第四回創発融合の場  2025.7 

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  • ライブ透明化法を用いた大規模イメージング技術基盤の構築

    稲垣 成矩

    第二回 創発の場  2025.11 

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  • ライブイメージングのための透明化試薬の開発 Invited

    稲垣 成矩

    第 5 回 日本神経化学会 若手 KYOUEN  2026.1 

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  • ライブイメージングのための透明化試薬の開発 Invited

    稲垣 成矩

    第51回ExCELLSセミナー  2026.2 

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  • Isotonic and minimally invasive optical clearing media for live cell imaging ex vivo and in vivo

    Shigenori Inagaki, Nao Tamagawa-Nakagawa, Yuki Kambe, Rei Yagasaki, Aki Teranishi, Misato Miyagawa, Hikari Takeshima, Satoshi Fujimoto, Yuki Naito, Tatsushi Yokoyama, Masayuki Sakamoto, Keisuke Ito, Hideki Enomoto, Katsuhiko Hayashi, Yoshiaki Tagawa, Satoru Okuda, Tatsuo K. Sato, Takeshi Imai

    第48回日本神経科学大会  2025.7 

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  • Isotonic and minimally invasive optical clearing media for live cell imaging ex vivo and in vivo

    Shigenori Inagaki, Nao Nakagawa-Tamagawa, Nathan Zechen Huynh, Yuki Kambe, Rei Yagasaki, Satoshi Manita, Satoshi Fujimoto, Takahiro Noda, Misato Mori, Aki Teranishi, Hikari Takeshima, Koki Ishikawa, Yuki Naitou, Tatsushi Yokoyama, Masayuki Sakamoto, Katsuhiko Hayashi, Kazuo Kitamura, Yoshiaki Tagawa, Satoru Okuda, Tatsuo K. Sato, Takeshi Imai

    56th International Symposium of the National Institute for Physiological Sciences Focus on Biophotonics and Neuroscience  2026.1 

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  • Isotonic and minimally invasive optical clearing media for live cell imaging ex vivo and in vivo

    Shigenori Inagaki, Nao Nakagawa-Tamagawa, Nathan Zechen Huynh, Yuki Kambe, Rei Yagasaki, Satoshi Manita, Satoshi Fujimoto, Takahiro Noda, Misato Mori, Aki Teranishi, Hikari Takeshima, Yuki Naitou, Tatsushi Yokoyama, Masayuki Sakamoto, Katsuhiko Hayashi, Kazuo Kitamura, Yoshiaki Tagawa, Satoru Okuda, Tatsuo K. Sato, Takeshi Imai

    Neuronal circuits (Cold Spring Harbor Laboratory)  2026.3 

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  • ライブイメージングのための透明化試薬の開発

    稲垣 成矩

    第 5 回 日本神経化学会 若手 KYOUEN  2026.1 

  • Isotonic and minimally invasive optical clearing media for live cell imaging ex vivo and in vivo International conference

    Shigenori Inagaki, Nao Nakagawa-Tamagawa, Nathan Zechen Huynh, Yuki Kambe, Rei Yagasaki, Satoshi Manita, Satoshi Fujimoto, Takahiro Noda, Misato Mori, Aki Teranishi, Hikari Takeshima, Koki Ishikawa, Yuki Naitou, Tatsushi Yokoyama, Masayuki Sakamoto, Katsuhiko Hayashi, Kazuo Kitamura, Yoshiaki Tagawa, Satoru Okuda, Tatsuo K. Sato, Takeshi Imai

    56th International Symposium of the National Institute for Physiological Sciences Focus on Biophotonics and Neuroscience  2026.1 

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MISC

  • 嗅神経細胞のin vivoカルシウムイメージングによって見いだされた多様な匂い応答様式

    稲垣成矩, 今井猛

    Aroma Research   2021.2

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  • Development of a Wireless Brain Activity Recording Method Based on Bioluminescence

    Shigenori INAGAKI, Masakazu AGETSUMA, Takeharu NAGAI

    Seibutsu Butsuri   2020.1

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    DOI: 10.2142/biophys.60.117

  • 細胞・個体イメージング用光学プローブの開発 Reviewed

    鈴木和志, 稲垣成矩, 松田知己, 永井健治

    化学工業   2019.9

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    細胞・個体イメージング用光学プローブの開発

  • 蛍光タンパク質 vi.脳活動計測のための膜電位プローブ

    稲垣成矩, 揚妻正和, 永井健治

    実験医学   2018.12

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    蛍光タンパク質 vi.脳活動計測のための膜電位プローブ

  • 蛍光・化学発光ライブイメージングの現状と展望 Reviewed

    永井健治, 鈴木和志, 稲垣成矩

    第43回組織細胞化学講習会テキスト   2018.7

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    蛍光・化学発光ライブイメージングの現状と展望

  • A novel fiber-free technique for brain activity imaging in multiple freely behaving mice

    Shigenori Inagaki, Masakazu Agetsuma, Takeharu Nagai

    Optogenetics and Optical Manipulation 2018   2018.2

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    Language:Others  

    DOI: 10.1117/12.2302396

  • Bioluminescent indicator applicable to membrane voltage recording in various excitable cell types

    Shigenori Inagaki, Takeharu Nagai

    Biomedical Imaging and Sensing Conference   2017.4

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    DOI: 10.1117/12.2267502

  • An Investigation of Effect of Bioluminescent Light on Human using Electroencephalogram

    Thammasan, N, Iwano, M, Moriyama, K, Fukui, K, Kawintiranon, K, Buatong, Y, Inagaki, S, Nagai, T, Numao, M

    Proc. The 23nd International Display Workshop in conjunction with Asia Display   2016.12

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  • Current progress in genetically encoded voltage indicators for neural activity recording

    Shigenori Inagaki, Takeharu Nagai

    Current Opinion in Chemical Biology   2016.8

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    DOI: 10.1016/j.cbpa.2016.05.023

  • 遺伝子にコードされた膜電位センサーによる神経活動計測の現状と展望 Reviewed

    稲垣成矩, 永井健治

    Drug Delivery System   2016.3

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    遺伝子にコードされた膜電位センサーによる神経活動計測の現状と展望

    DOI: 10.2745/dds.31.119

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Industrial property rights

Patent   Number of applications: 1   Number of registrations: 0
Utility model   Number of applications: 0   Number of registrations: 0
Design   Number of applications: 0   Number of registrations: 0
Trademark   Number of applications: 0   Number of registrations: 0

Professional Memberships

  • THE BIOPHYSICAL SOCIETY OF JAPAN

  • THE JAPAN NEUROSCIENCE SOCIETY

  • 日本生理学会

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  • Society for Neuroscience

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Committee Memberships

  • 生物物理学会2027年小倉年会 実行委員コアメンバー  

    2025.9 - Present   

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Academic Activities

  • CREST「多細胞」若手の会(第三回)の幹事

    Role(s): Planning, management, etc.

    2025.9

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    Type:Academic society, research group, etc. 

  • CREST「多細胞」若手の会(第二回)の幹事

    Role(s): Planning, management, etc.

    2024.8

Other

  • JST創発学内説明会 創発研究者による体験談(講演)

    2026.3

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Research Projects

  • 革新的標識・透明化技術を用いた脳機能・形態イメージング

    Grant number:25wm0625128h0001  2025.8 - 2028.3

    AMED  脳神経科学統合プログラム 個別重点課題 領域1 革新的技術・研究基盤の整備・開発・高度化 

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    Authorship:Coinvestigator(s)  Grant type:Competitive funding other than Grants-in-Aid for Scientific Research

  • ライブ透明化法を用いた大規模イメージング技術基盤の構築

    Grant number:24013642  2024.10 - 2030.4

    国立研究開発法人科学技術振興機構  創発的研究支援事業  

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    Authorship:Principal investigator  Grant type:Competitive funding other than Grants-in-Aid for Scientific Research

  • 嗅覚受容体における匂いの混合物応答モデルの確立

    Grant number:24K01702  2024 - 2027

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (B)

    稲垣 成矩

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    Authorship:Principal investigator  Grant type:Scientific research funding

    自然界の匂いは、複数種類の匂い分子から構成される。従来、匂いの混合物に対する嗅神経細胞の応答は、その構成分子に対する応答の単純な足し合わせになると考えられてきた。しかし実際には、匂いを足し合わせると、元の匂いからは想像できない匂いになることがある。本提案では、嗅覚受容体のアロステリック効果を考慮した混合物応答モデルを確立する。そして、未知の混合物に対する嗅覚受容体の応答を予測可能なプログラムの開発を目指す。これにより、混合物に対する情報処理の理解が進むのみならず、調香師の知識や経験に基づかない匂いの設計にも繋がることが期待される。

    CiNii Research

  • 哺乳類の多細胞組織や器官をex vivoで生きたまま解析可能な透明化法の開発

    Grant number:22K18373  2022.4 - 2026.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Challenging Research(Pioneering)

    稲垣 成矩

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    Authorship:Principal investigator  Grant type:Scientific research funding

    哺乳類のような複雑な生命システムを理解するためには、まずは組織・器官、そしてゆくゆくは個体全体を、単一細胞レベルの分解能で解析する必要がある。近年、組織透明化法が普及したことで、PFA固定した組織・器官においては、蛍光イメージングによる大規模な解析が可能になっている。しかし、固定標本を対象とした従来の手法では、動的な生命現象を理解することはできない。そこで本課題では、組織・器官を、生体外で“生きたまま”解析できる組織透明化法の開発を目指す。

    CiNii Research

  • Elucidation of the mechanism by which foul odors enhance the perception of smells

    Grant number:21H02140  2021 - 2023

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (B)

    Inagaki Shigenori

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    Authorship:Principal investigator  Grant type:Scientific research funding

    The allosteric effect is the phenomenon whereby the binding of a molecule to a site other than the ligand binding site causes a change in receptor activity. In this study, we examined odor responses of olfactory sensory neurons and found that odorant receptors exhibit allosteric effects. In addition, we demonstrated that the allosteric effect actually occurs at the level of the odorant receptor with in vitro reconstitution assays.

    CiNii Research

  • 行動中マウスの膜電位イメージングによる能動的嗅覚受容機構の解明

    Grant number:18J00899  2018 - 2020

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for JSPS Fellows

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    Grant type:Scientific research funding

  • 新規発光膜電位センサーを用いたマウスの運動指令形成メカニズム解明

    Grant number:16J00111  2016 - 2017

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for JSPS Fellows

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    Grant type:Scientific research funding

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Educational Activities

  • 学部学生への生理学の講義・実習および統計解析の基礎を指導している。また、大学院生への神経科学のための光学イメージング技術および顕微鏡技術の講義を行っている。研究室では、動物組織のライブイメージングに関する実験系構築を中心に、学生への研究指導を行っている。

Class subject

  • 生命科学研究法

    2024.10 - Present   Second semester

  • 分子生理

    2024.4 - Present   First semester

  • 人体構造と機能Ⅱ

    2023.4 - Present   First semester

  • 生理学実習

    2021.10 - Present   Second semester

FD Participation

  • 2022.12   Title:医学系学府教育FD「医学系大学院プログラムの進化と深化をめざして」

  • 2022.8   Title:医学部医学科・生命科学科FD「医学教育分野別評価受審の振り返りについて」

  • 2021.12   Title:医学系学府教育FD「学術論文の購読と投稿とこれから」

Social Activities

  • 学園祭において、自身の研究を一般参加者に発表した。

    大阪大学産業科学研究所  産業科学研究所  2017.4

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    Audience:General, Scientific, Company, Civic organization, Governmental agency

    Type:Other

Media Coverage

  • ‘Impossible’ Now Possible: Making Brain Tissue Transparent Internet

    Medscape  https://www.medscape.com/viewarticle/impossible-now-possible-making-brain-tissue-transparent-2026a1000fkn?form=fpf  2026.5

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    Author:Other 

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  • 生きたマウス 脳細胞を観察 Newspaper, magazine

    日本経済新聞  2026.4

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  • 生きたマウスの脳細胞を観察 九州大学が試薬、光の屈折や散乱防ぐ Internet

    日本経済新聞  https://www.nikkei.com/article/DGXZQOSG10A040Q6A310C2000000/  2026.3

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  • 生きた動物の脳 世界初の透明化 Newspaper, magazine

    日刊工業新聞  2026.3

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  • 脳組織を透明化し観察 生きた状態で、試薬開発 Newspaper, magazine

    西日本新聞  2026.3

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  • 生物組織の透明化技術進展 東大は全身、九大も「生きたまま」 Internet

    時事通信社  https://www.jiji.com/jc/article?k=2026031201191&g=soc  2026.3

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  • A new reagent makes living brains transparent for deeper, non-invasive imaging Internet

    EurekAlert!, Medical Xpress  2026.3

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  • 脳深部、生きたまま観察 九大、試薬で組織透明化 Internet

    共同通信、デイリースポーツ、神戸新聞NEXT、山陽新聞、北海道新聞、東京新聞、日本海新聞、Web東奥、琉球新報、埼玉新聞、佐賀新聞、岩手日報、中國新聞、大分合同新聞、愛媛新聞、奈良新聞、信濃毎日新聞、南日本新聞、四国新聞、千葉日報、秋田魁新報、河北新報、  https://news.jp/i/1404766539143561474?c=39550187727945729  2026.3

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  • 細胞を生きたまま透明にして観察できる試薬開発 九州大学など Internet

    NHK ONE  https://news.web.nhk/newsweb/na/na-k10015074051000 2026年3月12日  2026.3

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  • Using blood proteins to make living brains transparent Internet

    news-medical.net  2026.3

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  • Seeing the Brain in a Different Light Internet

    Genetic Engineering & Biotechnology News  2026.3

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  • Scientists can now see deep-brain activity without disrupting it Internet

    TECH EXPLORIST  2026.3

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  • New Reagent Renders Brains Transparent for Imaging Internet

    Mirage News  2026.3

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  • New tissue-clearing techniques let microscopes peer deeper into living brains Internet

    The Transmitter: Neuroscience News and Perspectives  2024.10

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  • 靴下+バニラ=チョコ? におい調和 仕組み解明

    西日本新聞  2020.7

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    靴下+バニラ=チョコ? におい調和 仕組み解明

  • 「嗅覚について、(靴下の臭い+バニラの匂い=チョコレート?)」

    多田しげおの気分爽快~朝からP.O.N  2020.7

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    「嗅覚について、(靴下の臭い+バニラの匂い=チョコレート?)」

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