Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1007/s13758-012-0027-9

Language：English   Publishing type：Research paper (scientific journal)  

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1111/j.1600-0501.2011.02163.x

Language：Japanese   Publishing type：Research paper (scientific journal)  

Language：English   Publishing type：Research paper (scientific journal)  

Language：English   Publishing type：Research paper (scientific journal)  

Language：English   Publishing type：Research paper (scientific journal)  

Language：English   Publishing type：Research paper (scientific journal)  

Language：English   Publishing type：Research paper (scientific journal)  

Language：English   Publishing type：Research paper (scientific journal)  

Language：English   Publishing type：Research paper (scientific journal)  

Language：English   Publishing type：Research paper (scientific journal)  

Language：English   Publishing type：Research paper (scientific journal)  

Language：English   Publishing type：Research paper (scientific journal)  

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1016/j.tripleo.2005.03.007

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1002/jbm.a.30110

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1111/j.1600-0501.2004.01015.x

Publishing type：Research paper (scientific journal)  

DOI： 10.1034/j.1600-0501.2002.130303.x

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1902/jop.2000.71.6.961

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1002/(SICI)1097-4636(199807)41:1<111::AID-JBM14>3.0.CO;2-Q

Language：Japanese   Publisher：Japanese Society of Oral Implantology  

DOI： 10.11237/jsoi.37.85

CiNii Research

Language：Japanese   Publisher：Japanese Society of Oral Implantology  

<p>It is generally easier to achieve favorable primary stability in mandibular dental implant cases than in maxillary cases because the mandibular bone is harder. Nevertheless, we have experienced two cases of dental implants that were displaced into the mandibular bone. We present these two cases and describe their removal and the clinical consequences.</p><p>Case 1 : A 52-year-old female patient. The implant body was displaced into the mandibular bone during placement of the implant. The displaced implant was removed with a long guide pin from the hole made during implant surgery.</p><p>Case 2 : A 35-year-old female patient. The implant body was displaced into the mandibular bone during placement of the implant using a torque wrench. In this case, a 3-D model was prepared using computed tomographic (CT) data to simulate the surgery to remove the implant body. Buccal cortical bone was cut out and the implant body was safely removed. In both cases, no sequelae such as nerve paralysis were reported.</p><p>It is widely known that the information from panoramic and CT images is important. However, it should be emphasized that the surgeonʼs own tactile sensation during preparation of the implant bed is also important. It is recommended to decide the drilling and placement protocol based on feel during the implant surgery.</p><p>A displaced implant can be removed using either a crestal approach or a lateral approach. It is important to remove the displaced implants with appropriate surgical planning using CT data at an early timing.</p>

DOI： 10.11237/jsoi.37.186

CiNii Research

Language：English   Publisher：Journal of Evidence-Based Dental Practice  

Objectives: An increasing number of studies have identified an association between oral health status and cognitive function. However, the effect of oral interventions, including oral health care, dental treatment and oral motor exercises, on cognitive function remains unclear. This systematic review examined whether oral interventions contribute to the long-term improvement of cognitive status. Methods: Four databases were searched (MEDLINE, Web of Science, Cochrane Library, and ICHUSHI Web) to identify randomized and nonrandomized controlled trial studies and prospective cohort studies from inception until 1 September 2023, published in English or Japanese. The Cochrane risk of bias tool for randomized controlled trials and the risk of bias assessment tool for nonrandomized studies were used to assess bias risk. Results: A total of 20 articles were included in the qualitative analysis; 13 articles were published in English, and 7 were published in Japanese. The implemented interventions were oral care in 8 studies, dental treatment in 8 studies, and oral motor exercise in 4 studies. One study found a significant effect on attention following oral care intervention. Some dental treatments influenced cognitive function, although a clear positive effect was not determined. In 1 study, attention and working memory improved in the chewing exercise group. Conclusions: Several studies verified the improvement effects of oral interventions, such as oral care, dental treatment, and oral motor exercise, on cognitive function or impairment. However, there was still a lack of conclusive evidence that such an intervention clearly improved cognitive function. To clarify the effects of oral interventions on cognitive function, it is necessary to examine participants, interventions, and outcome measures in detail.

DOI： 10.1016/j.jebdp.2023.101948

Web of Science

Scopus

PubMed

Language：Others   Publishing type：Research paper (scientific journal)  

This study aimed to evaluate the bioactivity of poly(ether ether ketone) (PEEK) after surface modification by persistent photoconductive strontium titanate (SrTiO3) magnetron sputtering and ultraviolet (UV) C irradiation. According to the different modifications, the PEEK specimens were randomly divided into five groups (n = 38/group): PEEK, Sr100-PEEK, Sr200-PEEK, UV/PEEK, and UV/Sr200-PEEK. Then, the specimens of Sr100-PEEK and Sr200-PEEK groups were, respectively, coated with 100 and 200 nm thickness photocatalyst SrTiO3 on the PEEK surface by magnetron sputtering. Subsequently, UV-C light photofunctionalized the specimens of PEEK and Sr200-PEEK groups to form UV/PEEK and UV/Sr200-PEEK groups. The specimens were characterized by a step meter, scanning electron microscopy (SEM), atomic force microscopy (AFM), energy dispersive X-ray spectroscopy (EDX), and a water contact angle meter. The release test of the Sr ion was performed by inductively coupled plasma mass spectrometry (ICP-MS). In vitro study, osteogenic activity (MC3T3-E1 osteoblast-like cells) and epithelial and connective tissue attachment (gingival epithelial cells GE1 and fibroblasts NIH3T3) were analyzed in five groups. Surface morphology of the specimens was changed after coating, and the Sr content on the Sr-PEEK surface was increased with increasing coating thickness. In addition, the contact angle was increased significantly after magnetron sputtering. After UV-C photofunctionalization, the content of surface elements changed and the contact angle was decreased. The release of Sr ion was sustained, and the final cumulative release amount did not exceed the safety limit. In vitro experiments showed that SrTiO3 improved the cell activity of MC3T3-E1 and UV-C irradiation further enhanced the osteogenic performance of PEEK. Besides, UV-C irradiation also significantly promoted the cell viability, development, and expression of adhesion proteins of GE1 and NIH3T3 on PEEK. The present investigation demonstrated that nano SrTiO3 coating with UV-C photofunctionalization synergistically enhanced the osteogenic properties and soft tissue sealing function of PEEK in vitro.

DOI： 10.1021/acsbiomaterials.3c01684

Web of Science

Scopus

PubMed

researchmap

Language：English   Publisher：日本大学歯学部  

年齢が20～49歳でEichner分類がAの患者を対象に、口腔機能を表す3種の指標を解析した。2020年4～11月に福岡市にある単一の開業歯科医院を受診した上記患者183名(女性133名、平均34.0±7.7歳)のデータを後ろ向きに抽出した。口腔機能の指標としては、最大咬合力(MOF)、最大舌圧(MTP)、咀嚼機能を測定した。咀嚼機能はグミゼリー(グルコラム)からのグルコース溶出濃度として計測した。口腔機能低下症の診断基準となる閾値として、MOFでは500N、MTPでは30.0kPa、咀嚼機能では100mg/dLという値を設定した。全集団における3指標の平均値はいずれも閾値より高くなった。個々の患者でみると閾値を下回った患者はMTPで最も多く30名に上り、MOFでは19名、咀嚼機能ではわずかに3名のみであった。20、30、40歳代の年齢層別で比較したが、どの口腔機能指標にも有意な差はみられなかった。また男女比較ではどの口腔機能指標も女性より男性の方が有意に高かった。2種の口腔機能指標が共に閾値を下回っていた患者は9名いた。いずれも女性患者であり、うち8名は歯列不正を伴っていた。以上のように、一部の患者では、不便を自覚していないにもかかわらず口腔機能、特にMOFとMTPの値が低下していた。歯列不正は口腔機能低下に関連していると考えられた。

Language：English   Publisher：Nihon University School of Dentistry  

<p><b>Purpose:</b> The purpose of this study was to analyze oral functions (MOF: maximum occlusal force; MTP: maximum tongue pressure; and MF: masticatory function) in Eichner A subjects aged from 20 to 49 years.</p><p><b>Methods:</b> The data of three oral functions in Eichner A patients aged from 20 to 49 years were extracted. The mean values, subjects with lower values than the thresholds, and the differences among ages, and between sexes were investigated retrospectively.</p><p><b>Results:</b> Although the mean values of these oral functions in 183 subjects were higher than the thresholds, the numbers of the subjects with lower values than the thresholds were 30 in MTP, 19 in MOF, and only three in MF. No significant effects of ages on all oral functions were identified and all functions in males were significantly higher than those in females. The subjects whose two functions were lower than the thresholds were nine females, eight of whom were associated with malalignment.</p><p><b>Conclusion:</b> Although the mean values of oral functions were higher than the thresholds, some patients showed lower values, especially in MOF and MTP, even though they were unaware of any inconvenience. Patients with malalignment might be associated with declined oral functions.</p>

DOI： 10.2334/josnusd.23-0311

Web of Science

Scopus

PubMed

CiNii Research

Language：English   Publisher：Healthcare (Switzerland)  

“Combination syndrome”, defined by Kelly in 1972, is a challenging condition observed in a patient with an edentulous maxilla and a partially edentulous mandible with only mandibular anterior teeth. “Anterior hyperfunction syndrome” is regarded as a synonym of combination syndrome and was first described in 1994. Although these terms have been well known, the definition of “anterior hyperfunction” has not been described yet. This narrative review focused on anterior hyperfunction and discussed the etiology and the clinical managements. An electronic bibliographic search for this literature review was conducted in addition to the review of our clinical cases. The previous reports indicated that combination syndrome with all five features was rarely observed. The patients with anterior hyperfunction generally showed the loss of posterior occlusal supports and the loss of vertical dimension of occlusion. To manage anterior hyperfunction, these conditions should be improved using conventional removable prostheses and implant-supported prostheses. Anterior hyperfunction is attributed to mandibular anterior teeth and some interventions for mandibular anterior teeth are required in many cases. Additionally, it must be noted that implant-supported prostheses may lead to anterior hyperfunction. In conclusion, comprehensive approaches for the remaining teeth and the prostheses will be required to manage this complex condition.

DOI： 10.3390/healthcare11222967

Web of Science

Scopus

PubMed

Language：English   Publisher：Journal of Clinical Medicine  

The aim of this retrospective cross-sectional study was to comprehensively assess masticatory function in maxillectomy patients with functioning removable prostheses. Their general and oral profiles, the measurement values of their oral functions, including masticatory function, and the history of tumor therapy were extracted from medical charts. The correlations of masticatory function with numerical data and the effects of tumor therapy-related factors on masticatory function were evaluated. In addition, a stepwise conditional logistic regression analysis was performed to identify the potential predictive factors comprehensively. The data from 55 maxillectomy patients revealed that the median value of masticatory function (138.0 mg/dL) was higher than the threshold (100.0 mg/dL) based on the concept of oral hypofunction. Moderate correlations of masticatory function with the number of remaining teeth, the number of functioning occlusal supports, and maximum occlusal force were found, as well as a weak correlation with maximum tongue pressure. These variables also showed statistically significant coefficients (p < 0.01). No significant effect of each tumor therapy-related factor on masticatory function was detected. A logistic regression analysis identified the number of functioning occlusal supports as a significant predictive factor. These results implied the crucial interactions of masticatory function with various factors and the specificities of maxillectomy patients.

DOI： 10.3390/jcm12155117

Web of Science

Scopus

PubMed

Language：English   Publishing type：Research paper (scientific journal)   Publisher：Japan Prosthodontic Society  

<p><b>Purpose:</b> The aim of this study was to review the literature on current surface modification strategies used to improve the binding efficiency of an emerging biological material, polyetheretherketone (PEEK), with bone and soft tissues.</p><p><b>Study selection:</b> This review was based on articles retrieved from PubMed, Google Scholar, Web of Science, and ScienceDirect databases. The main keywords used during the search were “polyetheretherketone (PEEK),” “implant,” “surface modification,” “biomaterials,” “bone,” “osseointegration,” and “soft tissue.”</p><p><b>Results:</b> The suitability of PEEK surface modification strategies has been critically analyzed and summarized here. Many cell and <i>in vivo</i> experiments in small animals have shown that the use of advanced modification technologies with appropriate surface modification strategies can effectively improve the surface inertness of PEEK, thereby improving its binding efficiency with bone and soft tissues.</p><p><b>Conclusions:</b> Surface modifications of PEEK have revealed new possibilities for implant treatment; however, most results are based on <i>in vitro</i> or short-term <i>in vivo</i> evaluations in small animals. To achieve a broad application of PEEK in the field of oral implantology, more <i>in vivo</i> experiments and long-term clinical evaluations are needed to investigate the effects of various surface modifications on the tissue integration ability of PEEK to develop an ideal implant material.</p>

DOI： 10.2186/jpr.JPR_D_22_00138

Web of Science

Scopus

PubMed

CiNii Research

researchmap

Language：Japanese   Publisher：Japanese Society of Oral Implantology  

<p>Oral implant treatment has become an important prosthetic option for defects. Osseointegration is important for the implant body to function in the oral cavity, but soft tissue sealing is essential for long-term functioning. The mechanism of sealing around the implant is similar in structure to the biological widths around natural teeth, but its performance is clearly lower. Due to the anatomical lack of blood flow around the implant, the amount of adhesion-related protein that intervenes histologically with the implant body is small, and epithelial cells adhere weakly to titanium. To improve the soft tissue sealing around the implant, research on the material of the implant body, the surface texture, and the cell activity of the surrounding tissue is still under way. We believe that these considerations affect the prognosis of implant treatment, and that maintenance considering soft tissue clearly extends the life of the implant.</p>

DOI： 10.11237/jsoi.36.32

CiNii Research

Language：English   Publishing type：Research paper (scientific journal)   Publisher：The Japanese Society for Dental Materials and Devices  

<p>Carbonate apatite (CO₃Ap) is a major inorganic bone component and an effective bone substitute. To clarify the function of CO₃Ap, we compared differences among CO₃Ap, hydroxyapatite (HAp), and β-tricalcium phosphate (β-TCP) by focusing on mesenchymal stem cells (MSCs) that have a role in wound healing. For <i>in vivo</i> experiments, maxillary molars were removed and the bone substitute was inserted. MSC accumulation around extraction sockets was significantly promoted in CO₃Ap and β-TCP groups. For <i>in vitro</i> experiments, MSCs were cultured with bone substitutes. The differentiation potential and amount of calcium deposition were significantly lower in CO₃Ap and HAp groups than in the β-TCP group. Increases in insulin-like growth factor-I and vascular endothelial growth factor were found only in the CO₃Ap group. CO₃Ap-filled extraction sockets accumulated MSCs, and MSCs cultured in the presence of CO₃Ap produced large amounts of growth factors. These results suggest that CO₃Ap promotes healing of tooth extraction sockets.</p>

DOI： 10.4012/dmj.2022-040

Web of Science

Scopus

PubMed

CiNii Research

researchmap

Language：English   Publishing type：Research paper (scientific journal)  

Language：English   Publisher：(公社)日本口腔インプラント学会  

researchmap

Language：English   Publisher：(一社)日本歯科理工学会  

骨補填材としての炭酸アパタイト(炭酸Ap)をハイドロキシアパタイト(HAp)およびβリン酸三カルシウム(β-TCP)と比較し、間葉系幹細胞(MSC)に注目してそれらの間にみられる差異を検討した。6週齢の雄性ラット30匹を使用してin vivo実験を施行した。上顎の第1・2大臼歯を抜歯し、抜歯窩に上記3種の骨補填材のいずれかを移植した。その3日後と7日後に深麻酔死させ各種の評価に供した。その結果、炭酸Ap群とβ-TCP群では、抜歯窩付近のMSCの集積が有意に促進された。MSCを骨補填材の存在下で培養するin vitro実験も行ったところ、炭酸Ap群とHAp群では分化能とカルシウム沈着量がβ-TCP群よりも有意に低減していた。インスリン様成長因子I(IGF-I)と血管内皮細胞増殖因子(VEGF)が増加しているという結果は炭酸Ap群のみで認められた。炭酸Apを充填した抜歯窩にはMSCが集積し、炭酸Ap存在下で培養したMSCは成長因子類を大量に産生したことから、炭酸Apは抜歯窩の治癒を促進することが示唆された。

Language：English   Publisher：mSphere  

The tongue dorsum is colonized by a stable microbiota, mostly comprising common commensal taxa. However, the predominance of each taxon varies among individuals. We hypothesized that equilibrium in the tongue microbiota is affected by exposure to butyrate in the oral fluid, which is reported to affect the growth of specific microorganisms. In this study, the bacterial composition of the tongue microbiotas of 69 male adults was determined via 16S rRNA gene sequencing to investigate its relationship to n-butyric acid concentration in oral rinse samples. The tongue microbiotas of individuals with a higher n-butyric acid level had higher relative abundances of Prevotella histicola, Veillonella atypica, and Streptococcus parasanguinis and lower relative abundances of Neisseria subflava and Porphyromonas pasteri. Subsequently, tongue microbiota samples collected from 12 adults were cultivated for 13 h in basal medium containing mucin and different concentrations of sodium butyrate (0, 0.8, 1.6, and 3.2 mM) to assess its effect on the growth of tongue microbiota organisms. The bacterial composition of the cultivated tongue microbiotas also demonstrated a significant gradual shift with an increase in sodium butyrate levels in beta-diversity analysis. N. subflava was significantly less predominant in the microbiota after cultivation with an increased addition of sodium butyrate, although no statistical difference was observed in the other aforementioned taxa. These results suggest that butyrate in the oral fluid is partially involved in the dysbiotic shift of the tongue microbiota. IMPORTANCE Oral microbial populations that are always ingested with saliva have attracted increasing attention because more oral microorganisms than previously known reach distal organs, such as the lungs and intestinal tract, thereby affecting our health. However, although such organisms are predominately derived from the tongue dorsum, the dynamics and determinants of the tongue microbiota composition remain unclear. This study demonstrated that exposure to butyrate could lead to a dysbiotic shift in the tongue microbiota using an observational epidemiological and microbiota cultivation approach. This result adds a new dimension to tongue microbiota ecology.

DOI： 10.1128/msphere.00490-22

Web of Science

Scopus

PubMed

Language：Japanese   Publisher：Japan Prosthodontic Society  

<p>Japan Prosthodontic Society (JPS) has been developing an education curriculum suitable for training board-certified prosthodontists as required by Japanese Dental Specialty Board (JDSB). The curriculum is now almost complete, and an overview of the curriculum was presented at the 131st Annual Meeting of JPS. Due to time constraints, we were not able to present some of the topics at the seminar, so we have written this article to let our members know about the contents of the education curriculum.</p><p>The curriculum design of board-certified prosthodontist system consists of a basic training program to acquire the basic knowledge and skills of a prosthodontist as a fundamental training program, which is followed by a case series-based evaluation and a competence-based evaluation from the viewpoint of the abilities that a prosthodontist should fulfill. These evaluations and the level of learning achievement are organized in the “Board-Certified Prosthodontist Training Evaluation Record”, which is a kind of practice book. This paper mainly outlines the method of filling out this evaluation record, and describes the credits and examinations required for certification as a specialist in prosthodontics accredited by JDSB.</p><p>The curriculum is currently being tested at various training institutions and will be revised from time to time to correct any inconsistencies or operational problems. We hope that it will serve as a reference for teaching young prosthodontists and for young dentists undergoing education.</p>

DOI： 10.2186/ajps.15.19

CiNii Research

Language：English   Publisher：John Wiley & Sons Australia, Ltd  

2009～2015年に包括的健康診断に参加した地域在住高齢者1240名(男性539名、平均76.6±7.1歳)を対象として、現在歯数および機能歯数と将来における自立喪失の発生率との関連について検討した。主要評価項目は自立喪失の発生(初回要支援要介護認定と定義)とした。調査項目は口腔内検査による現在歯数(1～9本、10～19本、20本以上)および機能歯数(0～19本、20本以上)、人口統計学的特徴、各種臨床変数(慢性疾患の既往、心理社会的要因)、血液栄養マーカー、身体機能、知覚的咀嚼機能などとした。最終的に1121名を解析対象とした。その結果、追跡調査期間中に205名に自立喪失が認められた。カプランマイヤー解析の結果、現在歯数および機能歯数が少ない者では、現在歯数または機能歯数が多い者と比較して、自立喪失の発生率が有意に大きかった。Cox比例ハザード分析の結果、人口統計学的特徴による調整後、現在歯数の少なさは自立喪失の発生の有意な危険因子でなかったが、機能歯数は有意な危険因子であることが示された(ハザード比:1.975)。以上から、機能歯数は現在歯数と比較して将来における自立喪失の発生率と密接に関連している可能性が示された。

Language：Others   Publishing type：Research paper (scientific journal)   Publisher：Wiley  

DOI： 10.1111/ggi.14508

Web of Science

Scopus

PubMed

researchmap

Other Link： https://onlinelibrary.wiley.com/doi/full-xml/10.1111/ggi.14508

Language：English   Publishing type：Research paper (scientific journal)  

Bone augmentation using artificial bone is an important option in dental defect prostheses. A bone substitute using carbonate apatite (CO3Ap), an inorganic component of bone, was reported to have promising bone formation and bone replacement ability. However, the osteoinductivity of artificial bone is less than autogenous bone (AB). In this study, CO3Ap with AB is demonstrated as a clinically effective bone substitute. For in vitro experiments, an osteoclast-like cell (RAW-D) was cultured in the presence of AB, CO3Ap, or both (Mix), and the number of osteoclasts was evaluated. Osteoblasts were also cultured under the same conditions, and the number of adherent cells was evaluated. For in vivo experiments, a few holes were created in the rat tibia and AB, CO3Ap, or Mix were added. At 0, 14, and 21 days, the tissue morphology of the wound area was observed, and the thickness of the cortical bone was measured. In vitro, Mix did not increase the number of osteoclasts or osteoblasts. However, in vivo, the rate of bone replacement remarkably increased with Mix on dome-shape. A bone-grafting material combining osteoinductive AB with abundant artificial bone is expected to be clinically easy to use and able to form bone.

DOI： 10.3390/ma15228100

Web of Science

Scopus

PubMed

researchmap

Language：English   Publisher：Materials  

In the intraoral scanner (IOS) impression technique for dental implants, a scanbody (SB) is connected to the implant and scanned. Poly(ether-ether-ketone) (PEEK) is a widely used material for SBs and it is recommended for single use. However, from the perspective of the Sustainable Development Goals, it is desirable to use these products multiple times. As SBs are used in patients’ mouths, proper sterilization is necessary for multiple uses. In the present study, the effect of autoclave treatment and connection/disconnection on SB deformation was investigated. The SB was connected to the implant and stereolithography (STL) data were obtained. Then, the SB was disconnected and underwent autoclave treatment, or was connected and disconnected multiple times, or underwent a combination of both processes. The results showed that there were significant differences in the distance and angle when comparing SBs before and after the autoclave treatment, but repeated connections with or without autoclave treatment had no significant impact on the measured values. The surface texture, observed with scanning electron microscopy, showed that a groove was observed on the surface of the SB, but the groove did not show major changes after 10 connection/autoclave processes. These results indicate that autoclave sterilization has some impact on SB deformation but connection/disconnection itself may not have a huge impact on SB deformation.

DOI： 10.3390/ma15217717

Web of Science

Scopus

PubMed

Language：English   Publishing type：Research paper (scientific journal)  

The clinical remount is an accurate and efficient way to reset the occlusion of delivered removable dentures if major occlusal correction is required. Although previous studies have reported that clinical remounting of existing dentures enhances patients' oral function, little subjective feedback is available. This retrospective study reports short-term changes in oral-health-related quality of life (OHRQoL) and masticatory function after clinical remounting of existing dentures. Three time points were defined: before adjustment (T0), immediately after adjustment (T1), and 1 week after adjustment (T2). The medical records of seven patients were analyzed. The mean age of participants was 77.71 years, and the mean service period of their prostheses was 9.43 months. The mean scores of the OHIP-EDENT-J questionnaire at the respective time points were 35, 21.14, and 22.14. The mean readings of masticatory function at the respective time points were 76.71, 89.29, and 111.86. Significant differences in the OHIP-EDENT-J were found between T0 and T1, and T0 and T2; and in masticatory function between T1 and T2, and T0 and T2. The results indicated that after rebalancing of the occlusion of the existing dentures, the patient-reported OHRQoL was improved immediately and maintained at least for a short time, and masticatory function was enhanced over a 1-week period.

DOI： 10.3390/healthcare10101960

Web of Science

Scopus

PubMed

researchmap

Language：English   Publisher：日本大学歯学部  

単一臼歯へのモノリシックジルコニア冠(MZC)とその対合歯の3年累積生存率を評価し、同生存率に影響している要因を解析した。2014年4月～2020年9月に当大学病院で単一臼歯へのMZC修復治療を行った患者105名(女性80名、平均64.2±11.3歳)の177冠を後方視的に組み入れた。MZC失敗の定義は、抜歯などでのMZCの撤去とした。対合歯失敗の定義は、対合歯に何らかの介入が必要になったこと(ただし、再セメント固定および咬合調整が単独で行われた例を除く)とした。観察期間は22.7±10.5ヵ月で、MZCの3年累積生存率は89.8%(95%CI 80.0～95.1%)、対合歯の同率は94.8%(95%CI 89.3～97.6%)となった。生存予測因子の候補として顎(上顎か下顎か)、歯種(小臼歯か大臼歯か)、歯髄の状態(生活か失活か)、を採り上げ、調整済みのCox比例ハザードモデルで検証した。その結果、MZC失敗と有意に関連している因子としては歯髄失活が同定された(ハザード比2.8e+9、P=0.01)。対合歯失敗の独立関連因子は対合歯自身の失活状態であった(ハザード比7.8、P=0.03)。単一臼歯MZC治療の成績は臨床的に許容できるものであったが、支台歯および対合歯が失活していることはそれぞれの治療失敗の潜在的な危険因子になっていると考えられた。

Publishing type：Research paper (scientific journal)   Publisher：Elsevier BV  

DOI： 10.1016/j.ceramint.2022.05.336

Web of Science

Scopus

researchmap

Language：Japanese   Publisher：The Japanese Society for Temporomandibular Joint  

<p>Oral appliances (OA) are widely used in daily clinical practice as a reversible and conservative therapy for masticatory muscle pain and sleep bruxism (SB). However, there is insufficient evidence for their therapeutic effect. Hence, the objectives of this randomized clinical trial were to clarify the effects of OA on masticatory muscle pain and SB activity. Sixteen patients who were referred to the TMD clinic at Kyushu University Hospital with masticatory muscle pain and SB participated in this study. They were randomly allocated to either the splint group, who wore OA during sleep, or the control group. Electromyographic activity of the masseter muscle during sleep was recorded at baseline, immediately after, and 1, 4, and 6 weeks after the insertion of OA. At baseline and 6 weeks after starting the treatment, masticatory muscle pain was evaluated by a visual analog scale and muscle palpation, and Oral Health Impact Profile-49 (OHIP-49) scores were recorded. There were statistically significant differences in the muscle palpation index and OHIP-49 total score between the OA and control groups. There was a significant reduction in SB events immediately after wearing OA, but no change at 1, 4, or 6 weeks after starting the treatment. A simple correlation between SB activity and pain parameters was not found. In conclusion, OA had positive effects on both masticatory muscle pain and SB, but effects on SB were transient.</p>

DOI： 10.11246/gakukansetsu.34.38

CiNii Research

Language：English   Publishing type：Research paper (scientific journal)  

BACKGROUND: A novel type of implant (Straumann® BLX implant) has been developed for certain stability from the mechanical and biological aspects and is expected for the implant placement in atrophic maxilla with sinus floor elevation (SFE). PURPOSE: The aim of this study was to evaluate the primary stability in the implants with different macrodesigns in an SFE simulated model. Primary stabilities defined as maximum insertion torque (MIT) and implant stability quotient (ISQ) were compared between this novel type of implant and other types. MATERIALS AND METHODS: Five types of Straumann® 10 mm length implants (Standard Plus; SP, Tapered Effect; TE, Bone Level; BL, Bone Level Tapered; BLT and BLX) and two types of Straumann® 6 mm length implants (SP short, BLX short) were used in this study. Each implant was inserted through 5 mm-thick porcine iliac crest blocks (an SFE simulated model). Primary stability was evaluated by using MIT and ISQ. RESULTS: The mean value of MIT for BLX group showed significantly higher values than SP, BL (p < 0.01), and TE (p < 0.05) groups. The mean value of ISQ for BLX group was significantly higher than the other groups (p < 0.01). The mean value of MIT and ISQ for BLX and BLX short group were significantly higher than those for SP and SP short group (p < 0.01). CONCLUSIONS: In an SFE simulated ex vivo model, BLX group showed the highest values. These results suggest that implant selection can play a crucial role in the achievement of primary stability during SFE and simultaneous implant placement.

DOI： 10.1186/s12903-022-02345-5

Web of Science

Scopus

PubMed

researchmap

Language：English   Publishing type：Research paper (scientific journal)  

Medication-related osteonecrosis of the jaw (MRONJ) is an intractable disease that is typically observed in patients with osteoporosis or tumors that have been treated with either bisphosphonate (BP) or antiangiogenic medicine. The mechanism of MRONJ pathogenesis remains unclear, and no effective definitive treatment modalities have been reported to date. Previous reports have indicated that a single injection of benidipine, an antihypertensive calcium channel blocker, in the vicinity of a tooth extraction socket promotes wound healing in healthy rats. The present study was conducted to elucidate the possibility of using benidipine to promote the healing of MRONJ-like lesions. In this study, benidipine was administered near the site of MRONJ symptom onset in a model rat, which was then sacrificed two weeks after benidipine injection, and analyzed using histological sections and CT images. The analysis showed that in the benidipine groups, necrotic bone was reduced, and soft tissue continuity was recovered. Furthermore, the distance between epithelial edges, length of necrotic bone exposed in the oral cavity, necrotic bone area, and necrotic bone ratio were significantly smaller in the benidipine group. These results suggest that a single injection of benidipine in the vicinity of MRONJ-like lesions can promote osteonecrotic extraction socket healing.

DOI： 10.3390/ph15081020

Web of Science

Scopus

PubMed

researchmap

DOI： 10.14693/jdi.v29i2.1320

Web of Science

Language：Japanese   Publisher：(一社)日本顎関節学会  

オーラルアプライアンス(OA)は,顎関節症(TMD)および睡眠時ブラキシズム(SB)の治療に広く用いられているが,そのエビデンスは不十分である。そこで本研究では,ランダム化比較試験によりOAの治療効果を調査し,TMDとSBの相互関係を調べることを目的とした。九州大学病院を受診したTMD患者のうち,咀嚼筋痛およびSBを有する者16名を対象として,OA装着群と非装着群(各8名)に無作為割付を行った。各群において6週間にわたり咀嚼筋痛の程度,口腔関連QoL(Oral Health Impact Profile-49,OHIP-49),SB(携帯型筋電図)の記録を行い,比較検討した。また,治療効果に関連する因子とそれらの関与程度を調査するため,心理学的因子とダイアリーの記録を行った。咀嚼筋痛に関して,VASによる痛みの主観的評価では群間に有意差は認められなかったが,筋圧痛スコアおよびOHIP-49スコアにおいてはOA装着群のほうが有意に改善していた。また,SBはOA装着直後において有意に減少したが,1,4,6週後には差はみられなかった。SBと咀嚼筋痛との間に相関関係は認められなかった。OA治療は,咀嚼筋痛の緩和に有効であること,また1週間程度ではあるがSBの減弱にも効果があることが明らかとなった。(著者抄録)

Language：English   Publisher：Healthcare (Switzerland)  

This retrospective study aimed to investigate the survival rate of implants from 5 to 10 years after the placement of implant-supported fixed dental prostheses (ISFDPs) and the management of implant loss in the elderly population. Elderly patients (≥65 years old) who had been treated with ISFDPs and followed up with for at least 5 years between October 2009 and March 2020 were enrolled. Patient profiles and implant-related data were extracted. The survival rate of implants up to 5 years as well as the 10-year cumulative survival rate were evaluated. The management of implant loss and prosthetic interventions were also investigated. In total, 195 patients (mean age: 70.1 ± 4.5 years old) and 687 implants (287 ISFDPs) were assessed. The 5-year survival rate was 99.0% and the 10-year cumulative survival rate was 98.1%. Seven of the eleven implants lost were lost due to peri-implantitis. Only three implants in two patients were placed after the loss of the implants; most were restored using non-invasive procedures. Two patients underwent a conversion from ISFDPs to removable prostheses. This study showed that high survival rates were observed in an elderly population with ISFDPs and that non-invasive procedures were often applied after the loss of an implant.

DOI： 10.3390/healthcare10071250

Web of Science

Scopus

PubMed

Language：English   Publisher：Diagnostics  

Although the clinical assessment of enamel thickness is important, hardly any tools exist for accurate measurements. The purpose of this study was to verify the precision of enamel thickness measurements using swept-source optical coherence tomography (SS-OCT). Human extracted maxillary central and lateral incisors were used as specimens. Twenty-eight sites were measured in each specimen. The optical path length (OPL) at each measurement site was measured on the OCT images, and enamel thickness (e1) was calculated by dividing OPL by the mean refractive index of enamel, 1.63. The specimens were then sectioned, and a light microscope was used to measure enamel thickness (e2). e1 and e2 were then compared. Measurement errors between e1 and e2 for the central and lateral incisors were 0.04 (0.02; 0.06) mm and 0.04 (0.02; 0.07) mm [median value: (25%, 75% percentile)], respectively. No significant differences between measurement sites were noted for measurement errors between e1 and e2. These results demonstrate that OCT can be used for noninvasive, accurate measurements of enamel thickness.

DOI： 10.3390/diagnostics12071634

Web of Science

Scopus

PubMed

Language：Japanese   Publisher：Japanese Society of Oral Implantology  

<p>In Japan, oral surgeons, prosthodontists, or periodontists are often in charge of implant treatment. Therefore, it is thought that a method to accurately determine the difficulty level of implant treatment, especially for treatment in areas other than</p><p>their own main field, would help to provide safe and secure implant treatment.</p><p>Currently, several tools for determining the difficulty level of implant treatment have been proposed, but there are some problems that cannot be well expressed by the various classification methods that have been used so far. For example, a single tooth defect in a mandibular molar with no problems in bone quality or quantity and no occlusal problems may be considered to be an easy case, but for an inexperienced dentist, drilling the bone itself may seem difficult. Thus, whether a case is easy or difficult depends greatly on the operator's perception and experience. Apart from such kinds of technical difficulty, which is perceived differently by different operators, there is another level of difficulty based on the general condition, the treatment site, and the individuality of the patient, and so on. High-quality evidence generally shows that the success rate of implant treatment decreases when there are problems with these items.</p><p>The Research Promotion Committee of the Japanese Society of Oral Implantology is attempting to classify the difficulty level of implant treatment, which can be called an Evidence-Based Difficulty Assessment Tool, independent of the surgeon's experience. This paper presents a trial proposal.</p>

DOI： 10.11237/jsoi.35.83

CiNii Research

Language：English   Publishing type：Research paper (scientific journal)  

BACKGROUND: Refractory jaw osteonecrosis that occurs in osteoporotic or cancer patients treated with bisphosphonates is called medication-related osteonecrosis of the jaw but its underlying mechanism is unclear. Statins, therapeutic agents for dyslipidemia, lower blood low-density lipoprotein cholesterol. Fluvastatin promotes the healing of tooth extraction sockets and reduces the risk of developing medication-related osteonecrosis of the jaw-like lesions. We used a rat model to investigate whether injecting fluvastatin at extraction sites promoted the healing of medication-related osteonecrosis of the jaw-like lesions. METHODS: Upper first molars of rats administered zoledronate and dexamethasone for 2 weeks were extracted. Two weeks after tooth extraction, rats with medication-related osteonecrosis of the jaw-like lesions (bone exposure) were included in this study. A single injection of fluvastatin was administered in the vicinity of the medication-related osteonecrosis of the jaw-like onset site in rats. RESULTS: The distance between the edges of the epithelia, the length of the necrotic bone exposed toward the oral cavity, the area of the necrotic bone, and the necrotic bone ratio were significantly smaller in the fluvastatin-administered group compared with the saline group. A single application of fluvastatin near the site of medication-related osteonecrosis of the jaw onset showed a tendency to close the epithelium, reduce necrotic bone, and form new bone, even when symptoms had already developed. CONCLUSION: This study suggests that a single topical administration of fluvastatin may be a novel treatment for medication-related osteonecrosis of the jaw.

DOI： 10.1002/JPER.21-0294

Web of Science

Scopus

PubMed

researchmap

Language：Japanese   Publishing type：Research paper (scientific journal)  

本邦では口腔外科や補綴,歯周病のいずれかに軸足を置く歯科医師がインプラント治療を担当することが多いように思われる.そのため,特に自身がメインフィールドとする領域以外の治療において正確な難易度を判定する手法があれば,安心安全なインプラント治療を提供することができると考えられる.現在,いくつかのインプラント治療難易度判定ツールが提案されているが,これまでに用いられてきた種々の分類法では難易度をうまく表現できない問題も存在する.たとえば,骨の質,量ともに問題なく咬合にも問題ない下顎臼歯部1歯中間欠損などはイージーケースといっても差し支えないと思われるが,一方で未経験者にとっては,骨をドリリングすること自体が難しく感じられるだろう.このように,ある症例が簡単か難しいかは,術者の感じ方や経験で大きく異なる.また,このような術者間で感じ方に差がある技術的難易度とは別個に,全身状態や治療部位,患者の個性に基づく難易度が存在する.これらの項目に問題がある場合にインプラント治療の成功率が低下することは,多くの場合質が高いエビデンスで裏づけされている.日本口腔インプラント学会研究推進委員会では,Evidence-Based Difficulty Assessment Toolともいえる,術者の経験によらないインプラント治療の難易度分類を試みている.本稿ではその試案を提示する.(著者抄録)

Language：English   Publishing type：Research paper (scientific journal)  

The clinical remount procedure, which involves remounting the dentures on an articulator with interocclusal records, can effectively reduce occlusal discrepancies. This procedure can be applied not only to new dentures but also to those already in service; however, research in this field is still scarce. This narrative review aims to establish a hypothetical mechanism and possible indications and contraindications for this technique as a basis for further research. Current studies have revealed a high prevalence of malocclusion in delivered dentures. Performing a clinical remount on these existing dentures would enhance the oral function of the denture wearer and would enable effective and accurate correction of the accumulated errors in the jaw relationship in a stable working environment. This technique should be performed if a patient has poor masticatory function or occlusion-related complaints. However, performing a clinical remount on dentures with an excessive anterior-posterior discrepancy between the centric relation and the maximal intercuspal position or on dentures with extremely low occlusal vertical dimension, is considered less effective. The clinical remount procedure remains an essential skill both for fabricating quality dentures and maintaining those already in service.

DOI： 10.3390/healthcare10061067

Web of Science

Scopus

PubMed

researchmap

Language：Japanese   Publisher：(公社)日本口腔インプラント学会  

本邦では口腔外科や補綴,歯周病のいずれかに軸足を置く歯科医師がインプラント治療を担当することが多いように思われる.そのため,特に自身がメインフィールドとする領域以外の治療において正確な難易度を判定する手法があれば,安心安全なインプラント治療を提供することができると考えられる.現在,いくつかのインプラント治療難易度判定ツールが提案されているが,これまでに用いられてきた種々の分類法では難易度をうまく表現できない問題も存在する.たとえば,骨の質,量ともに問題なく咬合にも問題ない下顎臼歯部1歯中間欠損などはイージーケースといっても差し支えないと思われるが,一方で未経験者にとっては,骨をドリリングすること自体が難しく感じられるだろう.このように,ある症例が簡単か難しいかは,術者の感じ方や経験で大きく異なる.また,このような術者間で感じ方に差がある技術的難易度とは別個に,全身状態や治療部位,患者の個性に基づく難易度が存在する.これらの項目に問題がある場合にインプラント治療の成功率が低下することは,多くの場合質が高いエビデンスで裏づけされている.日本口腔インプラント学会研究推進委員会では,Evidence-Based Difficulty Assessment Toolともいえる,術者の経験によらないインプラント治療の難易度分類を試みている.本稿ではその試案を提示する.(著者抄録)

researchmap

Other Link： https://search-tp.jamas.or.jp/index.php?module=Default&action=Link&pub_year=2022&ichushi_jid=J02151&link_issn=&doc_id=20220801250004&doc_link_id=10.11237%2Fjsoi.35.83&url=https%3A%2F%2Fdoi.org%2F10.11237%2Fjsoi.35.83&type=J-STAGE&icon=https%3A%2F%2Fjk04.jamas.or.jp%2Ficon%2F00007_3.gif

Language：English   Publishing type：Research paper (scientific journal)  

Menopausal women are susceptible to visceral obesity, which increases the risk of metabolic disorders. However, the mechanisms of menopause-induced visceral fat accumulation are not fully understood. Circulating levels of receptor activator of nuclear factor-kappa B (NF-κB) ligand (RANKL) are elevated in an animal model of menopause. RANKL, a multifunctional cytokine, activates the NF-κB pathway, which serves as a pivotal mediator of inflammatory responses. Here, we investigated whether RANKL-induced non-canonical NF-κB pathway activation induces inflammation and lipid accumulation in adipose tissues. RANKL induced Tnfa expression via the non-canonical NF-κB pathway in bone marrow cells. We therefore analyzed aly/aly mice, in which the non-canonical NF-κB pathway is not activated, owing to an inactive form of NF-κB-inducing kinase. A postmenopausal obesity model was generated by ovariectomy and subsequent high-fat and high-sucrose diet feeding. In aly/aly mice with postmenopausal obesity, serum RANKL levels were elevated, and hepatic lipid accumulation and adipocyte hypertrophy were suppressed, resulting in reduced macrophage infiltration and inflammatory cytokine mRNA expression in visceral adipose tissue. Furthermore, aly/aly mice showed protection from glucose intolerance and insulin resistance, which were observed in ovariectomized WT obese mice. These findings indicate that non-canonical NF-κB pathway activation via serum RANKL elevation contributes to postmenopausal obesity.

DOI： 10.1530/JOE-21-0424

Web of Science

Scopus

PubMed

researchmap

Language：English   Publisher：Regenerative Biomaterials  

The aim of this study was to investigate titanium membranes (TMs) layered between poly (lactic-co-glycolic acid) (PLGA) containing fluvastatin (FS) for use in guided bone regeneration. Membranes consisting of PLGA, FS-containing PLGA (PLGA-FS), TM layered between PLGA (TM-PLGA) and TM layered between FS-containing PLGA (TM-PLGA-FS) were prepared, and their mechanical and chemical properties were evaluated. The TM groups showed statistically significant differences, in terms of tensile strength and elastic modulus, when compared to the PLGA groups. The release of FS was demonstrated to be higher in the TM-PLGA-FS group than the PLGA-FS group after Day 14. The effect of membrane implantation on the calvaria of Wistar rats was measured using micro-computed tomography (micro-CT) and morphometrical analyses, as well as histological observations. At 4 weeks, the TM-PLGA-FS and TM-PLGA groups were found to have lower bone mineral density but higher bone formation, when compared to the control and PLGA groups. At 8 weeks, the use of TM-PLGA-FS membranes significantly enhanced bone formation in the calvaria model, compared to the other groups. These results suggest that a TM layered between PLGA containing FS potentially enhances bone formation, thus showing good potential as a GBR membrane.

DOI： 10.1093/rb/rbac061

Web of Science

Scopus

PubMed

Language：English   Publishing type：Research paper (scientific journal)  

This case report describes a 70 year-old man with IVA lung cancer who required oral function rehabilitation by fabricating dentures with a simplified clinical remount technique. A pair of dentures were fabricated for a 70-year-old man with stage IVA lung cancer. Due to severe bimaxillary exostoses, the dentures could not properly extend and achieve a peripheral seal. The treatment philosophy was to stabilize the dentures and achieve proper function with optimized occlusion. The simplified Lauritzen clinical remount technique was performed at the time of denture delivery and 3 months later. After the second clinical remount procedure, the patient was able to eat meals with the dentures and maintained in a stable condition. Compared with the original technique, the simplified Lauritzen clinical remount omits the facebow transfer and keeps the condylar guidance setting and the Bennett angle unchanged during the adjustment. The prostheses are mounted to a type 3, non-arcon type articulator with anterior stop screws attached to the bilateral condylar parts. With the aid of anterior stop screws, the eccentric movement of dentures can be differentiated on a millimeter scale and balanced easily. It is effective to use occlusal-optimized dentures and the clinical remount technique, especially in difficult cases.

DOI： 10.3390/healthcare10040682

Web of Science

Scopus

PubMed

researchmap

Language：English   Publishing type：Research paper (scientific journal)  

BACKGROUND: Low bone quantity and quality are serious problems that affect the prognosis of implants in the cosmetic field. Therefore, artificial bone substitutes are frequently used. However, whether there is a difference in the effect of either bone substitute on soft tissue healing is unclear given their greatly different absorbability. In this study, we used hydroxyapatite (HAp) and carbonate apatite (CO3Ap) as bone substitutes to analyze the epithelial and connective tissue healing after tooth extraction. METHODS: In vitro, oral mucosa-derived epithelial cells (OECs) collected from 4-day-old Wistar rats were seeded on HAp or CO3Ap and evaluated for adhesion, proliferation, migration, apoptosis, and morphology. Fibroblasts (FBs) were also analyzed for their ability to express collagen. In vivo, the extraction of maxillary right first (M1) and second molars (M2) of 6-week-old male Wistar rats was performed, followed by insertion of HAp or CO3Ap granules into the M1 and M2 sites. The oral mucosal healing process was then evaluated histochemically after 7 and 14 days. RESULTS: In vitro, high collagen expression by FBs in the CO3Ap group was observed and the surface analysis showed spreading of the FBs on the CO3Ap surface. However, the activity of OECs was suppressed on CO3Ap. Two weeks after CO3Ap implantation, soft tissue healing was observed, and recovery of the connective tissue was observed on the remaining CO3Ap. CONCLUSIONS: Our results suggest that the formation of soft tissues, including connective tissue, was promoted by CO3Ap in the extraction socket within a short period.

DOI： 10.1186/s40729-022-00408-4

Web of Science

PubMed

researchmap

Language：Japanese   Publisher：(公社)日本口腔インプラント学会  

researchmap

Language：Japanese   Publishing type：Research paper (scientific journal)  

インプラント治療後の残存歯および口腔関連QOLを他の歯科補綴治療との比較から明らかにすることを目的に、過去にインプラント義歯(IP)、ブリッジ(BR)、床義歯(RPD)治療前後に口腔関連QOL評価を受けた患者105名(IP群58名、BR群27名、RPD群20名)を対象に、6年経過後の追跡調査時における対象補綴装置の6年累積生存率、残存歯数、および口腔関連QOLを評価した。その結果、6年累積生存率はIP群94.7%、BR群77.4%、RPD群33.3%で、IP群は他の2群に比べ有意に高かった。追跡調査時の残存歯数については、IP群がRPD群に比べ有意に多かった。また、口腔関連QOLについては、IP群では治療開始前後、および治療前・追跡調査時のいずれにおいても有意に高くなっていたのに対し、他の2群ではいずれも有意差は認めなかった。

Language：Japanese   Publisher：(公財)8020推進財団  

インプラント治療後の残存歯および口腔関連QOLを他の歯科補綴治療との比較から明らかにすることを目的に、過去にインプラント義歯(IP)、ブリッジ(BR)、床義歯(RPD)治療前後に口腔関連QOL評価を受けた患者105名(IP群58名、BR群27名、RPD群20名)を対象に、6年経過後の追跡調査時における対象補綴装置の6年累積生存率、残存歯数、および口腔関連QOLを評価した。その結果、6年累積生存率はIP群94.7%、BR群77.4%、RPD群33.3%で、IP群は他の2群に比べ有意に高かった。追跡調査時の残存歯数については、IP群がRPD群に比べ有意に多かった。また、口腔関連QOLについては、IP群では治療開始前後、および治療前・追跡調査時のいずれにおいても有意に高くなっていたのに対し、他の2群ではいずれも有意差は認めなかった。

J-GLOBAL

researchmap

Language：Japanese   Publisher：(公財)8020推進財団  

インプラント治療後の残存歯および口腔関連QOLを他の歯科補綴治療との比較から明らかにすることを目的に、過去にインプラント義歯(IP)、ブリッジ(BR)、床義歯(RPD)治療前後に口腔関連QOL評価を受けた患者105名(IP群58名、BR群27名、RPD群20名)を対象に、6年経過後の追跡調査時における対象補綴装置の6年累積生存率、残存歯数、および口腔関連QOLを評価した。その結果、6年累積生存率はIP群94.7%、BR群77.4%、RPD群33.3%で、IP群は他の2群に比べ有意に高かった。追跡調査時の残存歯数については、IP群がRPD群に比べ有意に多かった。また、口腔関連QOLについては、IP群では治療開始前後、および治療前・追跡調査時のいずれにおいても有意に高くなっていたのに対し、他の2群ではいずれも有意差は認めなかった。

Language：English   Publisher：Nihon University School of Dentistry  

<p><b>Purpose:</b> The purpose of this study was to evaluate the 3-year cumulative survival rates of posterior single monolithic zirconia crowns (MZCs) and their antagonists, and to analyze the influencing factors.</p><p><b>Methods:</b> The clinical outcomes of posterior single MZCs and their abutment teeth with antagonists, and the antagonists between April 2014 and September 2020 were evaluated retrospectively. The 3-year cumulative survival rates were calculated and associations between the survival time and predictor variables (“Jaw”, “Tooth”, and “Pulpal condition”) were also verified using Cox proportional hazards models and hazard ratios (HRs).</p><p><b>Results:</b> The 3-year cumulative survival rate of single MZCs was 89.8% (9 of 177 MZCs, 95% confidence interval (CI): 80.0-95.1%). Cox proportional hazards models showed non-vital teeth were significantly associated with failure (HR: 2.76e + 9, <i>P</i> = 0.012). The 3-year cumulative survival rate of antagonists was 94.8% (7 of 171 antagonists, 95% CI: 89.3-97.6%). Non-vital antagonists were also identified as an independent predictor for failure in Cox proportional hazards models (HR: 7.83, <i>P</i> = 0.03).</p><p><b>Conclusion:</b> Although posterior single MZCs were clinically acceptable, non-vital pulpal condition could be a potential risk factor for failures in the abutment and antagonist teeth of MZCs.</p>

DOI： 10.2334/josnusd.22-0221

Web of Science

Scopus

PubMed

CiNii Research

Language：Japanese   Publisher：Japan Prosthodontic Society  

<p>Japan Prosthodontic Society (JPS) holds scientific meetings and publishes Journal of Prosthodontic Research (JPR), to widely publicize the research activities of its members. Comparing the presentations at these conferences and the papers published in JPR with the presentations at prosthodontic societies in Europe and the United States and the papers published in their journals, there can be seen unique characteristics of the JPS/JPR, such as relatively larger number of biological and material researches. In addition, JPR has the highest impact factor among international journals related to prosthodontics, however, when we evaluate the journal in conjunction with other indicators, we feel that there is room for improvement in terms of the international spread of contributors and the international impact of the articles published.</p>

DOI： 10.2186/ajps.14.137

CiNii Research

Language：Japanese   Publisher：Japan Prosthodontic Society  

<p>While restoring the patients with short-span partial edentulism, it is often difficult to determine the type of final prosthesis. In such cases that can be restored with both fixed and removable partial dentures, meticulous efforts should be made to fabricate prostheses with functionality, aesthetics, cleanability, and durability. The subject of this article is “Proper treatment planning for short-span partial edentulism.” Several cases restored with fixed or removable partial denture solely, or combined, would be presented. The decision-making progress would be discussed as well.</p>

DOI： 10.2186/ajps.14.46

CiNii Research

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1016/j.jmbbm.2021.104771

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.3390/ma14216489

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.3390/ma14195760

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.2334/josnusd.21-0265

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1186/s40729-021-00381-4

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.3390/jcm10153437

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.3390/ma14164457

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.3390/ma14143997

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.3390/healthcare9060660

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.3390/ijerph18136776

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.3390/jcm10112386

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.4012/dmj.2020-190

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.3390/ma14092145

Language：Japanese   Publishing type：Research paper (scientific journal)  

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.3390/medicina56110623

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.3390/jcm9103331

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.2334/josnusd.19-0318

Language：Japanese   Publishing type：Research paper (scientific journal)  

Language：Japanese   Publishing type：Research paper (other academic)  

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1111/joor.12920

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.3390/jcm9082627

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.2334/josnusd.19-0332

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1111/ggi.13911

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.3390/MA13092038

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1038/s41598-020-61724-6

Language：Japanese  

(公社)日本補綴歯科学会は、全国の歯学部学生を対象とした臨床技能コンペティション(課題:保険収載CAD/CAM冠を想定した支台歯形成、下顎右側第二小臼歯)を開催した。26校の代表者各1名が行った支台歯形成を審査し、上位6名の学生を選出した。これらの学生を対象としたオンサイトでの最終実技審査を行い、審査結果の公表および優秀者の表彰を同日に行った。審査は形成模型のデジタルスキャンデータを使用して算出されたテーパーと削除量を参考に、評価者による総括評価とした。事後アンケートでは肯定的な回答が寄せられ、特に参加学生にとって本企画が歯科技能向上に対する研鑽意欲を喚起するものであったことが明らかになった。(著者抄録)

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.3390/app9224908

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1016/j.jpor.2018.10.009

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1021/acsbiomaterials.9b01045

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1021/acsbiomaterials.9b00499

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1016/j.jpor.2018.10.009

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1021/acsbiomaterials.9b01045

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.3390/jcm8101560

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.3390/jcm8101560

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.3390/ma12172748

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1002/jbm.a.36651

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.4012/dmj.2018-274

Language：Japanese  

Language：Japanese  

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1002/jbm.a.36597

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1002/jbm.a.36597

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1089/wound.2018.0834

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1002/jbm.a.36651

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1007/s10266-018-0376-z

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.4012/dmj.2018-274

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1021/acsbiomaterials.9b00499

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.3390/ma12172748

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1007/s10266-018-0376-z

Language：Japanese  

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.4103/0972-4052.244585

Language：English  

A peptide that blocks the interaction of NF-B p65 subunit with Smad4 enhances BMP2-induced osteogenesis
Bone morphogenetic protein (BMP) potentiates bone formation through the Smad signaling pathway in vitro and in vivo. The transcription factor nuclear factor κB (NF-κB) suppresses BMP-induced osteoblast differentiation. Recently, we identified that the transactivation (TA) 2 domain of p65, a main subunit of NF-κB, interacts with the mad homology (MH) 1 domain of Smad4 to inhibit BMP signaling. Therefore, we further attempted to identify the interacting regions of these two molecules at the amino acid level. We identified a region that we term the Smad4-binding domain (SBD), an amino-terminal region of TA2 that associates with the MH1 domain of Smad4. Cell-permeable SBD peptide blocked the association of p65 with Smad4 and enhanced BMP2-induced osteoblast differentiation and mineralization without affecting the phosphorylation of Smad1/5 or the activation of NF-κB signaling. SBD peptide enhanced the binding of the BMP2-inudced phosphorylated Smad1/5 on the promoter region of inhibitor of DNA binding 1 (Id-1) compared with control peptide. Although SBD peptide did not affect BMP2-induced chondrogenesis during ectopic bone formation, the peptide enhanced BMP2-induced ectopic bone formation in subcortical bone. Thus, the SBD peptide is useful for enabling BMP2-induced bone regeneration without inhibiting NF-κB activity.

DOI： 10.1002/jcp.26571

Language：Japanese  

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.3390/ma11071201

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.4012/dmj.2017-334

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.3390/ma11071201

Language：English   Publishing type：Research paper (scientific journal)  

Language：English   Publishing type：Research paper (scientific journal)  

Language：English   Publishing type：Research paper (scientific journal)  

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1186/s40729-018-0121-y

Language：English   Publishing type：Research paper (scientific journal)  

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1186/s40729-017-0112-4

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.4012/dmj.2017-022

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.4012/dmj.2017-334

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1002/jcp.26571

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1002/jor.23562

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1002/jor.23562

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1007/s10266-017-0298-1

Language：Japanese  

Language：English   Publishing type：Research paper (scientific journal)  

A number of finite element analyses (FEAs) for the dental implant were performed without regard for preload and with all interfaces ‘fixed-bonded’. The purpose of this study was comparing the stress distributions between the conventional FEA model with all contacting interfaces ‘fixed-bonded’ (bonded model) and the model with the interfaces of the components in ‘contact’ with friction simulated as a preloaded implant (contact model). We further verified the accuracy of the result of the FEA using model experiment. In the contact model, the stress was more widely distributed than in the bonded model. From the model study, the preload induced by screw tightening generated strain at the peri-implant bone, even before the application of external force. As a result, the bonded model could not reproduce the mechanical phenomena, whereas the contact model is considered to be appropriate for analysing mechanical problems.

DOI： 10.1080/10255842.2016.1236370

Language：English   Publishing type：Research paper (scientific journal)  

Language：English  

DOI： 10.1016/j.jpor.2016.01.004

Language：English   Publishing type：Research paper (scientific journal)  

Language：English   Publishing type：Research paper (scientific journal)  

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1186/s13287-016-0367-3

Language：English   Publishing type：Research paper (scientific journal)  

Tissue Reaction to a Novel Bone Substitute Material Fabricated With Biodegradable Polymer-Calcium Phosphate Nanoparticle Composite.
PURPOSE: The aim of this study was to evaluate the effectiveness of a novel bone substitute material fabricated using a biodegradable polymer-calcium phosphate nanoparticle composite. METHODS: Porous structured poly-L-lactic acid (PLLA) and hydroxyapatite (HA) nanoparticle composite, which was fabricated using solid-liquid phase separation and freeze-drying methods, was grafted into bone defects created in rat calvarium or tibia. Rats were killed 4 weeks after surgery, and histological analyses were performed to evaluate new bone formation. RESULTS: Scanning electron microscopic observation showed the interconnecting pores within the material and the pore diameter was approximately 100 to 300 μm. HA nanoparticles were observed to be embedded into the PLLA beams. In the calvarial implantation model, abundant blood vessels and fibroblastic cells were observed penetrating into pores, and in the tibia model, newly formed bone was present around and within the composite. CONCLUSIONS: The PLLA-HA nanoparticle composite bone substitute developed in this study showed biocompatibility, elasticity, and operability and thus has potential as a novel bone substitute.

DOI： 10.1097/ID.0000000000000447

Language：English   Publishing type：Research paper (scientific journal)  

Therapeutic interactions between mesenchymal stem cells for healing medication-related osteonecrosis of the jaw.
BACKGROUND: Mesenchymal stem cells (MSCs) have been isolated from a variety of tissues, including bone marrow, adipose, and mucosa. MSCs have the capacity for self-renewal and differentiation. Reports have been published on the systemic administration of MSCs leading to functional improvements by engraftment and differentiation, thus providing a new strategy to regenerate damaged tissues. Recently, it has become clear that MSCs possess immunomodulatory properties and can therefore be used to treat diseases. However, the therapeutic effect mechanisms of MSCs are yet to be determined. Here, we investigated these mechanisms using a medication-related osteonecrosis of the jaw (MRONJ)-like mouse model. METHODS: To generate MRONJ-like characteristics, mice received intravenous zoledronate and dexamethasone two times a week. At 1 week after intravenous injection, maxillary first molars were extracted, and at 1 week after tooth extraction, MSCs were isolated from the bone marrow of the mice femurs and tibias. To compare "diseased MSCs" from MRONJ-like mice (d-MSCs) with "control MSCs" from untreated mice (c-MSCs), the isolated MSCs were analyzed by differentiation and colony-forming unit-fibroblast (CFU-F) assays and systemic transplantation of either d-MSCs or c-MSCs into MRONJ-like mice. Furthermore, we observed the exchange of cell contents among d-MSCs and c-MSCs during coculture with all combinations of each MSC type. RESULTS: d-MSCs were inferior to c-MSCs in differentiation and CFU-F assays. Moreover, the d-MSC-treated group did not show earlier healing in MRONJ-like mice. In cocultures with any combination, MSC pairs formed cell-cell contacts and exchanged cell contents. Interestingly, the exchange among c-MSCs and d-MSCs was more frequently observed than other pairs, and d-MSCs were distinguishable from c-MSCs. CONCLUSIONS: The interaction of c-MSCs and d-MSCs, including exchange of cell contents, contributes to the treatment potential of d-MSCs. This cellular behavior might be one therapeutic mechanism used by MSCs for MRONJ.

DOI： 10.1186/s13287-016-0367-3

Language：Japanese  

DOI： 10.4139/sfj.67.297

Language：English   Publishing type：Research paper (scientific journal)  

Soft tissue sealing around dental implants based on histological interpretation.
PURPOSE: The aim of this study was to provide an overview on the biology and soft tissue sealing around dental implants and teeth. STUDY SELECTION: This is a narrative review performed through scientific articles published between 1977 and 2014, indexed in MEDLINE and PubMed databases. The study selected articles that focused on epithelial sealing around dental implant or teeth with cell biology and histology of soft tissue. RESULTS: Implant therapy has been widely applied in dental rehabilitation for many years, with predictable long-term results. The longevity and functionality of dental implants is dependent on both osseointegration around the implant body and the establishment of a soft tissue barrier that protects the underlying hard tissue structures and the implant itself. The health and stability of the peri-implant mucosa also affects the esthetics of the implant. The healing and maintenance of the epithelial and connective tissues around implants are increasingly recognized as being fundamental to implant success. However, there has been little research into the function or formation of the soft tissue seal around dental implants, and the roles of this unique mucosal interface remain unclear. CONCLUSIONS: This narrative review explores the extent of the current knowledge of soft tissue barriers around implants from both a basic and clinical perspective, and aims to consolidate this knowledge and highlight the most pertinent questions relating to this area of research.

DOI： 10.1016/j.jpor.2015.07.001

Language：Japanese   Publishing type：Research paper (scientific journal)  

インプラント治療が補綴歯科臨床の場で必要不可欠となった今日においても、未だ「インプラント支持固定性補綴装置に付与すべき咬合様式」のガイドラインまたは明確な基準は示されていない。本ポジションペーパーにおいては、その推奨されるべき一定の基準を示し、臨床上の指針として提案することを目的とした。本稿では、典型的な欠損形態である下顎大臼歯部欠損にインプラント支持補綴装置を装着した場合を想定している。口腔インプラントを適用した治療に関して経験豊富なエキスパートパネルを、(公社)日本補綴歯科学会会員から選出し、臨床の現場における事象を検証した結果として得られた意見を集積し、パネル会議で検討した。その結果、補綴装置の形態、咬合接触点の位置と数、側方運動時の咬合接触の有無、歯根膜の変位量の考慮、補綴装置咬合面の材料等に関する知見が得られ、推奨される一定の基準が示された。しかしながら、上記内容は明確な根拠に支持されるものではなく、治療効果と術後経過に関する研究が不十分であり、今後さらなる知見の集積と検証のもとに、ガイドラインを策定すべきである。(著者抄録)

DOI： 10.2186/ajps.8.1

Language：English   Publishing type：Research paper (scientific journal)  

Language：English   Publishing type：Research paper (scientific journal)  

Additional Effects of Platelet-Rich Fibrin on Bone Regeneration in Sinus Augmentation With Deproteinized Bovine Bone Mineral: Preliminary Results.
PURPOSE: Platelet-rich fibrin (PRF) has been reported to contribute to bone regeneration; however, little is known about details with deproteinized bovine bone mineral (DBBM). This study aims to evaluate additional effects of PRF on bone regeneration in sinus augmentation with DBBM. MATERIALS AND METHODS: Sinus augmentations were made with DBBM/PRF mixture as lateral approach for 4 sinuses from 4 patients. Bone biopsies were obtained from posterior maxilla at the implant placements 7 or 10 months after sinus augmentations. Histological observations and histomorphometric analyses from augmented areas were performed. RESULTS: The new bone formation was found around the DBBMs with very good contact while surfaces of DBBMs were partly resorbed. Osteoclasts recognized the DBBMs for remodeling, followed by new bone running. The histomorphometric analyses revealed that mean percentages of newly formed bone were 31.7 ± 1.2%, 21.0 ± 1.0%, 38.0 ± 0.6%, and 47.0 ± 0.6%, respectively (mean 34.5 ± 5.7%). CONCLUSIONS: Additional effects of PRF could be found because of higher percentages of newly bone formation by DBBM/PRF mixture than those by DBBM individual in previous reports.

DOI： 10.1097/ID.0000000000000306

Language：English   Publishing type：Research paper (scientific journal)  

Acceleration of hard and soft tissue healing in the oral cavity by a single transmucosal injection of fluvastatin-impregnated poly (lactic-co-glycolic acid) microspheres. An in vitro and rodent in vivo study.
Antihyperlipidemic drug statins reportedly promote both bone formation and soft tissue healing. We examined the effect of sustained-release, fluvastatin-impregnated poly(lactic-co-glycolic acid) (PLGA) microspheres on the promotion of bone and gingival healing at an extraction socket in vivo, and the effect of fluvastatin on epithelial cells and fibroblasts in vitro. The maxillary right first molar was extracted in rats, then one of the following was immediately injected, as a single dose, into the gingivobuccal fold: control (no administration), PLGA microspheres without a statin (active control), or PLGA microspheres containing 20 or 40 μg kg(-1) of fluvastatin. At days 1, 3, 7, 14, and 28 after injection, bone and soft tissue healing were histologically evaluated. Cell proliferation was measured under the effect of fluvastatin at dosages of 0, 0.01, 0.1, 1.0, 10, and 50 μM. Cell migration and morphology were observed at dosages of 0 and 0.1 μM. Following tooth extraction, the statin significantly enhanced bone volume and density, connective tissue volume, and epithelial wound healing. In the in vitro study, it promoted significant proliferation and migration of epithelial cells and fibroblasts. A single dose of topically administered fluvastatin-impregnated PLGA microspheres promoted bone and soft tissue healing at the extraction site.

DOI： 10.1088/1748-6041/11/1/015001

Language：English   Publishing type：Research paper (scientific journal)  

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1111/cid.12205

Language：English   Publishing type：Research paper (scientific journal)  

Primary Stability of a Hybrid Implant Compared with Tapered and Cylindrical Implants in an Ex Vivo Model.
BACKGROUND: Hybrid implants are thought to offer good stability and bone-implant contact. PURPOSE: This study sought to evaluate the primary stability of a hybrid implant compared with tapered and cylindrical implants in an ex vivo model and compare undersized drilling with standard drilling in attaining primary stability. MATERIALS AND METHODS: Hybrid (Straumann® Bone Level; BL), tapered (Straumann® Tapered Effect; TE), and cylindrical (Straumann® Standard Plus; SP) implants were inserted into 15 mm-thick porcine iliac crest blocks using standard (corticocancellous or cancellous bone) or undersized (cancellous bone only) drilling (n = 7). Stability was evaluated using maximum insertion torque (MIT), implant stability quotient (ISQ), and push-out tests. RESULTS: The mean MIT for BL and TE was significantly higher than that for SP (p < 0.05). With standard drilling in corticocancellous bone, the mean ISQ for TE was significantly higher than that for SP (p < .05). The mean MIT, ISQ, and push-out test scores for BL, TE, and SP with standard drilling in corticocancellous bone were significantly higher than those with undersized or standard drilling in cancellous bone (p < .05). CONCLUSIONS: In this ex vivo model, the hybrid implant demonstrated promising primary stability compared with the cylindrical implant. The improved stability of the hybrid implant was especially evident in cancellous bone. The influence of the cortical layer should be recognized when selecting implants for primary stability.

DOI： 10.1111/cid.12205

Language：English   Publishing type：Research paper (scientific journal)  

Language：English   Publishing type：Research paper (scientific journal)  

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1007/s10266-014-0151-8

Language：English   Publishing type：Research paper (scientific journal)  

Effects of CaCl₂ hydrothermal treatment of titanium implant surfaces on early epithelial sealing.
Improvement of oral epithelial adhesion to titanium (Ti) may significantly enhance the efficacy of dental implants. We aimed to investigate whether calcium chloride (CaCl2) hydrothermally treated (HT) Ti could promote sealing of the peri-implant epithelium (PIE) around the implant. Right maxillary first molars were extracted from rats and replaced with either CaCl2-HT implants (Ca-HT group), distilled water-HT implants (DW-HT group), or untreated implants (Cont group). After 4 weeks, the implant-PIE interface of the Ca-HT group exhibited a band of immunoreactive laminin-332, similar to the tooth-junctional epithelium interface, which was absent in the Cont and DW-HT groups at the upper portion. We also investigated the effect of Ca-HT on the attachment of rat oral epithelial cells (OECs). OEC adherence onto Ca-HT Ti plates was stronger with higher expression levels of adhesion proteins compared with Cont and DW-HT groups. These results indicate that HT with CaCl2 improves the integration of soft tissue cells with the Ti implant at 4 weeks after implantation, which might facilitate the development of a soft tissue barrier around the implant.

DOI： 10.1016/j.colsurfb.2015.04.025

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1097/ID.0000000000000306

Language：English   Publishing type：Research paper (scientific journal)  

Language：English   Publishing type：Research paper (scientific journal)  

Language：English   Publishing type：Research paper (scientific journal)  

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1016/j.prosdent.2014.04.007

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1111/cid.12043

Language：English   Publishing type：Research paper (scientific journal)  

Relationship between the CT Value and Cortical Bone Thickness at Implant Recipient Sites and Primary Implant Stability with Comparison of Different Implant Types.
BACKGROUND: Studies have shown that bone quality at the implant recipient site can influence primary stability. PURPOSE: The aims of this study were to explore the quantitative estimation of the primary stability of implants preoperatively using CT values and to examine the effect of different implant designs with recommended socket preparation on primary stability. MATERIALS AND METHODS: Forty-four fresh porcine femoral heads were prepared. The bone surrounding implant sockets was preoperatively evaluated by helical CT. Forty-four implants (φ 4.3 × 10 mm), 22 straight and 22 tapered, were placed according to the manufacturer's instructions. The insertion torque value (ITV), implant stability quotient (ISQ), and removal torque value (RTV) were recorded as indicators of primary implant stability. RESULTS: Significant correlations and linear relationships were found between the CT value and ITV, ISQ, and RTV for both straight and tapered implants (Spearman's correlation coefficient, p < .001; linear regression analysis, p < .01). Tapered implants had a significantly higher ITV than straight implants (analysis of covariance, p < .01). CONCLUSIONS: Obtained results suggest that the primary stability of implants could be quantitatively estimated using the CT value preoperatively, indicating the CT value of bone surrounding an implant can contribute considerably to implant planning and design choice in clinical situations.

DOI： 10.1111/cid.12261

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.11607/jomi.3219

Language：English   Publishing type：Research paper (scientific journal)  

DOI： http://dx.doi.org/10.1016/j.jpor.2013.11.001

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1016/j.jpor.2013.11.001

Language：English   Publishing type：Research paper (scientific journal)  

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1038/labinvest.2013.152

Language：English   Publishing type：Research paper (scientific journal)  

Fibroblast attachment onto novel titanium mesh membranes for guided bone regeneration.
Titanium mesh is used in orthopedic surgery as a barrier membrane, as it offers suitable characteristics, which allow mechanical support during the formation of new bone. An ideal membrane would facilitate cell attachment onto its surface, thereby helping to stabilize the blood clot and integrate the membrane into the tissue. However, currently available titanium mesh has millimeter-level pore sizes, which lead to soft tissue ingrowth through the pores. Therefore, the aim of this study was to investigate the fibroblast attachment and migration on different designs of novel titanium mesh with micrometer pore size for guided bone regeneration treatment. Six types of novel titanium mesh membrane and three groups of commercially available membranes were used in this study. Fibroblasts were isolated from 4-day-old green fluorescence protein rats and seeded onto membrane surfaces. At 24 h, the cells attached to the membrane surfaces were fixed and stained with DAPI. The blue-stained nuclei on membrane surfaces, and both upper and lower sides were counted. It was shown that different membrane materials, structure and design differ considerably in their capacity for cell attachment to the membrane surface. The novel membranes, especially mesh with 12 pores compared with mesh with multi-pores, allowed the fibroblast attachment on the membrane surface, but hindered the fibroblast migration through the pores into the lower side of the membrane, which is associated with the defect area in the clinical condition.

DOI： 10.1007/s10266-014-0151-8

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1371/journal.pone.0090681

Language：English   Publishing type：Research paper (scientific journal)  

Language：English   Publishing type：Research paper (scientific journal)  

Bacterial adhesion affinities of various implant abutment materials
Objectives: To investigate bacterial adhesion to various abutment materials. Material and methods: Thirty volunteers participated in this study. Resin splints were fabricated, and five types of disks were fabricated from pure titanium, gold-platinum alloy, zirconia, alumina, and hydroxyapatite with uniform surface roughness and attached to the buccal surface of each splint. After 4 days of use by the subjects, the plaque accumulated on the disk surfaces was analyzed. The bacterial community structure was evaluated using 16S rRNA gene profiling with terminal restriction fragment length polymorphism analysis. The total bacterial count on each disk was estimated using quantitative polymerase chain reaction. Results: Terminal restriction fragment length polymorphism profiles were more similar between tested materials than between subjects, suggesting that the bacterial community structures on the abutment material were influenced more by the individuals than by the type of material. However, the total number of bacteria attached to a disk was significantly different among five materials (P < 0.001, Brunner-Langer test for longitudinal data). Fewer bacteria were attached to the gold-platinum alloy than to the other materials. Conclusions: Gold-platinum alloy appears to be useful material for abutments when considering the accumulation of plaque. However, alternative properties of the abutment material, such as effects on soft tissue healing, should also be taken into consideration when choosing an abutment material. © 2012 John Wiley & Sons A/S.

DOI： 10.1111/j.1600-0501.2012.02574.x

Language：English   Publishing type：Research paper (scientific journal)  

Language：English   Publishing type：Research paper (scientific journal)  

The role of phosphoinositide 3-kinase in adhesion of oral epithelial cells to titanium.
BACKGROUND: Oral epithelial cells (OECs) adhesion to titanium may improve the success rate of implant restoration. PURPOSE: We investigated the mechanism by which OECs adhere to titanium dental implants. MATERIALS AND METHODS: (1) After culturing rat OECs on titanium plates (Ti) or culture dishes in the presence or absence of a phosphoinositide 3-kinase (PI3K) activator or inhibitors and/or growth factors, and OEC morphology under these conditions were analyzed. (2) Right maxillary first molars were extracted and replaced with experimental implants. The rats were treated with or without growth factors. RESULTS: (1) Cell adherence was lower of OECs on Ti than in those on culture dishes, as were the levels of integrin β4 and the continuity of F-actin structures. After PI3K inhibition, markedly reducing adherence to both substrates. In contrast, PI3K activation with activator or insulin-like growth factor restored the OEC adherence and the expression of adhesion molecules on Ti to the levels seen in OECs cultured on dishes. Cell migration was inhibited by PI3K activation. (2) High expression of integrin β4 was observed in the peri-implant epithelia of PI3K-activated rats. CONCLUSION: These findings suggest that PI3K plays an important role in the adhesion of OECs to Ti.

DOI： 10.1016/j.archoralbio.2013.07.013

Language：English   Publishing type：Research paper (scientific journal)  

The role of phosphoinositide 3-kinase in adhesion of oral epithelial cells to titanium.
BACKGROUND: Oral epithelial cells (OECs) adhesion to titanium may improve the success rate of implant restoration. PURPOSE: We investigated the mechanism by which OECs adhere to titanium dental implants. MATERIALS AND METHODS: (1) After culturing rat OECs on titanium plates (Ti) or culture dishes in the presence or absence of a phosphoinositide 3-kinase (PI3K) activator or inhibitors and/or growth factors, and OEC morphology under these conditions were analyzed. (2) Right maxillary first molars were extracted and replaced with experimental implants. The rats were treated with or without growth factors. RESULTS: (1) Cell adherence was lower of OECs on Ti than in those on culture dishes, as were the levels of integrin β4 and the continuity of F-actin structures. After PI3K inhibition, markedly reducing adherence to both substrates. In contrast, PI3K activation with activator or insulin-like growth factor restored the OEC adherence and the expression of adhesion molecules on Ti to the levels seen in OECs cultured on dishes. Cell migration was inhibited by PI3K activation. (2) High expression of integrin β4 was observed in the peri-implant epithelia of PI3K-activated rats. CONCLUSION: These findings suggest that PI3K plays an important role in the adhesion of OECs to Ti.

DOI： 10.1016/j.archoralbio.2013.07.013

Language：English   Publishing type：Research paper (scientific journal)  

Language：English   Publishing type：Research paper (scientific journal)  

Promotive effect of insulin-like growth factor-1 for epithelial sealing to titanium implants
Improvement of oral epithelial adhesion to titanium (Ti) may significantly enhance the efficacy of dental implants. Here, we investigated whether insulin-like growth factor-1 (IGF-1) improved the sealing of the peri-implant epithelium (PIE) around the implant. Right maxillary first molars were extracted from rats and replaced with experimental implants. After 4 weeks of IGF-1 treatment, the implant-PIE interface exhibited a band of immunoreactive laminin-332 (Ln-5), similar to the tooth-junctional epithelium interface, that was partially absent in the untreated group. Immunoelectron microscopy showed a characteristic Ln-5-positive band including hemidesmosomes at both the apical and upper portions of the implant-PIE interface in the IGF-1-treated group. We also investigated the effects of IGF-1/PI3K inhibitors on the dynamics of rat oral epithelial cells (OECs) grown on Ti plates. In OECs cultured with IGF-1, adhesion protein expression increased, cell adherence to Ti plates was higher, and proliferation was faster, whereas migration and apoptosis were induced in the absence of IGF-1 or in the presence of both IGF-1 and a PI3K inhibitor. These data suggest that PI3K mediates the promotive effects of IGF-1, and that IGF-1 is effective at enhancing epithelial integration around Ti implants.

DOI： 10.1002/jbm.a.34608

Language：English   Publishing type：Research paper (scientific journal)  

Promotive effect of insulin-like growth factor-1 for epithelial sealing to titanium implants
Improvement of oral epithelial adhesion to titanium (Ti) may significantly enhance the efficacy of dental implants. Here, we investigated whether insulin-like growth factor-1 (IGF-1) improved the sealing of the peri-implant epithelium (PIE) around the implant. Right maxillary first molars were extracted from rats and replaced with experimental implants. After 4 weeks of IGF-1 treatment, the implant-PIE interface exhibited a band of immunoreactive laminin-332 (Ln-5), similar to the tooth-junctional epithelium interface, that was partially absent in the untreated group. Immunoelectron microscopy showed a characteristic Ln-5-positive band including hemidesmosomes at both the apical and upper portions of the implant-PIE interface in the IGF-1-treated group. We also investigated the effects of IGF-1/PI3K inhibitors on the dynamics of rat oral epithelial cells (OECs) grown on Ti plates. In OECs cultured with IGF-1, adhesion protein expression increased, cell adherence to Ti plates was higher, and proliferation was faster, whereas migration and apoptosis were induced in the absence of IGF-1 or in the presence of both IGF-1 and a PI3K inhibitor. These data suggest that PI3K mediates the promotive effects of IGF-1, and that IGF-1 is effective at enhancing epithelial integration around Ti implants.

DOI： 10.1002/jbm.a.34608

Language：Others   Publishing type：Research paper (scientific journal)  

Bone regeneration of tibial defects in rats with enzymatic hydrogelation of gelatin derivative and recombinant human platelet-derived growth factor-BB complex.

DOI： 10.11607/jomi.2861

Language：English   Publishing type：Research paper (scientific journal)  

Language：English   Publishing type：Research paper (scientific journal)  

Language：English   Publishing type：Research paper (scientific journal)  

Tunneling nanotube formation is essential for the regulation of osteoclastogenesis.
Osteoclasts are the multinucleated giant cells formed by cell fusion of mononuclear osteoclast precursors. Despite the finding of several membrane proteins involving DC-STAMP as regulatory proteins required for fusion among osteoclast precursors, cellular and molecular events concerning this process are still ambiguous. Here we identified Tunneling Nanotubes (TNTs), long intercellular bridges with small diameters, as the essential cellular structure for intercellular communication among osteoclast precursors in prior to cell fusion. Formation of TNTs was highly associated with osteoclastogenesis and it was accompanied with the significant induction of the M-Sec gene, an essential gene for TNT formation. M-Sec gene expression was significantly upregulated by RANKL-treatment in osteoclast precursor cell line. Blockage of TNT formation by Latrunclin B or by M-Sec siRNA significantly suppressed osteoclastogenesis. We have detected the rapid intercellular transport of not only the membrane phospholipids labeled with DiI but also the DC-STAMP-GFP fusion protein through TNTs formed among osteoclast precursors during osteoclastogenesis. Transportation of such regulatory molecules through TNTs would be essential for the process of the specific cell fusion among osteoclast precursors.

DOI： 10.1002/jcb.24433

Language：English   Publishing type：Research paper (scientific journal)  

Tunneling nanotube formation is essential for the regulation of osteoclastogenesis.
Osteoclasts are the multinucleated giant cells formed by cell fusion of mononuclear osteoclast precursors. Despite the finding of several membrane proteins involving DC-STAMP as regulatory proteins required for fusion among osteoclast precursors, cellular and molecular events concerning this process are still ambiguous. Here we identified Tunneling Nanotubes (TNTs), long intercellular bridges with small diameters, as the essential cellular structure for intercellular communication among osteoclast precursors in prior to cell fusion. Formation of TNTs was highly associated with osteoclastogenesis and it was accompanied with the significant induction of the M-Sec gene, an essential gene for TNT formation. M-Sec gene expression was significantly upregulated by RANKL-treatment in osteoclast precursor cell line. Blockage of TNT formation by Latrunclin B or by M-Sec siRNA significantly suppressed osteoclastogenesis. We have detected the rapid intercellular transport of not only the membrane phospholipids labeled with DiI but also the DC-STAMP-GFP fusion protein through TNTs formed among osteoclast precursors during osteoclastogenesis. Transportation of such regulatory molecules through TNTs would be essential for the process of the specific cell fusion among osteoclast precursors.

DOI： 10.1002/jcb.24433

Language：English   Publishing type：Research paper (scientific journal)  

Language：English   Publishing type：Research paper (scientific journal)  

Language：English   Publishing type：Research paper (scientific journal)  

In Vivo and In Vitro Studies of Epithelial Cell Behavior around Titanium Implants with Machined and Rough Surfaces
BACKGROUND: The surface roughness of a dental implant affects the epithelial wound healing process and may significantly enhance implant prognosis. PURPOSE: We explored the influence of surface roughness on peri-implant epithelium (PIE) sealing and down-growth by comparing machine-surfaced (Ms) and rough-surfaced (Rs) implants. MATERIALS AND METHODS: (1) Maxillary first molars were extracted from rats and replaced with Ms or Rs implants. (2) We also compared changes in the morphology of cultured rat oral epithelial cells (OECs) grown on Ms or Rs titanium (Ti) plates. RESULTS: (1) After 4 weeks, the PIE around Ms and Rs implants showed a similar structure to junctional epithelium (JE). At 16 weeks, Rs implants appeared to form a weak epithelial seal at the tissue-implant interface and exhibited markedly less PIE down-growth than Ms implants but was deeper than that observed in natural teeth. (2) We observed less expression of adhesion proteins in OECs cultured on Rs plates than in cells grown on Ms plates. Additionally, cell adherence, migration, and proliferation on Rs plates were lower, whereas apoptosis was reduced on Ms plates. CONCLUSION: Ms implants are a better choice for integration with an epithelial wound healing process.

DOI： 10.1111/cid.12043

Language：English   Publishing type：Research paper (scientific journal)  

In Vivo and In Vitro Studies of Epithelial Cell Behavior around Titanium Implants with Machined and Rough Surfaces
BACKGROUND: The surface roughness of a dental implant affects the epithelial wound healing process and may significantly enhance implant prognosis. PURPOSE: We explored the influence of surface roughness on peri-implant epithelium (PIE) sealing and down-growth by comparing machine-surfaced (Ms) and rough-surfaced (Rs) implants. MATERIALS AND METHODS: (1) Maxillary first molars were extracted from rats and replaced with Ms or Rs implants. (2) We also compared changes in the morphology of cultured rat oral epithelial cells (OECs) grown on Ms or Rs titanium (Ti) plates. RESULTS: (1) After 4 weeks, the PIE around Ms and Rs implants showed a similar structure to junctional epithelium (JE). At 16 weeks, Rs implants appeared to form a weak epithelial seal at the tissue-implant interface and exhibited markedly less PIE down-growth than Ms implants but was deeper than that observed in natural teeth. (2) We observed less expression of adhesion proteins in OECs cultured on Rs plates than in cells grown on Ms plates. Additionally, cell adherence, migration, and proliferation on Rs plates were lower, whereas apoptosis was reduced on Ms plates. CONCLUSION: Ms implants are a better choice for integration with an epithelial wound healing process.

DOI： 10.1111/cid.12043

Language：English   Publishing type：Research paper (scientific journal)  

Current barrier membranes: titanium mesh and other membranes for guided bone regeneration in dental applications.
Research on guided bone regeneration (GBR) is still ongoing, with evidence mainly from preclinical studies. Various current barrier membranes should fulfill the main design criteria for GBR, such as biocompatibility, occlusivity, spaciousness, clinical manageability and the appropriate integration with the surrounding tissue. These GBR characteristics are required to provide the maximum membrane function and mechanical support to the tissue during bone formation. In this review, various commercially available, resorbable and non-resorbable membranes with different characteristics are discussed and summarized for their usefulness in preclinical studies. Membranes offer promising solutions in animal models; however, an ideal membrane has not been established yet for clinical applications. Every membrane type presents both advantages and disadvantages. Titanium mesh membranes offer superb mechanical properties for GBR treatment and its current efficacy in trials will be a focus in this review. A thorough understanding of the benefits and limitations inherent to various materials in specific clinical applications will be of great value and aid in the selection of an optimal membrane for GBR.

DOI： 10.1016/j.jpor.2012.12.001

Language：English   Publishing type：Research paper (scientific journal)  

Effect of titanium surface calcium and magnesium on adhesive activity of epithelial-like cells and fibroblasts.
In the present study, we investigated the hydrothermal treatment of titanium with divalent cation solutions and its effect in promoting the adhesion of gingival epithelial cells and fibroblasts in vitro. Gingival keratinocyte-like Sa3 cells or fibroblastic NIH3T3 cells were cultured for 1 h on experimental titanium plates hydrothermally-treated with CaCl(2) (Ca) or MgCl(2) (Mg) solution, or distilled water (DW). The number and adhesive strengths of attached cells on the substrata were then analyzed. The number of Sa3 cells adhering to the Ca- and Mg-treated plates was significantly larger than in the DW group, but the strength of this adhesion did not differ significantly between groups. In contrast, NIH3T3 cell adhesion number and strength were increased in both the Ca and Mg groups compared to the DW group. Fluorescent microscopic observation indicated that, in all groups, Sa3 had identical expression levels of integrin β4 and development of actin filaments, whereas NIH3T3 cells in the Ca and Mg groups displayed much stronger punctate cytoplasmic signals for vinculin and more bundle-shaped actin filaments than cells in the DW group. As a result, it was indicated that the hydrothermal treatment of titanium with Ca or Mg solution improved the integration of soft tissue cells with the substrata, which may facilitate the development of a soft tissue barrier around the implant.

DOI： 10.1007/s13758-012-0027-9

Language：English   Publishing type：Research paper (scientific journal)  

Effect of titanium surface calcium and magnesium on adhesive activity of epithelial-like cells and fibroblasts.
In the present study, we investigated the hydrothermal treatment of titanium with divalent cation solutions and its effect in promoting the adhesion of gingival epithelial cells and fibroblasts in vitro. Gingival keratinocyte-like Sa3 cells or fibroblastic NIH3T3 cells were cultured for 1 h on experimental titanium plates hydrothermally-treated with CaCl(2) (Ca) or MgCl(2) (Mg) solution, or distilled water (DW). The number and adhesive strengths of attached cells on the substrata were then analyzed. The number of Sa3 cells adhering to the Ca- and Mg-treated plates was significantly larger than in the DW group, but the strength of this adhesion did not differ significantly between groups. In contrast, NIH3T3 cell adhesion number and strength were increased in both the Ca and Mg groups compared to the DW group. Fluorescent microscopic observation indicated that, in all groups, Sa3 had identical expression levels of integrin β4 and development of actin filaments, whereas NIH3T3 cells in the Ca and Mg groups displayed much stronger punctate cytoplasmic signals for vinculin and more bundle-shaped actin filaments than cells in the DW group. As a result, it was indicated that the hydrothermal treatment of titanium with Ca or Mg solution improved the integration of soft tissue cells with the substrata, which may facilitate the development of a soft tissue barrier around the implant.

DOI： 10.1007/s13758-012-0027-9

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1111/j.1600-0501.2011.02305.x

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1111/j.1600-0501.2011.02305.x

Language：English   Publishing type：Research paper (scientific journal)  

Relationship between the bone density estimated by cone-beam computed tomography and the primary stability of dental implants.
OBJECTIVES: The aims of this study were to objectively assess bone quality with density values obtained by cone-beam computed tomography (CBCT) and to determine the correlations between bone density and primary stability of dental implants. MATERIAL AND METHODS: Eighteen Straumann implants were inserted into 18 fresh femoral heads of swine. The bone densities of implant recipient sites were preoperatively determined by the density value using CBCT. The maximum insertion torque value of each implant was recorded using a digital torque meter. Resonance frequency, which represented a quantitative unit called the implant stability quotient (ISQ), was measured using an Osstell Mentor immediately after the implant placement. Spearman's correlation coefficient was calculated to evaluate the correlations among density values, insertion torques, and ISQs at implant placement. RESULTS: The density values ranged from 98 to 902. The mean density value, insertion torque, and ISQ were 591 ± 226, 13.4 ± 5.2 Ncm, and 67.1 ± 8.1, respectively. Statistically significant correlations were found between the density values and insertion torque (r(s) =0.796, P<0.001), density values and ISQ (r(s) =0.529, P=0.024), and insertion torque and ISQ (r(s) =0.758, P<0.001). CONCLUSIONS: The bone quality evaluated by specific CBCT showed a high correlation with the primary stability of the implants. Hence, preoperative density value estimations by CBCT may allow clinicians to predict implant stability. Whether the density values obtained by the CBCT device used in the present study could be applied to other devices requires further elucidation.

DOI： 10.1111/j.1600-0501.2011.02203.x

Language：English   Publishing type：Research paper (scientific journal)  

The difference of fibroblast behavior on titanium substrata with different surface characteristics.
Connective tissue, one of the main components of peri-implant soft tissue, is key to the formation of the peri-implant mucosal seal and helping to prevent epithelial ingrowth. Rough surfaces (Rs), machined surfaces (Ms) or microgrooved surface (MG) are used in the neck area of commercially available titanium implants. In this paper, we aimed to evaluate the influence of surface topography of titanium substratum on connective tissue fibroblasts to gain a better understanding of this effect. Fibroblasts were cultured on titanium plates with Rs, Ms and MG. Adhesion cell number at day 3 was compared and protein distribution of both F-actin and vinculin was determined to observe cellular structure and adhesion. Cell adhesion strength was compared on each surface. At day 3, the number of fibroblasts attached on each substratum was in the order of MG ≈ Ms > Rs. Fibroblasts strongly expressed vinculin in the peripheral area on Ms and MG, and showed strong F-actin architecture. Decreased expression of vinculin and weaker continuity of F-actin were observed on Rs. Fibroblasts on MG were aligned along the grooves, with a significantly higher cell density, whereas cells on Ms and Rs had no clear orientation. The cell adhesion strength was significantly lower on Rs, and no significant difference was seen between MG and Ms. Both MG and Ms showed greater adhesion cell numbers and adhesion strength of fibroblasts when compared with Rs at day 3. The cell density on MG was greater than those on other substrata.

DOI： 10.1007/s10266-011-0029-y

Language：English   Publishing type：Research paper (scientific journal)  

Relationship between the bone density estimated by cone-beam computed tomography and the primary stability of dental implants.
OBJECTIVES: The aims of this study were to objectively assess bone quality with density values obtained by cone-beam computed tomography (CBCT) and to determine the correlations between bone density and primary stability of dental implants. MATERIAL AND METHODS: Eighteen Straumann implants were inserted into 18 fresh femoral heads of swine. The bone densities of implant recipient sites were preoperatively determined by the density value using CBCT. The maximum insertion torque value of each implant was recorded using a digital torque meter. Resonance frequency, which represented a quantitative unit called the implant stability quotient (ISQ), was measured using an Osstell Mentor immediately after the implant placement. Spearman's correlation coefficient was calculated to evaluate the correlations among density values, insertion torques, and ISQs at implant placement. RESULTS: The density values ranged from 98 to 902. The mean density value, insertion torque, and ISQ were 591 ± 226, 13.4 ± 5.2 Ncm, and 67.1 ± 8.1, respectively. Statistically significant correlations were found between the density values and insertion torque (r(s) =0.796, P<0.001), density values and ISQ (r(s) =0.529, P=0.024), and insertion torque and ISQ (r(s) =0.758, P<0.001). CONCLUSIONS: The bone quality evaluated by specific CBCT showed a high correlation with the primary stability of the implants. Hence, preoperative density value estimations by CBCT may allow clinicians to predict implant stability. Whether the density values obtained by the CBCT device used in the present study could be applied to other devices requires further elucidation.

DOI： 10.1111/j.1600-0501.2011.02203.x

Language：English   Publishing type：Research paper (scientific journal)  

The difference of fibroblast behavior on titanium substrata with different surface characteristics.
Connective tissue, one of the main components of peri-implant soft tissue, is key to the formation of the peri-implant mucosal seal and helping to prevent epithelial ingrowth. Rough surfaces (Rs), machined surfaces (Ms) or microgrooved surface (MG) are used in the neck area of commercially available titanium implants. In this paper, we aimed to evaluate the influence of surface topography of titanium substratum on connective tissue fibroblasts to gain a better understanding of this effect. Fibroblasts were cultured on titanium plates with Rs, Ms and MG. Adhesion cell number at day 3 was compared and protein distribution of both F-actin and vinculin was determined to observe cellular structure and adhesion. Cell adhesion strength was compared on each surface. At day 3, the number of fibroblasts attached on each substratum was in the order of MG ≈ Ms > Rs. Fibroblasts strongly expressed vinculin in the peripheral area on Ms and MG, and showed strong F-actin architecture. Decreased expression of vinculin and weaker continuity of F-actin were observed on Rs. Fibroblasts on MG were aligned along the grooves, with a significantly higher cell density, whereas cells on Ms and Rs had no clear orientation. The cell adhesion strength was significantly lower on Rs, and no significant difference was seen between MG and Ms. Both MG and Ms showed greater adhesion cell numbers and adhesion strength of fibroblasts when compared with Rs at day 3. The cell density on MG was greater than those on other substrata.

DOI： 10.1007/s10266-011-0029-y

Language：English   Publishing type：Research paper (scientific journal)  

Evaluations of epithelial sealing and peri-implant epithelial down-growth around "step-type" implants.
OBJECTIVE: Implant designs that can stimulate and integrate with an epithelial wound-healing process may significantly enhance the efficacy of dental implants. Here, we evaluated the potential of "step-type" implant systems to improve the sealing between the peri-implant epithelium (PIE) and the implant surface, and investigated the effect of implant structure on PIE down-growth. MATERIALS AND METHODS: Right maxillary first molars were extirpated from rats and implanted with either a straight-type or a step-type implant varying in step height and/or width (N(s): 0.8 mm height, 0.1 mm width; W(s): 0.8 mm height, 0.2 mm width; H(s): 0.4 mm height, 0.1 mm width). Maxillae were harvested at various time points over 16 weeks to evaluate laminin-5 distribution as an indicator of wound healing and PIE formation, horse-radish peroxidase (HRP) penetration as a measurement of epithelial sealing, and PIE down-growth formation. RESULTS: In all implant models, the PIE formed from the oral sulcular epithelium and spread apically along the implant surface. In the W(s) group, HRP penetration was detected only in the coronal region of the PIE at 4 weeks, whereas in the straight-type, it was observed in the apical region and the connective tissue. At 16 weeks, the W(s) implants exhibited markedly less PIE down-growth than the Con, N(s) or H(s) implants, and were equivalent to that observed in natural teeth. CONCLUSION: The step-type implant system may have the potential for improving epithelial sealing at the tissue-implant interface, as well as reducing apical PIE down-growth, thus enhancing dental implant efficacy.

DOI： 10.1111/j.1600-0501.2011.02163.x

Language：Japanese  

Language：Japanese  

Language：English   Publishing type：Research paper (scientific journal)  

Local application of fluvastatin improves peri-implant bone quantity and mechanical properties: a rodent study.
Statins are known to stimulate osteoblast activity and bone formation. This study examines whether local application of fluvastatin enhances osteogenesis around titanium implants in vivo. Ten-week-old rats received a vehicle gel (propylene glycol alginate (PGA)) or PGA containing fluvastatin (3, 15, 75 or 300 microg) in their tibiae just before insertion of the implants. For both histological and histomorphometric evaluations undecalcified ground sections were obtained and the bone-implant contact (BIC), peri-implant osteoid volume and mineralized bone volume (MBV) were calculated after 1, 2 and 4 weeks. Using the same models mechanical push-in tests were also performed to evaluate the implant fixation strength. After 1 week the MBV and push-in strength were significantly lower in the 300 microg fluvastatin-treated group than in the other groups (P<0.01). At 2 weeks, however, the BIC and MBV were both significantly higher in the 75 microg fluvastatin-treated group than in the non-fluvastatin-treated groups (P<0.01). Similar tendencies were observed at week 4. Furthermore, the data showed a good correlation between the MBV and the push-in strength. These results demonstrate positive effects of locally applied fluvastatin on the bone around titanium implants and suggest that this improvement in osseointegration may be attributed to calcification of the peri-implant bone.

DOI： 10.1016/j.actbio.2009.10.045

Language：English   Publishing type：Research paper (scientific journal)  

The effect of a single remote injection of statin-impregnated poly (lactic-co-glycolic acid) microspheres on osteogenesis around titanium implants in rat tibia.
The aim of this study was to evaluate the effects of newly developed injectable poly (lactic-co-glycolic acid) (PLGA) microspheres containing fluvastatin on osteogenesis around titanium implants in the rat tibia. After confirmation of the sustained-release profile of fluvastatin from the microspheres by an in vitro assay, the microspheres were administered to the back skin of the rats by a single transdermal injection. At 2 and 4 weeks after the implant surgery, the fluvastatin groups showed enhanced new bone formation around the titanium implants without any influence on the serum biochemistry. In addition, the fluvastatin groups showed increased three-point bending strengths of their femurs. The results of this study indicate that a single remote injection of PLGA/fluvastatin microspheres safely and successfully stimulated bone formation around titanium implants and increased the mechanical properties of bone.

DOI： 10.1016/j.biomaterials.2010.01.016

Language：English   Publishing type：Research paper (scientific journal)  

Simvastatin enhances bone formation around titanium implants in rat tibiae

DOI： 10.1111/j.1365-2842.2009.02011.x

Language：English   Publishing type：Research paper (scientific journal)  

Simvastatin enhances bone formation around titanium implants in rat tibiae

DOI： 10.1111/j.1365-2842.2009.02011.x

Language：Japanese  

Language：Japanese  

Language：English   Publishing type：Research paper (scientific journal)  

The alternation of peri-implant bone response exposed to static lateral load

DOI： 10.1299/jbse.4.326

Language：English   Publishing type：Research paper (scientific journal)  

The alternation of peri-implant bone response exposed to static lateral load
In the present study, peri-implant tissue alteration was studied histologically, and then differences in the gene expression pattern of certain proteins were analyzed using cDNA microarray analysis, under the influence of static lateral load. Beagle dogs were divided into two groups: control and load. Three months after the extraction of mandibular premolars, two implants were placed in each side of the mandible. Each group was further divided into subgroups for loading times of 1, 7 and 14 days. Immediately after the implant installation, a loading device was adhered between the abutments of neighboring implants. Afterward, the expansion screw of the load group was activated by 360-degree rotation, resulting in 0.7 mm horizontal expansion. After sacrificing the dogs, peri-implant tissue was collected, then microarray analysis for the detection of gene expression was performed, as well as histology. Histology indicated that the peri-implant tissue broke down under the influence of the load. Microarray analysis demonstrated the presence of load-sensitive gene expression even after 1 day. Differences in gene expression patterns in concert with static lateral load were demonstrated, even at an early stage post-operation. Further study may help in the diagnosis of pathological overload.

DOI： 10.1299/jbse.4.326

Language：Japanese   Publishing type：Research paper (scientific journal)  

Language：Japanese   Publishing type：Research paper (scientific journal)  

Language：Japanese  

Language：Japanese  

Language：Japanese  

Language：Japanese  

Language：English   Publishing type：Research paper (scientific journal)  

Language：English   Publishing type：Research paper (scientific journal)  

Language：Japanese  

DOI： 10.4139/sfj.49.682

Language：English   Publishing type：Research paper (scientific journal)  

Language：English   Publishing type：Research paper (scientific journal)  

Language：English   Publishing type：Research paper (scientific journal)  

Language：English   Publishing type：Research paper (scientific journal)  

Language：Japanese  

The purpose of this study was to investigate the tissue reaction to three kinds of membranes, Gore-Tex Augmentation Material (GTAM), Vicryl, and Dura Mater, placed on bone defects around the titanium implants. Nine adult dogs weighing 15kg were used in this study. The dogs were sacrificed either 2, 4, or 12 weeks after placement. Vicryl was exposed 2 weeks after placement. Histologically, it was found that Vicryl was completely resorbed, Dura Mater was replaced with connective tissue at 12 weeks, and bone defects using GTAM and Dura Mater but not Vicryl were partially filled with newly-formed bone tissue. Active bone formation was observed in the space between membranes and pre-existing bone tissue.

DOI： 10.2186/jjps.41.188

Language：English   Publishing type：Research paper (scientific journal)  

Language：English   Publishing type：Research paper (scientific journal)  

Language：English   Publishing type：Research paper (scientific journal)  

Language：Japanese  

The influence of diabetes on the implant-bone interface of titanium (Ti) implants, inserted transcortically and extending into the medullary canal of rat tibiae, was examined, and the differences in bone reaction were quantitatively assessed using an image processing system. Thirty male Wister King A rats (aged 5 weeks) were used in this experiment, and they were divided into 2 groups, 15 for the control and 15 for the diabetes-induced (DI) group. They were sacrificed 7, 28 and 84 days after implant placement. Toluidine blue-stained undecalcified sections were prepared for histological observation and image analysis. The Ti implants in the bone marrow area had been almost encapsulated in a thick bone layer. The Ti implants in the DI group had been partially surrounded by a thin bone layer, but the thickness of the surrounding bone had decreased. Quantitative evaluation indicated that the control group showed an increase of the percent of bone contact, the thickness of surrounding bone and the area of surrounding bone throughout the experimental period, while the DI group showed a decrease of the thickness of surrounding bone and the area of surrounding bone except for the percent of bone contact.

DOI： 10.2186/jjps.41.94

Language：English   Publishing type：Research paper (scientific journal)  

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1002/(SICI)1097-4636(199711)37:2<235::AID-JBM13>3.0.CO;2-D

Language：English   Publishing type：Research paper (scientific journal)  

Language：English   Publishing type：Research paper (scientific journal)  

Language：English   Publishing type：Research paper (scientific journal)  

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1016/S0142-9612(96)00051-8

Language：English   Publishing type：Research paper (scientific journal)  

Language：English   Publishing type：Research paper (scientific journal)  

Language：English   Publishing type：Research paper (scientific journal)  

Language：English   Publishing type：Research paper (scientific journal)  

Language：English   Publishing type：Research paper (scientific journal)  

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1002/(SICI)1097-4636(199604)30:4<523::AID-JBM11>3.0.CO;2-I

Language：English   Publishing type：Research paper (scientific journal)  

Language：English   Publishing type：Research paper (scientific journal)  

Presentation type：Symposium, workshop panel (public)  

Venue：熊本   Country：Japan  

Presentation type：Symposium, workshop panel (public)  

Venue：熊本   Country：Japan  

Presentation type：Symposium, workshop panel (public)  

Venue：熊本   Country：Japan  

Language：Japanese   Presentation type：Symposium, workshop panel (public)  

Venue：東京都   Country：Japan  

Language：English   Presentation type：Symposium, workshop panel (public)  

Country：Korea, Republic of  

Language：Japanese   Presentation type：Symposium, workshop panel (public)  

Venue：徳島市   Country：Japan  

Event date： 2009.9

Presentation type：Oral presentation (general)  

Venue：大阪   Country：Japan  

Presentation type：Symposium, workshop panel (public)  

Venue：福岡市   Country：Japan  

Presentation type：Oral presentation (general)  

Venue：北九州市   Country：Japan  

Language：Japanese   Presentation type：Symposium, workshop panel (public)  

Venue：大阪市   Country：Japan  

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：岡山県倉敷市   Country：Japan  

Language：Japanese  

Language：Japanese  

Language：Japanese  

Language：Japanese  

Language：Japanese  

Language：Japanese  

Language：English  

Language：Japanese  

Language：Japanese  

Language：Japanese  

Language：Japanese  

Language：Japanese  

Language：Japanese  

Language：Japanese  

Language：Japanese  

Language：Japanese  

Language：Japanese  

Language：Japanese  

Language：Japanese  

Language：Japanese  

Language：Japanese  

Language：English  

Language：Japanese  

Language：Japanese  

Language：Japanese  

Language：Japanese  

Language：Japanese  

Language：Japanese  

Language：Japanese  

Language：English  

Language：Japanese  

Language：Japanese  

Language：Japanese  

Language：Japanese  

Language：Japanese  

Language：Japanese  

Language：Japanese  

Language：Japanese  

Language：Japanese  

Language：Japanese  

Language：Japanese  

Language：English  

Language：Japanese  

Language：Japanese  

Language：English  

Language：Japanese  

Language：Japanese  

Language：Japanese  

Language：Japanese  

Language：Japanese  

Language：Japanese   Publisher：(株)ヒョーロン・パブリッシャーズ  

Language：Japanese   Publisher：(株)ヒョーロン・パブリッシャーズ  

Language：English   Publisher：(公社)日本補綴歯科学会  

Language：Japanese   Publisher：大阪口腔インプラント研究会  

Language：Japanese   Publisher：(公社)日本口腔インプラント学会  

口腔インプラント治療は欠損補綴において重要な選択肢となっている.インプラント体を口腔内で機能させるためには骨組織との結合性が重要であることは以前から知られているが,その機能を長期間継続させるには軟組織による封鎖性が不可欠であることも忘れてはいけない.この封鎖機構は,天然歯周囲に存在する生物学的幅径と類似した構造を有している.しかし,インプラント周囲における封鎖機能は,天然歯周囲と比較して明らかに低く脆弱である.その原因として,骨に直接埋入される口腔インプラントの周囲では歯根膜が存在せず,解剖学的に血流が不足していることが挙げられる.さらに組織学的にインプラント体と接着する上皮などの封鎖構造が天然歯と比べて明らかに限局的で少なく,上皮細胞のチタンに対する接着性がそもそも低いことなどから,わずかに存在する接着構造も封鎖機能が低いとされている.そのため,このインプラント周囲における軟組織封鎖性を向上させるため,インプラント体の材質,表面性状,さらに周囲組織の細胞活性に関するさまざまな研究が現在も進められているわけである.このような基礎的研究に対する理解は,適応症例の選別やインプラント体や手技の選択,さらには軟組織を考慮したメインテナンスの実践に役立ち,インプラント治療の長期間の成功につながると期待している.(著者抄録)

Language：English   Publisher：(公社)日本口腔インプラント学会  

researchmap

Language：Japanese   Publisher：(株)第一歯科出版  

Language：Japanese   Publisher：(公社)日本補綴歯科学会  

公益社団法人日本補綴歯科学会では,教育問題検討委員会を中心として一般社団法人日本歯科専門医機構が求める,補綴歯科専門医を育成するためにふさわしい教育カリキュラムの構築を進めてきたが,このたびカリキュラムが概ね完成したことから,第131回学術大会でそのあらましを報告した.セミナーでは時間の制約もあり,お伝えできなかった項目もあることから,本稿は改めて会員の先生方に教育カリキュラムの内容を知っていただく目的で執筆した.補綴歯科専門医制度のカリキュラム設計は,ファンダメンタルとして補綴歯科専門医の基本知識・技能を学修する基本研修があり,そのうえに大きく分けて症例を基準とした「ケースシリーズベース評価」と,補綴歯科専門医が満たすべき能力の視点からの「コンピテンスベース評価」の二本立ての評価を行う.これらの評価や学習到達度については,いわば実習帳ともいうべき「補綴歯科専門医研修評価記録」に整理する.本稿では主に補綴歯科専門医研修評価記録の記載方法について概説しつつ,機構認定の補綴歯科専門医資格取得に必要な単位,試験について解説する.現在このカリキュラムは各研修機関で試用を行っており,内容の矛盾点や運用上の問題点の改善のために随時改訂を行っていく予定であるが,基本骨格については概ね固まっていると考えていただいて差し支えない.若手補綴歯科医の指導や,教育を受ける若手歯科医師の参考にしていただきたい.(著者抄録)

Publishing type：Book review, literature introduction, etc.  

Purpose: The aim of this study was to review the literature on current surface modification strategies used to improve the binding efficiency of an emerging biological material, polyetheretherketone (PEEK), with bone and soft tissues. Study selection: This review was based on articles retrieved from PubMed, Google Scholar, Web of Science, and Scien-ceDirect databases. The main keywords used during the search were “polyetheretherketone (PEEK),” “implant,” “surface modification,” “biomaterials,” “bone,” “osseointegration,” and “soft tissue.” Results: The suitability of PEEK surface modification strategies has been critically analyzed and summarized here. Many cell and in vivo experiments in small animals have shown that the use of advanced modification technologies with appropriate surface modification strategies can effectively improve the surface inertness of PEEK, thereby improving its binding efficiency with bone and soft tissues. Conclusions: Surface modifications of PEEK have revealed new possibilities for implant treatment; however, most results are based on in vitro or short-term in vivo evaluations in small animals. To achieve a broad application of PEEK in the field of oral implantology, more in vivo experiments and long-term clinical evaluations are needed to investigate the effects of various surface modifications on the tissue integration ability of PEEK to develop an ideal implant material.

DOI： 10.2186/jpr.JPR_D_22_00138

Scopus

PubMed

researchmap

Language：English   Publishing type：Article, review, commentary, editorial, etc. (scientific journal)  

DOI： 10.3390/healthcare10061067.

Language：Japanese   Publisher：(公社)日本口腔インプラント学会  

本邦では口腔外科や補綴,歯周病のいずれかに軸足を置く歯科医師がインプラント治療を担当することが多いように思われる.そのため,特に自身がメインフィールドとする領域以外の治療において正確な難易度を判定する手法があれば,安心安全なインプラント治療を提供することができると考えられる.現在,いくつかのインプラント治療難易度判定ツールが提案されているが,これまでに用いられてきた種々の分類法では難易度をうまく表現できない問題も存在する.たとえば,骨の質,量ともに問題なく咬合にも問題ない下顎臼歯部1歯中間欠損などはイージーケースといっても差し支えないと思われるが,一方で未経験者にとっては,骨をドリリングすること自体が難しく感じられるだろう.このように,ある症例が簡単か難しいかは,術者の感じ方や経験で大きく異なる.また,このような術者間で感じ方に差がある技術的難易度とは別個に,全身状態や治療部位,患者の個性に基づく難易度が存在する.これらの項目に問題がある場合にインプラント治療の成功率が低下することは,多くの場合質が高いエビデンスで裏づけされている.日本口腔インプラント学会研究推進委員会では,Evidence-Based Difficulty Assessment Toolともいえる,術者の経験によらないインプラント治療の難易度分類を試みている.本稿ではその試案を提示する.(著者抄録)

Language：Japanese   Publisher：(株)デンタルダイヤモンド社  

Language：Japanese   Publisher：(公社)日本補綴歯科学会  

比較的少数歯の中間欠損症例で,ブリッジあるいはパーシャルデンチャーのどちらでも欠損補綴が可能であるような場合,最終補綴装置の選択に迷うことも少なくない.機能性,審美性,清掃性を備え,かつ長期的な使用に耐えうる補綴装置を製作するためには,比較的シンプルな症例であっても様々なことを考慮して選択・設計を行う必要がある.本稿では「少数歯の中間欠損症例における補綴装置の選択」をテーマとし,ブリッジまたはパーシャルデンチャー,あるいはその両方により対処した症例を供覧して補綴装置の選択理由や選択時の考慮事項について述べる.(著者抄録)

Language：English   Publishing type：Article, review, commentary, editorial, etc. (scientific journal)  

DOI： https://doi.org/10.1016/j.jpor.2016.01.004

Language：Japanese   Publishing type：Article, review, commentary, editorial, etc. (scientific journal)  

Language：English   Publishing type：Article, review, commentary, editorial, etc. (scientific journal)  

Language：English   Publishing type：Article, review, commentary, editorial, etc. (scientific journal)  

Type：Competition, symposium, etc. 

Type：Academic society, research group, etc. 

Type：Competition, symposium, etc. 

Type：Competition, symposium, etc. 

Number of participants：2,000

Type：Competition, symposium, etc. 

Number of participants：2,000

Type：Competition, symposium, etc. 

Number of participants：4,000

Type：Competition, symposium, etc. 

Number of participants：4,000

Type：Competition, symposium, etc. 

Type：Competition, symposium, etc. 

Type：Competition, symposium, etc.