Updated on 2024/10/01

Information

 

写真a

 
ITOYAMA TOMOHIRO
 
Organization
Faculty of Dental Science Department of Dental Science Assistant Professor
Title
Assistant Professor
Contact information
メールアドレス
Tel
0926426432
Profile
【研究活動】 ・シュワン細胞由来因子を用いた新規歯周治療の開発 ・歯髄象牙質複合体の老化メカニズムの解析 【臨床活動】 ・保存治療(歯内療法、修復治療) ・外科的歯内療法(歯根端切除術、意図的再植術など) 【教育活動】 ・学部学生に対する臨床実習指導 ・歯科医師の卒後研修指導 ・博士課程大学院生に対する臨床および研究指導
External link

Degree

  • Ph.D.

Research Interests・Research Keywords

  • Research theme:Elucidation of the mechanism of dentin formation through the downstream signaling pathway of dopamine."

    Keyword:dopamine, calcineurin, odontoblast

    Research period: 2022.4 - 2024.3

  • Research theme:To elucidate the mechanism of aging alteration of dentin/pulp complex targeting oxidative stress

    Keyword:oxidative stress, cellular senescence, dentin-pulp complex, superoxide dismutase2

    Research period: 2022.4 - 2024.3

  • Research theme:Analysis of aging mechanism of dentin-pulp complex

    Keyword:senescence, pulp, dentin

    Research period: 2020.4 - 2022.3

  • Research theme:Development of novel regenerative therapy of periodontal tissue using schwann cells derived factor.

    Keyword:periodontal ligament, schwann cells

    Research period: 2020.4 - 2022.3

Awards

  • ワカイ賞

    2024.7   日本歯内療法学会   Superoxide Dismutase 2の発現抑制による歯髄細胞の細胞老化が石灰化誘導能に及ぼす影響

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    Award type:Award from Japanese society, conference, symposium, etc. 

Papers

  • 4-META/MMA-TBB resin containing nano hydroxyapatite induces the healing of periodontal tissue repair in perforations at the pulp chamber floor

    Sugii, H; Yoshida, S; Albougha, MS; Hamano, S; Hasegawa, D; Itoyama, T; Obata, J; Kaneko, H; Minowa, F; Tomokiyo, A; Maeda, H

    CELL BIOCHEMISTRY AND FUNCTION   42 ( 4 )   e4058   2024.6   ISSN:0263-6484 eISSN:1099-0844

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    Language:English   Publisher:Cell Biochemistry and Function  

    We aimed to evaluate the materials based on 4-methacryloxyethyl trimellitate anhydride/methyl methacrylate tri-n-butylborane (Super-bond [SB]) and nano hydroxyapatite (naHAp) for the repair of perforation at pulp chamber floor (PPF) in vitro and in vivo models. SB and naHAp were mixed in the mass ratio of 10% or 30% to produce naHAp/SB. Human periodontal ligament stem cells (HPDLSCs) were cultured on resin discs of SB or naHAp/SB to analyze the effects of naHAp/SB on cell adhesion, proliferation, and cementoblastic differentiation. A rat PPF model was treated with SB or naHAp/SB to examine the effects of naHAp/SB on the healing of defected cementum and periodontal ligament (PDL) at the site of PPF. HPDLSCs were spindle-shaped and adhered to all resin discs. Changing the resin from SB to naHAp/SB did not significantly alter cell proliferation. Both 10% and 30% naHAp/SB were more effective than SB in promoting cementoblastic differentiation of HPDLSCs. In the rat PPF model, 30% naHAp/SB was more effective than SB in promoting the formation Sharpey's fiber-like structures with expression of the PDL-related marker and cementum-like structures with expression of cementum-related markers. In conclusion, 30% naHAp/SB can be the new restorative material for PPF because it exhibited the abilities of adhering to dentin and healing of defected periodontal tissue.

    DOI: 10.1002/cbf.4058

    Web of Science

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  • Effect of Fibrillin-2 on Differentiation into Periodontal Ligament Stem Cell-Like Cells Derived from Human-Induced Pluripotent Stem Cells

    Hamano, S; Yamashita, D; Hasegawa, D; Sugii, H; Itoyama, T; Maeda, H

    STEM CELLS AND DEVELOPMENT   33 ( 9-10 )   228 - 238   2024.5   ISSN:1547-3287 eISSN:1557-8534

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    Language:English   Publisher:Stem Cells and Development  

    Periodontal tissue regeneration is important for preserving teeth. Periodontal ligament stem cells (PDLSCs) are useful in periodontal tissue regeneration; however, tooth extraction is required to obtain these cells. Therefore, we focused on induced pluripotent stem (iPS) cells and established a method to obtain PDLSC-like cells from iPS cells. Specifically, we first differentiated iPS cells into neural crest-like cells (iNCs). Next, we obtained PDLSC-like cells (iPDLSCs) by culturing iNCs on extracellular matrix (ECM) derived from human primary periodontal ligament cells (HPDLCs). This differentiation method suggested that ECM derived from HPDLCs is important for iPDLSC differentiation. Thus, we aimed to identify the PDLSC-inducing factor present in HPDLC-derived ECM in this study. We first performed comprehensive analyses of HPDLC genes and identified fibrillin-2 (FBN2), an ECM-related factor. Furthermore, to clarify the effect of FBN2 on iPDLSC differentiation, we cultured iNCs using ECM derived from HPDLCs with FBN2 knocked down. As a result, expression of PDL-related markers was reduced in iNCs cultured on ECM derived from HPDLCs transfected with FBN2 siRNA (iNC-siFBN2) compared with iPDLSCs. Furthermore, the expression of CD105 (a mesenchymal stem cell marker), proliferation ability, and multipotency of iNC-siFBN2 were lower compared with iPDLSCs. Next, we cultured iNCs on FBN2 recombinant protein; however, expression of PDL-related markers did not increase compared with iPDLSC. The present results suggest the critical involvement of FBN2 in inducing iPDLSCs from iNCs when in fact it does not promote iPDLSC differantiation. Therefore, we need to elucidate the entire HPDLC-ECMs, responsible for iPDLSCs induction.

    DOI: 10.1089/scd.2024.0013

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  • Dopamine is involved in reparative dentin formation through odontoblastic differentiation of dental pulp stem cells

    Fujino, S; Hamano, S; Tomokiyo, A; Sugiura, R; Yamashita, D; Hasegawa, D; Sugii, H; Fujii, S; Itoyama, T; Miyaji, H; Maeda, H

    SCIENTIFIC REPORTS   13 ( 1 )   5668   2023.4   ISSN:2045-2322

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    Language:English   Publisher:Scientific Reports  

    Conventional direct pulp-capping materials induce pulp cells to secrete various biomolecules in pulp tissues that promote reparative dentin formation through induction of odontoblastic differentiation of dental pulp stem cells (DPSCs). However, these biomolecules sometimes induce bone-like dentin with poor sealing properties. Therefore, exploration of biomolecules that allow tight sealing by tubular reparative dentin is required. We recently reported that dopamine (DA) is involved in dentinogenesis. Hence, we investigated the effect of DA on odontoblastic differentiation of DPSCs and reparative dentin formation. Both tyrosine hydroxylase (TH), a DA synthetase, and DA were expressed in odontoblast-like cells in vivo. In vitro, their expression was increased during odontoblastic differentiation of DPSCs. Furthermore, TH-overexpressing DPSCs had promoted odontoblastic differentiation and DA production. Moreover, DA stimulation promoted their differentiation and induced tubular reparative dentin. These results suggest that DA produced by TH is involved in odontoblastic differentiation of DPSCs and has an inductive capacity for reparative dentin formation similar to primary dentin. This study may lead to the development of therapy to preserve vital pulp tissues.

    DOI: 10.1038/s41598-023-32126-1

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  • Dopamine is involved in reparative dentin formation through odontoblastic differentiation of dental pulp stem cells. Reviewed International journal

    @Fujino S, @Hamano S, @Tomokiyo A, #Sugiura R, #Yamashita D, @Hasegawa D, @Sugii H, @Fujii S, @Itoyama T, Miyaji H, @Maeda H.

    2023.4

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1038/s41598-023-32126-1.

  • Decorin Promotes Osteoblastic Differentiation of Human Periodontal Ligament Stem Cells

    Adachi, O; Sugii, H; Itoyama, T; Fujino, S; Kaneko, H; Tomokiyo, A; Hamano, S; Hasegawa, D; Obata, J; Yoshida, S; Kadowaki, M; Sugiura, R; Albougha, MS; Maeda, H

    MOLECULES   27 ( 23 )   2022.12   eISSN:1420-3049

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    Language:English   Publisher:Molecules  

    The aim of this study is to clarify the biological functions of decorin (DCN) in the healing and regeneration of wounded periodontal tissue. We investigated the expression pattern of DCN during the healing of wounded periodontal tissue in rats by immunohistochemistry and the effects of DCN on the osteoblastic differentiation of human periodontal ligament (PDL) stem cells (HPDLSCs) and preosteoblasts by Alizarin red S staining, quantitative reverse transcription-polymerase chain reactions, and western blotting. The expression of DCN was increased around the wounded PDL tissue on day 5 after surgery compared with the nonwounded PDL tissue, whereas its expression was not changed in the osteoblastic layer around the wounded alveolar bone. Furthermore, DCN promoted the osteoblastic differentiation of HPDLSCs, but it did not affect the osteoblastic differentiation of preosteoblasts. ERK1/2 phosphorylation was upregulated during the DCN-induced osteoblastic differentiation of HPDLSCs. DCN did not affect proliferation, migration, or the PDL-related gene expression of HPDLSCs. In conclusion, this study demonstrates that DCN has a role in the healing of wounded periodontal tissue. Furthermore, DCN secreted from PDL cells may contribute to bone healing by upregulating osteoblastic differentiation through ERK1/2 signaling in HPDLSCs, indicating a therapeutic effect of DCN in periodontal tissue regeneration.

    DOI: 10.3390/molecules27238224

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  • Decorin promotes osteoblastic differentiation of human periodontal ligament stem cells. Reviewed International journal

    #Adachi O, @Sugii H, @Itoyama T, @Fujino S, @Kaneko H, @Tomokiyo A, @Hamano S, @Hasegawa D, @Obata J, Yoshida S, #Kadowaki M, #Sugiura R, #Albougha S, @Maeda M.

    Molecules.   2022.11

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.3390/molecules27238224.

  • Involvement of M1/M2 macrophage polarization in reparative dentin formation. Reviewed International journal

    #Kadowaki M, Yoshida S, @Itoyama T, @Tomokiyo A, @Hamano S, @Hasegawa D, @Sugii H, @Kaneko H, #Sugiura R, @Maeda H.

    Life (Basel)   2022.11

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: doi: 10.3390/life12111812

  • Involvement of M1/M2 Macrophage Polarization in Reparative Dentin Formation

    Kadowaki, M; Yoshida, S; Itoyama, T; Tomokiyo, A; Hamano, S; Hasegawa, D; Sugii, H; Kaneko, H; Sugiura, R; Maeda, H

    LIFE-BASEL   12 ( 11 )   2022.11   ISSN:2075-1729 eISSN:2075-1729

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    Language:English   Publisher:Life  

    In cases in which dental pulp tissue is accidentally exposed, direct pulp capping is often performed to induce reparative dentin formation. Although macrophages are essential for the inflammatory response and tissue repair, the emergence pattern and the role of macrophages in dental pulp tissue have not been clarified. Here, we investigated the emergence of M1/M2 macrophages in dental pulp tissue after a direct pulp capping and the effects of M2 macrophages on odontoblastic differentiation of the dental pulp stem cell (DPSC) clones. The emergence of macrophages in dental pulp tissue was investigated using a rat direct pulp capping model. Alizarin Red S staining and quantitative RT-PCR was performed to examine the effect of M2 macrophages on the mineralization and odontoblastic differentiation of DPSC clones. Immunohistochemical staining revealed that M1 macrophages were detected in dental pulp tissue after treatment and increased in number at three days after treatment. However, M2 macrophages gradually increased in number in dental pulp tissue after treatment, with the highest level recorded at seven days post-operation. Additionally, conditioned medium from M2 macrophages induced odontoblast-like differentiation of DPSC clones. These results suggest that macrophages play a role in the inflammatory response and reparative dentin formation after dental pulp exposure.

    DOI: 10.3390/life12111812

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  • Inhibition of c-Jun N-terminal kinase signaling promotes osteoblastic differentiation of periodontal ligament stem cells and induces regeneration of periodontal tissues

    Kaneko, H; Hasegawa, D; Itoyama, T; Yoshida, S; Tomokiyo, A; Hamano, S; Sugii, H; Maeda, H

    ARCHIVES OF ORAL BIOLOGY   134   105323   2022.2   ISSN:0003-9969 eISSN:1879-1506

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    Language:English   Publisher:Archives of Oral Biology  

    Objectives: Few clinical treatments to regenerate periodontal tissue lost due to severe endodontic and periodontal disease have yet been developed. Therefore, the development of new treatment methods for the regeneration of periodontal tissue is expected. The purpose of this study was to investigate the effects of a c-Jun N-terminal kinase (JNK) inhibitor, SP600125, on the osteoblastic differentiation of periodontal ligament stem cells (PDLSCs) in vitro, and the function of SP600125 on the regeneration of alveolar bone in vivo. Design: Alizarin red S staining, quantitative RT-PCR, and western blotting analysis was performed to determine whether SP600125 affects osteoblastic differentiation of human PDLSCs (HPDLSCs) and bone-related intracellular signaling. The effect of SP600125 on the regeneration of alveolar bone was assessed by using a rat periodontal defect model. The healing of periodontal defects was evaluated using micro-CT scans and histological analysis. Results: SP600125 promoted the osteoblastic differentiation such as Alizarin red S-positive mineralized nodule formation and the expression of osteoblast-related genes in HPDLSCs under osteogenic conditions. In addition, this inhibitor upregulated the BMP2 expression and the phosphorylation of Smad1/5/8 in HPDLSCs under the same conditions. The inhibition of Smad1/5/8 signaling by LDN193189 suppressed the SP600125-induced osteoblastic differentiation of HPDLSCs. Furthermore, the application of SP600125 promoted the regeneration of not only alveolar bone but also PDL tissue in periodontal defects. Conclusion: This study suggested that inhibition of JNK signaling promotes the osteoblastic differentiation of HPDLSCs through BMP2-Smad1/5/8 signaling, leading to the regeneration of periodontal tissues such as alveolar bone and PDL tissue.

    DOI: 10.1016/j.archoralbio.2021.105323

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  • Development of a novel direct pulp-capping material using 4-META/MA-TBB resin with nanohydroxyapatite. Invited Reviewed International journal

    @Yoshida S, @Sugii H, @Itoyama T, @Kadowaki M, @Hasegawa D, @Tomokiyo A, @Hamano S, #Ipposhi K, #Yamahita K, @Maeda H.

    Mater Sci Eng C Mater Biol Appl.   2021.11

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    Language:English   Publishing type:Research paper (scientific journal)  

  • Mineral trioxide aggregate immersed in sodium hypochlorite reduce the osteoblastic differentiation of human periodontal ligament stem cells. Invited Reviewed International journal

    #Kozue Yamashita, @Atsushi Tomokiyo, @Taiga Ono, #Keita Ipposhi, #M. Anas Alhasan, @Akira Tsuchiya, @Sayuri Hamano, @Hideki Sugii, @Shinichiro Yoshida, @Tomohiro Itoyama, @Hidefumi Maeda.

    Scientific Reports.   2021.11

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  • Inhibition of c-Jun N-terminal kinase signaling promotes osteoblastic differentiation of periodontal ligament stem cells and induces regeneration of periodontal tissues. Invited Reviewed International journal

    #Hiroshi Kaneko, @Daigaku Hasegawa, @Tomohiro Itoyama, @Shinichiro Yoshida, @Atsushi Tomokiyo, @Sayuri Hamano, @Hideki Sugii, @Hidefumi Maeda.

    Archives of Oral Biology.   2021.11

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  • Secreted Frizzled-Related Protein 1 Promotes Odontoblastic Differentiation and Reparative Dentin Formation in Dental Pulp Cells. Invited Reviewed International journal

    #Ipposhi K, @Tomokiyo A, @Ono T, #Yamashita K, #Anas MA, @Hasegawa D, @Hamano S, @Yoshida S, @Sugii H, @Itoyama T, @Ogawa M, @Maeda H.

    Cells   2021.9

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    Language:English   Publishing type:Research paper (scientific journal)  

  • Iodine potassium iodide が White Mineral Trioxide Aggregate の表面性状および機械的強度に及ぼす影響について. Invited Reviewed International journal

    #山下梢,@友清淳,@小野太雅,#一法師啓太,@濱野さゆり,@杉井英樹,@吉田晋一郎,@糸山知宏,@前田英史

    日歯内療誌   2021.6

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    Language:Japanese   Publishing type:Research paper (scientific journal)  

  • Possible function of GDNF and Schwann cells in wound healing of periodontal tissue Reviewed International journal

    2020.12

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  • 象牙芽細胞分化におけるドーパミンの発現 Invited Reviewed

    藤野翔香、濱野さゆり、糸山知宏、前田英史

    2020.1

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    Language:Japanese   Publishing type:Research paper (scientific journal)  

  • Expression and function of dopamine in odontoblasts Reviewed International journal

    Fujino S,Hamano S,Tomokiyo A, Itoyama T, Hasegawa D, Sugii H, Yoshida S, Washiro A, Nozu A, Ono T, Wada N, Kitamura C, Maeda H.

    2019.10

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Presentations

  • Superoxide Dismutase 2の発現抑制が歯髄細胞の老化に及ぼす影響.

    @糸山知宏, @前田英史

    第46回 日本基礎老化学会  2023.6 

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    Event date: 2024.5

    Language:Japanese  

    Country:Japan  

  • The 4-META/MMA-TBB resin containing nano hydroxyapatite regenerates wounded periodontal tissue at the perforation site. International conference

    @Hideki Sugii, @Shinichiro Yoshida, @Sayuri Hamano, @Daigaku Hasegawa, @Junko Obata, @Tomohiro Itoyama, #Mhd Safwan Albougha, #Fumiko Minowa, @Atsushi Tomokiyo, @Hidefumi Maeda,

    Asian Pacific Endodontic Confederation  2023.8 

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    Event date: 2023.8

    Language:English  

    Country:Japan  

  • AhRシグナルの活性化はヒト歯根膜細胞のMMP12発現を亢進する.

    @門脇正敬,@友清淳,@糸山知宏,@長谷川大学,@濱野さゆり,@杉井英樹,@前田英史

    第158回 日本歯科保存大会春季学術大会  2023.6 

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    Event date: 2023.6

    Language:Japanese  

    Country:Japan  

  • Fibrillin-2がiPS細胞の歯根膜幹細胞様細胞への分化誘導に及ぼす影響について.

    @濱野さゆり、#杉浦梨紗、#山下大輝、@友清淳、@杉井英樹、@吉田晋一郎、@糸山知宏、@前田英史,

    日本再生医療学会  2022.3 

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    Event date: 2023.6

    Language:Japanese  

    Country:Japan  

  • iPS細胞由来歯科膜感細胞様細胞の分化誘導におけるFibrillin-2の役割

    @濱野さゆり, #山下大輝, @糸山知宏, @友清淳, @長谷川大学, @杉井英樹, @兼子大志, #Bara Mardini, @前田英史

    第158回 日本歯科保存大会春季学術大会  2023.6 

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    Event date: 2023.6

    Language:Japanese  

    Country:Japan  

  • 歯内治療医からみた難治性歯痛

    @糸山知宏

    第52回日本慢性疼痛学会  2023.3 

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    Event date: 2023.3

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Country:Japan  

  • ドーパミンは修復象牙質形成能を有する.

    @藤野翔香、#杉浦梨沙、@濱野さゆり、@友清淳、#山下大樹、@長谷川大学、@杉井英樹、@糸山知宏、@前田英史.

    第3回象牙質歯髄治療学会学術大会  2022.9 

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    Event date: 2022.9

    Language:Japanese  

    Country:Japan  

  • Decorinによるヒト歯根膜幹細胞の骨芽細胞様分化誘導に関する分子機構の解析.

    #足立織利恵、@杉井英樹、@糸山知宏、@兼子大志、@友清淳、@濱野さゆり、@長谷川大学、@小幡純子、吉田晋一郎、@藤野翔香、#Mhd Safwan Albougha、@前田英史.

    第156回日本歯科保存学会春季学術大会  2022.6 

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    Event date: 2022.6

    Language:Japanese  

    Country:Japan  

  • Activin A mediates the ALK1-Smad1/5/9 pathway during osteoblastic differentiation of human pre-osteoblasts.

    @HIdeki Sugii, #Mhd Safwan Albougha, #Orie Adachi, #Hiroka Tomita, @Atsushi Tomokiyo, @Sayuri Hamano, @Daigaku Hasegawa, @Shinichiro Yoshida, @Junko Obata, @Tomohiro Itoyama, @Taiga Ono, @Keita ippoushi, @HIdefumi Maeda

    2021.6 

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    Event date: 2022.6

    Language:English  

    Country:Japan  

  • Exosomes derived from human periodontal ligament stem cells promote osteoblastic differentiation of pre-osteoblasts.

    #Mhd Safwan Albougha, @Hideki Sugii, @Atsushi Tomokiyo, @Sayuri Hamano, @Daigaku Hasegawa, Shinichiro Yoshida, @Tomohiro Itoyama, @Shoko Fujino, Taiga Ono, #Orie Adachi, @Hidefumi Maeda.

    2022.6 

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    Event date: 2022.6

    Language:English  

    Country:Japan  

  • Superoxide dismutase 2 is involved in the senescence of dentin-pulp complex

    @Tomohiro Itoyama, @Kenji Watanabe, #Masataka Kadowaki, #Hiroshi Kaneko, @Shinichiro Yoshida, @Takahiko Shimizu, @Hidefumi Maeda

    2021.10 

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    Event date: 2021.10 - 2021.11

    Language:English  

    Country:Japan  

  • The effect of JNK inhibition on the osteoblastic differentiation of periodontal ligament stem cells and the regeneration of periodontal tissue in vivo.

    #Hiroshi Kaneko, @Daigaku Hasegawa, @Tomohiro Itoyama, @Shinichiro Yoshida, @Atsushi Tomokiyo, @Sayuri Hamano, @Hideki Sugii, @Hidefumi Maeda.

    The 69th Annual Meeting of Japanese Association for Dental Research.  2021.10 

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    Event date: 2021.10

    Language:English  

    Country:Japan  

  • Nano Hydroxyapatite 含有 4-META/MMA-TBB レジンは、Calciumsensing receptor-MEK/ERK 経路を介してヒト歯髄幹細胞の象牙芽細胞様分化を促進する.

    @吉田晋一郎、@杉井英樹、@糸山知宏、#門脇正敬、#一法師啓太、#山下梢、@友清淳、@濱野さゆり、@長谷川大学、@前田英史.

    第 154 回日本歯科保存学会春季学術大会.  2021.6 

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    Event date: 2021.6

    Language:Japanese  

    Country:Japan  

  • 4-META/MMA-TBB resin including Nano-hydroxyapatite as a direct pulp capping material.

    @Shinichiro Yoshida, @Hideki Sugii, #Masataka Kadowaki, @Tomohiro Itoyama, @Daigaku Hasegawa, @Atsushi Tomokiyo, @Sayuri Hamano, #Keita Ipposhi, #Kozue Yamashita, @Hidefumi Maeda

    The 99th General Session & Exhibition of the IADR  2021.6 

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    Event date: 2021.6

    Language:English  

    Country:Japan  

  • 直接覆髄後の歯髄組織におけるM2マクロファージの集簇

    #門脇正敬,@吉田晋一郎,@糸山知宏,@友清淳,@濱野さゆり,長谷川大学,@杉井英樹,@前田英史

    2020.11 

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    Event date: 2020.11

    Language:Japanese  

    Country:Japan  

  • Decorinが未分化なヒト歯根膜細胞の骨芽細胞様分化に及ぼす影響

    #足立織利恵,@杉井英樹,@糸山知宏,@友清淳,@濱野さゆり,長谷川大学,@吉田晋一郎,#Mhd Safwan Albougha,@前田英史

    2020.11 

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    Event date: 2020.11

    Language:Japanese  

    Country:Japan  

  • シュワン細胞はヒト前骨芽細胞の骨芽細胞分化を促進する

    糸山知宏、吉田晋一郎、友清淳、長谷川大学、濱野さゆり、杉井英樹、小野太雅、藤野翔香、一法師啓太、前田英史.

    2019.11 

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    Event date: 2019.11

    Language:Japanese  

    Country:Japan  

  • GDNFは未分化なヒト歯根膜細胞のシュワン細胞様分化を誘導する

    糸山知宏、吉田晋一郎、友清 淳、長谷川大学、濱野さゆり、杉井英樹、有馬麻衣、野津 葵、和田尚久、前田英史.

    2018.11 

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    Event date: 2018.11

    Language:Japanese  

    Country:Japan  

  • 口腔顔面痛を予防するには? 歯内治療医からみた難治性歯痛 どんなケースをOFP専門医に相談するのか?

    糸山 知宏

    日本慢性疼痛学会プログラム・抄録集  2023.2  日本慢性疼痛学会

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  • ヒト歯周靱帯幹細胞由来のエキソソームはラットの頭蓋冠骨欠損に対して骨形成を促進する(Exosomes derived from human periodontal ligament stem cells promote bone formation of rat calvarial bone defects)

    Albougha Mhd Safwan, Sugii Hideki, Adachi Orie, Tomokiyo Atsushi, Hamano Sayuri, Hasegawa Daigaku, Obata Junko, Yoshida Shinichiro, Itoyama Tomohiro, Fujino Shoko, Kaneko Hiroshi, Maeda Hidefumi

    特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集  2022.5  (NPO)日本歯科保存学会

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    Language:English  

  • エトポシドによる老化誘導がヒト歯髄細胞の石灰化に及ぼす影響

    虎谷 渓, 糸山 知宏, 門脇 正敬, 前田 英史

    特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集  2024.4  (NPO)日本歯科保存学会

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  • Superoxide Dismutase 2の発現抑制が歯髄細胞の老化に及ぼす影響

    糸山 知宏, 前田 英史

    基礎老化研究  2023.6  日本基礎老化学会

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  • iPS細胞由来歯根膜幹細胞様細胞の分化誘導におけるFibrillin-2の役割

    濱野 さゆり, 山下 大輝, 糸山 知宏, 友清 淳, 長谷川 大学, 杉井 英樹, 兼子 大志, Mardini Bara, 前田 英史

    特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集  2023.5  (NPO)日本歯科保存学会

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  • Decorinによるヒト歯根膜幹細胞の骨芽細胞様分化誘導に関する分子機構の解析

    足立 織利恵, 杉井 英樹, 糸山 知宏, 兼子 大志, 友清 淳, 濱野 さゆり, 長谷川 大学, 小幡 純子, 吉田 晋一郎, 藤野 翔香, Albougha Mhd Safwan, 前田 英史

    特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集  2022.5  (NPO)日本歯科保存学会

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  • AhRシグナルの活性化はヒト歯根膜細胞のMMP12発現を亢進する

    門脇 正敬, 友清 淳, 糸山 知宏, 長谷川 大学, 濱野 さゆり, 杉井 英樹, 前田 英史

    特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集  2023.5  (NPO)日本歯科保存学会

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Professional Memberships

  • 日本歯科保存学会

  • 日本歯内療法学会

  • 日本基礎老化学会

Research Projects

  • 歯根膜幹細胞転換を応用した革新的歯周組織再生法の創出

    Grant number:24K12948  2024.4 - 2027.3

    科学研究費助成事業  基盤研究(C)

    長谷川 大学, 前田 英史, 杉井 英樹, 小幡 純子, 糸山 知宏, 兼子 大志

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    Grant type:Scientific research funding

    本研究では、MESTの発現を上昇させる“化合物”を探索し、その化合物を用いた新たな歯根膜幹細胞転換法、およびその化合物の直接応用による“効率的で簡便、かつ安全な歯周組織再生法”の確立を目指す。まず、オミックスデータ解析により歯根膜幹細胞転換を誘導する化合物を同定し、次にその化合物により誘導された歯根膜幹細胞様細胞の歯周組織再生能についてラット歯周組織欠損モデルを用いて評価する。さらに、この化合物の組織欠損部への直接応用が歯周組織再生に及ぼす影響についても検討を行う。

    CiNii Research

  • PAI-1に着目した、歯髄細胞の老化とそれに伴う石灰化の促進の機序の解明

    Grant number:24K19898  2024.4 - 2026.3

    科学研究費助成事業  若手研究

    糸山 知宏

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    Grant type:Scientific research funding

    超高齢社会において、残存歯数を保つことは国民のQOLの向上につながる。歯の加齢変化として、第二象牙質の添加、歯髄結石の形成といった歯の内部で石灰化が亢進し、根管治療が困難になる。PAI-1は、象牙芽細胞様分化を亢進することが明らかにされており、さらに老化細胞では、老化マーカーであるp53によって発現が上昇し、細胞老化を促進する因子であることが報告されている。そこで本研究では、歯髄細胞の老化と石灰化におけるPAI-1の役割を解析し、歯髄細胞における細胞老化とそれに伴う石灰化の亢進のメカニズムを明らかにしていく。

    CiNii Research

  • ナノハイドロキシアパタイト含有レジンを応用した歯周組織再生誘導型歯質接着材の開発

    Grant number:23K09171  2023.4 - 2026.3

    科学研究費助成事業  基盤研究(C)

    杉井 英樹, 前田 英史, 友清 淳, 長谷川 大学, 濱野 さゆり, 糸山 知宏, 兼子 大志, 藤野 翔香, 小幡 純子

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    Grant type:Scientific research funding

    naHAp/SBがセメント質・歯根膜誘導型の新規材料として、破折歯の治療に応用できるとの仮説を立て、naHAp/SBを破折歯の治療に応用し、naHAp/SBのセメント質・歯根膜再生能を明らかにすることを計画した。まず、in vitroにて、歯根膜幹細胞(PDLSCs)を用いて、naHAp/SBが、その歯質接着能、生体親和性、機械的強度、および生体活性(セメント質および歯根膜形成誘導能)に及ぼす影響について解析を行う。その後、in vivoにて、当研究室で樹立したラット歯周組織欠損モデルを応用し、ラット臼歯部歯根破折歯モデルを作製する。同動物モデルを用いて、破折部周囲の組織の治癒状態を検証する。

    CiNii Research

  • To elucidate the mechanism of senescence of dentin-pulp complex targeting the oxidative stress.

    Grant number:23K24528  2022.4 - 2026.3

    Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (B)

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    Grant type:Scientific research funding

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  • 歯周組織発生過程の模倣を基盤とするハイブリッド構造体の創成と歯周組織再生への応用

    Grant number:23K24529  2022.4 - 2026.3

    科学研究費助成事業  基盤研究(B)

    友清 淳, 前田 英史, 糸山 知宏, 和田 尚久, 小幡 純子, 杉井 英樹, 藤野 翔香

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    Grant type:Scientific research funding

    歯周組織は神経堤細胞 (NC) に発生を由来することから、NCは歯周組織の発生過程を模倣する上で極めて重要な細胞であると考えられる。申請者らは人工多能性幹細胞 (iPSC) からNC様細胞 (iPSC-NC) への分化誘導法、およびiPSC-NCから歯根膜幹細胞様細胞 (iPSC-PDLSC) への分化誘導法を確立している。そこで本研究では、これらの細胞に対するゲノム解析結果から同定した、iPSC-NCからiPSC-PDLSCへの分化を誘導する形態形成因子および足場材について至適化を行い、その結果を基にiPSC-NC/形態形成因子/足場材から構成されるハイブリッド構造体を作製する。

    CiNii Research

  • ドーパミンの下流のシグナル経路による象牙質形成機構の解明

    Grant number:22K17061  2022 - 2023

    日本学術振興会  科学研究費助成事業  若手研究

    糸山 知宏

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    Authorship:Principal investigator  Grant type:Scientific research funding

    高齢者の歯の変化として、第二・第三象牙質の添加による歯髄腔の狭窄が挙げられる。老年人口の増加から、根管が狭窄した治療困難な歯は、今後も増加していくと考えられ、歯髄腔狭窄のメカニズムの解明が求められる。これまでに申請者らは、ラットの象牙芽細胞層においてドーパミンおよびドーパミン受容体が発現し、さらにドーパミンが、前象牙芽細胞の象牙芽細胞関連因子の発現と石灰化物形成を促進することを報告している。そこで本研究では、象牙芽細胞においてドーパミンが象牙質を形成していると仮説を立て、その下流のシグナルとその制御機構を詳細に検討することで、第二・第三象牙質形成の制御機構のメカニズムの解析を目的としている。

    CiNii Research

  • Development of a new drug for periodontal regeneration based on the activation of neural crest cells

    Grant number:21K19608  2021.7 - 2024.3

    Grants-in-Aid for Scientific Research  Grant-in-Aid for Challenging Research (Exploratory)

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    Grant type:Scientific research funding

    CiNii Research

  • 転写因子PAX9を標的とした歯根膜幹細胞誘導因子の同定

    Grant number:21K09876  2021.4 - 2024.3

    科学研究費助成事業  基盤研究(C)

    濱野 さゆり, 前田 英史, 吉田 晋一郎, 糸山 知宏, 藤野 翔香, 小幡 純子, 杉井 英樹

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    Grant type:Scientific research funding

    HPDLCが産生する多数の細胞外基質から、歯根膜幹細胞誘導因子を同定することは困難である。そこで申請書らは、歯根膜幹細胞誘導因子の産生を制御する転写因子に着目し、HPDLCにおける遺伝子発現の網羅的解析を用いてPAX9を抽出した。本研究では、PAX9が歯根膜幹細胞誘導に及ぼす影響について検討し、その下流にある歯根膜幹細胞誘導因子を同定することを目的とした。本研究は、iPS細胞から多量の歯根膜幹細胞の獲得する方法を明らかにし、それを応用した新規歯周組織再生療法の開発研究に繋がるものである。

    CiNii Research

  • シュワン細胞由来の因子を用いた新規歯周組織再生療法の開発

    Grant number:20K23115  2020 - 2021

    日本学術振興会  科学研究費助成事業  研究活動スタート支援

    糸山 知宏

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    Authorship:Principal investigator  Grant type:Scientific research funding

    重度の歯周炎や外傷等により歯周組織に重篤な傷害が生じた場合、骨および歯根膜組織を含む歯周組織の再生は困難となり、抜歯に至る可能性が高くなる。したがって、広範囲に喪失した歯周組織の再生を可能にする治療法の開発が待ち望まれている。これまでに申請者らは、末梢組織再生に重要な役割を果たすシュワン細胞が、傷害を受けた歯周組織に集簇すること、そしてシュワン細胞は、前骨芽細胞および歯根膜幹細胞の骨芽細胞分化を促進する因子を分泌することを報告している。そこで本研究では、このような因子を同定し、歯の長期保存が可能となる新規歯周組織再生療法を開発することを目的としている。

    CiNii Research

  • シュワン細胞由来の因子を用いた新規歯周療法の開発

    Grant number:GAG0K23115  2020 - 2021

    日本学術振興会  科学研究費助成事業  研究活動スタート支援

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    Authorship:Principal investigator  Grant type:Scientific research funding

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Class subject

  • 臨床実習

    2023.4 - 2024.3   Full year

  • 歯科保存学Ⅰ

    2023.4 - 2023.9   First semester

  • 臨床予備実習

    2023.4 - 2023.9   First semester

  • 臨床実習

    2022.4 - 2023.3   Full year

  • 臨床予備実習

    2022.4 - 2022.9   First semester

  • リサーチエクスポージャ

    2021.6 - 2021.8   Summer quarter

  • 臨床実習

    2021.4 - 2022.3   Full year

  • アーリーエクスポージャ

    2021.4 - 2021.6   Spring quarter

  • リサーチエクスポージャ

    2020.10 - 2021.3   Second semester

  • 臨床実習

    2020.10 - 2021.3   Second semester

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FD Participation

  • 2024.9   Role:Participation   Title:増える認知症と対策(福岡市

    Organizer:[Undergraduate school/graduate school/graduate faculty]

  • 2024.7   Role:Participation   Title:入学者選抜における面接試験~位置付けと評価方法について

    Organizer:Undergraduate school department

  • 2024.6   Role:Participation   Title:本学の国家試験への取り組み

    Organizer:Undergraduate school department

  • 2023.6   Role:Participation   Title:科学研究費補助金採択率向上に向けた工夫

    Organizer:[Undergraduate school/graduate school/graduate faculty]

  • 2020.11   Title:令和2年度馬出地区4部局合同男女共同参画FD

Outline of Social Contribution and International Cooperation activities

  • 一般歯科医院にて兼業を行っており、地域医療に貢献している。

Specialized clinical area

  • Biology / Medicine, Dentistry and Pharmacy / Dentistry / Conservative Dentistry

Year of medical license acquisition

  • 2015

Notable Clinical Activities

  • 従来の材料および方法を用いた歯内治療に加えて、マイクロスコープやニッケルチタンファイル、垂直加圧根管充填等の歯内治療の専門的な器具を積極的に取り入れ、一本の歯の保存にこだわり診療に取り組んでいる。穿孔やファイル破折といった偶発症や、垂直性歯根破折や歯根吸収、歯内-歯周疾患といった難症例にも、MTAセメントや意図的再植術を用いて治療を行っており、抜歯と判断された歯を保存している。  また、難治性の症例の中には、根管治療を行っても症状が改善しない慢性疼痛のものや、歯以外に原因がある非歯原性疼痛といった症例が多く含まれている。そういった症例を早期診断し、口腔顔面痛外来に相談することによって、患者のQOLの向上に貢献できるように取り組んでいる。