Updated on 2024/12/10

Information

 

写真a

 
NAKANO KOJI
 
Organization
Faculty of Engineering Department of Applied Chemistry Associate Professor
Center for Future Chemistry (Concurrent)
School of Engineering (Concurrent)
Graduate School of Engineering (Concurrent)
Title
Associate Professor
Contact information
メールアドレス
Tel
0928022890
Profile
1. DNA biosensor and gene sensor 2. Bioelectrochemistry of redox enzymes and peptides 3. DNA nanomaterials for electrochemical sensing and electrical device applications 4. Synthesis and bioanalytical applications of artificial peptides, peptide enzymes, and nucleic acid analogues 5. Synthesis of new carbon quantum dots for biosensing and bioimaging applications
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Degree

  • Doctor of Engineering

Research History

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Research Interests・Research Keywords

  • Research theme: Chemical Synthesis of Microperoxidases and Their Structural/Functional Modifications for Application to Nanobiosensors

    Keyword: Bioelectrochemistry, Proteins, Enzymes, Biosensor

    Research period: 2015.10

  • Research theme: PNAzyme: Peptide nucleic acid enzyme as a new bioanalysis platform

    Keyword: Peptide nucleic acid, Biosensor, Bioimaging

    Research period: 2015.4

  • Research theme: Synthesis of novel nucleic acid synthesis substrates containing redox-active tag for electrochemical bioassay of nucleic acids

    Keyword: Nucleic acid, Substrate for nucleic acid synthesis, Redox-active tag, Bioassay

    Research period: 2015.4

  • Research theme: Pyrrole-imidazole polyamide capable of photon up conversion for in vivo imaging of double-helical DNA

    Keyword: Pyrrole-imidazole polyamide, Photon up conversion, Bioimaging

    Research period: 2015.4

  • Research theme: Synthesis of chemically-modified carbondots for application to biosensing and bioimaging

    Keyword: Carbondot, Nanocluster, Fluorescence, Biosensor, Bioimaging

    Research period: 2013.4

  • Research theme: Biosnsesing and bioimaging of odor molecules using synthetic polypeptide host molecules

    Keyword: Biosensor, Bioimsging, Nanoreporter particle. Odor molecule

    Research period: 2011.10

  • Research theme: DNA Supramolecular Conjugate for Nanobiosensing Application

    Keyword: Biosensor, Nanobiodevice, DNA, Supramolecular Architecture

    Research period: 2000.10

  • Research theme: Atomic Force Microscopy Imaging of Biomacromolecules for Chemical Sensing Applications based on Single-Molecule Strategy

    Keyword: AFM, Bioimaging, Analytical Chemistry, Single molecule

    Research period: 2000.10

  • Research theme: Direct Electrochemistry of Proteins and Enzymes Attachmed on Self-Assembled Monolayer Electrode and Biosensor Application.

    Keyword: Bioelectrochemistry, Proteins, Enzymes, Biosensor

    Research period: 1995.10 - 2020.3

  • Research theme: Development of DNA biosensor and gene sensor

    Keyword: DNA, Gene, Biosensor, Scanning Electrochemical Microscopy, High-Throughput Assay

    Research period: 1992.4 - 2020.3

  • Research theme: Supramolecular Organizate for Chemical Analysisi Applications

    Keyword: Molecular Organizate, Synthetic Bilayer Membrane, Self-Assembled Monolayer, Multi-Phase Polymer Material

    Research period: 1985.4

Awards

  • フローインジェクション分析論文賞

    2013.11   日本分析化学会・フローインジェクション分析研究懇談会   下記の研究業績により同賞を受賞した。 Chemiluminescence Immunoassay for a Nonionic Surfactant using a Compact Disc-type Microfluidic Platform 郭 帥、中野幸二、中嶋 秀、内山一美、辺見彰秀、山崎吉一、諸岡成治、石松亮一、今任稔彦

  • フローインジェクション分析論文賞

    2006.6   日本分析化学会・フローインジェクション分析研究懇談会   下記学術論文が褒賞の対象となった。 Electrochemical Immunoassay for Vitellogenin Based on Sequential Injection Using Antigen-immobilized Magnetic Microbeads. Koji Hirakawa, Masaaki Katayama, Nobuaki Soh, Koji Nakano, and Toshihiko Imato, Analytical Sciences, 22 (1), 81-86 (2006).

  • 奨励賞

    1996.9   日本分析化学会   Study of Methods for Electrochemcial Analysis based on Molecular Organizates as Chemical Transducer Material

  • 若手研究論文「化学のフロンティアIX」採択

    1993.2   日本化学会   Study of Methods for Chemcial Analysis based on Molecular Organizates as Transducer Material

Papers

  • Covalent Hyperbranched Polymer Self-Assemblies of Three-Way Junction DNA for Single-Molecule Devices Reviewed International journal

    K. Nakano, #T. Sawada, #Y. Mori, #K. Morita, R. Ishimatsu

    Langmuir   36 ( 34 )   10166 - 10174   2020.9

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    Language:Japanese   Publishing type:Research paper (scientific journal)  

    A hyperbranched polymer (HBP) made of three-way junction (TWJ) DNAs is reported. Three types of 26-mer DNAs with 5′-ends modified with psoralen (PSN) were synthesized. All had self-complementary sequences starting from the 5′-end to the sixth base (AAGCTT), allowing intermolecular hybridization. The base sequences of the remaining 20-mer sites were designed so that upon hybridization, three strands had a TWJ structure with a mass of 25,000 that could be further grown by forming HBPs. PSN photochemically reacts to form interstrand cross-links that increase the polymer stability. Aggregates [(380 ± 44) nm and (65 ± 6) nm] detected with dynamic light scattering for TWJ-DNA solutions were also imaged by electron microscopy and atomic force microscopy, providing evidence of hyperbranched polymerization. The TWJ unit also polymerized on solid substrates such as Au and glass and formed self-assembled monolayers (SAMs). The HBP SAMs were integrated into commercial Pt-interdigitated electrode arrays. The DNA devices had current–voltage curves typical of metal–insulator–metal Schottky diodes; the effective barrier heights and the ideality factors were 0.52 ± 0.002 eV and 21 ± 3.2, respectively. The series resistances were (26 ± 3.3) × 10^6 Ω, which may provide insights into DNA electron transport. The DNA HBP enables stable electrical connections with probe electrodes and will be an important single-molecule platform.

    DOI: https://dx.doi.org/10.1021/acs.langmuir.0c01621

    Repository Public URL: https://hdl.handle.net/2324/7236930

  • Folding and Assembly of Vanilloid Receptor Secondary-Structure Peptide with Hexahistidine Linker at Nickel−Nitrilotriacetic Acid Monolayer for Capsaicin Recognition Reviewed International journal

    K. Nakano, #J. Horiuchi, #S. Hirata, #M. Yamanaka, #H. Himeno, R. Ishimatsu

    Langmuir   35 ( 6 )   2047 - 2054   2019.2

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    Herein, we report the self-assembly of a synthetic vanilloid receptor (VR) peptide that selectively
    binds capsaicin. We synthesized a 26-mer peptide—YSEILFFVQS-HHHHHH-LAMGWTNMLY (S3HS4)—comprising two chemoreceptor domains of transient receptor potential channel (TRPV1) linked by a hexahistidine sequence. High-speed atomic force microscopy (AFM) imaging in water revealed that the peptide structures alternated rapidly between wedge shape and linear forms. Circular dichroism spectroscopy showed that 65% of the amide units in the peptide chain adopted an α-helix structure, which was ascribed to the chemoreceptor domains. S3HS4 developed well-packed monolayers at the Ni-treated thiolated nitrilotriacetic acid self-assembled monolayers by chelation of the hexahistidine segment, as characterized by infrared spectroscopy and AFM, which exhibited statistically constant specific height. Therefore, S3HS4 was expected to fold spontaneously upon chelation, and the resulting helix−turn−helix conformers developed films while uniformly oriented: the tilt angle was 69° from the surface normal to the substrate. According to microgravimetric analysis using a quartz crystal microbalance (QCM), the adsorption was 84 ± 47 pmol cm−2 (n = 3), which was almost consistent with the saturation adsorption of an α-helix unit. We also used a QCM to investigate the host−guest reactions of S3HS4 and found that the S3HS4-attached QCM-chip-bound capsaicin with an apparent binding constant of (4.2 ± 3.6) × 104 M−1 (n = 4), whereas there was no evidence of binding to vanillin or acetophenone. Two controls—a blank chip without S3HS4 and a chip modified with a single helical peptide (LAMGWTNMLY-HHHHHH)—produced no capsaicin response. To the best of our knowledge, S3HS4 is the first example of a synthetic VR mimic peptide. We believe that the present surface-directed structure-based design can be used to exploit the α-helix bundle in hexahistidine-linked bishelical peptides.

    DOI: DOI: 10.1021/acs.langmuir.8b03202

    Repository Public URL: https://hdl.handle.net/2324/7237126

  • Totally Synthetic Microperoxidase-11 Reviewed International journal

    #J. Tanabe, K. Nakano, R. Ishimatsu, T. IMATO, @H. Okabe, @N. Matsuda

    Royal Society Open Science   5 ( 3 )   172311 - 172320   2018.5

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    Language:Japanese   Publishing type:Research paper (scientific journal)  

    A totally synthetic microperoxidase-11 (MP-11) is reported. Accordingly, the undecapeptide (VQKCAQCHTVE) was synthesized by solid-phase peptide synthesis followed by the thiol-ene click reaction with haemin for reconstitution. High-speed atomic force microscopy measurement conducted in water confirmed the protein reconstitution by visualizing the morphological differences as animated molecular images. The synthetic MP-11 showed a considerable magnitude of catalytic activity (27%) against the natural MP-11 in the oxidation of 3,3′,5,5′-tetramethylbenzidine by hydrogen peroxide, whereas it showed very low (2.7%) activity of a synthetic variant with a point mutation (VQKCAQCMTVE, H8M). Slab waveguide spectroscopic measurements revealed that the ferrous/ferric redox reaction occurred by the direct electron transfer with specific spectral changes. Indeed, if hydrogen peroxide existed in the solution phase, the peroxidase-modified electrode showed catalytic current–voltage behaviour regardless of whether it was prepared using natural MP-11 or the synthetic MP-11. If a substrate recycling reaction was assumed, computer simulation well reproduced the experimental curves to give a global set of electrocatalytic reaction parameters. In any of the experiments, the synthetic MP-11 and natural MP-11 gave almost identical results. Our approach will be a convenient means of preparing MP-11, as well as its mutants, that does not rely on nature.

    DOI: http://dx.doi.org/10.1098/rsos.172311

    Repository Public URL: https://hdl.handle.net/2324/7238341

  • Carbon quantum dots as fluorescent component in peroxyoxalate chemiluminescence for hydrogen peroxide determination Reviewed

    Koji Nakano, #Takayuki Honda, #Kanako Yamasaki, #Yoshiki Tanaka, #Keiichi Taniguchi, Ryoichi Ishimatsu, Toshihiko Imato

    Bulletin of the Chemical Society of Japan   91 ( 7 )   1128 - 1130   2018.1

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    Nitrogen-doped carbon quantum dots synthesized by onepot, microwave-assisted pyrolysis of citric acid in the presence of a small number of N-doping precursors, 1, 2-ethylenediamine, were found to be involved in the chemically initiated electron exchange luminescence enabling peroxyoxalate chemiluminescence assay of hydrogen peroxide in the concentration range of 101000 μM.

    DOI: 10.1246/bcsj.20180095

    Repository Public URL: https://hdl.handle.net/2324/7238315

  • Monolithic Peptide-Nucleic Acid Hybrid Functioning as an Artificial Microperoxidase Reviewed International journal

    K. Nakano, #J. Tanabe, R. Ishimatsu, T. IMATO

    Bioconjugate Chemistry   28 ( 5 )   2031 - 2034   2017.5

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    A new peptide nucleic acid (PNA) with an installed peroxidase function has been developed. Fmoc solid phase peptide synthesis prepared a PNA hy-brid (VQKCAQCHTVE-(C2H4O)2CH2-[PNA(T)]6-G) that renders the microperoxidase backbone, fol-lowed by reconstitution with hemin. The resulting holocompound catalyzed the oxidation of 3,3',5,5'-tetramthylbenzidine by H2O2 to 50% that of natural microperoxidase-11, whereas the apo-form and hemin gave no responses. The peroxidase domain was found to be active toward direct electrochemis-try and the PNA hybrid served for gene sensor; in the presence of the target DNA (5'-CATGTATAAAAAA-3'), an electrode-attached DNA probe (5'-TsTsTsTsTsTCTCATACATG-3') showed the ferric-to-ferrous quasi-reversible wave (–276 mV vs. Ag/AgCl) through sandwich hybridi-zation. Moreover, the hybridization product could accept H2O2 as an oxidant to enhance the reduction current, which occurred likely based on the iron(II)-center-recycling with specific rate constant of 0.19 s–1.

    DOI: https://doi.org/10.1021/acs.bioconjchem.7b00216

    Repository Public URL: https://hdl.handle.net/2324/7238384

  • Synthesis and Self-Assembly of His-tag Hybrid of Substrate-Binging Short Domain in Transient Receptor Potential Vanilloid Type 1 for Vanaillin Sensing Application Reviewed International journal

    Koji Nakano, Shingo Hirata, Jun Horiuchi, Ryoichi Ishimatsu, Toshihiko Imato, Takeshi Onodera, Kenshi Hayashi

    Transactions of the Materials Research Society of Japan   40 ( 2 )   175 - 178   2015.7

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    DOI: http://doi.org/10.14723/tmrsj.40.175

    Repository Public URL: https://hdl.handle.net/2324/7239370

  • Potentiometric DNA Sensing Platform Using Redox-active DNA Probe Pair for Sandwich-type Dual Hybridization at Indicator Electrode Surface Reviewed International journal

    Koji Nakano, Takayuki Kimura, Yosuke Kitamura, Toshihiro Ihara, Ryoichi Ishimatsu, Toshihiko Imato

    Journal of Electroanalytical Chemistry   720-721   71 - 75   2014.4

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    A potentiometric sensing platform that enables real-time DNA hybridization detection is described. A model target DNA, t37s: 5'AAA AAA AAA AAA-(TC)2-Ts5-(TC)2-GGA GCT GGT GGC3', which consist of a dodecamer polydeoxyadenylic acid and the human K-ras oncogene, and a five-successive deoxythymidine phosphorothioate (Ts) was designed. With the gold–phosphorothioate binding, the DNA could bind the complementarily sequences to concentrate them at the gold electrode surfaces. Accordingly, a dodecamer polydeoxythymidylic acid having ferroin-moiety (T12FeP) and a ferrocene-modified complementary of K-ras (KrasFc) were synthesized. Electrochemical quartz-crystal-microbalance (QCM) using Au-sputtered quartz chips that served as the indicator electrode collected the electrode responses. When the electrode surface was treated with the T12FeP-hybridizad t37s, which were subsequently oxidized to the corresponding Fe(III) form, the emf developed in a buffer solution responded to KrasFc; with the electrode-attached t37s the redox-active DNAs could group into a pair to establish specific redox-titration equilibrium. A QCM confirmed the on-electrode titrimetry feasible by determining the initial concentration and the amount of hybridization along with the emf measurements. Although the present method is necessary for two-kinds of redox conjugation of the target DNA, it should be important as a novel framework of electrochemical gene sensing. Preliminary examples of real-time measurement were also demonstrated with the results of kinetics analysis data.

    DOI: http://dx.doi.org/10.1016/j.jelechem.2014.03.029

    Repository Public URL: https://hdl.handle.net/2324/7325071

  • 光架橋性オリゴヌクレオチドからのDNAデンドリマー自己組織化膜形成

    中野 幸二, 澤田 貴文, 森 美詞, 石松 亮一, 今任 稔彦

    電子情報通信学会技術研究報告   112 ( 456 )   29 - 32   2013.3

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    We designed three types of 26-mer oligodeoxyribonucleotides that produce the three-way junction (TWJ) structure through the selective hydrogen bonding between the complimentary base-pairs. At the 5'-end was left a self-complimentary sticky sequence, which was further modified with psoralen (7H-furo[3,2-g]chromen-7-one)to exert covalent molecular aggregation through the interstrand photochemical crosslinking. Dynamic light scattering as well as high-resolution electron microscopy revealed that the TWJ unit developed very large aggregates in solution, a new kind of dendrimer made by the TWJ-DNA duplex, which were ca. 400-nm in diameter having three-dimensional network structure. We further examined the TWJ-DNA molecular aggregation at the solid substrates. Interestingly, we found that the TWJ-DNA duplexes self-assembled to developed uniform dendrimer monolayers. Various surface analysis methods including cyclic voltammetry and electrochemical impedance spectroscopy characterized the DNA dendrimer SAMs in detail.

  • A Pivot-Hinge-Style DNA Immobilization Method with Adaptable Surface Concentration Based on Oligodeoxynucleotide-Phosphorothioate Chemisorption on Gold Surfaces Reviewed International journal

    Hisao Yoshinaga, Koji Nakano, Nobuaki Soh, Ishimatsu Ryoichi, Toshihiko Imato

    Anal. Sci.   28 ( 11 )   1059 - 1064   2012.11

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    DOI: https://doi.org/10.2116/analsci.28.1059

    Repository Public URL: https://hdl.handle.net/2324/7325019

  • AFM-Imaging Diagonosis Method for Single Nucleotide Polymorphism Using Molecular Beacon DNA as an Intramolecullar Ligation Template of Target DNA and a Viewable Indicator Reviewed International journal

    Hisao Yoshinaga, Koji Nakano, Nobuaki Soh, Toshihiko Imato

    Anal. Sci.   28 ( 10 )   939 - 945   2012.10

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    DOI: https://doi.org/10.2116/analsci.28.939

    Repository Public URL: https://hdl.handle.net/2324/7325018

  • Transglutaminase-Mediated in Situ Hybridization (TransISH) System: A New Methodology for Simplified mRNA Detection Reviewed International journal

    北岡 桃子, Masayuki Mitsumori, Kounosuke Hayashi, Yoshiyuki Hiraishi, Hisao Yoshinaga, Koji Nakano, Katsuyuki Miyawaki, Sumihare Noji, Masahiro Goto, Noriho Kamiya

    Anal. Chem.   84 ( 14 )   5885 - 5891   2012.7

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    DOI: https://doi.org/10.1021/ac2034198

    Repository Public URL: https://hdl.handle.net/2324/7325017

  • Programmable protein–protein conjugation via DNA-based self-assembly Reviewed International journal

    Josui Shimada, Tatsuo Maruyama, 北岡 桃子, Hisao Yoshinaga, Koji Nakano, Noriho Kamiya, Masahiro Goto

    Chem. Commun.   48 ( 50 )   6226 - 6228   2012.6

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    DOI: https://doi.org/10.1039/C2CC30618B

    Repository Public URL: https://hdl.handle.net/2324/7325016

  • アルカンチオール単分子膜修飾電極に固定化したチトクロームcの直接電子移動反応の研究

    中野幸二,吉武忠輝,山下泰徳,宗 伸明,今任稔彦

    九州大学中央分析センター報告   27   2010.12

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  • Label-free DNA Detection Platform Based on Atomic Force Microscopy Visualisation: Characterising the Molecular-Recognition-Triggered Conformational Changes of an Immobilised Receptor Oligonucleotide Probe Reviewed International journal

    Koji Nakano, Hirokazu Yamanouchi, Hisao Yoshinaga, Nobuaki Soh, Toshihiko Imato

    Chem. Commun.   46 ( 13 )   5683 - 5685   2010.6

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    Using atomic microscopy imaging, probe DNA sequence self-assemblies developed on Si(100) substrates undergo a conformational transition from an extended stem-loop structure to a double helix; such assemblies readily report on DNA molecular recognition events and should be suitable as a label-free, DNA hybridisation assay platform.

    DOI: https://doi.org/10.1039/C002642E

  • An Atomic Force Microscopy Assay of Intercalation Binding, Unwinding and Elongation of DNA, Using a Water-Soluble Psoralen Derivative as a Covalent Binding Probe Molecule Reviewed International journal

    Koji Nakano, Yosuke Katsumi, Nobuaki Soh, Toshihiko Imato

    Bull. Chem. Soc. Jpn   2010 ( 3 )   273 - 275   2010.3

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    A single-molecule strategy using atomic force microscopy has simply yet robustly probed an intercalation binding
    related specific structural relaxation of covalently closed
    circular pBR322 DNA as well as double-strand elongation in
    its linear form by taking advantage of a new psoralen
    derivative, N,N,N-trimethyl-1-(2,5,9-trimethyl-7-oxo-7Hfuro[3,2-g]chromen-3-yl)methanaminium chloride, that covalently binds to DNA through a photo-crosslinking reaction.

    DOI: https://doi.org/10.1246/bcsj.20090166

    Repository Public URL: https://hdl.handle.net/2324/7324488

  • Synthesis Of Circular Double-Stranded DNA Having Single-Stranded Recognition Sequence As Molecular-Physical Probe For Nucleic Acid Hybridization Detection Based On Atomic Force Microscopy Imaging Reviewed International journal

    Koji NAKANO, Hideshi MATSUNAGA, Masaharu MURATA, Nobuaki SOH, Toshihiko IMATO

    Anal. Sci.   25 ( 8 )   993 - 998   2009.8

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    A new class of DNA probes having a mechanically detectable tag is reported. The DNA probe, which consists of a
    single-stranded recognition sequence and a double-stranded circular DNA entity, was prepared by polymerase reaction.
    M13mp18 single strand and a 32mer oligodeoxynucleotide whose 5`-end is decorated with the recognition sequence were
    used in combination as template and primer, respectively. We have successfully demonstrated that the DNA probe is
    useful for bioanalytical purposes: by deliberately attaching target DNA molecules onto Au(111) substrates and by
    mechanically reading out the tag-entity using a high-resolution microscopy including atomic force microscopy,
    visualization/detection of the individual target/probe DNA conjugate was possible simply yet straightforwardly. The
    present DNA probe can be characterized as a 100%-nucleic acid product material. It is simply available by one-pod
    synthesis. A surface topology parameter, image roughness, has witnessed its importance as a quantitative analysis index
    with particular usability in the present visualization/detection method.

    DOI: DOI: 10.2116/analsci.25.993

    Repository Public URL: https://hdl.handle.net/2324/7324481

  • Positive-feedback-mode scanning electrochemical microscopy imaging of redox-active DNA-poly(1,4-benzoquinone) conjugate deposited on carbon electrode for micrometer-sized hybridization biosensor applications Reviewed International journal

    K. Nakano, K. Nakamura, K. Iwamoto, N. Soh, T. Imato

    J. Electroanal. Chem.   628 ( 1-2 )   113 - 118   2009.2

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    Scanning electrochemical microscopy of DNA microdots deposited on carbon fiber microelectrodes (diameter, 33 μm) has been demonstrated. The microdots, which comprised quinone polymer matrices with capture probe (CP) DNA grafted onto them, can report hybridization events via changes in their electrochemical reactivity. Furthermore, the polymer matrices, even after conjugation with CP DNA, possess a certain degree of charge-transport capability and thus allow for positive-feedback-mode imaging. We have successfully obtained well-resolved micrometer-sized dot images (diameter, 60–100 μm) of the microelectrodes: they generate a considerable magnitude of current rise over 10 nA while they gave a current decrease, typically 1 nA, in responding the hybridization event at the CP DNA. The sensor response was found to fall a little larger than the background current (0.6–0.8 nA). However, the particular SECM measurement system represented good signal-to-noise ratio reliably allowing the detection of DNA hybridization feasible. Obtaining these results, we have concluded that the particular DNA-modified electrode with SECM detection should be useful for readout of DNA hybridization sensor coupled with a high-throughput-device such as DNA microarrays.

    DOI: https://doi.org/10.1016/j.jelechem.2009.01.014

    Repository Public URL: https://hdl.handle.net/2324/7324478

  • Synthesis of 1,4-hydroquinone-terminated alkanethiol and self-assembly on gold as characterized by interfacial electrochemistry, electrocatalysis application and ab initio calculation based on comparison with catechol-presenting analogue. Reviewed International journal

    K. Nakano, K. Ohkubo, H. Taira, M. Takagi, N. Soh, T. Imato

    J. Electroanal. Chem.   623 ( 1 )   49 - 53   2008.11

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    Synthesis and self-assembly of a mercaptoundecaneamide derivative having a terminus of 1,4-hydroquinone (QT) are described. Electrochemical measurements on the QT-modified Au electrode revealed that the alkanethiol compound undergoes self-assembly to exhibit specific electrochemical activity originates from the reversible quinone/hydroquinone redox reaction at the terminus. We have achieved to obtain the electrochemical active surface coverage (0.11 nmol cm−2), formal potential (+246 mV, pH 3, Ag/AgCl) that changes pH-dependently (58 mV per pH) and also a set of the electron transfer reaction parameters, all of which were consistent with those of the previously reported structural isomer, catechol-terminated mercaptoundecaneamide (CT). Contrastingly, we found that these alkanethiol monolayers give marked contrast in an elecrocatalysis application: the CT-monolayer electrodes showed electrocatalytic capability in oxidation of NADH solution species while the QT-monolayer electrodes did not at all. By comparing some results of theoretical approach, we have attributed the surface selectivity to the spatiality of particular molecular orbital in the catalysis molecule. This observation should be important as an example of spatiality–reactivity relationships in a molecular design of chemically modified electrode.

    DOI: https://doi.org/10.1016/j.jelechem.2008.06.016

    Repository Public URL: https://hdl.handle.net/2324/7324475

  • Electrocatalytic oxidation of dihydronicotineamide adenine dinucleotide on gold electrode modified with catechol-terminated alkanethiol self-assembly Reviewed International journal

    K. Nakano, K. Ohkubo, H. Taira, M. Takagi, T. Imato

    Anal. Chim. Acta   619 ( 1 )   30 - 36   2008.6

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    Synthesis of a mercaptoundecaneamide derivative having a terminus of catechol is described. FT-IR spectroscopic characterization showed that the new molecular entry simply undergoes molecular self-assembly on Au substrate surfaces promoting intra- and intermolecular hydrogen bonds to form well-packed monolayers. Cyclic voltammetric (CV) measurements on the monolayer-modified Au electrode revealed that the surface adlayer possesses specific electrochemical activity due to the reversible catechol/o-quinone redox reaction having characteristics of a surface process and also pH-dependence in its formal potential (59 mV per pH). Detailed analysis of CVs gave fundamental electrochemical parameters including the electroactive surface coverage (0.20–0.24 nmol cm−2), the transfer coefficients (0.24 in oxidation and 0.81 in reduction), and also the electron transfer rate constant (1.10–2.76 s−1). These data were almost consistent to those seen in literature. We have also found that the catechol monolayer modified electrode exhibits an electrocatalytic function in NADH oxidation. That is, the faradaic current appeared reinforcingly at around the same potential where catechol function is oxidized in the monolayer and increased with an increase in the NADH concentration from 1 to 5 mM, and then reached to a plateau indicating a catalyzed reaction pathway. Detailed analyses revealed that the present system could be characterized by its weak stability of the intermediate compound formed and prompt reaction rate compared with the previously reported chemically modified electrode (CME) systems. We think this type of achievement should be important for the basics of biosensors that rely on dehydrogenase enzymes.

    DOI: https://doi.org/10.1016/j.aca.2008.02.009

    Repository Public URL: https://hdl.handle.net/2324/7324471

  • Phospholipid-linked Coumarin: A Fluorescent Probe for Sensing Hydroxyl Radicals in Lipid Membrane Reviewed

    N. Soh, K. Makihara, T. Ariyoshi, D. Seto, T. Maki, H. Nakajima, K. Nakano, T. Imato

    Analytica Sciences   24 ( 2 )   293 - 296   2008.2

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    A fluorescent probe, DPPEC (1,2-dipalmitoylglycerophosphorylethanolamine labeled with coumarin) was developed for detecting hydroxyl radical (·OH) in lipid membranes. The coumarin moiety contributes to the fluorescent detection of ·OH and the phospholipids moiety gives a driving force to localize the probe in lipid membranes. DPPEC in liposomal membranes rapidly reacted with ·OH and increased the fluorescence intensity, depending on the concentration of ·OH. The increase in the fluorescence intensity induced by ·OH was effectively suppressed by the addition of DMSO. The probe exhibited a higher fluorescence response to ·OH over other reactive oxygen species, such as hydrogen peroxide, nitric oxide, peroxynitrite, alkylperoxyl radical, and hypochlorite. DPPEC would be useful as a new type of fluorescent probe that can localize in lipid membranes and detect ·OH efficiently.

    DOI: https://doi.org/10.2116/analsci.24.293

    Repository Public URL: https://hdl.handle.net/2324/7324467

  • A fluorescent photochromic compound for labeling biomolecules Reviewed International journal

    N. Soh, K. Yoshida, H. Nakajima, K. Nakano, T. Imato, T. Fukaminato, M. Irie

    Chemical Communications   2007 ( 28 )   5206 - 5208   2007.12

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    A fluorescent photochromic compound, composed of diarylethene, fluorescein and succinimidyl ester units, was developed for the controllable fluorescent labeling of biomolecules based on a small molecule.

    DOI: DOI https://doi.org/10.1039/B713663C

    Repository Public URL: https://hdl.handle.net/2324/7324466

  • Swallow-tailed perylene derivative: a new tool for fluorescent imaging of lipid hydroperoxides Reviewed International journal

    N. Soh, T. Ariyoshi, T. Fukaminato, H. Nakajima, K. Nakano, T. Imato

    Organic Bimolecular Chemistry   5 ( 23 )   3762 - 3768   2007.12

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    A swallow-tailed perylene derivative including a triphenylphosphine moiety was synthesized and applied to the detection and the live-cell imaging of lipid hydroperoxides. The novel probe, named Spy-LHP, reacted rapidly and quantitatively with lipid hydroperoxides to form the corresponding oxide, Spy-LHPOx, which emits extremely strong fluorescence (Φ ∼ 1) in the visible range (λem = 535 nm, 574 nm). Spy-LHP was highly selective for lipid hydroperoxides, and the addition of other reactive oxygen species (ROS) including hydrogen peroxides, hydroxyl radical, superoxide anion, nitric oxide, peroxynitrite, and alkylperoxyl radical, caused no significant increase in the fluorescence intensity. The probe exhibited good localization to cellular membranes and was successfully applied to the confocal laser scanning microscopy (CLSM) imaging of lipid hydroperoxides in live J774A.1 cells, in which lipid peroxidation was proceeded by the stimulation of 2,2-azobis(2-amidinopropane)dihydrochloride (AAPH). These findings establish Spy-LHP as a promising new tool for investigating the physiology of lipid hydroperoxides.

    DOI: https://doi.org/10.1039/B713223A

    Repository Public URL: https://hdl.handle.net/2324/7324465

  • メチルパラチオン計測のための表面プラズモン共鳴イムノセンサーの開発 Reviewed

    田中真由美,阪本一平,中嶋 秀,宗 伸明,中野幸二,Duck-Hwa CHUNG,今任稔彦

    分析化学   56 ( 9 )   705 - 712   2007.9

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    表面プラズモン共鳴現象(SPR)を利用したオンサイトで簡便に残留農薬を計測できるフロー型イムノセンサーの開発を目的とし,メチルパラチオン(MP)の間接競合イムノアッセイ法を確立した.牛血清アルブミン(BSA)にMPを化学結合したMP-BSAコンジュゲートをSPRセンサーチップ上に物理吸着によって固定化し,これをフローシステムに組み込み,MPに対する応答性を検討した.SPRセンサーチップは,50 ppm MP-BSAコンジュゲート溶液をセンサーチップへ導入し,続いて10 mg/mL BSA溶液を導入し,非特異吸着を防ぐために未修飾のチップ表面をブロッキングして作製した.MP-BSAコンジュゲートのセンサーチップへの固定化密度を,飽和吸着量の約20% として作製したセンサーチップを用いて,間接競合法により60 ppm抗MP抗体を含むMP溶液(1~5000 ppb)をSPRセンサーに導入し,SPRセンサーの共鳴角度変化を測定した.本法によるMPの検出限界は,MP濃度ゼロにおける角度変化の85% におけるMP濃度とすると,10 ppbであった.また,ペプシンを含むpH 2 HCl-glycine溶液をセンサーチップに導入することによって,センサーチップ上のMP-BSAコンジュゲートと結合した抗MP抗体を解離させることができ,1枚のセンサーチップを用いて少なくとも20回繰り返し測定が可能であることが分かった.

    DOI: https://doi.org/10.2116/bunsekikagaku.56.705

    Repository Public URL: https://hdl.handle.net/2324/7324464

  • Sequential Injection Chemiluminescence Immunoassay for Nonionic Surfactants Using Magnetic Microbeads Reviewed International journal

    RuiQi Zhang, Hizuru Nakajima, Nobuaki Soh, Koji Nakano, Takashi Masadome, Kazumi Nagata, Kazuhira Sakamoto, Toshihiko Imato

    Analytica Chimica Acta   600 ( 1-2 )   105 - 113   2007.9

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    A rapid and sensitive immunoassay based on a sequential injection analysis (SIA) using magnetic microbeads for the determination of alkylphenol polyethoxylates (APnEOs) is described. An SIA system was constructed from a syringe pump, a switching valve, a flow-through type immunoreaction cell equipped with a photon counting unit and a neodymium magnet. Magnetic beads, to which an anti-APnEOs monoclonal antibody was immobilized, were used as a solid support in an immunoassay. The introduction, trapping and release of the magnetic beads in and from the immunoreaction cell were controlled by means of a neodymium magnet and adjusting the flow of a carrier solution. The immunoassay was based on an indirect competitive immunoreaction of an anti-APnEOs monoclonal antibody immobilized on the magnetic beads with a sample APnEOs and a horseradish peroxidase (HRP)-labeled APnEOs in the same sample solution, and was based on the subsequent chemiluminscence reaction of HRP on the magnetic microbeads with a luminol solution containing hydrogen peroxide and p-iodophenol. The anti-APnEOs antibody was immobilized on the magnetic microbeads by coupling the antibody with the magnetic beads after activation of a carboxylate moiety on the surface of the magnetic beads that had been coated with a polylactic acid film. The antibody immobilized magnetic beads were introduced in the immunoreaction cell and trapped in it by the neodymium magnet, which was equipped beneath the immunoreaction cell. An APnEOs sample solution containing the HRP-labeled APnEOs at a constant concentration, and a luminol solution containing hydrogen peroxide and p-iodophenol were sequentially introduced into the immunoreaction cell, according to an SIA programmed sequence. Chemiluminescence emission was monitored by means of a photon counting unit located at the upper side of the immunoreaction cell by collecting the emitted light with a lens. A typical sigmoidal calibration curve was obtained, when the logarithm of the concentration of APnEOs was plotted against the chemiluminescence intensity as the number of photons in 100 ms using standard APnEOs sample solutions at various concentrations (0-1000 ppb) under optimum conditions. The lower detection limit defined as IC(80) is ca 10 ppb. The time required for analysis is less than 15 min per a sample. The present method was successfully applied to the determination of APnEOs in river water.

    DOI: DOI: 10.1016/j.aca.2007.02.052

    Repository Public URL: https://hdl.handle.net/2324/7324463

  • Cytochrome c Self-Assembly on Alkanethiol Monolayer Electrodes as Characterized by AFM, IR, QCM, and Direct Electrochemistry Reviewed International journal

    Koji Nakano, Tadateru Yoshitake, Yasunori Yamashita, Edmond F. Bowden

    Langmuir   23 ( 11 )   6270 - 6275   2007.5

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    With the advantage of carbodiimide coupling chemistry, horse heart cytochrome c (cyt c) has been covalently immobilized onto self-assembled monolayers (SAMs) from 11-mercaptoundecanoic acid (MUDA) developed on single-crystal or polycrystalline gold substrate surfaces. The cyt c immobilized substrates thus prepared have been characterized by atomic force microscopy (AFM); we have succeeded in obtaining surface topographical images down to single-protein resolution. AFM imaging has also shown densely packed, uniform protein monolayer formation that is highly suggestive of self-assembly of cyt c molecules on MUDA SAMs. Covalent attachment of cyt c has been further evidenced by reflection−absorption FT-IR as well as microgravimetric analysis using a quartz crystal microbalance (QCM). In the latter, the specific MUDA and cyt c surface concentrations were determined to be 0.86 ± 0.11 nmol cm-2 (n = 5) and 28 ± 12 pmol cm-2 (n = 5), both of which agree fairly well with their theoretical counterparts. The obtained QCM chips having the cyt c/MUDA/Au interfacial structure were found to be capable of the direct electrochemistry of the surface-attached cyt c molecules. Cyclic voltammetric measurements on the chips gave particular redox waves showing characteristics of surface process. The electroactive protein surface concentration was determined to be 7.2 ± 4.8 pmol cm-2 (n = 6); it was almost consistent with values found in literature, while it was limited to 26% in magnitude for the QCM data. This was deemed to have arisen from the orientation variation of the surface-confined cyt c molecules and is discussed briefly.

    DOI: https://doi.org/10.1021/la063697w

    Repository Public URL: https://hdl.handle.net/2324/7324461

  • Surface Plasmon Resonance Immunosensor for IgE Analysis Using Two Types of Anti-IgE Antibodies with Different Active Recognition Sites Reviewed International journal

    Yan LI, Jujie REN, Hizuru NAKAJIMA, Nobuaki SOH, Koji NAKANO, Toshihiko IMATO

    Analytical Sciences   23 ( 1 )   31 - 38   2007.1

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    A simple and novel method for the determination of an IgE antibody based on a surface plasmon resonance immunosensor for the diagnosis of an allergy is described. The method involves the use of an anti-IgE(D) antibody and an anti-IgE(H) antibody, which reacts with the Ce2 domain and the Ce3 domain of the IgE antibody. The anti-IgE(D) antibody was immobilized on the gold surface of a sensor chip by physical adsorption. An IgE antibody sample was incubated by adding it to an anti-IgE(H) antibody solution to form an anti-IgE(H) immunocomplex through a reaction of the Ce3 domain of the IgE antibody. The incubated solution was introduced onto the sensor chip and the immunocomplex of the IgE-anti-IgE(H) then reacted with the anti-IgE(D) antibody immobilized on the sensor chip through the Ce2 domain of the IgE antibody part of the IgE-anti-IgE(H) immunocomplex. The detection limit of the present method for the determination of the IgE antibody was about 10 ppb. The affinity constants for the anti-IgE(H) antibody immunocomplex with the IgE antibody in solution and that of the anti-IgE(H) antibody immunocomplex with the IgE antibody immobilized on the sensor chip by a biotin-streptavidin interaction were estimated to be 4.1 × 107 M-1 and 5.8 × 106 M-1, respectively. The affinity constant for the immunocomplex of the anti-IgE(H) antibody with the IgE antibody with the anti-IgE(D) immobilized on the sensor chip was estimated to be 4.9 × 107 M-1, 20-times larger than the affinity constant for the IgE antibody immunocomplex with the anti-IgE(D) antibody immobilized on the sensor chip, based on a direct immunoassay method of the IgE antibody under the same experimental conditions.

    DOI: https://doi.org/10.2116/analsci.23.31

    Repository Public URL: https://hdl.handle.net/2324/7324459

  • Electrical Transport Through DNA-Ferrocene Conjugate as Characterized by Conductive AFM Measurement Invited

    Koji Nakano

    Proceedings of The 2nd International Symposium on Functional Innovation of Molecular Informatics   2006.11

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  • Poly(hydroquinone)-coated electrode for immobilizing of 5’-amine functioned capture probe DNA and electrochemical response to DNA hybridization Reviewed International journal

    K. Nakano, G. Hirayama, M. Toguchi, K. Nakamura, K. Iwamoto, N. Soh, T. Imato

    Science and Technology of Advanced Materials   7 ( 7 )   718 - 725   2006.10

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    Enzymatically polymerized hydroquinone, PHQ, was applied for polymer-coated electrode whose surface was further modified with 5′-amine dodecamer DNAs (capture probe DNA, CP-DNA) by taking advantage of Michael reaction. The film-forming property of PHQ on graphite substrate surfaces was confirmed to be satisfactory by AFM imaging. Cyclic voltammetric (CV) measurements showed that the PHQ-modified graphite electrode gave well-defined redox waves showing characteristics for surface process. The pH dependence of the formal potential, E1/2, suggested that the electrode reaction occurred by two-proton and two-electron mechanism (−59 mV per a pH decade). CVs also gave the specific amount of PHQ adsorption of 0.52 or 0.83 nmol cm−2 (monomer unit) for the different electrode preparations. This was indicative of two- or three-monolayer adsorption of PHQ. For applications of gene detection, the immobilization reaction including the CP-DNA hybridization was studied by microgravimetric analysis using a quartz-crystal microbalance (QCM). Summaries for the two different runs were 1.01 and 0.69 nmol cm−2 (monomer unit) for PHQ adsorption on gold surfaces, 0.19 and 0.15 nmol cm−2 for CP-DNA attachment on the PHQ/Au, and 0.14 and 0.11 nmol cm−2 for hybridization with the complementary DNA on the CP-DNA/PHQ/Au, respectively. Importantly, monitoring of the series of the experiments were possible by measuring the voltammetric properties of the electrode; the distinct redox waves due to PHQ-electrode reaction are suppressed upon immobilizing of the CP-DNA, and its hybridization further suppressed the redox activity. Neither treating of the PHQ-electrode with native DNAs nor treating of CP-DNA/PHQ-electrode with non-target DNA gave no noticeable responses. A possible mechanism for the electrode response was discussed briefly based on electrochemical QCM measurements. We think these observations are important as the basis of DNA hybridization sensor that enables totally, label-free electrochemical detection of the target DNA.

    DOI: https://doi.org/10.1016/j.stam.2006.06.007

  • Photoactive, covalent attachment of DNA on gold with double-strand specificity using self-assembled monolayers containing psoralen Reviewed International journal

    K. Nakano, H. Matsunaga, K. Sai, N. Soh, T. Imato

    Anal. Chim. Acta   578 ( 1 )   93 - 99   2006.9

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    Taking advantages of psoralen photochemistry, we have developed a new method of immobilizing DNA on gold substrate surfaces. A psoralen derivative having an alkylamine function was synthesized, and was self-assembled on gold substrate surfaces in a combined use of a thiol-derivatized molecule, 3,3′-dithiobis(succinimidyl propionate) forming amide bonds on the surface. We found that by irradiating with long wavelength ultraviolet light (320–400 nm), DNA molecules added in the solution phase were covalently immobilized on the monolayer surface through the photoadduct formation of the psoralen molecules with the DNA nucleobases. The present method has its advantage that is applicable to native DNAs, no chemically modifying DNAs, in spite of its covalent immobilization principle. We have examined 12 mer synthetic oligonucleotide immobilizations and have found that the surface concentration thus attained was to be 20 pmol cm−2, which is consistent with saturated surface coverage. Interestingly, the immobilization occurred double-stranded-DNA-preferentially; no immobilization for single-stranded DNAs. Characterization of the immobilization chemistry has been achieved using atomic force microscopic imaging, infrared absorption, X-ray photoelectron spectroscopy, electrochemistry, and quartz-crystal microbalance and their results were described.

    DOI: https://doi.org/10.1016/j.aca.2006.04.085

    Repository Public URL: https://hdl.handle.net/2324/7324458

  • Surface plasmon resonance immunosensor for histamine based on an indirect competitive immunoreaction Reviewed International journal

    Y. LI, M. KOBAYASHI, K. FURUI, N. SOH, K. NAKANO, T. IMATO

    Analytica Chimica Acta   576 ( 1 )   77 - 83   2006.8

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    he use of a surface plasmon resonance immunosensor for the analysis of histamine (β-imidazole ethylamine) is described. The method is based on an indirect competitive reaction of an anti-histamine antibody in a sample solution with histamine immobilized on a sensor chip and with histamine in the sample solution. A sensor chip immobilized with histamine was prepared using a self-assembly monolayer of 11-mercaptoundecanoic acid (11-MUA) as an anchor membrane, followed by an amino-coupling reaction with histamine after activation of the 11-MUA layer on the sensor chip by treatment with 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide and N-hydroxysuccinimide. The sensor chip can be reused, after regeneration with a 10 mM HCl solution, which dissociates the anti-histamine antibody complex from histamine on the sensor chip. The affinity constants for the immunocomplex of the anti-histamine antibody with histamine in the solution and for that of the anti-histamine antibody with histamine immobilized on the sensor chip were calculated to be 1.5 × 107 and 7.2 × 105 M−1, respectively, by assuming a Langmuir-type adsorption of the anti-histamine antibody to histamine immobilized on the sensor chip. The detection limit of the method was determined to be 3 ppb.

    DOI: https://doi.org/10.1016/j.aca.2006.01.078

    Repository Public URL: https://hdl.handle.net/2324/7324457

  • Novel fuorescent probe for detecting hydroperoxides with strong emission in the visible range Reviewed International journal

    Nobuaki Soh, Daisuke Seto, Koji Nakano, Toshihiko Imato

    Molecular Biosystems   16 ( 11 )   81 - 84   2006.6

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    A novel fluorescent probe, a swallow-tailed perylene derivative for detecting hydroperoxides (Spy-HP), containing perylene 3,4,9,10-tetracarboxyl bisimide as the main skeleton in the structure, was developed. Spy-HP reacted rapidly with hydroperoxides such as m-chloroperbenzoic acid (MCPBA) and cumene hydroperoxide to form its oxidized derivative, Spy-HPOx, and emitted an extremely strong fluorescence (Φ ∼ 1) in the visible range (λex = 524 nm and λem = 535 nm), as the result of canceling the photoinduced electron transfer (PET) effect. The reaction between Spy-HP and hydroperoxides proceeded quantitatively in strict stoichiometry, without being affected by autoxidation or photobleaching. Because of these prominent properties, Spy-HP is expected to be a novel and useful fluorescent probe to ‘spy’ on hydroperoxides in biosamples.

    DOI: https://doi.org/10.1016/j.bmcl.2006.02.078

    Repository Public URL: https://hdl.handle.net/2324/7324456

  • Electrochemical Sandwich Immunoassay for Vitellogenin by Sequential Injection Analysis Using Antibody Immobilized Magnetic Microbeads Reviewed International journal

    K. Hirakawa, M. Katayama, N. Soh, K. Nakano, H. Ohura, S. Yamasaki, T. Imato

    Electroanalysis   18 ( 13-14 )   1297 - 1305   2006.6

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    A rapid and sensitive sandwich immunoassay based on a sequential injection analysis (SIA) for the determination of carp vitellogenin (Vg) is described. The SIA system was constructed from a syringe pump, a multiposition valve, a flow-through type immunoreaction cell equipped with a magnet and an amperometric detector. Magnetic microbeads immobilized with an anti-Vg monoclonal antibody (primary antibody) were used as a solid support. The primary antibody was immobilized on magnetic microbeads by coupling the primary antibody with a polylactic acid-layer, which was coated on the surface of the magnetic beads, after activated with N-hydroxysuccinimide. The introduction, trapping and flushing out of the magnetic microbeads in the immunoreaction cell were controlled by the magnet and the flow of the carrier solution. After the primary antibody-immobilized magnetic beads were introduced and trapped in the immunoreaction cell, a Vg sample solution, an alkaline phosphatase (AP)-labeled anti-Vg polyclonal antibody (secondary antibody) solution and a p-aminophenyl phosphate (PAPP) solution were sequentially introduced into the immunoreaction cell based on an SIA programmed sequence. Vg was determined by the electrochemical detection of p-aminophenol (PAP), an enzymatic product of PAPP via the action by AP labeled on the secondary antibody. A solution containing PAP, which was generated in the immunoreaction cell and transiently held in a holding coil, was transported to the amperometric detector and the oxidation current of PAP on a working electrode applied at +0.20 V was measured. A sigmoidal calibration curve was obtained in the concentration range from 1 ppb to 500 ppb in a plot of oxidation current against the logarithm of the Vg concentration. The lower detection limit of the immunoassay was about 2–3 ppb. The time required for an analysis was ca. 15 min/sample.

    DOI: https://doi.org/10.1002/elan.200603529

    Repository Public URL: https://hdl.handle.net/2324/7324455

  • Methodology of reversible protein labeling for ratiometric fluorescent measurement Reviewed International journal

    N. SOH, D. SETO, K. NAKANO, T. IMATO

    Molecular BioSystems   2 ( 2 )   128 - 131   2006.2

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    The first fluorescent labeling technology, which can induce not only an increase in the fluorescence intensity but also a shift in the fluorescence spectrum, has been developed for “ratiometric” measurements for a protein by utilizing a newly designed “field-sensitive” fluorescent probe and its corresponding unique amino acid tag.

    DOI: DOI https://doi.org/10.1039/B515777C

    Repository Public URL: https://hdl.handle.net/2324/7324391

  • Electrochemical Immunoassay for Vitellogenin Based on Sequential Injection Using Antigen-immobilized Magnetic Microbeads Reviewed

    K. HIRAKAWA, M. KATAYAMA, N. SOH, K. NAKANO, T. IMATO

    Analytical Sciences   22 ( 1 )   81 - 86   2006.1

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    A rapid and sensitive immunoassay for the determination of vitellogenin (Vg) is described. The method involves a sequential injection analysis (SIA) system equipped with an amperometric detector and a neodymium magnet. Magnetic beads, onto which an antigen (Vg) was immobilized, were used as a solid support in an immunoassay. The introduction, trapping and release of magnetic beads in an immunoreaction cell were controlled by means of the neodymium magnet and by adjusting the flow of the carrier solution. The immunoassay was based on an indirect competitive immunoreaction of an alkaline phosphatase (ALP) labeled anti-Vg monoclonal antibody between the fraction of Vg immobilized on the magnetic beads and Vg in the sample solution. The immobilization of Vg on the beads involved coupling an amino group moiety of Vg with the magnetic beads after activation of a carboxylate moiety on the surface of magnetic beads that had been coated with a polylactate film. The Vg-immobilized magnetic beads were introduced and trapped in the immunoreaction cell equipped with the neodymium magnet; a Vg sample solution containing an ALP labeled anti-Vg antibody at a constant concentration and a p-aminophenyl phosphate (PAPP) solution were sequentially introduced into the immunoreaction cell. The product of the enzyme reaction of PAPP with ALP on the antibody, p-aminophenol, was transported to an amperometric detector, the applied voltage of which was set at +0.2 V vs. an Ag/AgCl reference electrode. A sigmoid calibration curve was obtained when the logarithm of the concentration of Vg was plotted against the peak current of the amperometric detector using various concentrations of standard Vg sample solutions (0 - 500 ppb). The time required for the analysis is less than 15 min.

    DOI: https://doi.org/10.2116/analsci.22.81

    Repository Public URL: https://hdl.handle.net/2324/7324451

  • Covalent attachment of DNA with double-strand specificity using self-assembled monolayers from psoralen International journal

    Koji NAKANO, Hideshi MATSUNAGA, Keisuke SAI

    Proceedings of The 2005 International Chemical Congress of Pacific Basin Societies   2005.12

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  • Preparation, Characterization and Atomic Force Microscopic Imaging of New DNA Ligand for Single Molecular Hybridization Assay International journal

    Hideshi MATSUNAGA, Koji NAKANO, Nobuaki SOH, Toshihiko IMATO

    Proceedings of The 2005 International Chemical Congress of Pacific Basin Societies   2005.12

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  • DNA Conjugate Electrode Having DNA/Oligo(1,4-hydroquinone)/PG Interfacial Structure for Biosensor Applications: Characterization by Electrochemical Measurements, QCM Analysis, and AFM Imaging International journal

    Koji NAKANO, Go HIRAYAMA, Mikiko TOGUCHI, Kaori NAKAMURA, Kaori IWAMOTO, Nobuaki SOH, Toshihiko IMATO

    Program & Abstracts for the 8th Asian Conference on Analytical Sciences   2005.10

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  • Sequential injection chemiluminescence immunoassay for anionic surfactants using magnetic microbeads immobilized with an antibody. Reviewed International journal

    R-Q. Zhang, K. Hirakawa, D. Seto, N. Soh, K. Nakano, T. Masadome, K. Nagata, K. Sakamoto, T. Imato

    Talanta   68 ( 2 )   231 - 238   2005.10

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    A rapid and sensitive immunoassay for the determination of linear alkylbenzene sulfonates (LAS) is described. The method involves a sequential injection analysis (SIA) system equipped with a chemiluminescence detector and a neodymium magnet. Magnetic beads, to which an anti-LAS monoclonal antibody was immobilized, were used as a solid support in an immunoassay. The introduction, trapping and release of the magnetic beads in the flow cell were controlled by means of a neodymium magnet and adjusting the flow of the carrier solution. The immunoassay was based on an indirect competitive immunoreaction of an anti-LAS monoclonal antibody on the magnetic beads and the LAS sample and horseradish peroxidase (HRP)-labeled LAS, and was based on the subsequent chemiluminscence reaction of HRP with hydrogen peroxide and p-iodophenol, in a luminol solution. The anti-LAS antibody was immobilized on the beads by coupling the antibody with the magnetic beads after activation of a carboxylate moiety on the surface of magnetic beads that had been coated with a polylactic acid film. The antibody immobilized magnetic beads were introduced, and trapped in the flow cell equipped with the neodymium magnet, an LAS solution containing HRP-labeled LAS at constant concentration and the luminol solution were sequentially introduced into the flow cell based on an SIA programmed sequence. Chemiluminescence emission was monitored by means of a photon counting unit located at the upper side of the flow cell by collecting the emitted light with a lens. A typical sigmoid calibration curve was obtained, when the logarithm of the concentration of LAS was plotted against the chemiluminescence intensity using various concentrations of standard LAS samples (0–500 ppb) under optimum conditions. The time required for analysis is less than 15 min.

    DOI: 10.1016/j.talanta.2005.07.012

    Repository Public URL: https://hdl.handle.net/2324/7172714

  • Synthesis of New Psoralen Derivatives for Chemical Modification and Functionalization of DNA Duplexes as Characterized by Gel Electrophoresis, IR, QCM, Electrochemistry, and AFM Reviewed

    T. TSURUTA, Y. KATSUMI, S. SHIRAKAWA, S. TAGUCHI, K. NAKANO

    Abstract Book of International Symposium on Nano-organization and Function   2004.11

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  • Covalent Attachment of Double-Stranded DNA using Self-Assembled Monolayers Containing Psoralen as Characterized by XPS, IR, QCM, Electrochemistry, and AFM Reviewed

    H. MATSUNGAE, K. SAI, K. NAKANO

    Abstract Book of International Symposium on Nano-organization and Function   2004.11

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  • Monolayer Formation of Glucose Oxidase Covalently Attached on ω-Aminoalkanethiol/Au via Activation of Carbohydrate Residue of GOx as Characterized by AFM, IR, QCM, and Biosensor Applications International journal

    K. NAKANO, K. DOI, K. TAMURA, Y. KATSUMI

    Abstract of The 7th Asian Conference on Analytical Sciences   2004.10

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  • Proposal of new modification technique for linear double-stranded DNAs using the polysaccharide schizopyllan Reviewed International journal

    T. Anada, H. Matsunaga, R. Karinaga, K. Koumoto, M. Mizu, K. Nakano, S. Shinkai, and K. Sakuraia

    Bioorganic & Medicinal Chemistry Letters   14 ( 22 )   5655 - 5659   2004.9

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    A natural polysaccharide schizophyllan (SPG) has been known to form a stable complex with poly(dA). We attached a poly(dA)(80) tail to the both ends of a linear double-stranded DNA, which had been prepared from a plasmid DNA vector. The poly(dA) tailed DNA verified to form complex with SPG by gel electrophoresis and atomic force microscopy (AFM). AFM images indicated that the complexes exhibit a dumbbell-like architecture, that is, quite similar to that of adenovirus genome. The complex demonstrated excellent exonuclease resistance, probably because of the protection effect by SPG complexation.

    DOI: 10.1016/j.bmcl.2004.08.043

  • Proposal of new modification technique for linear double-stranded DNAs using the polysaccharide schizopyllan Reviewed International journal

    T. Anada, H. Matsunaga, R. Karinaga, K. Koumoto, M. Mizu, K. Nakano, S. Shinkai, and K. Sakuraia

    Bioorganic & Medicinal Chemistry Letters   14 ( 22 )   5655 - 5659   2004.9

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    DOI: 10.1016/j.bmcl.2004.08.043

  • A psoralen Derivative Containing Ferrocene for Redox-labeling of DNA and Electrochemical Gene Sensor Applications Reviewed

    K. Nakano, S. Shirakawa, S. Taguchi

    Chemcial Sensors   2004.7

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  • DNA-Ferrocene Conjugate for Nanoelectronic Material Applications. Atomic Force Microscope-Based, Direct Measurement of Electrical Transport through DNA Molecules Invited

    Koji Nakano

    Second 21st Century COE “Towards Creating New Industries Based on Inter-Nanoscience” and 7th SANKEN International Symposium on “Hybridization of Chemistry, Biology, and Material Science”   2004.1

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  • Self-Assembling Monolayer Formation of Glucose Oxidase and Cytochrome c Covalently Attached on Alkanethiol Monolayers on Gold Reviewed International journal

    K. Nakano, K. Doi, K. Tamura, Y. Katsumi

    Biomolecular Chemistry. Proceedings of the ISBC 2003   2003.12

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  • Self-assembling Monolayer Formation of Glucose Oxidase Covalently Attached on 11-Aminoundecanethiol Monolayers on Gold Reviewed International journal

    K. NAKANO, K. DOI, K. TAMURA, Y. KATSUMI

    Chemical Communications   ( 13 )   1544 - 1545   2003.6

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    Glucose oxidase (GOx) has been attached covalently to form uniform enzyme monolayers on self-assembled monolayers (SAMs) from 11-aminoundecanethiol (AUDT) by taking advantage of chemical oxidation of GOx carbohydrate residues followed by coupling the resulting ‘aldehydic’ enzyme with the terminal amino group in the SAM as characterized by AFM imaging, IR, QCM, and electrochemical measurements.

    DOI: 10.1039/b303298a

    Repository Public URL: https://hdl.handle.net/2324/7324384

  • Dopamine as a polymerizable reagent for enzyme-linked immunosorbent assay using horseradish peroxidase Reviewed International journal

    @Sumed Yadoung, #Shinichi Shimizu, @Surat Hongsibsong, Koji Nakano, @Ryoichi Ishimatsu

    Heliyon   9 ( 11 )   e21722   2023.11

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    We demonstrate that dopamine can be used as a reagent for colorimetric enzyme-linked immunosorbent assay (ELISA) using horseradish peroxidase (HRP). Dopamine was able to be polymerized in the presence of HRP and H2O2, and black polydopamine was obtained after the enzymatic reaction. Because of the black color, the absorbance was significantly changed in the whole range of the visible light region. Here, an indirect competitive ELISA based on the polymerization of dopamine was performed to detect a fluoroquinolone antibiotic, enrofloxacin. The antibiotic is commonly used in livestock farming. The anti-antibiotics antibody was produced from egg yolk from chicken hens. In the visible range, sufficient absorbance changes of ~0.4~0.5 and a low background level for the ELISA response were obtained, and the 50 % inhibitory concentration value at 450 nm was determined to be 26 ppb. The performance of the indirect competitive ELISA based on the polymerization of dopamine was compared to that based on the
    oxidation of catechol because dopamine has a catechol skeleton. By the complex of HRP and H2O2, catechol can be oxidized to o-benzoquinone having a maximum absorption wavelength of 420 nm. It was shown that the absorbance change in the case of polydopamine was about 2.5 times higher than that of catechol, where the background levels were similar. This confirms that
    the polymerization of dopamine significantly enhanced the photosignal.

    DOI: https://doi.org/10.1016/j.heliyon.2023.e21722

  • Dopamine as a polymerizable reagent for enzyme-linked immunosorbent assay using horseradish peroxidase

    Yadoung, S; Shimizu, S; Hongsibsong, S; Nakano, K; Ishimatsu, R

    HELIYON   9 ( 11 )   e21722   2023.11   ISSN:24058440 eISSN:2405-8440

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    We demonstrate that dopamine can be used as a reagent for colorimetric enzyme-linked immunosorbent assay (ELISA) using horseradish peroxidase (HRP). Dopamine was able to be polymerized in the presence of HRP and H2O2, and black polydopamine was obtained after the enzymatic reaction. Because of the black color, the absorbance was significantly changed in the whole range of the visible light region. Here, an indirect competitive ELISA based on the polymerization of dopamine was performed to detect a fluoroquinolone antibiotic, enrofloxacin. The antibiotic is commonly used in livestock farming. The anti-antibiotics antibody was produced from egg yolk from chicken hens. In the visible range, sufficient absorbance changes of ∼0.4∼0.5 and a low background level for the ELISA response were obtained, and the 50 % inhibitory concentration value at 450 nm was determined to be 26 ppb. The performance of the indirect competitive ELISA based on the polymerization of dopamine was compared to that based on the oxidation of catechol because dopamine has a catechol skeleton. By the complex of HRP and H2O2, catechol can be oxidized to o-benzoquinone having a maximum absorption wavelength of 420 nm. It was shown that the absorbance change in the case of polydopamine was about 2.5 times higher than that of catechol, where the background levels were similar. This confirms that the polymerization of dopamine significantly enhanced the photosignal.

    DOI: 10.1016/j.heliyon.2023.e21722

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  • Monomer and Excimer Emission in Electrogenerated Chemiluminescence of Pyrene and 2,7-Di-<i>tert</i>-butylpyrene Associated with Electron Transfer Distance

    Ishimatsu, R; Tashiro, S; Nakano, K

    JOURNAL OF PHYSICAL CHEMISTRY B   127 ( 43 )   9346 - 9355   2023.10   ISSN:1520-6106 eISSN:1520-5207

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    Electrogenerated chemiluminescence (ECL) is a light emission phenomenon caused by electrochemically generated radical anions (R•-) and cations (R•+), in which the ion annihilation results in the formation of a pair of excited (R*) and ground state (R) of a luminescent molecule. Here, the ECL properties of pyrene (Py) and 2,7-di-tert-butylpyrene (di-t-BuPy) are reported. It was found that at a commonly employed concentration (1 mM), the ECL spectra were time-dependent because of increasing the oligomer emission and increasing the concentration of R near R*, leading to an enhancement of the excimer emission. At a low concentration range (20-30 μM), the shape of the ECL spectra containing the monomer and excimer emission was determined by isolated pairs of R* and R, which were generated through ion annihilation of R•- and R•+. It was found that in the ECL of Py and di-t-BuPy originated from the isolated pairs of R•- and R•+, 58 and 48% of the excited states were the excimer states, respectively. Diffusion equation analysis indicates that the lower excimer formation in the case of di-t-BuPy is because of a farther initial separation distance between R* and R, i.e., a longer electron transfer distance between the radical ions. The Marcus model for the electron transfer kinetics suggests that the farther electron transfer distance is mainly caused by the larger molecular size, which resulted in a smaller reorganization energy of the solvent acetonitrile molecule. Taking advantage of the photophysical and electrochemical properties of Py and di-t-Bu Py, the monomer and excimer emission in ECL is discussed.

    DOI: 10.1021/acs.jpcb.3c05602

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  • Monomer and Excimer Emission in Electrogenerated Chemiluminescence of Pyrene and 2,7-Di-tert-butylpyrene Associated with Electron Transfer Distance Reviewed International journal

    @Ryoichi Ishimatsu, #Shuya Tashiro, Koji Nakano

    Journal of Physical Chemistry B   127 ( 43 )   9346 - 9355   2023.10

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    Electrogenerated chemiluminescence (ECL) is a light emission 6 phenomenon caused by electrochemically generated radical anions (R•−) and 7 cations (R•+), in which the ion annihilation results in the formation of a pair of 8 excited (R*) and ground state (R) of a luminescent molecule. Here, the ECL 9 properties of pyrene (Py) and 2,7-di-tert-butylpyrene (di-t-BuPy) are reported. It was found that at a commonly employed concentration (1 mM), the ECL spectra were time-dependent because of increasing the oligomer emission and increasing the concentration of R near R*, leading to an enhancement of the excimer emission. At a low concentration range (20−30 μM), the shape of the ECL spectra containing the monomer and excimer emission was determined by isolated pairs of R* and R, which were generated through ion annihilation of R•− and R•+. It was found that in the ECL of Py and di-t-BuPy originated from the isolated pairs of R•− and R•+, 58 and 48% of the excited states were the excimer states, respectively. Diffusion equation analysis indicates that the lower excimer formation in the case of di-t-BuPy is because of a farther initial separation distance between R* and R, i.e., a longer electron transfer distance between the radical ions. The Marcus model for the electron transfer kinetics suggests that the farther electron transfer distance is mainly caused by the larger molecular size, which resulted in a smaller reorganization energy of the solvent acetonitrile molecule. Taking advantage of the photophysical and electrochemical properties of Py and di-t-Bu Py, the monomer and excimer emission in ECL is discussed.

    DOI: https://doi.org/10.1021/acs.jpcb.3c05602

  • Development of a facile time-resolved spectroelectrochemical method: An application to determine the rate constant of protonation for anions of 9,10-diphenylanthracene, biphenyl, and p-quaterphenyl Reviewed International journal

    Ryoichi Ishimatsu, #Yoshie Furukawa, Koji Nakano,

    Journal of Electroanalytical Chemistry   921   116684 - 116684   2022.9   ISSN:1572-6657 eISSN:1873-2569

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    Using a conventional three-electrode system and electrochemical cell, we developed a simple spectroelectro-
    chemical method that can be used to determine the rate constant of protonation reaction for several reduced
    species. A glassy carbon (GC) disk electrode as a working electrode and halogen lamp as a light source were
    employed. The reflected light on the GC electrode surface through the diffusion layer of electrochemically gen-
    erated species was recorded by an electron multiplying charge coupled device camera equipped with a spec-
    trometer. With this system, the absorbance change by the generation of the radical anion of 9,10-
    diphenylanthracene (DPA), biphenyl, and p-quaterphenyl was monitored. The molar extinction coefficient
    (ε) of the radical ions was calculated with the absorbance and charge during potential steps. The absorption
    spectrum of electrochemically generated dianion of p-quaterphenyl was also monitored, and the ε value was
    determined. The comproportionation reaction between p-quaterphenyl2− and neutral p-quaterphenyl was also
    clearly observed on the spectroelectrochemical data. The absorbance change during the protonation at differ-
    ent concentration of ethanol (cEtOH) for DPA•−, biphenyl•−, p-quaterphenyl•−, and p-quaterphenyl2− and the ε
    values were used to determine the first-order rate constant of the protonation reaction. The first-order rate con- 2 •− •− 2− 3
    stant increased linearly against cEtOH for DPA , p-quaterphenyl , and p-quaterphenyl , and against cEtOH for biphenyl•−. From the slopes, the reaction rate was determined to be 0.45, 4.2×102, and 4.3×104 M−2 s−1, for DPA•−, p-quaterphenyl•−, and p-quaterphenyl2−, respectively, and for biphenyl•−, 6.8×104 M−3 s−1.

    DOI: 10.1016/j.jelechem.2022.116684

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    Repository Public URL: https://hdl.handle.net/2324/7236547

  • Direct Electron Transfer Reaction of Cytochrome c Immobilized on a Bare ITO Electrode Reviewed

    @Naoki Matsuda, @Hirotaka Okabe, @Toshihiko Nagamura, Koji Nakano

    Bulletin of the Chemical Society of Japan   94 ( 2 )   433 - 439   2021.1

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    To measure the direct electron transfer (DET) reaction of cytochrome c (Cytc) immobilized on a bare ITO electrode after removing the adsorbed molecules, automated solution exchange (ASE) processes were performed to induce their desorption. By fitting the absorbance decay curve observed at the Soret band peak position of Cytc at around 408 nm during the ASE processes with a double exponential equation, the final immobilized fraction was estimated to be 58.6% of the Cytc adsorbed on bare ITO electrodes under the experimental conditions. Cyclic voltammograms (CVs) of Cytc adsorbed on the bare ITO electrodes were measured for 60 min to elucidate the DET activity of immobilized Cytc. After repeated CV measurements, approximately 90% of immobilized Cytc was found to remain from the evaluation based on the coulombic amount of reduction and oxidation peaks. The scan rate dependent peak separation data from the immobilized Cytc between reduction and oxidation peaks in CVs produced 2.7 times larger DET reaction rate constant than that previously reported for the Cytc adsorbed on the bare ITO electrode.

    DOI: https://doi.org/10.1246/bcsj.20200192

    Repository Public URL: https://hdl.handle.net/2324/7236794

  • Enzyme-linked immunosorbent assay based on light absorption of enzymatically generated aniline oligomer: Flow injection analysis for 3-phenoxybenzoic acid with anti-3-phenoxybenzoic acid monoclonal antibody Reviewed International journal

    R. Ishimatsu, #S. Shimizu, @S. Hongsibsong, K. Nakano, C. Malasuk, Y. Oki, @K. Morita

    Talanta   218   121102   2020.10

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    DOI: https://doi.org/10.1016/j.talanta.2020.121102

    Repository Public URL: https://hdl.handle.net/2324/7173486

  • Kinetics of Excimer Electrogenerated Chemiluminescence of Pyrene and 1-Pyrenebutyricacid 2-Ethylhexylester in Acetonitrile and an Ionic Liquid, Triethylpentylphosphonium Bis(trifluoromethanesulfonyl)imide Reviewed

    Ryoichi Ishimatsu, Shuya Tashiro, Takashi Kasahara, Juro Oshima, Jun Mizuno, Koji Nakano, Chihaya Adachi, Toshihiko Imato

    Journal of Physical Chemistry B   123 ( 50 )   10825 - 10836   2019.12

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    We describe the kinetics of excimer electrogenerated chemiluminescence (ECL) of a liquid pyrene derivative, 1-pyrenebutyricacid 2-ethylhexylester (PLQ) dissolved in a molecular solvent, acetonitrile (MeCN), and an ionic liquid, triethylpentylphosphonium bis(trifluoromethanesulfonyl)imide ([P2225][TFSI]). Pyrene was also used for comparison. To discuss the kinetics of the excimer ECLs, the photophysical and electrochemical properties and electronic states of PLQ and pyrene were revealed. The photoluminescence (PL) spectra, rate constants for the radiative transitions, and redox potentials of PLQ and pyrene dissolved in MeCN and [P2225][TFSI] suggest that as a solvent, [P2225][TFSI] behaves more polar than MeCN. By analyzing the PL decay curves, the rate constants to form the excimer were determined to be on the order of 109 and 107 M-1 s-1 in MeCN and [P2225][TFSI], respectively, which were limited by the diffusion. For neat PLQ (1.6 M), a delay of 0.3-0.4 ns for the excimer emission compared to the monomer emission was observed. It is likely that the delay corresponds to the timescale for arranging the conformation to form the excimer. The ECL of PLQ was generated by applying a square wave voltage to produce the radical anion and cation, and on the ECL spectra, the excimer emission was more prevailed compared to the PL spectra. Kinetic analysis for the electron transfer reaction between the radical ions based on Marcus theory indicates that the electron transfer is limited by the diffusion of the radical ions. Moreover, the electron transfer distance (det) between the radical cation and anion to generate excited states was calculated with a framework of the theory. Kinetically, the electron transfer can take place at det < 11 Å in MeCN and det < 12 Å in [P2225][TFSI]. The density functional theory (DFT) and time-dependent DFT calculations show that the potential energy curve of the excimer against the distance between the pyrene rings reaches a minimum at 3.50 Å. This suggests that through the electron transfer, the process of the direct formation of the monomer S1 state followed by the excimer formation is more prevailed than that of the direct excimer formation.

    DOI: 10.1021/acs.jpcb.9b08813

    Repository Public URL: https://hdl.handle.net/2324/7237089

  • Efficient Electrogenerated Chemiluminescence of Pyrrolopyrrole Aza-BODIPYs in the Near-Infrared Region with Tripropylamine Involving Formation of S2 and T2 States Reviewed

    Ryoichi Ishimatsu, Hirosato Shintaku, Yuto Kage, Misaki Kamioka, Soji Shimizu, Koji Nakano, Hiroyuki Furuta, Toshihiko Imato

    Journal of the American Chemical Society   141 ( 30 )   11791 - 11795   2019.7

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    Efficient electrogenerated chemiluminescences (ECLs) of three pyrrolopyrrole aza-BODIPYs in the near-infrared region by using tripropylamine as a coreactant are reported. Kinetic analysis based on Marcus theory indicates the direct formation of S2 and T2 states through the electron transfer reaction, which affects the ECL efficiencies.

    DOI: 10.1021/jacs.9b05245

  • Electrogenerated Chemiluminescence of Tris(dibenzoylmethane)phenanthroline Europium(III) as a Light Source: an Application for the Detection of PO43– Based on the Ion Associate Formation of Phosphomolybdic Acid and Malachite Green Reviewed International journal

    #Eri KUNISAWA, Ryoichi ISHIMATSU, Koji NAKANO, and Toshihiko IMATO†

    Chemistry Select   35 ( 7 )   799 - 802   2019.6

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    We demonstrate that electorogenerated chemiluminescence (ECL) of an organometallic Eu(III) complex, tris(dibenzoylmethane)phenanthroline europium(III), whose emission spectra is very sharp with the maximum wavelength of 612 nm, can be used as a light source for the detection of PO43– based on changing the absorbance of the ion associate of malachite green (MG+) and phosphomolybdic acid. The ECL was also applied to measure the absorbance of MG+. With the detection system we established, absorbance up to ∼1.6 with a change of ∼0.1 could be detected.

    DOI: https://doi.org/10.2116/analsci.19N003

    Repository Public URL: https://hdl.handle.net/2324/7237097

  • Portable Analytical Detection Systems Based on Light Emitting Devices Reviewed

    Ryoichi Ishimatsu, Eri Kunisawa, Hirosato Shintaku, Koji Nakano, Toshihiko Imato

    2019 International Conference on Electronics Packaging, ICEP 2019   294 - 297   2019.4

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    Portable analytical detection systems based on light emitting devices, organic light-emitting diode (OLED), and electrogenerated chemiluminescence (ECL) are described. The OLED is composed of an Eu(III) complex, whose emission spectrum is very sharp, was applied for fluorescence detection, and ECL of a boron-dipyrromethene derivative showing relatively sharp spectrum was used for the absorbance measurements.

    DOI: 10.23919/ICEP.2019.8733418

  • Electrogenerated Chemiluminescence and Electronic States of Several Organometallic Eu(III) and Tb(III) Complexes:Effectsof the Ligands Reviewed International journal

    Ryoichi Ishimatsu, #Eri Kunisawa, Koji Nakano, Chihaya Aachi, Toshihiko Imato

    Chemistry Select   4 ( 9 )   2815 - 2831   2019.3

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    DOI: DOI: 10.1002/slct.201900595

    Repository Public URL: https://hdl.handle.net/2324/7237099

  • Electrogenerated Chemiluminescence of a BODIPY Derivative with Extended Conjugation Reviewed International journal

    Ryoichi Ishimatsu, #Hirosato Shintaku, Chihaya Aachi, Koji Nakano, Toshihiko Imato

    Chemistry Select   2 ( 32 )   10531 - 10536   2017.11

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    DOI: DOI: 10.1002/slct.201702449

    Repository Public URL: https://hdl.handle.net/2324/7238743

  • Determination of the Solvent Polarity of Ionic Liquids Based on Stokes Shifts of a Donor-Acceptor Molecule

    石松 亮一, 田代 修也, 中野 幸二, 今任 稔彦

    九州大学中央分析センター報告(印刷中)   2017.3

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  • Quenching Behavior of Thermally Activated Delayed Fluorescence from a Donor-Acceptor Molecule, 1,2,3,5-Tetrakis(carbazol-9-yl)-4,6- dicyanobenzene by O2 Reviewed International journal

    Ryoichi Ishimatsu, Yuko Kirino, Chihaya Aachi, Koji Nakano, Toshihiko Imato

    Chemistry Letters   45 ( 10 )   1183 - 1185   2016.10

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    DOI: DOI: http://dx.doi.org/10.1246/cl.160561

    Repository Public URL: https://hdl.handle.net/2324/7238788

  • Development of Surface Plasmon Resonance Sensor Chip for Multiplex Sample Detection Reviewed International journal

    Jie Zhan, Ryoichi Ishimatsu, Koji Nakano, Toshihiko Imato

    J. Flow Injection Analysis   33 ( 1 )   23 - 32   2016.6

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    A method for preparation of a surface plasmon resonance (SPR) sensor chip with 8-stripe pattern for multiplex immunoassay, where several kinds of antibodies can be immobilized, is proposed in this paper. For immobilization of two types of antibody, anti-IgA and anti-IgG antibodies on the sensor chip at 8-stripe pattern alternatively, a flow generated by the centrifugal force was used for introducing the antibody solutions separately into a microchip with 8-channel on the 8-stripe patterned sensor chip. The resulting sensor chip, on which the anti-IgA antibody and anti-IgG antibody were immobilized alternatively, was attached to a flow cell prepared from an acrylic with a single flow channel. Mixed solutions of IgA and IgG at different concentrations were injected into a carrier stream of a pH buffer solution at 7.4, and SPR sensor signals for IgA and IgG were detected separately from the SPR sensor chip with stripes immobilized with corresponding antibodies. As a result, calibration curves for IgA and IgG, which fit to those calculated from the Langmuir adsorption isotherm, were obtained. The detection limit of the SPR sensor was ca. 5 ppm.

    DOI: https://doi.org/10.24688/jfia.33.1_23

    Repository Public URL: https://hdl.handle.net/2324/7325096

  • Development of a Portable Surface Plasmon Resonance Sensor with Multi-Sensing Points based on the Linear CCD Sensor Reviewed International journal

    Jie Zhan, Koji Furui, Hizuru Nakajima, Noriaki Kaneki, Ryoichi Ishimatsu, Koji Nakano, Toshihiko Imato

    Analytical Sciences   32 ( 6 )   673 - 679   2016.6

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    DOI: DOI: 10.2116/analsci.32.673

    Repository Public URL: https://hdl.handle.net/2324/7238801

  • Photophysical Properties and Efficient Stable Electrogenerated Chemiluminescence of Donor-Acceptor Molecules Exhibiting Thermal Spin Up-Conversion Reviewed International journal

    Ryoichi Ishimatsu, Tomohiko Ezura, Chihaya Aachi, Koji Nakano, Toshihiko Imato

    Chemistry a European Journal   22 ( 3 )   4889 - 4898   2016.3

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    DOI: DOI:10.1002/chem.201600077

    Repository Public URL: https://hdl.handle.net/2324/7238809

  • Flow-Injection Spectrophotometric Determination of Cysteine in Biologically Active Dietary Supplements Reviewed International journal

    Anastasiia PETROVA, Ryoichi Ishimatsu, Koji Nakano, Toshihiko Imato, Andrey VISHNIKIN, Leonid MOSKVIN, Andrey BULATOV

    Journal of Analytical Chemistry   71 ( 2 )   172 - 178   2016.2

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    A procedure is developed for the flow-injection spectrophotometric determination of cysteine in dietary supplements based on the formation of a reduced phosphomolybdic complex by the analyte in mixing a sample solution with a solution of ammonium 18-molybdophosphate. For the flow-injection determination of cysteine, a polymethyl methacrylate chip is fabricated, in the channels of which the analytical form was obtained and detected. The detection limit for cysteine is 3 × 10–6 M and the throughput is 240 determinations per hour. The procedure was tested in the analysis of different samples of dietary supplements; the results obtained were verified by HPLC.

    DOI: DOI: 10.1134/S1061934816020118

  • A Miniaturized Stepwise Injection Spectrophotometric Analyzer Reviewed International journal

    Anastasiia PETROVA, Andrey BULATOV, Andrey VISHNIKIN, Leonid MOSKVIN, Ryoichi Ishimatsu, Koji Nakano, Toshihiko Imato

    Analytical Sciences   31 ( 6 )   529 - 533   2015.6

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    DOI: http://doi.org/10.2116/analsci.31.529

    Repository Public URL: https://hdl.handle.net/2324/7325075

  • An Electrochemical Compact Disk-type Microfluidics Platform for Use as an Enzymatic Biosensor Reviewed International journal

    Poomrat Rattanarat, Prinjaporn Teengam, Weena Siangproh, Ryoichi Ishimatsu, Koji Nakano, Orawon Chailapakul, Toshihiko Imato

    Electroanalysis   27   703 - 712   2015.3

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    DOI: http://dx.doi.org/10.1002/elan.201400590

    Repository Public URL: https://hdl.handle.net/2324/7325074

  • Fluorometric flow-immunoassay for alkylphenol polyethoxylates on a microchip containing a fluorescence detector comprised of an organic light emitting diode and an organic photodiode Reviewed International journal

    Rong Liu, Ryoichi Ishimatsu, Masayuki Yahiro, Chihaya Aachi, Koji Nakano, Toshihiko Imato

    Talanta   134   37 - 47   2015.3

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    DOI: http://dx.doi.org/10.1016/j.talanta.2014.10.055

    Repository Public URL: https://hdl.handle.net/2324/7173485

  • Automated Chemiluminescence Immunoassay for a Nonionic Surfactant using a Recycled Spinning-pausing Controlled Washing Procedure on a Compact Disc-type Microfluidic Platform Reviewed International journal

    Sui Guo, Ryoichi Ishimatsu, Koji Nakano, Toshihiko Imato

    Talanta   133   100 - 106   2015.2

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    DOI: http://dx.doi.org/10.1016/j.talanta.2014.06.075

    Repository Public URL: https://hdl.handle.net/2324/7173483

  • Photometric Flow Injection Determination of Phosphate on a PDMS Microchip Using an Optical Detection System Assembled with an Organic Light Emitting Diode and an Organic Photodiode Reviewed International journal

    Rong Liu, Ryoichi Ishimatsu, Masayuki Yahiro, Chihaya Aachi, Koji Nakano, Toshihiko Imato

    Talanta   132   96 - 105   2015.1

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    DOI: http://dx.doi.org/10.1016/j.talanta.2014.08.057

    Repository Public URL: https://hdl.handle.net/2324/7173484

  • 表面プラズモン共鳴センサを利用した 免疫グロブリンGのフローインジェクション分析 Reviewed International journal

    Ryoichi Ishimatsu, Ruey-Feng Chang, Yuko Kirino, Koji Nakano, Toshihiko Imato

    J. Flow Injection Analysis   31 ( 2 )   111 - 114   2014.12

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    Direct detection of immunoglobulin G (IgG) by using a surface plasmon resonance (SPR) sensor through an antigen‒antibody interaction was described. An anti‒IgG antibody was chemically immobilized on a sensor chip through a self‒assembled monolayer (SAM) in order to enhance the recognition of an IgG antigen. By assuming the Langmuir adsorption isotherm, maximum amount of adsorbed anti‒IgG antibody on the sensor chip and an apparent biding constant of the IgG antigen to anti‒IgG antibody were estimated. The amount of the IgG antigen bound to the anti‒IgG antibody, which was immobilized onto the sensor chip through the SAM was three times higher compared with the amount of the IgG antigen bound to the anti‒IgG antibody, which was immobilized directly to the sensor chip through physical adsorption.

    DOI: https://doi.org/10.24688/jfia.31.2_111

    Repository Public URL: https://hdl.handle.net/2324/7325095

  • Electrogenerated Chemiluminescence of Donor-Acceptor Molecules Displaying Thermally Activated Delayed Fluorescence Reviewed International journal

    Ryoichi Ishimatsu, Shigeyuki Matsunami, Takashi Kasahara, Jun Mizuno, Tomohiko Ezura, Chihaya Aachi, Koji Nakano, Toshihiko Imato

    Angewandte Chemie International Edition   53   6933 - 6996   2014.7

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    DOI: http://dx.doi.org/10.1002/anie.201402615

    Repository Public URL: https://hdl.handle.net/2324/7325073

  • An Organic Thin Film Photodiode as a Portable Photodetector for the Detection of Alkylphenol Polyethoxylates by a Flow Fluorescence-Immunoassay on Magnetic Microbeads in a Microchannel Reviewed International journal

    Ryoichi Ishimatsu, Azusa Naruse, Rong Liu, Koji Nakano, Masayuki Yahiro, Chihaya Aachi, Toshihiko Imato

    Talanta   117   139 - 145   2013.12

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    DOI: http://dx.doi.org/10.1016/j.talanta.2013.08.044

    Repository Public URL: https://hdl.handle.net/2324/7173454

  • Solvent Effect on Thermally Activated Delayed Fluorescence by 1,2,3,5-Tetrakis(carbazol-9-yl)-4,6-dicyanobenzene Reviewed International journal

    Ryoichi Ishimatsu, Shigeyuki Matsunami, Katsuyuki Shizu, Chihaya Aachi, Koji Nakano, Toshihiko Imato

    The Journal of Physical Chemistry A   117 ( 27 )   5607 - 5612   2013.6

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    DOI: http://dx.doi.org/10.1021/jp404120s

    Repository Public URL: https://hdl.handle.net/2324/7325021

  • Chemiluminescence immunoassay for a nonionic surfactant using a compact disc-type microfluidic platform Invited Reviewed International journal

    Shuai Guo, Koji Nakano, Hizuru Nakajima, Katsumi Uchiyama, Akihide Hammi, Yoshikazu Yamasaki, Shigeharu Morooka, Ishimatsu Ryoichi, Toshihiko Imato

    Pure and Applied Chemistry   84 ( 10 )   2027 - 2043   2012.10

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    A simple and pump-free chemiluminescence immunoassay based on a compactdisc (CD)-type microfluidic platform for the determination of alkylphenol polyethoxylates(APnEOs) is described. The method is based on a competitive immunoreaction of the anti-APnEOs antibody immobilized on the magnetic microbeads between APnEOs and horse-radish peroxidase (HRP)-labeled APnEOs in the sample solution. The luminol solutioncontaining H2O2and enhancer is caused to flow from one reservoir in the platform to anotherby appropriate adjustment of the speed of the rotation of the disc. The detection limit wassimilar to that reported in our previous paper, i.e., 10 ppb according to IC80.

    DOI: https://doi.org/10.1351/PAC-CON-11-10-10

    Repository Public URL: https://hdl.handle.net/2324/7325015

  • Performance of an organic photodiode as an optical detector and its application to fluorometric flow-immunoassay for IgA Reviewed International journal

    M. Miyake, Hizuru Nakajima, A. Henmi, M. Yahiro, CHIHAYA ADACHI, Nobuaki Soh, Ishimatsu Ryoichi, Koji Nakano, K. Uchiyama, Toshihiko Imato

    Talanta   96   132 - 139   2012.7

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    The performance of an organic thin film photodiode (OPD), fabricated from a hetero-junction comprised of two layers of C60 and a phthalocyanine-Cu(II) complex was evaluated by detecting the chemilumi- nescence generated from the reaction of luminol with horseradish peroxidase in the presence of H2O2, and the fluorescence from resorufin, as an optical detector. The photocurrent of the OPD was linear with respect to the power of light from a commercial LED. The sensitivity of the OPD was sufficient for detecting chemiluminescence with a power 0.1 W/cm2 . The OPD was successfully used in a flow-immunoassay for IgA, a marker of human stress, in which a sandwich immunoassay was carried out on the microchip and the fluorescence from resorufin, produced by the enzymatic reaction, was detected. The detection limits for resorufin and IgA were 5.0 M and 16 ng/mL, respectively. The photosensitivity of the OPD remained relatively constant for a minimum of one year.

    DOI: doi:10.1016/j.talanta.2012.02.006

    Repository Public URL: https://hdl.handle.net/2324/7172721

  • A Simple and Selective Fluorometric Assay for Dopamine using a Calcein Blue-Fe2+ Complex Fluorophore Reviewed International journal

    D. Seto, T. Maki, Nobuaki Soh, Koji Nakano, Ishimatsu Ryoichi, Toshihiko Imato

    Talanta   94   36 - 43   2012.5

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    A surface plasmon resonance (SPR) sensor on a compact disk (CD)-type microfluidic device was developed to miniaturize the elements of a complete analytical system, pump and valves. The CD-type microfluidic device was fabricated by attaching a poly- dimethylsiloxane disk plate that contained microchannels and reservoirs to a flat poly- carbonate disk plate that contained grating films with a thin layer of Au. The optical system of the SPR sensor and the theory for its operation are based on the principle of a grating coupled-type SPR. The sample and reagent solutions in the reservoirs on the CD- type microfluidic device were sequentially introduced into the detection chamber by centrifugal force generated by the rotation of the microfluidic device. The variation of resonance wavelength was dependent on the refractive index of the sample solution. This CD-type SPR sensor was successfully used in an immunoassay of immunoglobulin A (IgA). The anti-IgA, blocking reagent, sample and washing solution in the reservoirs were sequentially introduced into the detection chamber by changing the frequency of rotation of the microfluidic device. IgA in the sample solution was adsorbed to the anti-IgA immobilized on the Au thin layer in the detection chamber and was then detected by the SPR sensor.

    DOI: doi:10.1016/j.talanta.2012.02.025

    Repository Public URL: https://hdl.handle.net/2324/7172724

  • An integrated enzyme-linked immunosorbent assay system with an organic light-emitting diode and a chargecoupled device for fluorescence detection Reviewed International journal

    HIZURU Nakajima, Yukiko Okuma, Kazuhiro Morioka, Mayo Miyake, Akihide Hemmi, Tatsuya Tobita, Masayuki Yahiro, Daisuke Yokoyama, Chihaya Adachi, Nobuaki Soh, Koji Nakano, Shuhua Xue, Hulie Zeng, Katsumi Uchiyama, Toshihiko Imato

    J. Separation Science   34 ( 20 )   2906 - 2912   2011.10

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    A fluorescence detection system for a microfluidic device using an organic light-emitting diode (OLED) as the excitation light source and a charge-coupled device (CCD) as the photo detector was developed. The OLED was fabricated on a glass plate by photo- lithography and a vacuum deposition technique. The OLED produced a green lumines- cence with a peak emission at 512 nm and a half bandwidth of 55 nm. The maximum external quantum efficiency of the OLED was 7.2%. The emission intensity of the OLED at 10 mA/cm2 was 13 mW (1.7 mW/cm2). The fluorescence detection system consisted of the OLED device, two band-pass filters, a five microchannel poly(dimethylsiloxane) (PDMS) microfluidic device and a linear CCD. The fluorescence detection system was successfully used in a flow-based enzyme-linked immunosorbent assay on a PDMS microfluidic device for the rapid determination of immunoglobulin A (IgA), a marker for human stress. The detection limit (S/N 5 3) for IgA was 16.5 ng/mL, and the sensitivity was sufficient for evaluating stress. Compared with the conventional 96-well microtiter plate assay, the analysis time and the amounts of reagent and sample solutions could all be reduced.

    DOI: DOI 10.1002/jssc.201100429

    Repository Public URL: https://hdl.handle.net/2324/7324552

  • A surface plasmon resonance sensor on a compact disk-type microfluidic device Reviewed International journal

    Akihide Hemmi, Takashi Usui. Akihiro Moto, Tatsuya Tobita, Nobuaki Soh, Koji Nakano, Hulie Zeng, Katsumi Uchiyama, Toshihiko Imato, Hizuru Nakajima

    J. Separation Science   34 ( 20 )   2913 - 2919   2011.10

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    DOI: https://doi.org/10.1002/jssc.201100446

    Repository Public URL: https://hdl.handle.net/2324/7324553

  • Flow injection fluorometric determination of ascorbic acid using perylenebisimide-linked nitroxide International journal

    TOMOHARU MAKI, NOBUAKI SOH, Koji NAKANO, TOSHIHIKO IMATO

    Talanta   85 ( 4 )   2011.9

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    A simple and sensitive flow injection fluorometric method for the determination of ascorbic acid is described. Perylenebisimide-linked nitroxide (PBILN) is used as a fluorescent reagent, which permits the selective determination of ascorbic acid. The fluorescence of the perylenebisimide moiety in PBILN is quenched by the nitroxide moiety, which is linked to the perylenebisimide. When a stream of a solution of ascorbic acid is merged with a stream of PBILN, the ascorbic acid reacts with the nitroxide moiety of PBILN to form hydroxylamine, and the fluorescence properties of the perylenebisimide moiety are recovered. As a result, a peak-shaped fluorescence signal is produced, which can be observed by a fluo- rescence detector located downstream. Under optimized conditions, a good linear relationship between the concentration of ascorbic acid and peak height in the concentration range from 0.5 to 10 mol L−1 was found and the detection limit (S/N = 3) was 0.28 mol L−1 . The relative standard deviation for the determination of 4.0 mol L−1 ascorbic acid samples was 1.0% (n = 5). The proposed method was applied to the determination of ascorbic acid in several soft drink beverages and the analytical results were in good agreement with those obtained using a conventional method.

    DOI: doi:10.1016/j.talanta.2011.06.054

    Repository Public URL: https://hdl.handle.net/2324/25712

  • Anodic Stripping Voltammetry Combined with Sequential Injection Analysis for Measurements of Trace Metal Ions with Bismuth- and Antimony Film Electrodes under Comparable Conditions Reviewed International journal

    V. GUZSVANY, H. NAKAJIMA, N. SOH, K. NAKANO, I. SVANCARA, K. VYTRAS, L. BJELICA, T. IMATO

    Electroanalysis   23 ( 7 )   1539 - 1601   2011.7

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    Anodic stripping voltammetry combined with sequential injection analysis (ASV-SIA) was selected to examine the use of bismuth- and antimony-film plated glassy carbon electrodes under comparable conditions for the determination of Pb(II) and Cd(II) ions. Of interest were the conditions for film deposition, as well as the composition of sample/carrier solutions, including concentrations of Sb(III) or Bi(III) and HCl. Then, by the optimized procedure, one could determine Pb(II), Cd(II), and Zn(II) ions at the low µg L−1 level and ASV-SIA configuration with both electrodes tested on analysis of a water sample.

    DOI: https://doi.org/10.1002/elan.201000683

    Repository Public URL: https://hdl.handle.net/2324/7324493

  • Structure-Reactivity Relationship of Piperidine Nitroxide: Electrochemical, ESR and Computational Studies International journal

    T. YAMASAKI, F. MITO, Y. ITO, S. PANDIAN, Y. KINOSHITA, K. NAKANO, R. MURUGESAN, K. SAKAI, H. UTSUMI, K. YAMASHITA

    J. Organic Chemistry   76 ( 2 )   435 - 440   2011.1

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    We have synthesized several nitroxides with different substituents which vary the steric and electronic environment around the N-O moiety and have systematically investigated the role of substituents on the stability of the radicals. Our results demonstrated the reactivity toward ascorbate correlates with the redox potential of the derivatives. Furthermore, ab initio calculations also indicated a correlation between the reduction rate and the computed singly occupied molecular orbital-lowest unoccupied molecular orbital energy gap, but not with solvent accessible surface area of the N-O moiety, supporting the experimental results and suggesting that the electronic factors largely determine the radicals’ stability. Hence, it is possible to perform virtual screening of nitroxides to optimize their stability, which can help to rationally design novel nitroxides for their potential use in vivo.

    DOI: DOI: 10.1021/jo101961m

    Repository Public URL: https://hdl.handle.net/2324/7324491

  • An Amphiphilic Fluorescent Probe for the Visualization of Histamine in Living Cells Reviewed International journal

    Daisuke Seto, Nobuaki Soh, Koji Nakano, Toshihiko Imato

    Bioorg. Med. Chem. Lett.   20   6708 - 6711   2010.11

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    A novel fluorescent probe composed of two moieties, Nile Red and an iminodiacetic acid–Ni2+ complex,
    for the detection of histamine in living cells is described. The probe was successfully applied to visualizing histamine in RAW264 cells, representing the first demonstration of the imaging of histamine itself in
    living cells.

    DOI: doi:10.1016/j.bmcl.2010.09.003

    Repository Public URL: https://hdl.handle.net/2324/7324490

  • Stimuli-responsive nanoparticles composed of naturally occurring amphiphilic proteins Reviewed International journal

    Noriho Kamiya, Yoshiaki Shiotari, Masamichi Tokunaga, Hideshi Matsunaga, Hirokazu Yamanouchi, Koji Nakano, Masahiro Goto

    Chem. Commun.   2009 ( 35 )   5287 - 5289   2010.9

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    Simple chemical cross-linking of β-casein micelles resulted in the formation of thermally responsive proteinaceous nanoparticles that exhibit lower critical solution temperatures.

    DOI: DOI https://doi.org/10.1039/B909897F

    Repository Public URL: https://hdl.handle.net/2324/7324485

  • Selective Fluorescence Detection of Histamine Based on Ligand Exchange Mechanism and Its Application to Biomonitoring Reviewed International journal

    Daisuke Seto, Nobuaki Soh, Koji Nakano, Toshihiko Imato

    Anal. Biochem.   404 ( 2 )   135 - 139   2010.9

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    We report on a novel histamine monitoring method by using a fluorescent probe, a complex between Ni2+ and calcein, based on a ligand exchange mechanism. The fluorescence intensity of this probe, which has been reduced due to effective quenching by Ni2+ ion, increases drastically by an addition of histamine. Furthermore, the probe shows high selectivity toward histamine among the various neurotransmitters in 0.1 M phosphate buffer solution (pH 7.4). Biomonitoring studies to detect histamine released from RAW264 cells are successfully represented.

    DOI: https://doi.org/10.1016/j.ab.2010.04.038

    Repository Public URL: https://hdl.handle.net/2324/7324489

  • Antimony-Film Electrode for the Determination of Trace Metals by Sequential-Injection Analysis/Anodic Stripping Voltammetry Reviewed International journal

    Valéria GUZSVANY, Hizuru NAKAJIMA, Nobuaki SOH, Koji NAKANO, Toshihiko IMATO

    Anal. Chim. Acta   658 ( 12-17 )   12 - 17   2010.1

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    The possibility of applying antimony-film modified glassy carbon electrode in sequential-injection analysis (SIA) was investigated with the objective of determining Pb(II) and Cd(II) by anodic stripping voltammetry (ASV). The conditions of antimony-film deposition concerning composition of the plating/carrier solutions, concentrations of Sb(III) and hydrochloric acid, effects of different supporting electrolyte salts, and plating potential were optimized. It was found that the antimony-film deposition on glassy carbon substrate in a sample solution consisting of 750 μg L−1 Sb(III), 0.5 mol L−1 HCl at −1.5 V (vs. Ag/AgCl/3 mol L−1 KCl) yielded a modified electrode suitable for the determination of Pb(II) and Cd(II) at the μg L−1 level. The reproducibility of the analytical signals was characterized by a relative standard deviation lower than 2.8%, and the calculated values of detection limits were 1.2 μg L−1 for Pb(II) and 1.4 μg L−1 for Cd(II). The presence of KSCN in the sample solution offers the possibility of detecting ions with more negative oxidation potentials like Zn(II), Mn(II) or Cr(III). The developed SIA-ASV procedure was compared with the commonly used batch method, and its applicability was tested on a spiked tap water sample.

    DOI: https://doi.org/10.1016/j.aca.2009.10.049

    Repository Public URL: https://hdl.handle.net/2324/7324487

  • Flow Immunoassay for Nonioinic Surfactants Based on Surface Plasmon Resonance Sensors Reviewed International journal

    Mayumi TANAKA, Kazuhira SAKAMOTO, Hizuru NAKAJIMA, Nobuaki SOH, Koji NAKANO, Takashi MASADOME, Toshihiko IMATO

    Anal.Sci.   25 ( 8 )   999 - 1005   2009.8

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    A rapid, sensitive immunoassay based on a surface plasmon resonance sensor in a flow system for the determination of alkylphenol polyethoxylate (APEO) is described. The method is based on an indirect competitive reaction between an anti-APEO antibody in the sample solution and APEO immobilized on a sensor chip and APEO in the same sample solution. A sensor chip was prepared by immobilizing an APEO-horseradish peroxidase (APEO-HRP) conjugate on the thin gold film of the sensor chip. The adsorption constants for the APEO-HRP conjugate on the sensor chip and the surface density of the APEO-HRP adsorbed on the sensor chip at the saturated state were estimated to be 4.7 × 105 M−1 and 5.0 × 10−14 mol/mm2, respectively, using a Langmuir adsorption isotherm equation and results from the adsorption experiments. The affinity constants for the immunocomplexes of the anti-APEO antibody with the APEO conjugate on the sensor chip and for APEO in the sample solution were estimated to 2.0 × 106 and 5.1 × 106 M−1, respectively. A typical sigmoid calibration curve for APEO was obtained in the concentration range from 1 ppb to 1000 ppb. The detection limit, defined as the concentration of APEO, at which 85% of the sensor signal was observed, was ca. 10 ppb. The assay was applied to the determination of APEO in tap water in conjunction with a solid phase extraction pretreatment; APEO levels of approximately 50 ppt were successfully determined.

    DOI: https://doi.org/10.2116/analsci.25.999

    Repository Public URL: https://hdl.handle.net/2324/7324483

  • Perylenebisimide-linked nitroxide for the detection of hydroxyl radicals Reviewed International journal

    Tomoharu Maki, Tuyoshi Fukaminato, Hizuru Nakajima, Koji Nakano, Toshihiko Imato

    Anal. Chim. Acta   639 ( 1-2 )   78 - 82   2009.4

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    A new type of fluorophore-linked nitroxide for the detection of hydroxyl radical (radical dotOH) was synthesized using perylene-3,4,9,10-tetracarboxyl bisimide and 2,2,6,6-tetramethylpiperidine-1-oxyl (TEMPO). This new fluorescent probe, indicated here as Spy-OH (swallow-tailed perylene derivative for detecting radical dotOH) has its natural fluorescence effectively reduced due to the quenching caused by the presence of the nitroxide radical in its structure. In the presence of radical dotOH, in media containing DMSO, a conspicuous increase in the fluorescence intensity was observed. No significant change in the fluorescence intensity was observed upon the addition of other several reactive oxygen species (ROS) except for radical dotOH. Spy-OH maxima excitation (λexc) and emission (λem) wavelengths lie in the visible region (520/530 nm), therefore, this fluorescent probe is expected to be useful for a simple and selective detection of radical dotOH, especially for biosamples.

    DOI: https://doi.org/10.1016/j.aca.2009.02.044

    Repository Public URL: https://hdl.handle.net/2324/7324479

  • Sequential Injection Flow Immunoassay Based on Surface Plasmon Resonance Sensors Reviewed International journal

    M. Tanaka, Y. Li, H. Nakajima, N. Soh, K. Nakano, K. Sakamot, T. Imato

    J. Flow Injection Analysis   25 ( 2 )   172 - 182   2008.12

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    Flow immunoassay methods for the determination of several environmental pollutants, such as 2,4-dichlorophenol, bisphenol A, trinitrophenol, pesticides and allergy related compounds, such as histamine and immunoglobulin E (IgE) based on a surface plasmon resonance (SPR) sensor are reviewed from the view point of methodology of immunoassay. In this review, two types of immobilization methods for an antibody or an antigen conjugate for the target molecule on the sensor chip are proposed. One is the immobilization method for the antibody of the target molecule, where the antibody is immobilized on the sensor chip through a gold binding polypeptide and Protein G by taking into account of high orientation of the antibody for an immunoreaction on the sensor chip. The other method is the immobilization of the antigen conjugate in two way, where an antigen conjugate with protein such as bovine serum albumin is immobilized by physical adsorption and an antigen conjugate is immobilized by amino-coupling with an alkane thiol compound, which is preliminary immobilized on the sensor chip as a self-assembled monolayer. In order to enhance the sensitivity of the SPR sensor by increasing the change in the mass on the sensor chip after the immunoreaction, a competitive immunoreactions is adopted for the both immobilization methods, where the antibody or the antigen conjugate is immobilized on the sensor chip. A sandwich immunoassay method for the allergy related compound, IgE, was demonstrated as a sensitive method for enhancement of the SPR sensor signal. In the present flow immunoassay, a sequential injection technique is utilized in order to automate the immunoassay protocol, which consists of many procedures.

    DOI: https://doi.org/10.24688/jfia.25.2_172

    Repository Public URL: https://hdl.handle.net/2324/7325097

  • Flow sandwich immunoassay for specific anti-OVA IgG antibody by use of surface plasmon resonance sensor Reviewed International journal

    Y. Li, J. Ren, H. Nakajima, B.-K. Kim, N. Soh, K. Nakano, T. Imato

    Talanta   77 ( 2 )   423 - 478   2008.12

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    A simple and sensitive method for the determination of a specific IgG antibody against ovalbumin (anti-OVA IgG antibody) for diagnosis of egg allergy by use of a surface plasmon resonance (SPR) immunosensor in a flow system is described. An OVA conjugate was immobilized on a sensor chip via a self-assembled monolayer of 11-mercaptoundecanoic acid through an amino coupling method. The determination of the anti-OVA IgG antibody was based on a kind of sandwich immunoassay using an anti-IgG secondary antibody in order to enhance the sensitivity of the SPR immunosensor. The sensitivity and detection limit of the present method for the anti-OVA IgG antibody were 7 mdeg/ppm and 300 ppb, respectively. The present method showed an enhanced sensitivity and detection limit for the determination of the anti-OVA antibody, compared with a direct immunoassay, by which the sensitivity and detection limit were 3 mdeg/ppm and 1 ppm, respectively. By assuming a Langmuir type of adsorption isotherm, the affinity constants of an anti-OVA IgG antibody immunocomplex with the anti-IgG secondary antibody and of the anti-OVA antibody to the OVA conjugate immobilized on the sensor chip were calculated to be 2.1 × 106 M−1 and 2.0 × 106 M−1, respectively. The present flow immunoassay for the anti-OVA IgG antibody by an SPR sensor has potential applications in both research and diagnosis of egg allergy.

    DOI: https://doi.org/10.1016/j.talanta.2008.03.007

    Repository Public URL: https://hdl.handle.net/2324/7172718

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Books

  • クリスチャンExcelで解く分析化学(角田欣一、戸田 敬監訳)

    中野 幸二(Role:Joint translator)

    丸善  2017.3 

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    Responsible for pages:6 その他の平衡反応及び滴定   Language:Japanese   Book type:Scholarly book

  • クリスチャン分析化学原書7版 I基礎編(今任稔彦、角田欣一監訳)

    中野 幸二(Role:Joint translator)

    丸善  2016.12 

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    Responsible for pages:14酸化還元滴定と電位差滴定、15ボルタンメトリーと電気化学センサー   Language:Japanese   Book type:Scholarly book

    第14章 酸化還元滴定と電位差滴定 :還元剤や酸化剤を使って試料を滴定する容量分析法がいろいろな定量分析に役立っている.滴定は目視で判別することができる指示薬を使うか,あるいは適当な指示電極で電位を測定して滴定曲線を得ることで行う.本章では,はじめに半反応式の電位に基づいて酸化還元滴定曲線を考察し,それに続いて,代表的な酸化還元滴定法、及び実験に際して適切な酸化状態の試料成分を得るための手順を説明する.電位差滴定曲線の作成については,微分滴定曲線やGranプロットへの拡張を含めて説明する.容量関係を利用して定量するためには,正しい化学量論で示された反応式が必要なので,酸化還元反応の収支をとることをはじめにおさらいしよう. 第15章 ボルタンメトリーと電気化学センサー:電気分解(電解法)を利用することで,もっとも感度が高い方法だけでなく,もっとも正確な機器分析法のいくつかが実現されている.これらの方法では,試料を電解反応用のセルに入れ,所定の電圧を電極に印加して試料を酸化,あるいは還元する(12章).このときの電解反応に必要な電気量(クーロン量か電流のいずれか)を,分析成分の量と関連づける.ボルタンメトリーでは試料の電流-電圧特性を調べるが,このとき電解される分析成分の割合はおそらくごく少なくて,実際には無視できる程度である.それでも,ボルタンメトリーではマイクロモルかそれ以下の濃度が測定できる.分析しようとする成分が酸化または還元される電位は,それぞれの物質によって決まっているので,電解反応の電位を適切に設定すれば特定の物質だけを反応させることができる.このような反応の選択性が備わっているので,多くの場合,前段分離は必要でない.そのため迅速な分析法になり得る.

  • Scanning Electrochemical Microscopy Imaging of DNA Arrays for High Throughput Analysis Applications, in Modern Aspects of Electrochemistry. Applications of Electrochemistry and Nanotechnology in Biology and Medicine, Ed. by N. Eliaz.

    Koji Nakano(Role:Joint author)

    Springer  2011.12 

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    Responsible for pages:105-142   Language:English   Book type:Scholarly book

  • 高木 誠編著,ベーシック分析化学

    中野幸二,高木 誠(Role:Joint author)

    化学同人  2006.9 

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    Responsible for pages:第5章 酸化還元反応   Language:Japanese   Book type:Scholarly book

  • 新海征治編著,モレキュラーインフォマティクスを拓く分子機能材料

    中野幸二(Role:Joint author)

    日刊工業出版  2006.3 

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    Responsible for pages:6.4 DNAコンジュゲートの電気化学DNAセンサー・マイクロアレイ応用   Language:Japanese   Book type:Scholarly book

  • Determination of Trace Metals by Sequential Injection-Anodic Stripping Voltammetry by Bismuth Film Electrode, in "Sensing in Electroanalysis"

    Valéria GUZSVANY, Hizuru NAKAJIMA, Nobuaki SOH, Koji NAKANO, Toshihiko IMATO(Role:Joint author)

    Pardubice, Czech Republic  2009.12 

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    Language:English   Book type:Scholarly book

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Presentations

  • ピロール-イミダゾールポリアミドの機能性色素コンジュゲート形成によるDNA二重らせんの蛍光/リン光分析試薬の研究

    #花山祐介,中野幸二,@石松亮一

    第83回分析化学討論会  2023.5 

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    Event date: 2023.5

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:富山市   Country:Japan  

  • Speciality peptides for sub-macromolecular probe for chemical analysis: D/A-modified pyrrole-imidazole polyamide for photon upconversion DNA analysis International conference

    #Keisuke Hanayama, Ryoichi Ishimatsu, Koji NAKANO

    The 3rd Kyushu-Mainz Chemistry Symposium & The 7th CMS International Symposium (CMS-7)  2022.12 

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    Event date: 2022.12

    Language:English   Presentation type:Oral presentation (general)  

    Venue:Hawaii (Online)   Country:United States  

  • 遺伝子のアップコンバージョン蛍光分析のためのドナー/アクセプター修飾ピロール-イミダゾールポリアミドの研究

    #花山 佳祐, 中野 幸二, 石松 亮一

    日本分析化学会第71回年会  2022.9 

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    Event date: 2022.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:岡山   Country:Japan  

  • PNAzyme: Monolithic peptide nucleic acid peptide sequence hybrid functioning as molecular recognition catalyst element for nucleic acids analysis International conference

    Koji Nakano, #Junich Tanabe, #Yusuke Sezaki, #Ryosuke Kobayashi, Ryoichi Ishimatsu

    2021 International Chemical Congress of Pacific Basin Societies  2021.12 

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    Event date: 2021.12

    Language:English   Presentation type:Oral presentation (general)  

    Venue:Hawaii (Online)   Country:United States  

  • ペプチド核酸/タンパク質酵素ハイブリット分子-PNAzymeの合成とバイオ分析への応用

    #小林 諒祐, 中野 幸二, 石松 亮一

    日本分析化学会第70回年会  2021.9 

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    Event date: 2021.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:神戸(オンライン)   Country:Japan  

  • マイクロペルオキシダーゼ/バニロイドレセプターハイブリッド15量体ペプチド酵素の化学合成とペルオキシダーゼ反応の基礎検討

    中野 幸二, #木下 美里, #姫野 俊基, 石松 亮一

    第81回分析化学討論会  2021.5 

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    Event date: 2021.5

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:米沢市(オンライン)   Country:Japan  

  • ペルオキシダーゼ活性を持つペプチド核酸モノリスハイブリッドーPNAzymeの合成と遺伝子センシング応用

    中野 幸二, #田邊 潤壱, 石松 亮一

    日本分析化学会第69年会  2020.9 

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    Event date: 2020.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:名古屋(オンライン)   Country:Japan  

  • バニロイドレセプターペプチドのメトキシクマリン修飾アラニン組込みおよびポリエチレングリコール化による新規ペプチドホスト分子の合成とバイオ分析応用

    中野 幸二, #堀内 潤, 石松 亮一

    日本分析化学会第68年会  2019.9 

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    Event date: 2019.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:千葉市   Country:Japan  

  • ペプチド核酸とタンパク質酵素のワンポット合成化合物-PNAzymeの研究と核酸分析への応用

    #瀬崎 佑介, 中野 幸二, 石松 亮一

    日本分析化学会第68年会  2019.9 

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    Event date: 2019.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:千葉市   Country:Japan  

  • 人工バニロイドレセプターペプチドのHelix-Turn-Helixフォールディングを伴う自己組織化膜形成とカプサイシンセンシング

    中野 幸二, #堀内 潤, #平田 真吾, #姫野 俊基, 石松 亮一

    第79回分析化学討論会  2019.5 

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    Event date: 2019.5

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:北九州市   Country:Japan  

  • PNAzyme-ペプチド核酸とタンパク質触媒のワンポット固相合成による新規核酸分析試薬の研究

    #瀬崎 佑介, #田邊 潤壱, 石松 亮一, 中野 幸二

    日本分析化学会第67年会  2018.9 

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    Event date: 2018.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:仙台市   Country:Japan  

  • バニロイド受容性電位チャネルタンパク質の分子認識ドメインの固相ペプチド合成とカプサイシンのバイオセンシング

    中野 幸二, #堀内 潤, #姫野 俊基, 石松 亮一, 今任 稔彦

    第78回分析化学討論会  2018.5 

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    Event date: 2018.5

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:宇部市   Country:Japan  

  • カーボン量子ドットの過シュウ酸エステル化学発光反応における蛍光体としての組み込みと過酸化水素分析への応用

    中野 幸二, #本田 敬之, #山﨑 香菜子, #田仲 慶紀, #谷口 渓一, #勝尾 祐太, 石松 亮一, 今任 稔彦

    日本分析化学会第67年会  2017.9 

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    Event date: 2017.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:仙台市   Country:Japan  

  • バニリン結合ドメインを組み込んだマイクロペルオキシダーゼ11の合成とバイオ分析への応用

    #姫野 俊基, 中野 幸二, 石松 亮一, 今任 稔彦

    日本分析化学会第66年会  2017.9 

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    Event date: 2017.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:東京都   Country:Japan  

  • 酸化還元タグ修飾デオキシリボヌクレオシドポリリン酸の合成とその電気化学ゲノム分析への応用

    #勝尾 祐太, #田邊 潤壱、#平田 龍太郎, 中野 幸二, 石松 亮一, 今任 稔彦

    日本分析化学会第66年会  2017.9 

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    Event date: 2017.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:東京都   Country:Japan  

  • マイクロペルオキシダーゼ11とその軸配位子改変体の化学合成と酵素機能評価

    中野 幸二、#平田 龍太郎、#田邊 潤壱、石松 亮一、今任 稔彦

    日本分析化学会第77回分析化学討論会  2017.5 

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    Event date: 2017.5

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:京都市   Country:Japan  

  • Total chemical synthesis of microperoxidase 11 and its variants for bioanalytical and bioelectrochemical purposes International conference

    Koji Nakano, #Junichi Tanabe, #Ryutaro Hirata, Ryoichi Ishimatsu, Toshihiko Imato

    International Congress on Alanytical Sciences (ICAS2017)  2017.5 

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    Event date: 2017.5

    Language:English   Presentation type:Oral presentation (general)  

    Venue:Hainan   Country:China  

  • Research of surface-functionalization using FMOC protection-deprotection of N-doped carbon quantum dots and application for fluorescence analysis International conference

    Kanako Yamasaki, Yoshiyuki Honda, Koji Nakano, Ryoichi Ishimatsu, Toshihiko Imato

    The 13th Asian Conference on Alanytical Sciences (ASIANALYSIS XIII)  2016.12 

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    Event date: 2016.12

    Language:English   Presentation type:Oral presentation (general)  

    Venue:Chiang Mai   Country:Thailand  

  • Design and synthesis of artificial mimics of transient receptor potential vanilloid type 1 channel protein for chemical pain sensing International conference

    Koji Nakano, Jun Horiuchi, Shingo Hirata, Ryoichi Ishimatsu, Toshihiko Imato

    The 13th Asian Conference on Alanytical Sciences (ASIANALYSIS XIII)  2016.12 

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    Event date: 2016.12

    Language:English   Presentation type:Oral presentation (general)  

    Venue:Chiang Mai   Country:Thailand  

  • Synthesis of new pyrrole-imidazole polyamide as base-sequence selective minor-groove binder for application to fluorescent DNA probe International conference

    Yoshiki Tanaka, Koji Nakano, Ryoichi Ishimatsu, Toshihiko Imato

    The 13th Asian Conference on Alanytical Sciences (ASIANALYSIS XIII)  2016.12 

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    Event date: 2016.12

    Language:English   Presentation type:Oral presentation (general)  

    Venue:Chiang Mai   Country:Thailand  

  • 窒素ドープカーボン量子ドットのFmoc保護-脱保護を利用する表面修飾法の研究と蛍光分析への応用

    山﨑 香奈子, 本田 敬之, 中野 幸二, 石松 亮一, 今任 稔彦

    日本分析化学会第65年会  2016.9 

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    Event date: 2016.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:札幌市   Country:Japan  

  • 蛍光性ピロール-イミダゾールポリアミドコンジュゲートの合成と塩基配列認識能を持つ二重らせん副溝結合性DNAプローブへの応用

    田仲 慶紀, 中野 幸二, 石松 亮一, 今任 稔彦

    日本分析化学会第65年会  2016.9 

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    Event date: 2016.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:札幌市   Country:Japan  

  • バニロイド受容性一過性受容器電位チャネルタンパク質(TRPV1)の分子認識ドメインペプチドの化学合成・ハイブリッド化によるバイオセンサー応用

    中野 幸二, 堀内 潤, 石松 亮一, 今任 稔彦

    日本分析化学会第76回分析化学討論会  2016.5 

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    Event date: 2016.5

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:岐阜市   Country:Japan  

  • Synthesis of functional hybrid proteins consist of substrate-binging domain in transient receptor potential vanilloid type 1 for biosensing applications International conference

    Jun Horiuchi, Koji Nakano, Shingo Hirata, Ryoichi Ishimatsu, Toshihiko Imato

    The 28th International Symposium on Chemical Engineering (ISChE 2015)  2015.12 

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    Event date: 2015.12

    Language:English   Presentation type:Oral presentation (general)  

    Venue:Jeju   Country:Korea, Republic of  

  • 材料結合性ペプチド−カーボン量子ドットコンジュゲートの化学合成と蛍光バイオセンシングへの応用

    本田 敬之, 上土井 宏太, 中野 幸二, 石松 亮一, 今任 稔彦

    日本分析化学会第64年会  2015.9 

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    Event date: 2015.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:福岡市   Country:Japan  

  • Hisタグ融合TRPV1ゲスト結合モチーフペプチドからの自己組織化単分子膜形成と匂い物質センシング

    堀内 潤, 平田 真吾, 中野 幸二, 石松 亮一, 今任 稔彦

    日本分析化学会第64年会  2015.9 

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    Event date: 2015.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:福岡市   Country:Japan  

  • フェロセン化デオキシヌクレオシドポリリン酸の合成とこれを基質に用いるDNA鋳型反応の電気化学分析

    平田 龍太郎, 田邊 潤壱, 中野 幸二, 石松 亮一, 今任 稔彦

    日本分析化学会第64年会  2015.9 

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    Event date: 2015.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:福岡市   Country:Japan  

  • 光架橋性分岐DNA二重らせんの自己組織化とデンドリマー形成および化学修飾電極の基礎特性

    森田 孝平, 澤田 貴文, 中野 幸二, 石松 亮一, 今任 稔彦

    日本分析化学会第64年会  2015.9 

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    Event date: 2015.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:福岡市   Country:Japan  

  • 人工マイクロペルオキシダーゼ11の化学合成と酵素機能評価

    田邊潤壱, 中野 幸二, 石松 亮一, 今任 稔彦

    第75回分析化学討論会  2015.5 

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    Event date: 2015.5

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:甲府市   Country:Japan  

  • Hisタグ融合TRPV1ゲスト結合モチーフペプチドからの自己組織化単分子膜形成とカプサイシンセンシング

    堀内 潤, 中野 幸二, 平田 真吾, 石松 亮一, 今任 稔彦

    日本分析化学会第63年会  2014.9 

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    Event date: 2014.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:広島市   Country:Japan  

  • フェロセン化デオキシヌクレオシドポリリン酸の合成とDNAを鋳型とする酵素反応の電気化学分析への応用

    田邊 潤壱, 中野 幸二, 平田 龍太郎, 石松 亮一, 今任 稔彦

    日本分析化学会第63年会  2014.9 

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    Event date: 2014.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:広島市   Country:Japan  

  • Self-Assembly of Vanilloid-Binging Domain in Transient Receptor Potential Cation Channels based on His-Tag Chemistry at Gold Surfaces and Guest-Molecule Responses International conference

    Koji Nakano, Shingo Hirata, Jun Horiuchi, Ryoichi Ishimatsu, Toshihiko Imato, Takeshi Onodera, Kenshi Hayashi

    The 15th IUMRS-International Conference in Asia (IUMRS-ICA 2014)  2014.8 

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    Event date: 2014.8

    Language:English   Presentation type:Oral presentation (general)  

    Venue:福岡市   Country:Japan  

  • 光架橋性分岐 DNA 二重らせんの自己組織化を利用するデンドリマー修飾電極の作製とその電気化学特性・バイオセンシング応用

    中野 幸二, 澤田貴文, 森 美詞, 山中 真, 石松 亮一, 今任 稔彦

    日本分析化学会第62年会  2013.9 

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    Event date: 2013.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:東大阪市   Country:Japan  

  • Synthesis of His-Tag Fused Vanilloid Receptor in Transient Receptor Potential Channels for Biosensing Application of Vanilloids International conference

    Shingo Hirata, Koji Nakano, Ryoichi Ishimatsu, Toshihiko Imato, Kenshi Hayashi

    The Twelfth Asian Conference on Analytical Sciences (ASIANALYSIS XII)  2013.8 

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    Event date: 2013.8

    Language:English   Presentation type:Oral presentation (general)  

    Venue:福岡市   Country:Japan  

  • Hisタグ融合TRPV1ゲスト結合モチーフペプチドからの自己組織化単分子膜形成とバイオセンシング応用

    中野 幸二, 平田真吾, 石松 亮一, 今任 稔彦

    第73回分析化学討論会  2013.5 

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    Event date: 2013.5

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:函館市   Country:Japan  

  • 光架橋性オリゴヌクレオチドからのDNAデンドリマー自己組織化膜形成~単分子膜の化学構造と電気化学特性~

    中野 幸二, 澤田 貴文, 森 美詞, 石松 亮一, 今任 稔彦

    電子情報通信学会有機エレクトロニクス研究会(OME)  2013.3 

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    Event date: 2013.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  • TRPV1のゲスト結合モチーフの化学合成と小分子ケトン類のバイオセンシングを目的としたHis-tag結合型自己組織化単分子膜の作成

    平田 真吾, 中野 幸二, 石松 亮一, 今任 稔彦, 林 健司

    日本分析化学会第61年会  2012.9 

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    Event date: 2012.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:金沢市   Country:Japan  

  • 光架橋反応と組み合わせた分岐DNA二重らせんからのデンドリマー形成とナノ粒子凝集を利用したターゲットDNA検出

    山中 真, 中野 幸二, 石松 亮一, 今任 稔彦

    日本分析化学会第61年会  2012.9 

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    Event date: 2012.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:金沢市   Country:Japan  

  • 原子間力顕微鏡イメージングと光結合性小分子を組み合わせたDNAインターカレーションに伴う二重らせんの巻き戻し・伸長現象の可視化解析

    中野 幸二, 勝見 陽介, 石松 亮一, 今任 稔彦

    第72回分析化学討論会  2012.5 

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    Event date: 2012.5

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:鹿児島市   Country:Japan  

  • DNA Dendrimer Self-Assembly for Nano-Biosensing Applications Invited International conference

    Koji Nakano

    The 2011 Global COE International Symposium on Future Molecular Systems  2011.11 

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    Event date: 2011.11

    Presentation type:Oral presentation (general)  

    Venue:Fukuoka   Country:Japan  

  • 光架橋反応と組み合わせた自己組織化DNAデンドリマー形成と化学増幅型DNAマスセンシングへの応用

    澤田貴文, 森 美詞, 中野幸二, 石松亮一, 今任稔彦

    日本分析化学会第60年会  2011.9 

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    Event date: 2011.9

    Presentation type:Oral presentation (general)  

    Venue:名古屋市   Country:Japan  

  • ホスホロチオエステル化オリゴヌクレオチドからのピボット型自己組織化膜形成とDNA修飾電極への応用

    吉永尚生, 木村壮志, 中野幸二, 石松亮一, 今任稔彦

    日本分析化学会第60年会  2011.9 

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    Event date: 2011.9

    Presentation type:Oral presentation (general)  

    Venue:名古屋市   Country:Japan  

  • 酸化還元ラベル化DNA対を用いる電位測定型遺伝子センシング

    中野幸二, 木村壮志, 石松亮一, 今任稔彦

    日本分析化学会第60年会  2011.9 

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    Event date: 2011.9

    Presentation type:Oral presentation (general)  

    Venue:名古屋市   Country:Japan  

  • Self-Assembly of Dendrimer-Like DNA and Electrical Conductivity Measurements Using Interdigitated Microelectrodes International conference

    Koji Nakano, Yoshifumi Mori, Nobuaki Soh, Toshihiko Imato

    International colloquium on "Recent progress in nanofabrications of MEMS and NEMS: Science and innovation technologies  2011.3 

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    Event date: 2011.3

    Presentation type:Oral presentation (general)  

    Venue:Fukuoka   Country:Japan  

  • Positive-Feedback-Mode Scanning Electrochemical Microscopy Imaging of DNA-Grafted Benzoquinone Polymer Cast Film for Micrometer-Sized Hybridization Biosensor Applications International conference

    Koji Nakano, Kaori Nakamura, Takayuki Kimura, Hisao Yoshinaga, Nobuaki Soh, Toshihiko Imato

    2010 International Chemical Congress of Pacific Basin Societies  2010.12 

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    Event date: 2010.12

    Presentation type:Oral presentation (general)  

    Venue:Hawaii   Country:United States  

  • Phosphorothioate DNA Self-Assembly Developed on Gold Substrates as Characterized by IR, Ellipsometry, Microgravimetric Analysis, and AFM for Application to SNPs Bioassay Taking Advantage of Supramolecular Conformation Transitions International conference

    Hisao Yoshinaga, Koji Nakano, Takayuki Kimura, Nobuaki Soh, Toshihiko Imato

    2010 International Chemical Congress of Pacific Basin Societies  2010.12 

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    Event date: 2010.12

    Presentation type:Oral presentation (general)  

    Venue:Hawaii   Country:United States  

  • AFM Visualization Read-Out od DNA Molecular Recognition for Green Bioanalysis Platform with Non-Label, Single-Molecule Detection Capability Invited International conference

    Koji Nakano

    Kumoh National Institute of Technology-Kyushu University Joint Workshop on Green Energy  2010.9 

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    Event date: 2010.9

    Presentation type:Oral presentation (general)  

    Venue:Kumoh   Country:Korea, Republic of  

  • Potentiometric Gene Sensor Based on EMF Generation from Redox-Labelled Oligonucleotide Probe Pair International conference

    Koji Nakano, Takayuki Kimura, Hisao Yoshinaga, Nobuaki Soh, Toshihiko Imato

    Faraday Discussion 149: Analysis for Healthcare Diagnostics and Theranostics  2010.9 

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    Event date: 2010.9

    Presentation type:Oral presentation (general)  

    Venue:Edinburgh   Country:United Kingdom  

  • DNA and DNA Conjugate Self-Assembly Developed on Au(111) Substrate as Characterized by Conductive Atomic Force Microscopy International conference

    Koji Nakano

    International colloquium on "Recent progress i nanotechnologies: From nanoindentation on semiconductor materials to DNA and molecular devices"  2010.3 

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    Event date: 2010.3

    Presentation type:Oral presentation (general)  

    Venue:Fukuoka   Country:Japan  

  • 光架橋性オリゴヌクレオチドを用いるデンドロンモチーフとDNAナノ分子会合体生成を利用するDNAの化学計測法

    森 美詞, 中野幸二, 宗 伸明, 今任稔彦

    日本分析化学会第58年会  2009.9 

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    Event date: 2009.9

    Presentation type:Oral presentation (general)  

    Venue:札幌市   Country:Japan  

  • Atomic Force Microscopy Imaging of Stem-Loop to Double-Helical Superstructure Transition of Probe DNA Sequence and Application to Single-Nucleotide Polymorphism Assay

    Hisao YOSHINAGA, Nobuaki SOH, Koji NAKANO, Toshihiko IMATO

    日本分析化学会第58年会  2009.9 

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    Event date: 2009.9

    Presentation type:Oral presentation (general)  

    Venue:札幌市   Country:Japan  

  • Studies of SNP assay based on single molecular imaging by using AFM International conference

    Hisao YOSHINAGA, Nobuaki SOH, Koji NAKANO, Toshihiko IMATO

    The 2nd Yonsei University BK21/Kyushu University Global-COE Joint Symposium on ‘Frontier Molecular Systems’  2009.8 

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    Event date: 2009.8

    Presentation type:Oral presentation (general)  

    Venue:Yonsei   Country:Korea, Republic of  

  • Conductive AFM Measurements on DNA and DNA Conjugate Self-Assembly Developed on Au(111) Substrate International conference

    Koji Nakano

    G-COE-Kyushu Univ. – Pusan National Univ. Joint Symposium  2009.1 

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    Event date: 2009.1

    Presentation type:Oral presentation (general)  

    Country:Japan  

  • Conductive AFM Measurement on DNA-Ferrocene Conjugate Self-Assembly Developed on Au(111) International conference

    Koji Nakano, Tomiyuki Tsuruta, Shingo Matsuo, Makoto Furue, Toshihiko Imato

    International Symposium on Surface Science and Nanotechnology  2008.11 

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    Event date: 2008.11

    Presentation type:Oral presentation (general)  

    Venue:Tokyo   Country:Japan  

  • DNAの主鎖修飾法としてのホスホロチオエステル化を利用した金基板への化学吸着固定化の研究

    木村壮志・中野幸二・宗伸明・中嶋秀・今任稔彦

    日本分析化学会第57年会  2008.9 

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    Event date: 2008.9

    Presentation type:Oral presentation (general)  

    Venue:福岡市   Country:Japan  

  • レドックス性レポーター機能を持つDNAコンジュゲートの合成とバイオセンサー応用

    吉本篤司・中野幸二・宗伸明・中嶋秀・今任稔彦

    日本分析化学会第57年会  2008.9 

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    Event date: 2008.9

    Presentation type:Oral presentation (general)  

    Venue:福岡市   Country:Japan  

  • オルト-,パラベンゾキノン置換アルカンチオール単分子膜電極における触媒的NADH酸化反応と表面依存反応活性

    中野幸二・大久保公彦・平浩昭・今任稔彦

    第69回分析化学討論会  2008.5 

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    Event date: 2008.5

    Presentation type:Oral presentation (general)  

    Venue:名古屋市   Country:Japan  

  • Cytochrome c-Attached Alkanethiol Monolayer Electrode as Characterization by AFM, IR, QCM, EC and Biosensing Transducer Applications International conference

    Koji Nakano, Tadateru Yoshitake, Yasunori Yamashita, and Toshihiko Imato

    The 9th Asian Conference on Analytical Sciences  2007.11 

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    Presentation type:Oral presentation (general)  

    Venue:Jeju Island, Jeju   Country:Korea, Republic of  

  • 糖鎖の活性化を利用したアミノアルカンチオール修飾電極へのGOD固定化と酵素単分子膜のキャラクタリゼーション

    中野幸二

    電気化学会第70回大会  2003.4 

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    Venue:東京   Country:Japan  

    Characterization for Self-Assembly of Glucose Oxidase Molecules upon Covalent Immobilization on Alkanethiol Monolayer Electrode usign Partial Oxidation of Suger Residue and Shiff-Base Formation.

  • 単分子膜修飾電極表面に固定化したタンパク質・酵素の自己組織化とバイオセンサ応用

    中野幸二

    日本分析化学会第52年会  2003.9 

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    Presentation type:Oral presentation (general)  

    Venue:仙台   Country:Japan  

    Single-Molecular Imaging for Proteins and Enzyme Molecules that Covalently Immobilized on Alkanthiols Monolayer Electrodes and Application to Biosensors

  • アルカンチオール単分子膜修飾電極に固定化したタンパク質・酵素の単一分子イメージングとバイオセンサ応用

    中野幸二

    第1回生体機能関連化学・バイオテクノロジー部会合同シンポジウム  2003.10 

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    Presentation type:Oral presentation (general)  

    Venue:熊本   Country:Japan  

    Single-Molecular Imaging for Proteins and Enzyme Molecules that Covalently Immobilized on Alkanthiols Monolayer Electrodes

  • Self-Assembling Monolayer Formation of Glucose Oxidase and Cytochrome c Covalently Attached on Alkanethiol Monolayers on Gold International conference

    K. Nakano

    International Symposium on Biomolecular Chemistry  2003.12 

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    Presentation type:Oral presentation (general)  

    Venue:兵庫県津名郡東浦町   Country:Japan  

  • DNA-Ferrocene Conjugate for Nanoelectronic Material Applications. Atomic Force Microscope-Based, Direct Measurement of Electrical Transport through DNA Molecules Invited International conference

    Koj Nakano

    The Second 21st Century COE “Towards Creating New Industries Based on Inter-Nanoscience” and 7th SANKEN International Symposium on “Hybridization of Chemistry, Biology, and Material Science”  2004.1 

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    Presentation type:Oral presentation (invited, special)  

    Venue:Osaka   Country:Japan  

  • 自己組織化単分子膜修飾電極に固定化したチトクロームc の単一分子イメージングと直接電子移動反応

    中野幸二,吉武忠輝,山下泰徳

    日本化学会第84春季年会  2004.3 

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    Presentation type:Oral presentation (general)  

    Venue:神戸   Country:Japan  

    Single-Molecular Imaging and Direct Electrochemistry of Cytochrome c Immobilized on Self-Assembled Monolayer Electrode

  • A psoralen Derivative Containing Ferrocene for Redox-labeling of DNA and Electrochemical Gene Sensor Applications International conference

    K. Nakano, S. Shirakawa, S. Taguchi

    The 10th International Meeting on Chemical Sensors  2004.7 

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    Presentation type:Oral presentation (general)  

    Venue:Tsukuba   Country:Japan  

  • Monolayer Formation of Glucose Oxidase Covalently Attached on omega-Aminoalkanethiol/Au via Activation of Carbohydrate Residue of Gox as Characterized by AFM, IR, QCM, and Biosensor Applications International conference

    Koji Nakano, Kenji Doi, Kousuke Tamura, and Yosuke Katsumi

    ASIANALYSIS VII  2004.7 

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    Presentation type:Oral presentation (general)  

    Venue:Hong Kong, SAR   Country:China  

  • 単一分子イメージングを基礎としたDNAコンジュゲートのナノ・バイオサイエンス応用 Invited

    中野幸二

    福岡市BT、NT、IT関連産業振興研究会  2004.9 

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    Presentation type:Oral presentation (general)  

    Venue:福岡   Country:Japan  

    Single-Molecular Imaging of DNA Conjugate for Nano-, Bio-Science Applications

  • Synthesis of New Psoralen Derivatives for Chemical Modification and Functionalization of DNA Duplexes as Characterized by Gel Electrophoresis, IR, QCM, Electrochemistry, and AFM International conference

    Tomiyuki Tsuruta, Yosuke Katsumi, Satoshi Shirakawa, Shinya Taguchi, and Koji Nakano

    International Symposium on Nano-organization and Function  2004.11 

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    Presentation type:Oral presentation (general)  

    Venue:Tokyo   Country:Japan  

  • Covalent Attachment of Double-Stranded DNA using Self-Assembled Monolayers Containing Psoralen as Characterized by XPS, IR, QCM, Electrochemistry, and AFM Invited International conference

    Hideshi Matsungae, Keisuke Sai, and Koji Nakano

    International Symposium on Nano-organization and Function  2004.11 

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    Presentation type:Oral presentation (general)  

    Venue:Tokyo   Country:Japan  

  • Electrical Transport Through DNA-ferrocene Conjugate Self-Assembly on Au(111) as Characterized by Conductive AFM Measurement Invited International conference

    Koji Nakano

    "The Nanoelectronics and Dynamics of DNA"  2005.8 

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    Presentation type:Oral presentation (general)  

    Venue:Honolulu, Hawaii   Country:United States  

  • DNA Conjugate Electrode Having DNA/Oligo(1,4-hydroquinone)/PG Interfacial Structure for Biosensor Applications: Characterization by Electrochemical Measurements, QCM Analysis, and AFM Imaging International conference

    K. NAKANO, G. Hirayama, M. Toguchi, K. Nakamura, K. Iwamoto, N. Soh, T. Imato

    The 8th Asian Conference on Analytical Sciences  2005.10 

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    Presentation type:Oral presentation (general)  

    Venue:Taipei   Country:Taiwan, Province of China  

  • Preparation, characterization, and atomic force microscopic imaging of new DNA ligand for single molecular hybridization assay International conference

    Hideshi MATSUNAGA, Koji NAKANO, Nobuaki SOH, Toshihiko IMATO

    PACHFICHEM2005  2005.12 

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    Presentation type:Oral presentation (general)  

    Venue:Honolulu, Hawaii   Country:United States  

  • Covalent attachment of DNA with double-strand specificity using self-assembled monolayers from psoralen International conference

    Koji NAKANO, Hideshi MATSUNAGA, Keisuke SAI

    PACHFICHEM2005  2005.12 

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    Presentation type:Oral presentation (general)  

    Venue:Honolulu, Hawaii   Country:United States  

  • 1分子検出のための遺伝子プローブ−DNAリガンド−の研究

    中野幸二

    科学技術振興機構 平成17年度プラザ福岡・FS終了報告会  2006.5 

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    Presentation type:Oral presentation (general)  

    Venue:福岡   Country:Japan  

    DNA Ligand - A New Strategy of DNA Probe for Single-Molecular Gene Assay

  • 化学気相吸着膜を利用したDNAの固定化反応:XPSによるキャラクタリゼーションとAFMアッセイへの応用

    大塚 渉,中野幸二,宗 伸明,今任稔彦

    日本分析化学会第55年会  2006.9 

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    Presentation type:Oral presentation (general)  

    Venue:大阪   Country:Japan  

    DNA Immobilization on Self-Assembling Monolayer Membrane Prepared by Chemical Vapor Adsorption Method: Characterization by XPS and Application to Hybridization Assay using AFM

  • 電気化学DNAセンサーと走査電気化学顕微鏡イメージング Invited

    中野幸二

    機器分析ワークショップ佐賀大会  2006.10 

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    Presentation type:Oral presentation (invited, special)  

    Venue:佐賀   Country:Japan  

    Electrochemical DNA Sensor and its 2D Imaging by Scanning Electrochemical Microscope

  • Electrical Transport Through DNA-Ferrocene Conjugates as Characterized by Conductive AFM Measurements Invited International conference

    Koji Nakano

    Kyushu University 21st Centrury COE "Molecular Informatics" Symposium  2006.11 

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    Presentation type:Oral presentation (general)  

    Venue:Fukuoka   Country:Japan  

  • 生体レドックス用ニトロキシルプローブの開発 Invited

    中野幸二

    東京フォーラム「先端融合医療レドックスナビ研究連合拠点」  2006.12 

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    Presentation type:Oral presentation (general)  

    Venue:東京都(経団連ホール)   Country:Japan  

    Development of Molecular Probe for NO Radical Monitoring in Biosystem

  • DNAのAFMイメージングと1分子レベルバイオアッセイ Invited

    中野幸二

    第68回分析化学討論会  2007.5 

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    Presentation type:Oral presentation (invited, special)  

    Venue:宇都宮   Country:Japan  

    AFM Imaging of DNA for Application to Single-Molecular Biosaasy

  • Molecular Beacon DNA シーケンスのAFMイメージングとコンホメーション変化に着目したハイブリダイゼーション可視化検出

    山之内洋一・中野幸二・松永英士・宗 伸明・中嶋 秀・今任稔彦

    日本分析化学会第56回年会  2007.9 

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    Presentation type:Oral presentation (general)  

    Venue:徳島   Country:Japan  

    AFM Imaging of Molecular Beacon DNA Sequence and Single-Molecular Detection of DNA Hybridization

  • DNA Stem-Loopシーケンス自己組織化膜のAFMイメージング

    中野幸二・山之内洋一・松永英士・宗 伸明・中嶋 秀・今任稔彦

    第22回生体機能関連シンポジウム  2007.9 

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    Presentation type:Oral presentation (general)  

    Venue:仙台   Country:Japan  

    AFM Imaging of Molecular Beacon DNA Sequence and Single-Molecular Detection of DNA Hybridization

  • ポリドーパミン生成に基づくELISA法の開発

    @石松亮一,@Yadoung Sumed,中野幸二

    第83回分析化学討論会  2023.5 

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    Event date: 2023.5

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:富山市   Country:Japan  

  • 電解生成したラジカルアニオンによる PTFE の表面処理法の検討

    石松 亮一, 中野 幸二

    日本分析化学会第71回年会  2022.9 

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    Event date: 2022.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:岡山   Country:Japan  

  • いくつかの芳香族炭化水素化合物のラジカルアニオンに対するプロトン付加反応速度:分光電気化学的測定

    石松 亮一, 中野 幸二

    第82回分析化学討論会  2022.5 

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    Event date: 2022.5

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:水戸市   Country:Japan  

  • 分光電気化学測定法によるピレンのラジカルアニオンダイマーの検出

    石松 亮一, 中野 幸二

    日本分析化学会第70回年会  2021.9 

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    Event date: 2021.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:神戸(オンライン)   Country:Japan  

  • ピレスロイド系殺虫剤の体内代謝物である3-phenoxybenzoic acid検出に向けたフローインジェクションELISA法の開発

    石松 亮一, #清水 慎一, 中野 幸二

    日本分析化学会第69年会  2020.9 

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    Event date: 2020.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:名古屋(オンライン)   Country:Japan  

  • 有機ラジカルイオンの吸収スペクトル測定に基づく高速検出法を用いた均一電子移動反応速度の解析

    #山口 雄輝, 石松 亮一, 中野 幸二

    日本分析化学会第68年会  2019.9 

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    Event date: 2019.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:千葉市   Country:Japan  

  • ポリアニリン生成に基づくフロー吸光 ELISA 法の開発

    石松 亮一, #清水 慎一, @森田金市, 中野幸二

    日本分析化学会第68年会  2019.9 

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    Event date: 2019.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:千葉市   Country:Japan  

  • 電極反応で生成する有機ラジカルイオンのミリ秒時間分解スペクトルの取得による均一電子移動速度反応の評価

    石松 亮一, #山口 雄輝, 中野 幸二

    第79回分析化学討論会  2019.5 

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    Event date: 2019.5

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:北九州市   Country:Japan  

  • ジフェニルエーテル系除草剤の代謝物の定量分析を目的としたポリアニリン生成に基づくフロー吸光ELISA法の開発

    #清水 慎一, 石松 亮一, @森田 金一, 中野 幸二

    日本分析化学会第67年会  2018.9 

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    Event date: 2018.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:仙台市   Country:Japan  

  • フロー電気化学発光デバイスの開発に向けたアルキルピレンの電気化学発光特性

    #岡田 拓也, 石松 亮一, 中野 幸二

    日本分析化学会第67年会  2018.9 

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    Event date: 2018.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:仙台市   Country:Japan  

  • 遺伝子のアップコンバージョン蛍光分析のためのドナー/アクセプター修飾ピロール-イミダゾールポリアミドの研究

    花山 佳祐, 中野 幸二, 石松 亮一

    日本分析化学会講演要旨集  2022.9  (公社)日本分析化学会

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    Language:Japanese  

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MISC

  • 原子間力顕微鏡(AFM)で生体分子を見る

    中野幸二

    化学と工業,第64巻第6号,460-461頁   2011.6

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    Language:Japanese   Publishing type:Article, review, commentary, editorial, etc. (scientific journal)  

  • Commentary in the general discussion in Faraday Discussion Vol. 149 Analysis for Healthcare Diagnostics and Theranostics

    Koji Nakano

    2011.1

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    Language:English  

  • バイオセンサーと医療診断

    中野幸二

    ぶんせき   2007.10

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    Language:Japanese   Publishing type:Article, review, commentary, editorial, etc. (scientific journal)  

    Biosensors for Application to Medical Diagnostics

  • Flow Immunoassay Based on Sequential Injection Using Microbeads

    RuiQi Zhang, Koji Hirakawa, Masaaki Katayama, Hizuru Nakajima, Nobuaki Soh, Koji Nakano, Toshihiko Imato

    J. Flow Injection Analysis   2006.12

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    A flow immunoassay based on a combined technique of the sequential injection with the beads injection using magnetic microbeads immobilized with antigen or antibody is described. A methodology of the present immunoassay based on chemiluminescence and electrochemical detection and its application to the determination of vitelloginin (Vg) and anionic surfactant, linear alkylbenzene sulfonate (LAS) are described. Magnetic microbeads coated with agarose gel and polylactic acid were used for immobilization of an anti-Vg antibody and an anti-LAS antibody or Vg by a conventional amino coupling method. The introduction, trapping and flushing out of the magnetic microbeads in the immunoreaction cell were controlled by the magnet and the flow of the carrier solution. The protocol of the immunoassay in the immunoreaction cell, introduction of an analyte sample, the enzyme-labeled secondary antibody or antigen and a substrate solution for chemiluminescence or electrochemical detection were sequentially carried out by the sequential injection technique. A lower detection limit around ppb level was achieved for immunoassay for Vg and LAS. The time required for an analysis was ca 15 min/sample including incubation time for immunoreaction.

    DOI: https://doi.org/10.24688/jfia.23.2_117

    Repository Public URL: https://hdl.handle.net/2324/7325077

  • DNAの固定化と遺伝子センサーへの応用

    中野幸二

    表面科学   2004.12

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    DNA Immobilization and Its Application to Gene Sensors

    DOI: https://doi.org/10.1380/jsssj.25.744

    Repository Public URL: https://hdl.handle.net/2324/7325076

  • DNA二重らせんを電子機能・構造単位とする単一分子素子

    中野幸二

    Japan NanoNet Bulletin   2004.1

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    DNA Conjugate for Single-Molecular Electronic, Bioelectrochemical Devices

  • DNAは電子材料になるのか?

    中野幸二

    化学   2003.12

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    Language:Japanese   Publishing type:Article, review, commentary, editorial, etc. (scientific journal)  

    Can A DNA Double-Helix Be Applicable To Electroconductive Material?

  • 高校生を工学に惹きつけるための工学教育コンテンツ

    高松 洋,野口博司,安福規之,田中將己,中野幸二,柁川一弘,津守不二夫,田井 明

    工学教育,65巻, 64-67   2020.5

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  • Application of Carbon Quantum Dots in Electrogenerated Chemiluminescence Sensors

    Shuai Guo, Ryoichi Ishimatsu, Koji Nakano, Toshihiko Imato

    Journal of Flow Injection Analysis, Vol. 32, No. 2, pp. 75-80   2015.12

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    Language:English   Publishing type:Article, review, commentary, editorial, etc. (scientific journal)  

    Carbon quantum dots (CQDs) are a new class of carbon nanomaterials with a size lower than 10 nm, and gain more and more attention since the discovery in 2004 due to the specific characteristics such as high solubility, robust chemical inertness, convenient for modification and high resistance to photobleaching. Compared with the traditional semiconductor quantum dots, CQDs have superior biological advantages such as low toxicity and good biocompatibility. Furthermore, CQDs have the excellent electronic properties to work as electron donors and acceptors, making this kind of material a kind of ideal emitters in the electrogenerated chemiluminescence process. In this review, we describe the recent progress in the field of electrogenerated chemiluminescence sensors using CQDs as emitters or enhancers, and in the applications of protein detection, cancer marker measurement and cell counting.

    DOI: https://doi.org/10.24688/jfia.32.2_75

    Other Link: http://jafia.kyushu-u.ac.jp/japanese/jfia/contents/32_2/HP/JFIA32(2)(2015)PP.75.pdf

    Repository Public URL: https://hdl.handle.net/2324/7325099

  • Optical Sensing Systems Suitable for Flow Analysis on Microchips

    Rong Liu, Ryoichi Ishimatsu, Koji Nakano, Toshihiko Imato

    Journal of Flow Injection Analysis, Vol. 30, No. 1, pp. 15-20   2013.12

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    Language:English   Publishing type:Article, review, commentary, editorial, etc. (scientific journal)  

    The interest in downsized optical sensing systems suitable for microflow analysis on microchips has increased considerably due to the fact that analysis on the microchip has many advantages such as analytical time including reaction time is short, less consumption of reactants and separation time is also short. The analytical system built on the microchip is portable and suitable for on-site analysis for environmental analysis and bed-side diagnosis for clinical analysis. These systems are miniature microanalysis labs fabricated on a single substrate, and have numerous applications in chemistry and life science. This review focuses on the recent advances in the optical sensing systems used for microfluidic devices including fluorescence detection, absorbance detection and chemiluminescence (CL) detection, especially on the system based on the organic light-emitting diodes (OLED) and optical photodiodes (OPD). In this review, we demonstrate the developments and applications of the downsized optical sensing systems and point out exciting new approaches, and provide future tendency on this field.

    DOI: 10.24688/jfia.30.1_15

    Other Link: http://jafia.kyushu-u.ac.jp/english/jfia/contents/30_1/30_1.html

    Repository Public URL: https://hdl.handle.net/2324/7325098

  • Sequential Injection Immunoassay for Environmental Measurements

    Nobuaki Soh, Mayumi Tanaka, Koji Hirakawa, RuiQi Zhang, Hizuru Nakajima, Koji Nakano, Toshihiko Imato

    Analytical Sciences, Vol. 27, No, 11, pp. 1069-1076   2011.11

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    Sequential injection immunoassay systems for environmental measurements based on the selective immunoreaction between antigen and antibody were described. A sequential injection analysis (SIA) technique is suitable to be applied for the procedure of enzyme-linked immunosorbent assay (ELISA), because the washing and the addition of reagent solutions can be automated by using a computer-controlled syringe pump and switching valve. We selected vitellogenin (Vg), which is a biomarker for evaluating environmental risk caused by endocrine-disrupting chemicals in the hydrosphere, and linear alkylbenzene sulfonates (LAS) and alkylphenol polyethoxylates (APEO), which are versatile surfactants, as target analytes in the flow immunoassay systems. For Vg monitoring, SIA systems based on spectrophotometric, chemiluminescence, and electrochemical determinations were constructed. On the other hand, chemiluminescence determination was applied to the detection of LAS and APEO. For APEO, an SIA system combined with surface plasmon resonance (SPR) sensor was also developed. These new sequential injection immunoassay systems are expected to be useful systems for environmental analysis.

    DOI: https://doi.org/10.2116/analsci.27.1069

    Repository Public URL: https://hdl.handle.net/2324/7325100

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Works

  • 「ぶんせき」誌2005年度表紙写真

    中野幸二

    2005.1

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    Book cover of Bunseki for 2005 volume.

Industrial property rights

Patent   Number of applications: 2   Number of registrations: 1
Utility model   Number of applications: 0   Number of registrations: 0
Design   Number of applications: 0   Number of registrations: 0
Trademark   Number of applications: 0   Number of registrations: 0

Professional Memberships

  • 錯塩化学研究懇談会

  • 日本分析化学会 化学センサー研究懇談会

  • 日本化学会 生体機能関連部会

  • Americal Chemica Society

  • 九州工学教育協会

  • The Society of Polymer Science, Japan

  • The Electrochemical Society of Japan

  • The Japan Society for Analytical Chemistry

  • The Chemical Society of Japan

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Committee Memberships

  • 日本分析化学会   代議員   Domestic

    2016.4 - 2018.3   

  • 日本分析化学会   オンライン登録委員会   Domestic

    2007.10 - 2008.9   

  • 日本分析化学会   九州支部副支部長   Domestic

    2006.4 - 2007.3   

  • 電気化学会   九州支部幹事   Domestic

    2004.2 - 2007.1   

  • 日本分析化学会   広報委員会年会・討論会オンライン登録委員   Domestic

    2003.4 - 2004.3   

  • 日本分析化学会   九州支部副支部長   Domestic

    2002.4 - 2003.3   

  • 電気化学会   九州支部庶務幹事   Domestic

    1997.4 - 1999.3   

  • 日本分析化学会   九州支部幹事   Domestic

    1995.4 - Present   

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Academic Activities

  • Screening of academic papers

    Role(s): Peer review

    2023

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    Type:Peer review 

    Number of peer-reviewed articles in foreign language journals:2

    Number of peer-reviewed articles in Japanese journals:0

    Proceedings of International Conference Number of peer-reviewed papers:0

    Proceedings of domestic conference Number of peer-reviewed papers:0

  • Screening of academic papers

    Role(s): Peer review

    2022

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    Type:Peer review 

    Number of peer-reviewed articles in foreign language journals:5

    Number of peer-reviewed articles in Japanese journals:0

    Proceedings of International Conference Number of peer-reviewed papers:0

    Proceedings of domestic conference Number of peer-reviewed papers:0

  • Screening of academic papers

    Role(s): Peer review

    2021

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    Type:Peer review 

    Number of peer-reviewed articles in foreign language journals:5

    Number of peer-reviewed articles in Japanese journals:1

    Proceedings of International Conference Number of peer-reviewed papers:0

    Proceedings of domestic conference Number of peer-reviewed papers:0

  • Screening of academic papers

    Role(s): Peer review

    2020

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    Type:Peer review 

    Number of peer-reviewed articles in foreign language journals:5

    Number of peer-reviewed articles in Japanese journals:0

    Proceedings of International Conference Number of peer-reviewed papers:0

    Proceedings of domestic conference Number of peer-reviewed papers:0

  • 日本学術振興会「卓越研究員候補者選考委員会書面審査」委員

    Role(s): Review, evaluation

    日本学術振興会  2019.10 - 2020.3

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    Type:Scientific advice/Review 

  • 座長(Chairmanship)

    第79回分析化学討論会  ( Japan ) 2019.5

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    Type:Competition, symposium, etc. 

    Number of participants:500

  • Screening of academic papers

    Role(s): Peer review

    2019

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    Type:Peer review 

    Number of peer-reviewed articles in foreign language journals:2

    Number of peer-reviewed articles in Japanese journals:0

    Proceedings of International Conference Number of peer-reviewed papers:0

    Proceedings of domestic conference Number of peer-reviewed papers:0

  • 座長(Chairmanship)

    日本分析化学会第67年会  ( Japan ) 2018.9

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    Type:Competition, symposium, etc. 

    Number of participants:900

  • Screening of academic papers

    Role(s): Peer review

    2018

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    Type:Peer review 

    Number of peer-reviewed articles in foreign language journals:10

    Number of peer-reviewed articles in Japanese journals:0

    Proceedings of International Conference Number of peer-reviewed papers:0

    Proceedings of domestic conference Number of peer-reviewed papers:0

  • 座長(Chairmanship)

    日本分析化学会第77回分析化学討論会  ( Japan ) 2017.5

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    Type:Competition, symposium, etc. 

    Number of participants:500

  • 座長(Chairmanship) International contribution

    International Congress on Analytical Sciences (ICAS2017)  ( Hainan China ) 2017.5

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    Type:Competition, symposium, etc. 

    Number of participants:400

  • Screening of academic papers

    Role(s): Peer review

    2017

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    Type:Peer review 

    Number of peer-reviewed articles in foreign language journals:10

    Number of peer-reviewed articles in Japanese journals:0

    Proceedings of International Conference Number of peer-reviewed papers:0

    Proceedings of domestic conference Number of peer-reviewed papers:0

  • 座長(Chairmanship)

    日本分析化学会第64年会  ( Japan ) 2015.9

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    Type:Competition, symposium, etc. 

  • 実行委員

    日本分析化学会第57年会  ( Japan ) 2015.9

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    Type:Competition, symposium, etc. 

    Number of participants:1,500

  • 実行委員

    平成27年度工学研究教育講演会  ( Japan ) 2015.9

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    Type:Competition, symposium, etc. 

    Number of participants:1,000

  • 文部科学省地域振興施策「地域イノベーション戦略支援プログラム」/専門評価委員

    Role(s): Review, evaluation

    文部科学省  2014.12 - 2015.11

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    Type:Scientific advice/Review 

  • Organizing Committee International contribution

    19th International Conference on Flow Injection Analysis and Related Techniques  ( Fukuoka Japan ) 2014.11 - 2014.12

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    Type:Competition, symposium, etc. 

    Number of participants:200

  • 座長(Chairmanship)

    日本分析化学会第63年会  ( Japan ) 2014.9

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    Type:Competition, symposium, etc. 

  • 座長(Chairmanship)

    日本分析化学会第62年会  ( Japan ) 2013.9 - 2012.9

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    Type:Competition, symposium, etc. 

  • 文部科学省地域振興施策「地域イノベーション戦略支援プログラム」/専門評価委員

    Role(s): Review, evaluation

    文部科学省  2013.9 - 2014.3

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    Type:Scientific advice/Review 

  • 座長(Chairmanship) International contribution

    The Twelfth Asian Conference on Analytical Sciences (ASIANALYSIS XII)  ( Fukuoka Japan ) 2013.8

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    Type:Competition, symposium, etc. 

  • Organizing Committee International contribution

    The Twelfth Asian Conference on Analytical Sciences (ASIANALYSIS XII)  ( Fukuoka Japan ) 2013.8

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    Type:Competition, symposium, etc. 

    Number of participants:300

  • 実行委員

    第72回分析化学討論会  ( Japan ) 2012.5

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    Type:Competition, symposium, etc. 

    Number of participants:600

  • Analytical Sciences

    2012.4 - 2015.3

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    Type:Academic society, research group, etc. 

  • 座長(Chairmanship)

    日本分析化学会第60年会  ( Japan ) 2011.9

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    Type:Competition, symposium, etc. 

  • 科学研究費委員会専門委員(第1段審査担当)

    Role(s): Review, evaluation

    日本学術振興会  2010.10 - 2012.3

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    Type:Scientific advice/Review 

  • 文部科学省地域振興施策「地域イノベーションクラスタープログラム(グローバル型)」/専門評価委員

    Role(s): Review, evaluation

    文部科学省  2010.9 - 2011.3

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    Type:Scientific advice/Review 

  • 文部科学省地域振興施策「知的クラスター創成事業」/専門評価委員

    Role(s): Review, evaluation

    文部科学省  2009.9 - 2009.12

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    Type:Scientific advice/Review 

  • 科学研究費委員会/専門委員

    Role(s): Review, evaluation

    日本学術振興会  2008.12 - 2009.11

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    Type:Scientific advice/Review 

  • 文部科学省地域振興施策「知的クラスター創成事業」/専門評価委員

    Role(s): Review, evaluation

    三菱総合研究所  2008.10 - 2009.3

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    Type:Scientific advice/Review 

  • 座長(Chairmanship)

    日本分析化学会第57年会  ( Japan ) 2008.9

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    Type:Competition, symposium, etc. 

  • 座長(Chairmanship)

    第69回分析化学討論会  ( Japan ) 2008.5

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    Type:Competition, symposium, etc. 

  • 科学研究費委員会/専門委員

    Role(s): Review, evaluation

    日本学術振興会  2008.1 - 2008.12

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    Type:Scientific advice/Review 

  • 実行委員

    日本分析化学会第57年会  ( Japan ) 2007.10 - Present

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    Type:Competition, symposium, etc. 

    Number of participants:800

  • 科学研究費委員会専門委員(第1段審査担当)

    Role(s): Review, evaluation

    日本学術振興会  2007.10 - 2009.3

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    Type:Scientific advice/Review 

  • 文部科学省地域振興施策「知的クラスター創成事業」/専門評価委員

    Role(s): Review, evaluation

    文部科学省  2007.10 - 2008.3

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    Type:Scientific advice/Review 

  • 座長(Chairmanship)

    日本分析化学会第56年会  ( Japan ) 2007.9 - Present

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    Type:Competition, symposium, etc. 

  • 座長(Chairmanship)

    第68回分析化学討論会  ( Japan ) 2007.5 - Present

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    Type:Competition, symposium, etc. 

  • 座長(Chairmanship)

    日本化学会第87春季年会  ( Japan ) 2007.3 - Present

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    Type:Competition, symposium, etc. 

  • 座長(Chairmanship)

    日本分析化学会第55年会  ( Japan ) 2006.9 - Present

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    Type:Competition, symposium, etc. 

  • 座長(Chairmanship)

    日本分析化学会第54年会  ( Japan ) 2005.9 - Present

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    Type:Competition, symposium, etc. 

  • 座長(Chairmanship)

    第66回分析化学討論会  ( Japan ) 2005.5 - Present

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    Type:Competition, symposium, etc. 

  • 実行委員

    電気化学会第72回大会  ( Japan ) 2005.4 - Present

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    Type:Competition, symposium, etc. 

    Number of participants:600

  • 座長(Chairmanship) International contribution

    International Symposium on Nano-organization and Function  ( Tokyo Japan ) 2004.11 - Present

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    Type:Competition, symposium, etc. 

  • 実行委員 International contribution

    International Symposium on Nano-organization and Function  ( Tokyo Japan ) 2004.11 - Present

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    Type:Competition, symposium, etc. 

    Number of participants:200

  • 座長(Chairmanship)

    第65回分析化学討論会  ( Japan ) 2004.5 - Present

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    Type:Competition, symposium, etc. 

  • 座長(Chairmanship)

    日本化学会第84春季年会  ( Japan ) 2004.3 - Present

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    Type:Competition, symposium, etc. 

  • 座長(Chairmanship)

    第1回生体機能関連化学・バイオテクノロジー部会合同シンポジウム  ( Japan ) 2003.10 - Present

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    Type:Competition, symposium, etc. 

  • 座長(Chairmanship)

    日本分析化学会第52年会  ( Japan ) 2003.9 - Present

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    Type:Competition, symposium, etc. 

  • 実行委員

    第65回分析化学討論会  ( Japan ) 2003.5 - Present

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    Type:Competition, symposium, etc. 

  • 座長(Chairmanship)

    日本化学会第81春季年会  2002.3 - Present

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    Type:Competition, symposium, etc. 

  • 実行委員

    日本分析化学会第50年会  ( Japan ) 2001.11 - Present

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    Type:Competition, symposium, etc. 

    Number of participants:1,000

  • 庶務幹事

    工業物理化学講習会  ( Japan ) 1998.10 - Present

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    Type:Competition, symposium, etc. 

    Number of participants:100

  • Organizing Committe International contribution

    The 49th Annual Meeting of The International Society of Electrochemistry  ( Kitakyushu-City Japan ) 1998.7 - Present

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    Type:Competition, symposium, etc. 

    Number of participants:500

  • 庶務幹事

    工業物理化学講習会  ( Japan ) 1997.10 - Present

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    Type:Competition, symposium, etc. 

    Number of participants:100

  • 実行委員

    第58回分析化学討論会  ( Japan ) 1997.5 - Present

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    Type:Competition, symposium, etc. 

    Number of participants:800

  • 庶務幹事

    分析化学講習会  ( Japan ) 1995.7 - Present

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    Type:Competition, symposium, etc. 

    Number of participants:100

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Research Projects

  • Human tracing based on spatio-temporal fluctuation of odor

    Grant number:22H04952  2022.4 - 2027.3

    Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (S)

    林 健司, 中野 幸二, 冨浦 洋一, 興 雄司, 竪 直也, 佐々 文洋, 石田 寛

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    Grant type:Scientific research funding

    匂いなどの揮発性化学物質情報を光情報に変換する光化学ナノプローブを2次元展開し、匂いの流れや痕跡などの空間分布を見ることができるデバイスを開発し、化学物質空間を高速・高感度、かつ高い空間分解能で可視化する。この化学イメージングデバイスは化学物質情報をハイパースペクトル情報に変換し、多種多様な匂い物質情報を網羅的に取得できる。さらに、開発したセンサデバイスの機能実証研究として、匂い情報キュレーションロボットを構築し、ガス源探索(重要例:危険物漏洩、災害現場での人探索)を実現する。

    CiNii Research

  • 匂いの時空間揺らぎ情報に基づく 人探索

    2022 - 2026

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (S)

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    Authorship:Coinvestigator(s)  Grant type:Scientific research funding

  • 世界初のDX型研究方に基づく新しい産学連携・教育システム・オープンサイエンスプラットホーム

    2020.10 - 2029.9

    日本 

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    Authorship:Coinvestigator(s) 

  • Photon Upconversion Fluorescent Probe for Sequence Selective Detection/Imagine of Double-Stranded DNA

    Grant number:20H02768  2020 - 2022

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (B)

    Nakano Koji

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    Authorship:Principal investigator  Grant type:Scientific research funding

    We report a new fluorescent probe that utilizes triplet-triplet annihilation-based photon upconversion. In particular, for analyses of the androgen-responsive sequence (ARE) in genomic DNA, we designed a type of ARE-binding pyrrole-imidazole polyamides incorporating Pt-coproporphyrin (PtCP) and anthracene (ANTH). We successfully developed a practical synthetic route in the first half of the research period. Subsequently, we studied the binding behavior to the ARE fragment and measured the fluorescence spectra for the ARE-DNA probe using a laser excitation fluorescence spectrophotometer. At last, we found that when the PtCP in the molecule was excited with excitation light at 533 nm, the molecule gave the phosphorescence of PtCP (650 nm) and the fluorescence from ANTH (480 nm). Therefore we concluded that, for the first time, we successfully developed a photon upconversion probe for DNA analyses.

    CiNii Research

  • 匂いイメージセンサによる匂い痕跡画像の要素臭プロファイル分解

    Grant number:18H03782  2018 - 2020

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (A)

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    Authorship:Coinvestigator(s)  Grant type:Scientific research funding

  • 匂いの質と空間の可視化センシング

    Grant number:15H01713  2015 - 2017

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (A)

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    Authorship:Coinvestigator(s)  Grant type:Scientific research funding

  • 九州大学「先端分子技術を核とする九州先端ものづくりセンターの構築(クリーン実験ステーション)」

    2014.4 - 2017.3

    日本 

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    Authorship:Coinvestigator(s) 

    文部科学省「先端研究施設共用促進事業」に採択された「九州大学 先端分子技術を核とする九州先端ものづくりセンターの構築(クリーン実験ステーション)」の運営委員として活動した。

  • 痛み情報の化学計測とin vivo蛍光イメージング

    Grant number:25620115  2013 - 2014

    Grants-in-Aid for Scientific Research  Grant-in-Aid for challenging Exploratory Research

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    Authorship:Principal investigator  Grant type:Scientific research funding

  • 九州大学大学院博士課程教育リーディングプログラム「分子システムデバイスコース」

    2012.10 - 2018.3

    日本 

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    Authorship:Coinvestigator(s) 

  • DNA修復の電気化学検出を利用する遺伝子の放射線変異センシング

    2012 - 2013

    科学技術振興機構 復興促進プログラム(A-STEP)探索タイプ

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    Authorship:Principal investigator  Grant type:Contract research

  • 平成23年度戦略的情報通信研究開発推進制度「匂いイメージセンサによる情報抽出に関する研究開発」

    2011.10 - 2013.9

    日本 

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    Authorship:Coinvestigator(s) 

    平成23年度戦略的情報通信研究開発推進制度に採択された「匂いイメージセンサによる情報抽出に関する研究開発」において、蛍光性ナノ粒子、化学受容性ペプチドを用いる匂い分子ナノレポーター粒子の研究を行。

  • 匂いイメージセンサによる情報創出に関する研究開発

    2011 - 2013

    平成23年度戦略的情報通信研究開発推進制度

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    Authorship:Coinvestigator(s)  Grant type:Contract research

  • 九州大学「先端分子技術を核とする九州先端ものづくりセンターの構築(クリーン実験ステーション)」

    2009.4 - 2013.3

    日本 

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    Authorship:Coinvestigator(s) 

    文部科学省「先端研究施設共用促進事業」に採択された「九州大学 先端分子技術を核とする九州先端ものづくりセンターの構築(クリーン実験ステーション)」の運営委員として活動した。

  • DNA自己組織化膜のin-situ電気回路化を利用するチップ型遺伝子センサー

    Grant number:21550085  2009 - 2011

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

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    Authorship:Principal investigator  Grant type:Scientific research funding

  • DNAチップエレクトロニクスの創製とナノバイオセンシング

    Grant number:21200034  2009 - 2011

    Japan Society for the Promotion of Science・Ministry of Education, Culture, Sports, Science and Technology  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research on Innovative Areas

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    Authorship:Principal investigator  Grant type:Scientific research funding

  • 自立性自家応答性遺伝子センサー

    2009

    科学技術振興機構 シーズ発掘試験

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    Authorship:Principal investigator  Grant type:Contract research

  • 光誘起電子移動による蛍光スイッチング機能を組み込んだ分子認識性量子ドットセンサーとバイオイメージング応用

    2008 - 2009

    九州大学先端融合COE・先端融合医療レドックスナビ研究拠点研究テーマ

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    Authorship:Principal investigator  Grant type:Contract research

  • 九州大学「未来分子システム科学」

    2007.10 - 2012.3

    日本 

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    Authorship:Coinvestigator(s) 

  • 九州大学「先端融合医療レドックスナビ研究連合拠点」

    2007.5

    日本 

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    Authorship:Coinvestigator(s) 

  • 九州大学「先端融合医療レドックスナビ研究拠点」(FS)

    2006.4 - 2007.3

    文部科学省科学技術振興調整費「先端融合領域イノベーション創出拠点の形成」 

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    Authorship:Coinvestigator(s) 

    我々生命体はその活動を様々なレドックス反応を基本として維持しているが、近年、種々の環境要因の変動により、あるいは非健康的な生活習慣によりこのレドックス代謝に異常を起こし、がん、生活習慣病、脳神経変性疾患等を引き起こすことが明らかになってきた。本プロジェクト研究では、生体レドックスを詳細に評価し、自在に操ることのできる統合技術、具体的には早期診断・治療法、治療薬の開発をめざす。

  • 1分子検出のための遺伝子プローブ−DNAリガンド−の研究

    2005.10 - 2006.3

    科学技術振興機構(日本) 

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    Authorship:Principal investigator 

    DNA Ligand-A new DNA Probe for Single Copy Dection of Specific Genes

  • 1分子検出のための遺伝子プローブ−DNAリガンド−の研究

    2005

    4) 科学技術振興機構 研究成果活用プラザ福岡 「実用化のための可能性試験

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    Authorship:Principal investigator  Grant type:Contract research

  • DNA固定化手法の開発

    2004.12 - 2005.3

    Joint research

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    Authorship:Principal investigator  Grant type:Other funds from industry-academia collaboration

  • DNAコンジュゲートを感応素子に用いるバイオエレクトロケミカルアレイ型センサ

    Grant number:15550073  2003 - 2004

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

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    Authorship:Principal investigator  Grant type:Scientific research funding

  • DNA固定化手法の開発

    2003

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    Grant type:Donation

  • 21世紀COEプログラム九州大学「分子情報科学の機能イノベーション」

    2002.10 - 2007.3

    文部科学省(日本) 

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    Authorship:Coinvestigator(s) 

    The 21st Centry COE, "Functional Innovations of Molecular Informatics"

  • DNA二重らせんを電子機能・構造単位とする単一分子素子

    2000.10 - 2003.9

    科学技術振興事業団「さきがけ」(日本) 

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    Authorship:Principal investigator 

    Investigation of DNA-Based Single Molecular Electrical Devices

  • DNA二重らせんを電子機能・構造単位とする単一分子素子

    2000 - 2003

    科学技術振興機構戦略的創造研究推進事業「さきがけ21」

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    Authorship:Principal investigator  Grant type:Contract research

  • 遺伝子ターゲッティングツールとしてのプラスミドDNA-フェロセン複合体の研究

    2000 - 2001

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

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    Authorship:Principal investigator  Grant type:Scientific research funding

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Educational Activities

  • Educational activities in the Applied Chemistry Course, Graduate School of Engineering, Kyushu University
    Electroanalytical Chemistry

    Educational activities in the Functional Materials Chemistry Course, School of Engineering, Kyushu University
    Physical Chemistry III
    Exercises in Quantum Chemistry
    Chemical English
    Security/Safety in Chemical Experiments
    Green Chemistry
    Laboratory Experiments I &II

    Educational activities in the KIKAN Education, Kyushu University
    Basic Chemical Thermodynamics
    Inorganic Materials Chemistry
    Kadai-Kyogaku


    Others
    Japanese translation publication of English textbooks
    Analytical Chemistry 7th Edition. Christian, G. D.; Dasgupta, P. K.; Schug, K. A. Wile, 2014.

Class subject

  • 物質科学工学実験第二

    2024.4 - 2024.9   First semester

  • 基礎化学熱力学Ⅰ

    2024.4 - 2024.6   Spring quarter

  • 量子化学演習

    2023.12 - 2024.2   Winter quarter

  • 物理化学演習

    2023.12 - 2024.2   Winter quarter

  • 専門英語

    2023.10 - 2024.3   Second semester

  • Experiment in Applied Chemistry IIA 応用化学実験第IIA

    2023.10 - 2023.12   Fall quarter

  • 物理化学第三

    2023.6 - 2023.8   Summer quarter

  • 物質科学工学実験第二

    2023.4 - 2023.9   First semester

  • 基礎化学熱力学Ⅰ

    2023.4 - 2023.6   Spring quarter

  • 量子化学演習

    2022.12 - 2023.2   Winter quarter

  • 物質科学工学実験第一

    2022.10 - 2023.3   Second semester

  • 物質科学工学実験第一

    2022.10 - 2023.3   Second semester

  • ナノバイオ電気分析化学

    2022.10 - 2023.3   Second semester

  • 専門英語

    2022.10 - 2023.3   Second semester

  • Experiment in Applied Chemistry IIA 応用化学実験第IIA

    2022.10 - 2022.12   Fall quarter

  • 基礎化学熱力学 II

    2022.6 - 2022.8   Summer quarter

  • 物理化学第三

    2022.6 - 2022.8   Summer quarter

  • 物質科学工学実験第二

    2022.4 - 2022.9   First semester

  • 工学概論

    2022.4 - 2022.9   First semester

  • 安全学

    2022.4 - 2022.9   First semester

  • 基礎化学熱力学 I

    2022.4 - 2022.6   Spring quarter

  • 量子化学演習

    2021.12 - 2022.2   Winter quarter

  • 専門英語

    2021.10 - 2022.3   Second semester

  • 物質科学工学実験第一

    2021.10 - 2022.3   Second semester

  • Experiment in Applied Chemistry IA 応用化学実験第IA

    2021.10 - 2021.12   Fall quarter

  • 無機物質化学Ⅱ

    2021.6 - 2021.8   Summer quarter

  • 物理化学第三

    2021.6 - 2021.8   Summer quarter

  • 物質科学工学実験第二

    2021.4 - 2021.9   First semester

  • 無機物質化学Ⅰ

    2021.4 - 2021.6   Spring quarter

  • 安全学

    2021.4 - 2021.6   Spring quarter

  • 専門英語

    2020.10 - 2021.3   Second semester

  • 物質科学工学実験第一

    2020.10 - 2021.3   Second semester

  • Electroanalytical Chemistry

    2020.10 - 2021.3   Second semester

  • 電気分析化学

    2020.10 - 2021.3   Second semester

  • 物理化学第三

    2020.6 - 2020.8   Summer quarter

  • 物質科学工学実験第二

    2020.4 - 2020.9   First semester

  • 安全学

    2020.4 - 2020.6   Spring quarter

  • 課題協学科目

    2019.10 - 2020.3   Second semester

  • 量子化学演習

    2019.10 - 2020.3   Second semester

  • 物質科学工学実験第一

    2019.10 - 2020.3   Second semester

  • グリーンケミストリー

    2019.10 - 2020.3   Second semester

  • 物理化学第三

    2019.6 - 2019.8   Summer quarter

  • 物質科学工学実験第二

    2019.4 - 2019.9   First semester

  • 安全学

    2019.4 - 2019.9   First semester

  • 量子化学演習

    2018.10 - 2019.3   Second semester

  • 電気分析化学

    2018.10 - 2019.3   Second semester

  • 物質科学工学実験第一

    2018.10 - 2019.3   Second semester

  • 物理化学第三

    2018.4 - 2018.9   First semester

  • 安全学

    2018.4 - 2018.9   First semester

  • 物質科学工学実験第二

    2018.4 - 2018.9   First semester

  • 安全学

    2017.10 - 2018.3   Second semester

  • 量子化学演習

    2017.10 - 2018.3   Second semester

  • 物質科学工学実験第一

    2017.10 - 2018.3   Second semester

  • 応用物理化学

    2017.4 - 2017.9   First semester

  • 物質科学工学実験第二

    2017.4 - 2017.9   First semester

  • 物理化学第三

    2017.4 - 2017.9   First semester

  • 無機物質化学

    2017.4 - 2017.9   First semester

  • 電気分析化学

    2016.10 - 2017.3   Second semester

  • 物質科学工学概論第一

    2016.10 - 2017.3   Second semester

  • 物質科学工学実験第一

    2016.10 - 2017.3   Second semester

  • 安全学

    2016.10 - 2017.3   Second semester

  • 化学システム工学演習第二B

    2016.4 - 2017.3   Full year

  • 物質科学工学実験第二

    2016.4 - 2016.9   First semester

  • 物理化学第三

    2016.4 - 2016.9   First semester

  • 基礎化学熱力学

    2016.4 - 2016.9   First semester

  • 安全学

    2015.10 - 2016.3   Second semester

  • 化学システム工学演習第二B

    2015.4 - 2016.3   Full year

  • 物質科学工学実験第二

    2015.4 - 2015.9   First semester

  • 物理化学演習

    2015.4 - 2015.9   First semester

  • 物質科学工学概論第一

    2015.4 - 2015.9   First semester

  • 電気分析化学

    2014.10 - 2015.3   Second semester

  • 安全学

    2014.10 - 2015.3   Second semester

  • 化学システム工学演習第二B

    2014.4 - 2015.3   Full year

  • 物質科学工学実験第二

    2014.4 - 2014.9   First semester

  • 物理化学演習

    2014.4 - 2014.9   First semester

  • 自然科学総合実験

    2014.4 - 2014.9   First semester

  • 物質科学工学概論第一

    2014.4 - 2014.9   First semester

  • 化学システム工学演習第二B

    2013.4 - 2014.3   Full year

  • 物質科学工学実験第二

    2013.4 - 2013.9   First semester

  • 物理化学演習

    2013.4 - 2013.9   First semester

  • 先端工学B

    2013.4 - 2013.9   First semester

  • 自然科学総合実験

    2013.4 - 2013.9   First semester

  • 物質科学工学概論第二

    2013.4 - 2013.9   First semester

  • 電気分析化学

    2012.10 - 2013.3   Second semester

  • 化学システム工学演習第二B

    2012.4 - 2013.3   Full year

  • 物理化学演習

    2012.4 - 2012.9   First semester

  • 安全学

    2011.10 - 2012.3   Second semester

  • 化学システム工学演習第二B

    2011.4 - 2012.3   Full year

  • 自然科学総合実験

    2011.4 - 2011.9   First semester

  • 物理化学演習

    2011.4 - 2011.9   First semester

  • 物質科学工学概論第二

    2011.4 - 2011.9   First semester

  • 電気分析化学

    2010.10 - 2011.3   Second semester

  • 化学システム工学演習第二B

    2010.4 - 2011.3   Full year

  • 物質科学工学概論第二

    2010.4 - 2010.9   First semester

  • 物理化学演習

    2010.4 - 2010.9   First semester

  • 日韓共同理工系学部留学生予備教育科目・化学

    2009.10 - 2010.3   Second semester

  • 化学システム工学演習第二B

    2009.4 - 2010.3   Full year

  • 物質科学工学概論第二

    2009.4 - 2009.9   First semester

  • 物理化学演習

    2009.4 - 2009.9   First semester

  • 安全学

    2008.10 - 2009.3   Second semester

  • 電気分析化学

    2008.10 - 2009.3   Second semester

  • 物理化学演習

    2008.4 - 2008.9   First semester

  • コアセミナー

    2008.4 - 2008.9   First semester

  • 物質科学工学概論第二

    2008.4 - 2008.9   First semester

  • 物理化学演習

    2007.4 - 2007.9   First semester

  • 物質科学工学概論第二

    2007.4 - 2007.9   First semester

  • 電気分析化学

    2006.10 - 2007.3   Second semester

  • 物理化学演習

    2006.4 - 2006.9   First semester

  • 日韓共同理工系学部留学生予備教育科目・化学

    2005.10 - 2006.3   Second semester

  • 物理化学演習

    2005.4 - 2005.9   First semester

  • 創造工学演習

    2005.4 - 2005.9   First semester

  • 電気分析化学

    2004.10 - 2005.3   Second semester

  • 物理化学演習

    2004.4 - 2004.9   First semester

  • 分析化学

    2003.10 - 2004.3   Second semester

  • 電気分析化学

    2003.4 - 2003.9   First semester

  • 基礎物理化学第二及び演習

    2003.4 - 2003.9   First semester

  • 電気分析化学

    2002.4 - 2002.9   First semester

  • 基礎化学実験

    2002.4 - 2002.9   First semester

  • 創造工学演習

    2002.4 - 2002.9   First semester

  • 基礎物理化学第二及び演習

    2002.4 - 2002.9   First semester

▼display all

FD Participation

  • 2023.7   Role:Participation   Title:【シス情FD】若手教員の研究紹介⑨

    Organizer:[Undergraduate school/graduate school/graduate faculty]

  • 2023.3   Role:Participation   Title:全学FD:メンタルヘルス講演会

    Organizer:University-wide

  • 2023.3   Role:Participation   Title:【シス情FD】独・蘭・台湾での産学連携を垣間見る-Industy 4.0・量子コンピューティング・先端半導体-

    Organizer:[Undergraduate school/graduate school/graduate faculty]

  • 2023.3   Role:Participation   Title:全学FD「M2Bシステムの使い方」(3/9~オンデマンド)

    Organizer:University-wide

  • 2023.3   Role:Participation   Title:TF(ティーチング・フェロー)経験を通じて大学院生の教育能力を高める

    Organizer:[Undergraduate school/graduate school/graduate faculty]

  • 2022.11   Role:Participation   Title:【工学・シス情】教職員向け知的財産セミナー(FD)

    Organizer:[Undergraduate school/graduate school/graduate faculty]

  • 2022.7   Role:Participation   Title:【シス情FD】若手教員による研究紹介⑤

    Organizer:[Undergraduate school/graduate school/graduate faculty]

  • 2022.6   Role:Participation   Title:高校訪問事業(出前講義、入試説明)に関するFD

    Organizer:[Undergraduate school/graduate school/graduate faculty]

  • 2020.4   Role:Participation   Title:Moodleを利用したe-Learning実例報告(九州大学電気情報において)

    Organizer:[Undergraduate school/graduate school/graduate faculty]

  • 2019.4   Role:Participation   Title:課題協学科目FD

    Organizer:University-wide

  • 2019.3   Role:Participation   Title:平成33年度入学者選抜改革 【一般選抜における主体性等評価について】

    Organizer:University-wide

  • 2017.2   Role:Participation   Title:QRECの教育プログラム

    Organizer:[Undergraduate school/graduate school/graduate faculty]

  • 2016.6   Role:Participation   Title:ハラスメント防止のための研修会

    Organizer:University-wide

  • 2015.8   Role:Participation   Title:第6回応用化学部門FD GPA制度、クォーター制への移行について

    Organizer:Undergraduate school department

  • 2015.7   Role:Participation   Title:第2回全学FD 教育の質向上プログラム(EEP)成果発表会

    Organizer:University-wide

  • 2015.1   Role:Participation   Title:平成27年度第2回工学部(府)FD 「4学期制」

    Organizer:[Undergraduate school/graduate school/graduate faculty]

  • 2015.1   Role:Participation   Title:平成27年度第1回工学部(府)FD 『English Learning in Teaching English (ELITE) ー平成26年度EEP研修報告ー』

    Organizer:[Undergraduate school/graduate school/graduate faculty]

  • 2014.11   Role:Participation   Title:第5回応用化学部門FD

    Organizer:Undergraduate school department

  • 2014.7   Role:Participation   Title:新GPA制度実施のためのFD

    Organizer:University-wide

  • 2014.7   Role:Participation   Title:平成26年度第1回工学部(府)FD 『English Learning in Teaching English (ELITE) ー平成25年度EEP研修報告ー』

    Organizer:[Undergraduate school/graduate school/graduate faculty]

  • 2014.3   Role:Participation   Title:平成25年度第3回工学部(府)FD「学生のメンタルヘルスーキャンパスライフ・健康支援センターから」および「工学講義賞受賞者によるご講演」

    Organizer:[Undergraduate school/graduate school/graduate faculty]

  • 2013.7   Role:Participation   Title:第五回応用化学部門教育FD

    Organizer:Undergraduate school department

  • 2013.6   Role:Participation   Title:平成25年度第1回工学部(府)FD「基幹教育と基幹教育カリキュラム」

    Organizer:[Undergraduate school/graduate school/graduate faculty]

  • 2013.5   Role:Participation   Title:第四回応用化学部門教育FD

    Organizer:Undergraduate school department

  • 2013.3   Role:Participation   Title:平成24年度第2回工学部(府)FD「工学講義賞ご受賞の先生からの授業の在り方のご紹介」

    Organizer:[Undergraduate school/graduate school/graduate faculty]

  • 2013.1   Role:Participation   Title:平成24年度工学研究院講習会「女性則の改正と今後の労働衛生、及びハラスメント防止に関する研修会」

    Organizer:[Undergraduate school/graduate school/graduate faculty]

  • 2012.3   Role:Participation   Title:平成23年度工学部(府)FD EEP活動報告

    Organizer:[Undergraduate school/graduate school/graduate faculty]

  • 2011.5   Role:Participation   Title:第三回応用化学部門教育FD

    Organizer:Undergraduate school department

  • 2011.1   Role:Participation   Title:英語論文作成・プレゼンテーションスキル涵養のための英語クラス

    Organizer:[Undergraduate school/graduate school/graduate faculty]

  • 2010.9   Role:Participation   Title:平成22年度第3回全学FD:学生の「学力」と「学ぶ力」はどのように変わったか〜今日の初年次学生の学習特性について〜

    Organizer:University-wide

  • 2010.5   Role:Participation   Title:平成22年度工学教育シンポジウム〜工学部における国際戦略〜

    Organizer:[Undergraduate school/graduate school/graduate faculty]

  • 2007.7   Role:Participation   Title:認証評価で見出された九州大学の教育課題と今後の対応

    Organizer:University-wide

  • 2007.3   Role:Participation   Title:GPA制度が目指すこと

    Organizer:University-wide

  • 2002.7   Role:Participation   Title:基礎科学教育部会(生物)

    Organizer:University-wide

▼display all

Visiting, concurrent, or part-time lecturers at other universities, institutions, etc.

  • 2006  県立広島大学  Classification:Part-time lecturer  Domestic/International Classification:Japan 

    Semester, Day Time or Duration:前期,隔週金曜日3・4限

Other educational activity and Special note

  • 2010  Class Teacher  全学

  • 2009  Class Teacher  全学

Outline of Social Contribution and International Cooperation activities

  • 2003年度〜2019年度における社会貢献、国際連携活動として、以下の学会や支部大会、国際シンポジウムにおける実行委員として参画した。
    ・令和元年度 第79回分析化学討論会
    ・平成28年度 日本分析化学会九州支部60周年記念会
    ・平成27年度 日本分析化学会第67年会
    ・平成18年度 日本分析化学会九州支部50周年記念会
    ・平成18年度 日本分析化学会九州支部機器分析ワークショップ
    ・平成17年度 電気化学会第72回大会
    ・平成16年度 International Symposium on Nano-organization and Function
    ・平成16年度 第65回分析化学討論会

Social Activities

  • 化学環境学とバイオセンシング

    一般財団法人 化学物質評価研究機構(CERI) 寄付講座・九州大学 大学院工学研究院 応用化学部門  福岡市産学連携交流センター  2023.6

     More details

    Audience:General, Scientific, Company, Civic organization, Governmental agency

    Type:Lecture

  • 化学環境学とバイオセンシング

    一般財団法人 化学物質評価研究機構(CERI) 寄付講座・九州大学 大学院工学研究院 応用化学部門  福岡市産学連携交流センター  2022.6

     More details

    Audience:General, Scientific, Company, Civic organization, Governmental agency

    Type:Lecture

  • 化学環境学とバイオセンシング

    一般財団法人 化学物質評価研究機構(CERI) 寄付講座・九州大学 大学院工学研究院 応用化学部門  福岡市産学連携交流センター  2021.6

     More details

    Audience:General, Scientific, Company, Civic organization, Governmental agency

    Type:Lecture

  • 化学環境学とバイオセンシング

    一般財団法人 化学物質評価研究機構(CERI) 寄付講座・九州大学 大学院工学研究院 応用化学部門  福岡市産学連携交流センター  2020.7

     More details

    Audience:General, Scientific, Company, Civic organization, Governmental agency

    Type:Lecture

  • 化学環境学とバイオセンシング

    一般財団法人 化学物質評価研究機構(CERI) 寄付講座・九州大学 大学院工学研究院 応用化学部門  福岡市産学連携交流センター  2019.6

     More details

    Audience:General, Scientific, Company, Civic organization, Governmental agency

    Type:Lecture

  • 化学環境学とバイオセンシング

    一般財団法人 化学物質評価研究機構(CERI) 寄付講座・九州大学 大学院工学研究院 応用化学部門  福岡市産学連携交流センター  2018.6

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    Audience:General, Scientific, Company, Civic organization, Governmental agency

    Type:Lecture

  • 環境分析と資源・エネルギー循環 ―安全・安心な生活を目指して-

    九州大学大学院工学研究院附属循環型社会システム工学研究センター  九州大学伊都キャンパス  2016.11

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    Audience:General, Scientific, Company, Civic organization, Governmental agency

    Type:Lecture

  • 研究指導/化学クラブ生徒を対象にした研修会(福岡県高等学校化学部協賛)において生徒へのアドバイザリーを行った。

    中村学園三陽高等学校、及び鞍手高校  2016.10

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    Audience:Infants, Schoolchildren, Junior students, High school students

    Type:Other

  • 研究指導/化学クラブ生徒を対象にした研修会(福岡県高等学校化学部協賛)において生徒へのアドバイザリーを行った。

    福岡工業大学附属城東高等学校、および修猷館高等学校  2015.11

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    Audience:Infants, Schoolchildren, Junior students, High school students

    Type:Other

  • 研究指導/化学クラブ生徒を対象にした研修会(福岡県高等学校化学部協賛)において生徒へのアドバイザリーを行った。

    福岡県立鞍手高等学校、および福岡県立春日高等学校  2014.11

     More details

    Audience:Infants, Schoolchildren, Junior students, High school students

    Type:Other

  • 研究指導/化学クラブ生徒を対象にした研修会(福岡県高等学校化学部協賛)において生徒へのアドバイザリーを行った。

    福岡県立修猷館高等学校、および福岡県立城南高等学校  2013.11

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    Audience:Infants, Schoolchildren, Junior students, High school students

    Type:Other

  • 研究指導/科学技術振興機構スーパーサイエンスハイスクール指定校の研究活動について研究課題の選定、研究の指針、および結果の取りまとめについて指導した。

    福岡県立城南高等学校  2013.5

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    Audience:Infants, Schoolchildren, Junior students, High school students

    Type:Other

  • 研究指導

    福岡県立城南高校化学クラブによる訪問を受け、同校高校生によるスーパーサイエンスハイスクール活動について、具体的な実験内容や課題についての説明を受け、その対応等についてアドバイスした。  2013.2

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    Audience:Infants, Schoolchildren, Junior students, High school students

    Type:Other

  • 研究指導

    福岡県立城南高校化学クラブによる訪問を受け、同校高校生によるスーパーサイエンスハイスクール活動について、具体的な実験内容や課題についての説明を受け、その対応等についてアドバイスした。  2012.10

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    Audience:Infants, Schoolchildren, Junior students, High school students

    Type:Other

  • 研究指導

    福岡県立城南高校化学クラブによる訪問を受け、同校高校生によるスーパーサイエンスハイスクール活動について、具体的な実験内容や課題についての説明を受け、その対応等についてアドバイスした。  2011.10

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    Audience:Infants, Schoolchildren, Junior students, High school students

    Type:Other

  • 研究指導

    福岡県立城南高校化学クラブによる訪問を受け、同校高校生によるスーパーサイエンスハイスクール活動について、具体的な実験内容や課題についての説明を受け、その対応等についてアドバイスした。  2011.1

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    Audience:Infants, Schoolchildren, Junior students, High school students

    Type:Other

  • 研究指導

    福岡県立城南高校化学クラブによる訪問を受け、同校高校生によるスーパーサイエンスハイスクール活動について、具体的な実験内容や課題についての説明を受け、その対応等についてアドバイスした。  2010.10

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    Audience:Infants, Schoolchildren, Junior students, High school students

    Type:Other

  • 職場見学会

    玄洋中学校からの職場見学を受け入れ、講義や見学会、体験実験を実施した。  2010.9

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    Audience:Infants, Schoolchildren, Junior students, High school students

    Type:Other

  • 見学会

    福岡舞鶴中学校・高等学校による大学見学会において応用化学部門における研究活動を紹介した。  2010.9

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    Audience:Infants, Schoolchildren, Junior students, High school students

    Type:Other

  • 福岡市内の小学生、中学生を対象にした福岡市産学連携交流センターセミナーにおいて、「遺伝子の世界」と題する講演を行い、遺伝子と私たちの生活との関わり合いについて分かりやすく解説した。

    九州大学未来化学創造センター、および福岡市  福岡市産学連携交流センター  2010.8

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    Audience:General, Scientific, Company, Civic organization, Governmental agency

    Type:Seminar, workshop

  • 研究指導

    福岡県立城南高校化学クラブによる訪問を受け、同校高校生によるスーパーサイエンスハイスクール活動についてディスカッションを行った。  2010.7

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    Audience:Infants, Schoolchildren, Junior students, High school students

    Type:Other

  • 見学会・研究指導

    福岡県立修猷館高校化学クラブによる訪問を受け、同校における課外活動の成果について説明を受けるとともに内容についてディスカッションを行った。  2009.11

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    Audience:Infants, Schoolchildren, Junior students, High school students

    Type:Other

  • 見学会

    福岡舞鶴中学校・高等学校による大学見学会において応用化学部門における研究活動を紹介した。  2009.9

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    Audience:Infants, Schoolchildren, Junior students, High school students

    Type:Other

  • 見学会

    鹿児島玉龍中学校による大学見学会において応用化学部門の説明を行った。  2009.3

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    Audience:Infants, Schoolchildren, Junior students, High school students

    Type:Other

  • 科学技術振興機構サイエンス・パートナーシップ・プロジェクト企画として、九州大学伊都キャンパスにおいて、高校科学教員を対象とした研修事業「高校科学教育のための最先端化学実習コース」を実施した

    科学技術振興機構  2008.8

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    Audience:Infants, Schoolchildren, Junior students, High school students

    Type:Seminar, workshop

  • 科学技術振興機構サイエンス・パートナーシップ・プロジェクト企画として、九州大学伊都キャンパスにおいて、高校科学教員を対象とした研修事業「高校科学教育のための最先端化学実習コース」を実施した

    科学技術振興機構  2007.8

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    Audience:Infants, Schoolchildren, Junior students, High school students

    Type:Seminar, workshop

  • 講演「光を当てると色が変わる」「やわらかくて、プルプルしているもの:ゲルの科学」「地球をきれいにする魔法の粉」「プラスチックエレクトロニクス」、および関連の実習4件からなる公開講座を実施した。

    九州大学工学部  九州大学伊都キャンパス  2006.8

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    Audience:General, Scientific, Company, Civic organization, Governmental agency

    Type:Lecture

  • 科学技術振興機構サイエンス・パートナーシップ・プロジェクト企画として、九州大学伊都キャンパスにおいて、高校科学教員を対象とした研修事業「高校科学教育のための最先端化学実習コース」を実施した

    科学技術振興機構  2006.8

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    Audience:Infants, Schoolchildren, Junior students, High school students

    Type:Seminar, workshop

  • 九州大学工学部主催による出張講義を実施した。

    福岡県立嘉穂高等学校  2005.7

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    Audience:Infants, Schoolchildren, Junior students, High school students

    Type:Seminar, workshop

  • 上記公開講座にて、「ナノテクノロジーで観るバイオ分子の世界」に関して講義するとともに、関連の技術展示を行った。

    九州大学工学部  九州大学箱崎キャンパス  2004.8

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    Audience:General, Scientific, Company, Civic organization, Governmental agency

    Type:Lecture

  • 九州大学説明会:九州大学主催のオープンキャンパス企画について、応用化学部門責任者として実施した。

    九州大学  2004.8

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    Audience:Infants, Schoolchildren, Junior students, High school students

    Type:Seminar, workshop

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Travel Abroad

  • 2017.5 - Present

    Staying countory name 1:China   Staying institution name 1:Sheraton Haikou Hotel, Haikou

  • 2016.12 - Present

    Staying countory name 1:Thailand   Staying institution name 1:The Empress Hotel, Chiang Mai

  • 2015.12 - Present

    Staying countory name 1:Korea, Republic of   Staying institution name 1:Ramada Plaza Hotel, Jeju

  • 2012.3

    Staying countory name 1:United States   Staying institution name 1:Hawaii University at Manoa

  • 2010.12 - Present

    Staying countory name 1:United States   Staying institution name 1:Hawaii Convention Center

  • 2010.9 - Present

    Staying countory name 1:Korea, Republic of   Staying institution name 1:Kumoh National Institute of Technology

  • 2010.9 - Present

    Staying countory name 1:United Kingdom   Staying institution name 1:University of Edinburgh

  • 2007.11 - Present

    Staying countory name 1:Korea, Republic of   Staying institution name 1:Ramada Plaza Jeju

  • 2005.12 - Present

    Staying countory name 1:United States   Staying institution name 1:不明

  • 2005.10 - Present

    Staying countory name 1:Taiwan, Province of China   Staying institution name 1:不明

  • 2005.8 - Present

    Staying countory name 1:United States   Staying institution name 1:不明

  • 2004.7 - Present

    Staying countory name 1:Other   Staying institution name 1:不明

  • 2000.3 - Present

    Staying countory name 1:United States   Staying institution name 1:不明

  • 1999.5 - Present

    Staying countory name 1:China   Staying institution name 1:不明

  • 1995.10 - 1996.9

    Staying countory name 1:United States   Staying institution name 1:North Carolina State University

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