2025/04/17 更新

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写真a

ミヤザキ ヨシユキ
宮﨑 義之
MIYAZAKI YOSHIYUKI
所属
農学研究院 准教授
職名
准教授
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外部リンク

学位

  • 博士(農学)

経歴

  • 平成22年1月1日〜平成23年7月31日 特定非営利活動法人NPOフコイダン研究所 主任研究員 平成23年8月1日〜平成26年9月30日 特定非営利活動法人NPOフコイダン研究所 理事長 平成26年10月1日〜現在 特定非営利活動法人NPOフコイダン研究所 理事   

    平成22年1月1日〜平成23年7月31日 特定非営利活動法人NPOフコイダン研究所 主任研究員 平成23年8月1日〜平成26年9月30日 特定非営利活動法人NPOフコイダン研究所 理事長 平成26年10月1日〜現在 特定非営利活動法人NPOフコイダン研究所 理事

  • 平成15年4月1日〜平成16年4月30日 科学技術振興事業団(PRESTO) 研究員 平成16年5月1日〜平成21年12月31日 佐賀大学 医学部 分子生命科学講座 助教   

研究テーマ・研究キーワード

  • 研究テーマ: 食品成分による免疫調節作用に関する研究 硫酸化多糖類の免疫調節機能に関する研究 多糖類の新規分析手法の開発

    研究キーワード: 多糖類 機能性食品 免疫制御 食品分析

    研究期間: 2016年4月 - 2021年3月

論文

  • Profiling sulfate content of polysaccharides in seaweed species using a ligand-assisted 1H-NMR assay 査読 国際誌

    #Juneha Bak, Yoshiyuki Miyazaki, @Hayato Nakano, Toshiro Matsui

    Food Science and Technology Research   27 ( 3 )   505 - 510   2021年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.3136/fstr.27.505

  • Ligand-aided 1H Nuclear Magnetic Resonance Spectroscopy for Non-destructive Estimation of Sulfate Content in Sulfated Saccharides 査読 国際誌

    #Juneha BAK, Yoshiyuki MIYAZAKI, @Hayato NAKANO, Toshiro MATSUI

    Analytical Sciences   36 ( 10 )   1269 - 1274   2020年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.2116/analsci.20P163

  • The cooperative induction of macrophage activation by fucoidan derived from Cladosiphon okamuranus and β-glucan derived from Saccharomyces cerevisiae 査読

    Yoshiyuki Miyazaki, Yuri Iwaihara, Juneha Bak, Hayato Nakano, Shugo Takeuchi, Hideaki Takeuchi, Toshiro Matsui, Daisuke Tachikawa

    Biochemical and Biophysical Research Communications   516 ( 1 )   245 - 250   2019年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    The present study investigated immune stimulatory effects of Cladosiphon okamuranus-derived fucoidan to activate murine macrophage-like cell line RAW264, and the functional relationship with zymosan, a Saccharomyces cerevisiae-derived β-glucan. The production of nitric oxide (NO) and tumor necrosis factor-α (TNF-α) in RAW264 cells were remarkably enhanced in the presence of 10 μg/mL fucoidan, and the stimulatory effects of fucoidan were maximally augmented in combinational treatment with 500 ng/mL zymosan, whereas any TLR ligands had no those effects. Confocal microscopic analyses suggested that fucoidan bound on plasma membrane, and it was estimated that some cell surface molecules acted as receptor for fucoidan because cytochalasin D, an inhibitor of phagocytosis, did not affect the immune enhancing activities, whereas methyl-β-cyclodextrin (MβCD), a general agent for disruption of lipid rafts, diminished that. Furthermore, it was revealed that the additive effects of zymosan on the immune activation with fucoidan was thought to be mediated by dectin-1 based on the results with dectin-1-knockdown RAW264 cells. All of results suggested that fucoidan and some kinds of β-glucan would cooperatively reinforce the activity of innate immune cells via interactive receptor crosstalk.

    DOI: 10.1016/j.bbrc.2019.06.010

  • ザクロ酢のヒスタミン放出抑制活性におけるポリフェノール化合物の関与 査読

    宮﨑 義之, 倉田 有希江, 古賀 裕章, 山口 智, 立花 宏文, 山田 耕路

    日本食品科学工学会   63 ( 2 )   63 - 69   2016年2月

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)  

    本研究では,食酢の体調調節機能の解明を目的として,各種果実酢のヒスタミン放出抑制活性について検討した.まず,山ブドウ,ハスカップ,ブルーベリー,ザクロを原料とする4種類の果実酢がラット好塩基球様白血病細胞株RBL-2H3のヒスタミン放出に及ぼす影響を検討したところ,各果実酢がヒスタミン放出抑制活性を有することが明らかとなり,特にザクロ酢で極めて強い活性が認められた.そこで,Diaion HP20を用いてザクロ酢中の生理活性成分のクロマト分離を試みた結果,50% EtOH溶出画分に強いヒスタミン放出抑制活性が認められた.さらに,本50% EtOH溶出画分を液-液抽出によって分画し,ザクロ酢には水溶性の異なる複数のヒスタミン放出抑制成分が存在することを明らかにした.また,各画分のヒスタミン放出抑制活性がPVPPで処理することによってほぼ完全に消失したことから,ポリフェノール化合物が主要な活性成分であることが示唆された.これらの結果から,ザクロ酢にはヒスタミン放出抑制に寄与する複数のポリフェノール成分が存在し,他の食酢と比較して強い抗アレルギー作用を発揮する可能性があることが示唆された.

    DOI: 10.3136/nskkk.63.63

  • ブロッコリーのビタミンC, S-メチルメチオニン, ポリフェノール含有量の部位別解析と細胞機能への影響 招待 査読

    上田 京子, 塚谷 忠之, 村山 加奈子, 倉田 有希江, 竹田 絵里, 大塚 崇文, 高井 美佳, 宮﨑 義之, 立花 宏文, 山田 耕路

    日本食品科学工学会   62 ( 5 )   242 - 249   2015年5月

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)  

    We studied the nutritional components and functional activities of 6 parts (flower bud, stem, lower main stalk, leaf stalk, leaf, root) of raw whole broccoli (Brassica oleracea var. Italica). Flower buds showed the highest levels of vitamin C (188.2mg/100g) and S-methylmethionine (16.7mg/100g). The buds contained 64.9mg/100g of polyphenols. Moreover, they exhibited the highest histamine release inhibitory activity. The vitamin C, S-methylmethionine and polyphenol contents of the leaves were 18%, 29% and 3.1 times those of flower buds, respectively. The leaves showed histamine release inhibitory activity and leukotriene release inhibitory activity. Additionally, the leaves tended to decrease IgE production. The leaves also showed anti-allergic activity as compared with flower buds. The vitamin C, S-methylmethionine and polyphenol contents of the roots were 13%, 25% and 83% of those of flower buds, respectively. Notably, aqueous extracts of roots produced the highest proliferative arrest in MCF-7 human breast cancer cells. These results suggest that useful components exist not only in the edible flower buds but in all parts of broccoli.

    DOI: 10.3136/nskkk.62.242

  • The enhancing effect of fucoidan derived from Undaria pinnatifida on immunoglobulin production by mouse spleen lymphocytes 査読 国際誌

    Mika Takai, Yoshiyuki Miyazaki, Hirofumi Tachibana, Koji Yamada

    Bioscience, Biotechnology, and Biochemistry   78 ( 10 )   1743 - 1747   2014年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    In this study, we revealed that a Mekabu (Udaria pinnantifida) extract enhanced immunoglobulin (Ig) production of mouse spleen lymphocytes. Furthermore, it was suggested that water-soluble and high molecular weight ingredients in the Mekabu extract have significant enhancing effect on Ig production. Therefore, fucoidan was estimated as the active component.

    DOI: 10.1080/09168451.2014.930323

  • An ITAM-Syk-CARD9 signalling axis triggers contact hypersensitivity by stimulating il-1 production in dendritic cells 査読

    Shinsuke Yasukawa, Yoshiyuki Miyazaki, Chika Yoshii, Mako Nakaya, Naoko Ozaki, Shuji Toda, Etsushi Kuroda, Ken Ichi Ishibashi, Tomoharu Yasuda, Yohei Natsuaki, Fumika Mi-Ichi, Eíichi Iizasa, Takeshi Nakahara, Masanori Yamazaki, Kenji Kabashima, Yoichiro Iwakura, Toshiyuki Takai, Takashi Saito, Tomohiro Kurosaki, Bernard Malissen, Naohito Ohno, Masutaka Furue, Hiroki Yoshida, Hiromitsu Hara

    Nature communications   5   2014年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    A variety of reactive organic compounds, called haptens, can cause allergic contact dermatitis. However, the innate immune mechanisms by which haptens stimulate dendritic cells (DCs) to sensitize T cells remain unclear. Here we show that the coupling of ITAM-Syk-CARD9 signalling to interleukin-1 (IL-1) secretion in DCs is crucial for allergic sensitization to haptens. Both MyD88 and Caspase recruitment domain-containing protein 9 (CARD9) signalling are required for contact hypersensitivity (CHS). Naïve T cells require signals received through IL-1R1-MyD88 for effector differentiation, whereas DCs require CARD9 and spleen tyrosine kinase (Syk) signalling for hapten-induced IL-1α /Î 2 secretion and their ability to prime T cells. DC-specific deletion of CARD9, DAP12, Syk or NLRP3, but not MyD88, is sufficient to abolish CHS. All tested haptens, but not irritants, can induce Syk activation, leading to both the CARD9/BCL10-dependent pro-IL-1 synthesis (signal1) and reactive oxygen species-mediated NLRP3 inflammasome activation (signal2), required for IL-1 secretion. These data unveil an innate immune mechanism crucial for allergic contact sensitization to chemical compounds.

    DOI: 10.1038/ncomms4755

  • Interleukin 27 inhibits atherosclerosis via immunoregulation of macrophages in mice 査読

    Tetsuaki Hirase, Hiromitsu Hara, Yoshiyuki Miyazaki, Noriko Ide, Ai Nishimoto-Hazuku, Hirokazu Fujimoto, Christiaan J M Saris, Hiroki Yoshida, Koichi Node

    American Journal of Physiology - Heart and Circulatory Physiology   305 ( 3 )   2013年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Chronic inflammation in arterial wall that is driven by immune cells and cytokines plays pivotal roles in the development of atherosclerosis. Interleukin 27 (IL-27) is a member of the IL-12 family of cytokines that consists of IL-27p28 and Epstein-Barr virus induced gene 3 (EBI3) and has anti-inflammatory properties that regulate T cell polarization and cytokine production. IL-27-deficient (Ldlr-/Ebi3-/-) and IL-27 receptor-deficient (Ldlr-/-WSX-1-/-) Ldlr-/- mice were generated and fed with a high-cholesterol diet to induce atherosclerosis. Roles of bone marrow-derived cells in vivo and macrophages in vitro were studied using bone marrow reconstitution by transplantation and cultured peritoneal macrophages, respectively. We demonstrate that mice lacking IL-27 or IL-27 receptor are more susceptible to atherosclerosis compared with wild type due to enhanced accumulation and activation of macrophages in arterial walls. The number of circulating proinflammatory Ly6Chi monocytes showed no significant difference between wild-type mice and mice lacking IL-27 or IL-27 receptor. Administration of IL-27 suppressed the development of atherosclerosis in vivo and macrophage activation in vitro that was indicated by increased uptake of modified low-density lipoprotein and augmented production of proinflammatory cytokines. These findings define a novel inhibitory role for IL-27 in atherosclerosis that regulates macrophage activation in mice.

    DOI: 10.1152/ajpheart.00198.2013

  • Anti-cancer effect of water extracts prepared from chrysanthemum flowers and leaves shiranui himekiku 査読

    Kanako Murayama, Tomoko Sawa, Hiroaki Koga, Satoshi Yamaguchi, Motofumi Kumazoe, Takafumi Otsuka, Yoshiyuki Miyazaki, Hirofumi Tachibana, Koji Yamada

    Nippon Shokuhin Kagaku Kogaku Kaishi   60 ( 2 )   80 - 86   2013年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    The flower of the chrysanthemum plant Shiranui Himekiku has been used as a folk medicine for a variety of diseases including cancer, and cytotoxic effects in some cancer lines have been reported. However, little is known about the anti-cancer activity of the leaf extract and its active compounds. Therefore, to clarify the anti-cancer activity of Shiranui Himekiku leaf and flower, the anti-proliferative effects of the extracts on human cancer cells were examined. Both leaf and flower water extracts dose-dependently suppressed the proliferation of the human breast cancer cell line MCF-7 at concentrations >25 μg/ml. Furthermore, the anti-proliferative effect in MCF-7 is more than four times that in normal human dermal fibroblast cells. When heated at 100- for 30 minutes, the proliferation inhibitory activity of the flower extract was unchanged. In contrast, under identical conditions, the activity of the leaf extract was lost. The extracts were separated into two fractions; one with a molecular weight (MW) >10 000 (high molecular weight fraction) (HMW) and the other with a MW>10 000 (low molecular weight fraction) (LMW). A significant proliferation inhibitory effect can be seen in the LMW fraction of both extracts, but the HMW fraction of the leaf extract also slightly inhibits cell growth. Both polyphenols and non-polyphenols are candidate active compounds because the anti-cancer effect was seen in the polyphenol-and non-polyphenol-fractions. These results suggest that the extracts contain several types of anti-cancer components and that both leaves and flowers can be useful.

    DOI: 10.3136/nskkk.60.80

  • Histamine release-suppressive effect of water extracts prepared from flower and leaf of chrysanthemum, Shiranui himekiku 査読

    Koji Yamada, Tomoko Sawa, Kanako Murayama, Satoshi Yamaguchi, Yoshiyuki Miyazaki, Hirofumi Tachibana

    Nippon Shokuhin Kagaku Kogaku Kaishi   59 ( 8 )   394 - 400   2012年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    To clarify the immunoregulatory activity of the chrysanthemum plant Shiranui himekiku, the effects of its flower and leaf extracts on proliferation and histamine release of rat and human basophilic leukemia cells were examined. Water extracts of flower and leaf suppressed proliferation and histamine release of rat RBL-2H3 cells dose-dependently at concentrations over 10μg/mL. The histamine release-suppressive effect of the leaf extract was as strong as that of the flower extract. The effects of these extracts on proliferation and histamine release of human KU812 cells were much weaker than those on RBL-2H3 cells. When these extracts were heated at 100°C for 30 min, the histamine release-inhibitory activity was unchanged, but the proliferation-inhibitory activity decreased slightly. Histamine release-inhibitory activity was detected in water extract of a commercial dried flower extract prepared with a stainless kettle as well as that prepared with an earthen pot as recommended by the manufacturer. These results suggest that these extracts contain anti-allergic components, that leaves are useful as well as flowers, and that the extracts can be prepared using stainless pots, which are useful in IH cooking.

    DOI: 10.3136/nskkk.59.394

  • IL-27 inhibits hyperglycemia and pancreatic islet inflammation induced by streptozotocin in mice 査読

    Hirokazu Fujimoto, Tetsuaki Hirase, Yoshiyuki Miyazaki, Hiromitsu Hara, Noriko Ide-Iwata, Ai Nishimoto-Hazuku, Christiaan J.M. Saris, Hiroki Yoshida, Koichi Node

    American Journal of Pathology   179 ( 5 )   2327 - 2336   2011年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Inflammation driven by immune cells and pro-inflammatory cytokines is implicated in pancreatic β-cell injury, leading to the development of diabetes mellitus. IL-27, a cytokine consisting of IL-27p28 and Epstein-Barr virusinduced gene 3 (EBI3), binds a membrane-bound heterodimeric receptor consisting of the IL-27 receptor α chain (WSX-1) and gp130. IL-27 has anti-inflammatory properties that regulate T-cell polarization and cytokine production. We evaluated blood glucose and islet proinsulin concentrations, inflammatory cell infiltration in islets, and expression of IL-1β mRNA in pancreas in wild-type (WT), EBI3 -/-, and WSX-1 -/- mice treated with streptozotocin (STZ). Hyperglycemia was augmented in EBI3 -/- and WSX-1 -/- mice compared with WT mice. Islet proinsulin levels after STZ treatment were lower in EBI3 -/- and WSX-1 -/- mice than in WT mice. The infiltration of islets by F4/80 +CD11c -7/4 - macrophages, CD4 + T cells, and CD8 + T cells was increased in EBI3 -/- and WSX-1 -/- mice compared with WT mice. The administration of recombinant IL-27, compared with control, decreased the blood glucose level, immune cell infiltration into islets, and IL-1β mRNA expression in the pancreas and increased islet proinsulin levels in WT and EBI3 -/- mice. Thus, IL-27 inhibits STZ-induced hyperglycemia and pancreatic islet inflammation in mice and represents a potential novel therapeutic approach for β-cell protection in diabetes.

    DOI: 10.1016/j.ajpath.2011.08.001

  • IL-17 is necessary for host protection against acute-phase Trypanosoma cruzi infection 査読

    Yoshiyuki Miyazaki, Shinjiro Hamano, Seng Wang, Yohei Shimanoe, Yoichiro Iwakura, Hiroki Yoshida

    Journal of Immunology   185 ( 2 )   1150 - 1157   2010年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    IL-17A is a key cytokine that induces inflammatory responses through the organized production of inflammatory cytokines, such as IL-6, TNF-α, and GM-CSF, and induces neutrophil migration. The roles of IL-17A in infection of intracellular protozoan parasites have not been elucidated, although augmented immune responses by IL-17A are important for the resolution of some bacterial and fungal infections. Therefore, we experimentally infected IL-17A-deficient (IL-17A-/-) mice with Trypanosoma cruzi. IL-17A-/- mice had a lower survival rate and prolonged worse parasitemia compared with control C57BL/6 wild-type (WT) mice postinfection. In the infected IL-17A-/- mice, multiple organ failure was observed compared with WT mice, as reflected by the marked increase in serologic markers of tissue injury, such as aspartate aminotransferase, which resulted in increased mortality of IL-17A-/- mice. Expression of cytokines, such as IFN-γ, IL-6, and TNF-α, was lower in liver-infiltrating cells from the IL-17A-/- mice compared with WT mice. A similar defect was observed in the expression of neutrophil enzymes, such as myeloperoxidase and lipoxygenase, whereas cellular infiltration into the infected tissues was not affected by IL-17A deficiency. These results suggested that the efficient activation of immune-related cells critical for the killing of T. cruzi was impaired in the absence of IL-17A, resulting in the greater susceptibility of those mice to T. cruzi infection. From these results, we conclude that IL-17A is important for the resolution of T. cruzi infection.

    DOI: 10.4049/jimmunol.0900047

  • Exacerbation of delayed-type hypersensitivity responses in EBV-induced gene-3 (EBI-3)-deficient mice 査読

    Honglian Tong, Yoshiyuki Miyazaki, Masanori Yamazaki, Hiromitsu Hara, Herman Waldmann, Shohei Hori, Hiroki Yoshida

    Immunology Letters   128 ( 2 )   108 - 115   2010年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Epstein-Barr virus-induced gene-3 (EBI-3) associates with p28 to form interleukin-27 (IL-27) or with IL-12p35 to form IL-35. Both IL-27 and IL-35 have immunosuppressive functions and especially IL-35 has been implicated in the suppressive function of regulatory T cells (Treg). To address the role of EBI-3 in immune regulation, delayed-type hypersensitivity (DTH) responses were examined in EBI-3-deficient (EBI-3-/-) mice. EBI-3-/- mice developed deteriorated DTH responses as shown by the enhanced footpad swelling and augmented infiltration of inflammatory cells into the antigen-challenged footpads as compared with wild-type (WT) mice. While EBI-3-deficiency showed little effects on antigen-specific IFN-γ production of lymph node cells, IL-17 production was drastically augmented in EBI-3-/- cells as compared with in WT cells. In addition, reduced IL-10 production was also evident in EBI-3-/- CD4+ T cells. Interestingly, the development and suppressive function of Treg to inhibit effector T cell proliferation was not affected by EBI-3-deficiency. These data clearly demonstrated the immunosuppressive function of EBI-3 and provided complementary evidence that EBI-3 and EBI-3-containing cytokines might be taken into consideration as potential targets for some immune-related diseases.

    DOI: 10.1016/j.imlet.2010.01.001

  • Interleukin 27 A double-edged sword for offense and defense 査読

    Hiroki Yoshida, Mako Nakaya, Yoshiyuki Miyazaki

    Journal of Leukocyte Biology   86 ( 6 )   1295 - 1303   2009年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Cytokine-mediated immunity plays a crucial role in the pathogenesis of various diseases including infection and autoimmune diseases. IL-27, along with IL-12, -23, and -35, belongs to the IL-12 cytokine family. These family members play roles in regulation of Th cell differentiation. IL-27 is unique in that although it induces Th1 differentiation, the same cytokine suppresses immune responses. In the absence of IL-27-mediated immunosuppression, hyperproduction of various proinflammatory cytokines concomitant with severe inflammation is observed. The immunosuppressive effects of IL-27 depend on IL-2 suppression, inhibition of Th17 development, and induction of IL-10 production. Administration of IL-27 suppresses some diseases of autoimmune or allergic origin, demonstrating its potential in therapy of diseases mediated by inflammatory cytokines. In this review, we discuss recent studies about the role of IL-27 in immune regulation in view of its pro- and antiinflammatory properties and possible therapeutic application.

    DOI: 10.1189/jlb.0609445

  • Tumor-specific cytotoxic T cell generation and dendritic cell function are differentially regulated by interleukin 27 during development of anti-tumor immunity 査読

    Yukari Shinozaki, Sen Wang, Yoshiyuki Miyazaki, Kohji Miyazaki, Hisakata Yamada, Yasunobu Yoshikai, Hiromitsu Hara, Hiroki Yoshida

    International Journal of Cancer   124 ( 6 )   1372 - 1378   2009年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Interleukin (IL-) 27 is a member of IL-12 cytokine family with Th1-promoting and anti-inflammatory effects, IL-27 has been shown to facilitate tumor-specific cytotoxic T lymphocyte (CTL) induction against various tumors. However, IL-27 suppresses cytokine production of lymphocytes and antigen-presenting function of dendritic cells (DCs). To examine the in vivo role of IL-27 in generation of anti-tumor immunity, we examined IL-27-mediated antitnmor-effects using WSX-1 (IL-27 receptor α chain)-deficient (WSX-1
    -/-
    ) mice. In WSX-1
    -/-
    mice inoculated with B16 melanoma cells, tumor growth was higher than in wild-type (WT) mice. Accordingly, tumor-specific CTL generation was lower in WSX-1
    -/-
    mice than in WT mice. CTL induction in WSX-1
    -/-
    mice was not restored by transfer of WT DCs pulsed with TRP2 peptide, indicating that IL-27 is directly required for generation of tumor-speeiic CTLs. However, when transferred into tumor-bearing mice, WSX-1
    -/-
    DCs pulsed with TRP2 peptide was more potent than WT DCs in tumor growth inhibition and generation of CTLs, indicating suppressive effects of IL-27 on DC function. Finally, the combination of WT CD8
    +
    T cells and KO DCs is more potent in generation of antigen-specific CTLs than any other combinations. Expression of perforin gene and percentages of tumor-specific CD8
    +
    T cells were also the highest in the combination of WT CD8
    +
    T cells and WSX-1
    -/-
    DCs, It was thus revealed that IL-27 promotes CTL generation while suppressing DC function during generation of tumor immunity. The combination of WT T cells and IL-27 signal-defective DCs may have therapeutic potential against tumors.

    DOI: 10.1002/ijc.24107

  • Amelioration of experimental allergic rhinitis with suppression of topical immune responses by lack of IL-27/WSX-1 signaling 査読

    Yohei Shimanoe, Yoshiyuki Miyazaki, Hiromitsu Hara, Akira Inokuchi, Hiroki Yoshida

    Annals of Allergy, Asthma and Immunology   102 ( 3 )   223 - 232   2009年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    BackgroundAllergic rhinitis is 1 of the most common atopic diseases with strong similarity to asthma. Interleukin (IL) 27 is an immunosuppressive cytokine, and lack of the IL-27 receptor (WSX-1) resulted in exacerbation of allergic airway hyperresponsiveness. Objective: To address the role of IL-27/WSX-1 in the rhinitis model compared with the asthma model. Methods: Wild-type or WSX-1 / female mice were immunized intraperitoneally 4 times with ovalbumin adsorbed to aluminum potassium sulfate at a 1-week interval. The sensitized mice were then challenged for 14 days with ovalbumin intranasally from days 22 to 35. Clinical scores, serum antigen specific IgE levels, and cytokine production in the nasal lavage fluid were examined. Cytokine and chemokine expression in the cervical lymph nodes, nasopharynx-associated lymphoid tissues, and nasal mucosa was also examined. Results: WSX-1/ mice developed augmented immune responses in the serum (IgE production), cervical lymph nodes (cytokine and chemokine expression), and nasopharynx-associated lymphoid tissues (cytokine and chemokine expression), whereas local responses, such as clinical scores and nasal lavage fluid cytokine production, were reduced in WSX-1/ mice. Expression of some chemokines was also reduced in the nasal mucosal tissues of WSX-1 / mice. Conclusion: In contrast to the immunosuppressive role observed in the asthma model, IL-27 WSX-1 topically plays an exacerbating role in the pathogenesis of allergic rhinitis, presumably through differential expression of chemokines.

    DOI: 10.1016/S1081-1206(10)60085-3

  • Regulation of immune responses by interleukin-27 (Immunological Reviews (2008) 226, (234-247)) 査読

    Hiroki Yoshida, Yoshiyuki Miyazaki

    Immunological Reviews   227 ( 1 )   2009年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1111/j.1600-065X.2008.00738.x

  • Regulation of immune responses by interleukin-27 査読

    Hiroki Yoshida, Yoshiyuki Miyazaki

    Immunological Reviews   226 ( 1 )   234 - 247   2008年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Cytokine-mediated immunity plays a crucial role in the pathogenesis of various diseases including autoimmunity. Recently, interleukin-27 (IL-27) was identified, which, along with IL-12, IL-23, and IL-35, belongs to the IL-12 cytokine family. These family members play roles in the regulation of T helper (Th) cell differentiation. IL-27 is unique in that while it induces Th1 differentiation, the same cytokine suppresses immune responses. In the absence of IL-27-mediated immunosuppression, hyper-production of various pro-inflammatory cytokines concomitant with severe inflammation in affected organs was observed in IL-27 receptor α chain (WSX-1)-deficient mice infected with Trypanosoma cruzi. Experimental allergic or inflammatory responses were also enhanced in WSX-1-deficient mice. The immunosuppressive effects of IL-27 depend on inhibition of the development of Th17 cells (a newly identified inflammatory T-helper population) and induction of IL-10 production. Moreover, administration of IL-27 or augmentation of IL-27 signaling suppresses some diseases of autoimmune or allergic origin, demonstrating its potential in therapy of diseases mediated by inflammatory cytokines. In this review, we discuss recent studies on the role of IL-27 in immunity to parasitic and bacterial infections as well as in allergy and autoimmunity in view of its pro- and anti-inflammatory properties.

    DOI: 10.1111/j.1600-065X.2008.00710.x

  • Amelioration of delayed-type hypersensitivity responses by IL-27 administration 査読

    Yoshiyuki Miyazaki, Yohei Shimanoe, Seng Wang, Hiroki Yoshida

    Biochemical and Biophysical Research Communications   373 ( 3 )   397 - 402   2008年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Interleukin (IL-) 27 is a member of the IL-12 cytokine family. Although recent analyses of WSX-1 (IL-27 receptor α chain)-deficient mice as well as in vitro studies using recombinant IL-27 revealed the immunosuppressive function of IL-27, in vivo role and therapeutic potential of IL-27 remain poorly elucidated. Here we investigated the effect of IL-27 administration on delayed-type hypersensitivity (DTH). While WSX-1-deficient mice showed higher DTH responses as shown by the degree of footpad swelling, administration of IL-27 significantly ameliorated the footpad swelling. Since the activation status of the draining lymph node cells were not affected by IL-27 deficiency, it was suggested that IL-27 affected the effector phase of the response. These results collectively indicate that IL-27 has a suppressive effect on activated T cells in the experimental model and also has a therapeutic potential for some diseases caused by immune disorder.

    DOI: 10.1016/j.bbrc.2008.06.038

  • Interleukin 27 signaling pathways in regulation of immune and autoimmune responses 査読

    Hiroki Yoshida, Yoshiyuki Miyazaki

    International Journal of Biochemistry and Cell Biology   40 ( 11 )   2379 - 2383   2008年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Cytokine-mediated immunity plays a crucial role in pathogenesis of various diseases including autoimmune disease. Recently, interleukin 27 was identified, which along with interleukin 23 belongs to the interleukin 12 cytokine family. Interleukin 27 is pivotal for the induction of T helper 1 responses. Recent studies, however, revealed that interleukin 27 has an immunosuppressive property. In interleukin 27 receptor-deficient mice, various pro-inflammatory cytokines were over produced resulting in excess of immune responses. The immunosuppressive effects of interleukin 27 depend on suppression of interleukin 2 production, inhibition of the development of T helper 17 cells (a newly identified inflammatogenic T helper population), and induction of interleukin 10 production. Activation of signal transducers and activators of transcription 1 and 3 is critical in the immunosuppressive function of interleukin 27. Interleukin 27 suppresses some diseases of autoimmune or allergic origin, demonstrating its promising potential in therapy of diseases mediated by inflammatory cytokines.

    DOI: 10.1016/j.biocel.2008.05.020

  • Differential roles for IFN-γ and IL-17 in experimental autoimmune uveoretinitis 査読

    Takeru Yoshimura, Kohei Sonoda, Yoshiyuki Miyazaki, Yoichiro Iwakura, Tatsuro Ishibashi, Akihiko Yoshimura, Hiroki Yoshida

    International Immunology   20 ( 2 )   209 - 214   2008年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    IL-17-producing CD4+ T cells, so called Th17 cells, constitute a newly identified inflammatogenic cell population, which is critically involved in some inflammatory diseases. To explore the role of Th17 cells in murine experimental autoimmune uveoretinitis (EAU), a model of human autoimmune uveitis where Th1 responses predominantly participate in the pathogenesis, IL-17- mice were immunized with interphotoreceptor retinoid-binding protein peptide 1-20 for disease induction. Funduscopic examination revealed that EAU was induced in IL-17- mice just like in wild-type (WT) mice at early phases of the disease. However, at later/maintenance phases, the severity was significantly reduced in IL-17- mice. Expression of IFN-γ and MCP-1 was comparable between WT and IL-17- mice during the time course. In vivo blockade of IFN-γ and IL-4 resulted in exacerbation of EAU at later phases with augmented IL-17 production. Taken together, our data demonstrated that IL-17/Th17 participates in the late phases of EAU and also that Th1 and Th17 responses are differentially required for EAU.

    DOI: 10.1093/intimm/dxm135

  • Augmentation of antigen-presenting and Th1-promoting functions of dendritic cells by WSX-1(IL-27R) deficiency 査読

    Sen Wang, Yoshiyuki Miyazaki, Yukari Shinozaki, Hiroki Yoshida

    Journal of Immunology   179 ( 10 )   6421 - 6428   2007年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    WSX-1 is the α subunit of the IL-27R complex expressed by T, B, NK/NKT cells, as well as macrophages and dendritic cells (DCs). Although it has been shown that IL-27 has both stimulatory and inhibitory effects on T cells, little is known on the role of IL-27/WSX-1 on DCs. LPS stimulation of splenic DCs in vivo resulted in prolonged CD80/CD86 expression on WSX-1-deficient DCs over wild-type DCs. Upon LPS stimulation in vitro, WSX-1-deficient DCs expressed Th1-promoting molecules higher than wild-type DCs. In an allogeneic MLR assay, WSX-1-deficient DCs were more potent than wild-type DCs in the induction of proliferation of and IFN-γ production by responder cell proliferation. When cocultured with purified NK cells, WSX-1-deficient DCs induced higher IFN-γ production and killing activity of NK cells than wild-type DCs. As such, Ag-pulsed WSX-1-deficient DCs induced Th1-biased strong immune responses over wild-type DCs when transferred in vivo. WSX-1-deficient DCs were hyperreactive to LPS stimulation as compared with wild-type DCs by cytokine production. IL-27 suppressed LPS-induced CD80/86 expression and cytokine production by DCs in vitro. Thus, our study demonstrated that IL-27/WSX-1 signaling potently down-regulates APC function and Th1-promoting function of DCs to modulate overall immune responses.

    DOI: 10.4049/jimmunol.179.10.6421

  • Regulation of defense responses against protozoan infection by interleukin-27 and related cytokines 査読

    Hiroki Yoshida, Yoshiyuki Miyazaki, Sen Wang, Shinjiro Hamano

    Journal of Biomedicine and Biotechnology   2007   2007年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Cytokine-mediated immunity is crucial in the defense against pathogens.Recently, IL-23 and IL-27 were identified, which along with IL-12 belong to the IL-12 cytokine family. IL-27 is pivotal for the induction of helper T cell (Th) 1 responses while IL-23 is important for the proliferation of memory type Th1 cells. Recent studies revealed that IL-27 also has an anti-inflammatory property. In some protozoan infection, various proinflammatory cytokines were over produced causing lethal inflammatory responses in IL-27 receptor-deficient mice. The anti-inflammatory effect of IL-27 depends, at least partly, on inhibition of the development of Th17cells, a newly identified Th population that is induced by IL-23 and is characterized by the production of the inflammatogenic cytokine, IL-17.IL-27 thus has a double identity as an initiator and as an attenuator of immune responses and inflammation. With the discoveries of the new IL-12-related cytokines and Th17 cells, Th development is facing a new paradigm.

    DOI: 10.1155/2007/79401

  • Two-sided roles of IL-27 Induction of Th1 differentiation on naive CD4+ T cells versus suppression of proinflammatory cytokine production including IL-23-induced IL-17 on activated CD4+ T cells partially through STAT3-dependent mechanism 査読

    Takeru Yoshimura, Atsunobu Takeda, Shinjiro Hamano, Yoshiyuki Miyazaki, Ichiko Kinjyo, Tatsuro Ishibashi, Akihiko Yoshimura, Hiroki Yoshida

    Journal of Immunology   177 ( 8 )   5377 - 5385   2006年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Recent lines of evidence have demonstrated that IL-27, a newly identified IL-12-related cytokine, has two apparently conflicting roles in immune responses: one as an initiator of Th1 responses and the other as an attenuator of inflammatory cytokine production. Although the BL-27-mediated Th1 initiation mechanism has been elucidated, little is known about the molecular basis for the suppression of cytokine production. In the present study, we demonstrated that IL-27 suppressed the production of various proinflammatory cytokines by fully activated CD4+ T cells while it had no effect on the cytokine production by CD4+ T cells at early phases of activation. IL-27 also suppressed IL-17 production by activated CD4+ T cells, thereby counteracting IL-23, another IL-12-related cytokine with proinflammatory effects. In fully activated CD4+ T cells, STAT3 was preferentially activated by IL-27 stimulation, whereas both STAT1 and 3 were activated by IL-27 in early activated CD4+ T cells. Lack of STAT3 in fully activated cells impaired the suppressive effects of IL-27. These data indicated that the preferential activation of STAT3 in fully activated CD4+ T cells plays an important role in the cytokine suppression by IL-27/WSX-1.

    DOI: 10.4049/jimmunol.177.8.5377

  • Generation and characterization of B7-H4/B7S1/B7x-deficient mice 査読

    Woong Kyung Suh, Seng Wang, Gordon S. Duncan, Yoshiyuki Miyazaki, Elizabeth Cates, Tina Walker, Beata U. Gajewska, Elissa Deenick, Wojciech Dawicki, Hitoshi Okada, Andrew Wakeham, Annick Itie, Tania H. Watts, Pamela S. Ohashi, Manel Jordana, Hiroki Yoshida, Tak W. Mak

    Molecular and Cellular Biology   26 ( 17 )   6403 - 6411   2006年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Members of the B7 family of cosignaling molecules regulate T-cell proliferation and effector functions by engaging cognate receptors on T cells. In vitro and in vivo blockade experiments indicated that B7-H4 (also known as B7S1 or B7x) inhibits proliferation, cytokine production, and cytotoxicity of T cells. B7-H4 binds to an unknown receptor(s) that is expressed on activated T cells. However, whether B7-H4 plays nonredundant immune regulatory roles in vivo has not been tested. We generated B7-H4-deficient mice to investigate the roles of B7-H4 during various immune reactions. Consistent with its inhibitory function in vitro, B7-H4-deficient mice mounted mildly augmented T-helper 1 (Th1) responses and displayed slightly lowered parasite burdens upon Leishmania major infection compared to the wild-type mice. However, the lack of B7-H4 did not affect hypersensitive inflammatory responses in the airway or skin that are induced by either Th1 or Th2 cells. Likewise, B7-H4-deficient mice developed normal cytotoxic T-lymphocyte reactions against viral infection. Thus, B7-H4 plays a negative regulatory role in vivo but the impact of B7-H4 deficiency is minimal. These results suggest that B7-H4 is one of multiple negative cosignaling molecules that collectively provide a fine-tuning mechanism for T-cell-mediated immune responses.

    DOI: 10.1128/MCB.00755-06

  • Membranous glomerulonephritis development with Th2-type immune deviations in MRL/lpr mice deficient for IL-27 receptor (WSX-1) 査読

    Sakiko Shimizu, Naonobu Sugiyama, Kohsuke Masutani, Atsushi Sadanaga, Yoshiyuki Miyazaki, Yasushi Inoue, Mitsuteru Akahoshi, Ritsuko Katafuchi, Hideki Hirakata, Mine Harada, Shinjiro Hamano, Hitoshi Nakashima, Hiroki Yoshida

    Journal of Immunology   175 ( 11 )   7185 - 7192   2005年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    MKL/lpr mice develop spontaneous glomerulonephritis that is essentially identical with diffuse proliferative glomerulonephritis (World Health Organization class IV) in human lupus nephritis, Lupus nephritis is one of the most serious complications of systemic lupus erythematosus. Diffuse proliferative glomerulonephritis is associated with autoimmune responses dominated by Th1 cells producing high levels of IFN-γ. The initial mounting of Th1 responses depends on the function of the WSX-1 gene, which encodes a subunit of the IL-27R with homology to IL-12R. In mice deficient for the WSX-1 gene, proper Th1 differentiation was impaired and abnormal Th2 skewing was observed during infection with some intracellular pathogens. Disruption of the WSX-1 gene dramatically changed the pathophysiology of glomerulonephritis developing in MRL/lpr mice, WSX-1-/- MRL/lpr mice developed disease resembling human membranous glomerulonephritis (World Health Organization class V) with a predominance of IgG1 in glomerular deposits, accompanied by increased IgG1 and IgE in the sera. T cells in WSX-1 -/- MKL/lpr mice displayed significantly reduced IFN-γ production along with elevated IL-4 expression. Loss of WSX-1 thus favors Th2-type autoimmune responses, suggesting that the Th1/Th2 balance may be a pivotal determinant of human lupus nephritis development.

    DOI: 10.4049/jimmunol.175.11.7185

  • Exacerbation of experimental allergic asthma by augmented Th2 responses in WSX-1-deficient mice 査読

    Yoshiyuki Miyazaki, Hiromasa Inoue, Mikiko Matsumura, Koichiro Matsumoto, Takako Nakano, Miyuki Tsuda, Shinjiro Hamano, Akihiko Yoshimura, Hiroki Yoshida

    Journal of Immunology   175 ( 4 )   2401 - 2407   2005年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    WSX-1 (IL-27R) is a class I cytokine receptor with homology to gp130 and IL-12 receptors and is typically expressed on CD4+ T lymphocytes. Although previous reports have clarified that IL-27/WSX-1 signaling plays critical roles in both Th1 differentiation and attenuation of cell activation and proinflammatory cytokine production during some bacterial or protozoan infections, little is known about the importance of WSX-1 in cytokine-mediated diseases of allergic origin. To this aim, we took advantage of WSX-1-deficient (WSX-1-/-) mice and induced experimental asthma, in which Th2 cytokines are central modulators of the pathology. OVA-challenged WSX-1 -/- mice showed marked enhancement of airway responsiveness with goblet cell hyperplasia, pulmonary eosinophil infiltration, and increased serum IgE levels compared with wild-type mice. Production of Th2 cytokines, which are largely responsible for the pathogenesis of asthma, was augmented in the lung or in the culture supernatants of peribronchial lymph node CD4+ T cells from WSX-1-/- mice compared with those from wild-type mice. Surprisingly, IFN-γ production was also enhanced in WSX-1-/- mice, albeit at a low concentration. The cytokine overproduction, thus, seems independent from the Th1-promoting property of WSX-1. These results demonstrated that IL-27/WSX-1 also plays an important role in the down-regulation of airway hyper-reactivity and lung inflammation during the development of allergic asthma through its suppressive effect on cytokine production.

    DOI: 10.4049/jimmunol.175.4.2401

  • Hyperproduction of Proinflammatory Cytokines by WSX-1-Deficient NKT Cells in Concanavalin A-Induced Hepatitis 査読

    Atsushi Yamanaka, Shinjiro Hamano, Yoshiyuki Miyazaki, Kazunari Ishii, Atsunobu Takeda, Tak W. Mak, Kunisuke Himeno, Akihiko Yoshimura, Hiroki Yoshida

    Journal of Immunology   172 ( 6 )   3590 - 3596   2004年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Administration of Con A induces liver injury that is considered to be an experimental model for human autoimmune or viral hepatitis, where immunopathology plays roles mediated by activated lymphocytes, especially NK1.1+ CD3+ NKT cells, and inflammatory cytokines, including IFN-γ and IL-4. In the present study we investigated the role of WSX-1, a component of IL-27R, in Con A-induced hepatitis by taking advantage of WSX-1 knockout mice. WSX-1-deficient mice were more susceptible to Con A treatment than wild-type mice, showing serum alanine aminotransferase elevation and massive necrosis in the liver. Although the development of NKT cells appeared normal in WSX-1 knockout mice, purified NKT cells from the knockout mice produced more IFN-γ and IL-4 than those from wild-type mice in response to stimulation with Con A both in vitro and in vivo. In addition, hyperproduction of proinflammatory cytokines, including IL-1, IL-6, and TNF-α, was observed in the knockout mice after Con A administration. These data revealed a novel role for WSX-1 as an inhibitory regulator of cytokine production and inflammation in Con A-induced hepatitis.

    DOI: 10.4049/jimmunol.172.6.3590

  • Estrogenic compounds suppressed interferon-gamma production in mouse splenocytes through direct cell-cell interaction 査読

    Mako Nakaya, Masao Yamasaki, Yoshiyuki Miyazaki, Hirofumi Tachibana, Koji Yamada

    In Vitro Cellular and Developmental Biology - Animal   39 ( 8-9 )   383 - 387   2003年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Here, we reported the effects of 17β-estradiol (E2), isoflavone genistein (Gen), and daidzein (Dai) on the production of interferon (IFN)-γ by splenocytes isolated from C57BL/6N mice. When mouse splenocytes were stimulated with lipopolysaccharide, E2, Gen, and Dai suppressed the production of IFN-γ. However, when only nonadherent cell populations of splenocytes were tested, none of these estrogenic compounds suppressed IFN-γ production. This result indicates that IFN-γ production by nonadherent cell populations of splenocytes treated with estrogens is regulated by adherent cell populations. Moreover, direct cell-cell interaction between both populations was necessary for suppression of IFN-γ production by nonadherent populations. In addition, E2 conjugated with bovine serum albumin inhibited IFN-γ production as well as E2. This result suggests that the plasma membrane-associated estrogen receptor plays a prominent role in this suppression mechanism.

  • WSX-1 is required for resistance to Trypanosoma cruzi infection by regulation of proinflammatory cytokine production 査読

    Shinjiro Hamano, Kunisuke Himeno, Yoshiyuki Miyazaki, Kazunari Ishii, Atsushi Yamanaka, Atsunobu Takeda, Manxin Zhang, Hajime Hisaeda, Tak W. Mak, Akihiko Yoshimura, Hiroki Yoshida

    Immunity   19 ( 5 )   657 - 667   2003年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    WSX-1 is a class I cytokine receptor with homology to the IL-12 receptors and is essential for resistance to Leishmania major infection. In the present study, we demonstrated that WSX-1 was also required for resistance to Trypanosoma cruzi. WSX-1-/- mice exhibited prolonged parasitemia, severe liver injury, and increased mortality over wild-type mice. WSX-1 -/- splenocytes produced enhanced levels of Th2 cytokines, which were responsible for the prolonged parasitemia. Massive necroinflammatory lesions were observed in the liver of infected WSX-1-/- mice, and IFN-γ that was overproduced in WSX-1-/- mice compared with wild-type mice was responsible for the lesions. In addition, vast amounts of various proinflammatory cytokines, including IL-6 and TNF-α, were produced by liver mononuclear cells in WSX-1-/- mice. Thus, during T. cruzi infection, WSX-1 suppresses liver injury by regulating production of proinflammatory cytokines, while controlling parasitemia by suppression of Th2 responses, demonstrating its novel role as an inhibitory regulator of cytokine production.

    DOI: 10.1016/S1074-7613(03)00298-X

  • Inhibitory effect of peroxisome proliferator-activated receptor-γ ligands on the expression of IgE heavy chain germline transcripts in the human B cell line DND39 査読

    Yoshiyuki Miyazaki, Hirofumi Tachibana, Koji Yamada

    Biochemical and Biophysical Research Communications   295 ( 2 )   547 - 552   2002年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    The expression of ε germline transcripts (εGT) induced by interleukin (IL)-4 stimulation is essential for the progression of IgE-directed class switching. In this study, we examined the effects of various ligands for their ability to bind to the peroxisome proliferator-activated receptors (PPARs) and to modify the IL-4-induced εGT expression in the human B cell line DND39. We show here that the PPARγ ligand, 15-deoxy-Δ12;14-prostaglandin J2 (15d-PGJ2), can suppress εGT expression at 1 μM without inhibiting cell proliferation. A synthetic and PPARγ-specific ligand, ciglitazone, also suppressed εGT expression in a dose-dependent manner at concentrations between 10 and 50μM. Agonists for other PPAR isoforms did not affect εGT expression at concentrations between 0.01 and 10μM. We also demonstrated that 1μM 15d-PGJ2 was able to suppress the IL-4-induced phosphorylation of the Signal Transducer and Activator of Transcription 6 (STAT6), which is a transcription factor essential for εGT expression. Therefore, the suppression of STAT6 phosphorylation by 15d-PGJ2 is thought to participate in the inhibition of εGT expression. These results suggest that PPARγ ligands inhibit IL-4-induced IgE class switching in B lymphocytes.

    DOI: 10.1016/S0006-291X(02)00709-X

  • Polyunsaturated fatty acids are not essential for survival and proliferation of a human B cell line [2] 査読

    Yoshiyuki Miyazaki, M. Noguchi, Hirofumi Tachibana, K. Yamada

    In Vitro Cellular and Developmental Biology - Animal   37 ( 7 )   399 - 401   2001年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1290/1071-2690(2001)037<0399:PFAANE>2.0.CO;2

  • Oxidative stress by visible light irradiation suppresses immunoglobulin production in mouse spleen lymphocytes 査読

    Yoshiyuki Miyazaki, Masao Yamasaki, Hiroko Mishima, Keiko Mansho, Hirofumi Tachibana, Koji Yamada

    Bioscience, Biotechnology and Biochemistry   65 ( 3 )   593 - 598   2001年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    In this study, we attempted to induce the oxidative stress in mouse spleen lymphocytes with visible light irradiation and examined the effects of lipid peroxidation on immunoglobulin (Ig) production. The spleen lymphocytes were isolated from 8-week-old male balb/c mice and irradiated with 300 W visible light. When the cells were cultured for 72 hr, Ig contents in culture supernatants were decreased gradually by irradiation for over 30 min. The cell viability was also lowered by the irradiation. Intracellular phosphatidylcholine hydroperoxide (PCOOH) levels and thiobarbituric acid-reactive substances (TBARS) values in culture supernatants were measured as indices of lipid peroxidation and we found that Ig production by mouse spleen lymphocytes was suppressed accompanied with the progress of peroxidation of intracellular phospholipids. Cell membrane fluidity was also significantly decreased, but the intracellular Ig level was not changed in the irradiated cells. These results suggest that the peroxidation of intracellular lipids is a cause of the suppression of Ig production by mouse spleen lymphocytes via lowering cell viability and suppressing Ig synthesis and secretion.

    DOI: 10.1271/bbb.65.593

  • Effect of dietary cabbage fermentation extract and young barley leaf powder on immune function of Sprague-Dawley rats 査読

    Yoshiyuki Miyazaki, Y. Tokunaga, K. Takagaki, S. Tsusaki, Hirofumi Tachibana, K. Yamada

    Journal of Nutritional Science and Vitaminology   47 ( 3 )   253 - 257   2001年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    We investigated dietary effects of cabbage fermentation extract (CFE) and young barley leaf powder (YBLP) on rat immune functions, Male Sprague-Dawley rats of 4 wk age were fed for 3 wk diets containing these samples at 0.1 or 1% level. After the feeding period, serum IgG level was significantly higher in the rats fed 1% CFE. IgG productivity of spleen lymphocytes was enhanced dose-dependently in both groups of CFE and YBLP. Furthermore, IgG productivity of mesenteric lymph node (MLN) lymphocytes was approximately 2 times higher in the rats fed 1% CFE diet than in the control ones. IgA productivity of MLN lymphocytes tended to increase in both of CFE and YBLP groups. From these results, it was suggested that dietary CFE and YBLP reinforced Ig productivity in both systemic and intestinal immune systems. Moreover, CFE feeding tended to enhance the production of TNF-α by spleen lymphocytes. In spleen phospholipids, the level of arachidonic acid, a substrate for inflammatory lipid mediators, was not affected by CFE or YBLP feeding.

    DOI: 10.3177/jnsv.47.253

  • Effect of dietary conjugated linoleic acid on the in vivo growth of rat hepatoma dRLh-84 査読

    Masao Yamasaki, Atsushi Ikeda, Akira Hirao, Yoko Tanaka, Yoshiyuki Miyazaki, Tatsuya Rikimaru, Mitsuo Shimada, Keizo Sugimachi, Hirofumi Tachibana, Koji Yamada

    Nutrition and Cancer   40 ( 2 )   140 - 148   2001年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    We examined the effect of dietary conjugated linoleic acid (CLA) on the growth of injected hepatoma dRLh-84 in Donryu rats. After experimental diets containing 0% or 2% CLA were given to male Donryu rats for 3 wk, dRLh-84 cells were injected into the left lobe of the hepatic capsule, and the experimental diet was continued. The cells formed a solid tumor ≥1 wk after the injection, and thereafter the tumor grew with feeding duration. In a morphological study, this tumor appeared to be a low-differentiated hepatoma, and there was no remarkable difference in the morphology of the tumor between 0% and 2% CLA groups. Tumor weight was significantly higher in the 2% CLA group than in the 0% CLA group throughout the feeding period after the injection. Serum glutamic-oxaloacetic transaminase and glutamic-pyruvic transaminase activities were significantly higher in 2% CLA-injected rats than in 0% CLA-injected rats at 3 wk after the injection. CLA upregulated acyl-CoA oxidase activity, especially 1 wk after the injection. However, dietary CLA did not activate carnitine palmitoyl transferase II, which is a rate-limiting enzyme in the mitochondrial β-oxidation pathway. Natural killer cell activity in the spleen tended to be higher in injected rats, but a significant effect of dietary CLA was not recognized. Serum interferon-γ and tumor necrosis factor-α levels were higher in injected than in sham rats. More-over, these levels were higher in 2% CLA groups than in the respective 0% CLA groups.

    DOI: 10.1207/S15327914NC402_10

  • Optimization of estrogenic activity detection method using human breast cancer MCF-7 cells 査読

    Dal Ho Han, Yoshiyuki Miyazaki, Hirofumi Tachibana, Koji Yamada

    Journal of the Faculty of Agriculture, Kyushu University   44 ( 3-4 )   339 - 348   2000年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    We tried to optimize the assay system using human breast cancer MCF-7 cells for detection of estrogenic activity of environmental estrogens. Since fetal bovine serum (FBS) contains various growth factors including estrogen, 100 ml of FBS was treated with 5 g of activated charcoal to remove estrogen. MCF-7 cells were cultured with RPMI 1640 medium supplemented with FBS or charcoal-treated FBS (cFBS), in the presence of 17β-estradiol or flavonoids. Then, the cells were trypsinized and cell number was counted using a Coulter Counter. When the cells were cultured in the medium containing 0 to 5% FBS, proliferation-stimulating activity of daidzein and genistein was detected most strongly in the medium containing 1% FBS. In the case cFBS-supplemented medium, the effect of flavonoids was detectable, in all cFBS concentrations. The proliferation-stimulating effect of daidzein and genistein was detectable after a 72-hr lag period. In the presence of 1% FBS or 1% cFBS, 17 β -estradiol enhanced proliferation of the cells at the concentrations between 10-11 and 10-8M, while daidzein and genistein enhanced it only at 10-8M. On the other hand, quercetin and luteolin exerted a proliferation-inhibiting activity against MCF-7 cells at the concentrations over 10-11M. These results indicate that estrogenic activity of flavonoids against MCF-7 cells was detectable in the medium supplemented with 1% FBS or 1 to 5% cFBS. Proliferation-inhibitory activity of quercetin and luteolin suggests that these compounds exert anti-estrogenic and anti-cancer activities.

  • Effect of Rose Bengal on Immunoglobulin Production by Mouse B Lymphoma, WEHI-279 Cells 査読

    Yuichiro Kuramoto, Yoshiyuki Miyazaki, Shinichiro Inoue, Hirotaka Haruta, Hirofumi Tachibana, Michihiro Sugano, Koji Yamada

    Journal of Agricultural and Food Chemistry   46 ( 12 )   5368 - 5372   1998年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    The effect of Rose Bengal (RB) on immunoglobulin (Ig) production by mouse B lymphoma WEHI-279 cells was examined. At 100 μM, RB increased IgE concentration in the culture medium but decreased the levels of IgG and IgM. To examine whether RB affects to the secretion of IgE from WEHI-279 cells or its synthesis, intracellular, and extracellular Ig levels were compared. The cytoplasmic IgE level in the cells treated with 100 μM RB was higher than in the cells cultured without RB. Reverse transcriptase-polymerase chain reaction (RT-PCR) experiment showed that the level of productive ∈ transcripts of the RB-added group was higher than that of the control but RB did not affect the germ-line ∈ transcripts. These results suggested that RB enhancement of IgE was due to the increase of the Ig synthesis by WEHI-279 cells and not to the increase of secretion activity of Ig from the cells nor to the enhancement of class-switching to IgE producing cells.

    DOI: 10.1021/jf9804793

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講演・口頭発表等

  • The anti-inflammatic activity of a sulfated polysaccharide Fucoidan in innate immune cells 国際会議

    Y Miyazaki, T Satoyama, @H Nakano, @S Takeuchi, @H Takeuchi, @D Tachikawa

    106th Annual Meeting of The American Association of Immunologists  2022年5月 

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    開催年月日: 2022年5月

    記述言語:英語   会議種別:口頭発表(一般)  

    Fucoidan is a series of natural sulfated polysaccharides derived from brown seaweeds and have reported to possess various biological activities such as anti-tumor, anti-virus, and immuno-modulatory effects. In previous study, we revealed that fucoidan derived from Cladosiphon okamuranus activated murine macrophage-like cell line RAW264 cooperatively with Zymosan, a Saccharomyces cerevisiae-derived β-glucan. On the other hand, several reports demonstrated that fucoidan inhibited lipopolysaccharide (LPS)-induced production of nitrogen oxide (NO) and inflammatory cytokines by RAW264 cells. Therefore, in this study, we investigated the potential of fucoidan derived from Undaria pinnatifida to regulate the excessive activation of RAW264 cells in response to overstimulation with pathogen components. RAW264 cells were inoculated at 20000 cells/well in 96-well culture plates, and after a recovery culture for 24 hr, the cells were treated with fucoidan with or without appropriate concentrations of each ligand for pattern recognition receptors (PRRs) including Toll-like receptors (TLRs). Whereas the production of NO was significantly enhanced with fucoidan alone, it was dose-dependently inhibited by fucoidan under stimulation with Pam3CSK4 (TLR1/TLR2 ligand), heat killed Listeria monocytogenes (HKLM, TLR2 ligand), LPS (TLR4 ligand), Pam2CGDPKHPKSF (FSL-1, TLR2/TLR6 ligand). These results suggested that fucoidan have beneficial ability to alleviate excessive inflammatory reaction during pathogenic infection and maintain suitable immune balance.

  • in vitro実験系を用いたフコイダンのヒト腸内細菌叢に対する効果の解析

    #大野波月, @中野勇人, @柴崎哲哉, 里山俊哉, 宮﨑義之, 中山二郎

    日本農芸化学会  2022年3月 

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    開催年月日: 2022年3月

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:Online   国名:日本国  

  • Anti-angiogenic effect of fucoidan-mix AG via improvement of tumor microenvironment in a mouse melanoma model

    宮﨑義之,#朴俊夏,@中野勇人,@竹内秀吾,@竹内秀晃, @立川大介

    第50回日本免疫学会  2021年12月 

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    開催年月日: 2021年12月

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:Online   国名:日本国  

    Fucoidan is a series of natural sulfated polysaccharides constitute viscous component of brown seaweeds. Previously, we reported that a mixed preparation consists of high-molecular weight fucoidans derived from Okinawa-mozuku and Mekabu (sporophyll of Wakame) and Agaricus blazei mycelium extract, which named fucoidan-mix AG, acted to reinforce anti-tumor immune functions. In this study, we evaluated dietary effects of fucoidan-mix AG on tumor angiogenesis with C57BL/6 mice subcutaneously implanted basement membrane matrix (GeltrexTM) enclosing 5x104 mitomycin C-treated B16 melanoma cells or 500 ng/mL vascular endothelial growth factor (VEGF), and 2% fucoidan-mix AG containing diet was fed for 7 days before and after the matrix injection. As the result, accumulation of hemoglobin (Hb) and CD31-positive cells in the B16- enclosed matrix was lower by the fucoidan-mix AG feeding, which indicated that fucoidan-mix AG was effective to suppress tumor angiogenesis. In contrast, the Hb contents in the VEGF- enclosed matrix were not affected by the fucoidan-mix AG intake. Because the VEGF gene expression in the B16-enclosed matrix was reduced in the fucoidan-mix AG-fed mice, it was guessed that fucoidan-mix AG did not inhibit angiogenic activity of VEGF but suppressed VEGF production. In this context, the matrix infiltration of CD206-positive macrophages, which known to produce VEGF, were declined in the fucoidan-mix AG-fed mice. Furthermore, fucoidan-mix AG decreased matrix accumulation of Treg, and delayed B16 tumor growth. In conclusion, fucoidan-mix AG was expected to be effective in inhibition of tumor angiogenesis by improving immunosuppressive tumor microenvironment.

  • がん性血管新生および腫瘍の形成に及ぼすフコイダン− アガリクスミックス(CUA-フコイダン)の摂食効果

    宮﨑義之,#朴俊夏,@中野勇人,@竹内秀吾,@竹内秀晃, @立川大介

    第68回日本食品科学工学会  2021年8月 

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    開催年月日: 2021年8月

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:Online   国名:日本国  

    【目的】 フコイダンは,L-フコースを主な構成糖とする褐藻類由来の天然硫酸 化多糖であり,抗がん作用や抗ウイルス作用などの生理活性が報告されている. 先の研究で我々は, オキナワモズクおよびメカブに由来する2種類のフコイダン とアガリクス菌糸体エキス末を配合したフコイダン-アガリクスミックス(通称: CUA-フコイダン)が,免疫増強作用を通してがん腫瘍形成を効果的に抑制する ことを明らかにしてきた.本研究では,新たな視点としてがん性血管新生に着目 し CUA-フコイダンの摂食効果とその作用機序を検証した. 【方法】 本試験では C57BL/6 マウス(n=6)を用い,マイトマイシン C 処理により 増殖を阻害した 5x104 個の B16 メラノーマ細胞または 500 ng/mL の血管内皮細 胞増殖因子(VEGF)を封入した基底膜マトリクス(GeltrexTM)をマウスの背部皮 下に注射した.マトリクス皮下注射の前後 7 日間にわたり CUA-フコイダンを 2% 重量添加した AIN-93G 準拠食を自由摂食させ,マトリクスゲル内での血管形成 (ヘモグロビン(Hb)蓄積,血管内皮細胞数)および免疫細胞浸潤を評価した. 【結果】 B16 細胞を封入したマトリクスゲルにおける Hb 蓄積量は,CUA-フコイ ダン摂取群で有意に低値を示し,CD31 陽性血管内皮細胞の割合もまた CUA- フコイダン摂取群で減少傾向が観察された.これらの結果から,CUA-フコイダ ンはがん性血管新生に対して抑制作用を有することが示された.一方,VEGF を封入したマトリクスゲルでの Hb 蓄積には CUA-フコイダン摂取の効果は観察 されず,VEGF の血管新生誘導作用には影響を及ぼさないことが示された.し かし,ゲル中の VEGF 遺伝子発現は CUA-フコイダン摂取群で低値を示してお り,血管新生抑制の作用機序の一つとして,腫瘍内で VEGF を産生する CD206 陽性マクロファージの浸潤が CUA-フコイダン摂取に伴い減少することが考えら れた.さらに,CUA-フコイダンが抗腫瘍免疫の抑制に働く制御性 T 細胞(Treg) の浸潤減少や B16 腫瘍の成長遅延に働く可能性が併せて示された.以上の結 果から,CUA-フコイダンは腫瘍内の免疫抑制的な環境を改善し,がん性血管 新生および腫瘍形成の効果的な阻害に寄与することが期待された.

  • Regulatory mechanism of fucoidan-mix AG in intestinal mucosal immune cells 国際会議

    Y Miyazaki, #J Bak, @H Nakano, @S Takeuchi, @H Takeuchi, @D Tachikawa

    105th Annual Meeting of The American Association of Immunologists  2021年5月 

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    開催年月日: 2021年5月

    記述言語:英語   会議種別:口頭発表(一般)  

    開催地:Online  

    Fucoidan is a series of natural sulfated polysaccharides contained in brown sea algae, and their beneficial physiological activities such as anti-tumor, anti-virus and immune regulatory effects have been reported. In previous studies, we proved that fucoidans derived from Cladosiphon okamuranus (C. okamuranus) and Undaria pinnatifida effectively improved anti-tumor immunity and tumor vaccine efficiency in combination with an Agaricus blazei mycelium extract. It is thought that the fucoidan-agaricus mix (named fucoidan-mix AG) stimulate innate immune cells to activate intestinal and mucosal immune reaction, but actual target immune tissues and cells by fucoidan have not been evidenced yet. In this study, we investigated action mechanisms of fucoidan in regulation of mucosal immune activities. At first, immune cells were isolated from Payer’s patches, mesenteric lymph node (MLN) and intestinal epithelia of Balb/c mice, then stained with fluorophore-labeled fucoidan. As the results, it was revealed that fucoidan directly interact with lymphocytes besides dendritic cells and macrophages existing in these intestinal immune tissues. Furthermore, immune cells from Payer’s patches and MLN stimulated with fucoidan from C. okamuranus and fucoidan-mix AG produced more TNF-α, IL-12 and Th1 cytokine IFN-γ. The production of an immunosuppressive cytokine IL-10 also was augmented in the fucoidan-treated immune cells, which suggesting that fucoidan balanced immune system to prevent occurrence of excessive reaction. In addition, it was newly proved that fucoidan directly stimulated T lymphocytes to induce enhanced IFN-γ production.

  • 腸内環境および免疫機能に及ぼすあまおう乳酸菌およびフコイダンの摂食効果

    #篠田あかり, #朴俊夏, @中野勇人, @柴崎哲哉, 宮﨑義之, 中山二郎

    日本農芸化学会  2021年3月 

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    開催年月日: 2021年3月

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:オンライン   国名:日本国  

  • Immune-improving activities of fucoidan, seaweed-derived natural sulfated polysaccharides 招待 国際会議

    Y Miyazaki

    2021 Health Food Society of Taiwan Annual Meeting & International Symposium on Health Food Development in the Post-Pandemic Era  2021年3月 

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    開催年月日: 2021年3月

    記述言語:英語   会議種別:口頭発表(一般)  

    開催地:Ondemand   国名:台湾  

    Fucoidan is one of intercellular viscous components cover surface of brown sea seaweeds and a series of natural sulfated polysaccharides. Peoples in Asian countries eat seaweeds willingly as nourishing healthy foods, and fucoidan have been reported to have beneficial physiological activities such as anti-tumor, anti-virus and immune regulatory effects. In this context, we have proved in mouse model experiments that fucoidans derived from Cladosiphon okamuranus (Okinawa-mozuku) and Undaria pinnatifida (Mekabu) in combination with an Agaricus blazei mycelium extract effectively improved anti-tumor immunity, and potentially support effective induction of anti-tumor immune function by vaccination with tumor antigen peptide. Furthermore, we conducted open-label trials with the fucoidan-agaricus mix (named fucoidan-mix AG), and revealed that its intake was effective to augment natural killer (NK) cell activities and salivary secretory immunoglobulin A (sIgA) production in humans. On the other hand, we evaluated the capacity of Cladoshiphon okamuranus-derived fucoidan to activate murine macrophage-like cell line RAW264, and investigated the functional relationship with a Saccharomyces cerevisiae-derived β-glucan. As the results, it was shown that the interaction of fucoidan on cell surface activated RAW264 cells to produce more nitric oxide and TNF-α, and the maximum augmentation was observed in combinational stimulation with fucoidan and β-glucan. The results suggested that fucoidan and β-glucan had a cooperative effect to reinforce macrophage function. In conclusion, these results indicate that the intake of fucoidan is useful in augmentation of intestinal and mucosal immune defense, and expects to contribute to the prevention of cancer and infectious diseases and support our healthy life.

  • Ligand-aided quantitative and selective NMR assay for sulfated saccharides in solution. 国際会議

    Juneha Bak, Yoshiyuki Miyazaki, Toshiro Matsui

    International symposium between National Taiwan University and Kyushu University  2019年12月 

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    開催年月日: 2020年6月

    記述言語:英語   会議種別:口頭発表(一般)  

    開催地:Fukuoka, Japan   国名:日本国  

  • Suppressive effects of fucoidan-agaricus mix (CUA-fucoidan) on angiogenesis and tumor growth in B16 melanoma-bearing mice 国際会議

    Y Miyazaki, #J Bak, @H Nakano, @S Takeuchi, @H Takeuchi, @D Tachikawa

    104th Annual Meeting of The American Association of Immunologists  2020年5月 

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    開催年月日: 2020年5月

    記述言語:英語   会議種別:口頭発表(一般)  

    開催地:現地開催中止  

  • An establishment of NMR-based analytical assay for sulfated saccharides in solution 国際会議

    Juneha Bak, Yoshiyuki Miyazaki, Toshiro Matsui

    ICoFF2019/ISNFF2019 KOBE  2019年12月 

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    開催年月日: 2019年12月

    記述言語:英語   会議種別:口頭発表(一般)  

    開催地:Kobe, Japan   国名:日本国  

  • 硫酸化多糖フコイダンによるマクロファージ活性化機構の解明

    祝原由莉,中溝公次,竹内秀吾,竹内秀晃, 立川大介, 宮﨑義之

    第65回日本食品科学工学会大会  2018年8月 

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    開催年月日: 2018年8月

    記述言語:日本語  

    開催地:東北大学(仙台)   国名:日本国  

  • Potentiating effects of high-molecular weight fucoidan-agaricus mix (CUA) feeding on tumor vaccination 国際会議

    Y Miyazaki, Y Iwaihara, M Nakamizo, S Takeuchi, H Takeuchi, D Tachikawa

    102nd Annual Meeting of The American Association of Immunologists  2018年5月 

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    開催年月日: 2018年5月

    記述言語:英語  

    開催地:Austin Convention Center(Austin, TX)  

  • The induction of macrophage activation by a sulfated polysaccharide fucoidan

    宮﨑義之, 祝原由莉, 中溝公次, 竹内秀伍, 竹内秀晃, 立川大介

    第46回日本免疫学会  2017年12月 

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    開催年月日: 2017年12月

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:仙台国際センター(仙台)   国名:日本国  

  • Augmentation of salivary IgA production in healthy subjects by fucoidan-agaricus mix ingestion 国際会議

    宮﨑 義之, 中溝 公次, 川原 和弘, 竹内 英明, 立川 大介, 山田 耕路

    The American Association of Immunologists  2016年5月 

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    開催年月日: 2016年5月

    記述言語:英語  

    開催地:Seattle  

    Fucoidan is a series of sulfated polysaccharides derived from brown algae, and have reported to have various biological activities. Previously, we demonstrated that fucoidan derived from Cladosiphon okamuranus and Undaria pinnatifida effectively augmented anti-tumor immunity in combination with Agaricus blazei mycelia extract. In this study, we evaluated the capacity of the fucoidan-agaricus mix (FAM) to enhance oral mucosal immune function. Fifteen healthy volunteers (mean age, 41.2 years old; range, 22-56 years old; 8 males, 7 females) ingested 4 capsules each containing 250 mg FAM powder every day for 12 weeks. As a result, the mean of secretory rates of salivary secretory immunoglobulin A (sIgA) tended to be increased by the FAM administration for 4 and 12 weeks as compared with the initial value. The enhanced salivary sIgA secretion was more distinctly observed in subject group whose initial values of salivary sIgA secretory rate were lower than the total average. Furthermore, the intake of FAM led to significant augmentation in the salivary sIgA secretion (by 1.3-fold) in the group of subjects under 40 years of age. On the other hand, concanavalin A-induced blastogenic response of peripheral blood mononuclear cells and serum IgA concentration was not elevated during this trial. Therefore, it was suggested that FAM stimulated functional maturation rather than expansion of B lymphocyte. The intake of FAM did not significantly affect NK cell activities. In addition, the safety of FAM consumption was confirmed because no abnormal findings were observed in general clinical tests. From the results, it was suggested that intake of FAM was useful in augmentation of oral mucosal immune defense via enhancing salivary sIgA production.

  • Pilot clinical study to evaluate the immune enhancing activity of fucoidan mix 国際会議

    宮﨑 義之, 中溝 公次, 桐野 智美, 山口 千仁, 立花 宏文, 山田 耕路

    第12回アジア栄養学会議  2015年5月 

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    開催年月日: 2015年5月

    記述言語:英語  

    開催地:横浜   国名:日本国  

    Objectives: Fucoidan, sulfated polysaccharides from brown algae, have reported to have immunomodulatory effects, however the validation in human use is not enough. In this study, we performed an open label trial to evaluate the capability of a fucoidan-containing healthy food (fucoidan mix) to enhance immune function. Materials & Methods: The fucoidan mix is a powdered food blending Okinawamozuku fucoidan, Mekabu fucoidan and Agaricus mycelium extract. Fifteen healthy volunteers (mean age, 41.2 years; range, 22-56 years; 8 male, 7 female) were ingested 4 capsules containing 250 mg of fucoidan mix every day for 12 weeks. Immunological parameters including salivary secretory immunoglobulin A (sIgA) secretory rates, concanavalin A-induced blastogenic responses and NK cell activities of peripheral blood mononuclear cells were measured at before and 4, 8, 12 weeks after the fucoidan mix ingestion. Results & Findings: First of all, the mean of secretory rates of salivary sIgA tended to be increased by the fucoidan mix administration for 4 and 12 weeks as compared with the initial value. The enhanced secretion of sIgA was more distinctly observed in subject group whose initial values on sIgA secretory rate were lower than the whole average. Furthermore, fucoidan mix significantly augmented the sIgA secretion (by 1.3-fold) in the group of subjects under 40 years of age. On the other hand, the lymphocyte blastogenic response was not reinforced during this trial, and it was suggested that fucoidan mix stimulated rather functional maturation than expansion of B lymphocyte. The intake of fucoidan mix did not significantly affect NK cell activities. In addition, the safety of fucoidan mix was confirmed, because no abnormal findings were observed in general clinical tests. Conclusion: From the results, it was suggested that oral administration of fucoidan mix was useful in augmentation of oral mucosal immune defense through enhancement of salivary sIgA production.

  • フコイダン-アガリクスミックス経口摂取による抗がん剤投与に伴う免疫抑制の緩和 〜マウスモデル試験による検証〜

    宮﨑 義之, 中溝 公次, 桐野 智美, 立川 大介, 立花 宏文, 山田 耕路

    日本食品科学工学会  2014年8月 

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    開催年月日: 2014年8月

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:福岡   国名:日本国  

    【目的】 フコイダンは、主に L-フコースから成る褐藻由来の硫酸化多糖類であ る。フコイダンは、抗がん作用や免疫増強作用などの様々な生理活性を有する ことが多く報告されているが、抗がん剤治療と併用することの有用性は十分に検 討されていない。本研究では、オキナワモズクおよびメカブ由来のフコイダン抽 出物にアガリクスエキス末を混合した調製品(Fucoidan-Agaricus Mix, FAM)を 用い、抗がん剤の副作用である免疫抑制に対する緩和効果を検討した。 【方法】 実験には、Balb/c 雄マウスを用い、抗がん剤 5-フルオロウラシル (5-FU)をマウス 1 頭あたり 1 mg、隔日5回皮下注射することで、抗がん剤治療 モデル試験系を構築した。比較対照として、同量のリン酸緩衝生理食塩水 (PBS)を皮下注射した。また、5-FU 投与および非投与マウスには、それぞれ、1 日あたり 0, 20 または 40 mg の FAM を含む餌を、5-FU 投与開始の 3 日前から 最終投与の 2 日後まで与えた。その後、採血ならびに脾臓を採取し、脾臓細胞 の増殖能、サイトカイン産生能および免疫細胞集団の割合などを評価した。 【結果】 始めに、抗がん剤投与の効果を検討するために血中ヘモグロビン量お よび脾臓重量を測定した結果、共に 5-FU を投与したマウスで有意な低下が観 察され、抗がん剤副作用による骨髄抑制および免疫抑制の評価に適したモデ ル実験系であることが示唆された。また、本モデルマウスでは、がん細胞の殺傷 に働く NK 細胞の活性が 5-FU 投与によって有意に低下したが、FAM 摂取によ って用量依存的に上昇し、40 mg FAM 摂取群で正常値まで回復した。また、 NK 細胞などの抗腫瘍免疫に関わる細胞の活性化を誘導するインターフェロン -γ(IFN-γ)についても、5-FU 投与による低下が、FAM 摂取によって顕著に軽減 された。さらに、免疫細胞の存在比をフローサイトメーターで測定した結果、 IFN-γ を産生する Th1 細胞や抗腫瘍免疫の亢進に働くマクロファージなどで、 FAM 摂取による 5-FU 細胞毒性の緩和が観察された。以上の結果から、FAM 摂取が、抗がん剤 5-FU の副作用の一つである抗腫瘍免疫機能の低下の緩和 に有効であることが示唆された。

  • Immune enhancing effects of fucoidan-agaricus mix under treatment of an immunosuppressive anti-cancer agent 国際会議

    宮﨑 義之, 中溝 公次, 柴崎 哲哉, 桐野 智美, 川原 和弘, 竹内 英明, 立花 宏文, 山田 耕路, 立川 大介

    The American Association of Immunologists  2014年5月 

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    開催年月日: 2014年5月

    記述言語:英語  

    開催地:Pittsburgh  

    Fucoidan is a series of sulfated polysaccharides derived from brown algae that mainly consist of L-fucose. Fucoidan have various biological activities, and our investigation with experimental animal models and a healthy human trial demonstrated fucoidan from Cladosiphon okamuranus and Undaria pinnatifida effectively augmented anti-tumor immunity in combination with agaricus mycelium extract. In this context, we have reported that feeding of the fucoidan-agaricus mix (FAM) protected mice from immunosuppressive effect of 5-fluorouracil (5-FU). In current study, we confirmed dose-dependent immunoprotective effects of the dietary FAM (at 20 and 40 mg/day) by observing recovery in spleen weight, NK cell activity and IFN-γ production in 5-FU-treated mice. Furthermore, we investigated in vitro effects of FAM against 5-FU on growth and function of primary cultured immune-related cells. As the results with thioglycollate-induced peritoneal exudates cells (PEC), 5-FU dose-dependently suppressed the growth whereas FAM exerted no effects. However, production of cytokines such as IL-12p40 and TNF-α in PEC were stimulated by FAM treatment even in the presence of 5-FU. In the case of bone marrow derived cells differentiated with GM-CSF and M-CSF, FAM markedly enhanced the growth and IL-12 production of these cells. From these results, it was suggested that FAM contributed to amelioration of decline in anti-tumor immune function by 5-FU treatment through activation of innate immune cells.

  • Protective effects of orally administered fucoidan mix on immunosuppression by an experimental cancer chemotherapy 国際会議

    宮﨑 義之, 中溝 公次, 柴崎 哲哉, 桐野 智美, 齋藤 ゆず, 川原 和弘, 立花 宏文, 山田 耕路, 立川 大介

    The American Association of Immunologists  2013年5月 

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    開催年月日: 2013年5月

    記述言語:英語  

    開催地:Honolulu  

    Fucoidan are sulfated polysaccharides derived from brown seaweeds that mainly consist of L-fucose. Peoples in Asian countries eat seaweeds willingly as healthy foods. In previous studies, we have demonstrated that fucoidan augment anti-tumor immunity in an experimental mouse model and in healthy human subjects. However, availability of fucoidan to protect immune system from hazardous side effects of cancer chemotherapy has not been verified enough. Therefore, we performed a mouse model experiment in which immunosuppression was induced by subcutaneous 5-fluorouracil (5-FU) injection (1 mg/mouse/day, 5 times on alternate days) and evaluated protective effects of orally administered fucoidan mix (40 mg/mouse/day). The immunosuppression by 5-FU s.c. was confirmed with the loss in spleen weight of 5-FU-injected mice. NK cell activity was lower in 5-FU-injected mice than sham-treated control mice, but the decline was recovered by the fucoidan mix feeding. The intake of fucoidan mix significantly alleviated the reduction in interferon (IFN)-gamma production of splenocytes from 5-FU-injected mice. Furthermore, flow cytometric analyses revealed that specific population such as Th1 and CD11b+ cells were supported by the fucoidan mix feeding even in the 5-FU-injected mice. These results suggested that oral administration of fucoidan mix is effective to maintain tumor immunity during immunosuppressive cancer chemotherapy.

  • Enhancement of NK cell activity and Th1 immunity in healthy subjects by orally administered fucoidan mix 国際会議

    宮﨑 義之, 中溝 公次, 辻 博光, 桐野 智美, 齋藤 ゆず, 川原 和弘, 立花 宏文, 山田 耕路, 藤井 信, 立川 大介

    The American Association of Immunologists  2012年5月 

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    開催年月日: 2012年5月

    記述言語:英語  

    開催地:Boston  

    Fucoidan is a sulfated polysaccharide derived from brown seaweeds which mainly consist of L-fucose. In Asian countries such as Japan, Korea and China, peoples eat seaweeds willingly as nourishing health foods. We previously showed that the tumor formation by transplanted Sarcoma180 cells was suppressed in mice fed 2% fucoidan mix containing diets, and NK cell activity was enhanced in the fucoidan-fed mice. The results suggest that the activated NK cells participated in elimination of the tumor. However, in human, immune enhancing activities of fucoidan have hardly been proved. Therefore, we performed an open-label trial in this study. Five healthy volunteers (47 ± 6 years old, 2 male and 3 female) took 2.5 g fucoidan mix per day for 30 days. There were no abnormal observations in the values of WBC, RBC, platelet count and blood profile. On the other hand, NK cell activity was augmented by 1.42 times in the average at end of the trial than the initial value. The ratio of IFN-gamma-producing (Th1) cells also increased to 114% for the initial value by the day 30 by the fucoidan mix intake, whereas IL-4-producing (Th2) cells decreased to 77%. As a result, the rise of Th1/Th2 ratio by 1.46 times in the average was observed with 4 subjects among 5 examinees. Furthermore, CD8+ cells tended to expand with the all subjects. These results suggest that intake of fucoidan is effective to augment anti-tumor immunity in human.

  • Biological functions and anti-cancer effect of fucoidan from brown algae 国際会議

    宮﨑 義之, 山﨑(宮本)有美, 立花 宏文, 山田 耕路

    International Society for Nutraceuticals & Functional Foods  2010年10月 

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    開催年月日: 2010年10月

    記述言語:英語   会議種別:口頭発表(一般)  

    開催地:Bali   国名:インドネシア共和国  

    Fucoidan is one of viscous components from brown seaweeds including Cladosiphon okamuranus TOKIDA (Okinawamozuku), Kjellmaniella crassifolia (Gagome) and Undaria pinnatifida (Mekabu), and is collective term for sulfated polysaccharides which mainly consist of L-fucose. At present, fucoidan is known to have several biological activities such as anti-pathogenic, anti-oxidant, and anti-inflammatory activities. In particular, we focus on the anti-tumor activity of fucoidan, and have revealed that Okinawamozuku fucoidan induced apoptotic cell death of a human breast cancer cell line, MCF-7, by activating intracellular signaling cascade through caspase-8, (-9) and -7. Other plausible mechanisms for anti-tumor action of fucoidan are inhibition of angiogenesis, and buildup of anti-tumor immunity. It is thought that fucoidan stimulate innate immune cells existing in Peyer’s patch to induce production of cytokines including IFN-γ and IL-12, and they activate effector cells such as natural killer cells. Further experiments will surely provide useful information for applying fucoidan to cancer therapies.

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MISC

  • 高分子CUAフコイダン摂取による健常人の唾液IgA産生増強

    宮﨑 義之

    Bio Industry   2021年4月

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    記述言語:日本語   掲載種別:記事・総説・解説・論説等(学術雑誌)  

    フコイダンは、褐藻類の”ぬめり”成分を構成する天然硫酸化多糖類である。免疫力の向上や抗ウイルス作用に関する基礎的な研究報告は多いものの、臨床学的な知見は未だ少ない。本稿では、唾液分泌型IgA(sIgA)を口腔内粘膜免疫の指標とする健常人を対象とした高分子フコイダン-アガリクスミックス(以下、CUAフコイダン)投与試験を紹介する。検討の結果、CUAフコイダンが粘膜免疫の増強に寄与する可能性が示され、口腔や上気道等における感染防御およびアレルギー疾患の病態緩和に貢献することが期待される。

  • いちご果実由来乳酸菌 Lactobacillus plantarum AMAOU を用いた食品素材の開発と機能性評価

    宮﨑 義之, 中溝 公次, 柴崎 哲哉

    食品と開発 2019年4月号 特集/食成分による腸内環境活性   2019年4月

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    記述言語:日本語  

  • フコイダン−アガリクスミックス摂取によるペプチドワクチン処置マウスにおけるがん抗原特異的免疫応答の効率的誘導 査読

    宮﨑 義之

    New Food Industry   2019年2月

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    記述言語:日本語  

  • 第III編 成分単離・同定, 作用機序解析 第1章 成分単離・同定 4. 多糖類

    宮﨑 義之

    機能性食品開発のための初期評価試験プロトコール集(CMC出版)   2017年6月

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    記述言語:日本語  

  • 海藻由来機能性多糖フコイダンの免疫増強作用 (特集 水産物由来成分の機能研究)

    宮﨑 義之

    Food style 21   2016年12月

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    記述言語:日本語   掲載種別:記事・総説・解説・論説等(学術雑誌)  

  • フコイダン含有食品の免疫増強作用および安全性に関する小規模臨床試験 〜口腔内粘膜免疫機能の改善について〜

    宮﨑 義之, 桐野 智美, 山口 千仁

    Food style 21   2014年12月

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    記述言語:日本語   掲載種別:機関テクニカルレポート,技術報告書,プレプリント等  

    フコイダンは、褐藻類のヌメリを構成する一連の硫酸 化多糖類である。多くの基礎研究において免疫増強作用 や抗アレルギー作用を有することが報告されているが、 臨床学的な知見はまだ少ないのが現状である。そこで、 口腔内粘膜免疫の指標として唾液中分泌型 IgA(sIgA) に 着 目 し 、1 5 名 の 健 常 被 検 者 を 対 象 と す る 投 与 前 後 比 較 試験を実施した。その結果、フコイダン含有食品である フコイダンミックス(250 mg x 4回/1日)を 4から 12 週 間 摂 取 す る こ と で 、s I g A 分 泌 速 度 が 事 前 検 査 値 と 比 較 して上昇する傾向が示された。また、このフコイダンミ ックス摂取による sIgA 分泌速度の上昇は、事前検査値 がその平均より低い集団においてより強く観察された。 さらに、40歳未満の被検者集団における sIgA分泌速度 は、フコイダンミックス摂取に伴って有意に上昇した。 また、本試験期間を通じて、身体的異常を示す所見は認 められず、当該食品の安全性が確認された。以上の結果から、フコイダンミックスが口腔内粘膜免疫の増強に寄 与する可能性が示され、口腔内や上気道の病原体感染の 予防およびアレルギー疾患の病態緩和への貢献が期待さ れた。

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産業財産権

特許権   出願件数: 1件   登録件数: 0件
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所属学協会

  • 日本乳酸菌学会

  • 日本農芸化学会

  • 日本分析化学会

  • 日本免疫学会

  • The American Association of Immunologists

  • 日本食品科学工学会

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委員歴

  • 日本食品科学工学会 西日本支部   評議員   国内

    2022年3月 - 2024年2月   

  • 日本農芸化学会 西日本支部   参与   国内

    2020年4月 - 2023年12月   

学術貢献活動

  • 会計

    食品科学工学会第68回大会  ( 福岡 ) 2021年8月

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    種別:大会・シンポジウム等 

  • 会計

    食品科学工学会第67回大会  ( 福岡 ) 2020年8月

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    種別:大会・シンポジウム等 

  • 学術論文等の審査

    役割:査読

    2020年

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    種別:査読等 

    外国語雑誌 査読論文数:7

    日本語雑誌 査読論文数:0

    国際会議録 査読論文数:0

    国内会議録 査読論文数:0

  • 学術論文等の審査

    役割:査読

    2019年

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    種別:査読等 

    外国語雑誌 査読論文数:11

    日本語雑誌 査読論文数:0

    国際会議録 査読論文数:0

    国内会議録 査読論文数:0

  • 学術論文等の審査

    役割:査読

    2018年

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    種別:査読等 

    外国語雑誌 査読論文数:4

    日本語雑誌 査読論文数:0

    国際会議録 査読論文数:0

    国内会議録 査読論文数:0

  • 学術論文等の審査

    役割:査読

    2017年

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    外国語雑誌 査読論文数:6

    日本語雑誌 査読論文数:0

    国際会議録 査読論文数:0

    国内会議録 査読論文数:0

  • 学術論文等の審査

    役割:査読

    2016年

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    外国語雑誌 査読論文数:3

    日本語雑誌 査読論文数:0

    国際会議録 査読論文数:0

    国内会議録 査読論文数:0

  • 学術論文等の審査

    役割:査読

    2015年

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    外国語雑誌 査読論文数:4

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  • 学術論文等の審査

    役割:査読

    2014年

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    外国語雑誌 査読論文数:4

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  • 学術論文等の審査

    役割:査読

    2013年

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    外国語雑誌 査読論文数:1

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共同研究・競争的資金等の研究課題

  • キャピラリー電気泳動法に基づく天然硫酸化多糖類の品質および機能評価

    研究課題/領域番号:21K05423  2021年 - 2023年

    日本学術振興会  科学研究費助成事業  基盤研究(C)

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    担当区分:研究代表者  資金種別:科研費

  • 農芸化学中小企業産学・産官連携研究

    2020年4月 - 2021年3月

    日本農芸化学会 

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    担当区分:連携研究者 

    「あまおう由来乳酸菌と沖縄もずくフコイダンの腸内フローラ改善効果」の検証
    マウス摂食試験により、あまおう乳酸菌およびオキナワモズクフコイダン摂取が腸内細菌叢および腸管免疫に与える影響を検討

  • DOSY-NMR法による硫酸化多糖分析と腸管免疫制御機構に関する 研究

    研究課題/領域番号:17K07819  2017年 - 2019年

    日本学術振興会  科学研究費助成事業  基盤研究(C)

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    担当区分:研究代表者  資金種別:科研費

教育活動概要

  • 特別開講科目(後期)の開講
    大学院学生の研究指導

担当授業科目

  • 食品免疫機能分析学特論

    2022年10月 - 2022年12月   秋学期

  • 食品免疫機能分析学特論

    2021年10月 - 2021年12月   秋学期

  • 機能性多糖分析学特論

    2020年10月 - 2020年12月   秋学期

  • 機能性多糖分析学特論

    2019年10月 - 2019年12月   秋学期

  • 機能性多糖分析学特論

    2018年10月 - 2018年12月   秋学期

  • 機能性多糖分析学特論

    2017年10月 - 2018年3月   後期

  • 機能性多糖分析学特論

    2016年10月 - 2017年3月   後期

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FD参加状況

  • 2021年11月   役割:参加   名称:農学研究院FD「人を対象とする生命科学・医学系研究に関する倫理指針」について

    主催組織:部局

  • 2021年7月   役割:参加   名称:農学研究院FD「科研費を獲りにいこう! 科研費獲得の技術と工夫」

    主催組織:部局

  • 2020年9月   役割:参加   名称:科研費を獲りにいこう! 勝ち抜く気合と技術

    主催組織:部局

  • 2019年6月   役割:参加   名称:令和元年度 第1回農学研究院FD 「優良な博士人材の獲得と育成に向けて ~農学研究院教授に学ぶ ~」

    主催組織:部局

  • 2016年8月   役割:参加   名称:平成28年度安全衛生セミナー I(リスクアセスメントの導入について)

    主催組織:部局

  • 2016年7月   役割:参加   名称:平成28年度第1回農学研究院FD(本学における障害者支援について)

    主催組織:部局

  • 2016年4月   役割:参加   名称:平成28年度第1回全学FD(新任教員の研修)

    主催組織:全学

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社会貢献活動

  • 第2回市民講座  「健康寿命を延ばすための食生活」

    九州大学大学院農学研究院食品免疫機能分析学寄附講座  R4/6/1  九州大学 西新プラザ  2022年6月

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    対象:社会人・一般, 学術団体, 企業, 市民団体, 行政機関

    種別:講演会

    第1部:「健康を維持するためのサプリメントの上手な利用法」 食品免疫機能分析学寄附講座 准教授 宮﨑義之
    第2部:「農産物の有する機能性と味 ~苦味を制する者は健康を制する?~」  高知大学 理事・副学長 受田浩之氏

  • 第1回市民講座  「野菜で健康に!」

    九州大学大学院農学研究院食品免疫機能分析学寄附講座  R3/12/14 九州大学 西新プラザ  2021年12月

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    対象:社会人・一般, 学術団体, 企業, 市民団体, 行政機関

    種別:講演会

    第1部「食べると免疫力アップにつながる海の野菜(海藻)のお話」 九州大学農学研究院 准教授 宮﨑義之
    第2部「Dr.ベジフルの健康になるための野菜の力」髙上青果有限会社 代表取締役 髙上実氏

  • 「海藻多糖類の機能性 〜免疫賦活作用と抗ウイルス作用〜」  第31回食品産業創造展 西日本機能性食品開発研究会セミナー   主催:日刊工業新聞社 企画主催:日本食品科学工学会西日本支部 西日本機能性食品開発研究会   R3/5/19 マリンメッセ福岡(福岡)

    2021年

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    「海藻多糖類の機能性 〜免疫賦活作用と抗ウイルス作用〜」  第31回食品産業創造展 西日本機能性食品開発研究会セミナー   主催:日刊工業新聞社 企画主催:日本食品科学工学会西日本支部 西日本機能性食品開発研究会   R3/5/19 マリンメッセ福岡(福岡)

  • 食品保健指導士会 2020年度支部事業計画第1回受託セミナー 「コロナ対策 ̶ 免疫力UPの成分! フコイダンを中心に!」

    公益財団法人日本健康・栄養食品協会  オンライン講演  2020年11月

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    対象:社会人・一般, 学術団体, 企業, 市民団体, 行政機関

    種別:講演会

  • 第49期食品保健指導士養成講習会 「免疫と健康食品の活用」

    公益財団法人日本健康・栄養食品協会  R/1/7/28 パピヨン24(福岡)  2019年7月

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    対象:社会人・一般, 学術団体, 企業, 市民団体, 行政機関

    種別:その他

  • 健康フォーラム 〜免疫力の仕組みを理解して健康寿命を延ばそう〜 主催:株式会社アミー製薬 協賛:株式会社ヴェントゥーノ 後援:毎日新聞社営業総本部 朝日新聞社メディアビジネス局 R1/8/20 エルガーラホール・7 階多目的ホール(福岡)

    2019年

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    健康フォーラム 〜免疫力の仕組みを理解して健康寿命を延ばそう〜
    主催:株式会社アミー製薬 協賛:株式会社ヴェントゥーノ 後援:毎日新聞社営業総本部 朝日新聞社メディアビジネス局 R1/8/20 エルガーラホール・7 階多目的ホール(福岡)

  • 第47期食品保健指導士養成講習会 「免疫と健康食品の活用」

    公益財団法人日本健康・栄養食品協会  H30/7/30 パピヨン24(福岡)  2018年7月

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    対象:社会人・一般, 学術団体, 企業, 市民団体, 行政機関

    種別:その他

  • 「フコイダンとは? その規格認定について」   第28回食品産業創造展 機能性食品特別セミナー   主催:日刊工業新聞社 企画主催:日本食品科学工学会西日本支部 西日本機能性食品開発研究会   H30/5/23-25 マリンメッセ福岡(福岡)

    2018年

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    「フコイダンとは? その規格認定について」   第28回食品産業創造展 機能性食品特別セミナー   主催:日刊工業新聞社 企画主催:日本食品科学工学会西日本支部 西日本機能性食品開発研究会   H30/5/23-25 マリンメッセ福岡(福岡)

  • 西日本新聞健康フォーラム 〜免疫力の仕組みを理解して健康寿命を延ばそう〜   主催:西日本新聞社 協賛:(株)ヴェントゥーノ   H30/4/3 エルガーラホール・8階大ホール(福岡)

    2018年

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    西日本新聞健康フォーラム 〜免疫力の仕組みを理解して健康寿命を延ばそう〜   主催:西日本新聞社 協賛:(株)ヴェントゥーノ   H30/4/3 エルガーラホール・8階大ホール(福岡)

  • 「健康寿命を延ばす身体づくり」西日本新聞健康セミナー 〜免疫の仕組みを理解して健康な毎日を〜 主催:西日本新聞社 協賛:(株)ヴェントゥーノ H29/12/5 エルガーラホール・中ホール(福岡)

    2017年

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    「健康寿命を延ばす身体づくり」西日本新聞健康セミナー 〜免疫の仕組みを理解して健康な毎日を〜
    主催:西日本新聞社 協賛:(株)ヴェントゥーノ H29/12/5 エルガーラホール・中ホール(福岡)

  • 「天然多糖類と健康」第 27 回食品産業創造展 機能性食品特別セミナー 主催:日刊工業新聞社 企画主催:日本食品科学工学会西日本支部 西日本機能性食品開発研究会 H29/5/17-19 マリンメッセ福岡(福岡)

    2017年

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    「天然多糖類と健康」第 27 回食品産業創造展 機能性食品特別セミナー
    主催:日刊工業新聞社 企画主催:日本食品科学工学会西日本支部 西日本機能性食品開発研究会
    H29/5/17-19 マリンメッセ福岡(福岡)

  • 「認定健康食品(JHFA)の最新動向 〜新規格フコイダン食品について〜」 日本食品保健指導士会九州支部研修会 H29/7/29 パピヨン24(福岡)

    2017年

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    「認定健康食品(JHFA)の最新動向 〜新規格フコイダン食品について〜」
    日本食品保健指導士会九州支部研修会 H29/7/29 パピヨン24(福岡)

  • NPOフコイダン研究所主催のセミナーにて講演

    2016年

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    NPOフコイダン研究所主催のセミナーにて講演

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メディア報道

  • もずくの効能を腸管免疫と腸内環境の改善の観点から健康効果を紹介 新聞・雑誌

    マキノ出版 月刊誌『ゆほびか』  2021年5月

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    もずくの効能を腸管免疫と腸内環境の改善の観点から健康効果を紹介

  • 「FUCOIDAN: Anti-Cancer Functions + Inhibitor of Covid-19?」

    Journal of Modern Healing  2021年3月

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    「FUCOIDAN: Anti-Cancer Functions + Inhibitor of Covid-19?」

  • 九州大学におけるオキナワモズク由来フコイダンの機能性研究紹介 新聞・雑誌

    日刊みなと新聞  2020年4月

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    九州大学におけるオキナワモズク由来フコイダンの機能性研究紹介

  • メディカルフロンティア「Japanese Super Foods ニッポン長寿食の秘密」 テレビ・ラジオ番組

    NHK BSプレミアム  2017年11月

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    メディカルフロンティア「Japanese Super Foods ニッポン長寿食の秘密」

  • メディカルフロンティア「Japanese Super Foods ニッポン長寿食の秘密」 テレビ・ラジオ番組

    NHK総合沖縄地域放送  2017年11月

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    メディカルフロンティア「Japanese Super Foods ニッポン長寿食の秘密」

  • メディカルフロンティア「Japanese Super Foods ニッポン長寿食の秘密」 テレビ・ラジオ番組

    NHK world  2017年9月

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    メディカルフロンティア「Japanese Super Foods ニッポン長寿食の秘密」

  • フコイダンの機能性紹介 新聞・雑誌

    読売新聞  2017年3月

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    フコイダンの機能性紹介

  • 機能性多糖分析学寄附講座開設の紹介 新聞・雑誌

    産経新聞  2016年6月

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    機能性多糖分析学寄附講座開設の紹介

  • フコイダンの機能性に関する解説 機能性多糖分析学寄附講座開設の紹介 新聞・雑誌

    西日本新聞  2016年4月

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    フコイダンの機能性に関する解説
    機能性多糖分析学寄附講座開設の紹介

  • 機能性多糖分析学寄附講座開設の紹介 新聞・雑誌

    朝日新聞  2016年4月

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    機能性多糖分析学寄附講座開設の紹介

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