Authorship：Last author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)  

Bacterial symbionts can promote insecticide resistance in their hosts by isolating and degrading insecticidal compounds or altering the expression of host genes. Although Wolbachia, a common endosymbiont in arthropods, typically does not influence insecticide resistance, there are cases of increased or decreased susceptibility. Due to the restrictions of applying conventional insecticides in a stored product setting, studies on alternative control methods are needed, including those on entomotoxic nanoparticles and the potential for resistance. For pests of stored beans, selenium nanoparticles (SeNPs) are relatively innocuous to the azuki bean beetle, Callosobruchus chinensis (L.) (Coleoptera: Chrysomelidae: Bruchinae). Here, we hypothesized that this response is mediated by Wolbachia, and we tested this using an isofemale line of C. chinensis (infected or uninfected with Wolbachia). Our results showed that the lifespan of Wolbachia-infected females was not affected by SeNPs, but increasing concentrations of SeNPs still had a negative effect on fecundity; in uninfected females, increasing concentrations of SeNPs significantly decreased both lifespan and fecundity. However, in males, SeNPs enhanced lifespan and decreased the incidence of sexual harassment behavior regardless of infection status (for uninfected males, the duration of harassment behavior also decreased). In the presence of males, 72-h female reproduction increased independent of infection status or SeNP treatment, but egg hatchability was reduced by male presence and SeNPs. This study documents a valuable example of symbiont-mediated resistance to entomotoxic nanoparticles.

Repository Public URL： https://hdl.handle.net/2324/7179440

Authorship：Last author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1038/s41598-023-35697-1

Repository Public URL： https://hdl.handle.net/2324/7179438

Authorship：Last author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)  

DOI： https://doi.org/10.1038/s41598-021-02713-1

Repository Public URL： https://hdl.handle.net/2324/7179449

Authorship：Corresponding author   Language：English   Publishing type：Research paper (scientific journal)  

DOI： https://doi.org/10.1007/s10526-021-10081-y

Repository Public URL： https://hdl.handle.net/2324/7179457

Authorship：Corresponding author   Language：English   Publishing type：Research paper (scientific journal)  

The agricultural use of silica (SiO2) nanoparticles (NPs) has the potential to control insect pests while the safety and tritrophic effects on plants and beneficial natural enemies remains unknown. Here, we evaluate the effects of silica NPs on insect pests with different feeding niches, natural enemies, and a plant. Silica NPs were applied at different concentrations (75–425 mg/L) on field-cultivated faba bean and soybean for two growing seasons. The faba bean pests, the cowpea aphid Aphis craccivora and the American serpentine leafminer Liriomyza trifolii, and the soybean pest, the cotton leafworm Spodoptera littoralis, were monitored along with their associated predators. Additional laboratory experiments were performed to test the effects of silica NPs on the growth of faba bean seedlings and to determine whether the rove beetle Paederus fuscipes is attracted to cotton leafworm-infested soybean treated with silica NPs. In the field experiments, silica NPs reduced the populations of all three insect pests and their associated predators, including rove beetles, as the concentration of silica NPs increased. In soybean fields, however, the total number of predators initially increased after applying the lowest concentration. An olfactometer-based choice test found that rove beetles were more likely to move towards an herbivore-infested plant treated with silica NPs than to a water-treated control, suggesting that silica NPs enhance the attraction of natural enemies via herbivore-induced plant volatiles. In the laboratory, while silica NPs inhibited the development of faba bean roots at 400 mg/L, they did not affect germination and shoot length.

DOI： https://doi.org/10.1038/s41598-021-93518-9

Repository Public URL： https://hdl.handle.net/2324/7179445

Authorship：Lead author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)  

The alfalfa weevil Hypera postica, native to the Western Palearctic, is an invasive legume pest with two divergent mitochondrial clades in its invading regions, the Western clade and the Eastern/Egyptian clade. However, knowledge regarding the native populations is limited. The Western clade is infected with the endosymbiotic bacteria Wolbachia that cause cytoplasmic incompatibility in host weevils. Our aim was to elucidate the spatial genetic structure of this insect and the effect of Wolbachia on its population diversity. We analyzed two mitochondrial and two nuclear genes of the weevil from its native ranges. The Western clade was distributed in western/central Europe, whereas the Eastern/Egyptian clade was distributed from the Mediterranean basin to central Asia. Intermediate mitotypes were found from the Balkans to central Asia. Most Western clade individuals in western Europe were infected with an identical Wolbachia strain. Mitochondrial genetic diversity of the infected individuals was minimal. The infected clades demonstrated a higher nonsynonymous/synonymous substitution rate ratio than the uninfected clades, suggesting a higher fixation of nonsynonymous mutations due to a selective sweep by Wolbachia. Trans-Mediterranean and within-European dispersal routes were supported. We suggest that the ancestral populations diversified by geographic isolation due to glaciations and that the diversity was reduced in the west by a recent Wolbachia-driven sweep(s). The intermediate clade exhibited a body size and host plant that differed from the other clades. Pros and cons of the possible use of infected-clade males to control uninfected populations are discussed.

DOI： https://doi.org/10.1038/s41598-021-88770-y

Repository Public URL： https://hdl.handle.net/2324/7179443

Authorship：Corresponding author   Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1111/een.12909

Authorship：Lead author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)  

Classification of specimens is the important first step to characterize populations and species assemblages. Although species-level classification has been a popular goal, the sex difference and sex ratio are also an important property in ecology and pest control. Here we focus on the images of mixed sex specimens of a stored product pest beetle (Callosobruchus chinensis) and its parasitoids (parasitic wasps; Anisopteromalus and Heterospilus) in various postures and classify them into species and sex, by training supervised machine learning programs: logistic model trees (LMT), random forest, support vector machine (SVM), simple logistic regression, multilayer perceptron and AdaBoost (adaptive boosting). Both object-based features and pixel-based features were extracted from each image. Simple logistic regression, LMT and AdaBoost (employing simple logistic regression as base learner) performed well to classify sexes or species/sexes; average true positive rates (prediction accuracy) of 88.5–98.5% were achieved for within-species sexing of beetles or wasps, 97.3% for two species sexing and 93.3% for three species sexing. For most datasets, the best performed models incorporated both object-based features and pixel-based features. LMT models were identical to simple logistic regression models in most cases. Robust performance and small variation in prediction accuracy of simple logistic regression, irrespective of classification target (sexes or species), was shown, and this is probably because of the efficient feature selection implemented in the algorithm. This study is one of the earliest to classify the gender of insects using machine learning based on still images.

DOI： 10.1016/j.ecoinf.2020.101135

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1002/ece3.5474

Language：English   Publishing type：Research paper (scientific journal)  

Aim: The seed beetle Callosobruchus maculatus is an important tropical and subtropical pest of legumes distributed world-wide. Archaeological evidence suggests an African origin with later world-wide invasion facilitated by the last centuries’ legume trading and exchange. To date, no studies could identify the routes or timing of dispersal of the species. Here, we investigate the global phylogeography of this pest to shed light on the main inter-continental dispersal routes that led to it becoming a cosmopolitan pest.
Location: World-wide.
Methods: We sampled seed beetles over a large fraction of the species’ range and sequenced one nuclear and three mitochondrial loci. Using this data, we estimated spatio-temporal phylogeographical reconstructions, and the demographic history of the species. We also used our dataset to evaluate the effect of panmixia on Bayesian demographic estimations.
Results: Callosobruchus maculatus exhibited regional and continental genetic structure, with the highest genetic diversity found in Africa. Our discrete Bayesian phylogeographical approach indicated that the species first dispersed to Asia and then colonized the pantropical belt. The three methods used for inferring the demographic history of C. maculatus indicated a recent demographic expansion in the world-wide dataset, as well as in the subset restricted to African samples. Such a signal was, however, not observed in the subset composed of Asian specimens. This demographic expansion occurred in the Holocene and is likely explained by the spread of cowpea and other host legumes across and out of Africa.
Main conclusions: The inferred dispersal routes support the idea that the evolutionary history of C. maculatus relates to the trade of its main host plant, Vigna unguiculata. Human-mediated processes appear to have shaped the global genetic structure of this pest. As a methodological implication, we demonstrate that coalescent-based demographic reconstructions can be erroneous if the dataset violates the assumption of panmixia.

DOI： 10.1111/jbi.13052

Language：English   Publishing type：Research paper (scientific journal)  

Maternally inherited bacterial endosymbionts in arthropods manipulate host reproduction to increase the fitness of infected females. Cytoplasmic incompatibility (CI) is one such manipulation, in which uninfected females produce few or no offspring when they mate with infected males. To date, two bacterial endosymbionts, Wolbachia and Cardinium, have been reported as CI inducers. Only Wolbachia induces complete CI, which causes 100% offspring mortality in incompatible crosses. Here we report a third CI inducer that belongs to a unique clade of Alphaproteobacteria detected within the coconut beetle, Brontispa longissima. This beetle comprises two cryptic species, the Asian clade and the Pacific clade, which show incompatibility in hybrid crosses. Different bacterial endosymbionts, a unique clade of Alphaproteobacteria in the Pacific clade and Wolbachia in the Asian clade, induced bidirectional CI between hosts. The former induced complete CI (100% mortality), whereas the latter induced partial CI (70% mortality). Illumina MiSeq sequencing and denaturing gradient gel electrophoresis patterns showed that the predominant bacterium detected in the Pacific clade of B. longissima was this unique clade of Alphaproteobacteria alone, indicating that this endosymbiont was responsible for the complete CI. Sex distortion did not occur in any of the tested crosses.
The 1,160 bp of 16S rRNA gene sequence obtained for this endosymbiont had only 89.3% identity with that of Wolbachia, indicating that it can be recognized as a distinct species. We discuss the potential use of this bacterium as a biological control agent.

DOI： 10.1073/pnas.1618094114

Authorship：Corresponding author   Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1007/s13355-016-0441-4

Authorship：Corresponding author   Language：English   Publishing type：Research paper (scientific journal)  

Bathyplectes anurus, a parasitoid of the alfalfa weevils, forms a cocoon in the late larval stage and exhibits jumping behaviour. Adaptive significance and costs of the cocoon jumping have not been thoroughly studied. We hypothesised that jumping has the fitness benefits of enabling habitat selection by avoiding unfavourable environments. We conducted laboratory experiments, which demonstrated that jumping frequencies increased in the presence of light, with greater magnitudes of temperature increase and at lower relative humidity. In addition, when B. anurus individuals were allowed to freely jump in an arena with a light gradient, more cocoons were found in the shady area, suggesting microhabitat selection. In a field experiment, mortality of cocoons placed in the sun was significantly higher than for cocoons placed in the shade. B. anurus cocoons respond to environmental stress by jumping, resulting in habitat selection. In the presence of potential predators (ants), jumping frequencies were higher than in the control (no ant) arenas, though jumping frequencies decreased after direct contact with the predators. Body mass of B. anurus cocoons induced to jumpsignificantly decreased over time than cocoons that did not jump, suggesting a cost to jumping. We discuss the benefits and costs of jumping behaviour and potential evolutionary advantages of this peculiar trait, which is present in a limited number of species.

DOI： 10.1007/s00114-015-1324-1

Language：English   Publishing type：Research paper (scientific journal)  

The process of loss or gain of parasites during invasion of new lands is not well understood. The alfalfa weevil Hypera postica is an invasive pest of various leguminous crops and consists of three major mitochondrial haplotypes, ‘Western’, ‘Egyptian’ and ‘Eastern’. The Western strain is infected with the endosymbiotic proteobacteria Wolbachia, that cause unidirectional complete reproductive incompatibility, in its native (Europe) and an introduced (the United States) ranges. However, our preliminary screening of a few introduced populations in Northern Kyushu, southwestern Japan, failed to detect Wolbachia from the Western strain. A larger-scale and historical assessment of Wolbachia infection may allow to estimate when and how the bacteria were lost, and current geographical distribution of infection among host haplotypes. In this study, we aim to assess the Wolbachia-infection status of H. postica populations throughout Northern Kyushu, where H. postica invasion to Japan was first found. A total of 228 individuals from seven regions in Northern Kyushu collected in different time periods from 1982 to 2015 and 14 individuals from Europe were subjected to PCR diagnostics for Wolbachia. Wolbachia from the Western strain was not detected, irrespective of the time periods and geographic areas in Northern Kyushu. We found ‘Egyptian’-strain H. postica collected most recently from an island off Kyushu harboured a supergroup-B Wolbachia variant. This variant was genetically different from the European Wolbachia variant infecting Western-strain H. postica. The infection was new to the Egyptian haplotype and was estimated to have taken place independently of the loss in the Western strain.

Repository Public URL： https://hdl.handle.net/2324/7179458

Language：English   Publishing type：Research paper (scientific journal)  

Language：English   Publishing type：Research paper (scientific journal)  

The ultimate cause of genome size (GS) evolution in eukaryotes remains a major and unresolved puzzle in evolutionary biology. Large-scale comparative studies have failed to find consistent correlations between GS and organismal properties, resulting in the ‘C-value paradox’. Current hypotheses for the evolution of GS are based either on the balance between mutational events and drift or on natural selection acting upon standing genetic variation in GS. It is, however, currently very difficult to evaluate the role of selection because within-species studies that relate variation in lifehistory traits to variation in GS are very rare. Here, we report phylogenetic comparative analyses of GS evolution in seed beetles at two distinct taxonomic scales, which combines replicated estimation of GS with experimental assays of life-history traits and reproductive fitness. GS showed rapid and bidirectional evolution across species, but did not show correlated evolution with any of several indices of the relative importance of genetic drift. Within a single species, GS varied by 4–5% across populations and showed positive correlated evolution with independent estimates of male and female reproductive fitness. Collectively, the phylogenetic pattern of GS diversification across and within species in conjunction with the pattern of correlated evolution between GS and fitness provide novel support for the tenet that natural selection plays a key role in shaping GS evolution.

Language：English   Publishing type：Research paper (scientific journal)  

Language：English   Publishing type：Research paper (scientific journal)  

Language：English   Publishing type：Research paper (scientific journal)  

Language：English   Publishing type：Research paper (scientific journal)  

Genetic differentiation can be promoted allopatrically by geographic isolation of populations due to limited dispersal ability and diversification over time or sympatrically through, for example, host-race formation. In crop pests, the trading of crops across the world can lead to intermixing of genetically distinct pest populations. However, our understanding of the importance of allopatric and sympatric genetic differentiation in the face of anthropogenic genetic intermixing is limited. Here, we examined global sequence variation in two mitochondrial and one nuclear genes in the seed beetle Callosobruchus maculatus that uses different legumes as hosts. We analyzed 180 samples from 42 populations of this stored bean pest from tropical and subtropical continents and archipelagos: Africa, the Middle East, South and Southeast Asia, Oceania and South America. For the mitochondrial genes, there was weak but significant genetic differentiation across continents/archipelagos. Further, we found pronounced differentiation among subregions within continents/archipelagos both globally and within Africa but not within Asia. We suggest that multiple introductions into Asia and subsequent intermixing within Asia have generated this pattern. The isolation by distance hypothesis was supported globally (with or without continents controlled) but not when host species was restricted to cowpeas Vigna unguiculata, the ancestral host of C. maculatus. We also document significant among-host differentiation both globally and within Asia, but not within Africa. We failed to reject a scenario of a constant population size in the recent past combined with selective neutrality for the mitochondrial genes. We conclude that mitochondrial DNA differentiation is primarily due to geographic isolation within Africa and to multiple invasions by different alleles, followed by host shifts, within Asia. The weak inter-continental differentiation is most likely due to frequent inter-continental gene flow mediated by human crop trade.

DOI： 10.1371/journal.pone.0106268

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 0.1111/oik.00956

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Language：English   Publishing type：Research paper (scientific journal)  

近年、ミトコンドリア（mt）DNAが、エネルギー生産する酵素複合体を一緒に作る核遺伝子と共進化することが解明されている。これはmt遺伝子と核遺伝子の間のゲノム間エピスタシスが生物個体全体の代謝表現型に影響することを示唆する。そこでヨツモンマメゾウムシの複数のmt系統と核系統間で組み合わせ交配し、代謝率を2つの異なる温度下で測定した。代謝率はmtと核の系統間交互作用と温度に影響された。塩基配列データは、mtの遺伝的変異がこの交互作用の結果を決めることを示唆した。この実験は、２つのゲノムの遺伝的相互作用と遺伝子型x遺伝子型x環境間交互作用を明らかにした。これらの結果は1. 生活史進化一般、特に温度適応の複雑性に洞察を与え、2. 非中立的なmtDNA多型の維持機構を示唆する。
The extent to which mitochondrial DNA (mtDNA) variation is involved in adaptive evolutionary change is currently being reevaluated. In particular, emerging evidence suggests that mtDNA genes coevolve with the nuclear genes with which they interact to form the energy producing enzyme complexes in the mitochondria. This suggests that intergenomic epistasis between mitochondrial and nuclear genes may affect whole-organism metabolic phenotypes. Here, we use crossed combinations of mitochondrial and nuclear lineages of the seed beetle Callosobruchus maculatus and assay metabolic rate under two different temperature regimes. Metabolic rate was affected by an interaction between the mitochondrial and nuclear lineages and the temperature regime. Sequence data suggests that mitochondrial genetic variation has a role in determining the outcome of this interaction. Our genetic dissection of metabolic rate reveals a high level of complexity, encompassing genetic interactions over two genomes, and genotype x genotype x environment interactions. The evolutionary implications of these results are twofold. First, because metabolic rate is at the root of life histories, our results provide insights into the complexity of life-history evolution in general, and thermal adaptation in particular. Second, our results suggest a mechanism that could contribute to the maintenance of nonneutral mtDNA polymorphism.

Language：English   Publishing type：Research paper (scientific journal)  

雌雄はゲノムの大半を共有しながらも選択は異なる。性的対立する遺伝子座は理論上、集団内の性的負荷を引き起こす。しかし、遺伝子座内性的対立(ISC)が移行的な進化的状態（性的二型（SD）の進化により対立は急速に解消）なのか、慢性的な適応障害なのか、は現在明らかでない。同じ条件下では、ISCは雌雄において表現される形質の集団適応度とSDの相関進化として現れるはずだ。そこで同じ飼育環境に適応したマメゾウムシの異なる実験室集団間で適応度とSDを比較しISCの効果を試験した。重要な生活史形質である幼生発育期間のSDは適応度と正の関係があり、これは集団適応度と発育期間の相関進化（雌で正、雄で負）によった。雌雄間の遺伝的結合を緩めることで雌雄がそれぞれの異なる適応度ピークへと近づいたと言える。
Although males and females share much of the same genome, selection is often distinct in the two sexes. Sexually antagonistic loci will in theory cause a gender load in populations, because sex-specific selection on a given trait in one sex will compromise the adaptive evolution of the same trait in the other sex. However, it is currently not clear whether such intralocus sexual conflict (ISC) represents a transient evolutionary state, where conflict is rapidly resolved by the evolution of sexual dimorphism (SD), or whether it is a more chronic impediment to adaptation. All else being equal, ISC should manifest itself as correlated evolution between population fitness and SD in traits expressed in both sexes. However, comparative tests of this prediction are problematic and have been unfeasible. Here, we assess the effects of ISC by comparing fitness and SD across distinct laboratory populations of seed beetles that should be well adapted to a shared environment. We show that SD in juvenile development time, a key life-history trait with a history of sexually antagonistic selection in this model system, is positively related to fitness. This effect is due to a correlated evolution between population fitness and development time that is positive in females but negative in males. Loosening the genetic bind between the sexes has evidently allowed the sexes to approach their distinct adaptive peaks.

Language：English   Publishing type：Research paper (scientific journal)  

母親の環境条件（前世代の環境preE）に依存して、母親から子への投資量（環境E）が変化するとき、適応度を上げるために子の遺伝子（G）の発現も変化すると予測される。アズキゾウムシにおいて、EとGの遺伝相関の影響を取り除くために近交系の雌を用いて、preEとEに対するGの反応を調べた。高密度（preE）飼育した母親との交配では低密度適応した野外系統の父親の子（G）が、低密度（preE）飼育した母親とでは高密度適応した室内系統の父親の子（G）が、卵サイズ（E）の増大とともに発育期間が急激に短くなり（適応度が上がり）予測を支持した。このような密度依存的な母性効果（preE×E×G交互作用）は、新しい環境における急速な適応進化を可能にする。
1. Transgenerational effects, genetic or non-genetic, affect population dynamics and the evolution of life-history traits. Besides genetic components, the size of gametes (eggs and seeds), simultaneously a parental and progeny character, can mediate environmental condition experienced by a parent. In both animals and arthropods, mothers are known to reduce their egg mass depending on their malcondition.
2. Progeny may also modify their life history traits to increase their own fitness when constrained by maternal investment, which may eventually nullify transgenerational effects on population dynamics and evolution. Such fitness modification by the progeny under new environmental conditions requires phenotypic plasticity interacting with egg mass. We hypothesize that different selective environments should produce inter-population genetic diversification of the response to maternal investment on each egg, which would be detected as a paternal genotype x environment x previous (i.e. maternal) environment (G x E x preE) interaction in progeny fitness.
3. To evaluate the contribution of maternal non-genetic resource and the genetic component separately, we used an inbred-isofemale-line approach to eliminate the influence of the genetic correlation between egg mass and other life history traits, in the adzuki bean beetle, Callosobruchus chinensis. The females were reared at either high or low densities to generate variability in egg resources. To test the additive or interactive effect of genotype, non-genetic egg resources, and maternal environment on the life history traits of the progeny, they were crossed with males from laboratory and wild strains that had been subjected to different levels of population density.
4. The G x E x preE interaction effect was detected on the correlation structure between egg mass and development time: In the offspring of mothers reared at low density, the negative correlation between egg mass and development time was higher with lab strain fathers, whereas in the offspring of mothers reared at high density, the negative correlation was higher with the wild strain fathers.
5. Our results indicate a genetic difference in the response of development time but not of adult mass to environmental variation in egg mass. Such density-dependent enhancement of maternal effects may destabilize population dynamics and accelerate evolution.

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DOI： 10.1016/S0065-2504(04)37002-9

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DOI： 10.1016/j.jspr.2004.09.003

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DOI： 10.1079/BER2003277

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DOI： 10.1007/PL00011985

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Language：English   Publisher：Gene  

Argonaute (Ago) proteins act as key elements in RNA interference (RNAi) pathway, orchestrating the intricate machinery of gene regulation within eukaryotic cells. Within the RNAi pathway, small RNA molecules, including microRNA (miRNA), small interfering RNA (siRNA), and PIWI-interacting RNA (piRNA), collaborate with Ago family member proteins such as Ago1, Ago2, and Ago3 to form the RNA-induced silencing complex (RISC). This RISC complex, in turn, either cleaves the target mRNA or inhibits the process of protein translation. The precise contributions of Ago proteins have been well-established in numerous animals and plants, although they still remain unclear in some insect species. This review aims to shed light on the specific roles played by Ago proteins within the RNAi mechanism in a destructive lepidopteran pest, the diamondback moth (Plutella xylostella). Furthermore, we explore the potential of double-stranded RNA (dsRNA)-mediated RNAi as a robust genetic tool in pest management strategies. Through an in-depth examination of Ago proteins and dsRNA-mediated RNAi, this review seeks to contribute to our understanding of innovative approaches for controlling this pest and potentially other insect species of agricultural significance.

DOI： 10.1016/j.gene.2024.148195

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Repository Public URL： https://hdl.handle.net/2324/7179456

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Repository Public URL： https://hdl.handle.net/2324/7179451

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DOI： https://doi.org/10.1186/s41938-020-00332-y

Repository Public URL： https://hdl.handle.net/2324/7173530

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DOI： 10.1007/s42452-019-0960-z

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DOI： 10.1038/sj.hdy.6800720

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DOI： 10.2108/0289-0003(2004)21[105:ANSMSC]2.0.CO;2

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DOI： 10.1079/BER2002218

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DOI： 10.1007/s101440200028

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DOI： 10.1023/A:1006550817371

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DOI： 10.1007/BF02763461

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Language：Japanese   Book type：General book, introductory book for general audience

Event date： 2024.3

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Venue：横浜   Country：Japan  

Event date： 2024.3

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Venue：横浜   Country：Japan  

Event date： 2023.10

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Venue：札幌   Country：Japan  

Event date： 2023.3

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Venue：大阪   Country：Japan  

Event date： 2023.3

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Venue：大阪   Country：Japan  

Event date： 2023.3

Language：English   Presentation type：Public lecture, seminar, tutorial, course, or other speech  

Venue：online   Country：Japan  

Event date： 2022.3

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：オンライン   Country：Japan  

Event date： 2022.3

Language：English   Presentation type：Oral presentation (general)  

Venue：オンライン   Country：Japan  

Event date： 2021.3

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：オンライン   Country：Japan  

Event date： 2021.3

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：オンライン   Country：Japan  

Event date： 2021.3

Language：English   Presentation type：Oral presentation (general)  

Venue：オンライン   Country：Japan  

Event date： 2018.3

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：鹿児島大学   Country：Japan  

Event date： 2017.3

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：早稲田大学、東京   Country：Japan  

Event date： 2016.9

Language：English   Presentation type：Oral presentation (general)  

Venue：Orlando, Florida   Country：United States  

Event date： 2016.9

Language：English   Presentation type：Oral presentation (general)  

Venue：Orlando, Florida   Country：United States  

Event date： 2016.9

Language：English   Presentation type：Oral presentation (general)  

Venue：Orlando, Florida   Country：United States  

Event date： 2016.3

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：大阪   Country：Japan  

Event date： 2016.3

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：大阪   Country：Japan  

Event date： 2016.3

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：大阪   Country：Japan  

Event date： 2016.3

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：大阪   Country：Japan  

Event date： 2015.10

Language：English   Presentation type：Oral presentation (general)  

Venue：Hikone   Country：Japan  

Event date： 2015.9

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：福岡   Country：Japan  

Event date： 2015.9

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：福岡   Country：Japan  

Event date： 2015.9

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：福岡   Country：Japan  

Event date： 2015.9

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：福岡   Country：Japan  

Event date： 2015.9

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：福岡   Country：Japan  

Event date： 2015.9

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：福岡   Country：Japan  

Event date： 2015.6

Language：English   Presentation type：Oral presentation (general)  

Venue：Anchorage, Alaska   Country：United States  

Event date： 2015.3

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：山形   Country：Japan  

Event date： 2015.3

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：山形   Country：Japan  

Event date： 2015.3

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：山形   Country：Japan  

Event date： 2015.3

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：山形   Country：Japan  

Event date： 2015.3

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：山形   Country：Japan  

Event date： 2015.3

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：山形   Country：Japan  

Event date： 2015.3

Language：Japanese   Presentation type：Oral presentation (general)  

Country：Japan  

Event date： 2015.3

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：鹿児島   Country：Japan  

Event date： 2015.2

Language：English   Presentation type：Oral presentation (general)  

Venue：Fukuoka   Country：Japan  

Event date： 2014.12

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：宮崎大学   Country：Japan  

Event date： 2014.11

Language：Japanese  

Venue：長崎   Country：Japan  

Event date： 2014.8

Language：English   Presentation type：Oral presentation (invited, special)  

Venue：Wageningen   Country：Netherlands  

Event date： 2014.6

Language：Japanese   Presentation type：Public lecture, seminar, tutorial, course, or other speech  

Venue：福岡   Country：Japan  

Event date： 2014.3

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：高知   Country：Japan  

Event date： 2014.3

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：高知   Country：Japan  

Event date： 2014.3

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：高知   Country：Japan  

Event date： 2014.3

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：高知   Country：Japan  

Event date： 2014.3

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：広島   Country：Japan  

Event date： 2013.10

Language：English   Presentation type：Oral presentation (general)  

Venue：Osaka   Country：Japan  

Event date： 2013.3

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：日本大学（藤沢市）   Country：Japan  

Event date： 2013.3

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：日本大学（藤沢市）   Country：Japan  

Event date： 2012.10

Language：English   Presentation type：Oral presentation (general)  

Venue：Narashino   Country：Japan  

Event date： 2011.6

Language：English   Presentation type：Oral presentation (invited, special)  

Venue：Antibes   Country：France  

Three-species systems allow an understanding of irregular, complex population dynamics of multiple species assemblages, with emerging effects of indirect interactions. We focused on a laboratory system consisting of a bruchine species Callosobruchus chinensis as a host and two parasitoids, a chalcid Anisopteromalus calandrae and a braconid Heterospilus prosopidis, with each species introduced sequentially.
With a parameterized semi-mechanistic model, we found the introduction of a second parasitoid (H. prosopidis) destabilized the dynamics of host-parasitoid (A. calandrae) interaction towards chaos. Comparison of parameters before and after the introduction indicated that the parameter that was altered by the introduction was solely the mutual interference in A. calandrae. Finally, to confirm the parameter change detected at the population level, we show our behavioral observation through CCD video camera of the interference between A. calandrae females with a H. prosopidis female when parasitizing their common host.

Event date： 2009.8

Presentation type：Oral presentation (invited, special)  

Venue：Awaji   Country：Japan  

Presentation type：Oral presentation (general)  

Venue：福岡   Country：Japan  

Venue：つくば   Country：Japan  

Presentation type：Oral presentation (general)  

Venue：Fukuoka   Country：Japan  

Presentation type：Oral presentation (invited, special)  

Venue：Tokyo   Country：Japan  

Presentation type：Oral presentation (general)  

Venue：Sapporo   Country：Japan  

Event date： 2022.8

Language：English   Presentation type：Oral presentation (general)  

Venue：広島大学   Country：Japan  

Event date： 2018.3

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：鹿児島大学   Country：Japan  

Event date： 2016.11

Language：English  

Venue：Chungnam   Country：Korea, Republic of  

Event date： 2016.11

Language：English  

Venue：Chungnam   Country：Korea, Republic of  

Event date： 2015.10

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：Hikone   Country：Japan  

Event date： 2013.3

Presentation type：Oral presentation (general)  

Venue：日本大学（藤沢市）   Country：Japan  

Event date： 2012.7

Presentation type：Oral presentation (general)  

Venue：Ottawa   Country：Canada  

Event date： 2012.3

Venue：Nara   Country：Japan  

Event date： 2012.3

Presentation type：Oral presentation (general)  

Venue：Nara   Country：Japan  

Event date： 2012.3

Presentation type：Oral presentation (general)  

Venue：Otsu   Country：Japan  

Event date： 2011.10

Presentation type：Oral presentation (general)  

Venue：岡山   Country：Japan  

Event date： 2011.8

Presentation type：Oral presentation (general)  

Country：Japan  

Event date： 2011.3

Presentation type：Oral presentation (general)  

Venue：福岡   Country：Japan  

Event date： 2011.3

Presentation type：Oral presentation (general)  

Venue：福岡   Country：Japan  

Event date： 2011.3

Presentation type：Oral presentation (general)  

Venue：福岡   Country：Japan  

Event date： 2011.3

Presentation type：Oral presentation (general)  

Venue：札幌   Country：Japan  

Event date： 2010.12

Venue：San Diego   Country：United States  

Event date： 2010.12

Presentation type：Oral presentation (general)  

Venue：つくば   Country：Japan  

Event date： 2010.7

Presentation type：Oral presentation (general)  

Venue：Jupiter Beach, FL   Country：United States  

Event date： 2010.3

Presentation type：Oral presentation (general)  

Venue：東京   Country：Japan  

Event date： 2009.11

Presentation type：Oral presentation (general)  

Venue：Tsukuba   Country：Japan  

Event date： 2009.3

Presentation type：Oral presentation (general)  

Venue：札幌   Country：Japan  

Event date： 2009.3

Presentation type：Oral presentation (general)  

Venue：札幌   Country：Japan  

Spatial distribution and functional response of aphid parasitoid, Aphelinus asychis (Hymenoptera: Aphelinidae), attacking green peach aphid, Myzus persicae (Homoptera: Aphididae), in the laboratory

Event date： 2009.3

Venue：札幌   Country：Japan  

Event date： 2009.3

Presentation type：Oral presentation (general)  

Venue：盛岡   Country：Japan  

Event date： 2009.3

Presentation type：Oral presentation (general)  

Venue：盛岡   Country：Japan  

Event date： 2008.10

Presentation type：Oral presentation (general)  

Venue：東京   Country：Japan  

Event date： 2008.10

Presentation type：Symposium, workshop panel (public)  

Venue：東京   Country：Japan  

Event date： 2008.10

Presentation type：Symposium, workshop panel (public)  

Venue：東京   Country：Japan  

Presentation type：Oral presentation (general)  

Venue：福岡   Country：Japan  

Presentation type：Oral presentation (general)  

Venue：Kyoto   Country：Japan  

Presentation type：Oral presentation (general)  

Venue：Manchester   Country：United Kingdom  

Presentation type：Oral presentation (general)  

Venue：Firenze   Country：Italy  

Presentation type：Oral presentation (general)  

Country：Norway  

Presentation type：Oral presentation (general)  

Venue：沖縄   Country：Japan  

Presentation type：Oral presentation (general)  

Venue：Taichung   Country：Taiwan, Province of China  

Presentation type：Oral presentation (general)  

Venue：名古屋   Country：Japan  

Presentation type：Oral presentation (invited, special)  

Venue：熊本   Country：Japan  

Presentation type：Oral presentation (general)  

Venue：Florence   Country：Italy  

Presentation type：Oral presentation (general)  

Venue：Fukuoka   Country：Japan  

Presentation type：Oral presentation (general)  

Country：Japan  

Presentation type：Oral presentation (general)  

Venue：松本   Country：Japan  

Presentation type：Oral presentation (general)  

Venue：Pacific Grove   Country：United States  

Presentation type：Oral presentation (general)  

Country：Japan  

Presentation type：Oral presentation (general)  

Country：Japan  

Presentation type：Oral presentation (general)  

Venue：松江   Country：Japan  

Presentation type：Oral presentation (general)  

Venue：松江   Country：Japan  

Presentation type：Oral presentation (general)  

Venue：熊本   Country：Japan  

Presentation type：Oral presentation (general)  

Venue：熊本   Country：Japan  

Presentation type：Oral presentation (general)  

Country：Japan  

Presentation type：Oral presentation (general)  

Venue：仙台   Country：Japan  

Presentation type：Oral presentation (general)  

Venue：山形   Country：Japan  

Presentation type：Oral presentation (general)  

Venue：東京   Country：Japan  

Presentation type：Oral presentation (general)  

Venue：東京   Country：Japan  

Presentation type：Oral presentation (general)  

Venue：東京   Country：Japan  

Presentation type：Oral presentation (general)  

Venue：仙台   Country：Japan  

Presentation type：Oral presentation (general)  

Venue：Seoul   Country：Korea, Republic of  

Presentation type：Oral presentation (general)  

Venue：富山   Country：Japan  

Presentation type：Oral presentation (general)  

Venue：Fukuoka   Country：Japan  

Presentation type：Oral presentation (general)  

Venue：盛岡   Country：Japan  

Presentation type：Oral presentation (general)  

Venue：つくば   Country：Japan  

Presentation type：Oral presentation (general)  

Venue：京都   Country：Japan  

Presentation type：Oral presentation (general)  

Venue：京都   Country：Japan  

Presentation type：Oral presentation (general)  

Venue：京都   Country：Japan  

Presentation type：Oral presentation (general)  

Venue：京都   Country：Japan  

Venue：釧路   Country：Japan  

Presentation type：Symposium, workshop panel (public)  

Venue：大阪   Country：Japan  

Presentation type：Oral presentation (general)  

Venue：大阪   Country：Japan  

Presentation type：Oral presentation (general)  

Venue：東京   Country：Japan  

Presentation type：Oral presentation (invited, special)  

Venue：東京   Country：Japan  

Venue：仙台   Country：Japan  

Presentation type：Oral presentation (invited, special)  

Venue：Kaga   Country：Japan  

Presentation type：Oral presentation (invited, special)  

Venue：函館   Country：Japan  

Venue：新潟   Country：Japan  

Venue：つくば   Country：Japan  

Presentation type：Oral presentation (general)  

Venue：福岡   Country：Japan  

Language：Japanese  

Language：Japanese   Publishing type：Article, review, commentary, editorial, etc. (scientific journal)  

Time-series analysis

Language：Japanese   Publishing type：Article, review, commentary, editorial, etc. (scientific journal)  

Detecting chaos from time-series of population dynamics

Language：Japanese   Publishing type：Article, review, commentary, editorial, etc. (scientific journal)  

Mechanism for coexistence and persistence of laboratory community of bean-bruchid-parasitoid

Type：Peer review 

Number of peer-reviewed articles in foreign language journals：13

Type：Competition, symposium, etc. 

Number of participants：2,300

Type：Peer review 

Number of peer-reviewed articles in foreign language journals：13

Type：Scientific advice/Review 

Type：Peer review 

Number of peer-reviewed articles in foreign language journals：11

Type：Academic society, research group, etc. 

Type：Peer review 

Number of peer-reviewed articles in foreign language journals：4

Type：Peer review 

Number of peer-reviewed articles in foreign language journals：10

Number of peer-reviewed articles in Japanese journals：1

Type：Competition, symposium, etc. 

Number of participants：240

Type：Competition, symposium, etc. 

Number of participants：120

Type：Scientific advice/Review 

Type：Peer review 

Number of peer-reviewed articles in foreign language journals：22

Type：Competition, symposium, etc. 

Number of participants：350

Type：Scientific advice/Review 

Type：Scientific advice/Review 

Type：Competition, symposium, etc. 

Number of participants：2,500

Type：Scientific advice/Review 

Type：Competition, symposium, etc. 

Number of participants：2,160

Type：Competition, symposium, etc. 

Type：Competition, symposium, etc. 

Number of participants：6,300

Type：Competition, symposium, etc. 

Number of participants：2,000

Type：Academic society, research group, etc. 

Type：Scientific advice/Review 

Type：Competition, symposium, etc. 

Number of participants：200

Type：Competition, symposium, etc.