Updated on 2024/09/09

Information

 

写真a

 
LEE MAN
 
Organization
Faculty of Agriculture Department of Bioresource Sciences Professor
School of Agriculture Department of Bioresource and Bioenvironment(Joint Appointment)
Graduate School of Bioresource and Bioenvironmental Sciences Department of Bioresource Sciences(Joint Appointment)
Title
Professor
Contact information
メールアドレス
Tel
0928024788
Profile
1. 物質生産宿主としてのカイコの分子育種  [九州大学保存系統に着目し、バキュロウイルス-蚕発現系におけるタンパク質発現宿主としてのカイコの有用性を検討すると共に、その付加価値を更に高めるため、カイコの分子育種を行った。まずは、九州大学の保持するカイコバイオリソースを元にし、普及しているAcNPV系をそのまま利用できる蚕系統を開発した。約450系統を用いてスクリーニングを行い、10系統のAcNPV高感受性系統を見出した。次いで、蚕を宿主とするBmNPVを用いて、組換えタンパク質高生産性を示す系統を見出した。これらの研究成果から、AcNPVとBmNPVの両方が利用可能な蚕系統と難生産性タンパク質の安定的な発現に適した系統が確立できた。さらに、高感受性に寄与する分子機構 (原因遺伝子)も解明した。しかし、九州大学保存の蚕系統を用いても発現が困難なタンパク質も多数存在する。この場合は、カイコバイオリソースに遺伝子組換え技術を利用し、量的・質的に改善した組換えタンパク質を発現させ、そのタンパク質の機能解析を行っている。] 2. 昆虫細胞で生産するタンパク質の高付加価値化と新規機能デザイン [これまでの成果をもとに、産業用有用タンパク質の生産を開始し、サイトカイン、成長因子、分化誘導因子などについて、九州大学保存の蚕系統の高い生産能力を実証してきた。さらに、産業上、高い需要がありながらも発現が難しいタンパク質の発現についても改良を重ねてきた。例えば、ヘムを補助因子として有するタンパク質も、ヘム前駆体である5-ALAを予め投与しておくことにより、大量に生産できることを明らかにした。また、マラリア抗原にDNA結合ドメインを融合し、DNA上で高分子構造を形成することにより、より高いワクチン効果を持つタンパク質の生産に成功している。さらに、部位特異的タンパク質修飾技術を取入れ、蚕で生産したウイルス様粒子(VLP)の表面に他のタンパク質を提示する技術も確立した。一方、自然界にない新規機能や性質を持つタンパク質を多数デザイン・大量生産し、その構造と機能を検証し、その有用性も示して来た。また、タンパク質の糖鎖修飾の制御や機能ドメインの融合、生体高分子複合体の生産技術などを開発した。] 3. ベクターとしての昆虫感染性ウイルスの利用 [まずは、鱗翅目昆虫を対象とした外来遺伝子の過剰発現を高効率かつ容易に行うことができる汎用ベクターシステムの構築を行った。構築した汎用ベクターをウイルスベクターに転移し、培養細胞と個体組織レベルでの外来遺伝子の導入効率を明らかにした。さらに、BmN4-SID1,Bme21-SID1細胞、ヨトウガSf9-SID1細胞を樹立し、培地にウイルスDNAを添加するだけ細胞が能動的にDNAを取込むSoaking法も改良し、ベクターとしての昆虫感染性ウイルスの利用が簡便に活用可能であることを明らかにした。]

Degree

  • Ph.D

Research Interests・Research Keywords

  • Research theme:Analysis and modification of protein glycosylation in an insect cell expression system

    Keyword:Glycosylation, Baculovirus expression system, N-type sugar chain, O-type sugar chain

    Research period: 2015.4

  • Research theme:Study on high efficient recombinant protein production using baculovirus

    Keyword:Silkworm, Baculovirus, recombinant protein

    Research period: 2004.10

  • Research theme:Construction of vector system for lepidopteran gene functional analysis

    Keyword:Silkworm, Constitutive gene expression system, Constitutive promoter

    Research period: 2000.10

Awards

  • 貞明皇后記念蚕糸科学賞

    2019.11   大日本蚕糸会   令和元年貞明皇后記念蚕糸科学賞

  • 蚕糸学進歩賞(技術賞)

    2009.3   社団法人日本蚕糸学会(1930年創設)   平成21年度蚕糸学進歩賞[技術賞]

  • 蚕糸学進歩賞(技術賞)

    2008.3   社団法人日本蚕糸学会(1930年創設)   平成19年度第43回蚕糸学進歩賞(技術賞)

Papers

  • Comprehensive Transcriptome Analysis in the Testis of the Silkworm, Bombyx mori Reviewed

    #Kohei Kakino, @Hiroaki Mon, #Takeru Ebihara, @Masato Hino, @Akitsu Masuda, @Jae Man Lee, @Takahiro Kusakabe

    Insects   14 ( 8 )   684 - 684   2023.8

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: https://doi.org/10.3390/insects14080684

  • The biological role of core 1β1-3galactosyltransferase (T-synthase) in mucin-type O-glycosylation in Silkworm, Bombyx mori Reviewed

    156   103936 - 103936   2023.5

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.ibmb.2023.103936

  • High yield production of norovirus GII. 4 virus-like particles using silkworm pupae and evaluation of their protective immunogenicity Reviewed

    @Masuda A, @Lee JM, @Miyata T, @Sato S, @Masuda A, @Taniguchi M, @Fujita R, @Ushijima H, #Morimoto K, #Ebihara T, @Hino M, #Kakino K, @Mon H, @Kusakabe T

    Vaccine   41 ( 3 )   766 - 777   2023.1

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.vaccine.2022.12.015

  • TRPC3-Nox2 Protein Complex Formation Increases the Risk of SARS-CoV-2 Spike Protein-Induced Cardiomyocyte Dysfunction through ACE2 Upregulation. Reviewed

    @Kato Y, @Nishiyama K, @Lee JM, @Ibuki Y, @Imai Y, @Noda T, @Kamiya N, @Kusakabe T, @Kanda Y, @Nishida M.

    International Journal of Molecular Sciences   24 ( 1 )   2022.12

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.3390/ijms24010102

  • Highly efficient protein expression of Plasmodium vivax surface antigen, Pvs25, by silkworm and its biochemical analysis Reviewed

    @Miyata T, @Minamihata K, @Kurihara K, @Kamizuru Y, @Gotanda M, @Obayashi M, @Kitagawa T, @Sato K, @Kimura M, #Oyama K, @Ikeda Y, @Tamaki Y, @Lee JM, @Sakao K, @Hamanaka D, @Kusakabe T, @Tachibana M, @Ibrahim HR.

    Protein Expression and Purification   2022.6

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  • Optimization of SARS-CoV-2 Spike Protein Expression in the Silkworm and Induction of Efficient Protective Immunity by Inoculation With Alum Adjuvants. Reviewed

    #Masuda A, @LeeJM, @Miyata T, @Mon H, @Sato K, #Oyama K, @Sakurai Y, @Yasuda J, @Takahashi D, @Ueda T, @Kato Y, @Nishida M, @Karasaki N, @Kakino K, #Ebihara T, #Nagasato T, @Hino M, @Nakashima A, @Suzuki K, #Tonooka Y, #Tanaka M, #Moriyama T, @Nakatake H, @Fujita R, @Kusakabe T.

    Frontiers in immunology   12   803647   2022.1

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.3389/fimmu.2021.803647

  • Leptin Is an Important Endocrine Player That Directly Activates Gonadotropic Cells in Teleost Fish, Chub Mackerel Reviewed

    @Ohga H, @Ito K, #Kakino K, @Mon H, @Kusakabe T, @Lee JM, @Matsuyama M.

    Cells   10 ( 12 )   3505   2021.12

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.3390/cells10123505

  • Production of scFv, Fab, and IgG of CR3022 Antibodies Against SARS-CoV-2 Using Silkworm-Baculovirus Expression System Reviewed

    #Ebihara T, #Masuda A, @Takahashi D, @Hino M, @Mon H, #Kakino K, @Fujii T, @Fujita R, @Ueda T, @Lee JM, @Kusakabe T.

    Molecular biotechnology   63 ( 12 )   1223 - 1234   2021.12

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1007/s12033-021-00373-0

  • Stable trimer formation of spike protein from porcine epidemic diarrhea virus improves the efficiency of secretory production in silkworms and induces neutralizing antibodies in mice Reviewed

    #Masuda A, @Lee JM, @Miyata T, #Ebihara T, #Kakino K, @Hino M, @Fujita R, @Mon H, @Kusakabe T.

    Veterinary Research   52 ( 1 )   1 - 14   2021.7

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1186/s13567-021-00971-5

  • Active Human and Murine Tumor Necrosis Factor α Cytokines Produced from Silkworm Baculovirus Expression System Reviewed

    12 ( 6 )   517   2021.6

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    DOI: https://doi.org/10.3390/insects12060517

  • Production of an active Mus musculus IL-3 using updated silkworm-based baculovirus expression vector system Reviewed

    #Ryo Nagai, #Takeru Ebihara, #Kohei Kakino, #Akitsu Masuda, #Jian Xu, @Kosuke Minamihata, @Noriho Kamiya, @Tatphon Kongkrongtong, @Masahiro Kawahara, @Hiroaki Mon, @Tsuguru Fujii, @Takahiro Kusakabe, @Jae Man Lee

    Journal of Asia-Pacific Entomology   2021.4

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: https://doi.org/10.1016/j.aspen.2021.04.011

  • Clomipramine suppresses ACE2-mediated SARS-CoV-2 entry Reviewed

    Yuri Kato, Shigeru Yamada, Kazuhiro Nishiyama, Ayano Satsuka, Suyong Re, Daiki Tomokiyo, Jae Man Lee,Tomohiro Tanaka, Akiyuki Nishimura, Kenzo Yonemitsu, Hiroshi Asakura, Yuko Ibuki, Yumiko Imai, Noriho Kamiya, Kenji Mizuguchi, Takahiro Kusakabe, Yasunari Kanda, Motohiro Nishida

    bioRxiv   2021.3

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    DOI: https://doi.org/10.1101/2021.03.13.435221

  • Quick and Easy Assembly of a One-Step qRT-PCR Kit for COVID-19 Diagnostics Using In-House Enzymes Reviewed

    Masateru Takahashi, Muhammad Tehseen, Rahul Salunke, Etsuko Takahashi, Sara Mfarrej, Mohamed A Sobhy, Fatimah S Alhamlan, Sharif Hala, Gerardo Ramos-Mandujano, Ahmed A Al-Qahtani, Fadwa S Alofi, Afrah Alsomali, Anwar M Hashem, Asim Khogeer, Naif AM Almontashiri, Jae Man Lee, Hiroaki Mon, Kosuke Sakashita, Mo Li, Takahiro Kusakabe, Arnab Pain, Samir M Hamdan

    ACS omega   6 ( 11 )   7374 - 7386   2021.3

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1021/acsomega.0c05635

  • Using Eukaryotic Expression Systems to Generate Human α1, 3-Fucosyltransferases That Effectively Create Selectin-Binding Glycans on Stem Cells Reviewed

    59 ( 39 )   3757 - 3771   2020.9

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1021/acs.biochem.0c00523

  • Efficient production of recombinant SARS-CoV-2 spike protein using the baculovirus-silkworm system Reviewed

    Ryosuke Fujita, Masato Hino, Takeru Ebihara, Takumi Nagasato, Akitsu Masuda, Jae Man Lee, Tsuguru Fujii, Hiroaki Mon, Kohei Kakino, Ryo Nagai, Miyu Tanaka, Yoshino Tonooka, Takato Moriyama, Takahiro Kusakabe

    Biochemical and Biophysical Research Communications   529 ( 2 )   257 - 262   2020.8

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    DOI: 10.1016/j.bbrc.2020.06.020

  • A defect in purine nucleotide metabolism in the silkworm, Bombyx mori, causes a translucent larval integument and male infertility Reviewed

    Tsuguru Fujii, Kohei Kakino, Miyu Tanaka, Jae Man Lee, Takahiro Kusakabe, Yutaka Banno

    Insect Biochemistry and Molecular Biology   2020.8

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.ibmb.2020.103458

  • Efficient production of recombinant T7 endonuclease I using silkworm-baculovirus expression vector system Reviewed

    Kohei Kakino, Akitsu Masuda, Masato Hino, Takeru Ebihara, Jian Xu, Hiroaki Mon, Ryosuke Fujita, Tsuguru Fujii, Takahiro Kusakabe, Jae Man Lee

    Journal of Asia-Pacific Entomology   23 ( 3 )   694 - 700   2020.8

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.aspen.2020.05.001

  • Optimal silkworm larva host for high-level production of Mus musculus IL-4 using a baculovirus expression vector system Reviewed

    Masahiko Kobayashi, Jian Xu, Kohei Kakino, Akitsu Masuda, Masato Hino, Naoki Fujimoto, Kosuke Minamihata, Noriho Kamiya, Hiroaki Mon, Hiroshi Iida, Masateru Takahashi, Takahiro Kusakabe, Jae Man Lee

    Journal of Asia-Pacific Entomology   23 ( 1 )   268 - 273   2020.4

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.aspen.2019.12.014

  • Expression and Purification of Vaccinia Virus DNA Topoisomerase IB Produced in the Silkworm–Baculovirus Expression System Reviewed

    61 ( 8 )   622 - 630   2019.8

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    Type IB DNA topoisomerases are enzymes to change the topological state of DNA molecules and are essential in studying replication, transcription, and recombination of nucleic acids in vitro. DNA topoisomerase IB from Vaccinia virus (vTopIB) is a 32 kDa, type I eukaryotic topoisomerase, which relaxed positively and negatively supercoiled DNAs without Mg2+ and ATP. Although vTopIB has been effectively produced in E. coli expression system, no studies remain available to explore an alternative platform to express recombinant vTopIB (rvTopIB) in a higher eukaryote, where the one can expect post-translational modifications that affect the activity of rvTopIB. Here in this study, rvTopIB with N-terminal tags was constructed and expressed in a silkworm–baculovirus expression vector system (silkworm–BEVS). We developed a simple two consecutive chromatography purification to obtain highly pure rvTopIB. The final yield of rvTopIB obtained from a baculovirus-infected silkworm larva was 83.25 μg. We also evaluated the activity and function of rvTopIB by the DNA relaxation activity assays using a negatively supercoiled pUC19 plasmid DNA as a substrate. With carefully assessing optimized conditions for the reaction buffer, we found that divalent ions, Mg2+, Mn2+, Ca2+, as well as ATP stimulate the DNA relaxation activity by rvTopIB. The functional and active form of rvTopIB, together with the yields of the protein we obtained, suggests that silkworm–BEVS would be a potential alternative platform to produce eukaryotic topoisomerases on an industrial scale.

    DOI: 10.1007/s12033-019-00184-4

  • Expression and purification of biologically active human granulocyte-macrophage colony stimulating factor (hGM-CSF) using silkworm-baculovirus expression vector system Reviewed

    Yurie Kinoshita, Jian Xu, Akitsu Masuda, Kosuke Minamihata, Noriho Kamiya, Hiroaki Mon, Ryosuke Fujita, Takahiro Kusakabe, Man Lee

    Protein Expression and Purification   159   69 - 74   2019.7

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    DOI: 10.1016/j.pep.2019.03.010

  • Expression of the thermostable Moloney murine leukemia virus reverse transcriptase by silkworm-baculovirus expression system Reviewed

    Takumi Yano, Man Lee, Jian Xu, Yoshiki Morifuji, Akitsu Masuda, Masato Hino, Daisuke Morokuma, Ryosuke Fujita, Masateru Takahashi, Takahiro Kusakabe, Hiroaki Mon

    Journal of Asia-Pacific Entomology   22 ( 2 )   453 - 457   2019.6

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    DOI: 10.1016/j.aspen.2019.02.008

  • Expression, purification, and characterization of highly active endo-α-N-acetylgalactosaminidases expressed by silkworm-baculovirus expression system Reviewed

    22 ( 2 )   404 - 408   2019.6

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    The O-glycosidase, endo-α-N-acetylgalactosaminidase from Enterococcus faecalis (endoEF) catalyzes the cleavage of core 1 and core 3 type O-linked disaccharides between GalNAc and serine or threonine residues from glycoproteins. The endoEF has broad substrate specificity and thus is extensively utilized for the structural and functional analysis of the O-linked glycans. In this study, we expressed and purified the recombinant endoEF (rEndoEF) by using the silkworm-baculovirus expression vector system (Silkworm-BEVS) and confirmed the deglycosylation activity of rEndoEF targeting reporter glycoproteins, which was equivalent to the commercial O-glycosidase. Thus, our study provides important clues to produce highly active rEndoEF O-glycosidases employing silkworm-BEVS as an alternative.

    DOI: 10.1016/j.aspen.2019.01.009

  • Functional horseradish peroxidase−streptavidin chimeric proteins prepared using a silkworm-baculovirus expression system for diagnostic purposes Reviewed

    297   28 - 31   2019.5

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    Rapid, convenient, sensitive detection methods are of the utmost importance in analytical tools. Enzyme-based signal amplification using horseradish peroxidase (HRP) is commonly implemented in clinical diagnostics kits based on enzyme-linked immunosorbent assay (ELISA), by which the limit of detection is greatly improved. Herein we report the design and preparation of recombinant fusion proteins comprising HRP and streptavidin (Stav), in which HRP was fused to either the N- or C-terminus of Stav ((HRP)
    4
    –Stav or Stav–(HRP)
    4
    , respectively) using a baculovirus-silkworm expression system. Both (HRP)
    4
    –Stav and Stav–(HRP)
    4
    were secreted in the apo form but they were easily converted to the holo form and activated by simple incubation with hemin overnight at 4 °C. The activated (HRP)
    4
    –Stav and Stav–(HRP)
    4
    could be combined with a commercial biotinylated anti-OVA IgG antibody to detect ovalbumin (OVA) as the antigen in ELISA. The enzymatic activity of (HRP)
    4
    –Stav was twofold higher than that of Stav–(HRP)
    4
    , and the sensitivity of (HRP)
    4
    -Stav in ELISA was higher than that of a commercial HRP–Stav chemical conjugate. The successful use of (HRP)
    4
    –Stav chimeric protein as a molecular probe in ELISA shows that the baculovirus-silkworm expression system is promising to produce enzyme–Stav conjugates to substitute for those prepared by chemical methods.

    DOI: 10.1016/j.jbiotec.2019.03.007

  • A functional polypeptide N-acetylgalactosaminyltransferase (PGANT) initiates O-glycosylation in cultured silkworm BmN4 cells Reviewed

    Jian Xu, Akihiro Morio, Daisuke Morokuma, Yudai Nagata, Masato Hino, Akitsu Masuda, Zhiqing Li, Hiroaki Mon, Takahiro Kusakabe, Man Lee

    Applied Microbiology and Biotechnology   102 ( 20 )   8783 - 8797   2018.10

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1007/s00253-018-9309-6

  • Expression and Activation of Horseradish Peroxidase–Protein A/G Fusion Protein in Silkworm Larvae for Diagnostic Purposes Reviewed

    13 ( 6 )   2018.6

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    Recombinant protein production can create artificial proteins with desired functions by introducing genetic modifications to the target proteins. Horseradish peroxidase (HRP) has been used extensively as a reporter enzyme in biotechnological applications; however, recombinant production of HRP has not been very successful, hampering the utilization of HRP with genetic modifications. A fusion protein comprising an antibody binding protein and HRP will be an ideal bio-probe for high-quality HRP-based diagnostic systems. A HRP-protein A/G fusion protein (HRP-pAG) is designed and its production in silkworm (Bombyx mori) is evaluated for the first time. HRP-pAG is expressed in a soluble apo form, and is activated successfully by incubating with hemin. The activated HRP-pAG is used directly for ELISA experiments and retains its activity over 20 days at 4 °C. Moreover, HRP-pAG is modified with biotin by the microbial transglutaminase (MTG) reaction. The biotinylated HRP-pAG is conjugated with streptavidin to form a HRP-pAG multimer and the multimeric HRP-pAG produced higher signals in the ELISA system than monomeric HRP-pAG. The successful production of recombinant HRP in silkworm will contribute to creating novel HRP-based bioconjugates as well as further functionalization of HRP by applying enzymatic post-translational modifications.

    DOI: 10.1002/biot.201700624

  • Production of a biologically active human basic fibroblast growth factor using silkworm-baculovirus expression vector system Reviewed

    Akitsu Masuda, Jian Xu, Kosuke Minamihata, Genki Kagawa, Yusei Hamada, Yoshiki Morifuji, Takumi Yano, Masato Hino, Daisuke Morokuma, Noriko Karasaki, Hiroaki Mon, Noriho Kamiya, Takahiro Kusakabe, Man Lee

    Journal of Asia-Pacific Entomology   21 ( 2 )   716 - 720   2018.6

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    DOI: 10.1016/j.aspen.2018.05.002

  • Expression, Purification, and Characterization of Recombinant Human α 1 -Antitrypsin Produced Using Silkworm–Baculovirus Expression System Reviewed

    2018.1

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    Human α
    1
    -antitrypsin (AAT) is the most abundant serine proteinase inhibitor (serpin) in the human plasma. Commercially available AAT for the medications of deficiency of α
    1
    -antitrypsin is mainly purified from human plasma. There is a high demand for a stable and low-cost supply of recombinant AAT (rAAT). In this study, the baculovirus expression vector system using silkworm larvae as host was employed and a large amount of highly active AAT was recovered from the silkworm serum (~ 15 mg/10 ml) with high purity. Both the enzymatic activity and stability of purified rAAT were comparable with those of commercial product. Our results provide an alternative method for mass production of the active rAAT in pharmaceutical use.

    DOI: 10.1007/s12033-018-0127-y

  • Structure–function analyses of a stereotypic rheumatoid factor unravel the structural basis for germline-encoded antibody autoreactivity Reviewed

    293 ( 18 )   7008 - 7016   2018.1

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    Rheumatoid factors (RFs) are autoantibodies against the fragment-crystallizable (Fc) region of IgG. In individuals with hematological diseases such as cryoglobulinemia and certain B cell lymphoma forms, the RFs derived from specific heavy- and light-chain germline pairs, so-called “stereotypic RFs,” are frequently produced in copious amounts and form immune complexes with IgG in serum. Of note, many structural details of the antigen recognition mechanisms in RFs are unclear. Here we report the crystal structure of the RF YES8c derived from the IGHV1-69/IGKV3-20 germline pair, the most common of the stereotypic RFs, in complex with human IgG1-Fc at 2.8 Å resolution. We observed that YES8c binds to the CH2–CH3 elbow in the canonical antigen-binding manner involving a large antigen–antibody interface. On the basis of this observation, combined with mutational analyses, we propose a recognition mechanism common to IGHV1-69/IGKV3-20 RFs: (1) the interaction of the Leu
    432
    –His
    435
    region of Fc enables the highly variable complementarity-determining region (CDR)-H3 to participate in the binding, (2) the hydrophobic tip in the CDR-H2 typical of IGHV1-69 antibodies recognizes the hydrophobic patch on Fc, and (3) the interaction of the highly conserved RF light chain with Fc is important for RF activity. These features may determine the putative epitope common to the IGHV1-69/IGKV3-20 RFs. We also showed that some mutations in the binding site of RF increase the affinity to Fc, which may aggravate hematological diseases. Our findings unravel the structural basis for germline-encoded antibody autoreactivity.

    DOI: 10.1074/jbc.M117.814475

  • Purification and characterization of immunogenic recombinant virus-like particles of porcine circovirus type 2 expressed in silkworm pupae Reviewed

    Akitsu Masuda, Man Lee, Takeshi Miyata, Tetsuo Sato, Shizuka Hayashi, Masato Hino, Daisuke Morokuma, Noriko Karasaki, Hiroaki Mon, Takahiro Kusakabe

    Journal of General Virology   99 ( 7 )   917 - 926   2018.1

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    DOI: 10.1099/jgv.0.001087

  • Modular surface display for porcine circovirus type 2 virus-like particles mediated by microbial transglutaminase Reviewed

    Akitsu Masuda, Kosuke Minamihata, Masato Hino, Daisuke Morokuma, Noriko Karasaki, Hiroaki Mon, Noriho Kamiya, Takahiro Kusakabe, Man Lee

    Journal of Insect Biotechnology and Sericology   87 ( 2 )   53 - 60   2018.1

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    DOI: 10.11416/jibs.87.2_053

  • Characterization of Recombinant Thermococcus kodakaraensis (KOD) DNA Polymerases Produced Using Silkworm-Baculovirus Expression Vector System Reviewed

    Mami Yamashita, Jian Xu, Daisuke Morokuma, Kazuma Hirata, Masato Hino, Hiroaki Mon, Masateru Takahashi, Samir M. Hamdan, Kosuke Sakashita, Kazuhiro Iiyama, Yutaka Banno, Takahiro Kusakabe, Jae Man Lee

    Molecular Biotechnology   59 ( 6 )   221 - 233   2017.6

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    DOI: 10.1007/s12033-017-0008-9

  • Expression and Characterization of Human β-1, 4-Galactosyltransferase 1 (β4GalT1) Using Silkworm–Baculovirus Expression System Reviewed

    59 ( 4-5 )   151 - 158   2017.5

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    Baculovirus expression vector system (BEVS) is widely known as a mass-production tool to produce functional recombinant glycoproteins except that it may not be always suitable for medical practice due to the differences in the structure of N-linked glycans between insects and mammalian. Currently, various approaches have been reported to alter N-linked glycan structures of glycoproteins derived from insects into terminally sialylated complex-type N-glycans. In the light of those studies, we also proposed in vitro maturation of N-glycan with mass-produced and purified glycosyltransferases by silkworm–BEVS. β-1,4-Galactosyltransferase 1 (β4GalT1) is known as one of type II transmembrane enzymes that transfer galactose in a β-1, 4 linkage to accepter sugars, and a key enzyme for further sialylation of N-glycans. In this study, we developed a large-scale production of recombinant human β4GalT1 (rhβ4GalT1) with N- or C-terminal tags in silkworm–BEVS. We demonstrated that rhβ4GalT1 is N-glycosylated and without mucin-type glycosylation. Interestingly, we found that purified rhβ4GalT1 from silkworm serum presented higher galactosyltransferase activity than that expressed from cultured mammalian cells. We also validated the UDP-galactose transferase activity of produced rhβ4GalT1 proteins by using protein subtracts from silkworm silk gland. Taken together, rhβ4GalT1 from silkworms can become a valuable tool for producing high-quality recombinant glycoproteins with mammalian-like N-glycans.

    DOI: 10.1007/s12033-017-0003-1

  • Proteasome inhibitor MG132 impairs autophagic flux through compromising formation of autophagosomes in Bombyx cells Reviewed International journal

    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS   479 ( 4 )   690 - 696   2016.10

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    DOI: 10.1016/j.bbrc.2016.09.151

  • Co-expression of silkworm allatostatin-C receptor BNGR-A1 with its cognate G protein subunits enhances the GPCR display on the budding baculovirus Reviewed International journal

    Journal of Asia-Pacific Entomology   19 ( 3 )   753 - 760   2016.9

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    DOI: 10.1016/j.aspen.2016.07.007

  • High-level expression and purification of biologically active human IL-2 using silkworm-baculovirus expression vector system. Reviewed International journal

    Journal of Asia-Pacific Entomology   19   313 - 317   2016.6

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    DOI: 10.1016/j.aspen.2016.03.014

  • Molecular analysis and bioactivity of luteinizing hormone from Japanese eel, Anguilla japonica, produced in silkworm pupae Reviewed International journal

    Ji-Hyun Choi, Dae-Jung Kim, Sun Mee Hong, Sun-Jung Jo, Kwan-Sik Min, Young Chang Sohn, JAE MAN LEE, takahiro kusakabe

    BIOTECHNOLOGY AND BIOPROCESS ENGINEERING   21 ( 3 )   381 - 388   2016.6

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    DOI: 10.1007/s12257-016-0042-7

  • Expression and Characterization of Recombinant Serratia liquefaciens Nucleases Produced with Baculovirus-mediated Silkworm Expression System Reviewed International journal

    MOLECULAR BIOTECHNOLOGY   58 ( 6 )   393 - 403   2016.6

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    DOI: 10.1007/s12033-016-9937-y

  • Characterization of the roles of DNA polymerases, clamp, and clamp loaders during S-phase progression and cell cycle regulation in the silkworm, Bombyx mori Reviewed International journal

    Journal of Insect Biotechnology and Sericology   85   21 - 29   2016.6

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  • Comparative proteomic analysis of hemolymph proteins from Autographa californica multiple nucleopolyhedrovirus (AcMNPV)-sensitive or -resistant silkworm strains during infections

    COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS   16   36 - 47   2015.12

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    DOI: 10.1016/j.cbd.2015.07.003

  • Roles of silkworm endoplasmic reticulum chaperones in the secretion of recombinant proteins expressed by baculovirus system Reviewed International journal

    MOLECULAR AND CELLULAR BIOCHEMISTRY   409 ( 1-2 )   255 - 262   2015.11

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    DOI: 10.1007/s11010-015-2529-5

  • Heme precursor injection is effective for Arthromyces ramosus peroxidase fusion protein production by a silkworm expression system Reviewed International journal

    JOURNAL OF BIOSCIENCE AND BIOENGINEERING   120 ( 4 )   384 - 386   2015.10

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    DOI: 10.1016/j.jbiosc.2015.02.013

  • Mass Production of an Active Peptide-N-Glycosidase F Using Silkworm-Baculovirus Expression System Reviewed International journal

    MOLECULAR BIOTECHNOLOGY   57 ( 8 )   735 - 745   2015.8

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    DOI: 10.1007/s12033-015-9866-1

  • Improvement of Endo-β-N-acetylglucosaminidase H production using silkworm–baculovirus protein expression system Reviewed International journal

    Atsushi Masuda, Jian Xu, Takumi Mitsudome, Daisuke Morokuma, 門 宏明, YUTAKA BANNO, takahiro kusakabe, JAE MAN LEE

    18 ( 2 )   175 - 180   2015.6

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  • Human alpha 1-acid glycoprotein as a model protein for glycoanalysis in baculovirus expression vector system Reviewed International journal

    Journal of Asia-Pacific Entomology   18 ( 2 )   303 - 309   2015.6

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  • Differential N-Glycan Modifications of Human Alpha 1-Acid Glycoprotein (α1AGP) Produced in Different Silkworm Strains using the Baculovirus Expression System Reviewed International journal

    Daisuke Morokuma, 門 宏明, Banno Y, takahiro kusakabe, JAE MAN LEE

    84 ( 2 )   49 - 53   2015.5

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  • Characterization and evolutionary analysis of tributyltin‐binding protein and pufferfish saxitoxin and tetrodotoxin‐binding protein genes in toxic and nontoxic pufferfishes Reviewed International journal

    28 ( 5 )   1103 - 1108   2015.5

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  • A novel third chromosomal locus controls susceptibility to Autographa californica multiple nucleopolyhedrovirus in the silkworm, Bombyx mori.

    Applied Microbiology and Biotechnology   98 ( 7 )   3049 - 3058   2014.4

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  • Baculovirus-mediated gene transfer systems in silkworm larvae using constitutive host promoters

    JOURNAL OF ASIA-PACIFIC ENTOMOLOGY   17 ( 1 )   2014.3

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    DOI: 10.1016/j.aspen.2013.10.009

  • RNAi suppression of β-N-acetylglucosaminidase (BmFDL) for complex-type N-linked glycan synthesis in cultured silkworm cells Reviewed International journal

    Yudai Nagata, JAE MAN LEE, 門 宏明, Shigeo Imanishi, Sun Mee Hong,, Shoji Komatsu, Yuji Oshima, takahiro kusakabe

    35 ( 7 )   2013.7

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    DOI: 10.1007/s10529-013-1183-9

  • TIGHTLY CONTROLLED TETRACYCLINE-INDUCIBLE TRANSCRIPTION SYSTEM FOR EXPLOSIVE GENE EXPRESSION IN CULTURED SILKWORM CELLS Reviewed International journal

    Tsuneyuki Tatsuke, JAE MAN LEE, takahiro kusakabe, Kazuhiro Iiyama, Hideki Sezutsu, Keiro Uchino

    ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY   82 ( 4 )   173 - 182   2013.4

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    DOI: 10.1002/arch.21083

  • Soaking RNAi-mediated modification of Sf9 cells for baculovirus expression system by ectopic expression of Caenorhabditis elegans SID-1 Reviewed International journal

    Applied Microbiology and Biotechnology   2013.3

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  • Coexpression of Escherichia coli RNase III in silkworm cells improves the efficiency of RNA interference induced by long hairpin dsRNAs Reviewed International journal

    Insect Science   20 ( 1 )   69 - 77   2013.2

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    DOI: 10.1111/j.1744-7917.2012.01569.x

  • Comparison of signal peptides for efficient protein secretion in the baculovirus-silkworm system Reviewed International journal

    Central European Journal of Biology   8 ( 1 )   1 - 7   2013.1

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    DOI: 10.2478/s11535-012-0112-6

  • Cell Cycle-Dependent Recruitment of Polycomb Proteins to the ASNS Promoter Counteracts C/ebp-Mediated Transcriptional Activation in Bombyx mori Reviewed International journal

    PloS one   8 ( 1 )   e52320   2013.1

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    DOI: 10.1371/journal.pone.0052320

  • PRODUCTION OF SMALL ANTIBACTERIAL PEPTIDES USING SILKWORM-BACULOVIRUS PROTEIN EXPRESSION SYSTEM Reviewed International journal

    Preparative Biochemistry and Biotechnology   2013.1

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  • Testis-specific Cell Adhesion Molecule, CEACAM6-L, Forms Homophilic Interaction at the Cell Adhesion Site in Vitro Reviewed International journal

    Hitoshi Kurio, JAE MAN LEE, takahiro kusakabe, hiroshi iida

    Zoological science   29 ( 11 )   786 - 793   2012.11

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    DOI: 10.2108/zsj.29.786

  • Production and characterization of the celery mismatch endonuclease CEL II using baculovirus/silkworm expression system Reviewed International journal

    Applied microbiology and biotechnology   2012.11

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  • Establishment of a Bombyx mori nucleopolyhedrovirus (BmNPV) hyper-sensitive cell line from the silkworm e21 strain Reviewed International journal

    Biotechnology letters   34 ( 10 )   1773 - 1779   2012.10

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    DOI: 10.1007/s10529-012-0971-y

  • RNA Interference Induction by Long Hairpin dsRNAs Expressed from Chromosomal DNA of Bombyx mori Cells International journal

    Journal of the Faculty of Agriculture, Kyushu University   57 ( 2 )   441 - 445   2012.9

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    Repository Public URL: http://hdl.handle.net/2324/25203

  • Molecular cloning of BmTUDOR-SN and analysis of its role in RNAi pathway in the silkworm, Bombyx mori (Lepidoptera: Bombycidae). Reviewed International journal

    Zhu, L., Tatsuke, T., Li, Z., Mon, H., Xu, Z., Lee, J.M., and Kusakabe T.,

    Appl. Entomol. Zool.   2012.6

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  • Bombyx mori strains useful for efficient recombinant protein production using a baculovirus vector. Reviewed International journal

    Lee, J.M., Mon, H., Banno, Y., Iiyama, K., Kusakabe, T.

    J. Biotech. Biomater.   2012.6

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  • Monoubiquitination-Dependent Chromatin Loading of FancD2 in Silkworms, a Species Lacking the FA Core Complex. Reviewed International journal

    Sugahara, R., Mon, H., Lee, J.M., and Kusakabe, T.,

    Gene   2012.5

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  • Expression of glycosylated mucin-like domain using baculovirus expression system in silkworm, Bombyx mori. International journal

    Nagata, Y., Sakashita, K., Imanishi, S., Lee, J.M., and Kuskabe T.

    J. Fac. Agr. Kyushu Univ.,   57   2012.4

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  • Molecular cloning of the silkworm p53R2 homolog gene. International journal

    Fujii, M., Takahashi, M., Mon, H., Tatsuke, T., Lee, J.M, and Kuskabe T.

    J. Fac. Agr. Kyushu Univ.,   57   2012.4

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  • Molecular Characterization of heterochromatin proteins 1a and 1b from the Silkworm, Bombyx mori. Reviewed International journal

    Mitsunobu, H., Izumi, H., Mon, H., Tatsuke, T., Lee, J.M., Kusakabe, T.

    Insect Mol. Biol.   21   2012.1

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  • Genome-wide identification of Polycomb target genes reveals a functional association of Pho with Scm in Bombyx mori. Reviewed International journal

    Li, Z., Cheng, D., Mon, H., Tatsuke, T., Zhu, L., Xu, J., Lee, J.M., Xia, Q., and Kusakabe, T.

    PLoS ONE,   7   2012.1

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  • Effective RNA interference in cultured silkworm cells mediated by overexpression of Caenorhabditis elegans SID-1. Reviewed International journal

    Mon, H., Kobayashi, I., Ohkubo, S. Tomita, S., Lee, J.M., Sezutsu, H., Tamura, T., Kusakabe, T.

    RNA Biol.,   9   2012.1

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  • Double-strand breaks repair by gene conversion in silkworm holocentric chromosomes Reviewed International journal

    Mon, H., Lee, J.M., Kawaguchi, Y., Kusakabe, T.

    Mol. Genet. Genomics   2011.9

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  • Post-translational modifications of the N-terminal tail of histone H3 in holocentric chromosomes of bombyx mori. Insect Biochem. Reviewed International journal

    Mon, H., Izumi, M., Mitsunobu, H., Tatsuke, T., Iiyama, K., Jikuya, H., Lee, J.M., Kusakabe, T.

    Mol. Genet. Genomics   2011.9

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  • Construction of gene expression systems in insect cell lines using promoters from the silkworm, Bombyx mori. Reviewed International journal

    Lee JM, Takahashi M, Mon H, Mitsunobu H, Koga K, Kawaguchi Y, Nakajima Y, Kusakabe T.

    J Biotechnol   2008.2

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  • Efficient protein expression in Bombyx mori larvae of the strain d17 highly sensitive to B. mori nucleopolyhedrovirus. Reviewed International journal

    Kawakami, N., Lee, J.M., Mon, H., Kubo, Y., Banno, Y., Kawaguchi, Y., Maenaka, K., Park, E.Y., Koga, K. and Kusakabe, T.

    Mol Biotechnol.   2008.2

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  • Screening of high-permissive silkworm strains for efficient recombinant protein production in Autographa californica nuclear polyhedrosis virus (AcNPV). Reviewed International journal

    Lee J.M., Mon H., Takahashi M., Kawakami N. Yoshida Y., Banno Y., Koga K., Kawaguchi Y. and Kusakabe T.

    J. Insect Biotech. Seric.   2007.3

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  • Efficient gene transfer into silkworm larval tissues by a combination of sonoporation and lipofection. Reviewed International journal

    Lee J.M., Takahashi M., Mon H., Koga K., Kawaguchi Y., and Kusakabe T.

    Cell Biol. Int.   29 ( 11 )   976 - 979   2005.1

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    DOI: 10.1016/j.cellbi.2005.07.007

  • SARS-CoV-2 strain-specific anti-Spike IgG ELISA utilizing spike protein produced by silkworms Reviewed

    @Takeyuki Goto, @Tomoki Sasaki, @Yong Chong, @Masahiro Taniguchi, @Jae Man Lee, @Akitsu Masuda, #Takeru Ebihara, @Kenichiro Shiraishi, @Naoki Tani, @Akiko Yonekawa, @Kei Gondo, @Hiroyuki Kuwano, @Nobuyuki Shimono, @Hideyuki Ikematsu, @Koichi Akashi, @Takahiro Kusakabe

    Human Antibodies   31 ( 3 )   27 - 33   2023.10

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    DOI: 10.3233/HAB-230006

  • Transdermal Transmission Blocking Vaccine for Malaria using a Solid-in-Oil Dispersion Reviewed International journal

    #Keisuke Tanaka, @Kosuke Minamihata, @Rie Wakabayashi, @Jae Man Lee, @Takeshi Miyata, @Takahiro Kusakabe, @Noriho Kamiya, @Masahiro Goto

    Journal of Pharmaceutical Sciences   112 ( 2 )   411 - 415   2023.1

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    DOI: https://doi.org/10.1016/j.xphs.2022.10.031

  • TRPC3-Nox2 Protein Complex Formation Increases the Risk of SARS-CoV-2 Spike Protein-Induced Cardiomyocyte Dysfunction through ACE2 Upregulation Reviewed

    @Yuri Kato, @Kazuhiro Nishiyama, @Jae Man Lee, @Yuko Ibuki, @Yumiko Imai, @Takamasa Noda, @Noriho Kamiya, @Takahiro Kusakabe, @Yasunari Kanda, @Motohiro Nishida

    International Journal of Molecular Sciences   24 ( 1 )   102 - 102   2023.1

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    DOI: https://doi.org/10.3390/ijms24010102

  • Construction of gene co-expression networks in cultured silkworm cells and identification of previously uncharacterized lepidopteran-specific genes required for chromosome dynamics. Reviewed

    @Mon H, @Sato M, @Lee JM, @Kusakabe T.

    Insect Biochemistry and Molecular Biology   151   103875 - 103875   2022.12

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    DOI: 10.1016/j.ibmb.2022.103875

  • Characterization of a Novel Heterochromatin Protein 1 Homolog "HP1c" in the Silkworm, Bombyx mori Reviewed

    @Hino M, @Tatsuke T, #Morio A, @Mon H, @Lee JM, @Masuda A, #Kakino K, #Tonooka Y, @Kusakabe T

    Insects   13 ( 7 )   631   2022.7

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    DOI: https://doi.org/10.3390/insects13070631

  • Silkworm FoxL21 plays important roles as a regulator of ovarian development in both oogenesis and ovariole development Reviewed

    #Tanaka M, @Fujii T, @Mon H, @Lee JM, #Kakino K, @Fukumori H, #Ebihara T, #Nagasato T, @Hino M, #Tonooka Y, #Moriyama T, @Fujita R, @Banno Y, @Kusakabe T.

    Insect Biochemistry and Molecular Biology   143   103737   2022.4

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    DOI: 10.1016/j.ibmb.2022.103737

  • Non-molting dwarf (nm-d) as a mutant of Bombyx mori with a defect in purine synthesis Reviewed

    @Fujii T, #Kakino K, @Fukumori H, @Hino M, @Lee JM, @Kusakabe T, @Banno Y.

    Insect Biochemistry and Molecular Biology   138   2021.11

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    DOI: 10.1016/j.ibmb.2021.103636

  • Functional binding of E-selectin to its ligands is enhanced by structural features beyond its lectin domain Reviewed

    Fajr A. Aleisa, Kosuke Sakashita, Jae Man Lee, Dina B. AbuSamra, Bader Al Alwan, Shuho Nozue, Muhammad Tehseen, Samir M. Hamdan, X. Satoshi Habuchi, X. Takahiro Kusakabe, Jasmeen S. Merzaban

    Journal of Biological Chemistry   295 ( 11 )   3719 - 3733   2020.3

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    DOI: 10.1074/jbc.RA119.010910

  • Mild inactivation and inhibition of phenoloxidase in silkworm serum for xeno-free culture of insect cells Reviewed

    Masato Hino, Noriko Karasaki, Tsuneyuki Tatsuke, Daisuke Morokuma, Akitsu Masuda, Hiroaki Mon, Jae Man Lee, Takahiro Kusakabe

    Journal of Insect Biotechnology and Sericology   89 ( 1 )   9 - 16   2020.1

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    DOI: 10.11416/jibs.89.1_009

  • Characterization of the RAGE-binding protein, Strongyloides venestatin, produced by the silkworm-baculovirus expression system Reviewed

    Daigo Tsubokawa, Jae Man Lee, Takeshi Hatta, Fusako Mikami, Haruhiko Maruyama, Takeshi Arakawa, Takahiro Kusakabe, Naotoshi Tsuji

    Infection, Genetics and Evolution   75   2019.11

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    DOI: 10.1016/j.meegid.2019.103964

  • Heterologous Production and Glycosylation of Japanese Eel Follitropin Using Silkworm Reviewed

    Sun Mee Hong, Ji Hyun Choi, Sun Jung Jo, Kwan Sik Min, Dae Jung Kim, Jae Man Lee, Takahiro Kusakabe

    Biotechnology and Bioprocess Engineering   24 ( 5 )   745 - 753   2019.9

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    DOI: 10.1007/s12257-019-0045-2

  • Polymerization of Horseradish Peroxidase by a Laccase-Catalyzed Tyrosine Coupling Reaction Reviewed

    Dani Permana, Kosuke Minamihata, Tsuneyuki Tatsuke, Jae M. Lee, Takahiro Kusakabe, Masahiro Goto, Noriho Kamiya

    Biotechnology Journal   14 ( 6 )   2019.6

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    DOI: 10.1002/biot.201800531

  • Identification of an insecticidal toxin produced by Enterobacter sp. strain 532 isolated from diseased Bombyx mori silkworms Reviewed

    Mai Morishita, Akitsu Masuda, Hiroaki Mon, Man Lee, Takahiro Kusakabe, Kosuke Tashiro, Chisa Yasunaga-Aoki, Kazuhiro Iiyama

    FEMS microbiology letters   366 ( 2 )   2019.1

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    DOI: 10.1093/femsle/fny295

  • Toxin complex is a major virulence determinant of Enterobacter sp. 532 against the silkworm, Bombyx mori Reviewed

    Journal of Insect Biotechnology and Sericology   88 ( 1 )   1_021 - 1_025   2019.1

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    DOI: 10.11416/jibs.88.1_021

  • Gene structure and cDNA sequence of 2-Cys peroxiredoxin in the harmful algal bloom species Chattonella marina and its gene transcription under different light intensities Reviewed

    Koki Mukai, Ayano Teramoto, Xuchun Qiu, Yohei Shimasaki, Yoko Kato-Unoki, Man Lee, Naohiro Mizoguchi, Mst Ruhina Margia Khanam, Hina Satone, Tsuneyuki Tatsuke, Takahiro Kusakabe, Yuji Oshima

    European Journal of Phycology   53 ( 1 )   29 - 38   2018.1

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    DOI: 10.1080/09670262.2017.1346206

  • SUMOylation regulates the localization and activity of Polo-like kinase 1 during cell cycle in the silkworm, Bombyx mori Reviewed

    Zhiqing Li, Qixin Cui, Jian Xu, Daojun Cheng, Xiaoyan Wang, Bingqian Li, Man Lee, Qingyou Xia, Takahiro Kusakabe, Ping Zhao

    Scientific Reports   7 ( 1 )   2017.12

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    DOI: 10.1038/s41598-017-15884-7

  • Molecular characterization of mitochondrial Zucchini and its relation to nuage-piRNA pathway components in Bombyx mori ovary-derived BmN4 cells Reviewed

    Anandrao Ashok Patil, Tsuneyuki Tatsuke, Hiroaki Mon, Man Lee, Daisuke Morokuma, Masato Hino, Takahiro Kusakabe

    Biochemical and Biophysical Research Communications   493 ( 2 )   971 - 978   2017.11

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    DOI: 10.1016/j.bbrc.2017.09.107

  • Lipidation of BmAtg8 is required for autophagic degradation of p62 bodies containing ubiquitinated proteins in the silkworm, Bombyx mori Reviewed

    Ming Ming Ji, Man Lee, Hiroaki Mon, Kazuhiro Iiyama, Tsuneyuki Tatsuke, Daisuke Morokuma, Masato Hino, Mami Yamashita, Kazuma Hirata, Takahiro Kusakabe

    Insect Biochemistry and Molecular Biology   89   86 - 96   2017.10

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    DOI: 10.1016/j.ibmb.2017.08.006

  • Characterization of Armitage and Yb containing granules and their relationship to nuage in ovary-derived cultured silkworm cell Reviewed

    Anandrao Ashok Patil, Tsuneyuki Tatsuke, Hiroaki Mon, Man Lee, Daisuke Morokuma, Masato Hino, Takahiro Kusakabe

    Biochemical and Biophysical Research Communications   490 ( 2 )   134 - 140   2017.8

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    DOI: 10.1016/j.bbrc.2017.06.008

  • Identification and functional analysis of outer kinetochore genes in the holocentric insect Bombyx mori Reviewed

    Hiroaki Mon, Man Lee, Masanao Sato, Takahiro Kusakabe

    Insect Biochemistry and Molecular Biology   86   1 - 8   2017.7

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    DOI: 10.1016/j.ibmb.2017.04.005

  • Alkaline protease contributes to pyocyanin production in Pseudomonas aeruginosa Reviewed

    Kazuhiro Iiyama, Eigo Takahashi, Man Lee, Hiroaki Mon, Mai Morishita, Takahiro Kusakabe, Chisa Yasunaga-Aoki

    FEMS Microbiology Letters   364 ( 7 )   2017.4

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    DOI: 10.1093/femsle/fnx051

  • Tetrodotoxin- and tributyltin-binding abilities of recombinant pufferfish saxitoxin and tetrodotoxin binding proteins of Takifugu rubripes Reviewed International journal

    Hina Satone, Shohei Nonaka, JAE MAN LEE, Yohei Shimasaki, takahiro kusakabe, Shun-ichiro Kawabata, Yuji Oshima

    TOXICON   125   50 - 52   2017.1

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    DOI: 10.1016/j.toxicon.2016.11.245

  • A reconsideration of the taxonomic position of two bacterial strains isolated from Flacherie-Diseased silkworms in 1965 Reviewed

    Kazuhiro Iiyama, Mai Morishita, Man Lee, Hiroaki Mon, Takahiro Kusakabe, Kosuke Tashiro, Taiki Akasaka, Chisa Yasunaga-Aoki, Kazuhisa Miyamoto

    Journal of Insect Biotechnology and Sericology   86 ( 2 )   35 - 41   2017.1

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    DOI: 10.11416/jibs.86.2_035

  • In vitro screening for inhibitor of cloned Drosophila melanogaster tyramine-β-hydroxylase and docking studies Reviewed International journal

    93   889 - 895   2016.12

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  • In vitro screening for inhibitor of cloned Drosophila melanogaster tyramine-β-hydroxylase and docking studies Reviewed

    93   889 - 895   2016.12

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    Biogenic amines are common biologically active substances extended within the whole animal kingdom where they play vital roles as signal transducer as well as regulator of cell functions. One of these biogenic amines called octopamine (OA) is synthesized from tyramine (TA) by the catalysis of tyramine-β-hydroxylase (TβH) originated in the insect nervous system. Both TA and OA act as neurotransmitters, neurohormones and neuromodulators in the arthropod nervous system. Herein, the inhibitory activity of 1-arylimidazole-2(3H)-thiones (AITs) was tested on cloned Drosophila tyramine-β-hydroxylase (DmTβH) expressed in Bombyx mori strain. Radiolabelled 3H-TA was used to analyze the activity of AITs exhibited inhibitory effects on DmTβH, whose ID50 values range from 0.02 to 2511 nM where DmTβH was inhibited in a dose-dependent manner at pH 7.6 and 25 °C during a 30 min of incubation. To understand the catalytic role of the TβH, a three dimensional structure of the TβH from Drosophila melanogaster was constructed by homology modeling using the Phyre2 web server with 100% confidence. The modeled three-dimensional structure of TβH was used to perform the docking study with AITs. This may give more insights to precise design of inhibitors for TβH to control insect's population.

    DOI: 10.1016/j.ijbiomac.2016.06.026

  • Virulence of lipopolysaccharide-deficient mutants of Serratia liquefaciens toward the silkworm, Bombyx mori. Reviewed International journal

    Journal of Insect Biotechnology and Sericology   85   7 - 14   2016.4

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    DOI: 10.11416/jibs.85.1_007

  • Effect of antibiotics on extracellular protein level in Pseudomonas aeruginosa. Reviewed International journal

    Plasmid   84-85   44 - 50   2016.3

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    DOI: 10.1016/j.plasmid.2016.03.001

  • Characterization of the roles of DNA polymerases, clamp, and clamp loaders during s-phase progression and cell cycle regulation in the silkworm, bombyx mori Reviewed

    Masato Hino, Daisuke Morokuma, Hiroaki Mon, Jae Man Lee, Takahiro Kusakabe

    Journal of Insect Biotechnology and Sericology   85 ( 2 )   21 - 29   2016.1

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    DOI: 10.11416/jibs.85.2_021

  • CRISPR/Cas9-mediated knockout of factors in non-homologous end joining pathway enhances gene targeting in silkworm cells Invited Reviewed International journal

    SCIENTIFIC REPORTS   5   2015.12

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    DOI: 10.1038/srep18103

  • Loqs depends on R2D2 to localize in D2 body-like granules and functions in RNAi pathways in silkworm cells Reviewed International journal

    Insect biochemistry and molecular biology   64   78 - 90   2015.9

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  • Expression of Recombinant Viscum Album Coloratum lectin B-chain in the Silkworm Expression System and Evaluation of Antioxidant Activity Reviewed International journal

    Sun Mee Hong, Ji-Hyun Choi, Sun-Jung Jo, Yohei Shimasaki, Seong Kyu Song, JAE MAN LEE, takahiro kusakabe

    BIOTECHNOLOGY AND BIOPROCESS ENGINEERING   20 ( 3 )   515 - 522   2015.6

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    DOI: 10.1007/s12257-014-0806-x

  • Biochemical characterization of maintenance DNA methyltransferase DNMT-1 from silkworm, Bombyx mori

    INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY   58   55 - 65   2015.3

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    DOI: 10.1016/j.ibmb.2015.01.008

  • Biochemical characterization of maintenance DNA methyltransferase DNMT-1 from silkworm, Bombyx mori Reviewed International journal

    INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY   58   55 - 65   2015.3

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    DOI: 10.1016/j.ibmb.2015.01.008

  • Dynamics of polycomb proteins-mediated histone modifications during UV irradiation-induced DNA damage

    INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY   55   9 - 18   2014.12

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    DOI: 10.1016/j.ibmb.2014.10.001

  • Differential contribution of the Fanconi anemia-related proteins to repair of several types of DNA damage in cultured silkworm cells

    FEBS LETTERS   588 ( 21 )   3959 - 3963   2014.11

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    DOI: 10.1016/j.febslet.2014.09.009

  • Characterization of cryptopygus antarcticus endo-β-1, 4-glucanase from Bombyx mori expression systems Reviewed International journal

    56 ( 10 )   878 - 889   2014.10

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  • A conserved SUMOylation signaling for cell cycle control in a holocentric species Bombyx mori

    INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY   51   71 - 79   2014.8

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    DOI: 10.1016/j.ibmb.2014.05.008

  • Draft genome sequence of entomopathogenic Serratia liquefaciens strain FK01.

    Genome Announcements   2 ( 3 )   e00609 - e00614   2014.6

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  • Characterization of KfrA proteins encoded by a plasmid of Paenibacillus popilliae ATCC 14706 T Reviewed International journal

    Meta gene   4   29 - 44   2014.6

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  • Characterization of KfrA proteins encoded by a plasmid of Paenibacillus popilliae ATCC 14706 T Reviewed International journal

    Meta gene   4   29 - 44   2014.6

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  • Phylogenetic relationship of Paenibacillus species based on putative replication origin regions and analysis of an yheCD-like sequence found in this region

    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY   78 ( 5 )   891 - 897   2014.5

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    DOI: 10.1080/09168451.2014.905188

  • Expression, purification, and characterization of endo-β-N-acetylglucosaminidase H using baculovirus-mediated silkworm protein expression system

    Takumi Mitsudome, Jian Xu, Yudai Nagata, Atsushi Masuda, Kazuhiro Iiyama, Daisuke Morokuma, Zhiqing Li,, 門 宏明, JAE MAN LEE, takahiro kusakabe

    172 ( 8 )   3978 - 3988   2014.4

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  • Middle region of FancM interacts with Mhf and Rmi1 in silkworms, a species lacking the Fanconi anaemia (FA) core complex

    INSECT MOLECULAR BIOLOGY   23 ( 2 )   185 - 198   2014.4

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    DOI: 10.1111/imb.12072

  • Establishment of Caenorhabditis elegans SID-1-Dependent DNA Delivery System in Cultured Silkworm Cells Reviewed International journal

    MOLECULAR BIOTECHNOLOGY   56 ( 3 )   2014.3

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    DOI: 10.1007/s12033-013-9694-0

  • Roles of Piwi Proteins in Transcriptional Regulation Mediated by HP1s in Cultured Silkworm Cells

    PLOS ONE   9 ( 3 )   e92313   2014.3

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    DOI: 10.1371/journal.pone.0092313

  • Chromatin-induced spindle assembly plays an important role in metaphase congression of silkworm holocentric chromosomes Reviewed International journal

    Insect biochemistry and molecular biology   45   40 - 50   2014.2

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  • Establishment of a soaking RNA interference and Bombyx mori nucleopolyhedrovirus (BmNPV)-hypersensitive cell line using Bme21 cell Reviewed International journal

    APPLIED MICROBIOLOGY AND BIOTECHNOLOGY   97 ( 24 )   2013.12

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    DOI: 10.1007/s00253-013-5279-x

  • Characterization of Tudor-sn-containing granules in the silkworm, Bombyx mori Reviewed International journal

    ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY   43 ( 8 )   2013.8

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    DOI: 10.1016/j.ibmb.2013.04.004

  • Characterization, Localization, and Stage-Dependent Gene Expression of Gonadotropin Receptors in Chub Mackerel (Scomber japonicus) Ovarian Follicles

    BIOLOGY OF REPRODUCTION   88 ( 6 )   2013.6

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    DOI: 10.1095/biolreprod.112.107292

  • AMINO ACID DEPRIVATION-INDUCED EXPRESSION OF ASPARAGINE SYNTHETASE REGULATES THE GROWTH AND SURVIVAL OF CULTURED SILKWORM CELLS Reviewed International journal

    ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY   83 ( 2 )   2013.6

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    DOI: 10.1002/arch.21091

  • A MC motif in silkworm Argonaute 1 is indispensible for translation repression Reviewed International journal

    INSECT MOLECULAR BIOLOGY   22 ( 3 )   2013.6

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    DOI: 10.1111/imb.12023

  • Characterization and recombinant protein expression of ferritin light chain homologue in the silkworm, Bombyx mori Reviewed International journal

    Insect Science   21 ( 2 )   2013.4

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    DOI: 10.1111/1744-7917.12031

  • Development and characterization of a new Bombyx mori cell line for protein expression Reviewed International journal

    Arun M Khurad, Ravindra S Bahekar, Min-Juan Zhang, Ashish D Tiple, JAE MAN LEE, Chuan-Xi Zhang, takahiro kusakabe

    Journal of Asia-Pacific Entomology   16 ( 1 )   17 - 22   2013.3

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  • Phylogenetic analysis of Paenibacillus popilliae and its related taxa based on housekeeping genes. Reviewed International journal

    J. Insect Biotech. Seric.,   82   2013.2

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    DOI: 10.11416/jibs.82.1_001

  • Genome-wide identification of Argonaute 1- and Argonaute 2-regulating genes revealed an inhibition of macula-like virus by RNAi pathway in the silkworm, Bombyx mori Reviewed International journal

    J. Insect Biotech. Seric.,   82   2013.2

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    DOI: 10.11416/jibs.82.1_019

  • Expression and characterization of a recombinant Drosophila tyramine-β-hydroxylase in silkworm infected with recombinant baculovirus Reviewed International journal

    15 ( 4 )   567 - 572   2012.6

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    DOI: http://dx.doi.org/10.1016/j.aspen.2012.06.004

  • Identification and characterization of Polycomb group genes in the silkworm, Bombyx mori. Reviewed International journal

    Li, Z., Tatsuke, T., Sakashita, K., Zhu, L., Xu, J., Mon, H., Lee, J.M., and Kusakabe, T.

    Mol. Biol. Report,   39   2012.1

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  • Improvement in pHERD vectors to express recombinant proteins tagged with hexahistidine at either the NH2- or COOH- terrminal in Pseudomonas aeruginosa. Reviewed International journal

    Iiyama, K., Lee, J.M., Kusakabe, T., Yasunaga-Aoki, C., and Shimizu, S.

    J. Insect Biotech. Seric.,   80   2011.10

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  • Virulence of an exotoxin A-deficient strain of Pseudomonas aeruginosa toward the silkworm, Bombyx mori. Reviewed International journal

    Chieda, Y., Iiyama, K., Lee, J.M., Kusakabe, T., Yasunaga-Aoki, C., and Shimizu, S.

    Microbial. Pathogenesis,   51   2011.10

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  • Molecular Characterization of heterochromatin proteins 1a and 1b from the Silkworm, Bombyx mori. Reviewed International journal

    Mitsunobu, H., Izumi, H., Mon, H., Tatsuke, T., Lee, J.M., Kusakabe, T.

    Insect Mol. Biol.   2011.4

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  • Tributyltin-binding protein type 1, a lipocalin, prevents inhibition of osteoblastic activity by tributyltin in fish scales. Reviewed International journal

    Satone, H., Lee, J.M., Oba, Y., Kusakabe, T., Akahoshi, E., Miki, S., Suzuki, N., Sasayama, Y., Nassef, M., Shimasaki, Y., Kawabata, S., Honjo, T., Oshima, Y.

    Aquat. Toxicol.   103   2011.2

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  • Molecular Characterization of Core Histones in the Silkworm, Bombyx mori. Reviewed International journal

    Mitsunobu, H., Izumi, M., Iiyama, K., Jikuya, H., Lee, J.M., Mon, H., Kawaguchi, Y., Kusakabe, T.

    J. Insect Biotech. Seric.   79   2010.10

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  • dsRNA Binding Activity of Silkworm Larval Hemolymph is Mediated by Lipophorin Complex

    Sakashita, K., Tatsuke, T., Masaki, Y., Lee, J., Kawaguchi, Y., Kusakabe, T.

    J. Fac. Agr., Kyushu Univ.   2010.4

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  • Efficient Soluble Protein Production on Transgenic Silkworms Expressing Cytoplasmic Chaperones using Baculovirus Expression System. Reviewed International journal

    Hong, S.M., Yamashita, J., Mitsunobu, H., Uchino, K., Kobayashi, I., Tamura, T., Nakajima, H., Miyagawa, H., Lee, J.M., Mon, H., Miyata, T., Kawaguchi, Y., Kusakabe, T.

    Appl. Microbiol. Biotech.   2010.4

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  • The telomere-specific non-LTR retrotransposons SART1 and TRAS1 are suppressed by Piwi subfamily proteins in the silkworm, Bombyx mori. Reviewed International journal

    Tatsuke, T., Sakashita, K., Masaki, Y., Lee, J.M., Kawaguchi, Y., Kusakabe, T.

    Cell. Mol. Biol. Lett.   15   2010.4

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  • Analysis of protein interactions with two-hybrid system in cultured insect cells. Reviewed International journal

    Mon, H., Sugahara, R., Hong, S.M., Lee, J.M., Kamachi, Y., Kawaguchi, Y., Kusakabe, T.

    Anal. Biochem.   2009.9

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  • Construction of piggyBac-based Vectors Using Visible and Drug-resistance Marker for Introducing Foreign Genes into Silkworm Cultured Cells.

    Tatsuke, T., Hong, S.M., Tobata, H., Mon, H., Lee, J.M., Kawaguchi, Y., Kusakabe, T.

    J. Fac. Agr., Kyushu Univ.   54   2009.4

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    Repository Public URL: http://hdl.handle.net/2324/16121

  • Insecticidal bacterium isolated from an ant lion larva from Munakata, Japan. Reviewed International journal

    Egami, I., Iiyama, K., Zhang, P., Chieda, Y., Ino, N., Hasegawa, K., Lee, J.M., Kusakabe, T., Yasunaga-Aoki, C. and Shimizu, S.,

    J. Appl. Entomol.   2009.3

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  • Establishment of Tetracycline-inducible Gene Expression Systems in the Silkworm, Bombyx mori Reviewed International journal

    Karasaki, N., Mon, M., Takahashi, M., Lee, JM., Koga, K., Kawaguchi, Y., and Kusakabe, T.

    Biotechnol. Lett.   2009.3

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  • Sequence-nonspecific suppression of gene expression by double-stranded RNA in silkworm cultured cells Reviewed International journal

    Sakashita, K., Tatsuke, T., Lee, J.M. Kawaguchi, Y., and Kusakabe, T.

    J. Insect Biotech. Seric.   2009.2

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  • Singular compound eye architecture of the varnished eye mutation, ve, in Bombyx mori. Reviewed International journal

    Kawaguchi, Y., Tatsuke, T., Kusakabe, T., Lee, J.M. and Koga, K.

    J. Insect Biotech. Seric.   2008.2

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  • Structure and expression analysis of the cecropin-E gene from the silkworm, Bombyx mori. Reviewed International journal

    Hong, S.M., Kusakabe, T., Lee, J.M., Tatsuke, T., Kawaguchi, Y., Kang, M.W., Kang, S.W., Kim, K.A. and Nho, S.K.

    Biosci Biotechnol Biochem.   2008.2

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  • Inactivation of pyocyanin synthesis genes has no effect on the virulence of Pseudomonas aeruginosa PAO1 toward the silkworm, Bombyx mori. Reviewed International journal

    Chieda, Y., Iiyama, K., Lee, J.M., Kusakabe, T., Yasunaga-Aoki, C. and Shimizu, S.

    FEMS Microbiol Lett.   2008.2

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  • Fertility and hatching of the vit mutant eggs in Bombyx mori. Reviewed International journal

    Kawaguchi, Y., Tatsuke, T., Oike, Y., Kusakabe, T., Lee, J.M. and Koga, K.

    J. Insect Biotech. Seric.   2008.2

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  • Virulence of phospholipase C mutant of Pseudomonas aeruginosa PA01 against the silkworm, Bombyx mori. Reviewed International journal

    Iiyama, K., Chieda, Y., Lee, J.M., Kusakabe, T., Yasunaga-Aoki, C. and Shimizu, S.

    J. Insect Biotech. Seric.   2008.2

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  • Proteomic Profiling of the Silkworm Skeletal Muscle Proteins during Larval-Pupal Metamorphosis. Reviewed International journal

    Zhang P., Aso Y., Jikuya H., Kusakabe T., Lee JM., Kawaguchi Y., Yamamoto K., Banno Y and Fujii H.

    J Proteome Res.   2007.7

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  • Characteristic of Se, the white-sided egg mutation in Bombyx mori. Reviewed International journal

    Kawaguchi Y., Yoshida Y., Kusakabe T., Lee JM., and Koga K.

    J. Insect Biotech. Seric.   2007.6

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  • Effect of inactivation of the superoxide dismutase genes on the virulence of Pseudomonas aeruginosa PAO1 to silkworm, Bombyx mori. Reviewed International journal

    Iiyama K., ChiedaY., Lee J.M., Kusakabe T., Yasunaga-Aoki C. and Shimizu S.

    Appl. Environ. Microbiol.   2007.3

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  • Effect of inactivation of the superoxide dismutase genes on the virulence of Pseudomonas aeruginosa PAO1 to silkworm, Bombyx mori. Reviewed International journal

    Iiyama,K., Chieda,Y., Lee, JM., Kusakabe, T., Yasunaga-Aoki, C., Lee, J., Kusakabe, T., Shimizu, S.

    Appl. Environ. Microbiol.   2007.3

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  • Molecular Cloning of Silkworm Cdc37 and its Interaction with Hsp90 Chaperon. Reviewed International journal

    Yamashita J., Miyagawa Y., Sugahara R., Mon H., Mitsunobu H., Lee J.M., Kawaguchi Y.and Kusakabe T.

    J. Insect Biotech. Seric.   2007.3

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  • Heterotrimeric Complex of Replication Protein A, a Single-Stranded DNABinding Protein, from the Silkworm, Bombyx mori. Reviewed International journal

    Sugahara R., Mon H., Yamashita J., Mitsunobu H., Lee J.M., Kawaguchi Y., Koga K. and Kusakabe T.

    J. Insect Biotech. Seric.   2007.3

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  • Radiation resistance and its inheritance in the silkworm, Bombyx mori.

    Takahashi M., Lee J.M., Mon H., Yoshida H., Kawaguchi Y., Maekawa H., Koga K., and Kusakabe T.

    J. Fac. Agr., Kyushu Univ.   2006.1

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  • Role of the Silkworm Argonaute2 Homologue Gene in Double-Strand Break Repair of Extrachromosomal DNA. Reviewed International journal

    Tsukioka H., Takahashi M., Mon H., Okano K., Mita K., Shimada T., Lee J.M., Kawaguchi Y., Koga K., and Kusakabe T.

    Nucleic Acids Res.   2006.1

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  • Construction of gateway-based destination vectors for detecting subcellular localization of proteins in the silkworm, Bombyx mori. Reviewed International journal

    Mitsunobu H., Sakashita K., Mon H., Lee J.M., Kawaguchi Y., Koga K., and Kusakabe T.

    J. Insect Biotech. Seric.   2006.1

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  • Cell Cycle Arrest induced by Radiation in Cultured Silkworm Cells. Reviewed International journal

    Takahashi M., Lee J.M., Mon H., Kawaguchi Y., Koga K., and Kusakabe T.

    J. Insect Biotech. Seric.   2006.1

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  • Micropylar structure of chorion of the female sterile mutation, bd, in, Bombyx mori. Reviewed International journal

    Kawaguchi Y., Kusakabe T., Lee J.M., Nakajima Y., and Koga K.

    J. Insect Biotech. Seric.   2006.1

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  • Efficient nonviral gene transfer mediated by polyethylenimine in an insect cell line.

    Maeda, T., Kusakabe, T., Lee, J.M., Miyagawa, Y., Kawaguchi, Y. and Koga, K.

    J. Insect Biotech. Seric.   2005.1

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  • Differential expression of a Bombyx mori AHA1 homologue during spermatogenesis. International journal

    Miyagawa, Y., Lee, J.M., Maeda, T., Koga, K., Kawaguchi, Y. and Kusakabe, T.

    Insect Mol. Biol.   14 ( 3 )   245 - 253   2005.1

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    DOI: 10.1111/j.1365-2583.2004.00553.x

  • Pathogenicity of gacA mutant of Pseudomonas aeruginosa PA01 in the silkworm, Bombyx mori. International journal

    Chieda, Y., Iiyama, K., Yasunaga-Aoki, C., Lee, J.M., Kusakabe, T. and Shimizu, S.

    FEMS Microbiol. Lett.   244 ( 1 )   181 - 186   2005.1

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    DOI: 10.1016/j.femsle.2005.01.032

  • Cloning and characterization of a ribonuclease L inhibitor from the silkworm, Bombyx mori. International journal

    Maeda, T., Lee, J.M., Miyagawa, Y., Koga, K., Kawaguchi, Y. and Kusakabe, T.

    DNA Sequence   16 ( 1 )   21 - 27   2005.1

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    DOI: 10.1080/10425170400028871

  • In vivo DNA double-strand breaks enhance gene targeting in cultured silkworm cells. International journal

    Mon, H., Kusakabe, T., Lee, J.M., Kawaguchi, Y. and Koga, K.

    Comp. Biochem. Phys. Part B   139 ( 1 )   99 - 106   2004.1

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    DOI: 10.1016/j.cbpc.2004.06.013

  • Isolation and characterization of differently expressed cDNAs in a meiotic recombination strain of Bombyx mori.

    Miyagawa, Y., Kusakabe, T., Lee, J.M., Maeda, T., Kawaguchi, Y. and Koga, K.

    J. Insect Biotech. Seric.   2004.1

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  • Molecular cloning and characterization of the translationally controlled tumor protein (TCTP) gene in Bombyx mori. International journal

    Lee, J.M., Kusakabe, T., Kawaguchi, Y., Takahashi, M., Mon, H., Nho, S.K. and Koga, K.

    Comp. Biochem. Phys. Part B   139 ( 1 )   35 - 43   2004.1

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    DOI: 10.1016/j.cbpc.2004.06.004

  • Phylogeny of Bombyx mandarina inhabiting Korea analysing the isozyme and hemolymph protein polymorphism.

    Lee, J.M., Kim, K.A. and Nho, S.K.

    Korean J. Seri. Sci.   2003.1

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    Language:Others  

  • Molecular characterization of Heat Shock Cognate 70-4 promoter from the silkworm, Bombyx mori

    Lee, J.M., Kusakabe, T., Kawaguchi, Y., Aoki, C., Nho, S.K., Nakajima, Y. and Koga, K.

    J. Insect Biotech. Seric.   2003.1

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    Language:English  

  • Phylogenetic relationship and characterization of Korean native silkworm strains based on RAPDs and isozyme analysis, Bombyx mori .

    Lee, J.M. and Nho, S.K.

    Korean J. Seri. Sci.   2001.1

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    Language:Others  

  • Characteristics of Korean native strains in the domesticated silkworm, Bombyx mori.

    Nho, S.K. and Lee, J.M.

    Korean J. Seri. Sci.   2000.1

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    Language:Others  

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Presentations

  • カイコにおける機能未知遺伝子KWMTBOMO08400の機能解析

    #米澤徳隆・@柿野耕平・@門 宏明・@李 在萬・@日下部宜宏

    日本蚕糸学会第94回大会  2024.3 

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    Event date: 2024.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:つくば市   Country:Japan  

    Other Link: https://sites.google.com/view/jsss-tsukuba-2024/

  • ヒトノロウイルスVLPを形成するVP1タンパ ク質の不溶性原因の解明と可溶性向上

    #鶴見祐土・@増田亮津・@李 在萬・@門 宏明・@藤田龍介・@日野真人・@藤井 告・@日下部宜宏

    日本蚕糸学会第94回大会  2024.3 

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    Event date: 2024.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:つくば市   Country:Japan  

    Other Link: https://sites.google.com/view/jsss-tsukuba-2024/

  • バキュロウイルス非必須遺伝子クラスター欠損株の構築とタンパク質発現解析

    #山口礼華・#海老原健・@門 宏明・@李 在萬・@藤田龍介・@増田亮津・@日野真人・#渋谷美咲・@日下部宜宏

    日本蚕糸学会第94回大会  2024.3 

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    Event date: 2024.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:つくば市   Country:Japan  

    Other Link: https://sites.google.com/view/jsss-tsukuba-2024/

  • 組換えタンパク質生産性向上の目指したランダム突然変異導入によるカイコバキュロウイルス変異体の獲得

    #渋谷美咲・#海老原健・@門 宏明・@李 在萬・#山口礼華・@増田亮津・@日野真人・@藤田龍介・@日下部宜宏

    日本蚕糸学会第94回大会  2024.3 

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    Event date: 2024.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:つくば市   Country:Japan  

    Other Link: https://sites.google.com/view/jsss-tsukuba-2024/

  • 難発現タンパク質の生産を目的とした分泌性の異なるモデルタンパク質の比較解析

    #海老原健・#谷 菜月・@李 在萬・@門 宏明・@日下部宜宏

    日本蚕糸学会第94回大会  2024.3 

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    Event date: 2024.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:つくば市   Country:Japan  

    Other Link: https://sites.google.com/view/jsss-tsukuba-2024/

  • カイコ培養細胞を用いた新規小胞体ストレス応答遺伝子の探索と機能解析

    #谷 菜月, @門 宏明, #海老原 健, @李 在萬, @藤田 龍介, @日下部 宜宏

    第46回日本分子生物学会年会  2023.12 

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    Event date: 2023.12

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:神戸ポートアイランド   Country:Japan  

  • 鱗翅目昆虫の細胞分裂に関わる機能未知遺伝子の解析

    #米澤 徳隆, @門 宏明, #柿野 耕平, @佐藤 昌直, @藤田 龍介, @李 在萬, @日下部 宜宏

    第46回日本分子生物学会年会  2023.12 

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    Event date: 2023.12

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:神戸ポートアイランド   Country:Japan  

  • カイコの脂肪体におけるBmNPV感染動態の解明

    #中桐 槙也, @門 宏明, #海老原 健, @李 在萬, @藤田 龍介, @日野 真人, @日下部 宜宏

    第46回日本分子生物学会年会  2023.12 

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    Event date: 2023.12

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:神戸ポートアイランド   Country:Japan  

  • カイコ-バキュロウイルス発現系における、プロタンパク質転換システムの改変によるTGF-β成熟の効率化

    #小山茜音, @李在萬, @増田亮津, @門宏明, @藤田龍介, @日下部宣宏

    第45回日本分子生物学会年会  2022.12 

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    Event date: 2022.11 - 2022.12

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:幕張メッセ   Country:Japan  

  • カイコ-バキュロウイルス発現系を用いた豚繁殖・呼吸障害症候群ウイルスに対する高分子化ワクチンの開発

    #長谷川真理子, @李在萬, @増田亮津, @宮田健, @門宏明, @藤田龍介, @日下部宜宏

    第45回日本分子生物学会年会  2022.12 

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    Event date: 2022.11 - 2022.12

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:幕張メッセ   Country:Japan  

  • カイコ蛹からの流行型HuNoV-VLPsの精製検討

    #森本啓介, @増田亮津, @李在萬, @門宏明, @藤田龍介, @日下部宜宏

    第45回日本分子生物学会年会  2022.12 

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    Event date: 2022.11 - 2022.12

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:幕張メッセ   Country:Japan  

  • 巣をモデルとした配偶子形成に関与するカオナシ遺伝子の解析

    #柿野耕平、@門宏明、@佐藤昌直、@藤井告、@日野真人、@李在萬、@藤田龍介、@日下部宜宏

    日本蚕糸学会第92回大会  2022.3 

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    Event date: 2022.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:オンライン開催   Country:Japan  

    Other Link: https://sites.google.com/view/jsss-yamaguchi92/

  • カイコを利用する産業創生 Invited

    @李在萬

    日本蚕糸学会第93回大会  2022.3 

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    Event date: 2022.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:オンライン開催   Country:Japan  

    Other Link: https://sites.google.com/view/jsss-yamaguchi92/

  • 昆虫生産系におけるプロタンパク質転換システムの改変によるTGF-β成熟の効率化

    @小山茜音、#李在萬、@増田亮津、#門宏明、#藤田龍介、#日下部宜宏

    日本蚕糸学会第91回大会  2021.3 

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    Event date: 2021.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:オンライン開催   Country:Japan  

    Other Link: https://sites.google.com/view/jsss-tsukuba91

  • カイコの尿酸代謝を支配する抗酸化酵素の同定

    @藤井告, #田中未祐,#柿野耕平, @福森寿善, @金児雄, @李在萬, @日下部宜宏, @伴野豊

    日本蚕糸学会第91回大会  2021.3 

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    Event date: 2021.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:オンライン開催   Country:Japan  

    Other Link: https://sites.google.com/view/jsss-tsukuba91

  • カイコ-バキュロウイルス発現系を用いたSARS-CoV-2に対するCR3022抗体の生産

    @海老原健、#高橋大輔、#李在萬、@増田亮津、#藤田龍介、#門宏明、#植田正、#日下部宜宏

    日本蚕糸学会第91回大会  2021.3 

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    Event date: 2021.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:オンライン開催   Country:Japan  

    Other Link: https://sites.google.com/view/jsss-tsukuba91

  • カイコ-バキュロウイルス発現系を用いて生産したノロウイルス様粒子の安定性向上の検討

    @森本啓介、@増田亮津、#李在萬、@海老原健、#藤田龍介、#門宏明、#日下部宜宏

    日本蚕糸学会第91回大会  2021.3 

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    Event date: 2021.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:オンライン開催   Country:Japan  

    Other Link: https://sites.google.com/view/jsss-tsukuba91

  • カイコバキュロウイルス発現系を用いたヒトロタウイルス組換えワクチンの開発

    @長谷川真理子、#李在萬、@増田亮津、#藤田龍介、#日下部宜宏

    日本蚕糸学会第91回大会  2021.3 

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    Event date: 2021.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:オンライン開催   Country:Japan  

    Other Link: https://sites.google.com/view/jsss-tsukuba91

  • カイコ-バキュロウイルス発現系を用いた組換えヒト血管内皮細胞増殖因子の高効率生産

    #増田亮津,@唐崎紀子,@伴野 豊,@南畑孝介,@神谷典穂,@門宏明,@李在萬,@日下部宜宏

    第41回日本分子生物学会  2018.11 

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    Event date: 2018.11

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:神奈川   Country:Japan  

  • 昆虫工場におけるVLP生産

    @李在萬

    昆虫ポストゲノム研究会  2018.10 

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    Event date: 2018.10

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:八丈島   Country:Japan  

  • 昆虫工場を用いたウイルス様粒子ワクチンの生産系の確立とその応用

    #増田亮津,@宮田健,@南畑孝介,@神谷典穂,@李在萬,@日下部宜宏

    第71回日本衛生動物学会大会  2019.4 

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    Event date: 2018.4

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:山口大学   Country:Japan  

  • カイコ生産ウイルス様粒子表面への抗原ディスプレイの試み

    #増田亮津,@南畑孝介,@神谷典穂,@李在萬,@日下部宜宏

    平成31年蚕糸学会本会  2019.3 

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    Event date: 2018.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:東京農工大学   Country:Japan  

  • 部位特異的架橋技術を用いたカイコ生産ウイルス様粒子表面への分子ディスプレイ

    増田亮津、木下友里恵、森尾明大、宮田健、神谷典穂、李在萬、日下部宜宏

    平成30年蚕糸学会本会  2018.3 

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    Event date: 2018.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:名古屋大学   Country:Japan  

  • Characterization of Porcine Circovirus Type 2 Virus-like Particles produced by Silkworm-baculovirus Expression Vector System International conference

    Akitsu Masuda, Yurie Kinoshita, Akihiro Morio, Takeshi Miyata, Jae Man Lee, Takahiro Kusakabe

    International Symposium on Agricultural, Food, Environmental and Life Sciences in Asia 2017  2017.11 

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    Event date: 2017.11

    Language:English   Presentation type:Oral presentation (general)  

    Country:Japan  

  • High-yield Production of Biologically Active Human Basic Fibroblast Growth Factor Using Silkworm-baculovirus Expression Vector System International conference

    Akitsu Masuda, Yurie Kinoshita, Akihiro Morio, Noriko Karasaki, Tsuneyuki Tatsuke, Hiroaki Mon, Kosuke Minamihata, Noriho Kamiya, Jae Man Lee, Takahiro Kusakabe

    International Symposium on Basic and Applied Research on Sericulture and Insect Sciences  2017.3 

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    Event date: 2017.2 - 2017.3

    Language:English   Presentation type:Oral presentation (general)  

    Country:Thailand  

  • Lipidation, delipidation and degradation of Atg8 in Bombyx cells

    2016.3 

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    Event date: 2016.3

    Language:English   Presentation type:Oral presentation (general)  

    Country:Japan  

  • ヒト糖転移酵素の導入によるカイコ培養細胞のN型糖鎖修飾改変の試み

    諸熊大輔, 徐剣, 門 宏明, 李在萬, 日下部 宜宏

    平成27年蚕糸学会本会  2016.3 

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    Event date: 2016.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:京京都工芸繊維大学(京都市左京区)   Country:Japan  

  • カイコ分散型動原体を構成するタンパク質の解析

    門 宏明, 李在萬, 日下部宜宏

    日本分子生物学会本会  2015.12 

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    Event date: 2015.12

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:神戸ポートアイランド   Country:Japan  

  • RNA-Seq-based transcriptome analysis of p38, ERK and JNK MAPK gene suppressions in cultured Bombyx mori cells

    2015.12 

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    Event date: 2015.12

    Language:English   Presentation type:Symposium, workshop panel (public)  

    Country:Japan  

  • カイコバキュロウイルス発現系を用いたヒト糖転移酵素の大量生産

    諸熊大輔, 門 宏明, 李在萬, 伴野豊, 日下部宜宏

    日本分子生物学会本会  2015.12 

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    Event date: 2015.12

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:神戸ポートアイランド   Country:Japan  

  • カイコにおけるTORシグナル経路の解明

    平田一真, 佐藤昌直, 徐剣, 季明明, 祝力, 日野真人, 山下真実, 諸熊大輔, 門 宏明, 李在萬, 日下部 宜宏

    平成27年蚕糸学会本会  2015.9 

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    Event date: 2015.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:北海道大学大学院農学研究院(北海道札幌市)   Country:Japan  

  • カイコ培養細胞におけるBmNPV 136遺伝子のプロモーター活性

    山下真実, 徐剣, 季明明, 門 宏明, 李在萬, 日下部 宜宏

    平成27年蚕糸学会本会  2015.9 

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    Event date: 2015.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:北海道大学大学院農学研究院(北海道札幌市)   Country:Japan  

  • カイコ複製関連遺伝子の網羅的ノックダウ ン解析

    日野真人, 門 宏明, 徐剣, 祝力, 李在萬, 日下部 宜宏

    平成27年蚕糸学会本会  2015.9 

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    Event date: 2015.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:北海道大学大学院農学研究院(北海道札幌市)   Country:Japan  

  • Novel kinetochore protein complex from silkworm holocentric chromosomes International conference

    29th Annual Symposiumof the Protein Society  2015.7 

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    Event date: 2015.7

    Language:English   Presentation type:Oral presentation (general)  

    Country:Spain  

  • Characterization of silkworm replication related proteins for making an artificial chromosome International conference

    The 4th Asia-Pacific Congress of Sericulture and Insect Biotechnology  2015.4 

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    Event date: 2015.4

    Language:English   Presentation type:Oral presentation (general)  

    Country:Korea, Republic of  

  • Engineering of silkworm-baculovirus expression system for efficient production of G protein -coupled receptors International conference

    Jianping Chen, Jian Xu,, Li Zhu, JAE MAN LEE, Takahiro Kusakabe

    The 4th Asia-Pacific Congress of Sericulture and Insect Biotechnology  2015.4 

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    Event date: 2015.4

    Language:English   Presentation type:Oral presentation (general)  

    Country:Korea, Republic of  

  • GFP-p62 degradation is an excellent marker of autophagic flux in Bombyx International conference

    The 4th Asia-Pacific Congress of Sericulture and Insect Biotechnology  2015.4 

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    Event date: 2015.4

    Language:English   Presentation type:Oral presentation (general)  

    Country:Korea, Republic of  

  • Synthesis of Complex-Type N-Glycans on Recombinant Proteins by Modifying N-Glycosylation Pathway in Cultured Silkworm Cells International conference

    The 4th Asia-Pacific Congress of Sericulture and Insect Biotechnology  2015.4 

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    Event date: 2015.4

    Language:English   Presentation type:Oral presentation (general)  

    Country:Korea, Republic of  

  • Establishiment of New insect Cell Lines by Using Cell Fusion Method International conference

    The 4th Asia-Pacific Congress of Sericulture and Insect Biotechnology  2015.4 

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    Event date: 2015.4

    Language:English   Presentation type:Oral presentation (general)  

    Country:Korea, Republic of  

  • Characterization of BmNPV Promoters for Improving Exogenous Genes Expression in Bombyx Cells International conference

    The 4th Asia-Pacific Congress of Sericulture and Insect Biotechnology  2015.4 

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    Event date: 2015.4

    Language:English   Presentation type:Oral presentation (general)  

    Country:Korea, Republic of  

  • Expression and Purification of IL-1b related proteins Using Silkworm Baculovirus Protein Expression System International conference

    Masato Hino, JAE MAN LEE, Mami Yamashita, Kazuma Hirata, Daisuke Morokuma, Takahiro Kusakabe

    International Symposium on Basic and Applied Research on Sericulture and Insect Sciences  2015.4 

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    Event date: 2015.4

    Language:English   Presentation type:Oral presentation (general)  

    Country:Korea, Republic of  

  • Improvement of Caenorhabditis elegans SID-1-Dependent DNA Delivery System in Cultured Silkworm Cells International conference

    International Symposium on Basic and Applied Research on Sericulture and Insect Sciences  2015.4 

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    Event date: 2015.4

    Language:English   Presentation type:Symposium, workshop panel (public)  

    Country:Korea, Republic of  

  • Expression, purification, and characterization of streptavidin mutants using Baculovirus-mediated silkworm protein expression system International conference

    International Symposium on Basic and Applied Research on Sericulture and Insect Sciences  2015.4 

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    Event date: 2015.4

    Language:English   Presentation type:Oral presentation (general)  

    Country:Korea, Republic of  

  • Efficient Endo H expression by silkworm baculovirys expression system International conference

    International Symposium on Basic and Applied Research on Sericulture and Insect Sciences  2015.4 

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    Event date: 2015.4

    Language:English   Presentation type:Symposium, workshop panel (public)  

    Country:Korea, Republic of  

  • Expression and characterization of short-type human alpha 1 acid glycoprotein (α1AGP∆) for glycobiological research in baculovirus expression system International conference

    Daisuke Morokuma, 門 宏明, JAE MAN LEE, Takahiro Kusakabe, YUTAKA BANNO

    2015.4 

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    Event date: 2015.4

    Language:English   Presentation type:Symposium, workshop panel (public)  

    Venue:Kyungpook National University, Daegu   Country:Korea, Republic of  

  • Expression, purification, and characterization of human alpha 1 acid glycoprotein (α1AGP) for glycobiological research in baculovirus expression system International conference

    Daisuke Morokuma, 門 宏明, JAE MAN LEE, Takahiro Kusakabe, YUTAKA BANNO

    2015.4 

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    Event date: 2015.4

    Language:English   Presentation type:Oral presentation (general)  

    Venue:Kyungpook National University, Daegu   Country:Korea, Republic of  

  • Expression and Purification of Fibroblast Growth Factors 1, 7, and 10 from Silkworm-Baculovirus Expression Vector System International conference

    International Symposium on Basic and Applied Research on Sericulture and Insect Sciences  2015.4 

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    Event date: 2015.4

    Language:English   Presentation type:Oral presentation (general)  

    Country:Korea, Republic of  

  • DNA methyltransferase DNMT-1 from Bombyx mori International conference

    International Symposium on Basic and Applied Research on Sericulture and Insect Sciences  2015.4 

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    Event date: 2015.4

    Language:English   Presentation type:Symposium, workshop panel (public)  

    Country:Korea, Republic of  

  • ヒトα1-酸性糖タンパク質(α1AGP)は昆虫細胞における糖鎖生物学研究のモデルタンパク質となる

    諸熊 大輔, 日下部 宜宏, 李 在萬, 門 宏明, 伴野 豊

    平成26年第37回日本分子生物学会本会  2014.11 

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    Event date: 2014.11

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:パシフィコ横浜   Country:Japan  

  • Biochemical characterization of Bombyx mori DNA methyltransferase-1 (BmDNMT-1) and its roles on transcriptional regulation

    Takumi Mitsudome, Hiroaki Mon, Masanao Sato, Yoshitaka Suetsugu, Kimiko Yamamoto, Zhiqing Li, Jian Xu,, Li Zu, JAE MAN LEE, Takahiro Kusakabe, Atsushi Masuda

    2014.11 

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    Event date: 2014.11

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Country:Japan  

  • Function of silkworm XBP1 during an ER stress response

    Saki Imai, Jian Xu,, Hiroaki Mon, JAE MAN LEE, Takahiro Kusakabe

    2014.11 

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    Event date: 2014.11

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Country:Japan  

  • Role of silkworm RISC components in the basal defense against Bombyx mori nucleopolyhedrovirus (BmNPV)

    2014.3 

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    Event date: 2014.3

    Language:English   Presentation type:Oral presentation (general)  

    Country:Japan  

  • カイコ動原体を構成するタンパク質の解析

    門 宏明, LEE JAE MAN, 日下部 宜宏

    平成26年蚕糸学会本会  2014.3 

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    Event date: 2014.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:日本大学生物資源科学部(藤沢)   Country:Japan  

  • Soaking RNAi and its application on insect sciences Invited International conference

    2013.5 

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    Event date: 2013.5

    Language:English   Presentation type:Symposium, workshop panel (public)  

    Country:Korea, Republic of  

  • カ イコ分散型動原体ループドメイン形成への CTCF, CP190 の関与

    益田篤, 佐藤昌直, 末次克行, 山本公子, 門 宏明, LEE JAE MAN, 日下部 宜宏

    平成25年蚕糸学会本会  2013.3 

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    Event date: 2013.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:福岡   Country:Japan  

  • BmDnmt1, BmDnmt2, bMBDの機能阻害細胞におけるDNAのメチル化

    満留卓実, 門 宏明, 佐藤昌直, 末次克行, 山本公子, 李志清, 徐剣, 祝力, LEE JAE MAN, 日下部 宜宏

    平成25年蚕糸学会本会  2013.3 

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    Event date: 2013.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:福岡   Country:Japan  

  • カイコ BES を用いたヒト β-defensin (DEFB)-1, 2, 3 と LL37 の発現と抗菌活性の測定

    福島舞, 飯山 和弘, LEE JAE MAN, 山下隼, 門 宏明, 古江増隆, 辻学, 日下部 宜宏

    平成24年第35回日本分子生物学会本会  2012.12 

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    Event date: 2012.12

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:福岡   Country:Japan  

  • カイコ分散型動原体染色体におけるクロマチンループ形成とその役割

    益田篤, 佐藤昌直, 門 宏明, LEE JAE MAN, 日下部 宜宏

    平成24年第35回日本分子生物学会本会  2012.12 

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    Event date: 2012.12

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:福岡   Country:Japan  

  • カイコにおけるDNAメチル化因子の機能解析

    満留卓実, 門 宏明, 徐剣, LEE JAE MAN, 日下部 宜宏

    平成24年第35回日本分子生物学会本会  2012.12 

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    Event date: 2012.12

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:福岡   Country:Japan  

  • カイコにおけるヒストンH3S10リン酸化の役割

    吉村開人, 田附 常幸, 門 宏明, LEE JAE MAN, 日下部 宜宏

    平成24年第35回日本分子生物学会本会  2012.12 

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    Event date: 2012.12

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:福岡   Country:Japan  

  • カイコ培養細胞NHEJにおける修復関連遺伝子ノックダウンの影響

    藤井美江, 門 宏明, 高橋将晃, 光延仁志, LEE JAE MAN, 日下部 宜宏

    平成24年第35回日本分子生物学会本会  2012.12 

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    Event date: 2012.12

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:福岡   Country:Japan  

  • カイコ培養細胞による多分岐型N-グリカンを持つ糖タンパク質の生産

    永田祐大, 門 宏明, LEE JAE MAN, 日下部 宜宏

    平成24年第35回日本分子生物学会本会  2012.12 

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    Event date: 2012.12

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:福岡   Country:Japan  

  • Molecular cloning and characterization of asparagine synthetase in the silkworm, Bombyx mori

    2012.3 

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    Event date: 2012.3

    Presentation type:Oral presentation (general)  

    Country:Japan  

  • カイコ培養細胞における N-グリコシル化経路の改変とタンパク質発現系への応用

    永田祐大・副嶋泰彦・門 宏明・李 在萬・日下部宜宏

    平成24年蚕糸学会本会  2012.3 

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    Event date: 2012.3

    Presentation type:Oral presentation (general)  

    Venue:福岡   Country:Japan  

  • カイコ BES によるヒト DEFB1, 2, 3 と LL37 の発現と DEFB2 が菌の生存率に与える影響

    福島 舞・飯山和弘・李 在萬・山下 隼・古江増隆・辻 学・日下部宜宏

    平成24年蚕糸学会本会  2012.3 

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    Event date: 2012.3

    Presentation type:Oral presentation (general)  

    Venue:福岡   Country:Japan  

  • BES におけるタンパク質分泌量への小胞体シャペロンの関与

    工藤遼亮・日下部宜宏・李 在萬・門 宏明

    平成24年蚕糸学会本会  2012.3 

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    Event date: 2012.3

    Presentation type:Oral presentation (general)  

    Venue:福岡   Country:Japan  

  • カイコ培養細胞における糖鎖修飾部位改変組換え分泌タンパク質の生産

    副嶋泰彦・李 在萬・日下部宜宏

    平成24年蚕糸学会本会  2012.3 

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    Event date: 2012.3

    Presentation type:Oral presentation (general)  

    Venue:福岡   Country:Japan  

  • Positional cloning and functional analysis of candidate genes responsible for AcNPV sensitivity in silkworm, Bombyx mori

    2012.3 

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    Event date: 2012.3

    Presentation type:Oral presentation (general)  

    Country:Japan  

  • カイコにおける DNA 二重鎖切断関連因子の解析

    藤井美江・門 宏明・高橋将晃・光延 仁・李 在萬・日下部宜宏

    平成24年蚕糸学会本会  2012.3 

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    Event date: 2012.3

    Presentation type:Oral presentation (general)  

    Venue:福岡   Country:Japan  

  • 点変異導入によるカイコヒストン H3-K4, K9, K27, S10 修飾の機能解析

    吉村開人・田附常幸・門 宏明・李 志清・李 在萬・日下部宜宏

    平成24年蚕糸学会本会  2012.3 

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    Event date: 2012.3

    Presentation type:Oral presentation (general)  

    Venue:福岡   Country:Japan  

  • BmTudor-sn interact with BmAgo1 and BmAgo2 not to be involved in RNAi pathway, while to participate in stress granule formation in BmN4 cells

    2012.3 

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    Event date: 2012.3

    Presentation type:Oral presentation (general)  

    Country:Japan  

  • カイコにおける RNAi 経路の解析

    吉末真吾・田附常幸・阪下浩介・門 宏明・李 在萬・日下部宜宏

    平成24年蚕糸学会本会  2012.3 

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    Event date: 2012.3

    Presentation type:Oral presentation (general)  

    Venue:福岡   Country:Japan  

  • カイコにおける分散型動原体タンパク質の解析/Analysis of the kinetochore proteins in holocentric chromosomes of Bombyx mori

    門宏明・李在萬・日下部宜宏

    平成23年第34回日本分子生物学会本会  2011.12 

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    Event date: 2011.12

    Presentation type:Symposium, workshop panel (public)  

    Venue:神戸   Country:Japan  

  • カイコ培養細胞におけるDNA二重鎖切断修復経路の解析/Analysis of DNA double-strand break repair pathways in silkworm cells

    藤井美江、門宏明、高橋将晃、光延仁志、李在萬、日下部宜宏

    平成23年第34回日本分子生物学会本会  2011.12 

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    Event date: 2011.12

    Presentation type:Symposium, workshop panel (public)  

    Venue:神戸   Country:Japan  

  • カイコ BES を用いたヒト抗菌タンパク質の生産と活性評価/Production of human antimicrobial peptides using baculovirus expression system and evaluation of its activity

    福島舞・飯山和弘・李在萬・山下隼・古江増隆・辻学・日下部宜宏

    平成23年第34回日本分子生物学会本会  2011.12 

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    Event date: 2011.12

    Presentation type:Symposium, workshop panel (public)  

    Venue:神戸   Country:Japan  

  • カイコ β-N-acetylglucosaminidase (BmFDL) のノックダウンが N- グリカン形成に与える影響/Effect of RNAi for BmFDL in the N-glycosylation pathway

    永田祐大・副嶋泰彦・門宏明・李在萬・日下部宜宏

    平成23年第34回日本分子生物学会本会  2011.12 

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    Event date: 2011.12

    Presentation type:Symposium, workshop panel (public)  

    Venue:神戸   Country:Japan  

  • バキュロウイルス発現系における小胞体シャペロンの関与/Roles of the endoplasmic reticulam chaperones in recombinant protein secretion in BES

    工藤遼亮・門宏明・李在萬・日下部宜宏

    平成23年第34回日本分子生物学会本会  2011.12 

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    Event date: 2011.12

    Presentation type:Symposium, workshop panel (public)  

    Venue:神戸   Country:Japan  

  • Analysis of P-bodies and stress granules in silkworm BmN4 cells

    2011.12 

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    Event date: 2011.12

    Presentation type:Symposium, workshop panel (public)  

    Country:Japan  

  • カイコにおけるテトラサイクリン誘導型遺伝子発現システムの改良/Improvement of tetracycline-inducible gene expression systems in the silkworm, Bombyx mori

    田附常幸・藤井美江・永田祐大・福島舞・李在萬・日下部宜宏

    平成23年第34回日本分子生物学会本会  2011.12 

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    Event date: 2011.12

    Presentation type:Symposium, workshop panel (public)  

    Venue:神戸   Country:Japan  

  • カイコ動原体タンパク質の解析

    門宏明・李在萬・日下部宜宏

    平成23年蚕糸学会本会  2011.3 

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    Event date: 2011.3

    Presentation type:Oral presentation (general)  

    Venue:東京   Country:Japan  

  • 糖鎖構造の改変によるカイコーバキュロウイルス発現系への応用

    永田祐大・副嶋泰彦・門宏明・李在萬・日下部宜宏

    平成23年蚕糸学会本会  2011.3 

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    Event date: 2011.3

    Presentation type:Oral presentation (general)  

    Venue:東京   Country:Japan  

  • カイコ熱ショックファクターBmHsf1, BmHsf1lpの転写活性化機構に関する研究

    山下隼・福島舞・李在萬・日下部宜宏

    平成23年蚕糸学会本会  2011.3 

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    Event date: 2011.3

    Presentation type:Oral presentation (general)  

    Venue:東京   Country:Japan  

  • カイコにおけるフアンコニ経路因子FancMの機能解析

    菅原亮平・門宏明・李在萬・日下部宜宏

    平成23年蚕糸学会本会  2011.3 

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    Event date: 2011.3

    Presentation type:Oral presentation (general)  

    Venue:東京   Country:Japan  

  • Molecular characterization of the Fanconi anemia protein FancM in silkworm, Bombyx mori

    Ryohei Sugahara, Hiroaki Mon, Jae Man Lee, and Takahiro Kusakabe

    2010.12 

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    Event date: 2010.12

    Presentation type:Symposium, workshop panel (public)  

    Country:Japan  

    Molecular characterization of the Fanconi anemia protein FancM in silkworm, Bombyx mori

  • カイコ Dicer-2 のクローニングと機能解析

    吉末 真吾,田附 常幸,正木 夕輝,阪下 浩介,李 在萬,日下部 宜宏

    平成22年日本分子生物学会本会  2010.12 

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    Event date: 2010.12

    Presentation type:Symposium, workshop panel (public)  

    Venue:神戸   Country:Japan  

  • BESにおけるタンパク質分泌量への小胞体シャペロンの関与

    Ryosuke Kudo,Jun Yamashita,Hiroaki Mon,Jae Man Lee and Takahiro Kusakabe

    平成22年日本分子生物学会本会  2010.12 

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    Event date: 2010.12

    Presentation type:Symposium, workshop panel (public)  

    Venue:神戸   Country:Japan  

  • Silkworm Argonaute2 homolog is involved in exso-siRNA and endo-siRNA pathway

    Yuki Masaki, Tsuneyuki Tatsuke, Kosuke Sakashita, Shingo Yoshizue, JaeMan Lee, and Takahiro Kusakabe

    2010.12 

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    Event date: 2010.12

    Presentation type:Symposium, workshop panel (public)  

    Country:Japan  

    Silkworm Argonaute2 homolog is involved in exso-siRNA and endo-siRNA pathway

  • Identification and Expression Pattern Analysis of Polycomb Group Genes in the Silkworm, Bombyx mori,

    Zhiqing Li, Tsuneyuki Tatsuke, Hiroaki Mon, JaeMan Lee, and Takahiro Kusakabe

    2010.12 

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    Event date: 2010.12

    Presentation type:Symposium, workshop panel (public)  

    Country:Japan  

    Identification and Expression Pattern Analysis of Polycomb Group Genes in the Silkworm, Bombyx mori,

  • カイコ Piwi タンパク質による転写抑制効果の解析

    田附常幸、正木夕輝、吉末真吾、光延仁志、阪下浩介、李在萬、日下部宜宏

    平成22年日本分子生物学会本会  2010.12 

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    Event date: 2010.12

    Presentation type:Symposium, workshop panel (public)  

    Venue:神戸   Country:Japan  

  • Efficient Protein Expression in Bombyx mori Larvae of the Strains Highly Sensitive to B. mori Nucleopolyhedrovirus Invited International conference

    Takahiro Kusakabe, JaeMan Lee

    The bioprocessing summit 2010: Baculovirus Technology  2010.8 

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    Event date: 2010.8

    Presentation type:Oral presentation (general)  

    Country:United States  

  • カイコにおけるDNA損傷修復機構ファンコニ経路の解析

    管原亮平・門宏明・李在萬・河口豊・日下部宜宏,

    平成22年蚕糸学会本会  2010.4 

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    Event date: 2010.4

    Presentation type:Oral presentation (general)  

    Venue:信州   Country:Japan  

  • カイコ培養細胞 BmN4 における Piwi サブファミリータンパク質の転写抑制への関与

    田附常幸・光延仁志・正木夕輝・阪下浩介・吉末真吾・李在萬・河口豊・日下部宜宏

    平成22年蚕糸学会本会  2010.4 

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    Event date: 2010.4

    Presentation type:Oral presentation (general)  

    Venue:信州   Country:Japan  

  • Functional analysis of BmHsp90 cochaperones for hormone receptors in the silkworm.

    Jun Yamashita, Hiroaki Mon, Hitoshi Mitsunobu, Shun Sawatsubashi, Shigeaki Kato, Jae Man Lee, Yutaka Kawaguchi, Takahiro Kusakabe

    2009.12 

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    Event date: 2009.12

    Presentation type:Oral presentation (general)  

    Country:Japan  

  • Characterization of Light Chain Homologue Ferritin and the Efficient Production on Silkworm Expressing Cytoplasmic Chaperones

    SunMee Hong, Hitoshi Mitsunobu, Jun Yamashita, Hideki Sezutsu, Toshiki Tamura, Hiroaki Mon, JaeMan Lee , Yutaka Kawaguchi , Takahiro Kusakabe

    2009.12 

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    Event date: 2009.12

    Presentation type:Oral presentation (general)  

    Country:Japan  

  • The analysis of mechanism of the factors involved in RNAi in silkworm

    Yuki Masaki, Tsuneyuki Tatsuke, Kosuke Sakashita, Shingo Yoshizue, JaeMan Lee, Yutaka Kawaguchi, Takahiro Kusakabe

    2009.12 

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    Event date: 2009.12

    Presentation type:Oral presentation (general)  

    Country:Japan  

  • Interaction between Tip60 and Histones in Bombyx mori

    Kiyoko Miyata, Hitoshi Mitsunobu, Hiroaki Mon, JaeMan Lee, Yutaka Kawaguchi, Takahiro Kusakabe

    2009.12 

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    Event date: 2009.12

    Presentation type:Oral presentation (general)  

    Country:Japan  

  • Cloning and characterization of p53 homolog in silkworm, Bombyx mori

    Kokoro Shimada, Masateru Takahashi, Yoshiyuki Miyagawa, Kazuei Mita, Tsuneyuki Tatsuke, JaeMan Lee, Takahiro Kusakabe, Yutaka Kawaguchi

    2009.12 

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    Event date: 2009.12

    Presentation type:Oral presentation (general)  

    Country:Japan  

  • Analysis of DNA interstrand crosslink repair in silkworm, Bombyx mori

    Shinji Ohkubo, Ryohei Sugahara, Hiroaki Mon, Masateru Takahashi, Tsuneyuki Tatsuke, Yosuke Taniguchi, Shigeki Sasaki, Jaeman Lee, Yutaka Kawaguchi and Takahiro Kusakabe

    2009.12 

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    Event date: 2009.12

    Presentation type:Oral presentation (general)  

    Country:Japan  

  • Analysis of Post-translational modifications of histones in Bombyx mori

    Makiko Izumi , Hitoshi Mitsunobu , Hiroaki Mon , Kazuhiro Iiyama , Hiroyuki Jikuya, JaeMan Lee , Yutaka Kawaguchi , Takahiro Kusakabe

    2009.12 

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    Event date: 2009.12

    Presentation type:Oral presentation (general)  

    Country:Japan  

  • Analysis of the interactions between BRCA2 and RAD51 in silkworm, Bombyx mori

    Hiroaki Mon, Ryohei Sugahara, Jaeman Lee, Yutaka Kawaguchi and Takahiro Kusakabe

    2009.12 

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    Event date: 2009.12

    Presentation type:Oral presentation (general)  

    Country:Japan  

  • Analysis of the interactions between BRCA2 and RAD51 in silkworm, Bombyx mori

    Hiroaki Mon, Ryohei Sugahara, Jaeman Lee, Yutaka Kawaguchi and Takahiro Kusakabe

    2009.12 

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    Event date: 2009.12

    Presentation type:Oral presentation (general)  

    Country:Japan  

  • カイコにおける相同組換え関連遺伝子の機能解析

    門 宏明・李 在萬・日下部宜宏・河口 豊

    昆虫ワークショップ2009  2009.9 

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    Event date: 2009.9

    Presentation type:Oral presentation (general)  

    Country:Japan  

  • カイコにおけるDNA鎖間架橋修復の解析

    大久保慎司・管原亮平・門 宏明・高橋将晃・李 在萬・日下部宜宏・河口 豊

    昆虫ワークショップ2009  2009.9 

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    Event date: 2009.9

    Presentation type:Oral presentation (general)  

    Country:Japan  

  • The interaction of BmHsp90 cochaperones with mammalian MAPKs and insect hormone receptors.

    2009.3 

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    Event date: 2009.3

    Presentation type:Oral presentation (general)  

    Country:Japan  

  • BmNPV高感受性カイコを用いた交配系統による効果的タンパク発現の試み(2)

    李 在萬・久保雄二・大畑 敬一・伴野 豊・河口 豊・日下部 宜宏

    蚕糸学会本会(東京)  2009.3 

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    Event date: 2009.3

    Presentation type:Oral presentation (general)  

    Venue:名古屋大学   Country:Japan  

  • カイコにおけるファンコニ経路因子FancI, D2複合体の解析

    管原亮平・門宏明・大久保慎司・谷口陽祐・佐々木茂貴・李在萬・河口豊・日下部宜宏

    蚕糸学会本会(東京)  2009.3 

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    Event date: 2009.3

    Presentation type:Oral presentation (general)  

    Venue:名古屋大学   Country:Japan  

  • カイコにおけるヘテロクロマチン誘導による転写抑制

    光延仁志・竹野奈津美・門 宏明・李 在萬・小林 功・田村俊樹・小倉絵里・蒲池雄介・日下部宜宏・河口 豊

    蚕糸学会本会(東京)  2009.3 

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    Event date: 2009.3

    Presentation type:Oral presentation (general)  

    Country:Japan  

  • 高効率的組換えタンパク質発現用宿主としてのカイコ系統の開発(3)

    李 在萬・張平波・伴野 豊・河口 豊・日下部 宜宏

    蚕糸学会本会(名古屋)  2008.3 

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    Presentation type:Oral presentation (general)  

    Venue:名古屋大学   Country:Japan  

  • カイコのXDH遺伝子へのFLAGタグ配列のノックイン

    @藤井告, #柿野耕平, @李在萬, @日下部宜宏, @伴野豊

    日本蚕糸学会第93回大会  2022.3 

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    Event date: 2022.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:オンライン開催   Country:Japan  

    Other Link: https://sites.google.com/view/jsss-yamaguchi92/

  • カイコ培養細胞における相同組換え修復測定系の確立とその解析

    門 宏明、日下部宜宏、青木智佐、李 在 萬、河口 豊、古賀克己

    日本蚕糸学会第72回学術講演会  2002.4 

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    Venue:福岡   Country:Japan  

  • カイコの熱ショック蛋白質HSC70-4のプロモーター領域を含む遺伝子の構造と発現解析

    李 在 萬、日下部宜宏、河口豊、古賀克己

    日本蚕糸学会第72回学術講演会  2002.4 

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    Venue:福岡   Country:Japan  

  • Molecular characterization of the promoter and structure of complete sequence of the gene coding for the silkworm translationally controlled tumor protein(P23/TCTP) International conference

    Lee, J.M., Kusakabe, T., Kawaguchi, Y., Nho, S.K. and Koga K.

    The 2002 Joint Meeting of the Korean Society of Sericultural Science and the Japanese Society of Sericultural Science  2002.10 

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    Country:Korea, Republic of  

  • カイコ培養細胞における相同組換え修復の解析

    門 宏明、日下部宜宏、青木智佐、李 在 萬、河口 豊、古賀克己

    第25回日本分子生物学会年会  2002.12 

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    Venue:横浜   Country:Japan  

  • Molecular characterization of the translationally controlled tumor protein (TCTP/P23) promoter and complete sequence from the silkworm, Bombyx mori

    Lee, J.M., Kusakabe, T., Kawaguchi, Y. and Koga K.

    2002.12 

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    Country:Japan  

  • カイコ培養細胞におけるDNA二重鎖切断修復経路の解析

    門 宏明、日下部宜宏、青木智佐、李 在 萬、河口 豊、古賀克己

    日本蚕糸学会第73回学術講演会  2003.3 

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    Venue:東京   Country:Japan  

  • 遺伝的組換えに欠損がみられる突然変異系統r20を用いた減数分裂期関連因子の解析

    宮川世志幸、日下部宜宏、李 在 萬、河口 豊、古賀克己

    日本蚕糸学会第59回九州支部・第69回関西支部合同大会  2003.10 

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    Venue:京都   Country:Japan  

  • Construction of constitutive gene expression systems using the HSC70-4 and TCTP promoters International conference

    Lee, J.M., Kusakabe, T., Kawaguchi, Y., Nho, S.K. and Koga K.

    The 2002 Joint Meeting of the Korean Society of Sericultural Science and the Japanese Society of Sericultural Science.  2003.11 

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    Country:Japan  

  • φC31 integraseによるカイコ染色体上での部位特異的遺伝子導入

    中山 岳、李 在 萬、日下部宜宏、河口 豊、古賀克己、神田俊男、田村俊樹

    第26回日本分子生物学会年会  2003.12 

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    Venue:神戸   Country:Japan  

  • バキュロウイルス形質転換法を利用したRNAi誘導ベクターの構築

    荒神佳人、李 在 萬、日下部宜宏、古賀克己、河口 豊

    日本蚕糸学会第60回九州支部・第70回関西支部合同大会  2004.11 

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    Venue:福岡   Country:Japan  

  • φC31 インテグラ−ゼを用いた部位特異的遺伝子導入によるトランスジェニックカイコの作出

    中山 岳、李 在 萬、日下部宜宏、河口 豊、古賀克己、神田俊男、田村俊樹

    日本蚕糸学会第60回九州支部・第70回関西支部合同大会  2004.11 

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    Venue:福岡   Country:Japan  

  • 新規精巣成熟関連因子BmAha1タンパク質は精子細胞に蓄積する

    宮川世志幸、日下部宜宏、李 在 萬、古賀克己、河口 豊

    日本蚕糸学会第60回九州支部・第70回関西支部合同大会  2004.11 

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    Venue:福岡   Country:Japan  

  • アリジゴク共生細菌の産生する殺虫活性因子に関する研究

    江上郁夫、飯山和弘、千枝結香、猪野直美、長谷川啓一、李 在 萬、日下部宜宏、青木智佐、清水 進

    日本蚕糸学会第60回九州支部・第70回関西支部合同大会  2004.11 

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    Venue:福岡   Country:Japan  

  • Pseudomonas aeruginosa gacA 変異株のカイコに対する病原性(3)

    千枝結香、飯山和弘、青木智佐、李 在 萬、日下部宜宏、清水 進

    日本蚕糸学会第60回九州支部・第70回関西支部合同大会  2004.11 

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    Venue:福岡   Country:Japan  

  • バキュロウイルス形質転換法を利用したRNAiによるノックダウンベクターの開発

    荒神佳人、李 在 萬、日下部宜宏、河口 豊

    第27回日本分子生物学会年会  2004.12 

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    Venue:神戸   Country:Japan  

  • φC31 integraseによるカイコ染色体上での部位特異的遺伝子導入

    中山 岳、李 在 萬、日下部宜宏、河口 豊、古賀克己、神田俊男、田村俊樹

    第27回日本分子生物学会年会  2004.12 

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    Venue:神戸   Country:Japan  

  • カイコ精巣におけるAha1タンパク質の発現局在及び機能解析

    宮川世志幸、李 在 萬、古賀克己、河口 豊、日下部宜宏

    第27回日本分子生物学会年会  2004.12 

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    Venue:神戸   Country:Japan  

  • カイコにおける構成的遺伝子発現系の構築と解析

    李 在萬、日下部 宜宏、河口 豊

    日本蚕糸学会第75回学術講演会  2005.4 

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    Venue:東京   Country:Japan  

  • DNA repair and recombination in insect cells International conference

    Takahiro Kusakabe, Hiroaki Mon, Haruna Tsukioka, Lee JaeMan, Yutaka Kawaguchi

    Hyderabard; India  2006.1 

  • カイコにおける核内転写長鎖dsRNAによるRNAiについて

    荒神佳人・李 在萬・日下部宜宏・河口 豊

    蚕糸学会本会(京都)  2006.1 

  • 高効率組換えタンパク質発現用宿主としてのカイコ系統の開発

    李 在萬・川上直哉・日下部宜宏・河口 豊

    蚕糸学会本会(京都)  2006.1 

  • カイコにおける相同組換えに関わる遺伝子の機能解析(2)

    門 宏明・管原 亮平・李 在萬・日下部宜宏・河口 豊

    蚕糸学会本会(京都)  2006.1 

  • カイコHP1α、βのクローニングと機能解析

    光延仁志・門 宏明・高橋将晃・李 在萬・日下部宜宏・河口 豊

    蚕糸学会本会(京都)  2006.1 

  • Double-strand break repair by homologous recombination in silkworm cells. International conference

    Hiroaki Mon, Ryohei Sugahara, JaeMan Lee, Yutaka Kawaguchi, Takahiro Kusakabe

    20th IUBMB International Congress of Biochemistry and Molecular Biology  2006.1 

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    Country:Japan  

  • Molecular cloning and characterization of HP1a, HP1b in the silkworm, Bombyx mori. International conference

    Hitoshi Mitsunobu, Hiroaki Mon, Masateru Takahashi, Jae Man Lee, Yutaka Kawaguchi, Takahiro Kusakabe

    20th IUBMB International Congress of Biochemistry and Molecular Biology  2006.1 

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    Country:Japan  

  • A proteomic approach to silkworm proteins responding to g -irradiation. International conference

    Hiroko Yoshida, Masateru Takahashi, Pingbo Zhang, Yoshinori Ueno, Hiroaki Mon, Jae Man Lee, Yutaka Kawaguchi, Takahiro Kusakabe

    20th IUBMB International Congress of Biochemistry and Molecular Biology  2006.1 

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    Country:Japan  

  • Molecular cloning and characterization of FANCD2 in Bombyx mori International conference

    Ryohei Sugahara, Hiroaki Mon, Jae Man Lee, Takahiro Kusakabe, Yutaka Kawaguchi

    20th IUBMB International Congress of Biochemistry and Molecular Biology  2006.1 

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    Country:Japan  

  • Molecular cloning and characterization of HSP90 cochaperones in the silkworm, Bombyx mori International conference

    Jun Yamashita, Yoshitaka Miyagawa, Hiroaki Mon, Hitoshi Mistunobu, Jae Man Lee, Yutaka Kawaguchi, Takahiro Kusakabe

    20th IUBMB International Congress of Biochemistry and Molecular Biology  2006.1 

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    Country:Japan  

  • Cellular response to DNA double strand beraks (DSBs) in cultured silkworm cells International conference

    Takahiro Kusakabe, Hiroaki Mon, Masateru Takahashi, JaeMan Lee, Yutaka Kawaguchi

    The Workshop on Lepidopteran Molecular Biology and Genetics  2006.1 

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    Country:Greece  

  • Double-strand break repair by homologous recombination in silkworm cells International conference

    Hiroaki Mon, Ryohei Sugahara, JaeMan Lee, Yutaka Kawaguchi, Takahiro Kusakabe

    The Workshop on Lepidopteran Molecular Biology and Genetics  2006.1 

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    Country:Greece  

  • A Large Amount of dsRNA affects on Gene Expression in Cultured Silkworm Cells International conference

    Kousuke Sakashita, JaeMan Lee, Yutaka Kawaguchi, Takahiro Kusakabe

    Asia-Pacific Congress of Sericulture & Insect Biotechnology  2006.1 

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    Country:Korea, Republic of  

  • Proteomic Analysis of Silkworm Proteins Responding to DNA Damage Induced by Ionizing Radiation International conference

    Hiroko Yoshida, Masateru Takahashi, Hiroaki Mon, Pingbo Zhang, Yoshinori Ueno, Jae Man Lee, Yutaka Kawaguchi, Takahiro Kusakabe

    Asia-Pacific Congress of Sericulture & Insect Biotechnology  2006.1 

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    Country:Korea, Republic of  

  • Molecular Cloning and Functional Characterization of the Fanconi Anemia Protein, FANCD2 in Bombyx mori International conference

    Ryohei Sugahara, Hiroaki Mon, Jae Man Lee, Yutaka Kawaguchi, Takahiro Kusakabe

    Asia-Pacific Congress of Sericulture & Insect Biotechnology  2006.1 

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    Country:Korea, Republic of  

  • Molecular Cloning and Characterization of Hsp70, Hsc70-4, and Hsp40 in the silkworm, Bombyx mori International conference

    Jun Yamashita, Yoshitaka Miyagawa, Hitoshi Mitsunobu, Hiroaki Mon, Jae Man Lee, Yutaka Kawaguchi, Takahiro Kusakabe

    Asia-Pacific Congress of Sericulture & Insect Biotechnology  2006.1 

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    Country:Korea, Republic of  

  • Bombyx mori as a model host for Pseudomonas aeruginosa infection International conference

    Kazuhiro Iiyama, Yuuka Chieda, Jaeman Lee, Takahiro kusakabe, Chisa Yasunaga-Aoki, Susumu Shimizu

    Asia-Pacific Congress of Sericulture & Insect Biotechnology  2006.1 

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    Country:Korea, Republic of  

  • uvrC is necessary for Pseudomonas aeruginosa PAO1 to be highly virulent in Bombyx mori International conference

    Yuuka Chieda, Kazuhiro Iiyama, Jaeman Lee, Takahiro kusakabe, Chisa Yasunaga-Aoki, Susumu Shimizu

    Asia-Pacific Congress of Sericulture & Insect Biotechnology  2006.10 

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    Country:Korea, Republic of  

  • Molecular cloning and functional characterization of the Fanconi anemia protein, FANCD2 in Bombyx mori International conference

    Ryohei Sugahara, Hiroaki Mon, Jae Man Lee, Yutaka Kawaguchi

    Asia-Pacific Congress of Sericulture & Insect Biotechnology  2006.10 

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    Country:Korea, Republic of  

  • Molecular cloning and characterization of Hsp70, Hsc70-4, and Hsp40 in the silkworm, Bombyx mori International conference

    Jun Yamashita, Yoshitaka Miyagawa, Hitoshi Mitsunobu, Hiroaki Mon, Jae Man Lee, Yutaka Kawaguchi, Takahiro Kusakabe

    Asia-Pacific Congress of Sericulture & Insect Biotechnology  2006.10 

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    Country:Korea, Republic of  

  • Proteomic analysis of silkworm proteins responding to DNA damage induced by ionizing radiation International conference

    Hiroko Yoshida, Msateru Takahashi, Hiroaki Mon, Pingbo Zhang, Yoshinori Ueno, Jae Man Lee, Yutaka Kawaguchi, Takahiro Kusakabe

    Asia-Pacific Congress of Sericulture & Insect Biotechnology  2006.10 

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    Country:Korea, Republic of  

  • A large amount of dsRNA affects on gene expression in cultured silkworm cells International conference

    Kosuke Sakashita, Jae Man Lee, Yutaka Kawaguchi, Takahiro Kusakabe

    Asia-Pacific Congress of Sericulture & Insect Biotechnology  2006.10 

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    Country:Korea, Republic of  

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Industrial property rights

Patent   Number of applications: 4   Number of registrations: 1
Utility model   Number of applications: 0   Number of registrations: 0
Design   Number of applications: 0   Number of registrations: 0
Trademark   Number of applications: 0   Number of registrations: 0

Professional Memberships

  • 日本蚕糸学会九州支部会

  • 日本蚕糸学会

Committee Memberships

  • Councilor   Domestic

    2024.4 - 2026.3   

  • 日本蚕糸学会   会計   Domestic

    2024.4 - 2026.3   

  • Councilor   Domestic

    2022.4 - 2024.3   

  • 日本蚕糸学会   会計   Domestic

    2022.4 - 2024.3   

  • Councilor   Domestic

    2021.4 - 2022.3   

  • 日本蚕糸学会   会計   Domestic

    2021.4 - 2022.3   

  • Steering committee member   Domestic

    2019.4 - 2021.3   

  • 日本蚕糸学九州支部学会   会計   Domestic

    2019.4 - 2021.3   

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Academic Activities

  • Screening of academic papers

    Role(s): Peer review

    2022

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    Type:Peer review 

    Number of peer-reviewed articles in foreign language journals:2

  • Screening of academic papers

    Role(s): Peer review

    2021

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    Type:Peer review 

    Number of peer-reviewed articles in foreign language journals:2

  • Screening of academic papers

    Role(s): Peer review

    2020

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    Type:Peer review 

    Number of peer-reviewed articles in foreign language journals:2

  • Screening of academic papers

    Role(s): Peer review

    2019

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    Type:Peer review 

    Number of peer-reviewed articles in foreign language journals:2

  • Screening of academic papers

    Role(s): Peer review

    2018

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    Type:Peer review 

    Number of peer-reviewed articles in foreign language journals:4

  • 座長(Chairmanship)

    日本蚕糸学会九州支部会  ( 福岡 ) 2008.11 - 2016.5

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    Type:Competition, symposium, etc. 

  • 座長(Chairmanship)

    日本蚕糸学会九州支部会  ( 京都 ) 2007.11 - Present

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    Type:Competition, symposium, etc. 

  • 座長(Chairmanship)

    日本蚕糸学会九州支部会  ( 福岡 ) 2005.11 - Present

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    Type:Competition, symposium, etc. 

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Research Projects

  • カイコ体液プロテオームの遺伝的改変と有用タンパク質生産への利用

    Grant number:23H02223  2023 - 2026

    日本学術振興会  科学研究費助成事業  基盤研究(B)

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    Authorship:Coinvestigator(s)  Grant type:Scientific research funding

  • 「新型コロナワクチン・治療  発寄付口座」/COVID-19 症化治療を指向したグリーンファーマ研究の推進

    2020

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    Grant type:Donation

  • 新規に同定された短い遺伝子にコードされるホルモン様ペプチドの機能

    Grant number:18H02420  2018 - 2022

    日本学術振興会  科学研究費助成事業  基盤研究(B)

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    Authorship:Coinvestigator(s)  Grant type:Scientific research funding

  • 九大カイコバイオリソースを用いた人工細胞接着因子融合タンパク質の生産

    2017

    大学発ベンチャー事業育成支援プログラム

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    Authorship:Coinvestigator(s)  Grant type:On-campus funds, funds, etc.

  • 高機能ワクチンとしてのVLP修飾技術基盤の開発

    2016 - 2017

    QRプログラム(わかばチャレンジ)

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    Authorship:Principal investigator  Grant type:On-campus funds, funds, etc.

  • 昆虫工場におけるタンパク質糖鎖修飾の解析と改変

    Grant number:15H04612  2015 - 2018

    日本学術振興会  科学研究費助成事業  基盤研究(B)

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    Authorship:Principal investigator  Grant type:Scientific research funding

  • 昆虫細胞における新規遺伝子導入技術の確立

    Grant number:26660271  2014 - 2015

    科学研究費助成事業  萌芽研究

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    Authorship:Principal investigator  Grant type:Scientific research funding

  • 昆虫細胞におけるdsRNA応答機構の解析

    Grant number:23580077  2011 - 2013

    日本学術振興会  科学研究費助成事業  基盤研究(C)

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    Authorship:Principal investigator  Grant type:Scientific research funding

  • 昆虫分散型動原体染色体の謎

    2009 - 2010

    科学研究費助成事業  萌芽研究

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    Authorship:Coinvestigator(s)  Grant type:Scientific research funding

  • 高効率で安全な組換えウイルス増殖用カイコ細胞Bme21の無血清培養技術の開発

    2008

    独立行政法人 科学技術振興機構(JST)

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    Authorship:Principal investigator  Grant type:Contract research

  • 高効率物質生産系宿主としてのカイコのポストゲノム育種

    2006 - 2010

    新技術・新分野創出のための基礎研究推進事業

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    Authorship:Coinvestigator(s)  Grant type:Contract research

  • 病原細菌のDNA修復機構と宿主生体防御機構との相互作用

    2006

    九州大学農学研究院若手教員支援事業

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    Authorship:Coinvestigator(s)  Grant type:On-campus funds, funds, etc.

  • 昆虫における遺伝子ターゲティングの分子機構とその効率的利用

    Grant number:17380037  2005 - 2007

    日本学術振興会  科学研究費助成事業  基盤研究(B)

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    Authorship:Coinvestigator(s)  Grant type:Scientific research funding

  • 温泉ユスリカ由来耐熱タンパク質の解析

    Grant number:17658028  2005 - 2006

    科学研究費助成事業  萌芽研究

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    Authorship:Coinvestigator(s)  Grant type:Scientific research funding

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Class subject

  • 応用昆虫ウイルス学特論

    2023.12 - 2024.2   Winter quarter

  • 応用昆虫ウイルス学特論

    2023.12 - 2024.2   Winter quarter

  • 自然科学総合実験

    2023.10 - 2023.12   Fall quarter

  • 遺伝子組換え生物の利用と制御

    2023.4 - 2023.9   First semester

  • 遺伝子組換え生物の利用と制御

    2023.4 - 2023.9   First semester

  • 応用昆虫ウイルス学特論

    2022.12 - 2023.2   Winter quarter

  • 農業生物科学輪講

    2022.4 - 2023.3   Full year

  • 農業生物科学輪講

    2021.4 - 2022.3   Full year

  • 課題協学科目

    2020.10 - 2021.3   Second semester

  • 課題協学科目

    2020.10 - 2021.3   Second semester

  • 農業生物科学輪講

    2020.4 - 2021.3   Full year

  • 応用昆虫ウイルス学特論

    2019.12 - 2020.2   Winter quarter

  • 農業生物科学演習第二

    2019.10 - 2020.3   Second semester

  • 農業生物科学演習第一

    2019.10 - 2020.3   Second semester

  • 農業生物科学プロジェクト演習

    2019.4 - 2020.3   Full year

  • 農業生物科学輪講

    2019.4 - 2020.3   Full year

  • ティーチング演習

    2019.4 - 2020.3   Full year

  • 農業生物科学特別実験

    2019.4 - 2020.3   Full year

  • 農業生物科学演示技法

    2019.4 - 2020.3   Full year

  • 農業生物科学ティーチング演習

    2019.4 - 2020.3   Full year

  • 農業生物科学輪講

    2019.4 - 2020.3   Full year

  • 農業生物科学演習第二

    2018.10 - 2019.3   Second semester

  • 農業生物科学演習第一

    2018.10 - 2019.3   Second semester

  • ティーチング演習

    2018.4 - 2019.3   Full year

  • 農業生物科学特別実験

    2018.4 - 2019.3   Full year

  • 農業生物科学演示技法

    2018.4 - 2019.3   Full year

  • 農業生物科学ティーチング演習

    2018.4 - 2019.3   Full year

  • 農業生物科学輪講

    2018.4 - 2019.3   Full year

  • 農業生物科学プロジェクト演習

    2018.4 - 2019.3   Full year

  • 農業生物科学特別研究第一

    2018.4 - 2019.3   Full year

  • 農業生物科学特別研究第二

    2018.4 - 2019.3   Full year

  • 農業生物科学演習第一

    2017.10 - 2018.3   Second semester

  • 農業生物科学演習第二

    2017.10 - 2018.3   Second semester

  • 昆虫ゲノム科学演習

    2017.4 - 2018.3   Full year

  • 農業生物科学プロジェクト演習

    2017.4 - 2018.3   Full year

  • 農業生物科学特別研究第一

    2017.4 - 2018.3   Full year

  • 農業生物科学特別研究第二

    2017.4 - 2018.3   Full year

  • 昆虫分子遺伝学

    2017.4 - 2017.9   First semester

  • ゲノムサイエンスとエピジェネティクス

    2017.4 - 2017.9   First semester

  • 昆虫ゲノム科学演習

    2016.10 - 2017.3   Second semester

  • 農業生物資源学特別研究第二

    2016.4 - 2017.3   Full year

  • 農業生物資源学プロジェクト演習

    2016.4 - 2017.3   Full year

  • 農業生物資源学特別研究第一

    2016.4 - 2017.3   Full year

  • 昆虫分子遺伝学

    2016.4 - 2016.9   First semester

  • 蚕学演習

    2015.10 - 2016.3   Second semester

  • 農業生物資源学プロジェクト演習

    2015.4 - 2016.3   Full year

  • 農業生物資源学特別研究第一

    2015.4 - 2016.3   Full year

  • 農業生物資源学特別研究第二

    2015.4 - 2016.3   Full year

  • 昆虫分子遺伝学

    2015.4 - 2015.9   First semester

  • 蚕学演習

    2014.10 - 2015.3   Second semester

  • 農業生物資源学プロジェクト演習

    2014.4 - 2015.3   Full year

  • 農業生物資源学特別研究第一

    2014.4 - 2015.3   Full year

  • 農業生物資源学特別研究第二

    2014.4 - 2015.3   Full year

  • 昆虫分子遺伝学

    2014.4 - 2014.9   First semester

  • 蚕学演習

    2013.10 - 2014.3   Second semester

  • 農学実験第一

    2013.10 - 2014.3   Second semester

  • 農学実験第三

    2013.10 - 2014.3   Second semester

  • 農業生物資源学特別研究第一

    2013.4 - 2014.3   Full year

  • 農業生物資源学プロジェクト演習

    2013.4 - 2014.3   Full year

  • 農業生物資源学特別研究第二

    2013.4 - 2014.3   Full year

  • 昆虫機能利用学

    2013.4 - 2013.9   First semester

  • 昆虫分子遺伝学

    2013.4 - 2013.9   First semester

  • 農学実験第二

    2013.4 - 2013.9   First semester

  • 農学実験第一

    2012.10 - 2013.3   Second semester

  • 農学実験第三

    2012.10 - 2013.3   Second semester

  • 農学実験第二

    2012.4 - 2012.9   First semester

  • 昆虫機能利用学

    2012.4 - 2012.9   First semester

  • 昆虫ゲノム科学

    2010.10 - 2011.3   Second semester

  • 農学実験第三

    2010.10 - 2011.3   Second semester

  • 農学実験第一

    2010.10 - 2011.3   Second semester

  • 蚕学講究

    2010.4 - 2011.3   Full year

  • 遺伝育種学特別研究第二

    2010.4 - 2011.3   Full year

  • 蚕学演習第二

    2010.4 - 2011.3   Full year

  • 蚕学演習

    2010.4 - 2011.3   Full year

  • 農学実験第二

    2010.4 - 2010.9   First semester

  • トランスジェニック生物学概論

    2010.4 - 2010.9   First semester

  • 分子生物学実験

    2010.4 - 2010.9   First semester

  • 昆虫ゲノム科学

    2009.10 - 2010.3   Second semester

  • 農学実験第三

    2009.10 - 2010.3   Second semester

  • 農学実験第一

    2009.10 - 2010.3   Second semester

  • 蚕学講究

    2009.4 - 2010.3   Full year

  • 遺伝育種学特別研究第一

    2009.4 - 2010.3   Full year

  • 蚕学講究演習

    2009.4 - 2010.3   Full year

  • 蚕学演習第二

    2009.4 - 2010.3   Full year

  • 蚕学演習第一

    2009.4 - 2010.3   Full year

  • 農学実験第二

    2009.4 - 2009.9   First semester

  • 分子生物学実験

    2009.4 - 2009.9   First semester

  • 昆虫生理学

    2008.10 - 2009.3   Second semester

  • 農学実験第三

    2008.10 - 2009.3   Second semester

  • 農学実験第一

    2008.10 - 2009.3   Second semester

  • 分子生物学実験

    2008.4 - 2008.9   First semester

  • 農学実験第二

    2008.4 - 2008.9   First semester

  • 昆虫生理学

    2007.10 - 2008.3   Second semester

  • 農学実験第三

    2007.10 - 2008.3   Second semester

  • 農学実験第一

    2007.10 - 2008.3   Second semester

  • 分子生物学実験

    2007.4 - 2007.9   First semester

  • 農学実験第二

    2007.4 - 2007.9   First semester

  • 農学実験第一

    2006.10 - 2007.3   Second semester

  • 農学実験第三

    2006.10 - 2007.3   Second semester

  • 農学実験第二

    2006.4 - 2006.9   First semester

  • 分子生物学実験

    2006.4 - 2006.9   First semester

  • 農学実験第一

    2005.10 - 2006.3   Second semester

  • 農学実験第三

    2005.10 - 2006.3   Second semester

  • 農学実験第二

    2005.4 - 2005.9   First semester

  • 分子生物学実験

    2005.4 - 2005.9   First semester

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Social Activities

  • 文部科学省 サイエンスサイエンスハイスクール

    福岡県立小倉高校  2015.8

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    Audience: Infants, Schoolchildren, Junior students, High school students

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  • 文部科学省 サイエンスサイエンスハイスクール

    福岡県立小倉高校  2014.8

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    Audience: Infants, Schoolchildren, Junior students, High school students

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  • 文部科学省 サイエンスサイエンスハイスクール

    福岡県立小倉高校  2013.8

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    Audience: Infants, Schoolchildren, Junior students, High school students

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  • 文部科学省 サイエンスサイエンスハイスクール

    福岡県立小倉高校  2011.8

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    Audience: Infants, Schoolchildren, Junior students, High school students

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  • 文部科学省 サイエンスサイエンスハイスクール

    福岡県立小倉高校  2010.8

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    Audience: Infants, Schoolchildren, Junior students, High school students

    Type:Seminar, workshop

  • 文部科学省 サイエンスサイエンスハイスクール

    福岡県立小倉高校  2009.8

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    Audience: Infants, Schoolchildren, Junior students, High school students

    Type:Seminar, workshop

  • 文部科学省 サイエンスサイエンスハイスクール

    福岡県立小倉高校  2008.8

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    Audience: Infants, Schoolchildren, Junior students, High school students

    Type:Seminar, workshop

  • 文部科学省 サイエンスサイエンスハイスクール

    福岡県立小倉高校  2007.8

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    Audience: Infants, Schoolchildren, Junior students, High school students

    Type:Seminar, workshop

  • 文部科学省サイエンスパートナーシッププログラム 実験指導

    福岡高等学校  2006.8

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    Audience: Infants, Schoolchildren, Junior students, High school students

    Type:Seminar, workshop

  • 文部科学省スーパーサイエンスクラブ 実験指導

    小倉高等学校  2006.8

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    Audience: Infants, Schoolchildren, Junior students, High school students

    Type:Seminar, workshop

  • 文部科学省サイエンスパートナーシッププログラム 実験指導

    福岡県立修猷館高等学校  2005.8

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    Audience: Infants, Schoolchildren, Junior students, High school students

    Type:Seminar, workshop

  • 文部科学省スーパーサイエンスクラブ 実験指導

    福岡県立修猷館高等学校  2005.8

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    Audience: Infants, Schoolchildren, Junior students, High school students

    Type:Seminar, workshop

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