Updated on 2024/10/28

Information

 

写真a

 
NISHI OMI
 
Organization
Faculty of Agriculture Department of Bioresource Sciences Assistant Professor
Faculty of Agriculture Institute of Biological Control(Concurrent)
School of Agriculture Department of Bioresource and Bioenvironment(Concurrent)
Graduate School of Bioresource and Bioenvironmental Sciences Department of Bioresource Sciences(Concurrent)
Title
Assistant Professor
Homepage

Degree

  • 修士(農学)(九州大学 生資環修 第2387号)

  • 博士(農学)(九州大学 生資環博甲 第741号)

Research History

  • 2017年6月-2018年12月   農業・食品産業技術総合研究機構 野菜花き研究部門 野菜病害虫・機能解析研究領域 病害ユニット 契約研究員 2017年4月-2017年5月   国際農林水産業研究センター 水産領域 研究助手 2014年4月-2017年3月   日本学術振興会 (森林総合研究所) 特別研究員PD 2011年4月-2014年3月   日本学術振興会 (九州大学) 特別研究員DC1   

    2017年6月-2018年12月   農業・食品産業技術総合研究機構 野菜花き研究部門 野菜病害虫・機能解析研究領域 病害ユニット 契約研究員 2017年4月-2017年5月   国際農林水産業研究センター 水産領域 研究助手 2014年4月-2017年3月   日本学術振興会 (森林総合研究所) 特別研究員PD 2011年4月-2014年3月   日本学術振興会 (九州大学) 特別研究員DC1

Research Interests・Research Keywords

  • Research theme: Taxonomy and ecology of entomopathogenic fungi Microbial control of insect pests with entomopathogenic fungi

    Keyword: entomopathogenic fungi, soil microbiology, phylogenetics, termite、host selectivity

    Research period: 2019.1

Awards

  • 第32回日本菌学会奨励賞

    2023.5   日本菌学会   Phylogenetic classification and physiological and ecological traits of Metarhizium spp.

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    Metarhizium属は冬虫夏草類などが所属する子嚢菌門のボタンタケ目バッカクキン科に属し、記載種の多くが昆虫寄生性である。本属は宿主範囲が多様な種を含み、広宿主域の黒きょう病菌M. anisopliaeを主として一部の種が害虫防除資材として利用されてきた。本属は昆虫病原性だけでなく、温度特性や紫外線耐性、および生息環境選好性においても多様であることが報告され、それらは害虫防除資材としての有用性に関わる重要な特性として認識されてきた。一方で本属はDNA情報に基づく分子系統学的手法による分類の改訂が進行し、部分的にはGCPSR(Genealogical Concordance Phylogenetic Species Recognition)による系統的種の識別という考えに基づき細分された。しかし、この分類体系は害虫防除において重要なM. anisopliae complexなどの分類群においては、多様な宿主昆虫関連性や生理生態学的特性を整理する枠組みとしてはあまり機能しなかった。すなわち、種に対応させられた単系統は宿主や地理的由来などの分離源情報や形態的特徴との対応は認められず(それぞれの単系統が多様な宿主昆虫や地理的由来の菌株を含み)、また生理学的特性や生息環境タイプとの対応は未調査であった。国内においては害虫防除における有用性から菌株が収集されてきたが、DNA情報に基づいた同定がされておらず、種数および系統学的多様性が未解明であった。そこで、演者は形態的区別が困難な種が多く含まれるM. anisopliae complexおよびM. flavoviride complexを中心として、国内の菌株を収集し分子系統学的手法により同定し、単系統ごとの温度特性、生息環境選好性、および昆虫病原性における特徴の解明を試みた。
    まず国内の土壌および昆虫から分離された本属の菌株が、伸長因子1α領域の分子系統解析において9既知種の単系統に所属することを明らかにした。この結果から、これまで形態的特徴に基づいてM. anisopliaeに同定されていた国内株の多くがM. pingshaenseの単系統に所属することも明らかとなった。また既知種の単系統との塩基配列の乖離が大きい菌株が2群見出された。これらは複数遺伝子座の解析により最近縁種との系統学的独立性が認められ、かつ形態的な分化も認められたので、それぞれM. bibionidarumおよびM. purpureogenumとして記載した。M. bibionidarumはケバエ科Bibionidaeの幼虫から分離され、本種の単系統に所属する菌株は、姉妹種にあたるM. pemphigiよりも分生子が大きい傾向が認められた。M. purpureogenumは土壌から平板法やヤマトシロアリを用いた釣り出し法により分離された。本種は寒天培養時に菌叢の周囲が赤紫色に着色する特徴を示した。
    温度特性に関して、M. brunneumとM. pemphigiの単系統に所属する菌株は他の菌株と比較して、分生子の発芽が低温で早く、高温で遅いという明瞭な低温型の生育特性を示すことを明らかにした。国外株においても同じ傾向が認められた。次に単系統ごとの生息環境における傾向を明らかにするため、森林や耕作地などの土壌から本属を分離し、環境タイプごとの検出頻度を調査した。まず大量菌株の同定を効率化するためPCR-RFLPマーカーを作成した。rDNAのIGS領域は粗精製DNAからでもシングルバンドの増幅成功率が高く、かつ制限酵素HaeIIIによる消化後の電気泳動パターンにより、種内多型を検出可能であった。このDNAマーカーを用いて全国で採集した土壌からの各種の検出頻度を調べた結果、どの環境タイプからもM. pingshaenseの検出頻度が最も高かったが、低温型の生育を示した2種は他の種と比較して森林環境に偏って分布する傾向が認められた。福岡県糟屋郡の立花山周辺における集中的サンプリング調査でも、この3種に関して同様の分布傾向が認められた。低温型の2種は複数目の昆虫から分離されており宿主範囲は広いと考えられるので、この分布は林床の冷涼な温度環境への適応と関連することが示唆された。さらに同様の手法で野草根圏における分布傾向の解明も試みた。茨城県つくば市の野草根圏から本属5種が検出され、野草の分類群にかかわらずM. pingshaenseが最も高頻度で検出されることを明らかにした。
    病原性に関して、接種試験によりシロテンハナムグリに対する病原性を評価し、M. majusの単系統に所属するシロテンハナムグリ分離株とそれと同一の末端節単系統を構成する土壌分離株のみが病原性を示すことを明らかにした。これらの菌株はカイコガに対する病原力は比較的弱い傾向があり、シロテンハナムグリに特化した病原性をもつと考えられた。本種の単系統にはコガネムシ科分離株が多数所属し、かつそれらはシロテンハナムグリ分離株と類似した大型分生子をもつことから、宿主分類群と形態的特徴に共通性が認められたが、病原性はそれより下位の末端節レベルで分化していることが示された。
     本研究により、それまでほぼ未解明であった日本のMetarhizium属糸状菌の系統学的多様性と単系統ごとの生理生態学的特徴の一端を明らかにした。特に温度特性に関しては、単系統と生理特性との明瞭な関係が国外株も含めて示され、GCPSRに基づく系統学的分類の有用性を再確認することにもなった。今後は宿主昆虫と関連する末端節単系統の特性評価と独立系統性の検証を行い、分類学的位置付けを明確にするとともに、宿主昆虫の寄主植物と関連した生理生態学的特性などの新たな評価軸も加えて本属の多様性と単系統ごとの特徴の解明を進めたいと考えている。

  • 生物資源環境科学府賞

    2011.3   九州大学   修士論文「日本の土壌から分離したMetarhizium属糸状菌の系統解析」

Papers

  • Phylogenetic classification and physiological and ecological traits of Metarhizium spp. Invited Reviewed International journal

    Nishi O.

    Mycoscience   65 ( 5 )   235 - 243   2024   ISSN:13403540

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    Authorship:Lead author, Last author, Corresponding author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:Mycoscience  

    The genus Metarhizium (Hypocreales: Clavicipitaceae) is mostly composed of entomopathogenic fungal species. Many of these species are anamorphic and difficult to distinguish morphologically. Furthermore, most isolates of this genus have a broad host range, making classification based on host-insect species uncertain. Molecular phylogenetic analysis based on DNA sequence information distinguishes these species well and revises the taxonomy of Metarhizium. However, in the revisions, the major groups within the genus, such as M. anisopliae complex, were classified regardless of their phenotypic differentiation. Therefore, the characteristics of the individual species remain unclear. To explore the species-specific characteristics of Metarhizium spp., the author performed a phylogenetic analysis and characterization of Metarhizium spp. in Japan. The results showed that strains of the M. brunneum and M. pemphigi clades exhibited cold-active growth characteristics and preferred forested environments over M. pingshaense. In the M. majus clade, a specialist of scarab beetles, isolates from different Scarabaeidae species, including the coconut rhinoceros beetle (Oryctes spp.) and flower chafer beetle (Protaetia orientalis), formed separate subclades and showed strong virulence against their original hosts. This review describes the current state of understanding of the taxonomy and species-specific characteristics of the genus Metarhizium, and includes the author’s own previous study.

    DOI: 10.47371/mycosci.2024.07.001

    Scopus

  • Isolation of Metarhizium spp. from rhizosphere soils of wild plants reflects fungal diversity in soil but not plant specificity Reviewed International journal

    NISHI Oumi*, SATO Hiroki

    Mycology   10 ( 1 )   22 - 31   2018.9

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1080/21501203.2018.1524799

    Other Link: https://www.tandfonline.com/doi/citedby/10.1080/21501203.2018.1524799?scroll=top&needAccess=true

  • Abundance of the soil entomopathogenic fungus Metarhizium anisopliae sensu lato in agricultural field and forest soils in Japan Reviewed International journal

    NISHI Oumi*, IIYAMA Kazuhiro, YASUNAGA-AOKI Chisa, SHIMIZU Susumu

    Current Research in Environmental & Applied Mycology   8 ( 4 )   468 - 473   2018.8

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.5943/cream/8/4/6

    Other Link: http://www.creamjournal.org/volume-8/issue-4.html#article223

  • Species diversity of the entomopathogenic fungi Metarhizium anisopliae and M. flavoviride species complexes isolated from insects in Japan Reviewed International journal

    Oumi Nishi*, Hiroki Sato

    Mycoscience   58 ( 6 )   472 - 479   2017.11

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    Language:English   Publishing type:Research paper (scientific journal)  

    Phylogenetic analyses of insect-derived isolates of the Metarhizium anisopliae and M. flavoviride species complexes in Japan were conducted to reveal their species diversity. Fifty-seven isolates were identified as nine species, including one species first reported for Japan. Metarhizium pingshaense was the most frequently isolated species from this genus, and the 29 isolates of M. pingshaense came from six orders and 14 families of insects. New host–pathogen associations were found for two species with relatively narrow host ranges: Hymenoptera-M. pemphigi, Orthoptera- and Phasmatodea-M. majus.

    DOI: 10.1016/j.myc.2017.06.008

  • Metarhizium bibionidarum and M. purpureogenum new species from Japan Reviewed International journal

    Oumi Nishi*, Susumu Shimizu, Hiroki Sato

    Mycological Progress   16 ( 10 )   987 - 998   2017.10

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    Language:English   Publishing type:Research paper (scientific journal)  

    Two new species of Metarhizium, M. bibionidarum and M. purpureogenum are described from Japan. Metarhizium bibionidarum is the phylogenetic sister species of M. pemphigi and a member of the M. flavoviride species complex. It is distinguished morphologically from M. pemphigi by its larger conidia. The species is based on a collection of an infected March fly larva (Diptera: Bibionidae) but is also known to occur on fruit beetle (Coleoptera: Scarabaeidae) encountered in France. Metarhizium purpureogenum was isolated from soil by plating and insect baiting methods and represents a unique phylogenetic lineage placed outside the M. anisopliae and M. flavoviride species complexes. Three isolates of M. purpureogenum excreted a distinctive red-purple pigment into agar medium when co-cultured with M. robertsii or Aspergillus oryzae.

    DOI: 10.1007/s11557-017-1333-x

  • Species associations and distributions of soil entomopathogenic fungi Metarhizium spp. in Japan Reviewed International journal

    Oumi Nishi*, Kazuhiro Iiyama, Chisa Yasunaga-Aoki, Susumu Shimizu

    Mycology   8 ( 4 )   308 - 317   2017.10

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    Language:English   Publishing type:Research paper (scientific journal)  

    Metarhizium Sorokīn (Hypocreales: Clavicipitaceae) is a genus of facultative parasites of insects found in soils from various environments and is used for pest management. Understanding the habitat selection of Metarhizium spp. is critical to improve the efficacy and persistence of these fungi as microbial insecticides. This study sought to determine the habitat preferences of Metarhizium spp. in Japan. We identified 302 isolates of Metarhizium spp. as eight species by a combination of PCR–RFLP and phylogenetic analysis of DNA sequences. M. pingshaense was the predominant species in Japan and was most frequently isolated from both forest and agricultural environments. On the other hand, M. brunneum and M. pemphigi were comparatively restricted to forest environments. A similar species association was detected in a small area that was intensively investigated, where 7 species including 14 genotypes were isolated from soil. The results of this study have revealed different habitat preferences among Metarhizium spp. in Japan.

    DOI: 10.1080/21501203.2017.1386244

  • Phylogenetic status and pathogenicity of Metarhizium majus isolated from a fruit beetle larva in Japan Reviewed International journal

    Oumi Nishi*, Kazuhiro Iiyama, Chisa Yasunaga-Aoki, Susumu Shimizu

    Mycological Progress   14 ( 8 )   2015.8

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    Language:English   Publishing type:Research paper (scientific journal)  

    Some isolates of Metarhizium anisopliae sensu lato with large conidia (MALC), which was formerly known as M. anisopliae var. majus, from a scarabaeid host, such as isolates from the coconut rhinoceros beetle (Oryctes spp.), have a narrow host range and are particularly adapted to their original host. Despite recent taxonomic revisions of the genus Metarhizium on the basis of DNA sequences, variations in host preferences of MALC have not been linked to DNA sequence variations. This study focused on the phylogenetic status and pathogenicity of MALC isolated from the fruit beetle larva Protaetia orientalis submarumorea (Pos) in Japan to investigate the relationship between virulence and nucleotide sequence variation among MALC. On the basis of the results of the phylogenetic analyses of DNA sequences from five loci, we identified an isolate from Pos (Hn1) as M. majus, which comprised many isolates with large conidia from scarabaeid insects. In the virulence assay of genetically diverse Metarhizium isolates, only Hn1 and its most closely related isolates from soil showed pathogenicity to Pos; however, these Pos-pathogenic isolates showed weak virulence against silkworms. In the analysis of the intergenic spacer region of rDNA, the Pos-pathogenic isolates displayed unique sequence variation and were clearly distinguished from closely related lineages, including isolates from Oryctes sp., which formed a separate monophyletic group. These results indicate that the Pos-pathogenic isolates from Japan are particularly adapted to Pos and can be genetically distinguished from M. majus isolates from different scarabaeid insects.

    DOI: 10.1007/s11557-015-1082-7

  • Comparison of the germination rates of Metarhizium spp. conidia from Japan at high and low temperatures Reviewed International journal

    Oumi Nishi*, Kazuhiro Iiyama, Chisa Yasunaga-Aoki, S. Shimizu

    Letters in Applied Microbiology   57 ( 6 )   554 - 560   2013.12

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    Language:English   Publishing type:Research paper (scientific journal)  

    The knowledge of the growth characteristics of entomopathogenic fungi at different temperatures is very important for understanding their ecology and field efficacy as biological control agents. However, the relationships between their DNA-based phylogenetic tree classifications and growth characteristics at different temperatures have not been adequately investigated. In this study, we compared the phylogenetic relationships between Metarhizium anisopliae sensu lato and M. flavoviride isolates obtained from soils in various environments in Japan and the germination rates of their conidia on agar medium in hot and cold conditions. The results showed that the 89 Japanese isolates belonged to the clade of eight species, according to the molecular phylogenetic analysis. The germination rates of isolates belonging to the M. brunneum and M. flavoviride var. pemphigi clades were higher at lower temperatures (9·7-11·0°C) and lower at higher temperatures (34·3-35·2°C) compared with the other six species. The isolates of these two species originated from different geographical regions in Japan, despite their uniform germination characteristics. This study detected the clear interspecific differences in the in vitro germination characteristics of the Japanese isolates of Metarhizium spp. at two different temperature regimes.

    DOI: 10.1111/lam.12150

  • Phylogenetic analysis of Metarhizium spp. isolated from soil in Japan Reviewed International journal

    Oumi Nishi, Keiichi Hasegawa, Kazuhiro Iiyama, Chisa Yasunaga-Aoki, Susumu Shimizu*

    Applied Entomology and Zoology   46 ( 3 )   301 - 309   2011.8

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    Language:English   Publishing type:Research paper (scientific journal)  

    As a result of analyzing the internal transcribed spacer (ITS) and 5′ end of the EF-1α sequence of 145 isolates of Metarhizium spp. isolated from soil in Japan using selective agar medium, eight species were identified. ITS sequence analysis divided the isolates into three clades. Two were identified as M. flavoviride var. pemphigi and M. lepidiotae, respectively. EF-1α sequence analysis identified the other clades as six species: M. anisopliae, M. brunneum, M. guizhouense, M. majus, M. pingshaense and M. robertisii. The distribution of M. flavoviride var. pemphigi was restricted to forest or wood soil, and conidial sizes of M. guizhouense and M. majus were incongruent with the phylogeny based on the sequence of the 5′ end of EF-1α.

    DOI: 10.1007/s13355-011-0045-y

  • ヤマトシロアリReticulitermes speratus を用いた釣り出し法による土壌からの昆虫病原性糸状菌の分離 Reviewed

    西大海,飯山和弘,青木智佐,清水進*

    Entomotech   35   21 - 26   2011.3

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    Language:Japanese   Publishing type:Research paper (scientific journal)  

  • Incongruence of EF-1α phylogeny and morphology between Metarhizium guizhouense and M. majus Reviewed

    Oumi Nishi, Kazuhiro Iiyama, Chisa Yasunaga-Aoki, S. Shimizu*

    Entomotech   34   19 - 23   2010.2

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    Language:English   Publishing type:Research paper (scientific journal)  

  • Virulence of Metarhizium anisopliae var. majus isolated from soils against termite, Coptotermes formosanus Shiraki Reviewed

    Oumi Nishi, Kazuhiro Iiyama, Chisa Yasunaga-Aoki, S. Shimizu*

    Japanese Journal of Environmental Entomology and Zoology   21   29 - 35   2010.1

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    Language:English   Publishing type:Research paper (scientific journal)  

  • 九州の土壌から分離したMetarhizium 属糸状菌の系統解析 Reviewed

    西大海,飯山和弘,青木智佐,清水進*

    Entomotech   33   49 - 53   2009.2

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    Language:Japanese   Publishing type:Research paper (scientific journal)  

  • 野外鱗翅目昆虫に蔓延する微胞子虫病とその宿主個体群への影響

    @青木智佐, @和佐野直也, @西大海

    昆虫と自然   786   36 - 38   2024.1

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    Language:Japanese   Publishing type:Research paper (scientific journal)  

  • Entomopathogenic fungus Beauveria bassiana–based bioinsecticide suppresses severity of powdery mildews of vegetables by inducing the plant defense responses Reviewed International journal

    Iida Y, Higashi Y, Nishi O, Kouda M, Maeda K, Yoshida K, Asano S, Kawakami T, Nakajima K, Kuroda K, Tanaka C, Sasaki A, Kamiya K, Yamagishi N, Fujinaga M, Terami F, Yamanaka S and Kubota M

    Front. Plant Sci.   14   1211825   2023.8   ISSN:1664-462X

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Frontiers in Plant Science  

    The entomopathogenic fungus Beauveria bassiana is used commercially as a microbial insecticides against a wide range of agricultural insect pests. Some strains of B. bassiana protect the plants from pathogens, but the underlying mechanisms are largely unknown. Here, we found that prophylactic sprays of commercial bioinsecticide Botanigard on cucumber, tomato, and strawberry plants suppressed the severity of economically damaging powdery mildews. On leaf surfaces, hyphal elongation and spore germination of cucumber powdery mildew, Podosphaera xanthii , were inhibited, but B. bassiana strain GHA, the active ingredient isolated from Botanigard, only inhibited hyphal elongation but had no effect on spore germination of P. xanthii . In addition, strain GHA suppressed powdery mildew symptoms locally, not systemically. Treatment with Botanigard and strain GHA induced a hypersensitive response (HR)–like cell death in epidermal cells of the cucumber leaves in a concentration-dependent manner and inhibited penetration by P. xanthii . Transcriptome analysis and mass spectrometry revealed that GHA induced expression of salicylic acid (SA)–related genes, and treatment with Botanigard and GHA increased the SA level in the cucumber leaves. In NahG -transgenic tomato plants, which do not accumulate SA, the biocontrol effect of tomato powdery mildew by GHA was significantly reduced. These results suggested that B. bassiana GHA induces SA accumulation, leading to the induction of HR-like cell death against powdery mildew and subsequent suppression of fungal penetration. Thus, Botanigard has the potential to control both insect pests and plant diseases.

    DOI: 10.3389/fpls.2023.1211825

    Web of Science

    Scopus

    PubMed

  • Varied selectivity of caterpillar‐specific Metarhizium rileyi and generalist entomopathogenic fungi against last instar larvae and pupae of common cutworm, Spodoptera litura (Lepidoptera: Noctuidae) Reviewed International journal

    #Mulue Girmay Gebreslasie,@Oumi Nishi,@Naoya Wasano,@Chisa Yasunaga‐Aoki

    Applied Entomology and Zoology   58 ( 3 )   219 - 228   2023.4   ISSN:0003-6862 eISSN:1347-605X

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Applied Entomology and Zoology  

    Spodoptera litura (Fabricius) (Lepidoptera: Noctuidae) is a serious pest of crops and vegetables in several Asian areas. Metarhizium rileyi is one of the most promising entomopathogenic fungi for controlling S. litura because of its specificity to lepidopteran larvae; however, little is known about its virulence against last instar larvae and pupae of S. litura although these stages are efficiently controlled by introducing entomopathogenic fungi into the soil. This study aimed to characterize the virulence of M. rileyi strain against the two stages of S. litura by comparing it with 13 strains of entomopathogenic fungi with broad host ranges. In inter-stage virulence comparisons, most fungal strains showed considerably stronger virulence against larvae than against pupae, with M. rileyi Nr4 showing the largest difference. Inter-strain virulence comparisons based on final mortality and mycosis proportion as well as integrative virulence scores revealed that M. rileyi Nr4 was the most virulent against larvae, whereas it was the weakest against pupae, but caused malformation in emerged adults. These results suggest that M. rileyi Nr4 is the most promising for controlling the last instar larvae and is less effective against pupae than the generalist fungi.

    DOI: 10.1007/s13355-023-00824-x

    Web of Science

    Scopus

  • Entomopathogenic fungus Akanthomyces muscarius (Hypocreales: Cordycipitaceae) strain IMI 268317 colonises on tomato leaf surface through conidial adhesion and general and microcycle conidiation Reviewed International journal

    @西 大海, @須志田浩稔, @東佑実子, @飯田祐一郎

    Mycology   13 ( 2 )   133 - 142   2022.6   ISSN:2150-1203 eISSN:2150-1211

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Mycology  

    The entomopathogenic fungus Akanthomyces muscarius strain IMI 268317, previously known as Lecanicilliummuscariumand Verticillium lecanii, is currently used as a microbial insecticide to protect tomatoes from serious leaf-inhabiting pests in greenhouses. However, its persistence on tomato leaves has been unidentified. Understanding the events and processes of phyllosphere colonisation by this strain should help in developing its practical applications. This study assessed the epiphytic abilities of this strain on tomato leaves in humid conditions, simulating closed greenhouse environments. Conidia applied on tomato leaflets strongly adhered 12 h after inoculation. The mucilage-like materials were found around the germinated conidia after 3 days after inoculation (dpi), which possibly strengthened the adhesion. A total of 15% of conidia germinated at 3 dpi, of which 2% formed typical conidium or an enlarged structure on germ-tube tips. Many conidia were produced on phialide tips that branched from elongated hyphae at 7 dpi; however, invasion into leaf tissue was not observed. On the leaflets, inoculated conidia suspensions of 1 × 105 and 1 × 106 conidia/mL, colony forming units increased 52.6 and 8.8 folds from 0 to 14 dpi, respectively. These results suggested that A. muscarius strain IMI 268317 has high epiphytic abilities on tomato leaflets in a humid condition.

    DOI: 10.1080/21501203.2021.1944929

    Web of Science

    Scopus

    PubMed

  • Biocontrol Activity of Nonpathogenic Strains of <i>Fusarium oxysporum</i>: Colonization on the Root Surface to Overcome Nutritional Competition

    Iida, Y; Ogata, A; Kanda, H; Nishi, O; Sushida, H; Higashi, Y; Tsuge, T

    FRONTIERS IN MICROBIOLOGY   13   2022.1   eISSN:1664-302X

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    Publisher:Frontiers in Microbiology  

    Fusarium oxysporum is a soil-borne fungal pathogen that causes vascular wilts in a wide variety of crops. Certain nonpathogenic strains of F. oxysporum are known to protect crops against F. oxysporum pathogens. We assessed the biocontrol activities of nonpathogenic mutants of F. oxysporum ff. spp. melonis and lycopersici generated by disruption of the FOW2 gene, which encodes a Zn(II)2Cys6-type transcriptional regulator essential for their pathogenicity. Pre-inoculation of melon or tomato roots with strain ΔFOW2 conidia markedly reduced disease incidence caused by the parental wild-type strain in a concentration-dependent manner of conidial suspensions of ΔFOW2 strains. The biocontrol effect caused by the ΔFOW2 pre-inoculation lasted for at least 7 days. Pre-inoculation of melon roots with the wild-type or ΔFOW2 strain of F. oxysporum f. sp. lycopersici and nonpathogenic F. oxysporum strain also led to biocontrol activity against F. oxysporum f. sp. melonis, indicating that the biocontrol activity of ΔFOW2 strains is due to its nonpathogenic nature, not to the FOW2 disfunction. Conidial germination and hyphal elongation of only the wild-type strain were inhibited on melon root surface pre-inoculated with conidia of strains nonpathogenic to melon plants. Expression of defense-related genes was not significantly induced in roots and aboveground parts of melon seedlings preinoculated with ΔFOW2 conidia. Carbon source competition assay showed that nonpathogenic strains competed with the wild-type strain for a carbon source in soil. Strain ΔFOW2 also competed with the oomycete pathogen Pythium aphanidermatum for carbon source and protected melon plants from P. aphanidermatum. Our results suggest that the biocontrol activity of the nonpathogenic F. oxysporum strains used in this study mainly depends on their extensive colonization of the root surface and outcompeting pathogens for nutrients.

    DOI: 10.3389/fmicb.2022.826677

    Web of Science

    Scopus

    PubMed

  • Biocontrol Activity of Nonpathogenic Strains of Fusarium oxysporum: Colonization on the Root Surface to Overcome Nutritional Competition Reviewed International journal

    @Iida Y*, @Ogata A, @Kanda H, @Nishi O, @Sushida H, @Higashi @Y, Tsuge T

    Frontiers in Microbiology   13   826677   2022.1

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    Language:Japanese   Publishing type:Research paper (scientific journal)  

  • 昆虫寄生菌の土壌における生態特性と潜土性チョウ目害虫の防除への展望 特集「チョウ目病原微生物および線虫をめぐる研究動向と害虫防除利用への展望」) Invited

    @西 大海

    蚕糸・昆虫バイオテック   90 ( 2 )   57 - 66   2021.9

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    Language:Japanese   Publishing type:Research paper (scientific journal)  

  • 昆虫病原性鞭毛虫と害虫防除利用への展望 特集「チョウ目病原微生物および線虫をめぐる研究動向と害虫防除利用への展望」 Invited

    #鈴木翔太, @青木智佐, @西大海

    蚕糸・昆虫バイオテック   90 ( 2 )   79 - 86   2021.9

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    Language:Japanese   Publishing type:Research paper (scientific journal)  

  • Epiphytic and endophytic colonisation of tomato plants by the entomopathogenic fungus Beauveria bassiana strain GHA Reviewed International journal

    @西 大海, @須志田浩稔, @東佑実子, @飯田祐一郎

    Mycology   12 ( 1 )   39 - 47   2021.1

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    Language:Japanese   Publishing type:Research paper (scientific journal)  

    Beauveria bassiana, known for its entomopathogenic characteristics, is the most widely used biocontrol agent against many insect pests and may also be active against soil-borne pathogens. It inhabits the surfaces or inner tissues of various plant species without causing any visible signs or symptoms. Here we show that B. bassiana strain GHA, the active ingredient of a commercial microbial insecticide, colonises tomato plants. GHA grew on intact leaf surfaces of tomato in high humidity, but never entered stomata. Viable hyphae and conidia were detected, and the population on inoculated leaves significantly increased until 14 days after inoculation. On tomato leaves, GHA conidiated normally via conidiophores and phialides, and also via microcycle conidiation (conidiophores and phialides form directly from germ tubes and produce conidia). Hyphae were also detected inside the rachis, even more frequently after plant surfaces were scarified. These results suggested that B. bassiana strain GHA can grow epiphytically and endophytically on tomato plants.

    DOI: 10.1080/21501203.2019.1707723

  • Identification and characterization of three microsporidian genera concurrently infecting a silkworm, Bombyx mori, in Brazil Reviewed International journal

    Maximiano Corrêa Cassal, Airi Fukushima, Oumi Nishi, Kazuhiro Iiyama, Lidia Mariana Fiuza, Chisa Yasunaga-Aoki

    Journal of Invertebrate Pathology   177   107502   2020.11

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.jip.2020.107502

  • Detection of cry-type gene homologues in the draft genome sequence of Paenibacillus popilliae ATCC 14706T and trial for the expression of recombinant proteins in Escherichia coli Reviewed International journal

    #川原顕生, @飯山和弘, @浅野眞一郎, @西 大海, @青木智佐

    Journal of Insect Biotechnology and Sericology   89   25 - 30   2020.1

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    Language:English   Publishing type:Research paper (scientific journal)  

  • Evaluation of the potential biocontrol activity of Dicyma pulvinata against Cladosporium fulvum, the causal agent of tomato leaf mold Reviewed International journal

    Iida Y*, Ikeda K, Sakai H, Nakagawa H, Nishi O, Higashi Y

    Plant Pathology   67 ( 9 )   1883 - 1890   2018.12

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1111/ppa.12916

    Other Link: https://onlinelibrary.wiley.com/doi/full/10.1111/ppa.12916

  • Abundance of the soil entomopathogenic fungus Metarhizium anisopliae sensu lato in agricultural field and forest soils in Japan Reviewed International journal

    @Oumi Nishi*, @K. Iiyama, @C. Yasunaga-Aoki, @S. Shimizu

    Current Research in Environmental and Applied Mycology   8 ( 4 )   468 - 473   2018.1

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    Language:English   Publishing type:Research paper (scientific journal)  

    Metarhizium anisopliae sensu lato is a facultative entomopathogenic, soil-inhabiting fungus that has been used for biological control of soil-dwelling arthropod pests. However, little is known about the density of M. anisopliae sensu lato in forest soils and the difference in the density among different habitat types and geographical origins. In this study, we determined the density of this fungus in soil samples collected from forest and agricultural fields in Japan by plating method with semi-selective agar medium and analyzed its associations with the habitat types and latitudes of the collecting sites. Mean and mode density of 211 soil samples were 9.4 × 10 2 CFUs/g soil and 1.0 × 10 3 -1.0 × 10 4 CFUs/g soil, respectively, which were comparable to the levels previously reported in other countries. The density in forest soil was not significantly different from that of the other habitat types, despite previous studies that concluded M. anisopliae sensu lato to be less abundant in forest soil based on occurrence determined by insect bait method. The latitude was also not significantly associated with the density. This study revealed higher abundance of M. anisopliae sensu lato in forest soil than the original expectation.

    DOI: 10.5943/CREAM/8/4/6

  • Production and characterization of the celery mismatch endonuclease CEL II using baculovirus/silkworm expression system Reviewed International journal

    Hiroaki Mon, Man Lee, Mai Fukushima, Yudai Nagata, Mie Fujii, Jian Xu, Omi Nishi, Kazuhiro Iiyama, Takahiro Kusakabe*

    Applied Microbiology and Biotechnology   97 ( 15 )   6813 - 6822   2013.8

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    Language:English   Publishing type:Research paper (scientific journal)  

    Mutation and polymorphism detection by nucleases has become a more important tool in clinical and biological researches. There are several kinds of single-stranded nucleases for detecting mismatched DNAs. One of them, CEL II, was isolated from Apium graveolens and cleaves DNA with high specificity at sites of mismatch. High-throughput mutation scanning requires large quantity of CEL II endonuclease. Here, we demonstrate high-level expression of CEL II using silkworm-baculovirus system. The recombinant CEL II secreted in silkworm hemolymph was glycosylated and susceptible to N-glycosidase F. Additionally, larger metal ions such as Ca2+ and Sr2+ were able to replace Mg2+ and enhanced mismatch cleavage activity of CEL II. These results indicate that the silkworm-baculovirus platform is a good alternative system to obtain the functional CEL II.

    DOI: 10.1007/s00253-012-4583-1

  • Phylogenetic analysis of Paenibacillus popilliae and its related taxa based on housekeeping genes Reviewed International journal

    Kazuhiro Iiyama*, Oumi Nishi, Hiroaki Mon, Man Lee, Takahiro Kusakabe, Shin Ichiro Asano, Chisa Yasunaga-Aoki, Susumu Shimizu

    Journal of Insect Biotechnology and Sericology   82 ( 1 )   1 - 11   2013.8

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    Language:English   Publishing type:Research paper (scientific journal)  

    Four housekeeping genes including gapA, groEL, gyrA and pgi were used in order to reveal a phylogenetic relationship among Paenibacillus species. Phylogenetic analysis and end-to-end pairwise analysis were carried out using nucleotide and amino acid sequences of the housekeeping genes. A monophylogenetic clade including P. popilliae, P. thiaminolyticus and P. dendritiformis formed in all phylogenetic trees. The results in both analyses indicated that the relationship between P. larvae subsp. larvae and P. popilliae was comparatively far. The result of the end-to-end pairwise analysis also showed that, among the Paenibacillus species used in this study, the closest species to P. popilliae was P. dendritiformis.

    DOI: 10.11416/jibs.82.1_001

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Presentations

  • 白きょう病菌Beauveria bassiana GHA株における病原力遺伝子破壊株の作製

    @西大海, #周雪, @和佐野直也, @青木智佐

    第68回日本応用動物昆虫学会大会  2024.3 

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    Event date: 2024.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:仙台国際センター(宮城県仙台市)   Country:Japan  

  • Metarhizium属菌の分子系統学的分類と生理生態学的特性 Invited

    @西大海

    日本菌学会第67回大会  2023.5 

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    Event date: 2023.5 - 2022.5

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Venue:熊本県民交流会館パレア10階(熊本県熊本市中央区手取本町8-9)   Country:Japan  

  • メタリジウム菌の既知ゲノム情報を利用した高多型領域の選定と種内系統解析

    @西大海, @和佐野直也, @青木智佐

    第66回日本応用動物昆虫学会大会  2022.3 

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    Event date: 2022.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:明治大学(オンライン開催)   Country:Japan  

  • 昆虫寄生菌のミカンキイロアザミウマに対する病原性とその土壌混合による影響

    @西 大海, #松岡拓, @青木智佐

    第65回日本応用動物昆虫学会大会  2021.3 

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    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:島根大学(オンライン)   Country:Japan  

  • ナミアゲハとNosema bombycisのゲノム比較から見い出された新知見

    @和佐野直也、@西大海、@青木智佐

    日本蚕糸学会第94回大会  2024.3 

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    Event date: 2024.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:農林水産技術会議事務局筑波産学連携支援センター本館(茨城県つくば市観音台2-1-9)   Country:Japan  

  • ベニシジミ由来微胞子虫の単離とカイコに対する病原性

    @和佐野直也、@西大海、@青木智佐

    日本蚕糸学会第94回大会  2024.3 

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    Event date: 2024.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:農林水産技術会議事務局筑波産学連携支援センター本館(茨城県つくば市観音台2-1-9)   Country:Japan  

  • Metarhizium属の広宿主域種群の単系統に所属するエンマコオロギ属に選択的な菌株において複数の既知病原力遺伝子が欠失している可能性について

    @西大海,@和佐野直也,@青木智佐

    日本菌学会第66回大会  2022.8 

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    Event date: 2022.8

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:オンライン(大阪市立自然史博物館)   Country:Japan  

  • ナミアゲハゲノム上から見出される微胞子虫遺伝子群

    @和佐野直也、@西大海、@青木智佐

    日本蚕糸学会第92回大会  2022.3 

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    Event date: 2022.6

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:オンライン   Country:Japan  

  • Induratia fengyangensis volatile compounds on West Indian sweet potato weevil, Euscepes postfasciatus (Fairmaire) International conference

    #Chui-Chai, @N., Nishi, @O., Wasano, N., @Yasunaga-Aoki

    International Congress on Invertebrate Pathology and Microbial Control & 53rd Annual Meeting of the Society for Invertebrate Pathology  2021.6 

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    Event date: 2021.6

    Language:English   Presentation type:Oral presentation (general)  

    Venue:オンライン   Country:Japan  

  • カイコ病原性鞭毛虫Leptomomas sp.の同定および諸性状の再検討

    #鈴木翔太, @西 大海, @青木智佐

    令和2年度 蚕糸・昆虫機能利用学術講演会 日本蚕糸学会第90回大会  2020.3 

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    Event date: 2020.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:信州大学 繊維学部   Country:Japan  

  • Study on germination inhibitor of Nosema bombycis spores contained in metabolites of Enterococcus sp. isolated from Bombyx mori

    #王 敏玉, @西 大海, @青木智佐

    令和2年度 蚕糸・昆虫機能利用学術講演会 日本蚕糸学会第90回大会  2020.3 

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    Event date: 2020.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:信州大学 繊維学部   Country:Japan  

  • カイコ微粒子病病原Nosema bombycisの異なるカイコ系統における性状比較

    #福島藍里, @青木智佐, @西 大海

    令和2年度 蚕糸・昆虫機能利用学術講演会 日本蚕糸学会第90回大会  2020.3 

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    Event date: 2020.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:信州大学 繊維学部   Country:Japan  

  • オオホシカメムシ科2種の死体から分離された糸状菌の同定

    @◯西 大海

    日本菌学会第63回大会  2019.5 

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    Event date: 2019.5

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:秋田県立大学   Country:Japan  

    オオホシカメムシおよびヒメホシカメムシ(カメムシ目オオホシカメムシ科)は日本では本州以南に広く分布し、アカメガシワの花穂などに集まる。昆虫寄生菌は本種の主な天敵とされていなかったが、2018年7月から11月にかけて三重県津市安濃町野口のアカメガシワ林床の一区画内(およそ6 m×15 m)において、オオホシカメムシ成虫、ヒメホシカメムシ成虫、およびそのいずれかの幼虫の糸状菌感染によると思われる死体がそれぞれ75, 8, および7体発見された。死体の多くは発見時または追培養後に表面に緑色または白色の菌体の叢生が認められた。その菌体部分から分離された83菌株は形態的特徴から、80株がMetarhizium anisopliae sensu lato (s.l.)に、3株がBeauveria bassiana s.l.に同定された。M. anisopliae s.l.の分離株は全てほぼ同一の形態的特徴を有し、分生子塊はSabouraoud Dextrose寒天培地上では黄褐色、Potato Dextrose寒天培地上では暗緑色であった。同じ採集地ではゾウムシ科およびケバエ科の昆虫のM. anisopliae s.l.感染によると思われる死体がそれぞれ1および2体発見されたが、それらの分離株もほぼ同一の形態的特徴を示し、全て同一系統であることが示唆された。発表者は三重県松阪市、岡山県および福岡県でも、M. anisopliae s.l.感染により死亡したと思われるオオホシカメムシおよびヒメホシカメムシの死体を発見していたことから、この2種のカメムシは国内の広い地域でM. anisopliae s.l.に寄生されていると考えられた。今後はこれらのM. anisopliae s.l.分離株について、高宿主域タイプのMetarhizium spp.との分子系統学的関係性と寄主選好性とを明らかにする予定である。

  • 細菌感染防御反応におけるカイコセルピンの役割について

    〇#芳野大輔,@飯山和弘,@李 在萬,@日下部宜宏,@西 大海,@青木智佐

    平成31年度蚕糸・昆虫機能利用学術講演会 日本蚕糸学会第89回大会  2019.3 

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    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:東京農工大学小金井キャンパス   Country:Japan  

  • 乳化病菌Paenibacillus popilliaeが産生する殺虫性因子に関する研究

    〇#川原顕生,@飯山和弘,@浅野眞一郎,@西 大海,@青木智佐

    平成31年度蚕糸・昆虫機能利用学術講演会 日本蚕糸学会第89回大会  2019.3 

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    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:東京農工大学小金井キャンパス   Country:Japan  

  • トマト葉かび病菌のエフェクターAvr2は菌寄生菌Dicyma pulvinataのシステインプロテアーゼ活性を抑制する

    @◯須志田浩稔,@西 大海,@東佑実子,@鈴木智子,@中保一浩,@飯田祐一郎

    平成31年度植物病理学会大会  2019.3 

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    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:筑波大学   Country:Japan  

  • 菌寄生菌Dicyma pulvinataと麹菌Aspergillus oryzaeに保存されるセスキテルペンdeoxyphomenoneの生合成遺伝子クラスター

    @西 大海,@須志田浩稔,@東佑実子,@中川博之,@池田健太郎,@酒井 宏,@中保一浩,@◯飯田祐一郎

    平成31年度植物病理学会大会  2019.3 

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    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:筑波大学   Country:Japan  

  • オオホシカメムシに流行病を起こすメタリジウム菌

    @◯西 大海

    第63回日本応用動物昆虫学会大会  2019.3 

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    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:筑波大学   Country:Japan  

  • Identification and functional analysis of two novel Cry proteins from Paenibacillus popillaie ATCC14706 International conference

    Akio Kawahara, Kazuhiro Iiyama, Shin-ichiro Asano, Oumi Nishi, Chisa Yasunaga-Aoki

    The 2019 International Congress on Invertebrate Pathology and Microbial Control & 52nd Annual Meeting of the Society for Invertebrate Pathology & 17th Meeting of the IOBC‐WPRS Working Group “Microbial and Nematode Control of Invertebrate Pests”  2019.7 

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    Language:English   Presentation type:Oral presentation (general)  

  • Metarhizium 属糸状菌の遺伝子型分類群ごとの土壌中の生菌密度(P34)

    西大海

    日本菌学会第64回大会  2020.6 

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    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  • カイコ病原性微胞子虫Nosema bombycisゲノム上に見い出されたレクチン様遺伝子クラスターについて

    和佐野直也, 蔣師東, 西 大海, 青木智佐

    令和3年度日本蚕糸学会第91回蚕糸・昆虫機能利用学術講演会  2021.3 

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    Country:Japan  

  • Nosema bombycis のゲノム上に見い出された酵母胞子形成タンパク質 RMD1 ホモログについて

    蔣師東, 和佐野直也, 西 大海, 青木智佐

    令和3年度日本蚕糸学会第91回蚕糸・昆虫機能利用学術講演会  2021.3 

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    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  • 昆虫寄生菌製剤由来の菌株のナミハダニに対する病原力

    #中原波留加, @西 大海, @青木智佐

    第65回日本応用動物昆虫学会大会  2021.3 

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    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:島根大学(オンライン)   Country:Japan  

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Professional Memberships

  • 日本応用動物昆虫学会

  • 日本菌学会

  • 昆虫病理研究会

Committee Memberships

  • 昆虫病理研究会   Organizer   Domestic

    2021.10 - 2023.9   

Academic Activities

  • 座長

    日本菌学会第67回大会  ( Japan ) 2023.5

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    Type:Competition, symposium, etc. 

  • Screening of academic papers

    Role(s): Peer review

    2023

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    Type:Peer review 

    Number of peer-reviewed articles in foreign language journals:3

    Number of peer-reviewed articles in Japanese journals:1

  • Screening of academic papers

    Role(s): Peer review

    2022

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    Type:Peer review 

    Number of peer-reviewed articles in foreign language journals:5

    Number of peer-reviewed articles in Japanese journals:1

  • Journal of Applied Entomology and Zoology International contribution

    Role(s): Peer review

    2020.1 - 2024.1

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    Type:Academic society, research group, etc. 

  • Screening of academic papers

    Role(s): Peer review

    2020

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    Type:Peer review 

    Number of peer-reviewed articles in foreign language journals:6

    Number of peer-reviewed articles in Japanese journals:1

  • Mycoscience International contribution

    2019.6 - Present

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    Type:Academic society, research group, etc. 

  • 座長、学生発表の審査

    日本菌学会第63回大会  ( Japan ) 2019.5

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    Type:Competition, symposium, etc. 

  • Screening of academic papers

    Role(s): Peer review

    2019

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    Type:Peer review 

    Number of peer-reviewed articles in foreign language journals:10

  • Screening of academic papers

    Role(s): Peer review

    2018

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    Type:Peer review 

    Number of peer-reviewed articles in foreign language journals:3

  • Screening of academic papers

    Role(s): Peer review

    2017

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    Type:Peer review 

    Number of peer-reviewed articles in foreign language journals:3

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Research Projects

  • 南西諸島におけるMetarhizium属菌類の種多様性の解明と内生細菌探索のための抗生物質フリーな菌株ライブラリの作成

    2023.4 - 2024.3

    Joint research

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    Authorship:Collaborating Investigator(s) (not designated on Grant-in-Aid)  Grant type:Other funds from industry-academia collaboration

  • Basic study for modification of host selectivity through gene disruption in entomopathogenic fungi with broad host range

    Grant number:23K05261  2023 - 2025

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

    西 大海

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    Authorship:Principal investigator  Grant type:Scientific research funding

    冬虫夏草類に所属する、殺虫剤としての利用が多い広宿主域の昆虫寄生菌は、害虫だけでなくその捕食性天敵や花粉媒介者などの益虫類にも感染する場合がある。本研究では、広宿主域の昆虫寄生菌について、特定の遺伝子を破壊することで益虫類に感染しにくくなるように選択性を改良することを目指し、寄生性関連遺伝子の選択性の解明に取り組む。また、従来法で遺伝子を破壊すると外来DNAが導入された遺伝子組換え体となるため、その遺伝子破壊株を殺虫剤として利用する際には、遺伝子汚染などの生物多様性リスクが生じる。本研究ではそのリスクの最小化のため、ゲノム編集を利用した外来DNAを導入しない遺伝子破壊法の確立にも取り組む。

    CiNii Research

  • 昆虫寄生菌の根圏および非根圏における定着状態のin situ観察による解明

    2020

    令和2(2020)年度 農学研究院若手教員支援事業 チャレンジ研究支援(I型)

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    Authorship:Principal investigator  Grant type:On-campus funds, funds, etc.

  • 昆虫寄生菌の土壌中における生残と感染に対する物理的ストレスと土壌静菌作用の影響

    Grant number:19K23677  2019 - 2020

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Research Activity start-up

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    Authorship:Principal investigator  Grant type:Scientific research funding

  • 昆虫寄生菌の土壌中における定着能力に関する研究

    2019

    平成31(2019)年度 農学研究院若手教員支援事業 チャレンジ研究支援(I型)

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    Authorship:Principal investigator  Grant type:On-campus funds, funds, etc.

  • 広宿主域の昆虫寄生菌における益虫選択的病原力遺伝子の探索

    2019

    2019年度QRプログラム わかばチャレンジ【若手研究(挑戦的研究)】

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    Authorship:Principal investigator  Grant type:On-campus funds, funds, etc.

  • Metarhizium属糸状菌における寄生能の多面性とその進化的背景の解明

    Grant number:14J09097  2014 - 2016

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for JSPS Fellows

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    Authorship:Principal investigator  Grant type:Scientific research funding

  • 昆虫病原性糸状菌の系統解析と生態特性

    Grant number:11J00275  2011 - 2013

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for JSPS Fellows

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    Authorship:Principal investigator  Grant type:Scientific research funding

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Class subject

  • 農業生物科学演習第二

    2023.10 - 2024.3   Second semester

  • 農業生物科学演習第一

    2023.10 - 2024.3   Second semester

  • 自然科学総合実験

    2023.10 - 2023.12   Fall quarter

  • 昆虫病理学特論

    2023.10 - 2023.12   Fall quarter

  • 農業生物科学特別研究第二

    2023.4 - 2024.3   Full year

  • 農業生物科学特別研究第一

    2023.4 - 2024.3   Full year

  • Introduction to Bioresource and Bioenvironment 2 ・生物資源環境科学入門2

    2023.4 - 2023.6   Spring quarter

  • 農業生物科学演習第二

    2022.10 - 2023.3   Second semester

  • 農業生物科学演習第一

    2022.10 - 2023.3   Second semester

  • 昆虫病理学特論

    2022.10 - 2022.12   Fall quarter

  • 農業生物科学特別研究第二

    2022.4 - 2023.3   Full year

  • 農業生物科学特別研究第一

    2022.4 - 2023.3   Full year

  • Introduction to Bioresource and Bioenvironment 2 ・生物資源環境科学入門2

    2022.4 - 2022.6   Spring quarter

  • 農業生物科学演習第一

    2021.10 - 2022.3   Second semester

  • 農業生物科学演習第二

    2021.10 - 2022.3   Second semester

  • 昆虫病理学特論

    2021.10 - 2021.12   Fall quarter

  • 農業生物科学特別研究第二

    2021.4 - 2022.3   Full year

  • 農業生物科学特別研究第一

    2021.4 - 2022.3   Full year

  • 自然科学総合実験

    2021.4 - 2021.6   Spring quarter

  • Introduction to Bioresource and Bioenvironment 2 ・生物資源環境科学入門2

    2021.4 - 2021.6   Spring quarter

  • 農業生物科学演習第二

    2020.10 - 2021.3   Second semester

  • 農業生物科学演習第一

    2020.10 - 2021.3   Second semester

  • 昆虫病理学特論

    2020.10 - 2020.12   Fall quarter

  • 農業生物科学特別研究第二

    2020.4 - 2021.3   Full year

  • 農業生物科学特別研究第一

    2020.4 - 2021.3   Full year

  • Introduction to Bioresource and Bioenvironment 2 ・生物資源環境科学入門2

    2020.4 - 2020.6   Spring quarter

  • 農業生物科学演習第二

    2019.10 - 2020.3   Second semester

  • 昆虫病理学特論

    2019.10 - 2019.12   Fall quarter

  • 農業生物科学特別研究第一

    2019.4 - 2020.3   Full year

  • 農業生物科学特別研究第二

    2019.4 - 2020.3   Full year

  • Introduction to Bioresource and Bioenvironment II ・農学入門II

    2019.4 - 2019.6   Spring quarter

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FD Participation

  • 2021.7   Role:Participation   Title:農学研究院FD「科研費を獲りにいこう! 科研費獲得の技術と工夫」

    Organizer:[Undergraduate school/graduate school/graduate faculty]

  • 2021.3   Role:Participation   Title:大学院学際教育の質向上に関するFD:2018-2019年度NEEP成果をふまえて

    Organizer:[Undergraduate school/graduate school/graduate faculty]

  • 2020.12   Role:Participation   Title:大学の研究評価の現状と農学研究院の「部局独自の評価基準」案における業績分析

    Organizer:[Undergraduate school/graduate school/graduate faculty]

  • 2020.9   Role:Participation   Title:科研費を獲りにいこう! 勝ち抜く気合と技術

    Organizer:[Undergraduate school/graduate school/graduate faculty]

  • 2020.6   Role:Participation   Title:九州大学はどのような教育を目指していくのか-教学マネジメント枠組みに則した教員活動評価に向けてー

    Organizer:[Undergraduate school/graduate school/graduate faculty]

Social Activities

  • オープンキャンパス/生物的防除研究施設天敵微生物学部門の研究内容について来学した一般者に紹介した。

    九州大学  九州大学伊都キャンパスウエスト5号館  2023.8

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    Audience:General, Scientific, Company, Civic organization, Governmental agency

    Type:Lecture

  • オープンキャンパス/生物的防除研究施設天敵微生物学部門の研究内容について動画で一般者に紹介した。

    九州大学  オンライン  2022.8

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    Audience:General, Scientific, Company, Civic organization, Governmental agency

    Type:Lecture

  • オープンキャンパス/生物的防除研究施設天敵微生物学部門の研究内容について動画で一般者に紹介した。

    九州大学  オンライン  2021.8

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    Audience:General, Scientific, Company, Civic organization, Governmental agency

    Type:Lecture

  • オープンキャンパス/生物的防除研究施設天敵微生物学部門の研究内容について動画で一般者に紹介した。

    九州大学  九州大学(オンライン)  2020.8

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    Audience:General, Scientific, Company, Civic organization, Governmental agency

    Type:Lecture

  • オープンキャンパス/生物的防除研究施設天敵微生物学部門の研究内容について、昆虫寄生菌の標本などを展示して一般者に紹介した。

    九州大学  九州大学ウエスト5号館  2019.8

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    Audience:General, Scientific, Company, Civic organization, Governmental agency

    Type:Lecture

Acceptance of Foreign Researchers, etc.

  • University of Texas Rio Grande Valley

    Acceptance period: 2022.9 - 2022.10   (Period):1 month or more

    Nationality:United States

    Business entity:Japan Society for the Promotion of Science