Faculty Profiles - TSUTSUMI YUJI

Information

写真a

TSUTSUMI YUJI

Organization

Faculty of Agriculture Department of Agro-environmental Sciences Professor
Institute of Tropical Agriculture （Concurrent）

Kyushu University Low Temperature Center （Concurrent）
School of Agriculture Department of Bioresource and Bioenvironment（Concurrent）
Graduate School of Bioresource and Bioenvironmental Sciences Department of Agro-environmental Sciences（Concurrent）

Contact information

Tel

0928024664

Profile

(1) Studies on lignin biosynthesis in woody plant cell walls Lignin is the second abundant biomass on the earth. Biosynthetic mechanisms of lignin in woody plant are investigated from biochemical and molecular biological aspects. (2)Bioremediation of organic pollutants by white-rot fugi. Biodegradation of organic pollutants such as dioxins, PCBs, and other chlorinated compounds are investigated. The enzymes and reaction mechanisms involved in the degradation reaction are studied. (3)Phyto-remediation Phyto-remediation is an approach for remediating the polluted environments by using plants. Study on the potential of phyto-remediation using woody plants has been started.

Homepage

http://ffpsc.agr.kyushu-u.ac.jp/chem/

External link

Degree

PhD.

Research History

神崎製紙（株）（現王子製紙（株））1998年4月〜1999年3月

神崎製紙（株）（現王子製紙（株））1998年4月〜1999年3月
静岡大学　農学部　助教授　1996年3月〜2002年2月静岡大学　農学部　助手　　1994年1月〜1996年2月

Research Interests・Research Keywords

Research theme： Mechanism of lignin degradation promoted by symbiosis between white rot fungi and bacteria

Keyword： white rot fungi, bacteria, symbiosis, lignin degradation

Research period： 2023.4 - 2026.3
Research theme： Development of functional lignin-cellulose composite film

Keyword： Lignin. All-round peroxidase, Composite film

Research period： 2020.4 - 2026.3
Research theme： Degradation and inactivation of prion by white-rot fungi and enzymes

Keyword： white-rot fungi, enzyme, prion, inactivation

Research period： 2005.4 - 2011.5
Research theme： Utilization of woody-biomass for sustainable energy

Keyword： woody-biomass, sustainable energy

Research period： 2005.4
Research theme： Biodegradation of recalcitrant organic pollutants by white-rot fungi.

Keyword： biodegradation, degradation, white-rot fungi.

Research period： 1996.1
Research theme： Mechanisms on the formation of woody plant cell walls -lignin biosynthesis-.

Keyword： lignin, biosynthesis, cell wall

Research period： 1993.4

Awards

日本木材学会賞

2010.1 日本木材学会 “Peroxidase and 4-coumarate:CoA ligase related to lignin biosynthesis”

Papers

The Populus alba cationic cell‐wall‐bound peroxidase (CWPO‐C) regulates plant growth, lignin content and composition in poplar Reviewed International journal

#Diego Alonso Yoshikay‐Benitez, @Kaori Ohira, @Kasturi Banerjee, Koki Fujita, @Jun Shigeto, Yuji Tsutsumi

Journal of Wood Science 12 - 12 2023.3

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DOI： 10.1186/s10086-023-02086-1
Populus alba cationic cell-wall-bound peroxidase (CWPO-C) regulates the plant growth and affects auxin concentration in Arabidopsis thaliana Reviewed International journal

#Diego Alonso Yoshikay‐Benitez, #Yusuke Yokoyama, #Kaori Ohira, @Koki Fujita, Azusa Tomie, Yoshio Kijidani, Jun Shigeto, @Yuji Tsutsumi

Physiology and Molecular Biology of Plants 2022.10

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The poplar cationic cell-wall-bound peroxidase (CWPO-C) mediates the oxidative polymerization of lignin precursors, especially sinapyl alcohols, and high molecular weight compounds that cannot be oxidized by other plant peroxidases, including horseradish peroxidase C. Therefore, CWPO-C is believed to be a lignification-specific peroxi- dase, but direct evidence of its function is lacking. Thus, the CWPO-C expression pattern in Arabidopsis thaliana (Arabi- dopsis) was determined using the β-glucuronidase gene as a reporter. Our data indicated that CWPO-C was expressed in young organs, including the meristem, leaf, root, flower, and young xylem in the upper part of the stem. Compared with the wild-type control, transgenic Arabidopsis plants overexpressing CWPO-C had shorter stems. Approximately 60% of the plants in the transgenic line with the highest CWPO-C content had curled stems. These results indicate that CWPO-C plays a role in cell elongation. When plants were placed horizontally, induced CWPO-C expression was detected in the curved part of the stem during the gravitropic response. The stem curvature associated with gravitropism is controlled by auxin localization. The time needed for Arabi- dopsis plants overexpressing CWPO-C placed horizontally to bend by 90° was almost double the time required for the similarly treated wild-type controls. Moreover, the auxin content was significantly lower in the CWPO-C-overex- pressing plants than in the wild-type plants. These results strongly suggest that CWPO-C has pleiotropic effects on plant growth and indole-3-acetic acid (IAA) accumulation. These effects may be mediated by altered IAA concentration due to oxidation.

DOI： 10.1007/s12298-022-01241-0
The gene expression analysis of Arabidopsis thaliana ABC transporters by real-time PCR for screening monolignol-transporter candidates Reviewed International journal

Manami Takeuchi, Watanabe Atsushi, Miho Tamura, Yuji Tsutsumi

Journal of Wood Science 64 477 - 484 2018.10

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The transport of monolignols from the cytosol to the cell wall is essential for lignin synthesis. The ATP-binding cassette (ABC) transporters may be involved in the transport of lignin precursors. ABC transporter genes subjected to expression analysis were chosen based on two criteria for screening candidate transporter genes related to lignification. The expression levels of 15 target genes in five plant organs were analyzed by real-time PCR. Five transporter genes (ABCG29, ABCG30, ABCG33, ABCG34, and ABCG37), which were simultaneously expressed with the reference genes, were selected as candi- dates. The candidate gene expression levels in root tissues of T-DNA insertion mutants were determined by semi-quantitative reverse transcription PCR. ABCG30 was more highly expressed in the abcg34 mutant than in the wild-type plants, while the expression of ABCG34 was twofold higher in the abcg30 mutant plants than in the wild-type plants. Thus, the expression of ABCG30 and ABCG34 may affect each other. There was no significant change in lignin content and composition in the single-gene knockout mutants of the candidate transporter genes, which suggested that each candidate gene did not solely contribute to lignin synthesis.

DOI： 10.1007/s10086-018-1733-9
Expression analysis of transporter genes for screening candidate monolignol transporters using Arabidopsis thaliana cell suspensions during tracheary element differentiation Reviewed International journal

Manami Takeuchi, Takahiro Kegasa, Watanabe Atsushi, Miho Tamura, Yuji Tsutsumi

Journal of Plant Research 131 297 - 305 2018.3

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The mechanism of monolignol transportation from the cytosol to the apoplast is still unclear despite being an essential step of ligni cation. Recently, ATP-binding cas- sette (ABC) transporters were suggested to be involved in monolignol transport. However, there are no reliable clues to the transporters of the major lignin monomers coniferyl and synapyl alcohol. In this study, the ligni cation progress of Arabidopsis cultured cells during tracheary element dif- ferentiation was monitored. The expression of selected trans- porter genes, as well as ligni cation and cell-wall formation related genes as references, in di erentiating cultured cell samples harvested at 2-day intervals was analyzed by real- time PCR and the data were statistically processed. The cell wall formation transcription factor MYB46, programmed- cell death related gene XCP1 and lignin polymerization peroxidase AtPrx25 were classi ed into the same cluster. Furthermore, the cluster closest to the abovementioned cluster contained the lignin synthesis transcription factor.

DOI： 10.1007/s10265-017-0979-4
Generation of lignin polymer models via dehydrogenative polymerization of coniferyl alcohol and syringyl alcohol via several plant peroxidases involved in lignification and analysis of the resulting DHPs by MALDI-TOF analysis Reviewed International journal

Jun Shigeto, Hiroki Honjo, Koki Fujita and Yuji Tsutsumi

Holzforschung 72 ( 4 ) 267 - 274 2017.12

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The mechanism of lignin dehydrogenative poly- merization (DHP), made by means of horseradish peroxi- dase (HRP), was studied in comparison with other plant peroxidases. Interestingly, HRP is efficient for guaiacyl type polymer formation (G-DHPs), but is not efficient in the case of syringyl type DHPs (S-DHPs). It was previously demonstrated that lignification-related Arabidopsis thali- ana peroxidases, AtPrx2, AtPrx25 and AtPrx71, and cati- onic cell-wall-bound peroxidase (CWPO-C) from Populus alba are successful to oxidize syringyl- and guaiacyl-type monomers and larger lignin-like molecules. This is the reason why in the present study the DHP formation by means of these recombinant peroxidases was tested, and all these enzymes were successful for formation of both G-DHP and S-DHP in acceptable yields. CWPO-C led to S-DHP molecular size distribution similar to that of iso- lated lignins.

DOI： 10.1515/hf-2017-0125
Enzymatic activities for lignin monomer intermediates highlight the biosynthetic pathway of syringyl monomers in Robinia pseudoacacia Reviewed International journal

Jun Shigeto, Yukie Ueda, Shinya Sasaki, Koki Fujita, Yuji Tsutsumi

Journal of Plant Research 130 ( 1 ) 203 - 210 2017.1

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Most of the known 4-coumarate:coenzyme A ligase (4CL) isoforms lack CoA-ligation activity for sinapic acid. Therefore, there is some doubt as to whether sinapic acid contributes to sinapyl alcohol biosynthesis. In this study, we characterized the enzyme activity of a protein mixture extracted from the developing xylem of Robinia pseudoacacia. The crude protein mixture contained at least two 4CLs with sinapic acid 4-CoA ligation activity. The crude enzyme preparation displayed negligible sinapaldehyde dehydrogenase activity, but showed ferulic acid 5-hydroxylation activity and 5-hydroxyferulic acid O-methyltransferase activity; these activities were retained in the presence of competitive substrates (coniferaldehyde and 5-hydroxyconiferaldehyde, respectively). 5-Hydroxyferulic acid and sinapic acid accumulated in the developing xylem of R. pseudoacacia, suggesting, in part at least, sinapic acid is a sinapyl alcohol precursor in this species.

DOI： 10.1007/s10265-016-0882-4
Phosphoric acid pretreatment of Achyranthes aspera and Sida acuta weed biomass to improve enzymatic hydrolysis Reviewed International journal

Premjet Siripong, Premjet Duangporn, Eri Takata, Yuji Tsutsumi

Bioresource Technology 203 303 - 308 2016.3

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Achyranthes aspera and Sida acuta, two types of weed biomass are abundant and waste in Thailand. We focus on them as novel feedstock for bio-ethanol production because they contain high-cellulose content (45.9% and 46.9%, respectively) and unutilized material. Phosphoric acid (70%, 75%, and 80%) was employed for the pretreatment to improve by enzymatic hydrolysis. The pretreatment process removed most of the xylan and a part of the lignin from the weeds, while most of the glucan remained. The cellulose conversion to glucose was greater for pretreated A. aspera (86.2 ± 0.3%) than that of the pretreated S. acuta (82.2 ± 1.1%). Thus, the removal of hemicellulose significantly affected the efficiency of the enzymatic hydrolysis. The scanning electron microscopy images showed the exposed fibrous cellulose on the cell wall surface, and this substantial change of the surface structure contributed to improving the enzyme accessibility.

DOI： 10.1016/j.biortech.2015.12.037
Simultaneously disrupting AtPrx2, AtPrx25 and AtPrx71 alters lignin content and structure in Arabidopsis stem Reviewed International journal

Jun Shigeto, Itoh Yoshitaka, Hrao Sakie, Ohhira Kaori, Koki Fujita, Yuji Tsutsumi

JOURNAL OF INTEGRATIVE PLANT BIOLOGY 57 ( 4 ) 349 - 356 2015.4

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Lignins are aromatic heteropolymers that arise from oxidative coupling of lignin precursors, including lignin monomers (p-coumaryl, coniferyl, and sinapyl alcohols), oligomers, and polymers. Whereas plant peroxidases have been shown to catalyze oxidative coupling of monolignols, the oxidation activity of well-studied plant peroxidases, such as horseradish peroxidase C (HRP-C) and AtPrx53, are quite low for sinapyl alcohol. This characteristic difference has led to controversy regarding the oxidation mechanism of sinapyl alcohol and lignin oligomers and polymers by plant peroxidases. The present study explored the oxidation activities of three plant peroxidases, AtPrx2, AtPrx25, and AtPrx71, which have been already shown to be involved in lignification in the Arabidopsis stem. Recombinant proteins of these peroxidases (rAtPrxs) were produced in Escherichia coli as inclusion bodies and successfully refolded to yield their active forms. rAtPrx2, rAtPrx25, and rAtPrx71 were found to oxidize two syringyl compounds (2,6-dimethoxyphenol and syringaldazine), which were employed here as model monolignol compounds, with higher specific activities than HRP-C and rAtPrx53. Interestingly, rAtPrx2 and rAtPrx71 oxidized syringyl compounds more efficiently than guaiacol. Moreover, assays with ferrocytochrome c as a substrate showed that AtPrx2, AtPrx25, and AtPrx71 possessed the ability to oxidize large molecules. This characteristic may originate in a protein radical. These results suggest that the plant peroxidases responsible for lignin polymerization are able to directly oxidize all lignin precursors.

DOI： 10.1111/jipb.12334
Catalytic Profile of Arabidopsis Peroxidases, AtPrx-2, 25 and 71, Contributing to Stem Lignification Reviewed International journal

Jun Shigeto, Mariko Nagano, Koki Fujita, Yuji Tsutsumi

PLoS ONE 9 ( 8 ) e105332 2014.8

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Lignins are aromatic heteropolymers that arise from oxidative coupling of lignin precursors, including lignin monomers (p-coumaryl, coniferyl, and sinapyl alcohols), oligomers, and polymers. Whereas plant peroxidases have been shown to catalyze oxidative coupling of monolignols, the oxidation activity of well-studied plant peroxidases, such as horseradish peroxidase C (HRP-C) and AtPrx53, are quite low for sinapyl alcohol. This characteristic difference has led to controversy regarding the oxidation mechanism of sinapyl alcohol and lignin oligomers and polymers by plant peroxidases. The present study explored the oxidation activities of three plant peroxidases, AtPrx2, AtPrx25, and AtPrx71, which have been already shown to be involved in lignification in the Arabidopsis stem. Recombinant proteins of these peroxidases (rAtPrxs) were produced in Escherichia coli as inclusion bodies and successfully refolded to yield their active forms. rAtPrx2, rAtPrx25, and rAtPrx71 were found to oxidize two syringyl compounds (2,6-dimethoxyphenol and syringaldazine), which were employed here as model monolignol compounds, with higher specific activities than HRP-C and rAtPrx53. Interestingly, rAtPrx2 and rAtPrx71 oxidized syringyl compounds more efficiently than guaiacol. Moreover, assays with ferrocytochrome c as a substrate showed that AtPrx2, AtPrx25, and AtPrx71 possessed the ability to oxidize large molecules. This characteristic may originate in a protein radical. These results suggest that the plant peroxidases responsible for lignin polymerization are able to directly oxidize all lignin precursors.

DOI： 10.1371/journal.pone.0105332
Production of xylitol and tetrahydrofurfuryl alcohol from xylan in napier grass by a hydrothermal process with phosphorus oxoacids followed by aqueous phase hydrogenation Reviewed International journal

Eri Takata, Tatsushi Tsuruoka, Ken Tsutsumi, Yuji Tsutsumi, Kenji Tabata

Bioresource Technology 167 74 - 80 2014.6

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The production of xylitol and tetrahydrofurfuryl alcohol (THFA) from napier grass was studied using two
steps: a hydrothermal process with phosphorus oxoacids followed by aqueous phase hydrogenation with
Pd/C. Xylose obtained from the napier grass by the hydrothermal treatment with 3.0 wt% phosphorous
acid was subsequently converted into xylitol at 51.6% yield of the xylan in napier grass by hydrogenation
with 5.0 wt% Pd/C. The furfural produced from napier grass with a 3.0 wt% phosphoric acid treatment was
also directly subjected to the hydrogenation as a hydrolysate to yield 41.4% THFA based on the xylan in
napier grass. The yields of xylitol and THFA obtained by hydrogenation using the napier grass hydrolysate
containing xylose or furfural were almost the same as those of hydrogenation using commercial materi-
als. To our knowledge, this is the first report on the production of THFA in high yield by hydrogenation
directly from biomass hydrolysate.

DOI： 10.1016/j.biortech.2014.05.112
Production of monosaccharides from napier grass by hydrothermal process with phosphoric acid Reviewed International journal

Eri Takata, Ken Tsutsumi, Yuji Tsutsumi, Kenji Tabata

Bioresource Technology 143 53 - 58 2013.9

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DOI： 10.1016/j.biortech.2013.05.112
Putative Cationic Cell-Wall-Bound Peroxidase Homologues in Arabidopsis, AtPrx2, AtPrx25, and AtPrx71, Are Involved in Lignification Reviewed International journal

Jun Shigeto, Yuko Kiyonaga, Koki Fujita, RYUICHIRO KONDO, Yuji Tsutsumi

J. Agric. Food Chem. 61 ( 16 ) 3781 - 3788 2013.4

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The final step of lignin biosynthesis, which is catalyzed by a plant peroxidase, is the oxidative coupling of the
monolignols to growing lignin polymers. Cationic cell-wall-bound peroxidase (CWPO-C) from poplar callus is a unique enzyme
that has oxidative activity for both monolignols and synthetic lignin polymers. This study shows that putative CWPO-C
homologues in Arabidopsis, AtPrx2, AtPrx25, and AtPrx71, are involved in lignin biosynthesis. Analysis of stem tissue using the
acetyl bromide method and derivatization followed by the reductive cleavage method revealed a significant decrease in the total
lignin content of ATPRX2 and ATPRX25 deficient mutants and altered lignin structures in ATPRX2, ATPRX25, and ATPRX71
deficient mutants. Among Arabidopsis peroxidases, AtPrx2 and AtPrx25 conserve a tyrosine residue on the protein surface, and
this tyrosine may act as a substrate oxidation site as in the case of CWPO-C. AtPrx71 has the highest amino acid identity with
CWPO-C. The results suggest a role for CWPO-C and CWPO-C-like peroxidases in the lignification of vascular plant cell walls.

DOI： 10.1021/jf400426g
Identification of Tyr74 and Tyr177 as substrate oxidation sites in cationic cell wall-bound peroxidase from Populus alba L. International journal

Shigeto Jun;Itoh Yoshitaka;Tsutsumi Yuji;et al.

FEBS JOURNAL 279 ( 2 ) 2012.1

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DOI： 10.1111/j.1742-4658.2011.08429x
Saline-dependent regulation of manganese peroxidase genes in the hypersaline-tolerant white rot fungus Phlebia sp. MG-60 Reviewed International journal

I. Kamei, C. Daikoku, Y. Tsutsumi, R. Kondo

Applied and Environmental Microbiology 2008.4

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Transcriptional and translational analyses of poplar anionic peroxidase isoenzymes. J. Wood. Sci., Reviewed International journal

Sasaki S, Shimizu S, Wariishi H, Tsutsumi Y, Kondo R

J. Wood. Sci. 2007.12

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Role of Tyr residues on the protein surface of cationic cell-wall-peroxidase (CWPO-C) from poplar: Potentially unique oxidation sites for oxidative polymerization of lignin. Reviewed International journal

Shinya Sasaki, Daisuke Nonaka, Hiroyuki Wariishi, Yuji Tsutsumi, Ryuichiro Kondo

Phytochemistry 2007.3

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Isolation and characterization of a novel bacterium, Sphingomonas bisphenolicum strain AO1, that degrades bisphenol A Reviewed International journal

K. Oshiman, Y. Tsutsum, T. Nishida, Y. Matsumura

Biodegradation 2007.2

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The cationic cell-wall-peroxidase having oxidation ability for polymeric substrate participates in the late stage of lignification of Populus alba L Reviewed International journal

S. Sasaki, K. Baba, T. Nishida, Y. Tsutsumi, R. Kondo

Plant Mol. Biol. 2006.12

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4-Coumarate:coenzyme A ligase in black locust (Robinia pseudoacacia) catalyses the conversion of sinapate to sinapoyl-CoA. Reviewed International journal

K. Hamada, T. Nishida, K. Yamauchi, K. Fukushima, R. Kondo, Y. Tsutsumi

Journal of Plant Research 117 ( 4 ) 303 - 310 2004.8

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DOI： 10.1007/s10265-004-0159-1
Lignin dehydrogenative polymerization mechanism: a poplar cell wall peroxidase directly oxidizes polymer lignin and produces in vitro dehydrogenative polymer rich in beta-O-4 linkage. Reviewed International journal

S. Sasaki, T. Nishida, Y. Tsutsumi, R. Kondo.

FEBS Letters 562 ( 1-3 ) 197 - 201 2004.4

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DOI： 10.1016/S0014-5793(04)00224-8
Treatment of poplar callus with ferulic and sinapic acids II: Effect on related monolignol biosynthetic pathway enzyme activities. Reviewed International journal

K Hamada, Y. Tsutsumi, T. Nishida

J. Wood Sci. 49 ( 4 ) 366 - 370 2003.8

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DOI： 10.1007/s10086-002-0475-9
Treatment of poplar callus with ferulic and sinapic acids I: Incorporation and enhancement of lignin biosynthesis. Reviewed International journal

K Hamada, Y. Tsutsumi, K. Yamauchi, K. Fukushima, T. Nishida

J. Wood Sci. 49 ( 4 ) 333 - 338 2003.8

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DOI： 10.1007/s10086-002-0477-7
Multiform biosynthetic pathway of syringyl lignin in angiosperms Reviewed International journal

K.Yamauchi, S. Yasuda, K. Hamada, Y. Tsutsumi, K. Fukushima

Planta 216 ( 3 ) 496 - 501 2003.1

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DOI： 10.1007/s00425-002-0865-7
Sinapyl alcohol-specific peroxidase isoenzyme catalyzes the formation of the dehydrogenative polymer from sinapyl alcohol. Reviewed International journal

W. Aoyama, S. Sasaki, S. Matsumura, T. Mitsunaga, H. Hirai, Y. Tsutsumi, T. Nishida

J. Wood Sci. 48 ( 6 ) 497 - 504 2002.12

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DOI： 10.1007/BF00766646
Removal of estrogenic activities of bisphenol a and nonylphenol by oxidative enzymes from lignin-degrading basidiomycetes. Reviewed International journal

Tsutsumi Y., Haneda T., Nishida T.

Chemosphere 2001.1

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Lignification and peroxidase in tension wood of Eucalyptus viminalis seedlings. Reviewed International journal

Aoyama W., Matsumura A., Tsutsumi Y., Nishida T

J. Wood Sci. 2001.1

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Subcritical Water Hydrolysis Effectively Reduces the In Vitro Seeding Activity of PrPSc but Fails to Inactivate the Infectivity of Bovine Spongiform Encephalopathy Prions Reviewed International journal

Yuichi Murayama, Miyako Yoshioka, Hiroyuki Okada, Eri Takata, Kentaro Masujin, Yoshifumi Iwamaru, Noriko Shimozaki, Tomoaki Yamamura, Takashi Yokoyama, Shirou Mohri, Yuji Tsutsumi

PLoS One 10 ( 12 ) e0144761 2015.12

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The global outbreak of bovine spongiform encephalopathy (BSE) has been attributed to the recycling of contaminated meat and bone meals (MBMs) as feed supplements. The use of MBMs has been prohibited in many countries; however, the development of a method for inactivating BSE prions could enable the efficient and safe use of these products as an organic resource. Subcritical water (SCW), which is water heated under pressure to maintain a liquid state at temperatures below the critical temperature (374°C), exhibits strong hydrolytic activity against organic compounds. In this study, we examined the residual in vitro seeding activity of protease-resistant prion protein (PrPSc) and the infectivity of BSE prions after SCW treatments. Spinal cord homogenates prepared from BSE-infected cows were treated with SCW at 230–280°C for 5–7.5 min and used to intracerebrally inoculate transgenic mice overexpressing bovine prion protein. Serial protein misfolding cyclic amplification (sPMCA) analysis detected no PrPSc in the SCW-treated homogenates, and the mice treated with these samples survived for more than 700 days without any signs of disease. However, sPMCA analyses detected PrPSc accumulation in the brains of all inoculated mice. Furthermore, secondary passage mice, which inoculated with brain homogenates derived from a western blotting (WB)-positive primary passage mouse, died after an average of 240 days, similar to mice inoculated with untreated BSE-infected spinal cord homogenates. The PrPSc accumulation and vacuolation typically observed in the brains of BSE-infected mice were confirmed in these secondary passage mice, suggesting that the BSE prions maintained their infectivity after SCW treatment. One late-onset case, as well as asymptomatic but sPMCA-positive cases, were also recognized in secondary passage mice inoculated with brain homogenates from WB-negative but sPMCA-positive primary passage mice. These results indicated that SCW-mediated hydrolysis was insufficient to eliminate the infectivity of BSE prions under the conditions tested.

DOI： 10.1371/journal.pone.0144761
Stereo-selective oxidations of terpinolene by cytochrome P450 monooxygenases in the microsomal fraction of Cupressus lusitanica cultured cells Reviewed International journal

Takako Harada, Koki Fujita, Jun Shigeto, Yuji Tsutsumi

Journal of Wood Science 9 ( 8 ) e105332 - e105332 2014.8

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DOI： 10.1371/journal.pone.0105332
A novel synthetic pathway for tropolone ring formation via the olefin monoterpene intermediate terpinolene in cultured Cupressus lusitanica cells Reviewed International journal

Koki Fujita, Yasufumi Bunyu, Ken-ichi Kuroda, Tatsuya Ashitani, Jun Shigeto, Yuji Tsutsumi

Journal of Plant Physiology 171 ( 8 ) 610 - 614 2014.3

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DOI： 10.1016/j.jplph.2013.12.016
A rapid in vitro bioassay system for testing resistance factors of pine trees to Bursaphelenchus xylophilus Reviewed International journal

Zhang Fujia, Yasuhiro Mori, Yuji Tsutsumi, RYUICHIRO KONDO

Nematology 15 ( 6 ) 665 - 670 2013.6

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DOI： 10.1163/15685411-00002709
Metabolites from Resistant and Susceptible Pinus thunbergii after Inoculation with Pine Wood Nematode International journal

Zhang Fujia, Junko Kajiwara, Mineko Ohira, Yasuhiro Mori, Yuji Tsutsumi, RYUICHIRO KONDO

American Journal of Plant Science 4 ( 3 ) 512 - 518 2013.3

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DOI： 10.4236/ajps.2013.43065
Effects of combinational treatment with ethephon and indole-3-butyric acid on adventitious rooting of Pinus thunbergii cuttings

Y. Mori , F. Miyahara , Y. Tsutsumi and R. Kondo

Plant Growth Regulation 2011.3

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Relationship between resistance to pine wilt disease and the migration or proliferation of pine wood nematodes. Reviewed International journal

Y. Mori, F. Miyahara, Y. Tsutsumi, R. Kondo

European Journal of Plant Pathology 2008.4

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Role of Tyr residues on the protein surface of cationic cell-wall-peroxidase(CWPO-C) from poplar; Potentially unique oxidation sites for oxidative polymerization of lignin. Reviewed International journal

S. Sasaki, D. Nonaka, H. Wariishi, Y. Tsutsumi, R. Kondo

Phytochemistry 2008.4

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Steroid 9a-hydroxylation during testosterone degradation by resting Rhodococcus equi Cells. Reviewed International journal

Y. Kim, J. Han, S. S. Lee, K. Shimizu, Y. Tsutsumi, R. Kondo

Arch. Pharm. Chem. Life Sci. 2007.10

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クロマツの遺伝的要因を考慮した弱病原性マツノザイセンチュウの前接種による誘導抵抗性の検証.

森康浩・宮原文彦・堤祐司・近藤隆一郎

日本森林学会誌 2007.4

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Spontaneous Ultraweak Photon Emission during the Growth of the Cell Population of Cultured HeLa Cell Line International journal

J. Kim, Y. Kim, Y. J. Lee, M. Kobayashi, Y. Tsutsumi, R. Kondo, S. K. Lee and K.-S. Soh.

J. Health Sci. 2007.4

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Books

木質の形成　第３版　バイオマス科学への招待

福田　和彦、船田　良、杉山　淳司、高部　圭司、梅澤　俊明、山本浩之、堤　祐司他（Role：Joint author）

海青社 2024.3

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Language：Japanese Book type：Scholarly book
木質の形成　第2版　バイオマス科学への招待

福田　和彦、船田　良、杉山　淳司、高部　圭司、梅澤　俊明、山本浩之、堤　祐司他（Role：Joint author）

海青社 2011.10

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Language：Japanese Book type：Scholarly book
森をとりもどすために②　林木の育種

堤祐二　海老沼宏安　海田るみ　川口健太郎　近藤禎二　澤田真千子　外内尚人　西窪伸之　他

海青社 2010.10

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Language：Japanese Book type：General book, introductory book for general audience
木質の形成−バイオマス科学への招待−

（Role：Joint author）

海青社 2003.3

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Responsible for pages：p.219-230 Language：Japanese Book type：Scholarly book

Presentations

モウソウチク(Phyllostachys pubescens)稈由来リグニンの分解生成物中に含まれる化合物の生理活性

#廣島　将大，栫　拓巳，堤　祐司，清水　邦義

第70回日本木材学会大会 2020.3

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Event date： 2020.3

Language：Japanese Presentation type：Oral presentation (general)

Venue：鳥取大学 Country：Japan
万能ペルオキシダーゼ、 CWPO-Cによる植物の成長制御とオーキシン代謝

#横山　裕亮，@富家　梓，#YOSHIKAY　Diego，@雉子谷　佳男，堤　祐司

第70回日本木材学会大会 2020.3

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Event date： 2020.3

Language：Japanese Presentation type：Oral presentation (general)

Venue：鳥取大学 Country：Japan
細胞壁二次壁構造を模倣した三次元マトリックスフィルムの創製

#川口　なつみ，堤　祐司

第70回日本木材学会大会 2020.3

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Event date： 2020.3

Language：Japanese Presentation type：Oral presentation (general)

Venue：鳥取大学 Country：Japan
Cytotoxicity of lignin-derived compounds isolated from bamboo (Phyllostachys pubescence) on cancer cell line International conference

#Hiroshima Shota,Kakoi Takumi,#Hayashi Junya,Tsutsumi Yuji,Shimizu Kuniyoshi

1st International Lignin Symposium 2019.9

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Event date： 2019.9

Language：English Presentation type：Oral presentation (general)

Venue：Sapporo Country：Japan
Regulation of differentiation and growth by plant peroxidase CWPO-C International conference

#Diego A. Yoshikay,#Yusuke Yokoyama ,Jun Shigeto, Yuji Tsutsumi

1st International Lignin Symposium 2019.9

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Event date： 2019.9

Language：English Presentation type：Oral presentation (general)

Venue：Sapporo Country：Japan
植物ペルオキシダーゼ、 CWPO-Cによる成長制御作用機序の解明

#横山　裕亮，@富家　梓，#YOSHIKAY　Diego，@雉子谷　佳男，堤　祐司

第36回日本木材学会九州支部大会 2019.9

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Event date： 2019.9

Language：Japanese Presentation type：Oral presentation (general)

Venue：宮崎大学 Country：Japan
The contribution of CWPO-C to primary stage of plant growth and organogenesis International conference

#Diego Yoshikay, Jun Shigeto, #Yusuke Yokoyama, Yuji Tsutsumi

5th Symposium of Biotechnology Applied to Lignocelluloses 2018.8

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Event date： 2018.8 - 2018.9

Language：English Presentation type：Oral presentation (general)

Venue：Helsinki Country：Finland

Cationic cell-wall-bound peroxidase (CWPO-C) from poplar has been believed as a lignification-specific peroxidase, but direct evidence that demonstrates the role of CWPO-C remained unachieved. To promote better understanding about CWPO-C functions, transcriptional analysis of CWPO-C using laser microdissection, gene expression quantification and reporter gene techniques were performed. The results showed that CWPO-C expressed in the most of young tissues including xylem of upper stem, but scarcely expressed in interfascicular fiber and undifferentiated tissue such as apical meristem and cambium. Heterologous overexpression of CWPO-C in Arabidopsis inhibited plant growth and caused stem curvature. In addition, CWPO-C expressed in the outer site of the curved stem that was subjected to gravity stress. These results indicate that CWPO-C plays a role in cell elongation and differentiation; suggesting a new aspect to the role of CWPO-C. CWPO-C may contribute to catabolism of plant hormones such as auxin, involved in cell elongation, differentiation and geotropism.
ポプラ CWPO-C とシロイヌナズナ CWPO-C ホモログのリグニンモデル化合物に対する酸化活性について

(九大農・院) 重藤潤、堤祐司、近藤隆一郎

第62回日本木材学会 2012.3

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Event date： 2012.3

Presentation type：Oral presentation (general)

Venue：札幌市 Country：Japan
CWPO-Cオーソログ、シロイヌナズナAtPrx71の植物成長調節機能

#周子堯、#坂井志帆、#山本怜奈、藤田弘毅、@重藤潤、堤祐司

第74回日本木材学会大会 2024.3

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Event date： 2024.3

Language：Japanese Presentation type：Oral presentation (general)

Venue：京都大学 Country：Japan
rCWPO-Cを用いた人工細胞壁上でのシリンギル型リグニンの成長反応促進因子の探索

#松久直文、藤田弘毅、堤祐司

第74回日本木材学会大会 2024.3

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Event date： 2023.3

Language：Japanese Presentation type：Oral presentation (general)

Venue：福岡 Country：Japan
CWPO-CオーソログであるAtPrx2、AtPrx25、AtPrx71をノックアウトしたシロイヌナズナ欠損体の成長とその制御作用

#宮田宗史朗、#山本怜奈、@重藤潤、#坂井志帆、#松井友里、藤田弘毅、堤祐司

第73回日本木材学会大会 2023.3

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Event date： 2023.3

Language：Japanese

Venue：福岡 Country：Japan
The cationic cell-wall-bound peroxidase (CWPO-C) involved in plant growth and lignification in Poplar

#Diego Yoshikay1, @Kaori Ohira, Koki Fujita, @Jun Shigeto, Yuji Tsutsumi

第73回日本木材学会大会 2023.3

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Event date： 2023.3

Language：English Presentation type：Oral presentation (general)

Venue：福岡 Country：Japan
CWPO-CおよびそのオーソログAtPrx71によるオーキシン不活性化を介した成長抑制機能の検討

#宮田宗史朗、#坂井志帆、#松井祐里、#横山裕亮、#Diego Yoshikay、藤田弘毅、@重藤潤、堤祐司

第67回リグニン討論会 2022.11

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Event date： 2022.11

Language：Japanese Presentation type：Oral presentation (general)

Venue：宮崎（オンライン） Country：Japan
化学、物理的刺激に対する Cupressus lusitanica 無菌発芽実生の防御機能

#春田黎暉, @藤田弘毅, @堤祐司

第72回日本木材学会大会 2022.3

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Event date： 2022.3

Language：Japanese Presentation type：Oral presentation (general)

Venue：名古屋・岐阜（オンライン） Country：Japan
CWPO-C による IAA 代謝を介した植物の成長制御

#宮田宗史朗, @藤田弘毅, @堤祐司, 雉子谷佳男

第72回日本木材学会大会 2022.3

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Event date： 2022.3

Language：Japanese Presentation type：Oral presentation (general)

Venue：名古屋・岐阜（オンライン） Country：Japan
rCWPO-C を用いたフェルラ酸を足場とするシリンギルリグニンセルロース複合体フィルムの創製

#松久直文,#田村剛, #川口なつみ, @藤田弘毅, @堤祐司

第72回日本木材学会大会 2022.3

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Event date： 2022.3

Language：Japanese Presentation type：Oral presentation (general)

Venue：名古屋・岐阜（オンライン） Country：Japan
タケアルカリリグニン分解物のα-グルコシダーゼ阻害活性

#林　純也、#廣島　将大、清水邦義、藤田弘毅、堤　祐司

第６５回リグニン討論会 2020.11

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Event date： 2020.11

Language：Japanese Presentation type：Oral presentation (general)

Venue：東京農業工業大学（オンライン開催） Country：Japan
植物ペルオキシダーゼ、CWPO-Cによる分化成長の制御

#横山裕亮、重藤　潤、大平香織、堤　祐司

第６8回日本木材学会大会 2019.3

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Event date： 2019.3

Language：Japanese Presentation type：Oral presentation (general)

Venue：函館 Country：Japan
Generation of lignin polymer models via dehydrogenative polymerization of coniferyl alcohol and sinapyl alcohol via plant peroxydase involved in lignification and analysis of the resulting DHPs by MALDI-TOF analysis International conference

Yuji Tsutsumi, Jun Shigeto, #Hiroki Honjo

29th International Conference of Polyphenols 2018.7

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Event date： 2018.7

Language：English Presentation type：Oral presentation (general)

Venue：Madison Country：Armenia

The mechanism of lignin dehydrogenative polymerization (DHP), made by mean of horseradish peroxidase (HRP), has been studied. Interestingly, HRP is efficient for guaiacyl type polymer formation (G-DHPs), but is not efficient in case of syringyl type DHPs (S-DHPs). It was previously demonstrate that lignification-related Arabidopsis peroxidases, AtPrx2, AtPrx25 and AtPrx71, and cationic cell-wall-bound peroxidase (CWPO-C) from the poplar are successful to oxidize syringyl- and guaiacyl-type monomers and larger lignin-like molecules. This is the reason why in the present study the DHP formation by means of these recombinant peroxidases was tested, and all these enzymes were successful for formation of both G-DHP and S-DHP in acceptable yields. CWPO-C led to S-DHP molecular size distribution similar to that of isolated lignins.
アルカリ蒸解タケリグニン由来カーボンファイバーの調製

河野陽平，藤田弘毅，堤　祐司，福永史

第６8回日本木材学会大会 2018.3

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Event date： 2018.3

Language：Japanese Presentation type：Oral presentation (general)

Venue：京都 Country：Japan
シロイヌナズナにおけるリグニンモノマー輸送体遺伝子の探索

武内真奈美, 堤祐司

第６8回日本木材学会大会 2018.3

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Event date： 2018.3

Language：Japanese Presentation type：Oral presentation (general)

Venue：京都 Country：Japan
CWPO-Cリコンビナントタンパクを用いたシナピルアルコール類縁体の脱水素重合

本庄裕貴，重藤潤, 堤祐司

第６8回日本木材学会大会 2018.3

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Event date： 2018.3

Language：Japanese Presentation type：Oral presentation (general)

Venue：京都 Country：Japan
PEG・バイサルファイト法タケ液化物を用いたエレクトロスピニング処理によるカーボンファイバーの調製

河野　陽平, 福永　史, 藤田弘毅, 堤祐司

第６2回リグニン討論会 2017.10

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Event date： 2017.10

Language：Japanese Presentation type：Oral presentation (general)

Venue：名古屋 Country：Japan
PEG・バイサルファイト法による液化したタケリグニンを原料とするカーボン材料

河野　陽平, 藤田弘毅, 堤祐司

第84回紙パルプ研究発表会 2017.6

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Event date： 2017.6

Language：Japanese Presentation type：Oral presentation (general)

Venue：東京 Country：Japan
モノテルペンを媒体とした連鎖的シグナリング　スギ・ヒノキ・メキシコイトスギ各培養細胞での比較

羽明駿, 藤田弘毅, 堤祐司

第６７回日本木材学会大会 2017.3

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Event date： 2017.3

Language：Japanese Presentation type：Oral presentation (general)

Venue：福岡 Country：Japan
Analysis of poplar CWPO-C gene expression using laser micro-dissection and RT-qPCR

Diego Yoshikay, 重藤潤, 堤祐司

第６７回日本木材学会大会 2017.3

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Event date： 2017.3

Language：English Presentation type：Oral presentation (general)

Venue：福岡 Country：Japan
CWPO-Cリコンビナントタンパクを用いたシリンギルリグニン様ポリマーの合成

本庄裕貴, 重藤潤, 堤祐司

第６７回日本木材学会大会 2017.3

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Event date： 2017.3

Language：Japanese Presentation type：Oral presentation (general)

Venue：福岡 Country：Japan
ソーダAQ処理によるタケリグニンの単離と物質変換

岡崎嘉孝, 藤田弘毅, 堤祐司, 高田依里, 増村竜史, 田畑研二, 亀井一郎

第６７回日本木材学会大会 2017.3

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Event date： 2017.3

Language：Japanese Presentation type：Oral presentation (general)

Venue：福岡 Country：Japan
PEG・バイサルファイト法による液化したタケリグニンを原料とするカーボンナノ材料の調製

段　夢弘, 河野陽平, 藤田弘毅, 堤祐司

第６７回日本木材学会大会 2017.3

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Event date： 2017.3

Language：Japanese Presentation type：Oral presentation (general)

Venue：福岡 Country：Japan
PEG・バイサルファイト法によるタケリグニン液化物から調製したカーボンファイバー

河野陽平, 段　夢弘, 藤田弘毅, 堤祐司

第６７回日本木材学会大会 2017.3

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Event date： 2017.3

Language：Japanese Presentation type：Oral presentation (general)

Venue：福岡 Country：Japan
スギの木化に関与するペルオキシダーゼの検索

松永佳奈, 重藤潤, 堤祐司

第６７回日本木材学会大会 2017.3

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Event date： 2017.3

Language：Japanese Presentation type：Oral presentation (general)

Venue：福岡 Country：Japan
マイクロダイセクション：リグニン単位間5-5型結合の探索

和田卓, 藤田弘毅, 堤祐司

第６７回日本木材学会大会 2017.3

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Event date： 2017.3

Language：Japanese Presentation type：Oral presentation (general)

Venue：福岡 Country：Japan
PEG・バイサルファイト法によるタケリグニン液化物から調製したポリウレタンフォームの特性評価

河野陽平, 段　梦弘, 藤田弘毅, 堤祐司

第６１回リグニン討論会 2016.10

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Event date： 2016.10

Language：Japanese Presentation type：Oral presentation (general)

Venue：京都 Country：Japan
シロイヌナズナ植物体および培養細胞を用いたリグニンモノマー輸送体候補遺伝子の発現解析

武内真奈美, 毛笠貴博, 堤祐司

第６１回リグニン討論会 2016.10

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Event date： 2016.10

Language：Japanese Presentation type：Oral presentation (general)

Venue：京都 Country：Japan
マイクロダイセクション：ポプラ１年輪内のリグニン単位間結合変化の解析

和田卓, 藤田弘毅, 堤祐司

第６１回リグニン討論会 2016.10

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Event date： 2016.10

Language：Japanese Presentation type：Oral presentation (general)

Venue：京都 Country：Japan
Transcriptional analysis of Poplar CWPO-C in different cell types and organs

Diego Yoshikay, 大平香織, 重藤潤, 堤祐司

第６１回リグニン討論会 2016.10

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Event date： 2016.10

Language：English Presentation type：Oral presentation (general)

Venue：京都 Country：Japan
In vitro polymerization of lignin monomer using the plant peroxidases involved in lignification International conference

Yuji Tsutsumi, Jun Shigeto, Hiroki Honjyo

Oxizymes 2016 2016.7

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Event date： 2016.7

Language：English Presentation type：Oral presentation (general)

Venue：Wageningen Country：Netherlands
Bamboo Refinery: Production of phenolic compounds from lignin by catalytic hydrogenation and fermentation of xylose International conference

Ichiro Kamai, Eri Takata, Yuji Tsutsumi

Lignobiotech IV 2016.6

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Event date： 2016.6

Language：English Presentation type：Oral presentation (general)

Venue：Madrid Country：Spain
In vitro evaluation of the lignin forming ability of plant peroxidases involved in lignification International conference

Yuji Tsutsumi, Jun Shigeto, Hiroki Honjyo

Lignobiotech IV 2016.6

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Event date： 2016.6

Language：English Presentation type：Oral presentation (general)

Venue：Madrid Country：Spain
形質転換ポプラ及びシロイヌナズナを用いたポプラペルオキシダーゼCWPO-Cの機能探索

大平香織, 重藤　潤, 堤祐司

第６６回日本木材学会大会（名古屋） 2016.3

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Event date： 2016.3

Language：Japanese

Venue：名古屋大学 Country：Japan
シロイヌナズナ管状要素形成におけるリグニン前駆体輸送と木化関連遺伝子の転写解析

毛笠貴博, 梅村早紀, 武内真奈美, 田村美帆, 渡辺敦史, 重藤潤, 堤祐司

第６６回日本木材学会大会（名古屋） 2016.3

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Event date： 2016.3

Language：Japanese

Venue：名古屋大学 Country：Japan
メキシコイトスギ培養細胞とヒノキ培養細胞の気相シグナル伝達の比較

羽明　駿, 藤田弘毅, 堤祐司

第６６回日本木材学会大会（名古屋） 2016.3

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Event date： 2016.3

Language：Japanese

Venue：名古屋大学 Country：Japan
シロイヌナズナペルオキシダーゼ、AtPrx2, 25, 71の発現解析による機能推定

鎌田　政諒, 大平　香織, 重藤潤, 堤祐司

第60回リグニン討論会 2015.11

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Event date： 2015.11

Language：Japanese

Venue：筑波大学 Country：Japan
揮発性モノテルペンを用いたCupressus lusitanicaの情報伝達

羽明　駿, 藤田弘毅, 堤祐司

木材学会九州支部 2015.10

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Event date： 2015.10

Language：Japanese Presentation type：Oral presentation (general)

Venue：大分 Country：Japan
ハクサンハタザオによるCd/Pb複合重金属の吸収

陶　揚洋, 藤田弘毅, 堤祐司

木材学会九州支部 2015.10

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Event date： 2015.10

Language：Japanese Presentation type：Oral presentation (general)

Venue：大分 Country：Japan
レーザーマイクロダイセクションを用いたポプラ木化進行過程におけるリグニン解析

堤祐司, 和田　卓

日本植物学会 2015.9

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Event date： 2015.9

Language：Japanese Presentation type：Oral presentation (general)

Venue：新潟 Country：Japan
シロイヌナズナ培養細胞管状要素誘導中に発現するモノリグノール輸送体の探索

堤祐司

第65回木材学会(東京) 2015.3

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Event date： 2015.3

Language：Japanese

Venue：船堀 Country：Japan

植物細胞壁成分であるリグニンは構成成分であるモノリグノールの細胞内での生合成、細胞外への輸送、細胞壁での重合の3段階で生成されるが、モノリグノールの輸送機構は生合成経路と比べて未解明な部分が多い。シロイヌナズナ培養細胞系は細胞を管状要素に分化誘導することで木化途中の細胞を同調的に得ることができるためモノリグノール輸送体の探索に大変有用だと思われる。そこで本研究ではモノリグノール輸送体の候補遺伝子を探索することを目的として、シロイヌナズナ培養細胞T87株の管状要素分化誘導系の確立を試みた。その結果、前培養培地のMS培地濃度を２～３倍に希釈すると分化率が上昇し、前培養培地、誘導培地共に放散を添加すると分化が阻害され、最適化した誘導条件で約１週間培養すると20%程度の分化率を得ることができた。現在誘導培地中のモノリグノール量の測定とモノリグノール輸送体候補遺伝子の発現パターンの解析を行っている。
リグニン生合成に関与するペルオキシダーゼによるシリンギル型モノマー脱水素重合

重藤潤, 堤祐司

第65回日本木材学会大会 2015.3

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Event date： 2015.3

Language：Japanese

Venue：東京 Country：Japan
ポプラの組織別におけるCWPO-C転写解析

堤祐司, 重藤　潤, 髙尾龍之介, 大平香織

日本木材学会大会 2015.3

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Event date： 2015.3

Language：Japanese Presentation type：Symposium, workshop panel (public)

Venue：東京 Country：Japan

ポプラ由来のペルオキシダーゼであるCWPO-Cはリグニンポリマーならびにポリマーへ高い酸化能を有することから木化への関与が示唆された。またCWPO-C抗体を用いた二次木部の免疫標識はセルコーナー、細胞間層、放射柔組織に観察された。一方、シロイヌナズナ形質転換体を用いたCWPO-Cプロモーター解析では、CWPO-Cが分化に寄与する可能性が示されたことから、CWPO-Cの多様な機能が予想された。そこで本研究では、ポプラの植物体から木化や分化を行っている微細組織を採取し、CWPO-Cの転写解析を行うことでCWPO-Cの機能について考察を試みた。
ニセアカシアのCd蓄積機構

若林昌嘉, 堤祐司

第65回木材学会(東京) 2015.3

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Event date： 2015.3

Language：Japanese

Venue：タワーホール船堀　(東京) Country：Japan

植物に土壌中の重金属を吸収させるファイトレメディエーションは、低環境負荷かつ低コストに重金属汚染を浄化できる技術として期待されている。これまでに多数の草本植物がCdの超蓄積植物として報告されており、根の内皮にCdを局在させて中心柱への侵入を防ぐことや、ファイトケラチチンとの結合によりCdを無毒化させることが知られている。当研究室が報告した超蓄積植物であるニセアカシアは草本植物と比べて広範囲から多量のCdを蓄積すると見込まれる。本研究ではニセアカシアの高いCd耐性機構に関する知見を得るため、Micro X-ray Fluorescence (μ-XRF)分析による根のCd局在観察と、X-ray Absorption Fine Structure (XAFS)分析によるCd結合元素の検討を行った。μ-XRF分析の結果、Cdは根の表皮近くに局在しつつ中心柱内部にも存在していた。またXAFS分析によりニセアカシアの根においてCdは硫黄と結合している可能性が示された。また、活性酸素除去酵素の活性測定実験の結果、活性酸素除去がCd耐性に寄与している可能性は低いことが示唆された。
Screening of monolignol transport protein in Arabidopsis thaliana International conference

Yuji Tsutsumi, Manami Takeuchi

International Symposium on Wood Science and Technology 2015 2015.3

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Event date： 2015.3

Language：English

Venue：Tokyo Country：Japan

Screening of monolignol transport protein in Arabidopsis thaliana

Monolignols are synthesized in the cytosol and transported to the cell wall to biosynthesize linign. It was reported that one of ATP-binding cassette (ABC) proteins, AtABCG29, is involved in the transportation of p-coumaryl alcohol, however, there have been no report on other monolignols for guaiacyl and syringyl lignin, which are major constitute units of angiosperm lignin. In this study, 9 genes including ABC proteins were selected as monoligol transport candidates among the genes those expressions were largely changed during the tracheary element differentiation of Arabidopsis cell culture, and T-DNA insert mutants of these genes were subjected to lignin analyses. There were no difference in both lignin content and S/G ratio between wild type and each knockout mutant. The expression analysis of each gene in the several organs did not show specificity in the stem unlike ABCG29. These results suggest that the candidate genes may not be involved in monolignol transportation in the Arabidopsis stem.
In the next screening we focus on evolutionally altered genes. Vascular plants have developed vascular bundle to conduct water and support their own body. Along with this evolution, lignin biosynthesis system had been also evolved. ABC transporter genes were compared between Arabidopsis thaliana as vascular plant and Physcomitrella patens as nonvascular plant, and 66 ABC proteins were found to be vascular plant specific. Further 28 ABCG subfamily genes among them were chosen as candidates, because some ABCG subfamily proteins have been reported to be related to secondary metabolism. The monolignol transport assay using transformed yeast with candidate gene is also performed to screen potential monolignol exporter.
Selective production of tetrahydrofurfuryl alcohol and xylitol from napier grass by a hydrothermal process with phosphorus oxoacids followed by catalytic hydrogenation International conference

Eri Takata, Tatsushi Tsuruoka, Ken Tsutsumi, Kenji Tabata, Yuji Tsutsumi

Lignobiotech III 2014.10

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Event date： 2014.10

Language：English Presentation type：Oral presentation (general)

Venue：Concepción Country：Chile

Other Link： http://www.lignobiotech.cl/
Expression and subcellular localization analysis of poplar cationic cell-wall-bound peroxidase and its Arabidopsis putative homologs involved in lignification International conference

Yuji Tsutsumi, Jun Shigeto, Kaori Ohira, Ryunosuke Takao, Ｍａｓａａｋｉ　Kamada

Lignobiotech III 2014.10

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Event date： 2014.10

Language：English Presentation type：Oral presentation (general)

Venue：Concepción Country：Chile

Genetic engineering of gene which encodes peroxidase responsible for lignin polymerization is expected as an effective technique to change lignin content and structure in plant, but it is still far from the industrial application. This is because of the lack of fundamental knowledge about physiological functions of peroxidases. Limited number of peroxidases involved in lignification were identified in some plants, and little is known about their tissue-specific expression and subcellular localization pattern.
Cationic cell-wall-bound peroxidase (CWPO-C) from poplar has been verified to serve oxidation of both monolignols and lignin polymer, for the first time [1, 2]. This oxidation property is good as the enzyme responsible for lignin polymerization. In addition, AtPrx-2, 25 and 71 which have high amino acid similarity to CWPO-C were shown to contribute to stem lignification of Arabidopsis [3], and have CWPO-C-like oxidation property. In this study, tissue-specific expression and localization of CWPO-C and its Arabidopsis putative homologs, AtPrx-2, 25 and 71, were investigated. Promoter assay using transgenic Arabidopsis line carrying a CWPO-C promoter driven β-glucuronidase construct revealed that CWPO-C expressed in young developing tissues including protoxylem, except root tip. We also generated transgenic Arabidopsis T87 cultured cell overexpressed CWPO-C-EGFP fusion protein, and the fluorescence was observed in helical cell wall thickening of the cell differentiated into tracheary element. This presentation will discuss about the role of each peroxidase in the lignification process from a viewpoint of expression and subcellular localization patterns of CWPO-C, AtPrx-2, 25 and 71.

References
[1] Aoyama W, Sasaki S, Matsumura S, Hirai H, Tsutsumi Y, et al. (2002), J Wood Sci 48: 497–504.
[2] Sasaki S, Nishida T, Tsutsumi Y, Kondo R (2004), FEBS Lett 562: 197–201.
[3] Shigeto J, Kiyonaga Y, Fujita K, Kondo R, Tsutsumi Y (2013), J Agric Food Chem 61: 3781–3788.

Other Link： http://www.lignobiotech.cl/
高分子リグニン酸化能をもつポプラペルオキダーゼCWPO-C の発現と局在解析

堤祐司, 大平香織, 重藤潤

日本植物学会第78回大会（川崎） 2014.9

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Event date： 2014.9

Language：Japanese Presentation type：Oral presentation (general)

Venue：明治大学　生田キャンパス Country：Japan
Characterization of Plant Peroxidases which Involved in Arabiodpsis Stem Lignification International conference

Jun Shigeto, Yuji Tsutsumi

XXVIIth International Conference on Polyphenols & 8th Tannin Conference 2014.9

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Event date： 2014.9

Language：English Presentation type：Oral presentation (general)

Venue：Symposion and Noyori Conference Hall, Nagoya University Country：Japan
OXIDATION ACTIVITIES OF PLANT PEROXIDASES INVOLVED IN LIGNIFICATION International conference

堤祐司, 重藤　潤

Oxizymes 2014.7

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Event date： 2014.7

Language：English Presentation type：Oral presentation (general)

Venue：University of Natural Resources and Life Sciences, Vienna Country：Austria

Lignin is a main component of vascular plant cell walls and possesses a complex and irregular structure. In angiosperms, lignins consist mainly of two monolignols, coniferyl (4-hydroxy-3-methoxycinnamyl) and sinapyl (3,5-dimethoxy-4-hydroxycinnamyl) alcohols, which polymerize through at least five different linkage types and result in 4-hydroxy-3-methoxyphenyl (guaiacyl) and 3,5-dimethoxy-4-hydroxyphenyl (syringyl) units, respectively. Monolignols are supplied to the cell wall and polymerized to fill, together with hemicellulose, the spaces between cellulose microfibrils; this polymerization proceeds through oxidative coupling catalyzed by plant peroxidases. Based on the ‘‘End-wise’’ polymerization process, monolignol radicals can be coupled to a growing lignin polymer to produce a lignin macromolecule [1].
However, most known plant peroxidases, including horseradish peroxidase (HRP) and AtPrx53, can only oxidize p-coumaryl and coniferyl alcohols. This characteristic difference has led to controversy regarding the oxidation mechanism of sinapyl alcohol, lignin oligomers and polymers by plant peroxidases. Until now, CWPO-C, a peroxidase isozyme from Populus alba L., has only been verified to serve combined oxidation activity for sinapyl alcohol, lignin ligomers and polymer [2].The tyrosine 74 and tyrosine 177 located on the surface of CWPO-C, instead of the heme pocket, take a central role in the oxidation of substrates [3].
Previously, we has focused on seven Arabidopsis plant peroxidases selected using amino acid similarities to CWPO-C as a probe and found that AtPrx2 or AtPrx25 deficiency led both decreased total lignin content and altered lignin structure, including cell wall thinning in the stem. In addition, AtPrx71 deficiency led an altered stem lignin structure, although the lignin content is not decreased. These results provided in vivo evidence that AtPrx-2, 25, and 71 are involved in Arabidopsis stem lignification [4].
The present study explored the oxidation activities of three plant peroxidases, AtPrx-2, 25, and 71, which have been already shown to be involved in lignification Arabidopsis stem. Recombinant proteins of these peroxidases (rAtPrxs) were produced in Escherichia coli as inclusion bodies and successfully refolded to yield their active forms. The specific activities of rAtPrx-2, 25 and 71 toward two syringyl compounds (2,6-dimethoxyphenol and syringaldazine) were higher than that of HRP-C and rAtPrx53. Interestingly, rAtPrx2 and rAtPrx71 oxidized syringyl compounds more efficiently than guaiacol. Moreover, assays with ferrocytochrome c showed that AtPrx2, AtPrx25, and AtPrx71 possessed the ability to oxidize large molecules. This characteristic presumably originate in a protein radical. These results provide the evidence that the plant peroxidases responsible for lignin polymerization are able to directly oxidize all lignin precursors. We will also present an assay of dehydrogenative polymer formed from monolignols with rAtPrxs.

[1] Sarkanen KV (1971) Precursors and their polymerization. In: Sarkanen KV and Ludwig CH (eds) Lignins, Occurrence, Formation, Structure and Reactions. Wiley-Interscience, New York, pp. 95–163.
[2] Aoyama W, Sasaki S, Matsumura S, Hirai H, Tsutsumi Y, et al. (2002) Sinapyl alcohol-specific peroxidase isoenzyme catalyzes the formation of the dehydrogenative polymer from sinapyl alcohol. J. Wood Sci 48: 497–504.
[3] Shigeto J, Itoh Y, Tsutsumi Y, Kondo R (2012) Identification of Tyr74 and Tyr177 as substrate oxidation sites in cationic cell wall-bound peroxidase from Populus alba L. FEBS J 279: 348-357.
[4] Shigeto J, Kiyonaga Y, Fujita K, Kondo R, Tsutsumi Y (2013) Putative Cationic Cell-Wall-Bound Peroxidase Homologues in Arabidopsis , AtPrx2, AtPrx25, and AtPrx71, Are Involved in Lignification. J Agric Food Chem 61: 3781–3788
テルペンシンターゼによるシグナル伝達物質のde novo生産及び伝達メカニズム

神戸　良, 藤田弘毅, 堤祐司

第64回　日本木材学会大会（松山） 2014.3

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Event date： 2014.3

Language：Japanese Presentation type：Oral presentation (general)

Venue：愛媛大学城北キャンパス　共通教育講義棟 Country：Japan
CWPO-Cの転写およびプロモーター解析

大平香織, Kasturi Banerjee, 重藤潤, 堤祐司

第64回　日本木材学会大会（松山） 2014.3

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Event date： 2014.3

Language：Japanese Presentation type：Oral presentation (general)

Venue：愛媛大学城北キャンパス　共通教育講義棟 Country：Japan
リグニンを高分子化する植物ペルオキシダーゼの酸化能

重藤潤, 堤祐司

第58回リグニン討論会 2013.11

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Event date： 2013.11

Language：Japanese Presentation type：Oral presentation (general)

Venue：サンポートホール高松 Country：Japan
Cupressus lusitanica 培養細胞が気相中に発散するモノテルペンの役割　（口頭）

神戸　良, 藤田弘毅, 芦谷　竜矢, 堤祐司

第５７回　香料・テルペンおよび精油化学に関する討論会 2013.10

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Event date： 2013.10

Language：Japanese Presentation type：Oral presentation (general)

Venue：埼玉大学 Country：Japan
リンオキソ酸水熱反応によるネピアグラスからのキシラン分離とフラン誘導体への変換

高田依里, 鶴岡達志, 岸尾昌典, 堤健, 田畑研二, 堤祐司

化学工学会第45回秋季大会 2013.9

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Event date： 2013.9

Language：Japanese Presentation type：Oral presentation (general)

Venue：岡山市 Country：Japan
〜ネピアグラスの統合バイオリファイナリー〜リンオキソ酸水熱処理による単糖生成とモノマー変換技術

高田依里, 鶴岡達志, 堤健, 田畑研二, 堤祐司

第20回日本木材学会九州支部大会 2013.9

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Event date： 2013.9

Language：Japanese Presentation type：Oral presentation (general)

Venue：福岡市 Country：Japan
Cupressus lusitanica 培養細胞のシグナル伝達に関与する炭化水素モノテルペンの経時的特性（口頭）

神戸　良, 藤田弘毅, 堤祐司

第20回日本木材学会九州支部大会(福岡) 2013.9

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Event date： 2013.9

Language：Japanese Presentation type：Oral presentation (general)

Venue：九州大学 Country：Japan
Air born defense signal transduction cascade with monoterpenes on Cupressus lusitanica culture cells (poster) International conference

Ryo Kanbe, Koki Fujita, Tatsuya Yagi, Ransika De Alwis, Tatuya Ashitani, Yuji Tsutsumi

TERPNET2013 2013.6

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Event date： 2013.6

Language：English Presentation type：Oral presentation (general)

Venue：Kolymvari, Crete, Greece Country：Japan
Air born defense signal transduction cascade with monoterpenes on Cupressus lusitanica culture cells (poster) International conference

Ryo Kanbe, Koki Fujita, Tatsuya Yagi, Ransika De Alwis, Tatuya Ashitani, Yuji Tsutsumi

TERPNET2013 2013.6

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Event date： 2013.6

Language：English Presentation type：Oral presentation (general)

Venue：Kolymvari, Crete, Greece Country：Japan
Cuplessus lusitanica 培養細胞における炭化水素モノテルペンを介したシグナル伝達(口頭)

神戸　良, 藤田弘毅, 堤祐司

第63回　日本木材学会大会 2013.3

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Event date： 2013.3

Language：Japanese Presentation type：Oral presentation (general)

Venue：岩手大学 Country：Japan
Effect of pretreatment on the production of monosaccharides from napier grass by hydrothermal reaction with acid catalyst International conference

Eri Takata, Ken Tsutsumi, Yuji Tsutsumi, Kenji Tabata

Lignobiotech II Symposium 2012.10

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Event date： 2012.10

Language：English Presentation type：Oral presentation (general)

Venue：Fukuoka Country：Japan
Oxidation activity of three Arabidopsis peroxidases, AtPrx2, AtPrx25 and AtPrx71, for monolignol model compounds (poster) International conference

Jun Shigeto, Mariko Nagano, Yuji Tsutsumi

Lignobiotech II Symposium 2012.10

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Event date： 2012.10

Language：English Presentation type：Oral presentation (general)

Venue：Fukuoka Country：Japan
Three putative CWPO-C homologs of Arabidopsis peroxidases, AtPrx2, AtPrx25 and AtPrx71, are involved in lignifications International conference

Jun Shigeto, Yuji Tsutsumi, Yoshitaka Itoh

Lignobiotech II Symposium 2012.10

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Event date： 2012.10

Language：English Presentation type：Oral presentation (general)

Venue：Fukuoka Country：Japan
酸触媒と水熱反応による草本バイオマスの糖化

高田依里, 堤健, 堤祐司, 田畑研二

化学工学会第44回秋季大会 2012.9

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Event date： 2012.9

Language：Japanese Presentation type：Oral presentation (general)

Venue：仙台市 Country：Japan
シロイヌナズナ CWPO-C ホモログのダブルノックアウトによるリグニン生合成抑制

重藤潤, 伊藤義隆, 堤祐司

第76回日本植物学会大会 2012.9

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Event date： 2012.9

Language：Japanese Presentation type：Oral presentation (general)

Venue：札幌市 Country：Japan
Recycling of Bovine Meat and Bone Meal and Inactivation of BSE Prion by Sub-Critical Water Treatment. International conference

Yuji Tsutsumi, Eri Takata, Yuichi Murayama

10th International Symposium on Supercritical Fluids. 2012.5

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Event date： 2012.5

Language：English Presentation type：Oral presentation (general)

Venue：San Francisco Country：United States
シロイヌナズナにおいてリグニン生合成へ関与するペルオキシダーゼの基質酸化能の解析

(九大農・院) ○重藤潤、堤祐司、近藤隆一郎

第53回日本植物生理学会 2012.3

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Event date： 2012.3

Presentation type：Oral presentation (general)

Venue：京都市 Country：Japan
ニセアカシア（Robinia pseudoacasia）のカドミウム蓄積機構

（九大院・生資環）濱口大空、（九大院・農）堤祐司、近藤隆一郎

第62回日本木材学会 2012.3

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Event date： 2012.3

Presentation type：Oral presentation (general)

Venue：札幌市 Country：Japan
シロイヌナズナ CWPO-C ホモログのダブルノックアウトがリグニン生合成に与える影響

(九大院生資環)○伊藤義隆、(九大農学院)重藤潤、堤祐司、近藤隆一郎

第62回日本木材学会 2012.3

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Event date： 2012.3

Presentation type：Oral presentation (general)

Venue：札幌市 Country：Japan
細胞壁結合型ペルオキシダーゼ CWPO-C の基質酸化におけるチロシン残基の重要性

(九大院農)重藤潤、伊藤義隆、堤祐司、近藤隆一郎

第７５回日本植物学会 2011.9

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Event date： 2011.9

Presentation type：Oral presentation (general)

Venue：東京大学駒場キャンパスⅠ Country：Japan
高分子リグニン酸化能をもつ CWPO-C における基質酸化部位の同定

(九大院農)重藤潤、伊藤義隆、堤祐司、近藤隆一郎

第５６回リグニン討論会 2011.9

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Event date： 2011.9

Venue：山形大学農学部 Country：Japan
シロイヌナズナ CWPO-C ホモログのダブルノックアウト変異体解析

(九大院生資環)伊藤義隆、重藤潤、堤祐司、近藤隆一郎

第５６回リグニン討論会 2011.9

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Event date： 2011.9

Presentation type：Oral presentation (general)

Venue：山形 Country：Japan
ポプラペルオキシダーゼアイソザイムCWPO-C の基質酸化機構と木化への関与

(九大院農) 重藤潤、堤祐司、近藤隆一郎　(九大院生資環) 伊藤義隆

第18回日本木材学会九州支部大会 2011.8

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Event date： 2011.8

Presentation type：Oral presentation (general)

Venue：鹿児島大学 Country：Japan
亜臨界水による牛肉骨粉処理物の肥料としての再資源化

（九大農学院）福田　淳子、高田　依里、房　賢貞、山川　武夫、堤　祐司

2011年度日本土壌肥料学会 2011.8

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Event date： 2011.8

Venue：筑波 Country：Japan
インビトロアッセイ系によるクロマツのマツノザイセンチュウ抵抗性評価

（九大院生資環）張阜嘉、(福岡県森林林業技術センター)森康浩、（九大院農）堤祐司、（九大院農）近藤　隆一郎

第122回日本森林学会大会 2011.3

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Event date： 2011.3

Venue：静岡大学 Country：Japan
ペルオキシダーゼアイソザイムCWPO-Cの基質酸化機構とリグニン重合への関与

（九大院農）重藤潤、堤祐司、近藤隆一郎

第52回植物生理学会 2011.3

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Event date： 2011.3

Venue：仙台市 Country：Japan
ニセアカシア（Robinia pseudoacasia）のカドミウム蓄積機構

（九大院・生資環）○濱口大空、（九大院・農）堤祐司、近藤隆一郎

第61回日本木材学会 2011.3

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Event date： 2011.3

Venue：京都大学 Country：Japan
Recycling of bovine meat and bone meal by sub-critical water treatment International conference

Yuji Tsutsumi, H. Bang, Y. Murayama, M. Yoshioka, T. Noguchi

Prion, Japan & Canada 2010.11

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Event date： 2010.11

Presentation type：Oral presentation (general)

Venue：Tokyo Country：Japan
Bioremediation of DDT by Pleurotus ostreatus spent mushroom waste: involvement of ligninolytic enzymes and application in historically contaminated soil.

Purnomo AS, Mori T, Takata E, Tsutsumi Y, Kondo R.

55th Lignin Symposium 2010.10

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Event date： 2010.10

Venue：Kyoto Country：Japan
牛肉骨粉の亜臨界水処理による生成物

房　賢貞、福田　淳子、近藤　隆一郎、堤　祐司

2009年度日本農芸化学会関西・中四国・西日本支部、日本栄養・食糧学会九州・沖縄支部日本食品科学工学会西日本支部、3学会5支部合同大会 2009.10

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Event date： 2009.10 - 2010.10

Venue：沖縄 Country：Japan
ニセアカシアの重金属蓄積能の解明

(九大院･農）河口佑子、近藤隆一郎、堤祐司

日本木材学会 2009.3

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Event date： 2009.3

Presentation type：Oral presentation (general)

Venue：松本市 Country：Japan
木材腐朽菌によるスギ間伐材の腐朽処理

(九大院･生資環）川添裕幸、（九大院･農）堤祐司、近藤隆一郎

日本木材学会 2009.3

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Event date： 2009.3

Presentation type：Oral presentation (general)

Venue：松本市 Country：Japan
レーザーマイクロダイセクションによる木化段階別リグニン生合成遺伝子の転写解析と化学分析

(九大院･農) 田中奏、藤田弘毅、黒田健一、堤祐司、近藤隆一郎

日本木材学会 2009.3

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Event date： 2009.3

Presentation type：Oral presentation (general)

Venue：松本市 Country：Japan
Effects of combinational treatment with ethephon and indole-3-butyric acid on adventitious root formation of Pinus thunbergii cuttings. International conference

Yasuhiro MORI, Fumihiko MIYAHARA, Yuji TSUTSUMI, Ryuichiro KONDO

2008.6

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Event date： 2008.6

Presentation type：Oral presentation (general)

Venue：Madrid, Spain Country：Spain
レーザーマイクロダイセクションによる微量組織でのリグニン生合成遺伝子転写解析およびリグニン分析

（九大院生資環）田中奏、藤田弘毅、黒田健一、堤祐司、近藤隆一郎

日本木材学会 2008.3

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Event date： 2008.3

Presentation type：Oral presentation (general)

Venue：つくば市 Country：Japan
Involvement of DDT-dechlorinase in degradation of 1,1,1-Trichloro-2,2-bis(4-chlorophenyl)Ethane (DDT)

(Faculty of Agriculture, Kyushu University)Adi Setyo Purnomo, Ichiro Kamei, Yuji Tsutsumi and Ryuichiro Kondo

日本木材学会 2008.3

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Event date： 2008.3

Presentation type：Oral presentation (general)

Venue：つくば市 Country：Japan
MnPによる異常プリオンタンパクの分解・不活化と分解機構

(九大院生資環)高田　衣里、（動物衛生県）松浦　裕一、毛利　資郎（九大院農）堤　祐司、近藤隆一郎

日本木材学会 2008.3

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Event date： 2008.3

Presentation type：Oral presentation (general)

Venue：つくば市 Country：Japan
シロイヌナズナにおけるCWPO-Cホモログの機能解析

（九大院・生資環）清永裕子、佐々木慎弥、（九大・農）堤祐司、近藤隆一郎

日本木材学会 2008.3

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Event date： 2008.3

Presentation type：Oral presentation (general)

Venue：つくば市 Country：Japan
ニセアカシアの重金属蓄積能の解明

(九大院･農）河口佑子、近藤隆一郎、堤祐司

日本木材学会 2008.3

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Event date： 2008.3

Presentation type：Oral presentation (general)

Venue：松本市 Country：Japan
木材腐朽菌によるスギ間伐材の腐朽処理

(九大院･生資環）川添裕幸、（九大院･農）堤祐司、近藤隆一郎、

日本木材学会 2008.3

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Event date： 2008.3

Presentation type：Oral presentation (general)

Venue：松本市 Country：Japan
レーザーマイクロダイセクションによる木化段階別リグニン生合成遺伝子の転写解析と化学分析

(九大院･農) 田中奏、藤田弘毅、黒田健一、堤祐司、近藤隆一郎

日本木材学会 2008.3

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Event date： 2008.3

Presentation type：Oral presentation (general)

Venue：松本市 Country：Japan
ポプラペルオキシダーゼアイソザイムCWPO-Cの機能解析

清永裕子・佐々木慎弥・堤祐司・近藤隆一郎

第52回リグニン討論会 2007.11

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Event date： 2007.11

Venue：宇都宮市 Country：Japan
プラペルオキシダーゼの遺伝子およびタンパク構造の多様性

江藤祐・重松幹二・堤祐司・近藤隆一郎

第52回リグニン討論会 2007.11

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Event date： 2007.11

Venue：宇都宮市 Country：Japan
マツ材線虫病抵抗性クロマツの挿し木増殖と抵抗性要因

森康浩・堤祐司・近藤隆一郎

日本木材学会九州支部会 2007.11

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Event date： 2007.11 - 2008.11

Venue：久留米市 Country：Japan
ポプラペルオキシダーゼ遺伝子の網羅的単離と転写解析

江藤祐・堤祐司・近藤隆一郎

第57回日本木材学会 2007.8

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Event date： 2007.8

Venue：広島市 Country：Japan
マツ材線虫病抵抗性と感受性のクロマツ抽出成分を使ったマツノザイセンチュウのバイオアッセイ

森康浩・堤祐司・近藤隆一郎

日本森林学会 2007.4

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Event date： 2007.4

Venue：福岡市 Country：Japan
リグニンの高分子化を担う植物ペルオキシダーゼの酸化能と酸化機構

重藤潤, 堤祐司

第55回日本植物生理学会年会 2014.3

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Language：Japanese Presentation type：Symposium, workshop panel (public)

Country：Japan
広葉樹リグニン特異的ペルオキシダーゼ遺伝子の単離と局在

佐々木慎弥、青山渉、小川直人、西田友昭、堤　祐司、近藤隆一郎

第53回　日本木材学会大会 2003.3

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Presentation type：Oral presentation (general)

Venue：九州産業大学（福岡） Country：Japan

Syringyl lignin specific peroxidase isoenzyme -Isolation of cDNA and localization of its protein in poplar tree-.
Shinya Sasaki, Wataru Aoyama, Naoto Ogawa, Tomoaki Nishida, Yuji Tsutsumi, Ryuichiro Kondo
53rd Annual Meeting of the Japan Wood Reseach Society
March, 2003
Kyushu Sangyo University (Fukuoka)
白色腐朽菌MG-60株の産生するマンガンペルオキシダーゼの発現特性

大黒千映子、須原弘登、杉山智規、堤祐司、近藤隆一郎

第53回　日本木材学会大会 2003.3

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Presentation type：Oral presentation (general)

Venue：九州産業大学（福岡） Country：Japan

Characterization and expression of manganese peroxidases produced by white-rot fungus MG-60.
Chieko Daikoku, Hiroto Suhara, Yuji Tsutsumi, Ryuichiro Kondo53rd Annual Meeting of the Japan Wood Reseach Society
March, 2003
Kyushu Sangyo University (Fukuoka)
白色腐朽菌MG-60株の耐塩性機構

杉山智規、堤祐司、近藤隆一郎

第53回　日本木材学会大会 2003.3

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Presentation type：Oral presentation (general)

Venue：九州産業大学（福岡） Country：Japan

Salt tolerance mechanisms for white-rot fungus MG-60.
Tomonori Sugiyama, Yuji Tsutsumi, Ryuichiro Kondo
53rd Annual Meeting of the Japan Wood Reseach Society
March, 2003
Kyushu Sangyo University (Fukuoka)
ニセアカシア４CL遺伝子の検索と発現特性

西口朋子、西田友昭、堤祐司、近藤隆一郎

第53回　日本木材学会大会 2003.3

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Presentation type：Oral presentation (general)

Venue：九州産業大学（福岡） Country：Japan

Cloning and expression of 4-coumarate:CoA ligases from black locust.
Tomoko Nishiguchi, Kazuhiko Fukushima, Tomoaki Nishida, Yuji Tsutsumi, Ryuichiro Kondo
53rd Annual Meeting of the Japan Wood Reseach Society
March, 2003
Kyushu Sangyo University (Fukuoka)
リグニンの脱水素重合における細胞壁結合生ペルオキシダーゼの役割と局在

佐々木慎弥、小川直人、青山渉、西田友昭、堤　祐司、近藤隆一郎

第43回　日本植物生理学会シンポジウム 2003.4

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Presentation type：Oral presentation (general)

Venue：近畿大学（奈良） Country：Japan

Characterization and localization of cell wall peroxidase responsible for the dehydrogenative polymerization of lignin.
Shinya Sasaki, Naoto Ogawa, Wataru Aoyama, Tomoaki Nishida, Yuji Tsutsumi, Ryuichiro Kondo
43rd Symposium of the Japanese Society for Plant Physiologists
March, 2003
Kinki University (Nara), Jpan
Characterization of poplar cell wall peroxidase responsible for the dehydrogenative polymerization of lignin. International conference

Shinya Sasaki, Tomoaki Nishida, Yuji Tsutsumi, Ryuichiro Kondo

12th International Symposium on Wood and Pulping Chemistry 2003.6

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Presentation type：Oral presentation (general)

Venue：Madison Country：United States
リグニンの脱水素重合とペルオキシダーゼの機能 Invited

堤　祐司

平成１５年度　日本木材学会・組織と材質研究会シンポジウム 2003.8

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Presentation type：Oral presentation (general)

Venue：京都大学（宇治市） Country：Japan

Mechanisms of dehydrogenative polymerization of lignin and ligninfication-specific peroxidase isoenzyme.
Yuji Tsutsumi
Symposium on the wood structure (2003)
Kyoto University (Uji), Japan
ニセアカシア4-coumarate:CoA ligaseの基質特異性とリグニン生合成における役割

堤　祐司、浜田　勝義、西口　朋子、福島　和彦、西田　友昭、近藤　隆一郎

第６７回日本植物学会大会 2003.9

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Presentation type：Oral presentation (general)

Venue：札幌 Country：Japan

Linginn biodynthesis and 4-coumarate:CoA ligase in black locust.
Yuji Tsutsumi, Kutsuyoshi Hamada, Tomoko Nishiguchi, Kazuhiko Fukushima, Tomoaki Nishida, Ryuichiro Kondo
67th Annual Meeting of The Botanical Society of Japan
September, 2003
Sapporo, Japan
シナップ酸を投与したポプラカルスにおけるシリンギルリグニン生合成の活性化

浜田　勝義、西田　友昭、福島　和彦、山内　一慶、堤　祐司

第６７回日本植物学会大会 2003.9

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Presentation type：Oral presentation (general)

Venue：札幌 Country：Japan

Syringyl ignin biodynthesis in poplar callus administered with sinapic acid.
Yuji Tsutsumi, Kutsuyoshi Hamada, Tomoko Nishiguchi, Kazuhiko Fukushima, Tomoaki Nishida, Ryuichiro Kondo
67th Annual Meeting of The Botanical Society of Japan
Sapporo, Japan
高分子リグニン酸化能を有するペルオキシダーゼアイソザイムCWPO-Cはポプラ木繊維の木化に関与する

佐々木慎弥、馬場啓一、小川直人、西田友昭、堤祐司、近藤隆一郎

第45回植物生理学会年会 2004.3

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Presentation type：Oral presentation (general)

Venue：東京都立大学 Country：Japan
高分子リグニン酸化能を有するペルオキシダーゼアイソザイムCWPO-Cの局在解析

佐々木慎弥、馬場啓一、西田友昭、堤祐司、近藤隆一郎

日本木材学会 2004.8

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Presentation type：Oral presentation (general)

Venue：札幌 Country：Japan
Isolation, Expression, and Localization Profile of the Lignin Polymer Oxidizing Cationic cell-wall-peroxidase in Poplus alba. L. International conference

Yuji Tsutsumi, Shinya Sasaki, Kei'ichi Baba, Tomoaki Nishida, Ryuichiro Kondo

9th International Conference on Biotechnology in the Pulp and Paper Industry 2004.10

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Presentation type：Oral presentation (general)

Venue：Durban Country：South Africa
Characterization of a cationic cell-wall-bound peroxidase responsible for the dehydrogenative polymerization of lignin in Populus alba L. International conference

Shinya Sasaki, Kei’ichi Baba, Tomoaki Nishida, Yuji Tsutsumi, Ryuichiro Kondo

13th ISWPFC 2005.5

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Presentation type：Oral presentation (general)

Venue：Auckland Country：New Zealand
マツ材線虫病抵抗性の発現に関与するクロマツ抽出物の検索

梶原　洵子、森　康浩、大平峰子、堤　祐司、近藤　隆一郎

日本森林学会 2006.4

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Presentation type：Oral presentation (general)

Venue：東京 Country：Japan
弱病原性のマツノザイセンチュウを予め接種したマツは本当に強いのか？

森康浩、宮原文彦、堤祐司，近藤隆一郎

日本森林学会 2006.4

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Presentation type：Oral presentation (general)

Venue：東京 Country：Japan
ポプラ酸性ペルオキシダーゼ群の転写および翻訳解析

佐々木慎弥、志水元亨、割石博之、堤祐司、近藤隆一郎

日本木材学会 2006.8

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Presentation type：Oral presentation (general)

Venue：秋田市 Country：Japan
マツ材線虫病抵抗性クロマツ抽出成分の抵抗性の関与

梶原洵子、森康浩、大平峰子、斉藤香織、福島和彦、堤祐司、近藤隆一郎

日本木材学会 2006.8

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Presentation type：Oral presentation (general)

Venue：秋田市 Country：Japan
ポプラペルオキシダーゼ遺伝子の網羅的単離と転写解析

江藤　祐、佐々木慎弥、堤祐司、近藤隆一郎

日本木材学会 2006.8

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Presentation type：Oral presentation (general)

Venue：秋田市 Country：Japan
耐塩性白色腐朽菌 Phlebia sp. MG-60の産生するマンガンペルオキシダーゼ

亀井一郎、大黒千映子、堤　祐司、近藤隆一郎

日本生物工学会 2006.9

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Presentation type：Oral presentation (general)

Venue：大阪府豊中市 Country：Japan
ポプラペルオキシダーゼの網羅的解析(2) 〜転写およびプロテオーム解析〜

堤祐司，佐々木慎弥，江藤祐，近藤隆一郎

日本植物学会 2006.9

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Presentation type：Oral presentation (general)

Venue：熊本市 Country：Japan
耐塩性白色腐朽菌 Phlebia sp. MG-60の産生するマンガンペルオキシダーゼの発現解析

亀井一郎、大黒千映子、堤　祐司、近藤隆一郎

リグニン討論会 2006.10

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Presentation type：Oral presentation (general)

Venue：札幌市 Country：Japan
細胞壁結合性ペルオキシダーゼアイソザイムの触媒機構と木化における役割

佐々木慎弥、堤　祐司、近藤隆一郎

日本木材学会九州支部 2006.10

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Presentation type：Oral presentation (general)

Venue：熊本市 Country：Japan
高分子リグニン酸化能を有するペルオキシダーゼアイソザイムCWPO-Cの機能解析

佐々木慎弥、野中大輔、割石博之、堤祐司、近藤隆一郎

リグニン討論会 2006.10

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Presentation type：Oral presentation (general)

Venue：札幌市 Country：Japan
クロマツにおけるマツ材線虫病抵抗性とマツノザイセンチュウの移動との関係

森康浩、宮原文彦、堤　祐司、近藤隆一郎

日本木材学会九州支部 2006.10

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Presentation type：Oral presentation (general)

Venue：熊本市 Country：Japan
細胞壁結合性ペルオキシダーゼアイソザイムCWPO-Cの構造解析およびリグニンの酸化機構

佐々木慎弥，堤祐司，近藤隆一郎

日本植物生理学会 2007.3

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Presentation type：Oral presentation (general)

Venue：札幌市 Country：Japan
推定CWPO-CホモログAtPrx1、AtPrx 25、 AtPrx 71のリグニン生合成への関与

重藤潤, 伊藤義隆, 清永裕子, 堤祐司

第19回日本木材学会九州支部大会 2012.8

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Language：Japanese Presentation type：Oral presentation (general)

Venue：宮崎市 Country：Japan
シロイヌナズナにおけるリグニン形成特異的ペルオキシダーゼの酸化能

重藤潤, 長野万里子, 堤祐司

第54回日本植物生理学会年会 2013.3

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Language：Japanese Presentation type：Oral presentation (general)

Venue：岡山 Country：Japan
Laser microdissectionを用いたリグニンの不均一性制御機構の解明

和田卓, 藤田弘毅, 堤祐司

第63回日本木材学会大会 2013.3

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Language：Japanese Presentation type：Oral presentation (general)

Venue：盛岡 Country：Japan
テルピノレンはヒノキチオール生合成中間体である

豊丹生泰史, 黒田健一, 藤田弘毅, 堤祐司, 芦谷竜矢

第63回日本木材学会大会 2013.3

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Language：Japanese Presentation type：Oral presentation (general)

Venue：盛岡 Country：Japan
リグニン生合成に関与するシロイヌナズナペルオキシダーゼの基質酸化能の解析

長野万里子, 重藤潤, 堤祐司

第63回日本木材学会大会 2013.3

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Language：Japanese

Venue：岩手 Country：Japan
リグニン高分子化ペルオキシダーゼの反応特性

重藤潤, 長野万里子, 堤祐司

日本植物学会第77回大会 2013.9

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Language：Japanese Presentation type：Oral presentation (general)

Venue：札幌 Country：Japan

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MISC

Diverse functions and reactions of class III peroxidases

Jun Shigeto, Yuji Tsutsumi

New Phytologist 2016.3

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Language：English Publishing type：Article, review, commentary, editorial, etc. (scientific journal)

Higher plants contain plant-specific peroxidases (class III peroxidase; Prxs) that exist as large multigene families. Reverse genetic studies to characterize the function of each Prx have revealed that Prxs are involved in lignification, cell elongation, stress defense and seed germination. However, the underlying mechanisms associated with plant phenotypes following genetic engineering of Prx genes are not fully understood. This is because Prxs can function as catalytic enzymes that oxidize phenolic compounds while consuming hydrogen peroxide and/or as generators of reactive oxygen species. Moreover, biochemical efforts to characterize Prxs responsible for lignin polymerization have revealed specialized activities of Prxs. In conclusion, not only spatiotemporal regulation of gene expression and protein distribution, but also differentiated oxidation properties of each Prx define the function of this class of peroxidases.

Other Link： http://onlinelibrary.wiley.com/doi/10.1111/nph.13738/full
竹の統合バイオリファイナリーに向けて

堤祐司, 藤田弘毅, 河野　陽平, 亀井　一郎

Cellulose Communication 2017.12

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Language：Japanese Publishing type：Article, review, commentary, editorial, etc. (scientific journal)

Bamboo is a one of fast growing plants and expected to be a sutainable biomass for bio-re nery in Aisa. The bio- re nery technology for bamboo is an imperative because the environmental problem by the yearly expanding bamboo forest which has low commercial value. In our research project we attempted to develop the integrated bamboo bio-re nery. The project consists of the development of the utilization of all components of bamboo, lignin, cellulose, and hemicellulose. Bamboo itself and high molecular weight lignin is lique ed, then converted to the polyuretan form and carbon ber. The bioactive compounds such as anti-cancer activity are also screend in the low molecular weight degraded lignin fraction. In the hemicellulose utilization, homologous recombination and RNAi of fungi are achieved to modi y metabolic pathway for hemicellulose conversion. Production of microcrystalline cellulose from bamboo pulp from the medical and food additives is now ongoin. The project also consists the development of a community-based consolidating system for bamboo.
スギ抽出成分の生理活性物質としての利用

堤　祐司、西田　友昭

紙パルプの技術 2000.1

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Language：Japanese Publishing type：Article, review, commentary, editorial, etc. (scientific journal)

Utilization of the extracts from sugi (Cryptomeria japonica D.Don) as bioactive compounds

Industrial property rights

Patent	Number of applications: 6	Number of registrations: 5
Utility model	Number of applications: 0	Number of registrations: 0
Design	Number of applications: 0	Number of registrations: 0
Trademark	Number of applications: 0	Number of registrations: 0

Professional Memberships

日本木材学会
日本植物学会
日本植物生理学会
紙パルプ技術協会
リグニン学会

Committee Memberships

リグニン学会 Executive Domestic

2023.4 - 2025.3
日本木材学会 Councilor Domestic

2023.4 - 2025.3
日本木材学会 Executive Domestic

2022.4 - 2023.5
九州紙パルプ研究会 Executive Domestic

2021.4 - 2025.3
日本木材学会　九州支部 Executive Domestic

2021.4 - 2025.3
日本木材学会 Councilor Domestic

2021.4 - 2023.3
リグニン学会 Executive Domestic

2021.4 - 2022.4
九州紙パルプ研究会 Executive Domestic

2019.4 - 2021.3
日本木材学会 Councilor Domestic

2019.4 - 2021.3
日本木材学会　九州支部 Executive Domestic

2019.4 - 2021.3
リグニン学会 Executive Domestic

2018.10 - 2021.3
紙パルプ技術協会 Executive Domestic

2018.7 - 2024.6
日本木材学会 Executive Domestic

2018.4 - 2020.3
日本木材学会 Executive Domestic

2017.4 - 2019.3
日本木材学会　九州支部 Chairman Domestic

2017.4 - 2018.3
紙パルプ研究会 Executive Domestic

2016.4 - 2019.3
日本木材学会 Executive Domestic

2016.4 - 2018.3
日本木材学会 Councilor Domestic

2015.4 - 2017.3
日本木材学会　九州支部 Vice-chairman Domestic

2015.4 - 2017.3
九州紙パルプ研究会 Executive Domestic

2014.4 - 2019.3
日本木材学会 Councilor Domestic

2013.4 - 2015.3
九州紙パルプ研究会 Chairman Domestic

2012.4 - 2014.3
九州紙パルプ研究会代表幹事 Domestic

2012.4 - 2014.3
日本木材学会　九州支部常任理事（総務） Domestic

2008.4 - 2010.3

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Academic Activities

運営委員長

第73回日本木材学会大会（ Japan ） 2023.3

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Type：Competition, symposium, etc.

Number of participants：1,000
Screening of academic papers

Role(s)： Peer review

2023

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Type：Peer review

Number of peer-reviewed articles in foreign language journals：4

Number of peer-reviewed articles in Japanese journals：1

Proceedings of International Conference Number of peer-reviewed papers：0

Proceedings of domestic conference Number of peer-reviewed papers：0
Screening of academic papers

Role(s)： Peer review

2022

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Type：Peer review

Number of peer-reviewed articles in foreign language journals：5

Number of peer-reviewed articles in Japanese journals：0

Proceedings of International Conference Number of peer-reviewed papers：0

Proceedings of domestic conference Number of peer-reviewed papers：0
Screening of academic papers

Role(s)： Peer review

2022

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Type：Peer review

Number of peer-reviewed articles in foreign language journals：2

Number of peer-reviewed articles in Japanese journals：0

Proceedings of International Conference Number of peer-reviewed papers：0

Proceedings of domestic conference Number of peer-reviewed papers：0
Screening of academic papers

Role(s)： Peer review

2021

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Type：Peer review

Number of peer-reviewed articles in foreign language journals：6

Number of peer-reviewed articles in Japanese journals：0

Proceedings of International Conference Number of peer-reviewed papers：0

Proceedings of domestic conference Number of peer-reviewed papers：0
Screening of academic papers

Role(s)： Peer review

2020

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Type：Peer review

Number of peer-reviewed articles in foreign language journals：8

Number of peer-reviewed articles in Japanese journals：0

Proceedings of International Conference Number of peer-reviewed papers：0

Proceedings of domestic conference Number of peer-reviewed papers：0
Screening of academic papers

Role(s)： Peer review

2019

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Type：Peer review

Number of peer-reviewed articles in foreign language journals：6

Number of peer-reviewed articles in Japanese journals：2

Proceedings of International Conference Number of peer-reviewed papers：0

Proceedings of domestic conference Number of peer-reviewed papers：0
Screening of academic papers

Role(s)： Peer review

2018

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Type：Peer review

Number of peer-reviewed articles in foreign language journals：6

Number of peer-reviewed articles in Japanese journals：0

Proceedings of International Conference Number of peer-reviewed papers：0

Proceedings of domestic conference Number of peer-reviewed papers：0
大会実行委員長

第６７回日本木材学会大会（ Japan ） 2017.3

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Type：Competition, symposium, etc.

Number of participants：1,050
Screening of academic papers

Role(s)： Peer review

2017

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Type：Peer review

Number of peer-reviewed articles in foreign language journals：9

Number of peer-reviewed articles in Japanese journals：0

Proceedings of International Conference Number of peer-reviewed papers：0

Proceedings of domestic conference Number of peer-reviewed papers：0
Committee Member, Japan International contribution

LignoBiotech IV symposium （ Barcelona Spain ） 2016.6

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Type：Competition, symposium, etc.

Number of participants：200
Committee Member, Japan International contribution

LignoBiotech III symposium （ Chile ） 2014.7

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Type：Competition, symposium, etc.

Number of participants：200
大会総務

第５７回リグニン討論会（ Japan ） 2012.10

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Type：Competition, symposium, etc.

Number of participants：80
総務 International contribution

LignoBiotech II symposium （ Japan ） 2012.10

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Type：Competition, symposium, etc.

Number of participants：200
開催役員

第36回九州紙パルプ研究会（ Japan ） 2012.6

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Type：Competition, symposium, etc.

Number of participants：80
座長（Chairmanship）

第５６回リグニン討論会（ Japan ） 2011.9

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Type：Competition, symposium, etc.
座長（Chairmanship）

第６１回日本木材学会（ Japan ） 2011.3

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Type：Competition, symposium, etc.
総務

第61回日本木材学会（ Japan ） 2011.3

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Type：Competition, symposium, etc.
座長（Chairmanship）

第５５回リグニン討論会（ Japan ） 2010.10

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Type：Competition, symposium, etc.
座長（Chairmanship）

第５０回リグニン討論会（ Japan ） 2005.10 - Present

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Type：Competition, symposium, etc.
Local committee International contribution

The 7th International Peroxidase Symposium （ Japan ） 2005.9

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Type：Competition, symposium, etc.

Number of participants：90
座長（Chairmanship）

第５5会日本木材学会（ Japan ） 2005.3 - Present

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Type：Competition, symposium, etc.
座長（Chairmanship）

第４９回リグニン討論会（ Japan ） 2004.11 - Present

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Type：Competition, symposium, etc.
総務

第１１回　日本木材学会九州支部大会（ Japan ） 2004.10 - Present

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Type：Competition, symposium, etc.

Number of participants：120
座長（Chairmanship）

第５４会日本木材学会（ Japan ） 2004.8 - Present

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Type：Competition, symposium, etc.
座長（Chairmanship）

第４８回リグニン討論会（ Japan ） 2003.10 - Present

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Type：Competition, symposium, etc.
大会運営委員

九州紙パルプ研究会（ Japan ） 2003.6 - Present

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Type：Competition, symposium, etc.

Number of participants：100
座長（Chairmanship）

第５３会日本木材学会（ Japan ） 2003.3 - Present

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Type：Competition, symposium, etc.
大会運営委員

第５３回　日本木材学会大会（ Japan ） 2003.3 - Present

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Type：Competition, symposium, etc.

Number of participants：1,000

▼display all

Research Projects

万能ペルオキシダーゼの異なる生理機能発現；木本のリグニン形成と草本の成長制御

Grant number：24K01828 2024 - 2026

Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)

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Authorship：Principal investigator Grant type：Scientific research funding
万能ペルオキシダーゼCWPO-Cの植物ホルモン不活性化による生長・分化制御

Grant number：20H03046 2020 - 2022

Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)

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Authorship：Principal investigator Grant type：Scientific research funding
奨学寄付金

2019

　 More details

Grant type：Donation
基質万能性ペルオキシダーゼは植物ホルモン代謝を介して形態制御に関与する

Grant number：18K19234 2018 - 2019

Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Challenging Research(Exploratory)

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Authorship：Principal investigator Grant type：Scientific research funding
樹木の生長、分岐を制御する植物ペルオキシダーゼの作用機構の解明

Grant number：17H03846 2017 - 2019

Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)

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Authorship：Principal investigator Grant type：Scientific research funding
奨学寄付金

2017

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Grant type：Donation
奨学寄付金

2016

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Grant type：Donation
バンブーリファイナリー技術開発による竹林有効利用の先進的九州モデル構築

2015.5 - 2018.3

　 More details

Authorship：Principal investigator
バンブーリファイナリー技術開発による竹林有効利用の先進的九州モデル構築

2015 - 2017

農林水産業・食品産業科学技術研究推進事業シーズ創出事業

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Authorship：Principal investigator Grant type：Contract research
ポプラペルオキシダーゼCWPO-Cは木化と分化・形態形成を制御する

Grant number：15K14774 2015 - 2016

Grants-in-Aid for Scientific Research Grant-in-Aid for challenging Exploratory Research

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Authorship：Principal investigator Grant type：Scientific research funding
細胞壁へのリグニンモノマー供給を調節する輸送体・転写因子の同定⇒有用樹種開発へ

Grant number：26292097 2014 - 2016

Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)

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Authorship：Principal investigator Grant type：Scientific research funding
リグニン重合酵素のマルチノックアウトによる低リグニン・易分解性変異体植物の創出

Grant number：25660140 2013 - 2014

Grants-in-Aid for Scientific Research Grant-in-Aid for challenging Exploratory Research

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Authorship：Principal investigator Grant type：Scientific research funding
「牛肉骨粉を用いた亜臨界水処理等の低コスト不活化処理技術の開発」

2012.4 - 2013.3

Research commissions

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Authorship：Principal investigator Grant type：Other funds from industry-academia collaboration
「牛肉骨粉を用いた亜臨界水処理等の低コスト不活化処理技術の開発」

2011.4 - 2012.3

Research commissions

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Authorship：Principal investigator Grant type：Other funds from industry-academia collaboration
微細組織でのリグニン蓄積と生合成遺伝子発現解析よる詳細な木化過程の解明

Grant number：23380102 2011 - 2013

Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)

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Authorship：Principal investigator Grant type：Scientific research funding
医療器具によるプリオン病二次感染を防除する新規酵素洗浄剤の開発

Grant number：23658277 2011 - 2012

Grants-in-Aid for Scientific Research Grant-in-Aid for challenging Exploratory Research

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Authorship：Principal investigator Grant type：Scientific research funding
エネルギー生産資源としての木質バイオマスの開発

2008.12 - 2014.11

Research commissions

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Authorship：Principal investigator Grant type：Other funds from industry-academia collaboration
エネルギー生産資源としての木質バイオマスの開発

2008.12 - 2012.11

株式会社東洋高圧

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Authorship：Principal investigator

エネルギー生産に適した木質バイオマス資源となる有用樹木を開発する
鳥インフルエンザ、ＢＳＥ等の高精度かつ効率的なリスク管理技術の開発委託事業

2008.5 - 2012.3

　 More details

Authorship：Principal investigator
平成２３年度鳥インフルエンザ、ＢＳＥ、口蹄疫等の効率的なリスク低減技術の開発牛肉骨粉を用いた亜臨界水処理等の低コスト不活化処理技術の開発

2008 - 2012

（亜臨界）農林水産技術会議

　 More details

Authorship：Principal investigator Grant type：Contract research
マンガンペルオキシダーゼによる異常プリオン感染性の消去とメカニズム解明

Grant number：19658069 2007 - 2008

Grants-in-Aid for Scientific Research Grant-in-Aid for Exploratory Research

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Authorship：Principal investigator Grant type：Scientific research funding
木質系廃棄物からのバイオエタノール生産に向けた高分解能白色腐朽菌前処理の可能性評価

2006

九州大学２１世紀ＣＯＥプログラム「循環型住空間システムの構築」萌芽的学際研究助成

　 More details

Authorship：Principal investigator Grant type：Contract research
バイオレメディエーションによるプリオン不活化

2006

牛海綿状脳症（ＢＳＥ）及び人獣共通感染症の制圧のための技術開発

　 More details

Authorship：Coinvestigator(s) Grant type：Contract research
異常プリオン分解酵素による器具洗浄剤の開発

2006

産学連携戦略･次世代産業創出事業　研究開発委託事業

　 More details

Authorship：Coinvestigator(s) Grant type：Contract research
アレルゲンとなるダニ・カビの高感度マイクロアレイ検出技術の開発

2006

JSTーズ発掘試験研究申請

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Authorship：Principal investigator Grant type：Contract research
奨学寄付金

2006

　 More details

Grant type：Donation
木質バイオマスの生成・分解・機能に対する計算化学解析

Grant number：17380103 2005 - 2007

Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)

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Authorship：Coinvestigator(s) Grant type：Scientific research funding

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Educational Activities

Advanced Watershed Geo-system Science

Advanced Forest Geo-and Bio-system Science

Class subject

森林機能開発学実験

2023.10 - 2024.3 Second semester
森林化学II

2023.6 - 2023.8 Summer quarter
科学英語（森林機能開発学分野）

2023.4 - 2023.9 First semester
森林化学I

2023.4 - 2023.6 Spring quarter
基礎生物有機化学I

2023.4 - 2023.6 Spring quarter
森林機能開発学実験

2022.10 - 2023.3 Second semester
森林化学II

2022.6 - 2022.8 Summer quarter
基礎生物有機化学II

2022.6 - 2022.8 Summer quarter
科学英語（森林機能開発学分野）

2022.4 - 2022.9 First semester
森林化学I

2022.4 - 2022.6 Spring quarter
基礎生物有機化学I

2022.4 - 2022.6 Spring quarter
生体物質化学

2021.12 - 2022.2 Winter quarter
森林機能開発学実験

2021.10 - 2022.3 Second semester
森林バイオマス科学

2021.10 - 2021.12 Fall quarter
Forest Products Sience

2021.4 - 2021.9 First semester
サスティナブル資源科学特論（E科目）」

2021.4 - 2021.9 First semester
基礎生物有機化学

2021.4 - 2021.9 First semester
森林化学

2021.4 - 2021.9 First semester
科学英語（森林機能開発学分野）

2021.4 - 2021.9 First semester
木質の形成と利用

2021.4 - 2021.6 Spring quarter
生体物質化学

2020.12 - 2021.2 Winter quarter
森林機能開発学実験

2020.10 - 2021.3 Second semester
森林バイオマス科学

2020.10 - 2020.12 Fall quarter
サスティナブル資源科学特論（E科目）」

2020.4 - 2020.9 First semester
Forest Products Sience

2020.4 - 2020.9 First semester
科学英語（森林機能開発学分野）

2020.4 - 2020.9 First semester
森林化学

2020.4 - 2020.9 First semester
基礎生物有機化学

2020.4 - 2020.9 First semester
木質の形成と利用

2020.4 - 2020.6 Spring quarter
生体物質化学

2019.12 - 2020.2 Winter quarter
森林機能開発学実験

2019.10 - 2020.3 Second semester
森林バイオマス科学

2019.10 - 2019.12 Fall quarter
基礎生物有機化学

2019.4 - 2019.9 First semester
サスティナブル資源科学特論（E科目）」

2019.4 - 2019.9 First semester
森林化学

2019.4 - 2019.9 First semester
科学英語（森林機能開発学分野）

2019.4 - 2019.9 First semester
木質の形成と利用

2019.4 - 2019.6 Spring quarter
生体物質化学

2018.12 - 2019.2 Winter quarter
森林バイオマス科学

2018.10 - 2018.12 Fall quarter
基礎生物有機化学

2018.4 - 2018.9 First semester
木材化学

2018.4 - 2018.9 First semester
木質の形成と利用

2018.4 - 2018.6 Spring quarter
科学英語II

2017.10 - 2018.3 Second semester
森林機能開発学実験

2017.10 - 2018.3 Second semester
Forest Products Sience

2017.10 - 2018.3 Second semester
サスティナブル資源科学特別研究第二

2017.4 - 2018.3 Full year
サスティナブル資源科学特別講究

2017.4 - 2018.3 Full year
サスティナブル資源科学特別講究

2017.4 - 2018.3 Full year
国際演示法

2017.4 - 2018.3 Full year
サスティナブル資源科学特別研究第一

2017.4 - 2018.3 Full year
サスティナブル資源科学プロジェクト演習

2017.4 - 2018.3 Full year
基礎生物有機化学

2017.4 - 2017.9 First semester
基礎化学Ａ

2017.4 - 2017.9 First semester
木材化学

2017.4 - 2017.9 First semester
科学英語（森林機能開発学分野）

2017.4 - 2017.9 First semester
基礎生物有機化学

2017.4 - 2017.9 First semester
森林化学特論

2017.4 - 2017.9 First semester
森林化学特論

2017.4 - 2017.9 First semester
木材化学

2017.4 - 2017.9 First semester
Master's Thesis

2017.4 - 2017.6 Spring quarter
Master’s Thesis ResearchⅠ

2017.4 - 2017.6 Spring quarter
Master's Thesis Research II

2017.4 - 2017.6 Spring quarter
科学英語I

2016.10 - 2017.3 Second semester
Forest Products Sience

2016.10 - 2017.3 Second semester
サスティナブル資源科学特別研究第一

2016.4 - 2017.3 Full year
サスティナブル資源科学プロジェクト演習

2016.4 - 2017.3 Full year
サスティナブル資源科学特別実験

2016.4 - 2017.3 Full year
サスティナブル資源科学特別講究

2016.4 - 2017.3 Full year
国際演示法

2016.4 - 2017.3 Full year
木材化学

2016.4 - 2016.9 First semester
科学英語II

2016.4 - 2016.9 First semester
森林化学特論

2016.4 - 2016.9 First semester
基礎生物有機化学

2016.4 - 2016.9 First semester
環境生物学

2016.4 - 2016.9 First semester
Forest Products Sience

2015.10 - 2016.3 Second semester
科学英語I

2015.10 - 2016.3 Second semester
サスティナブル資源科学特別演習

2015.4 - 2016.3 Full year
サスティナブル資源科学特別研究第一

2015.4 - 2016.3 Full year
国際演示法

2015.4 - 2016.3 Full year
サスティナブル資源科学特別講究

2015.4 - 2016.3 Full year
サスティナブル資源科学特別実験

2015.4 - 2016.3 Full year
サスティナブル資源科学プロジェクト演習

2015.4 - 2016.3 Full year
サスティナブル資源科学特別研究第二

2015.4 - 2016.3 Full year
森林化学特論

2015.4 - 2015.9 First semester
基礎生物有機化学

2015.4 - 2015.9 First semester
環境生物学

2015.4 - 2015.9 First semester
木材化学

2015.4 - 2015.9 First semester
科学英語I

2014.10 - 2015.3 Second semester
Forest Products Sience

2014.10 - 2015.3 Second semester
国際演示法

2014.4 - 2015.3 Full year
サスティナブル資源科学特別演習

2014.4 - 2015.3 Full year
サスティナブル資源科学特別研究第二

2014.4 - 2015.3 Full year
サスティナブル資源科学プロジェクト演習

2014.4 - 2015.3 Full year
サスティナブル資源科学特別実験

2014.4 - 2015.3 Full year
サスティナブル資源科学特別研究第一

2014.4 - 2015.3 Full year
サスティナブル資源科学特別講究

2014.4 - 2015.3 Full year
森林生物化学特論

2014.4 - 2014.9 First semester
森林生物化学特論

2014.4 - 2014.9 First semester
木材化学

2014.4 - 2014.9 First semester
環境生物学

2014.4 - 2014.9 First semester
基礎生物有機化学

2014.4 - 2014.9 First semester
科学英語II

2014.4 - 2014.9 First semester
森林バイオマス循環

2013.10 - 2014.3 Second semester
科学英語I

2013.10 - 2014.3 Second semester
サスティナブル資源科学プロジェクト演習

2013.4 - 2014.3 Full year
国際演示法

2013.4 - 2014.3 Full year
サスティナブル資源科学特別講究

2013.4 - 2014.3 Full year
サスティナブル資源科学特別実験

2013.4 - 2014.3 Full year
サスティナブル資源科学特別演習

2013.4 - 2014.3 Full year
サスティナブル資源科学プロジェクト演習

2013.4 - 2014.3 Full year
サスティナブル資源科学特別研究第一

2013.4 - 2014.3 Full year
サスティナブル資源科学特別研究第一

2013.4 - 2014.3 Full year
サスティナブル資源科学プロジェクト演習

2013.4 - 2014.3 Full year
森林バイオテクノロジー特論

2013.4 - 2013.9 First semester
森林生物化学特論

2013.4 - 2013.9 First semester
森林生物化学特論

2013.4 - 2013.9 First semester
科学英語II

2013.4 - 2013.9 First semester
サスティナブル資源科学特別研究第一

2012.4 - 2013.3 Full year
サスティナブル資源科学プロジェクト演習

2012.4 - 2013.3 Full year
サスティナブル資源科学特別研究第二

2012.4 - 2013.3 Full year
森林生物化学特論

2012.4 - 2012.9 First semester
森林生物化学特論

2012.4 - 2012.9 First semester
基礎有機化学実験

2011.10 - 2012.3 Second semester
森林バイオマス循環

2011.10 - 2012.3 Second semester
有機化学

2011.10 - 2012.3 Second semester
森林圏生物成分機能学

2011.10 - 2012.3 Second semester
サスティナブル資源科学プロジェクト演習

2011.4 - 2012.3 Full year
森林圏環境資源科学特別研究第二

2011.4 - 2012.3 Full year
サスティナブル資源科学特別研究第一

2011.4 - 2012.3 Full year
森林圏環境資源科学演習第二

2011.4 - 2012.3 Full year
環境生物学

2011.4 - 2011.9 First semester
森林圏環境資源科学特論

2011.4 - 2011.9 First semester
森林バイオテクノロジー特論

2011.4 - 2011.9 First semester
森林バイオマス循環

2010.10 - 2011.3 Second semester
基礎有機化学実験

2010.10 - 2011.3 Second semester
森林圏生物成分機能学

2010.10 - 2011.3 Second semester
有機化学

2010.10 - 2011.3 Second semester
森林圏環境資源科学特別研究第二

2010.4 - 2011.3 Full year
サスティナブル資源科学プロジェクト演習

2010.4 - 2011.3 Full year
森林圏環境資源科学演習第二

2010.4 - 2011.3 Full year
サスティナブル資源科学特別研究第一

2010.4 - 2011.3 Full year
森林圏環境資源科学特論

2010.4 - 2010.9 First semester
環境生物学

2010.4 - 2010.9 First semester
森林バイオテクノロジー特論

2010.4 - 2010.9 First semester
基礎有機化学実験

2007.10 - 2008.3 Second semester
有機化学

2007.10 - 2008.3 Second semester
森林圏生物成分機能学

2007.10 - 2008.3 Second semester
森林圏環境資源科学演習第一

2007.4 - 2008.3 Full year
森林圏環境資源科学特別研究第二

2007.4 - 2008.3 Full year
森林圏環境資源科学特別研究第一

2007.4 - 2008.3 Full year
森林圏環境資源科学演習第二

2007.4 - 2008.3 Full year
環境生物学

2007.4 - 2007.9 First semester
森林圏環境科学特論

2007.4 - 2007.9 First semester
森林圏環境資源科学特論

2007.4 - 2007.9 First semester
有機化学

2006.10 - 2007.3 Second semester
基礎有機化学実験

2006.10 - 2007.3 Second semester
森林圏生物成分機能学

2006.10 - 2007.3 Second semester
森林圏環境科学特論

2006.4 - 2006.9 First semester
環境生物学

2006.4 - 2006.9 First semester
森林圏環境資源科学特論

2006.4 - 2006.9 First semester
森林圏環境資源科学特論

2005.4 - 2005.9 First semester
森林圏環境科学特論

2005.4 - 2005.9 First semester
森林圏環境資源科学特論

2004.4 - 2004.9 First semester
森林圏環境科学特論

2004.4 - 2004.9 First semester
森林圏環境科学特論

2003.4 - 2003.9 First semester
森林圏環境資源科学特論

2003.4 - 2003.9 First semester
森林圏環境科学特論

2002.4 - 2002.9 First semester
森林圏環境資源科学特論

2002.4 - 2002.9 First semester

▼display all

FD Participation

2023.10 Role：Participation Title：遺伝子組換え実験の安全管理について

Organizer：[Undergraduate school/graduate school/graduate faculty]
2022.3 Role：Participation Title：国費特別プログラム「未来の農を描くビジョンデザイン実践プログラム」実施に向けて

Organizer：[Undergraduate school/graduate school/graduate faculty]
2021.11 Role：Participation Title：農学研究院FD「人を対象とする生命科学・医学系研究に関する倫理指針」

Organizer：[Undergraduate school/graduate school/graduate faculty]
2019.11 Role：Participation Title：留学生との共生に向けて

Organizer：[Undergraduate school/graduate school/graduate faculty]
2019.5 Role：Participation Title：優良な博士人材の獲得と育成に向けて～農学研究院教授に学ぶ～

Organizer：[Undergraduate school/graduate school/graduate faculty]
2018.10 Role：Participation Title：M2B学習支援システム講習会

Organizer：University-wide
2018.3 Role：Participation Title：第５回農学研究院ＦＤ　m2b（ミツバ）システムの授業への活用

Organizer：[Undergraduate school/graduate school/graduate faculty]
2017.11 Role：Participation Title：産学官共同研究における費用負担と営業秘密の管理について

Organizer：[Undergraduate school/graduate school/graduate faculty]
2017.9 Role：Participation Title：研究分析ツール「SciVal」及び研究者プロファイリングツール「Pure」に関する説明会（応用編）

Organizer：[Undergraduate school/graduate school/graduate faculty]
2017.9 Role：Participation Title：第３回農学研究院ＦＤ研究分析ツール「SciVal」及び研究者プロファイリングツール「Pure」　に関する説明会（応用編）

Organizer：[Undergraduate school/graduate school/graduate faculty]
2016.11 Role：Participation Title：韓国のサバティカルの実情について

Organizer：[Undergraduate school/graduate school/graduate faculty]
2015.9 Role：Participation Title：ハラスメントの実際と対応

Organizer：[Undergraduate school/graduate school/graduate faculty]
2014.7 Role：Participation Title：グローバル人材育成推進事業－教員の英語スキルアッププログラムについて－

Organizer：[Undergraduate school/graduate school/graduate faculty]
2014.7 Role：Participation Title：ハラスメント防止の要点

Organizer：[Undergraduate school/graduate school/graduate faculty]
2014.5 Role：Participation Title：特許セミナー～大学における研究成果を知的財産として活用するために～

Organizer：[Undergraduate school/graduate school/graduate faculty]
2013.11 Role：Participation Title：「持続可能な社会を開く決断科学大学院プログラム」

Organizer：[Undergraduate school/graduate school/graduate faculty]
2013.7 Role：Participation Title：基幹教育と基幹教育カリキュラム

Organizer：[Undergraduate school/graduate school/graduate faculty]
2011.7 Role：Participation Title：こころが不調になった学生の理解と接し方－メンタルヘルスの基礎知識

Organizer：[Undergraduate school/graduate school/graduate faculty]
2011.3 Role：Participation Title：平成22年よりスタートした新プログラムの年度報告及びIPAAE関係報告会

Organizer：[Undergraduate school/graduate school/graduate faculty]

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Visiting, concurrent, or part-time lecturers at other universities, institutions, etc.

2024 静岡大学総合科学技術研究科 Classification:Intensive course Domestic/International Classification:Japan

Semester, Day Time or Duration：前期　１単位
2018 京都大学大学院森林科学専攻大学院特別講義 Classification:Intensive course Domestic/International Classification:Japan

Semester, Day Time or Duration：2018年１２月17-18日　１単位　２日間
2016 静岡大学　農学部 Classification:Intensive course Domestic/International Classification:Japan

Semester, Day Time or Duration：８月６日〜９日
2014 放送大学 Classification:Part-time lecturer Domestic/International Classification:Japan

Semester, Day Time or Duration：後期、集中

Other educational activity and Special note

2017 Class Teacher 学部
2013 Class Teacher 学部

Outline of Social Contribution and International Cooperation activities

Editor of Journal of Wood Science (The Japan Wood Reseach Society)
Scientific Committee of LignoBiotech symposium (2012-2018)