出版者・発行元：Cytotechnology  

Sea cucumbers are both versatile marine organisms and an Asian marine food known to have several medicinal effects. We evaluated the anti-allergic potential of some major purified holostane-type saponins from the body wall of the black sea cucumber, Holothuria atra. Six saponin compounds were isolated, holothurin B (1), holothurin A (2), 24-dehydro echinoside A (3), desholothurin A1 (4), desholothurin A (5), and des 24-dehydro echinoside A (6). The structures were identified based on spectroscopic methods and by comparison with the literature. Each compound’s inhibitory activity toward the release of β-hexosaminidase was evaluated. Among the six compounds, holothurin B (1) showed the strongest inhibition of the degranulation at all tested concentrations in a dose-dependent manner, compared to the positive control, quercetin. We also observed that holothurin B (1) was able to alleviate the inflammatory mediators interleukin (IL)-6, IL-13, and tumor necrosis factor-alpha (TNF-α). Holothurin B (1) also inhibited the Ca2+ influx stimulated by the calcium ionophore A23187, by suppressing the expression of inositol-1,4,5-triphosphate receptor (IP3R) mRNA. These results suggest that (i) holothurin B (1) has good anti-allergy activity without cytotoxicity at effective concentrations, and (ii) this compound could be a lead compound for the treatment of allergic diseases and associated inflammation. We also performed a molecular docking study for the tested compounds to correlate their binding modes and affinity for the IP3R with the in vitro results. The results concluded that the holostane-type saponins could be used as anti-allergy agents, which may be attributed to their holostane group.

DOI： 10.1007/s10616-024-00649-8

Web of Science

Scopus

記述言語：英語   出版者・発行元：Journal of Agricultural and Food Chemistry  

Flammulina velutipes (enokitake) is widely recognized for its nutritional and medicinal properties. Understanding the biochemical processes, such as lipid metabolism during fruiting body formation, is essential for enhancing mushroom cultivation and utilization. This study aimed at elucidating the dynamic lipidomic changes during seven growth stages of F. velutipes using ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry. Our results revealed significant increases in ceramides along with the growth and a sharp decline in phosphatidylinositols from mycelial to primordial stages. Fatty acid esters of hydroxy fatty acids, recently discovered for their bioactivities, showed high intensities in the mycelial and primordial stages but decreased rapidly thereafter. These findings provide profound insights into the lipid profiles associated with mushroom morphology and development. This lipidomics study establishes a foundational understanding for future research in agricultural and food chemistry applications, potentially improving industrial production and quality control of F. velutipes.

DOI： 10.1021/acs.jafc.4c03863

Web of Science

Scopus

PubMed

記述言語：英語  

記述言語：英語   出版者・発行元：Metabolomics  

Introduction: Microbial communities affect several aspects of the earth’s ecosystem through their metabolic interaction. The dynamics of this interaction emerge from complex multilevel networks of crosstalk. Elucidation of this interaction could help us to maintain the balance for a sustainable future. Objectives: To investigate the chemical language among highly abundant microbial genera in the rhizospheres of medicinal plants based on the metabolomic analysis at the interaction level. Methods: Coculturing experiments involving three microbial species: Aspergillus (A), Trichoderma (T), and Bacillus (B), representing fungi (A, T) and bacteria (B), respectively. These experiments encompassed various interaction levels, including dual cultures (AB, AT, TB) and triple cultures (ATB). Metabolic profiling by LC-QTOFMS revealed the effect of interaction level on the productivity and diversity of microbial specialized metabolites. Results: The ATB interaction had the richest profile, while the bacterial profile in the monoculture condition had the lowest. Two native compounds of the Aspergillus genus, aspergillic acid and the dipeptide asperopiperazine B, exhibited decreased levels in the presence of the AT interaction and were undetectable in the presence of bacteria during the interaction. Trichodermarin N and Trichodermatide D isolated from Trichoderma species exclusively detected during coexistence with bacteria (TB and ATB). These findings indicate that the presence of Bacillus activates cryptic biosynthetic gene clusters in Trichoderma. The antibacterial activity of mixed culture extracts was stronger than that of the monoculture extracts. The TB extract exhibited strong antifungal activity compared to the monoculture extract and other mixed culture treatments. Conclusion: The elucidation of medicinal plant microbiome interaction chemistry and its effect on the environment will also be of great interest in the context of medicinal plant health Additionally, it sheds light on the content of bioactive constituents, and facilitating the discovery of novel antimicrobials.

DOI： 10.1007/s11306-024-02138-7

Web of Science

Scopus

PubMed

記述言語：英語   出版者・発行元：Inflammopharmacology  

Parkinsonism is an age-related neurodegenerative illness that affects motor coordination leading to loss of dopaminergic neurons. Many medications are used for the treatment of Parkinson’s disease but are only symptomatic and have a limited effect on the progression of this ailment. Therefore, bioactive compounds which derived from plants have been examined for their ability to improve the neuronal damage and cell death happened in parkinsonian patients. In this study the iridoids-rich fraction isolated from Pentas lanceolata (PIRF) leaves was investigated for its phytoconstituents. Seven iridoids (1–7) and one flavonol diglycoside (8) were isolated, and their chemical structures were achieved by 1H and 13C nuclear magnetic resonance and ESI–MS spectral data. Compound 1 (6β,7β-epoxy-8-epi-splendoside) and 5 (gaertneroside) were isolated for the first time from Pentas genus as well as compound 8 (kaempferol-3-O-robinobioside). The current study aims to investigate the possible anti-parkinsonian effect of PIRF using a rotenone model of Parkinsonism in mice. Behavioural tests (wirehanging, stair and wooden-walking tests) were done to examine the motor coordination in mice after treatment. Biochemical and histopathological examinations for brain striatum in different groups were also evaluated. Results revealed that rotenone-treated mice had poor motor functions described by depletion of dopamine and Ach levels, a significant increase in proinflammatory cytokines, IL-1B, TNF-α and Mcp-1 and oxidative biomarkers with subsequent reduction in antioxidant mediators. Disorganization of striatum, degenerated neurocytes, slight vacuolation, shrunken neurons with pyknotic nuclei and apoptotic cells are displayed by histopathological examinations. Treatment with PIRF ameliorates the neurodegeneration-induced by rotenone in the brain of mice. The anti-parkinsonian effect of PIRF could be attributed to their bioactive constituents of iridoids.

DOI： 10.1007/s10787-024-01509-9

Web of Science

Scopus

PubMed

出版者・発行元：Records of Natural Products  

Four new polyoxygenated cucurbitane triterpene glycosides named kedrojiftene A-D (4-7), were isolated via different chromatographic techniques along with three phenolic compounds (1-3) from the crude extract of Kedrostis gijef. The isolated compounds were elucidated based on 1D, 2D-NMR spectroscopic analyses, HRESIMS and comparison with the related metabolites in literature. In bioassay, the α-glucosidase inhibitory assay for the isolated compounds was carried out and compounds (1-3) showed significant inhibitory activities.

DOI： 10.25135/rnp.468.2405.3236

Web of Science

Scopus

記述言語：英語   出版者・発行元：Phytomedicine  

Background: Pulmonary Fibrosis (PF) is a progressive lung disease characterized by the diffuse interstitial tissue, leading to severe breathing difficulties. The existing treatment methods are primarily aimed at slowing the progression of the disease, underscoring the urgent need to discover new drug interventions targeting novel sites. The "gut-lung axis" represents a complex bidirectional communication system where the gut microbiota not only influences lung immunity but also responds to lung-derived signals. Recent advances have uncovered that alterations in gut microbiota composition can significantly impact respiratory diseases, offering new insights into their pathogenesis and potential therapeutic approaches. Methods: This study is based on the fundamental concepts of the lung-gut axis and our previous research, further exploring the potential mechanisms of 20(S)-Protopanaxadiol (PPD) in ginseng against PF. We utilized a bleomycin-induced mouse model of PF and employed metabolomics and 16S rRNA sequencing to investigate the pathways through which PPD regulates the pulmonary fibrosis process via the gut-lung axis. Finally, we employed strategies such as antibiotic-induced microbiota disruption and fecal microbiota transplantation (FMT) to provide a comprehensive perspective on how PPD regulates pulmonary fibrosis through gut microbiota. Results: The results of the bleomycin (BLM) mouse model of PF proved that PPD can directly act on the glycolysis- related metabolic reprogramming process in lung and the AMPK/STING pathway to improve PF. Combined the analysis of gut microbiota and related metabolites, we found that PPD can regulate the process of PF through the gut-lung axis target points G6PD and SPHK1. FMT and antibiotic-induced microbiota disruption further confirmed intermediate effect of gut microbiota in PF process and the treatment of PPD. Our study suggests that PPD can alleviate the process of pulmonary fibrosis either by directly acting on the lungs or by regulating the gut microbiota. Conclusion: This study positions PPD as a vanguard in the therapeutic landscape for pulmonary fibrosis, offering a dual mechanism of action that encompasses both modulation of gut microbiota and direct intervention at molecular targets. These insights highlight the immense therapeutic potential of harnessing the gut-lung axis.

DOI： 10.1016/j.phymed.2024.155699

Web of Science

Scopus

PubMed

記述言語：英語   出版者・発行元：Natural Product Research  

Two new cucurbitane-type triterpenoids (2,3), together with two known compounds (1,4), were isolated from the aerial parts of Kedrostis gijef. The structure of all compounds was elucidated based on NMR, HRESIMS analyses, and by comparison with the literature. Additionally, the cytotoxic activity against HeLa, Caco-2, and SH-SY5Y cell lines was determined using MTT colorimetric assay.

DOI： 10.1080/14786419.2024.2367236

Web of Science

Scopus

PubMed

記述言語：英語   出版者・発行元：Phytomedicine  

Background: Pulmonary fibrosis (PF) is an escalating global health issue, characterized by rising rates of morbidity and mortality annually. Consequently, further investigation of potential damage mechanisms and potential preventive strategies for PF are warranted. Specnuezhenide (SPN), a prominent secoiridoid compound derived from Ligustrum lucidum Ait, exhibits anti-inflammatory and anti-oxidative capacities, indicating the potential therapeutic actions on PF. However, the underlying mechanisms of SPN on PF remain unclear. Purpose: This work was aimed at investigating the protective actions of SPN on PF and the potential mechanism. Methods: In vivo, mice were administrated with bleomycin (BLM) to establish PF model. PF mice were treated with SPN (45/90 mg/kg) by gavage. In vitro, we employed TGF-β1 (10 ng/mL)-induced MLE-12 and PLFs cells, which then were treated with SPN (5, 10, 20 µM). DARTS assay, biofilm interference experiment and molecular docking were performed to investigate the molecular target of SPN. Results: In vivo, we found SPN treatment improved survival rate, alleviated pathological changes through reducing BLM-induced extracellular matrix (ECM) deposition, as well as BLM-induced epithelial-mesenchymal transition (EMT). In vitro, SPN inhibited EMT and lung fibroblast transdifferentiation. Mechanistically, SPN activated the AMPK protein to decrease the abnormally high level of PD-L1. Furthermore, the compound C, known as an AMPK inhibitor, exhibited a significant hindrance to the inhibition of SPN on TGF-β1-caused fibroblast transdifferentiation and proliferation. This outcome could be attributed to the fact that compound C could eliminate the inhibitory effects of SPN on PD-L1 expression. Interestingly, DARTS assay, biofilm interference experiment and molecular docking results all indicated that SPN could bind to AMPK, which suggested that SPN might be a potential agonist targeting AMPK protein. Conclusion: Altogether, the results in our work illustrated that SPN promoted AMPK-dependent reduction of PD-L1 protein, contributing to the inhibition of fibrosis progression. Thus, SPN may represent a potential AMPK agonist for PF treatment.

DOI： 10.1016/j.phymed.2023.155318

Web of Science

Scopus

PubMed

出版者・発行元：Phytochemistry Letters  

Megastigmanes are structurally-diverse molecules that are widely distributed in nature and occurring mainly in plant kingdom. They are oxidative degradation products from β-carotenoids with C13 skeleton and their diversity has been attributed to different functionalization at the carbon atoms, as well as glycosylation pattern. The object of this review is to summarize the published literature on megastigmane glycosides regarding their sources, 13C NMR data and biological activities. This review covers the literature published between the first report that appeared in 1968 till May 2022. It combines about 357 megastigmane glycosides with their classification, sources, biological activities, and 13C NMR data. The biological activities of megastigmane glycosides have been reported, including anti-melanogenic, anti-inflammatory, neuroprotective, antioxidant, antitumor, antimicrobial, and hepatoprotective. Anti-melanogenic and hepatoprotective activities are the most promising reported bioactivities. Besides, biosynthetic pathways as well as chemotaxonomic significance are also discussed. The present review is expected to provide a comprehensive guide on this particular class of natural products.

DOI： 10.1016/j.phytol.2024.01.008

Web of Science

Scopus

出版者・発行元：Food Bioscience  

This study investigated the inhibitory effects and mechanisms of strawberry fruit extracts (SFE) and leaf extracts (SLE) on advanced glycation end products (AGEs) formation. The results revealed that SLE exhibited a higher inhibitory efficiency compared to SFE on AGEs formation in bovine serum albumin (BSA)-fructose, -methylglyoxal (MGO) and -glyoxal (GO) models. Additionally, SLE displayed remarkable trapping capabilities of MGO and GO, surpassing those of SFE. Both SFE and SLE were found to combine with BSA for static quenching, SLE showed a stronger binding force. Moreover, SLE outperformed SFE in terms of protecting the secondary structure of proteins and preventing protein cross-linking. Finally, the high-resolution mass spectrometry analysis revealed that SLE could reduce glycation sites and significantly improve the degree of glycation. Our study suggests that SLE may serve as a superior inhibitor of glycation compared to SFE, thereby providing new insights into its potential applications.

DOI： 10.1016/j.fbio.2024.103691

Web of Science

Scopus

記述言語：英語   出版者・発行元：Food Chemistry: X  

Numerous health hazards have been connected to advanced glycation end products (AGEs). In this investigation, using reaction models including BSA-fructose, BSA- methylglyoxal (MGO), and BSA-glyoxal (GO), we examined the anti-glycation potential of eight different berry species on AGEs formation. Our results indicate that black chokeberry (Aronia melanocarpa) exhibited the highest inhibitory effects, with IC50 values of 0.35 ± 0.02, 0.45 ± 0.03, and 0.48 ± 0.11 mg/mL, respectively. Furthermore, our findings suggest that black chokeberry inhibits AGE formation by binding to BSA, which alleviates the conformation alteration, prevents protein cross-linking, and traps reactive α-dicarbonyls to form adducts. Notably, three major polyphenols, including cyanidin-3-O-galactoside, cyanidin-3-O-arabinoside, and procyanidin B2 from black chokeberry, showed remarkably inhibitory effect on MGO/GO capture, and new adducts formation was verified through LC-MS/MS analysis. In summary, our research provides a theoretical basis for the use of berries, particularly black chokeberry, as natural functional food components with potential anti-glycation effects.

DOI： 10.1016/j.fochx.2023.101032

Web of Science

Scopus

PubMed

記述言語：英語   出版者・発行元：Fitoterapia  

The eelgrass Zostera marina L. has several economic roles, from its earlier usage in the insulation industry to protecting the earth from global warming. In this study, we aimed to discover the cosmetic potential of Z. marina. A methanolic extract of Z. marina showed anti-phototoxicity and anti-melanogenesis activity with an IC50 of 17.5 μM, followed by a phytochemical analysis of its phenolic constituents. Ten compounds (1−10) were isolated by several chromatographic techniques and identified by means of nuclear magnetic resonance spectroscopy (NMR) as well as high-resolution mass spectrometry (HR/MS). The identified compounds are caffeic acid (1), 3,4-dihydroxybenzoic acid (protocatechuic acid) (2), luteolin (3), diosmetin (4), 4-coumaroyl-4′-hydroxyl phenyllactic acid (5), rosmarinic acid (6), caffeoyl-4′-hydroxy-phenyllactic acid (isorinic acid) (7), apigenin 7-O-β-D-glucopyranoside (8), luteolin 7-O-β-D-glucopyranoside (9), and luteolin 7-sulfate (10). This is the first report to identify compounds 5 and 7 from the family Zosteraceae. The isolated compounds were assessed for their anti-aging abilities and were found to exhibit good anti-phototoxicity and anti-melanogenesis activities by increasing the viability of UVB-irradiated HaCaT cells by 6% to 34% and by inhibiting melanin synthesis in B16 melanoma cells by 44% to 65%.

DOI： 10.1016/j.fitote.2023.105817

Web of Science

Scopus

PubMed

記述言語：英語   出版者・発行元：Molecules  

DOI： 10.3390/molecules29051008

Web of Science

Scopus

PubMed

記述言語：英語   掲載種別：研究論文（学術雑誌）  

記述言語：日本語   出版者・発行元：(株)医事出版社  

ブタプラセンタエキス100mgを含有するサプリメント(2錠/1日)の4週間摂取が心理的ストレス症状に与える効果を検証するため、35歳以上60歳未満の女性40名を対象に無作為化二重盲検並行群間比較試験を行った。心理的ストレス反応測定尺度(SRS-18)において、プラセンタサプリメント摂取群では特に「不機嫌・怒り」の反応に多くの改善効果が認められたが、「抑うつ・不安」「無気力」の反応には明確な改善効果は示されなかった。今回、より幅広い年齢層および多様な心理ストレス症状を有する対象者にブタプラセンタエキスの摂取効果が示され、プラセンタエキス摂取群で示された「ストレス場面における心理ストレス反応」の軽減結果は、脳由来神経栄養因子産生促進効果やコルチゾール調整機能が関与した可能性が示された。

記述言語：英語   出版者・発行元：Toxicon  

Three prenylated flavonoids (1–3) were isolated from Tetragonula biroi propolis. The structures of the isolated compounds were characterized by NMR, IR, and UV spectroscopic and mass spectrometric analyses. The cytotoxicity activity of the crude extracts, fractions and the isolated compounds were established against four cell lines such as Caco-2, HeLa, MCF-7, and OVK-18. Among the tested compounds, compound 1 showed cytotoxicity activity against MCF-7 cell lines, whereas compound 2 showed good activity against Caco-2 and OVK-18 cell lines with IC50 values of 14.73 and 14.44, respectively. Moreover, compound 3 exhibited strong activity against OVK-18 cell lines. These findings contribute to the phytochemical understanding of the T. biroi propolis, and their cytotoxicity effects for future pharmaceutical purposes.

DOI： 10.1016/j.toxicon.2023.107352

Web of Science

Scopus

PubMed

記述言語：英語   出版者・発行元：Animal Science Journal  

The purpose of this study was to investigate the time-dependent change in Reishi (Ganoderma lingzhi) triterpenoids in rumen fluid. G. lingzhi fruiting bodies were milled and incubated in a tube with rumen fluid for 0, 4, 8, 12, 24, and 48 h at 39°C. After incubation, all the tubes were freeze-dried and extracted by ethanol. The contents of 18 triterpenoids in the ethanol extract were quantitated by liquid chromatography-mass spectrometry (LC-MS/MS). Based on the results, triterpenoids were categorized into three groups: (1) rapid decrease, indicating reductions of more than 50% within 8 h; (2) mild decrease, with reductions of more than 50% within 48 h; and (3) minimal change, even after 48 h, there was not much change. Ganoderic acid C6, DM, H, K, and TR as well as Ganoderenic acid D were classified in (1); Ganoderic acid LM2 and T-Q as well as Ganoderiol F in (2); and Ganoderic acid A, B, C1, C2, I, and TN; Gnoderenic acid C; and Ganodermanontriol in (3). In addition, a relationship between chemical structure and metabolic speed was observed in some cases. The results of this study revealed that G. lingzhi triterpenoids are digested and metabolized at different speeds in ruminant fluid.

DOI： 10.1111/asj.13957

Web of Science

Scopus

PubMed

出版者・発行元：Functional Foods in Health and Disease  

Background: Sorghum is consumed in Africa, North and South America, India, and other parts of the world, but it is one of several millets in Japan, and its physiological function for Japanese is not well known. In this study, we examined the effect of sorghum on postprandial blood glucose levels in healthy Japanese. Objective: This study aims to investigate the effect of sorghum intake on postprandial blood glucose in healthy adults. Methods: A double-blind crossover study was done on 17 healthy adult men and women recruited from the general population. After an early morning fasting blood glucose test, a placebo or sorghum porridge was administered and blood glucose levels were measured at 30, 60, and 120 minutes after the first washout period. Result: At 60 and 120 minutes after intake, the blood glucose level of people who were administered sorghum porridge was significantly lower than that of those who were administered the placebo. The area under the blood glucose curve was also significantly lower for the test product than for the placebo. Conclusion: Sorghum consumption can suppress the increase in postprandial blood glucose, suggesting that sorghum is useful in preventing the development of diabetes mellitus.

DOI： 10.31989/ffhd.v14i1.1266

Web of Science

Scopus

出版者・発行元：Food Science and Human Wellness  

In the face of increasingly serious environmental pollution, the health of human lung tissues is also facing serious threats. Mogroside IIE (M2E) is the main metabolite of sweetening agents mogrosides from the anti-tussive Chinese herbal Siraitia grosvenori. The study elucidated the anti-inflammatory action and molecular mechanism of M2E against acute lung injury (ALI). A lipopolysaccharide (LPS)-induced ALI model was established in mice and MH-S cells were employed to explore the protective mechanism of M2E through the western blotting, co-immunoprecipitation, and quantitative real time-PCR analysis. The results indicated that M2E alleviated LPS-induced lung injury through restraining the activation of secreted phospholipase A2 type IIA (Pla2g2a)-epidermal growth factor receptor (EGFR). The interaction of Pla2g2a and EGFR was identified by co-immunoprecipitation. In addition, M2E protected ALI induced with LPS against inflammatory and damage which were significantly dependent upon the downregulation of AKT and mTOR via the inhibition of Pla2g2a-EGFR. Pla2g2a may represent a potential target for M2E in the improvement of LPS-induced lung injury which may represent a promising strategy to treat ALI.

DOI： 10.26599/FSHW.2022.9250025

Web of Science

Scopus

記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Journal of Wood Science  

Odor environments in living spaces can influence human physiological and psychological states. To elucidate the effect of volatile organic compounds (VOCs) of wood in living spaces on the modulations of cognitive processing, we built two experimental huts to simulate wooden construction living spaces. One hut was made of cedarwood (Cryptomeria japonica), and the other was made of resin. We used cedarwood and resin, because they are often used as construction materials in Japan. In both huts, we measured the participants’ first positivity in the early visual cortex (P1), the third positivity elicited by voluntary attention (P3b), and the automatic occipital negativity to infrequent visual stimuli in event-related potentials (ERPs), while they performed a visual discrimination task. The VOCs in both huts were measured by gas chromatography–mass spectrometry. The concentration of volatile sesquiterpenes was significantly increased in the cedarwood hut. Neither P1 nor P3b was affected by the cedarwood hut. Compared to the resin hut, we observed significantly larger occipital negativities to the infrequent stimuli in the cedarwood hut in the time windows of 280–300 ms at the mid-occipital region. These findings suggest that the increased concentration of volatile sesquiterpenes emitted from cedarwood facilitates the human brain’s response to changes in visual stimuli.

DOI： 10.1186/s10086-023-02083-4

Web of Science

Scopus

researchmap

記述言語：英語   出版者・発行元：Journal of Chromatography A  

An eco-friendly dispersive liquid-liquid microextraction mediated with a reverse micelle and coupled to an HPLC-DAD was developed for the simultaneous determination of venlafaxine and agomelatine in dosage forms and human plasma. All the parameters affecting the extraction efficiencies of both drugs were investigated and optimized. Under the optimal conditions, an effective analytes’ preconcentration with enrichment factors (EFs) up to 72 was achieved. The linearity of the method was established over the concentration range of 0.50−70.0 and 3.0−100.0 ng/mL for venlafaxine and agomelatine, respectively with good correlation coefficients > 0.998. The method exhibited low detection limits in the range of 0.15-0.89 ng/mL and excellent precisions expressed in %RSD < 3% with average recoveries between 95.0 to 101.0%. The proposed method was employed to analyze the targeted analytes in dosage forms and human plasma samples with favorable characteristics like excellent enrichment, high sensitivity, great accuracy, and high precision. Finally, the greenness of the developed method was assessed using three distinct metric tools, confirming the greenness of the proposed method. The findings of this research could have more general implications for the extraction of other analytes from various matrices.

DOI： 10.1016/j.chroma.2023.464441

Web of Science

Scopus

PubMed

記述言語：英語   出版者・発行元：Scientific Reports  

Loquat (Eriobotrya japonica) leaves contain many bioactive components such as ursolic acid (UA) and amygdalin. We investigated the effects of loquat leaf powder and methanol extract in human neuroglioma H4 cells stably expressing the Swedish-type APP695 (APPNL-H4 cells) and C57BL/6 J mice. Surprisingly, the extract greatly enhanced cellular amyloid-beta peptide (Aβ) 42 productions in APPNL-H4 cells. Administration of leaf powder increased Aβ42 levels after 3 months and decreased levels after 12 months compared to control mice. Leaf powder had no effect on working memory after 3 months, but improved working memory after 12 months. Administration of UA decreased Aβ42 and P-tau levels and improved working memory after 12 months, similar to the administration of leave powder for 12 months. Amygdalin enhanced cellular Aβ42 production in APPNL-H4 cells, which was the same as the extract. Three-month administration of amygdalin increased Aβ42 levels slightly but did not significantly increase them, which is similar to the trend observed with the administration of leaf powder for 3 months. UA was likely the main compound contained in loquat leaves responsible for the decrease in intracerebral Aβ42 and P-tau levels. Also, amygdalin might be one of the compounds responsible for the transiently increased intracerebral Aβ42 levels.

DOI： 10.1038/s41598-023-44098-3

Web of Science

Scopus

PubMed

記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Life Sciences  

Aims: Idiopathic pulmonary fibrosis (IPF) is a severe pulmonary interstitial pneumonia. Our study focuses on the role of PLA2 enzyme in the IPF to explore a more effective diagnosis and treatment mechanism of IPF. Main methods: Transcriptome data of IPF from GEO database and bleomycin-induced pulmonary fibrosis mice were analyzed to identify PLA2 enzyme and their metabolite, lysophosphatidylcholines 18:0, in IPF. Based on PLA2G2A and PLA2G2D / PLA2G2A-associated cell death genes (PCDs), the consensus clustering analysis was used to identify the subtypes of IPF and the correlation between PLA2G2A and prognosis was analyzed. The machine learning (ML) models and artificial neural network (ANN) model was used to validate the diagnostic accuracy of PLA2s and PCDs in diagnosing IPF. The gene and protein expression of NLRP3, GSDMD, and CASP-1 was estimated in recombinant PLA2G2A protein induced MLE-12 cells. Key findings: The expression of PLA2G2D, PLA2G2A, and LPC18 significantly changed in IPF. Furtherly, PLA2G2A has a significant correlation with poor patient prognosis, which could predict the 2 or 3-years mortality rates of IPF. Two subtypes of IPF patients, identified based on PCDs, showed significant different immunoinfiltration. Recombinant PLA2G2A protein could induce the pyrotosis in the MLE-12 cell. The generalized linear model and ANN model of PLA2s or PCDs accurate diagnosis IPF. Significance: PLA2G2A is the most robustly associated gene with IPF among the PLA2s, which demonstrates a potential in diagnosing and prognostic value in IPF, and provides a foundation for further understanding and breakthroughs in IPF diagnosis and treatment.

DOI： 10.1016/j.lfs.2023.122071

Web of Science

Scopus

PubMed

記述言語：英語   出版者・発行元：Planta Medica  

A large variety of natural plants are widely produced and utilised because of their remarkable pharmacological effects. In this study, two phenolic glycosides were isolated for the first time from Vanilla pompona Schiede (Orchidaceae) from Kyushu, Japan: bis [4-(β -D - O-glucopyranosyloxy)-benzyl] (S)-2-isopropylmalate (1) and bis 4-[ β -D- O -glucopyranosyloxy)-benzyl]-(2 R,3 S)-2-isopropyl tartrate (2). We have discovered that the crude extract of V. pompona leaves and stems and its two phenolic glycosides (compounds 1  -  2) are highly effective in reversing skin senescence. V. pompona and compounds 1  -  2 were found to promote the synthesis of collagen, hyaluronic acid, and elastin in skin fibroblasts in a normal skin cell model; in a replicative senescence model, V. pompona and compounds 1  -  2 significantly reduced the ageing phenotype in skin fibroblasts. These compounds also demonstrated a significant protective effect in a UV-induced photo-senescence model; the possible mechanisms of this effect were investigated in this study. To the best of our knowledge, this study is the first to develop V. pompona leaves and stems as new sources of bioactive compounds and to examine their therapeutic potential for skin senescence. The development potential of V. pompona leaves and stems for use in the cosmetics, cosmeceutical, and pharmaceutical industries remains to be investigated.

DOI： 10.1055/a-2117-9233

Web of Science

Scopus

PubMed

記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：ACS Omega  

Sixteen chemically varied metabolites were isolated from the bulbs of Hippeastrum vittatum (L’Her.) Herb., including eight flavonoids [3′-methyl isoliquiritigenin (2), 7-hydroxyflavan (8), 7-hydroxyflavanone (9), 7-hydroxyflavan-3-ol (10), 7-methoxy-3′,4′-methylenedioxyflavan-3-ol (11), 7-hydroxy-3′,4′-methylenedioxy flavan (12), 2′,4′-dihydroxy-3′-methyl-3,4-methylenedioxychalcone (13), and isoliquiritigenin (14)], four acetophenones [2,6-dimethoxy-4-hydroxyacetophenone (3), 2,4-dihydroxyacetophenone (4), 2,4-dihydroxy-6-methoxy-3-methylacetophenone (6), and 2,4,6-trimethoxyacetophenone (7)], two alkaloids [lycorine (1) and narciprimine (15)], one phenol derivative [p-nitrophenol (5)], and one steroid [β-sitosterol 3-O-β-glucopyranoside (16)]. Their structures were elucidated by combining one- and two-dimensional NMR and ESI-MS techniques and by comparison with the reported literature data and some authentic samples. Except for lycorine (1), the isolated metabolites were obtained herein for the first time from Hippeastrum plants, among which compound 13 was identified as a new chalcone derivative. Additionally, the total phenolic and flavonoid contents of the total ethanol extract and different fractions of the bulbs were determined by the Folin-Ciocalteu and aluminum chloride colorimetric methods, respectively, whereas their antioxidant potential was compared using the phosphomolybdenum and 2,2′-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging assays. Finally, the binding affinities of compounds 1-16 to some key target proteins of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), namely, main protease (Mpro), papain-like protease (PLpro), and RNA-dependent RNA polymerase (RdRp), were screened and compared using molecular docking analysis. The possible chemotaxonomic significance of the identified metabolites was also discussed.

DOI： 10.1021/acsomega.2c07886

Web of Science

Scopus

PubMed

記述言語：日本語   出版者・発行元：(株)インフォノーツパブリッシング  

記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：RSC Advances  

Oil spills that contaminate the environment can harm the surrounding ecosystem. The oil contains petroleum hydrocarbon which is toxic to the environment hence it needs to be removed. The use of bacteria as remediation media was modified by immobilizing into a matrix hence the bacteria can survive in harsh conditions. In this research, the ability of biosurfactant-producing bacteria (Pseudomonas aeruginosa, Bacillus subtilis, and Ralstonia pickettii) immobilized in the PVA/SA/bentonite matrix was tested in remediation on oil-contaminated soil. The immobilized beads filled with bacteria were added to the original soil sample, as well as washed soil. The beads were characterized by using FTIR and SEM. Based on FTIR analysis, the PVA/SA/bentonite@bacteria beads had similar functional groups compared to each other. SEM analysis showed that the beads had non-smooth structure, while the bacteria were spread outside and agglomerated. Furthermore, GC-MS analysis results showed that immobilized B. subtilis and R. pickettii completely degraded tetratriacontane and heneicosane, respectively. Meanwhile, after soil washing pre-treatment, immobilized bacteria could completely degrade octadecane (P. aeruginosa and R. pickettii) and tetratriacontane (P. aeruginosa and B. subtilis). Based on those results, immobilized bacteria could degrade oil compounds. The degradation result was influenced by the enzymes produced, the ability of the bacteria, the suitability of the test media, and the matrix used. Therefore, this study can be a reference for further soil remediation using eco-friendly methods.

DOI： 10.1039/d3ra02249h

Web of Science

Scopus

PubMed

記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Molecules  

Hericium erinaceus, a mushroom species commonly known as Yamabushitake in Japan, is known to have a stimulatory effect on neurotrophic factors, such as brain-derived neurotrophic factor (BDNF) and nerve growth factor (NGF). Hericenone C, a meroterpenoid with palmitic acid as the fatty acid side chain, is reported to be one such stimulant. However, according to the structure of the compound, the fatty acid side chain seems highly susceptible to lipase decomposition, under in vivo metabolic conditions. To study this phenomenon, hericenone C from the ethanol extract of the fruiting body was subjected to lipase enzyme treatment and observed for changes in the chemical structure. The compound formed after the lipase enzyme digestion was isolated and identified using LC-QTOF-MS combined with 1H-NMR analysis. It was found to be a derivative of hericenone C without its fatty acid side chain and was named deacylhericenone. Interestingly, a comparative investigation of the neuroprotective properties of hericenone C and deacylhericenone showed that the BDNF mRNA expression in human astrocytoma cells (1321N1) and the protection against H2O2-induced oxidative stress was considerably higher in the case of deacylhericenone. These findings suggest that the stronger bioactive form of the hericenone C compound is in fact deacylhericenone.

DOI： 10.3390/molecules28114549

Web of Science

Scopus

PubMed

researchmap

記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Cosmetics  

Nutritional foods are concentrated sources of molecules with a nutritional or physiological effect which contain nutrients. There is a category, “nutricosmetics”, defined as ingestible natural health products that enhance the function and appearance of human skin, nails, and hair. A new variety of Flammulina velutipes (F. velutipes), Oki-Shirayuki 919, was explored to ascertain whether its components have functions of nutricosmetics. We focused on physiological effects for enhancing the human skin condition, such as moisturizing or barrier functions in F. velutipes. A randomized, double-blind, placebo-controlled clinical study was performed between January and March 2022. Among healthy men and women (n = 30) aged 20 to 59 years, the test group (n = 15) took a test product which included F. velutipes dry powder, and the placebo group (n = 15) took a placebo (a similar product in which the F. velutipes dry powder was replaced with plum fruit paste). Since the amount of increase in skin hydration over four weeks in the test group was significantly larger than that in the placebo group, a significant difference between the two groups was observed (p = 0.033). F. velutipe was suggested to have some physiological functions such as improving skin moisture.

DOI： 10.3390/cosmetics10020057

Web of Science

Scopus

記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Planta Medica  

Although considered an abundant source of agricultural by-products, avocado (Persea americana Mill.) seed, with several biological activities and bioactive components, might become a promising resource for phytopharmaceutical development. In this study, through bioassay-guided isolation of the main α -glucosidase inhibitors in avocado seed, we discovered the major α -glucosidase inhibitor to be avocado seed oligomeric proanthocyanidin complex (ASOPC). Thiolysis and UPLC-DAD-HRESIMS showed the presence of A- and B-type procyanidins, and B-type propelargonidin with (epi)afzelechin as extension unit. Mean degree of polymerization (mDP) of ASOPC was calculated as 7.3 ± 1. Furthermore, ASOPC appeared to be a strong, reversible, competitive inhibitor of α -glucosidase, with IC 50value of 0.1 μg/mL, which was significantly lower than Acarbose (IC 50= 75.6 μg/mL), indicated that ASOPC is a potential natural α -glucosidase inhibitor. These findings would contribute to the direction of utilizing avocado seed bioactive components with the possibility to be used as natural anti-diabetic agents.

DOI： 10.1055/a-1878-3916

Web of Science

Scopus

PubMed

記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Natural Product Communications  

Since the recent coronavirus disease 2019 pandemic and the lifestyle changes it necessitated, the demand for mental health treatment has skyrocketed, with long wait lists for both psychological and psychiatric care. Over-the-counter supplements and home remedies are increasingly sought. In this study, we screened natural materials and blended supplements from Asia that may improve the mood and mental health of humans by testing cell viability and expression of the proopiomelanocortin gene as a marker of β-endorphin production in rat hypothalamus neuron cells. Among 23 tested samples, 3 samples produced significantly higher cell viability in R-HTH-507 cells than the control treatment. In a real-time-polymerase chain reaction (RT-PCR) experiment, 7 samples showed significant β-endorphin synthesis activity. This is the first report that the Asian natural materials Areca catechu, Moringa oleifera, Lignosis rhinocerus, and Aegle marmelos promote β-endorphin synthesis; further investigation will identify the active ingredients in the blended samples. These results suggested that these Asian natural materials have great potential to expand the range of treatments for mental health.

DOI： 10.1177/1934578X231152964

Web of Science

Scopus

記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Science of the Total Environment  

Accumulating evidences show that the hazardous substance atmospheric nanoplastics increase the respiratory risk of individuals, but the inside toxicity mechanisms to lung tissue remain unclear. This study aims at investigating the potential mechanisms of inhaled cationic polystyrene nanoplastics (amine-polystyrene nanoplastics, APS-NPs)-induced pulmonary toxicity on mice. In vivo, the mice intratracheal administrated with APS-NPs suspension (5 mg/kg) were found inflammatory infiltrates in lung tissues through histopathology analysis. Furthermore, transcriptome analysis demonstrated that 1821 differentially expressed mRNA between APS group and control group were dominantly associated with 288 known KEGG pathways, indicating that APS-NPs might cause early inflammatory responses in lung tissue by activating the NLRP3/capase-1/IL-1β signaling pathway. Moreover, in vitro results also showed that NLRP3 inflammasome could be activated to induce pyroptosis in MLE-12 cells after exposure to APS-NPs. And, MH-S cells after exposure to APS-NPs exhibited increased Irg1 proteins, leading to the increasing generation of ROS and inflammatory factors (e.g., tnf-α, il-6, il-1β). In conclusion, these results revealed that Irg1/NF-κB/NLRP3/Caspase-1 signaling pathway was activated significantly after exposing to APS-NPs, leading to pulmonary toxicity on mice. Intriguingly, prior administration of the clinical antioxidant N-acetylcysteine (NAC) could serve as a possible candidate for the prevention and treatment of pulmonary toxicity induced by APS-NPs. The study contributes to a better understanding of the potential risks of environmental nanoplastics to humans and its improvement measure.

DOI： 10.1016/j.scitotenv.2022.158851

Web of Science

Scopus

PubMed

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： DOI: 10.1002/jssc.202200717

記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Microchemical Journal  

A green vortex-assisted dispersive liquid–liquid microextraction (VA-DLLME) method based on a hydrophobic natural deep eutectic solvent (NaDES) was developed and applied for the preconcentration of anti-prostate cancer triple therapy (flutamide, resveratrol, and ethynylestradiol) from river water and spiked human plasma for the first time. A hydrophobic NaDES based on α-terpineol and octanoic acid was prepared in a molar ratio of 1:1 and applied as an extracting solvent. High-performance liquid chromatography with a photodiode array detector (HPLC-PDA), coupled with a core–shell-based column was used for the final analysis. The significant factors affecting the extraction efficiency were optimized by means of a systematic methodology of one-variable-at-a-time (OVAT) followed by experimental design. The optimal extraction conditions were as follows: 400 μL of DES, vortex time of 50 sec, pH 2.5, and 3 % of NaCl. Under optimal conditions, the method was validated through matrix-matched calibration, limits of detection and quantitation, accuracy, and precision studies. The method exhibited excellent linearity (r2 > 0.999) over a range of 1–30 μg/L for ethynylestradiol and 0.5–30 μg/L for both flutamide and resveratrol with % relative standard deviation (%RSD) values ≤ 4.87 %. The method was successfully applied for the analysis of the concerned drugs in spiked human plasma and river water samples. Finally, the procedure's greenness was assessed using three various greenness metrics which confirmed its greenness and safety.

DOI： 10.1016/j.microc.2022.108124

Web of Science

Scopus

記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Fitoterapia  

We collected stingless bee propolis Tetragonula biroi in order to find materials for medicine and cosmetics applications from tropical rainforest resources. Even though this bee has some biological functions including a cancer cell line, hair growth promotion, asthma remedy, α-glucosidase enzyme inhibition, and antiviral action, the investigation on anti-acne has not been reported yet. This study was to focus on propolis Tetragonula biroi extracts and leads us to isolate active compounds for antioxidant, anti-inflammatory, and anti-acne. We used methanol to obtain the extract from this propolis and assayed it with antioxidants, anti-inflammation, and anti-acne. The extract showed strong activity in antioxidants by DPPH radical scavenging activity (82.31% in 6.25 μg/ml). Via a column chromatography and Reveleris PREP purification system, we isolated 3’–O–methyldiplacone, nymphaeol A, and 5,7,3′,4′–tetrahydroxy–6–geranyl flavonol. These compounds showed potential biological activity with IC50 for antioxidant 6.33, 15.49, 17.32 μM; and antiinflammatory 121.54, 121.20, 117.31 μM. The isolated compounds showed anti-acne properties with properties 0.00, 14.11, and 13.78 mm for the inhibition zone (at a concentration of 1 μg/well), respectively. The results indicated that the propolis extract of Tetragonula biroi has the potential to be developed as a cosmetic agent; however, further work needs to be done to clarify its application.

DOI： 10.1016/j.fitote.2022.105375

Web of Science

Scopus

PubMed

記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Molecules  

This study targets the evaluation of melanin synthesis inhibition activity of the bamboo shoot skin as agro-waste. The total methanolic extract of bamboo peel extract was evaluated for its skin protective effects via measuring its melanin inhibitory activity and its suppression activity on the expression of tyrosinase mRNA levels. Results showed that bamboo peel extract has a good ability for the inhibition of melanin synthesis so further studies were performed for the isolation of its constituents. Twelve compounds have been isolated from the shoot skin of Phyllostachys pubescens. Their structures were elucidated based on extensive spectroscopic methods. The melanin inhibition potential of the isolates was tested with their collagen-production-promoting activity for the determination of active principles. Results showed that Betulinic acid, tachioside, and 1,2-dilinolenin significantly suppressed melanin production per cell compared to control. Triacontanol, tricin, and (+)-lyoniresinol 9′-O-glucoside also tended to decrease melanin production per cell. These findings indicated that the skin of bamboo shoots, a significant agricultural waste, is a useful natural source for further research on its potential for aging problems such hyperpigmentation and cognitive function impairment.

DOI： 10.3390/molecules28010023

Web of Science

Scopus

PubMed

記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Natural Product Research  

Four new natural compounds named hericenone O (1), hericenone P (2), hericenone Q (3), and hericenone R (4), two of them were reported synthetically (3–4), together with eleven known compounds were isolated from the fruiting bodies of Hericium erinaceus. The chemical structures of the isolated compounds were elucidated by using NMR analysis and mass spectrometry, as well as comparisons with the reported data in the literature. The bioactivity evaluation revealed that hericenone Q showed significant cytotoxic activity against Hep-G2 with IC50 values of 23.89 μM, and against HCT-116 with IC50 values of 65.64 μM.

DOI： 10.1080/14786419.2023.2169687

Web of Science

Scopus

PubMed

記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Natural Product Research  

Juncus decipiens is a member of the Juncaceae family and has culinary, medicinal, and decorative properties. It is also used in traditional Chinese Medicines for many years that promotes diuresis for strangury and clears out heart fire. This species has recently gained medicinal attention as a source of phenanthrenes, phenolic compounds, glycerides, flavonoids, and cycloartane triterpenes. This plant was also shown to be active, and researchers explored its antioxidant, anti-inflammatory, antialgal, antibacterial, and psychological behaviour-boosting properties. Preliminary research suggests that this species might be used for skin protection and brain disorders if proper clinical trials are conducted. The ethnomedicinal, phytochemistry, biological potencies, dangers, and scopes of Juncus decipiens have been examined in this respect.

DOI： 10.1080/14786419.2023.2223749

Web of Science

Scopus

PubMed

記述言語：英語   出版者・発行元：Journal of Separation Science  

New ternary deep eutectic solvents were prepared and applied as efficient green dispersing solvents in miniaturized matrix solid-phase dispersion to extract chlorophenols from river sediments for the first time. High-performance liquid chromatography coupled with a photodiode array detector was used to analyze the target analytes. The significant factors affecting the extraction were optimized as follows: dispersant (100 mg), sample (100 mg), ternary eutectic solvents (150 μl), grinding for 1 min, 450 μl of acetonitrile as the elution solvent, and vortex mixing for 20 s. Under the optimal conditions, the method exhibited excellent linearity (correlation coefficient > 0.9980), low limits of detection between 1.039–2.478 μg/g, and extraction recoveries between 93.9% and 99.2%. Furthermore, the method demonstrated excellent precision in the intra- and inter-day analysis with a relative standard deviation below 6%. When compared to conventional extraction techniques, the miniaturized matrix solid-phase dispersion considerably reduced samples and solvent usag, offering important environmental benefits. The green profile of the method was assessed using the complementary green analytical procedure index tool confirming its eco-friendship. The technique was finally employed to evaluate sediment samples from three distinct locations along the Zuibaiji River, indicating its applicability for monitoring environmental samples.

DOI： 10.1002/jssc.202200717

Web of Science

Scopus

PubMed

記述言語：英語   掲載種別：研究論文（学術雑誌）  

記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Natural Product Communications  

Bassia indica (Wight) A.J. Scott, family Amaranthaceae, is a halophyte herb growing in extreme environments and hence deemed as a potential economic source of bioactive chemicals with functional properties. In our study, 25 compounds were obtained from B. indica. We aimed to assess the inhibitory effect of the methanol extract of B. indica and its isolated compounds on COX-2 and cytotoxicity activity against MCF-7, OVK-18, HepG2, and HCT116 tumor cells. Among the isolates, the triterpene oleanane saponin (23) displayed promising anti-inflammatory activity with an IC50 = 3.05 ± 0.15 μg/mL. Additionally, N-trans-feruloyl tyramine (11) exhibited significant cytotoxicity to OVK-18 with IC50 = 1.74 ± 1.56 μg/mL, whereas 6,7-dihydroxy coumarin (7) exhibited potent inhibition against the MCF-7 cell line with IC50 = 1.47 ± 0.22 μg/mL. Interestingly, compounds 1 and 25 exhibited remarkable cytotoxicity against HepG2 and HCT116 cells with IC50 < 0.1 μg/mL, while compounds 2, 4, 5, 6, and 9 exerted potent cytotoxicity against HepG2. Finally, B. indica is a potential source of candidate compounds for the development of anti-inflammatory and antitumor therapies.

DOI： 10.1177/1934578X221137412

Web of Science

Scopus

記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：International Journal of Molecular Sciences  

Hyperpigmentation is a skin condition where patches of skin become darker in color due to excess melanin production upon UV exposure leading to melasma, which are lentigines or post inflammatory hyperpigmentation that psychologically affecting a great number of people. The present study investigates the anti-melanogenic effect of Butyroside D and the underling mechanism. After the confirmation of the non-cytotoxic effect of Butyroside D on B16F10 cells, we proceeded with analyzing the impact of the treatment at low and high concentration (i.e., 0.2 μM and 2 μM) using gene profiling analysis and examined the differentiation in gene expression. Our results identify cyclic adenosine monophosphate (cAMP), Wnt/β-catenin and Mitogen-Activated Protein Kinase (MAPK) signaling pathways to be downregulated upon treatment with Butyroside D. These pathways were targeted to further validate the effect of Butyroside D on membrane receptors melanocortin 1 receptor (MC1R) and receptor tyrosine kinase (c-Kit), related microphthalmia-associated transcription factor (MITF) and consequently tyrosinase (TYR), and tyrosine-related protein-1 (TYRP-1) that were all shown to be downregulated and, therefore, leading to the repression of melanin biosynthesis. Finally, the anti-melanogenic effect of Butyroside D was confirmed on human epidermal melanocytes (HEM) cells by inhibiting the activation of cAMP pathway generally mediated through α-melanocyte-stimulating hormone (α-MSH) and MC1R. Overall, this study suggests the potential applicability of this purified compound for the prevention of hyperpigmentation conditions.

DOI： 10.3390/ijms232416021

Web of Science

Scopus

PubMed

記述言語：英語   掲載種別：研究論文（学術雑誌）  

記述言語：英語   掲載種別：研究論文（学術雑誌）  

記述言語：日本語   出版者・発行元：(株)医事出版社  

肌トラブルの予防や軽減対策となる健康食品のヒト皮膚に及ぼす影響を明らかにするため、ヒハツモドキ(Piper retrofractum)果実抽出物の臨床試験を実施し、角層水分量、経皮水分蒸散量、メラニン・紅斑量の変化を調査した。令和2年10月～令和2年12月にかけて、日常的な肌の乾燥を感じている健康な成人女性39名を被験者とし、試験品群20名(平均48.9±6.9歳)とプラセボ群19名(平均48.4±6.1歳)に割り付けた。試験品はヒハツモドキ抽出物含有サプリメント(300mg/1粒)およびプラセボ(300mg/1粒)の2種、それぞれ1日あたり4粒(1200mg)とした。水分量、経皮水分蒸散量およびメラニンは時間(事前0週、中間4週、事後8週)と群の二要因間に有意な交互作用は認められず、試験品群、プラセボ群の測定値は同様の変化パターンを示した。紅斑について2群間に異なる変化パターンがみられた。肌状態の主観的評価の前後比較では、試験品群では13項目中7項目、プラセボ群では3項目が改善を示した。本試験の期間中、副作用または有害事象は観察されなかった。

記述言語：英語   掲載種別：研究論文（学術雑誌）  

記述言語：英語   掲載種別：研究論文（学術雑誌）  

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： doi:10.1016/j.phytochem.2022.113320

記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Molecules  

Chemical investigation of the total extract of the Egyptian soft coral Heteroxenia fuscescens, led to the isolation of eight compounds, including two new metabolites, sesquiterpene fusceterpene A (1) and a sterol fuscesterol A (4), along with six known compounds. The structures of 1–8 were elucidated via intensive studies of their 1D, 2D-NMR, and HR-MS analyses, as well as a comparison of their spectral data with those mentioned in the literature. Subsequent comprehensive in-silico-based investigations against almost all viral proteins, including those of the new variants, e.g., Omicron, revealed the most probable target for these isolated compounds, which was found to be Mpro. Additionally, the dynamic modes of interaction of the putatively active compounds were highlighted, depending on 50-ns-long MDS. In conclusion, the structural information provided in the current investigation highlights the antiviral potential of H. fuscescens metabolites with 3β,5α,6β-trihydroxy steroids with different nuclei against SARS-CoV-2, including newly widespread variants.

DOI： 10.3390/molecules27217369

Web of Science

Scopus

PubMed

記述言語：日本語   出版者・発行元：ライフサイエンス出版(株)  

薬用化粧品「白陽クリーム」を、30歳以上59歳以下の健常な女性を対象として1日2回朝・晩に顔の片側の目尻にのみに4週間使用し、目尻の写真から専門の検査員がシワグレードを判定するとともに被験者へのアンケートによって肌の状態の変化を検査した。解析対象数はn=14であり、対象者の年齢は38～54歳、平均47.8±5.0歳であった。試験品使用4週間前後における目尻のシワの変化について解析した。その結果、試験品使用側の目尻においては統計的有意なシワグレードの減少(シワの改善)が観測された。不使用側のシワグレードはわずかな増加を示した。使用側/不使用側シワ変化の群間比較からも統計的有意な差異が示唆され、試験品使用側の目尻におけるシワの改善効果が明らかとなった。被験者アンケートからはシワの改善に関しては統計的有意には至らなかったものの、試験品不使用側の得点が減少(状態の悪化)を示すのに対して、使用側の得点は上昇を示していた。

記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Sustainable Chemistry and Pharmacy  

Herein, a green dispersive liquid-liquid microextraction (DLLME) based on hydrophilic and hydrophobic deep eutectic solvents (DESs) was developed for the enrichment of bicalutamide (BIC) from water and spiked human plasma. Six hydrophobic and three hydrophilic DESs were synthesized and evaluated. The hydrophilic DES, composed of tetrabutylammonium bromide and acetic acid in a molar ratio of 1:2, was used as a dispersive solvent. Moreover, the hydrophobic DES, composed of borneol and capric acid in a molar ratio of 1:2, was applied as an extracting solvent. Significant factors affecting the extraction efficiency were investigated through the one-factor-at-a-time (OFAT) strategy. The optimized method was validated in terms of linearity, limits of detection and quantitation, accuracy, precision, and matrix effect. Good linearity with an excellent correlation coefficient (r2 > 0.9999 and 0.9987) was achieved as well as low limits of detection of 0.022 and 0.048 μg/mL and preconcentration up to 53.2 and 92.1 folds for water and plasma, respectively. The proposed DLLME method was coupled with spectrophotometric detection and applied for the analysis of BIC in river water and spiked human plasma with satisfactory recoveries (92.54–99.39%). Finally, the greenness of the developed procedure was evaluated using Complex Green Analytical Procedure Index (ComplexGAPI) and Analytical GREEnness (AGREEprep) tools, exhibiting excellent greenness in comparison to the previously reported methods.

DOI： 10.1016/j.scp.2022.100825

Web of Science

Scopus

記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Natural Product Research  

Two new compounds, 11S-methoxy-11,12-dihydro phytuberin (2) and 9S-methoxy-benzocyclononan-7-one (6), together with twenty-six known ones were isolated from Lycium schweinfurthii (Solanaceae). Their planar structure was established by extensive spectroscopic analyses. The absolute configuration of compound 6 was determined by time dependent density functional theory calculations (TDDFT). The cytotoxic potential of the isolates was assessed in cultured skin cancer (G-361) and colon cancer (HCT-116 and CaCo-2) cell lines. Certain flavonoids showed the highest cytotoxic activity, with IC50 values ranging from 7.1 to 63.3µM; meanwhile 5-flurouracil showed IC50 values ranging from 62.4 to >100 µM. All compounds showed minimal toxicity towards normal cells from skin (NHDF-4) and colon (CCD-841), indicating their potential selectivity and safety as cytotoxic candidates.

DOI： 10.1080/14786419.2021.1922902

Web of Science

Scopus

PubMed

記述言語：英語   出版者・発行元：Phytochemistry  

The phytochemical study of Agathophora alopecuroides (Chenopodiaceae) led to the isolation of previously undescribed glucosylceramide, flavonol triglycoside, and triterpene oleanane saponin, together with eight known compounds. Their structures were elucidated using NMR analysis and HR-MS as (2′R, 12E) N-[(2S, 3S, 4R)-1-(β-D-glucopyranosyloxy)-3,4-dihydroxy-octadec-2-yl]-2-hydroxytetracos-12-enamide, namely Agathophamide A; isorhamnetin-3-O-[β-D-xylopyranosyl-(1→3)-α-L-rhamnopyranosyl-(1→6)]-β-D-galactopyranoside, namely Agathophoroside A; and 3-O-[4'-(β-D-xylopyranosyl)-β-D-glucuronopyranosyl]-28-O-β-D-glucopyranosyl-olean-12-en-3β-ol-28-oic acid, namely Solysaponin A. We evaluated the effect of extract and isolates on ceramide levels via the up-regulated expression of the enzyme for ceramide synthesis in HaCaT keratinocytes. Interestingly, the study results revealed that the methanol extract of A. alopecuroides, together with some isolated compounds significantly up-regulated the mRNA expression of ceramide synthase-3 by 1.2- to 4.3-fold compared with the control in HaCaT cells. These findings indicate that the halophyte A. alopecuroides is a promising source of candidate compounds that can contribute to ceramide synthesis via the up-regulated expression levels of ceramide synthase-3 in the ceramide synthesis pathway.

DOI： 10.1016/j.phytochem.2022.113320

Web of Science

Scopus

PubMed

出版者・発行元：Journal of Forest Research  

Although Chamaecyparis obtusa reduces soil pH and cation levels, limited information is available regarding their alterations after clear-cutting. We examined the soil pH and base cations on the upslopes and downslopes of individual trees and stumps of C. obtusa at two distances (0.5 m and 1.0 m) and three depths (0–5, 5–10, and 10–30 cm) in intact- and cut-tree plots. The soil pH was higher in the cut-tree plot than in the intact-tree plot, indicating the recovery of soil pH after clear-cutting. The recovery was more evident in the surface soils (0–5 cm) of downslope areas (0.5 m) than in other soil depths, which implies that the recovery of soil pH was caused by the lack of H+ supply from stumps after clear-cutting. The results of H+ (in H2O: water-soluble and KCl: exchangeable) suggested a lack of soluble H+ in the cut-tree plot. Soil Ca2+ and Mg2+ levels in the surface soil (0–5 cm) were 1.86-fold and 1.93-fold higher in the cut-tree plot than in the intact-tree plot, respectively, implying that the recovery of divalent cations after clear-cutting was caused by a reduction in cation loss through cation exchange with H+ derived from the stemflow. Collectively, soil pH and divalent cations were recovered after clear-cutting because the stemflow had ceased.

DOI： 10.1080/13416979.2022.2048987

Web of Science

Scopus

記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Molecules  

Hibiscus sabdariffa L. (HS) has a long history of edible and medicinal uses. In this study, the biological activities of the extracts, chromatographic fractions, and hibiscus acid obtained from HS were evaluated for their potential bioactivities. Their ability to promote extracellular matrix synthesis in skin fibroblasts was evaluated by enzyme-linked immunosorbent assays. Their anti-inflammatory activity was evaluated in a nitric oxide (NO)–Griess inflammatory experiment. Furthermore, hibiscus acid was found to have a strong anti-oxidative stress effect through the establishment of an oxidative stress model induced by hydrogen peroxide. Several assays indicated that hibiscus acid treatment can effectively reduce extracellular adenosine triphosphate (ATP) secretion and carbonyl protein production, as well as maintain a high level of reduced/oxidized glutathione (GSH/GSSG) in skin cells, thus providing a possible mechanism by which hibiscus acid can counter antioxidative stress. The present study is the first to explore the reversing skin aging potential and the contributory component of HS.

DOI： 10.3390/molecules27186076

Web of Science

Scopus

PubMed

記述言語：日本語   出版者・発行元：ライフサイエンス出版(株)  

サジー果汁飲料の摂取による心身の疲労感に対する改善効果を検証するために、健常中年女性を対象に、4週間のプラセボ対照二重盲検ランダム化並行群間比較試験を実施した。対象者39名を無作為に割り付け、9名を除外し最終的に介入群14名(平均45.1±0.3歳)、プラセボ群16名(平均44.8±0.3歳)と設定した。対象者は、与えられた試験食品またはプラセボを1日30mL、4週間摂取した。心身の疲労に関する評価(visual analogue scale法)は心身疲労に関する自己評価アンケートにより実施した。心身の疲労時に現れる症状12項目(全体の疲労感、精神的疲労感、身体的疲労感、自覚的ストレス、緊張度、眠気、退屈度、意欲、空腹度、のどの渇き、イライラ感、爽快感)について、VAS法を使用した。心身の疲労に関する評価VASの結果、介入前後の差では「空腹感」のみに、介入群とプラセボ群で有意な差がみられた。介入前後の変化では、介入群のみに有意な減少がみられ、プラセボ群は有意でないものの平均値の上昇がみられた。安全性評価において、試験期間中に有害事象は認められなかった。

記述言語：日本語   掲載種別：研究論文（大学，研究機関等紀要）   出版者・発行元：九州大学大学院農学研究院  

DOI： 10.15017/4797835

CiNii Research

researchmap

記述言語：英語   掲載種別：研究論文（学術雑誌）  

記述言語：英語   出版者・発行元：Molecules  

Averrhoa carambola L. is reported for its anti-obese and anti-diabetic activities. The present study aimed to investigate its aqueous methanol leaf extract (CLL) in vivo anti-obese activity along with the isolation and identification of bioactive compounds and their in vitro α-glucosidase inhibition assessment. CLL improved all obesity complications and exhibited significant activity in an obese rat model. Fourteen compounds, including four flavone glycosides (1–4) and ten dihydrochalcone glycosides (5–12), were isolated and identified using spectroscopic techniques. New compounds identified in planta included (1) apigenin 6-C-(2-deoxy-β-D-galactopyranoside)-7-O-β-D-quinovopyranoside, (8) phloretin 3′-C-(2-O-(E)-cinnamoyl-3-O-β-D-fucopyranosyl-4-O-acetyl)-β-D-fucopyranosyl-6′-O-β-D fucopyranosyl-(1/2)-α-L arabinofuranoside, (11a) phloretin3′-C-(2-O-(E)-p-coumaroyl-3-O-β-D-fucosyl-4-O-acetyl)-β-D-fucosyl-6′-O-(2-O-β-D-fucosyl)-α-L-arabinofuranoside, (11b) phloretin3′-C-(2-O-(Z)-p-coumaroyl-3-O-β-D-fucosyl-4-O-acetyl)-β-D-fucosyl-6′-O-(2-O-β-D-fucosyl)-α-L-arabinofuranoside. Carambolaside M (5), carambolaside Ia (6), carambolaside J (7), carambolaside I (9), carambolaside P (10a), carambolaside O (10b), and carambolaside Q (12), which are reported for the first time from A. carambola L. leaves, whereas luteolin 6-C-α-L-rhamnopyranosyl-(1-2)-β-D-fucopyranoside (2), apigenin 6-C-β-D-galactopyranoside (3), and apigenin 6-C-α-L-rhamnopyranosyl-(1-2)-β-L-fucopyranoside (4) are isolated for the first time from Family. Oxalidaceae. In vitro α-glucosidase inhibitory activity revealed the potential efficacy of flavone glycosides, viz., 1, 2, 3, and 4 as antidiabetic agents. In contrast, dihydrochalcone glycosides (5–11) showed weak activity, except for compound 12, which showed relatively strong activity.

DOI： 10.3390/molecules27165159

Web of Science

Scopus

PubMed

出版者・発行元：Revista Brasileira de Farmacognosia  

Phytochemical investigation of Ficus benghalensis L., Moraceae, leaves revealed three known compounds, carpachromene, mucusoside, and 2-O-α-l-rhamnopyranosyl-(2R)-hydroxyhexacosanoate-β-d-glucopyranosyl ester. Herein, the cytotoxic activity of the isolated compounds was measured against the HL60 cancer cell line. Our findings revealed that carpachromene exerted potent anti-proliferative activity. Also, this compound induced apoptosis and cell cycle arrest at the G2/M phase. Docking analysis revealed the potential interaction of carpachromene with topoisomerase I. This was confirmed by western blotting where carpachromene inhibited topoisomerase I protein expression. Additionally, carpachromene upregulated the expression of pro-apoptotic genes. In conclusion, F. benghalensis leaves exerted potent anti-proliferative activity against HL60 cancer cells. [Figure not available: see fulltext.]

DOI： 10.1007/s43450-022-00267-5

Web of Science

Scopus

記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Scientific Reports  

We examined the association of serum s-adenosylmethionine (SAM), s-adenosylhomocysteine (SAH) (methionine metabolites), and their ratio on the risk of dementia and death in a community-dwelling population of older Japanese individuals. 1371 residents of Hisayama, Japan, aged 65 years or older and without dementia, were followed for a median of 10.2 years (2007–2017). We divided serum SAM, SAH, and SAM/SAH ratio into quartiles. Cox proportional hazards models were used to estimate the hazard ratios (HRs) and their 95% confidence intervals (CIs) of serum SAM, SAH, and SAM/SAH ratio levels on the risk of a composite outcome of all-cause dementia or death, and each outcome. During the follow-up, 635 participants developed all-cause dementia and/or died, of which 379 participants developed dementia and 394 deaths occurred. The multivariable-adjusted HRs of the composite outcome decreased significantly with increasing serum SAM levels (P for trend = 0.01), while they increased significantly with higher serum SAH levels (P for trend = 0.03). Higher serum SAM/SAH ratio levels were significantly associated with a lower risk of the composite outcome (P for trend = 0.002), as well as with lower risk of each outcome. Our findings suggest that the balance of methionine metabolites may closely associate with the risk of dementia and death.

DOI： 10.1038/s41598-022-16242-y

Web of Science

Scopus

PubMed

researchmap

記述言語：英語   掲載種別：研究論文（学術雑誌）  

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1016/j.sajb.2022.01.028

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1016/j.sajb.2022.01.028

記述言語：英語   出版者・発行元：RSC Advances  

In Alzheimer's disease (AD), the accumulation of amyloid-β plaques, overactivity of MAO-B, and phosphorylated tau protein in the central nervous system result in neuroinflammation and cognitive impairments. Therefore, the multi-targeting of these therapeutic targets has emerged as a promising strategy for the development of AD treatments. The current study reports the isolation and identification of seven amide alkaloids, namely, N-trans-feruloyl-3-methoxytyramine (1), N-trans-feruloyltyramine (2), S-(−)-N-trans-feruloylnormetanephrine (3), S-(−)-N-trans-feruloyloctopamine (4), R-(+)-N-trans-feruloyloctopamine (5), N-trans-caffeoyltyramine (6), and S-(−)-3-(4-hydroxy-3-methoxyphenyl)-N-[2-(4-hydroxyphenyl)-methoxyethyl]acrylamide (7), from B. indica and A. alopecuroides, which are halophytic plants that have been reported to contain diverse phytochemicals. Additionally, the study explores the potential inhibition effects of the isolates on β-secretase, monoamine oxidase enzymes, and phosphorylated tau protein, and their anti-aggregation effects on amyloid-β fibrils. Compounds 1, 2, and 7 showed potent inhibitory activity against BACE1, MAO-B, and phosphorylated tau protein, as well as anti-aggregation activity against Aβ-peptides. Additionally, compound 6 displayed promising inhibition activity against MAO-B enzyme. Further in-depth in silico and modeling analyses (i.e., docking, absolute binding free energy calculations, and molecular dynamics simulations) were carried out to reveal the binding mode of each active compound inside the corresponding enzyme (i.e., MAO-B and BACE1). The results indicate that B. indica, A. alopecuroides, and the isolated amide alkaloids might be useful in the development of lead compounds for the prevention of neurodegenerative diseases, especially AD.

DOI： 10.1039/d2ra02275c

Web of Science

Scopus

PubMed

出版者・発行元：Journal of Alloys and Compounds  

Photocatalytic treatment is a remarkable process that is popular for the degradation of several organic contaminants in wastewater. In this work, a series of graphitic carbon nitride/ZnTi-mixed metal oxide (CN/ZnTi-MMO) composites were prepared as photocatalysts through simple calcination. The CN-MMO samples were characterized and applied for photocatalytic degradation of ciprofloxacin (CIP), which is an antibiotic model pollutant. The optimized CN/ZnTi-MMO composite showed complete degradation of CIP within 20 min, leading to a rate constant that is approximately 6 times that of pristine CN and 7 times that of ZnTi-MMO. The increase in the degradation rate of the optimized CN/ZnTi-MMO composite was attributed to the excellent separation and transportation of photogenerated electron-holes pairs, as proven by the photoluminescence, photocurrent density, and electrochemical impedance spectroscopy results. Moreover, the energy-resolved distribution of the electron trap (ERDT) pattern of the composite sample suggests the formation of an interfacial electron trap state due to interfacial contraction in the composite, resulting in the excited electrons being trapped and avoiding charge recombination. Based on the optical properties, ERDT results, and activity test, a photocatalytic degradation mechanism of CIP over a CN/ZnTi-MMO composite was proposed. Additionally, the degraded solution showed less bio-toxicity than the original CIP solution. Thus, the CN/ZnTi-MMO composite can be employed as a potential visible-light-driven photocatalyst for the detoxification of pharmaceutical wastewater.

DOI： 10.1016/j.jallcom.2022.164294

Web of Science

Scopus

記述言語：英語   掲載種別：研究論文（学術雑誌）  

記述言語：英語   掲載種別：研究論文（学術雑誌）  

記述言語：日本語   出版者・発行元：(株)医事出版社  

目的および方法:イヌリン含有食品の摂取により、心身の疲労感を自覚する健常人の疲労感・ストレスおよび粘膜免疫に与える効果を検証するために、心身の疲労感を自覚する健常人50名[解析対象者48名(男性24名、女性24名)]を対象にランダム化プラセボ対照二重盲検並行群間比較試験を実施した。摂取前後に唾液検査および質問紙検査を実施した。結果:その結果、POMS2における「疲労-無気力」「緊張-不安」「TMD得点」のスコアが有意に低下および「活気-活力」のスコアが有意に上昇した。視覚的評価スケール(VAS)においては、疲労感に関する項目の低下が認められた。風邪症状においては、「鼻づまり」「頭痛」「倦怠感」「寒気」「総合的な臨床症状」の有症日数に有意な低値が認められた。唾液検査においては、唾液分泌量が有意に増加、さらに唾液IgA分泌速度についても、有意な差は認められなかったもののプラセボ群と比較して増加した。結論:これらの結果からイヌリン含有食品の摂取により疲労感やストレスを軽減し活動的な気分を維持する効果が示唆された。さらに免疫機能向上および風邪症状の発症抑制、軽減効果が示唆された。(著者抄録)

記述言語：日本語   出版者・発行元：ライフサイエンス出版(株)  

サジー果汁飲料の4週間摂取前後における免疫機能の変化を、血液検査、唾液検査、風邪症状や睡眠、腸内環境等を評価する質問紙検査の3種類の検査を実施することにより検証した。被験者37名を対象とし、試験品群18人(男性9人、女性9人、平均44.6歳)、プラセボ群19人(男性9人、女性10人、平均43.1歳)に割り付けた。サジー果汁飲料の常飲が血中の白血球成分に働きかけることで、自然免疫、獲得免疫を活性化している可能性が示唆され、唾液中におけるsIgA濃度の保持にサジー果汁飲料摂取が効果的であることが示された。試験品群にのみ睡眠の質の改善が認められ、気分の改善も確認された。このほか、サジー果汁飲料の常飲によって腸内環境が改善した可能性が考えられ、風邪にかかりにくく、かかったとしても回復のしやすい体調が整えられていることが示唆された。

記述言語：英語   出版者・発行元：Journal of Natural Medicines  

DOI： 10.1007/s11418-022-01611-8

Web of Science

Scopus

PubMed

記述言語：英語   掲載種別：研究論文（学術雑誌）  

記述言語：日本語   出版者・発行元：ライフサイエンス出版(株)  

イヌリン5g含有サプリメントを4週間摂取した際の腸内環境や排便症状、肌質に及ぼす影響について検討するため、便秘傾向にある20歳以上の健康な女性を対象として、プラセボ対照二重盲検ランダム化並行群間比較試験を行った。イヌリン群15名、プラセボ群13名における便の性状と肌状態主観的評価のvisual analogue scale、dermatology life quality index(DLQI)、肌質検査、有害事象を評価した。その結果、イヌリン摂取によって排便に関する指標が改善し、DLQIでは「家事への支障」「仕事効率」への影響が減少した。肌のシワ、たるみ、透明感や化粧のり、顔色などが改善され、経表皮水分蒸散量と皮膚弾力性の変化率に有意な群間差を認めた。本試験において有害事象は認めなかった。イヌリンは肌バリア機能や肌の粘弾性を改善し、肌質を改善することが示された。

記述言語：英語   出版者・発行元：ライフサイエンス出版(株)  

Tongkat Ali(TA)補充がストレスマーカーと睡眠の質に与える影響について、4週間の並行二重盲検プラセボ比較対照試験を行った。健常者33名(30～60歳)をベースラインの年齢とBMIに基づいて、TAサプリメントを摂取するTA群とプラセボ錠を摂取するプラセボ群に配分した。気分プロフィール検査(POMS)とOSA睡眠調査表MA版(OSA-MA)を用いて比較した。4週間目にTA群だけPOMSの活気と友好のスコアが有意に改善した。TA群だけに4週目にOSA-MAの睡眠と起床に、有意なスコアの改善が認められた。

記述言語：英語   出版者・発行元：European Journal of Pharmacology  

Pulmonary fibrosis (PF) is a chronic interstitial lung disease with unknown etiology. In the present study, we evaluated the anti-fibrotic effects of heterophyllin B, a natural product from Radix Pseudostellariae having anti-inflammatory and tyrosinase inhibitory activities. In bleomycin (BLM)-induced PF mouse model, heterophyllin B treatments (5 or 20 mg/kg/d) significantly attenuated BLM-induced alveolar cavity collapse, inflammatory cell infiltration, alveolar wall thickening and collagen deposition. When compared to model group, heterophyllin B treatments also increased adenosine 5′-monophosphate (AMP)-activated protein kinase (AMPK) phosphorylation levels by 359% (P < 0.001) and reduced the expression of stimulator of interferon genes (STING) by 73% (P < 0.001). Furthermore, co-administration of heterophyllin B with AMPK inhibitor dorsomorphin (Compound C) significantly blocked the improvement effects of heterophyllin B on BLM-damaged lung tissue, and also increased the protein expression of STING which was inhibited by heterophyllin B in fibrotic lungs (P < 0.001). It is known that alveolar epithelia and lung fibroblasts exert prominent roles in the fibrosis progression. In the present study we found that, in vitro, heterophyllin B significantly inhibited alveolar epithelial mesenchymal transition (EMT) and lung fibroblast transdifferentiation. We also found that the inhibition of heterophyllin B on lung fibroblast transdifferentiation and STING expression was reversed by Compound C. To summarize, heterophyllin B exhibited protective effects on BLM-induced lung fibrosis potentially by inhibiting TGF-Smad2/3 signalings and AMPK-mediated STING signalings.

DOI： 10.1016/j.ejphar.2022.174825

Web of Science

Scopus

PubMed

記述言語：英語   掲載種別：研究論文（学術雑誌）  

記述言語：日本語   出版者・発行元：(株)医事出版社  

目的:本試験では、「膝に痛みまたは違和感のある者」を対象として、化粧品の塗布とマッサージを併用することにより、持続的な関節(肩、腰、膝))の痛みの緩和効果を検証することを目的とした。試験デザイン:ランダム化・プラセボ対照・二重盲検・並行群間比較試験。方法:30名をランダムに2群に割り付け、試験品またはプラセボの塗布とマッサージを28日間併用し、前後に質問紙検査(NRS、FRS、VRS、VAS)を実施した。結果:各質問紙検査において試験品群の関節(肩、腰、膝)の痛みに有意な低下または低下傾向が認められた。結論:試験品とマッサージを28日間併用することで、関節(肩、腰、膝))に対する痛み緩和効果が示唆された。さらにそれらは持続的にも有効であっことが確認された。(著者抄録)

記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Journal of Chromatography A  

四重極飛行時間型質量分析計と組み合わせた液体クロマトグラフィー（LCQTOFMS）に基づく包括的な非ターゲットスクリーニングワークフローを構築した。本研究により、廃液処理の流出水中内に、さまざまなクラス（主に医薬品と農薬）の74の化合物が同定された。同定された化合物の廃液処理中の生成と除去のプロセスを解明した。さらに、確認された化合物中、いくつかの医薬品がすべてのサンプルで最大の全体濃度を示し、次に難燃剤が多く定量された。非ステロイド系抗アンドロゲンであるビカルタミドは、難燃剤であるリン酸トリス（2-ブトキシエチル）が337.2〜1304.6の範囲の濃度を示した廃水流出液で、最高濃度（843.9〜3838 ng / L）で検出された。医薬品であるテルミサルタンは、潜在的な毒性を示す量存在していることが示された。多変量解析を適用して、廃水処理の効率を評価するとともに、メタボロミクスアプローチにより、流入サンプルと流出サンプルを明確に区別することができ、効果的な排水処理システムについての新規評価方法を提案した。

DOI： 10.1016/j.chroma.2022.462905

Web of Science

Scopus

PubMed

記述言語：英語   掲載種別：研究論文（学術雑誌）  

記述言語：英語   掲載種別：研究論文（学術雑誌）  

記述言語：英語   掲載種別：研究論文（学術雑誌）  

出版者・発行元：Natural Product Communications  

Chronic ultraviolet (UV) exposure produces oxidative stress, molecular damage, and aging-related signal transduction, all of which contribute to skin photoaging. In this study, the antioxidant and anti-inflammatory activities of Entada phaseoloides are reported. High-performance liquid chromatography (HPLC) detected 7 phenolic compounds: gallic acid, protocatechuic acid, 4-hydroxybenzoic acid, quercetin, luteolin, kaempferol, and apigenin. We investigated the antioxidant and protective effect of the acetone extract of E. phaseoloides leaves (AEP) on UVB-irradiated human epidermal keratinocytes (HaCaT cells). AEP showed antioxidant activity in the 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2-azinobis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) assays. AEP at a concentration of 40 μg/mL increased cell survival rate of the UVB-damaged cells. Moreover, AEP blocked gene expression of nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) in UVB-induced HaCaT cells and decreased UVB-induced apoptosis in HaCaT cells by regulating the gene expression of caspase-3. These results suggest that AEP has the potential to protect against UVB irradiation and antioxidant.

DOI： 10.1177/1934578X221078627

Web of Science

Scopus

記述言語：英語   出版者・発行元：Health Science Reports  

Introduction: Placenta extract is used as an ingredient in ointments for treating dermatological diseases, skin dryness, and for skin beautification. However, the clinical effects of the equine placenta on humans and the underlying mechanism of action are unclear. This randomized, controlled, double-blind study aimed to clinically evaluate the effect of oral intake of equine placental extract on human skin quality. Methods: Healthy women volunteers between the ages of 30 and 59 years (n = 29) were randomly assigned to receive 220 mg of equine placental extract–placebo orally, once daily for 4 weeks. Skin quality parameters such as skin hydration, skin barrier function (transepidermal water loss [TEWL]), and melanin index were assessed at baseline and after 4 weeks of administration. Results: The melanin index was significantly increased in the placebo group, whereas it remained unchanged in the equine placenta group. The pattern of melanin index change was significantly different due to intake or no intake of equine placenta supplements over 4 weeks. No significant difference was found in skin hydration and TEWL between the two groups at 4 weeks of postadministration. It was shown that the intake of the equine placenta was more effective in protecting the skin condition against the change of ultraviolet (UV) sensitively than the change in temperature and humidity. Conclusions: Effect of equine placental extract intake was evident on the cheek skin of the equine placenta group where participants were protected from UV-induced pigmentation. Equine placental extract is useful for decreasing melanin synthesis and melanin content in the human skin and can be used as an effective food supplement to maintain human skin quality.

DOI： 10.1002/hsr2.522

Web of Science

Scopus

PubMed

記述言語：英語   掲載種別：研究論文（学術雑誌）  

記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：International Journal of Environmental Research and Public Health  

This study compared the participants’ physiological responses and subjective evaluations of air scented with different concentrations of common rush (Juncus effusus L. var. decipiens Buchen.) (30 g and 15 g, with fresh air as a control). We asked 20 participants to complete a series of visual discrimination tasks while inhaling two different air samples. We evaluated (1) brain activity, (2) autonomic nervous activity, and (3) blood pressure and pulse rate, (4) in combination with self-evaluation. In addition, we quantified the concentrations of volatile organic compounds. The participants reported the scent to be sour, pungent, and smelly; this impression was likely caused by hexanal and acetic acid. Although the self-evaluations showed that participants did not enjoy the scent, their alpha amplitudes of electroencephalogram and parasympathetic nervous activity were increased, suggesting that participants were relaxed in this atmosphere. Moreover, a lower concentration resulted in a greater induction of relaxation. While the air was not pleasant-smelling, the volatile organic compounds present had a positive psychophysiological impact.

DOI： 10.3390/ijerph19031856

Web of Science

Scopus

PubMed

researchmap

記述言語：英語   掲載種別：研究論文（学術雑誌）  

記述言語：英語   掲載種別：研究論文（学術雑誌）  

記述言語：英語   出版者・発行元：Molecules  

The extract from Entada phaseoloides was employed as active ingredients of natural origin into cosmetic products, while the components analysis was barely reported. Using LC-DAD-MS/qTOF analysis, eleven compounds (1–11) were proposed or identified from acetone extract of E. phaseoloides leaves (AE). Among them, six phenolic compounds, protocatechuic acid (2), 4-hydroxybenzoic acid (3), luteolin-7-O-β-d-glucoside (5), cirsimaritin (6), dihydrokaempferol (9), and apigenin (10), were isolated by various chromatographic techniques. Protocatechuic acid (2), epicatechin (4), and kaempferol (11) at a concentration 100 μM increased the HaCaT cells viability of the UVB-irradiated cell without any cytotoxicity effect and reduced the expression of COX-2 and iNOS inflammation gene. Moreover, compounds 2 and 4 could have potent effects on cell migration during wound closure. These results suggest that compounds 2, 4, and 11 from AE have anti-photoaging properties and could be employed in pharmaceutical and cosmeceutical products.

DOI： 10.3390/molecules27020440

Web of Science

Scopus

PubMed

記述言語：英語   出版者・発行元：Phytomedicine  

Background: Bryodulcosigenin (BDG) a cucurbitane-type triterpenoid has been isolated from the roots of Bryonia dioca and possesses marked anti-inflammatory effects, although its beneficial effect against intestinal disorders remains unclear. Purpose: To explore the underlying mechanism of BDG on the dysbiosis of chronic ulcerative colitis (UC) and its associated side-effects on lung tissues. Methods: A chronic UC model was established using 2.5% dextran sulfate sodium (DSS) in mice treated for 64 days and diagnostic assessments, western blot analysis and quantitative real time-PCR were employed to determine the protective mechanism of BDG. Results: Oral administration of BDG (10 mg/kg/day) significantly improved colon length, disease activity index, and alleviated colonic histopathological damage in the DSS-induced colitis mice. BDG not only reversed the TNF-α-induced degradation of tight junction proteins (occludin and ZO-1) but also suppressed the elevated apoptosis seen in intestinal epithelial cells (NCM460). In addition, BDG significantly attenuated damage in alveolar epithelial cells (MLE-12) co-cultured with NCM460 cells under inflammatory conditions. Furthermore, BDG in vivo significantly prevented the symptoms of respiratory disorders and repressed alveolar inflammation by regulating DSS-induced chronic colitis in mice. Conclusion: BDG effectively inhibited the apoptosis of intestinal epithelial cells and suppressed the activation of the NLRP3 inflammasome which resulted in the restoration of the intestinal barrier. Therefore, the enhanced integrity of intestinal epithelial cells produced by BDG intervention contributed to its anti-colitis effects, indicating its great potential as an inhibitor of UC and lung injury. Therefore, restoring intestinal integrity may represent a promising strategy in the prevention of pulmonary disease.

DOI： 10.1016/j.phymed.2021.153814

Web of Science

Scopus

PubMed

記述言語：英語   出版者・発行元：Malaysian Journal of Medical Sciences  

The broader objective of this study is to identify natural materials that might inhibit the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. We have focused on stingless bee honey, which has a unique taste that is both sweet and sour and sometimes bitter. We screened 12 samples of honey from 11 species of stingless bees using an angiotensin-converting enzyme 2 (ACE2)-spike protein-binding assay and phytochemical analysis. Ten of the samples showed inhibition above 50% in this assay system. Most of the honey contained tannins, alkaloids, flavonoids, triterpenoids, carotenoids and carbohydrates. Our findings in this in vitro study showed that honey from stingless bees may have a potent effect against SARS-CoV-2 infection by inhibiting the ACE2-spike protein-binding.

DOI： 10.21315/mjms2022.29.5.15

Web of Science

Scopus

PubMed

出版者・発行元：International Journal of Medicinal Mushrooms  

Epidemiologic studies have shown a high prevalence of multiple sclerosis (MS) in Europe and North America, and a low prevalence in East Asia. Mushrooms contain various biological response modifiers (BRMs) and are widely used in traditional Chinese medicine in East Asian countries. To investigate whether mushrooms have potential beneficial effects on MS, we administered mushrooms to cuprizone (bis-cyclohexanone-oxalyldihydrazone, CPZ)-in-duced MS model mice. This model is used to study the processes of demyelination in the CNS. The CPZ-induced de-myelination is involved in the apoptotic death of mature oligodendrocytes, neuroinflammation, and motor dysfunction. Mice were fed a powdered diet containing 5% each mushroom and CPZ diet for 5 weeks, which coincides with peak demyelination. We measured the body weight of the mice, evaluated their motor function using a rotarod, and quanti-fied the myelin levels using Black-Gold II staining. Ganoderma lucidum and Hericium erinaceus treatments showed recovery from weight loss. Pleurotus eryngii, G. lucidum, and Flammulina velutipes treatments significantly improved CPZ-induced motor dysfunction. P. eryngii, G. lucidum, F. velutipes, and H. erinaceus treatments effectively suppressed CPZ-induced demyelination. The four medicinal mushrooms may be promising BRMs for prevention and alleviation of the symptoms of MS.

DOI： 10.1615/IntJMedMushrooms.2022044840

Scopus

出版者・発行元：Journal of Food and Drug Analysis  

Shiikuwasha (Citrus depressa Hayata) essential oil (SEO) extracted from Shiikuwasha fruit pulp contains two major volatile components, limonene and g-terpinene (56.56e57.31 and 24.41e24.98%, respectively). In this study, we showed that the aroma streams of SEO, limonene, and g-terpinene exhibited significant physiological stress amelioration effects in nine healthy female panelists tested in a 30-min visual display task. While SEO administration significantly reduced work errors by 15 min compared to the odorless control condition, the limonene stream reduced work errors only at the first and fourth 5-min intervals, and the g-terpinene effect lasted only for the initial 5-min. However, g-terpinene inhalation reduced stress by significantly increasing post-work electrocardiogram ReR interval variability and lowering electroencephalogram beta wave powers at the midline occipital Oz position. Additionally, SEO, limonene, and g-terpinene displayed anti-inflammatory activity in vitro by suppressing the pro-inflammatory markers NO and IL-1b in BV- 2 microglial cells in a dose-dependent manner.

DOI： 10.38212/2224-6614.3414

Web of Science

Scopus

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.3390/molecules26247646

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1016/j.cej.2021.130502

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1016/j.sjbs.2021.08.017

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： doi:10.3390/molecules26237406

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1039/d1ra03937g

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1039/d1ra05927k

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1016/j.fitote.2021.104907

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1080/14786419.2019.1663513

記述言語：日本語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1039/d0ra06565j

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1080/14786419.2019.1616730

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1080/14786419.2019.1687477

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1177/1934578X211023363

記述言語：日本語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1080/14786419.2019.1655017

記述言語：日本語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1016/j.jchromb.2021.122555

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.13057/biodiv/d220412

記述言語：日本語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1016/j.jff.2020.104280

記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Natural Product Research  

N-[(3-(3-methyl-1-oxo-butyl)amino)propyl]-3-(3,4-dihydroxyphenyl)prop-2-enamide (7), named ‘Bassiamide A’, a new alkaloid, together with six known compounds including one lignan (1) and five lignanamides (2–6), were isolated from the aerial parts of Bassia indica Wight. The study also reported an optimal separation of a rare occurring R-isomer lignanamide derivative (6) from a natural origin, in addition to its known corresponding S-isomer (5). Structures of isolated compounds were elucidated based on NMR spectroscopic data, HR-MS, and comparison with known related ones, and they were identified as syringaresinol (1), N-trans-feruloyl-3-methoxytyramine (2), N-trans-feruloyltyramine (3), S-(-)-N-trans-feruloyl normetanephrine (4), S-(-)-N-trans-feruloyl octopamine (5), R-(+)-N-trans-feruloyl octopamine (6). The isolated compounds were evaluated for their anti-acetylcholinesterase activity, and they showed weak inhibitory activity.

DOI： 10.1080/14786419.2021.1872572

Web of Science

Scopus

PubMed

記述言語：日本語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1080/14786419.2019.1624958

記述言語：日本語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1016/j.phymed.2020.153427

記述言語：日本語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Natural Product Research  

In our promising project toward discovery of secondary metabolites with potential anticancer activity against human cervical cancer, seven marine organisms were screened for their cytotoxic activity against HeLa cancer cell line using MTT colorimetric assay. The crude extract of the outer shell of Diadema setosum showed promising activity with 88.02% inhibition at a concentration 250 µg/ml. Chromatographic investigation of the Ethyl acetate fraction, which is the main contributor to the activity (IC50= 43.1 ± 5.94 µg/ml), led to isolation of five compounds. Structures of the isolates (1-5) were elucidated by 1 D and 2 D NMR spectroscopy and HR-ESI-MS analysis. 5α,8α-epidioxycholest-6-en-3β ol (2) and 5α,8α-epidioxycholest-6,9(11)-en-3β ol (3) showed the highest cytotoxic activity with IC50 values 12.1 ± 2.74 µg/ml and 21.8 ± 6.32 µg/ml, respectively. Epidioxy steroids with cholestane nucleus could be a prospective candidate for the development of drugs for treatment of human cervical cancer.

DOI： 10.1080/14786419.2020.1855162

Web of Science

Scopus

PubMed

記述言語：日本語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1016/j.jphs.2020.08.002

記述言語：日本語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1016/j.chroma.2020.461498

記述言語：日本語   掲載種別：研究論文（学術雑誌）  

DOI： 10.13057/biodiv/d211021

記述言語：日本語   掲載種別：研究論文（学術雑誌）  

DOI： 10.17707/AgricultForest.66.3.08

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1186/s10086-020-01910-2

記述言語：日本語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1039/d0ra05265e

記述言語：日本語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1021/acsomega.0c01622

記述言語：日本語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Natural Product Research  

New cycloartane-type triterpene 23 R/S-3β-hydroxycycloart-24-ene-23-methyl ether 1a,b (as an C-23 epimeric mixture), along with ten known compounds, including 1 steroid, 3 fatty acids and 6 triterpenoids were isolated from the aerial parts of Euphorbia dendroides L. (Euphorbiaceae). The known compounds (2–11) were identified using 1 D & 2 D-NMR spectra and by comparison with data reported in the literature as well as using GC-MS for the isolated fatty acids. The new compound 1 (a,b) was elucidated by comprehensive 1 D & 2 D NMR experiments as well as LR & HR-FAB-MS. In addition, the isolated cycloartane-type triterpenoids have been tested for in vitro cytotoxicity against different cell lines. The new compound 1 (a,b) exhibited good to weak selective cytotoxic activities against HepG2, Huh-7, KLM-1, 1321N1 and HeLa cell lines with IC50 values of 20.67 ± 0.72, 16.24 ± 0.53, 22.59 ± 0.94, 25.99 ± 0.31 and 40.50 ± 3.14 μM, respectively.

DOI： 10.1080/14786419.2020.1800693

Web of Science

Scopus

PubMed

記述言語：日本語   掲載種別：研究論文（学術雑誌）  

記述言語：日本語   掲載種別：研究論文（学術雑誌）  

DOI： 10.3390/nu12061671

記述言語：日本語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1080/14786419.2018.1530231

記述言語：英語   掲載種別：研究論文（学術雑誌）  

記述言語：英語   掲載種別：研究論文（学術雑誌）  

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.25135/rnp.142.19.04.1264

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： doi:10.4005/jjfs.102.191

記述言語：英語   掲載種別：研究論文（学術雑誌）  

記述言語：日本語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1007/s00044-020-02520-9

記述言語：日本語   掲載種別：研究論文（学術雑誌）  

記述言語：日本語   掲載種別：研究論文（学術雑誌）  

記述言語：日本語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1186/s10086-020-01910-2

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： https://doi.org/10.1016/j.indcrop.2019.111778

記述言語：英語   掲載種別：研究論文（学術雑誌）  

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： https://doi.org/10.1016/j.microc.2019.103988

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： https://doi.org/10.1016/j.heliyon.2019.e02817

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1007/s11418-016-1013-1. Epub 2016 Jun 4.

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1016/j.bmc.2019.07.042

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： https://doi.org/10.1016/j.bmc.2019.07.042

記述言語：英語   掲載種別：研究論文（大学，研究機関等紀要）  

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.3390/f10090730

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1016/j.microc.2019.103988

記述言語：英語   掲載種別：研究論文（学術雑誌）  

We examined the association between serum concentrations of β-alanine, a metabolite of carnosine and anserine, and the risk of dementia
in a general population of elderly Japanese persons. In 2007, 1,475 residents of Hisayama, Japan, aged 60–79 years and without dementia were divided into 4 groups according to quartiles of serum β-alanine concentrations (quartile 1, lowest; quartile 4, highest) and followed for a median of 5.3 years. During follow-up, 117 subjects developed all-cause dementia (Alzheimer in 77 cases and vascular dementia in 31).
The risk of all-cause dementia decreased with increasing serum β-alanine levels after adjustment for potential confounding factors
(quartile 2, hazard ratio (HR) = 0.73 (95% confidence interval (CI): 0.45, 1.18); quartile 3, HR = 0.50 (95% CI: 0.28, 0.89); quartile 4,
HR = 0.50 (95% CI: 0.27, 0.92); P = 0.01 for trend). A similar inverse association was observed for Alzheimer disease (quartile 2, HR = 0.78 (95% CI: 0.44, 1.38); quartile 3, HR = 0.53 (95% CI: 0.26, 1.06); quartile 4, HR = 0.53 (95% CI: 0.25, 1.10); P = 0.04 for trend)
but not for vascular dementia. We found that higher serum β-alanine levels were significantly associated with lower risks of all-cause dementia and Alzheimer disease. Because serum β-alanine levels reflect intakes of carnosine/anserine, higher intakes of carnosine/anserine might be beneficial for the prevention of dementia.

DOI： https://doi.org/10.1093/aje/kwz116

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1016/j.phytol.2019.04.017

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1002/cbdv.201900237

記述言語：日本語   掲載種別：研究論文（学術雑誌）  

記述言語：日本語   掲載種別：研究論文（学術雑誌）  

記述言語：英語   掲載種別：研究論文（学術雑誌）  

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1016/j.jff.2019.04.058

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1177/1934578X19867192

記述言語：日本語   掲載種別：研究論文（学術雑誌）  

記述言語：日本語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1093/chromsci/bmz020

記述言語：日本語   掲載種別：研究論文（学術雑誌）  

As a construction material, Sugi (Cryptomeria japonica) is widely used in Japan. Present study focused on how wooden dwelling spaces with Sugi wood material influence quality of human sleep. To clarify this, Room A where naturally processed Sugi wood boards were used for the interiors and the Room B where chemically processed wood materials comprised of bonded or coated woods were used for the interiors were investigated for comparison. Volatile organic compounds (mainly sesqui-terpenes) emitted from both rooms were analyzed by Gas Chromatography/Mass Spectrometry. Ten participants were asked to take a sleep in the two rooms, respectively. Salivary stress markers, sleep time, blood pressure, questionnaires were measured. Humidity and temperature of the both rooms were recorded. The participants’ lifestyles were also investigated. Compared to several characters of Room B, humidity of Room A was lower, and concentration of sesqui-terpenes emitted from Room A were higher. Participants felt more “natural” in Room A. Participant with poor daily sleep quality showed higher morning diastolic pressure only when participants slept in Room B. These results indicate that, high emitting of sesqui-terpenes, high moisture-absorption performance or the combination of them of naturally processed Sugi wood can help people prevent diastolic pressure increasing in the morning caused by low sleep quality.

記述言語：英語   掲載種別：研究論文（学術雑誌）  

Skeletal muscle fiber is largely classified into two types: type 1 (slow‐twitch) and type 2 (fast‐twitch) fibers. Meat quality and composition of fiber types are thought to be closely related. Previous research showed that overexpression of constitutively active peroxisome proliferator‐activated receptor (PPAR)δ, a nuclear receptor present in skeletal muscle, increased type 1 fibers in mice. In this study, we found that hexane extracts of Yamabushitake mushroom (Hericium erinaceus) showed PPARδ agonistic activity in vitro. Eight‐week‐old C57BL/6J mice were fed a diet supplemented with 5% (w/w) freeze‐dried Yamabushitake mushroom for 24 hr. After the treatment period, the extensor digitorum longus (EDL) muscles were excised. The Yamabushitake‐supplemented diet up‐regulated the PPARδ target genes Pdk4 and Ucp3 in mouse skeletal muscles in vivo. Furthermore, feeding the Yamabushitake‐supplemented diet to mice for 8 weeks resulted in a significant increase in muscle endurance. These results indicate that Yamabushitake mushroom contains PPARδ agonistic ligands and that dietary intake of Yamabushitake mushroom could activate PPARδ in skeletal muscle of mice. Unexpectedly, we observed no significant alterations in composition of muscle fiber types between the mice fed control and Yamabushitake‐supplemented diets.

DOI： 10.1111/asj.13199

記述言語：英語   掲載種別：研究論文（学術雑誌）  

記述言語：日本語   掲載種別：研究論文（学術雑誌）  

記述言語：英語   掲載種別：研究論文（学術雑誌）  

This study investigated in vitro the α-glucosidase inhibitory activity of resin from the Sakhalin fir tree (Abies sachalinensis). The resin showed extremely high activity (IC50 of 17.3 µg/mL). We isolated 8 compounds from the resin and identified them. All of the compounds isolated from A. sachalinensis resin are reported for the first time in the present study. In an α-glucosidase inhibitory assay, 6 compounds—(holophyllane C (1), (−) -(24 E)-23-oxo-3,4- seco-9βH-lanosta-4(28),6,8(14),24-tetraen-3,26-dioic acid (2), abiesonic acid (3), (−)-(24 E)-23-oxo-3,4- seco-9β H-lanosta-4(28),7,24-triene-3,26-dioic acid (4), 3,4-seco-4(28),6,8(14),22Z,24-mariesapentaen-26,23-olide-3-oic acid (5), and (−)-abiesonic acid 3-methyl ester (6))—showed high activity (their IC50 values were 25.1, 20.9, 82.4, 20.5, 27.3, and 20.5 µg/mL, respectively). These compounds should contribute to the resin’s α-glucosidase inhibitory activity. From an industrial point of view, these findings provide new data to support that the resin of A. sachalinensis is a rich source of α-glucosidase inhibitors, which are highly valuable for their potential to be developed as functional health foods.

DOI： 10.1177/1934578X19858460

記述言語：日本語   掲載種別：研究論文（学術雑誌）  

記述言語：英語   掲載種別：研究論文（学術雑誌）  

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： https://doi.org/10.1080/14786419.2019.1616730

記述言語：英語   掲載種別：研究論文（学術雑誌）  

Lingzhi is a Ganoderma mushroom species which has a wide range of bioactivities. Analysis of the changes in metabolites during the developmental stages of lingzhi is important to understand the underlying mechanism of its biosynthesis, as well as its bioactivity. It may also provide valuable information for the cultivation efficiency of lingzhi. In this study, mass spectrometry based untargeted metabolomics was carried out to analyze the alteration of metabolites during developmental stages of lingzhi. Eight developmental stages were categorized on the basis of morphological changes; starting from mycelium stage to post-mature stage. GC/MS and LC/MS analyses along with multivariate analysis of lingzhi developmental stages were performed. Amino acids, organic acids, sugars, polyols, fatty acids, fatty alcohols, and some small polar metabolites were extracted as marker metabolites from GC/MS analysis, while, lanostane-type triterpenoids were observed in LC/MS analysis of lingzhi. The marker metabolites from untargeted analysis of lingzhi developmental stages were correlated with the α-glucosidase inhibitory activity. Two metabolites, compounds 34 and 35, were identified as potential contributors of the α-glucosidase inhibitory activity. The current result shows that some metabolites are involved in the developmental process and α-glucosidase inhibitory activity of lingzhi.

DOI： 10.3390/molecules24112044.

記述言語：英語   掲載種別：研究論文（学術雑誌）  

We examined the association between serum concentrations of β-alanine, a metabolite of carnosine and anserine, and the risk of dementia in a general population of elderly Japanese persons. In 2007, 1,475 residents of Hisayama, Japan, aged 60–79 years and without dementia were divided into 4 groups according to quartiles of serum β-alanine concentrations (quartile 1, lowest; quartile 4, highest) and followed for a median of 5.3 years. During follow-up, 117 subjects developed all-cause dementia (Alzheimer in 77 cases and vascular dementia in 31).The risk of all-cause dementia decreased with increasing serum β-alanine levels after adjustment for potential confounding factors (quartile 2, hazard ratio (HR) = 0.73 (95% confidence interval (CI): 0.45, 1.18); quartile 3, HR =0.50 (95% CI: 0.28, 0.89); quartile 4, HR = 0.50 (95% CI: 0.27, 0.92); P = 0.01 for trend). A similar inverse association was observed for Alzheimer disease (quartile 2, HR = 0.78 (95% CI: 0.44, 1.38); quartile 3, HR = 0.53 (95% CI:0.26, 1.06); quartile 4, HR = 0.53 (95% CI: 0.25, 1.10); P = 0.04 for trend) but not for vascular dementia. We found that higher serum β-alanine levels were significantly associated with lower risks of all-cause dementia and Alzheimer disease. Because serum β-alanine levels reflect intakes of carnosine/anserine, higher intakes of carnosine/anserine might be beneficial for the prevention of dementia.β-alanine; Alzheimer disease; cohort study; dementia; imidazole dipeptides

DOI： 10.1093/aje/kwz116

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： https://doi.org/10.1080/02773813.2019.1578806

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1021/acs.jafc.8b06954

記述言語：日本語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1089/jmf.2018.4267

記述言語：英語   掲載種別：研究論文（学術雑誌）  

Two new flavonoid glycosides, kaempferol 3-O-α-L-rhamnopyranosyl (1→6) (3′′-acetyl)-β-D-galactopyranoside 1 and kaempferol 3-O-α-L-arabinopyranosyl-5-O-α-L-rhamnopyranoside 2, along with six known ones 3–8 were isolated from the flowers of Vicia faba L. (Fabaceae). Methanol extract and the isolated compounds were tested against lipase and melanogenesis inhibition activities and resulted in that compound 2 showed 53 and 77% lipase inhibition activity in concentrations of 400 and 800 μg/mL, respectively. For melanogenesis, compounds 2, 3 and 4 exhibited potent melanogenesis inhibition activity where the melanin content in melanoma cells was decreased to be about 57.5, 56 and 61%, respectively, with no obvious melanocytotoxicity. The rest of compounds showed weak to moderate activity. The results of melanogenesis inhibition activity of this study suggested the potential use of Vicia faba flowers as a skin-whitening agent and reveal the flowers to be a rich source of important phytochemicals with antilipase and melanogenesis inhibitory activity.

記述言語：英語   掲載種別：研究論文（学術雑誌）  

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1080/14786419.2018.1440229

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1089/jmf.2018.4267

記述言語：英語   掲載種別：研究論文（学術雑誌）  

記述言語：英語   掲載種別：研究論文（学術雑誌）  

Lingzhi is a Ganoderma mushroom species which has a wide range of bioactivities. Analysis of the changes in metabolites during the developmental stages of lingzhi is important to understand the underlying mechanism of its biosynthesis, as well as its bioactivity. It may also provide valuable information for the cultivation efficiency of lingzhi. In this study, mass spectrometry based untargeted metabolomics was carried out to analyze the alteration of metabolites during developmental stages of lingzhi. Eight developmental stages were categorized on the basis of morphological changes; starting from mycelium stage to post-mature stage. GC/MS and LC/MS analyses along with multivariate analysis of lingzhi developmental stages were performed. Amino acids, organic acids, sugars, polyols, fatty acids, fatty alcohols, and some small polar metabolites were extracted as marker metabolites from GC/MS analysis, while, lanostane-type triterpenoids were observed in LC/MS analysis of lingzhi. The marker metabolites from untargeted analysis of lingzhi developmental stages were correlated with the α-glucosidase inhibitory activity. Two metabolites, compounds 34 and 35, were identified as potential contributors of the α-glucosidase inhibitory activity. The current result shows that some metabolites are involved in the developmental process and α-glucosidase inhibitory activity of lingzhi.

DOI： 10.3390/molecules24112044

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.2220/biomedres.40.125

記述言語：英語   掲載種別：研究論文（学術雑誌）  

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1080/02773813.2019.1578806

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1080/09540105.2019.1651255

記述言語：英語   掲載種別：研究論文（学術雑誌）  

A new glucoside, 3-methoxy-4-O-β-D-glucopyranosyl-methyl benzoate, has been isolated from Lycium schweinfurthii along with five known compounds through bioactivity guided fractionation of the total plant methanolic extract towards α-glucosidase inhibitory activity. All the isolated compounds were tested for their inhibitory effect on α-glucosidase enzyme. As a result, four of them showed a potent inhibitory activity and thus constitute a therapeutic approach to decrease postprandial hyperglycemia in diabetic patients.

DOI： 10.1080/14786419.2019.1616730

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1093/chromsci/bmz020

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1080/14786419.2019.1624958.

記述言語：英語   掲載種別：研究論文（学術雑誌）  

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1007/s13196-018-0215-4

記述言語：日本語   掲載種別：研究論文（学術雑誌）  

記述言語：英語   掲載種別：研究論文（学術雑誌）  

記述言語：日本語   掲載種別：研究論文（学術雑誌）  

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1080/14786419.2017.1419234

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1016/j.exppara.2018.10.003. Epub 2018 Oct 16.

記述言語：日本語   掲載種別：研究論文（学術雑誌）  

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1021/acs.jafc.8b03648

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： https://doi.org/10.1016/j.jfda.2017.12.008

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1007/s10616-018-0229-1

記述言語：英語   掲載種別：研究論文（学術雑誌）  

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1016/j.phytochem.2018.02.006

記述言語：英語   掲載種別：研究論文（学術雑誌）  

記述言語：日本語   掲載種別：研究論文（学術雑誌）  

This study focused on the humidity-control performance of the wooden dwelling spaces, which is related to the habitability. To evaluate the moisture-absorption performance in a condition close to real life, the room temperature and relative humidity were measured in a room using naturally processed sugi （Cryptomeria japonica ） wood boards （Room A） and a room using chemically processed wood materials（Room B）as interior materials while subjects were asleep. The interior materials of Room A were free from chemical processing and those of Room B were comprised of bonded or coated woods.
The results obtained from this study during 2014 and 2015 demonstrated that the Room A showed
higher moisture-absorption performance than the Room B.

リポジトリ公開URL： http://hdl.handle.net/2324/1932323

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1016/j.jfda.2017.06.005

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1007/s11418-018-1213-y

記述言語：英語   掲載種別：研究論文（学術雑誌）  

Three spectrofluorimetric methods were developed for agomelatine (AGM) determination in commercial tablets. Method A is based on measuring the native fluorescence of AGM aqueous solution at 230/360 nm. Methods B and C are based on the formation of a charge transfer complex between AGM and 2,3-dichloro-5,6-dicyano-1,4-benzoquinone (DDQ) and 7,7,8,8-tetracyanoquinodimethane (TCNQ) with measurement of the formed fluorophore at 365/475 nm and 250/304 nm, respectively. The relative fluorescence intensity (RFI) of AGM–DDQ complex was greatly enhanced in the presence of methyl-β-cyclodextrin (CD). The methods were linear over the concentration ranges of 0.015–0.5, 0.5–8.0, 0.09–6.0 and 0.05–0.2 μg/ml for AGM-native fluorescence, AGM–DDQ, AGM–DDQ–CD and AGM–TCNQ complexes, respectively with excellent correlation coefficients (r = 0.9999). The methods were validated as per the International Conference on Harmonization (ICH) guidelines and all validation requirements were satisfied. The developed methods were extended to the analysis of AGM in commercial tablets. Furthermore, the stability of AGM was studied under different stress conditions (alkaline, acidic, oxidative and photolytic). The potential alkaline and acidic degradation products were identified by LC–MS/TOF.

DOI： 10.1002/bio.3405

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1080/14786419.2018.1440229

記述言語：日本語   掲載種別：研究論文（学術雑誌）  

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.22146/ijc.25467

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1016/j.intimp.2017.12.023

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1055/s-0044-100797

記述言語：英語   掲載種別：研究論文（学術雑誌）  

The investigation of the Syzygium polycephalum Miq. (Myrtaceae) aimed to assess the phytochemical contents and antioxidant activity of the chloroform fraction. In this study, the fraction was obtained from methanol extract of S. polycephalum stem bark partitionated by chloroform. An ellagic acid derivative was successively isolated from the chloroform fraction. The molecular structure of isolated compound was elucidated and established as 3,4,3’-tri-O-methylellagic acid through extensive spectroscopic studies including UV-Vis, FTIR, NMR and LC-MS analyses and by comparison with literature data. The finding of the isolated compound is the first time from the plant, although the isolated compound previously have been found in the other Syzygium species such as S. cumini together with ellagic acid and 3,3’-di-O-methylellagic acid. The chloroform fraction, isolated compound, and vitamin C showed antioxidant activity against 2,2’-diphenyl-1-picrylhydrazyl (DPPH) with IC50 value of 163.6, 72.1, and 11.5 μg/mL, respectively.

記述言語：英語   掲載種別：研究論文（学術雑誌）  

記述言語：英語   掲載種別：研究論文（学術雑誌）  

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1016/j.jfda.2017.12.008

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1080/14786419.2017.1419234

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1080/14786419.2017.1292270

記述言語：英語   掲載種別：研究論文（学術雑誌）  

記述言語：英語   掲載種別：研究論文（学術雑誌）  

Three spectrofluorimetric methods were developed for agomelatine (AGM) determination in commercial tablets. Method A is based on measuring the native fluorescence of AGM aqueous solution at 230/360 nm. Methods B and C are based on the formation of a charge transfer complex between AGM and 2,3-dichloro-5,6-dicyano-1,4-benzoquinone (DDQ) and 7,7,8,8-tetracyanoquinodimethane (TCNQ) with measurement of the formed fluorophore at 365/475 nm and 250/304 nm, respectively. The relative fluorescence intensity (RFI) of AGM-DDQ complex was greatly enhanced in the presence of methyl-β-cyclodextrin (CD). The methods were linear over the concentration ranges of 0.015-0.5, 0.5-8.0, 0.09-6.0 and 0.05-0.2 μg/ml for AGM-native fluorescence, AGM-DDQ, AGM-DDQ-CD and AGM-TCNQ complexes, respectively with excellent correlation coefficients (r = 0.9999). The methods were validated as per the International Conference on Harmonization (ICH) guidelines and all validation requirements were satisfied. The developed methods were extended to the analysis of AGM in commercial tablets. Furthermore, the stability of AGM was studied under different stress conditions (alkaline, acidic, oxidative and photolytic). The potential alkaline and acidic degradation products were identified by LC-MS/TOF.

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1007/s10753-017-0563-z

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1080/14786419.2017.1359169

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1007/s10086-017-1636-1

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1016/j.jfda.2016.09.009

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1080/14786419.2017.1354183

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1007/s11418-017-1075-8

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1016/j.jff.2017.04.040

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.3390/ijms18051030

記述言語：英語   掲載種別：研究論文（学術雑誌）  

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1007/s10616-016-0058-z

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1007/s11418-017-1072-y

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1007/s11418-016-1069-y

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1007/s00284-016-1184-8

記述言語：日本語   掲載種別：研究論文（学術雑誌）  

記述言語：英語  

記述言語：日本語   掲載種別：研究論文（学術雑誌）  

記述言語：日本語   掲載種別：研究論文（学術雑誌）  

記述言語：日本語   掲載種別：研究論文（学術雑誌）  

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.22146/ijc.23545

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1093/chromsci/bmw143

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.4172/pharmaceutical-sciences.1000265

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1177/1076029615595879

記述言語：英語  

記述言語：英語  

記述言語：英語   掲載種別：研究論文（学術雑誌）  

記述言語：英語   掲載種別：研究論文（学術雑誌）  

記述言語：英語  

記述言語：英語   掲載種別：研究論文（学術雑誌）  

記述言語：英語  

記述言語：英語  

記述言語：英語  

記述言語：英語   掲載種別：研究論文（学術雑誌）  

Moringa oleifera have been evaluated for its antioxidant activity. M. oleifera leaves were extracted with methanol, ethyl acetate, dichloromethane and n-hexane. The antioxidant activity of extracts were evaluated by 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity assay and an improved 2,2’-azino-bis-[3-ethylbenzothiazoline sulphonate] (ABTS) radical cation decolorization assay in vitro. Trolox was used as standard with IC50 5.89 μg/mL in DPPH assay and 3.06 μg/mL in ABTS assay. The methanol extract showed the highest free radical scavenging activity with IC50 value of 49.30 μg/mL in DPPH assay and 11.73 μg/mL in ABTS assay. This study provided that M. oleifera leaves possess antioxidant.

DOI： https://doi.org/10.22146/ijc.21145

記述言語：英語   掲載種別：研究論文（学術雑誌）  

記述言語：英語   掲載種別：研究論文（学術雑誌）  

記述言語：英語   掲載種別：研究論文（学術雑誌）  

記述言語：英語   掲載種別：研究論文（学術雑誌）  

記述言語：英語   掲載種別：研究論文（学術雑誌）  

記述言語：英語   掲載種別：研究論文（学術雑誌）  

記述言語：英語   掲載種別：研究論文（学術雑誌）  

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1039/C5RA26424C

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1039/C5AY03003J

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： DOI:10.1080/02773813.2015.1057648

記述言語：日本語   掲載種別：研究論文（学術雑誌）  

記述言語：日本語   掲載種別：研究論文（学術雑誌）  

記述言語：日本語   掲載種別：研究論文（大学，研究機関等紀要）  

記述言語：日本語   掲載種別：研究論文（大学，研究機関等紀要）  

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1055/s-0042-104202

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1007/s10616-015-9849-x

記述言語：日本語   掲載種別：研究論文（大学，研究機関等紀要）  

記述言語：日本語   掲載種別：研究論文（大学，研究機関等紀要）  

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： doi: 10.1177/1076029615595879

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： doi:10.1016/j.phytol.2015.04.021

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1016/j.phytol.2015.04.021

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： DOI: 10.1002/ptr.5326

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： e0128358

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： DOI: 10.1038/srep13194.

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1016/j.phymed.2015.03.002

記述言語：英語  

記述言語：英語  

記述言語：英語  

DOI： 10.3390/molecules20034813

記述言語：英語   掲載種別：研究論文（学術雑誌）  

記述言語：英語   掲載種別：研究論文（学術雑誌）  

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： https://doi.org/10.1016/j.biortech.2014.05.064

記述言語：英語  

記述言語：英語  

記述言語：英語  

DOI： 10.1016/j.bmcl.2014.08.009

記述言語：英語  

DOI： doi:10.3390/molecules190913473

記述言語：英語   掲載種別：研究論文（学術雑誌）  

記述言語：英語  

DOI： 10.3390/molecules19068238

記述言語：英語  

記述言語：英語  

記述言語：英語  

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： doi: 10.1021/jf402735u

記述言語：日本語   掲載種別：研究論文（学術雑誌）  

記述言語：英語   掲載種別：研究論文（学術雑誌）  

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： http://dx.doi.org/10.1155/2014/195305

記述言語：英語  

DOI： doi: 10.1016/j.bmc.2013.11.034.

記述言語：英語  

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1016/j.bmcl.2013.08.084

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： doi:10.3390/molecules181113823

記述言語：英語  

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： Volume 2014 (2014), Article ID 195305, 11 pages http://dx.doi.org/10.1155/2014/195305

記述言語：英語   掲載種別：研究論文（学術雑誌）  

記述言語：英語   掲載種別：研究論文（学術雑誌）  

記述言語：英語   掲載種別：研究論文（学術雑誌）  

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： doi:10.1038/protex.2013.051

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1007/s10086-012-1310-6

記述言語：英語   掲載種別：研究論文（学術雑誌）  

記述言語：英語   掲載種別：研究論文（学術雑誌）  

記述言語：日本語   掲載種別：研究論文（学術雑誌）  

記述言語：日本語   掲載種別：研究論文（大学，研究機関等紀要）  

記述言語：日本語   掲載種別：研究論文（学術雑誌）  

記述言語：日本語   掲載種別：研究論文（大学，研究機関等紀要）  

記述言語：英語   掲載種別：研究論文（学術雑誌）  

記述言語：英語   掲載種別：研究論文（学術雑誌）  

記述言語：英語   掲載種別：研究論文（学術雑誌）  

記述言語：英語   掲載種別：研究論文（学術雑誌）  

記述言語：英語   掲載種別：研究論文（学術雑誌）  

記述言語：英語   掲載種別：研究論文（学術雑誌）  

記述言語：英語   掲載種別：研究論文（学術雑誌）  

記述言語：英語   掲載種別：研究論文（学術雑誌）  

記述言語：英語   掲載種別：研究論文（学術雑誌）  

記述言語：英語   掲載種別：研究論文（学術雑誌）  

記述言語：英語   掲載種別：研究論文（学術雑誌）  

A large quantity of quercetin was characterized from a local dish, known as maniçoba in Pará, Brazil. The fresh leaf of Manihot esculenta Crantz (Euphorbiaceae), a principal ingredient for maniçoba, was found to contain a large amount of rutin or quercetin 3-O-rutinoside which was completely hydrolyzed to quercetin during cooking processes. Antioxidant activity of quercetin and its microfloral metabolites was examined. Quercetin inhibited the oxidation of linoleic acid catalyzed by soybean lipoxygenase-1 (EC 1.13.11.12, Type 1) with an IC₅₀of 4.5 μM, while rutin did not inhibit this oxidation up to 200 μM. The progress curves for the enzyme reaction indicate that quercetin shows slow binding kinetics. In the pseudoperoxidase activity, hydroperoxides slowly oxidize quercetin to a rather stable intermediate,
2-(3,4-dihydroxybenzoyl)-2,4,6-trihydroxy-3(2H)-benzofuranone. A microfloral metabolite of quercetin and rutin after ingestion, 3,4-dihydroxyphenylacetic acid, also scavenged superoxide anion generated by xanthine oxidase and inhibited uric acid formation. The antioxidant activity observed with the methanol extract of maniçoba is due mainly to multifunctional quercetin's response.

記述言語：日本語   掲載種別：研究論文（学術雑誌）  

記述言語：英語   掲載種別：研究論文（学術雑誌）  

記述言語：英語   掲載種別：研究論文（学術雑誌）  

記述言語：英語   掲載種別：研究論文（学術雑誌）  

記述言語：英語   掲載種別：研究論文（学術雑誌）  

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： https://doi.org/10.1177/1934578X1100600943

記述言語：英語   掲載種別：研究論文（学術雑誌）  

記述言語：英語  

記述言語：英語   掲載種別：研究論文（学術雑誌）  

記述言語：日本語   掲載種別：研究論文（学術雑誌）  

記述言語：英語   掲載種別：研究論文（学術雑誌）  

記述言語：英語  

記述言語：英語   掲載種別：研究論文（学術雑誌）  

記述言語：英語   掲載種別：研究論文（学術雑誌）  

記述言語：英語  

記述言語：英語   掲載種別：研究論文（学術雑誌）  

記述言語：日本語   掲載種別：研究論文（学術雑誌）  

記述言語：日本語   掲載種別：研究論文（学術雑誌）  

記述言語：英語   掲載種別：研究論文（学術雑誌）  

記述言語：英語   掲載種別：研究論文（学術雑誌）  

記述言語：日本語   掲載種別：研究論文（学術雑誌）  

記述言語：英語   掲載種別：研究論文（学術雑誌）  

記述言語：英語   掲載種別：研究論文（学術雑誌）  

記述言語：英語   掲載種別：研究論文（学術雑誌）  

記述言語：英語   掲載種別：研究論文（学術雑誌）  

記述言語：英語  

記述言語：日本語   掲載種別：研究論文（学術雑誌）  

記述言語：英語   掲載種別：研究論文（学術雑誌）  

記述言語：英語  

記述言語：日本語   掲載種別：研究論文（学術雑誌）  

記述言語：日本語   掲載種別：研究論文（学術雑誌）  

記述言語：英語  

記述言語：英語   掲載種別：研究論文（学術雑誌）  

記述言語：英語   掲載種別：研究論文（学術雑誌）  

記述言語：英語   掲載種別：研究論文（学術雑誌）  

記述言語：英語   掲載種別：研究論文（学術雑誌）  

記述言語：英語   掲載種別：研究論文（学術雑誌）