Language：English   Publishing type：Research paper (scientific journal)  

Biosynthetic pathway of beta-thujaplicin in the Cupressus lusitanica cell culture

Language：English   Publishing type：Research paper (scientific journal)  

Cationic cell-wall-bound peroxidase (CWPO-C) from Populus alba is the only Class III peroxidase that has been shown to be able to oxidize high molecular weight lignin polymers from sinapyl alcohol and previously, has been believed to be a lignin polymerization-specific peroxidase. However, using an Arabidopsis heterologous expression system, we showed recently that CWPO-C contributes to differentiation or early growth and is involved in auxin catabolism. In this study, to clarify the function of CWPO-C in poplar, we analyzed CWPO-C gene expression and phenotypic changes with CWPO-C overexpression and suppression. Real-time PCR and monitoring promoter activity of CWPO-C using β-glucuronidase (GUS) assay revealed that CWPO-C was strongly expressed in immature tissues, such as the upper stem, axillary buds, and young leaves, in addition to expression in developing xylem. In transgenic poplars in which the expression of CWPO-C was upregulated or suppressed, changes in stem growth, gravitropism bending time, lignin content and syringyl/guaiacyl (S/G) composition were observed. Overexpressing CWPO-C enhanced stem growth and gravitropic response (shorter bending time). With suppressed CWPO-C expression, the lignin content was reduced approximately 45% and the S/G ratio decreased by half. These results strongly suggest that CWPO-C plays a role in differentiation and early growth, as well as in lignin polymerization.

DOI： https://doi.org/10.1186/s10086-023-02086-1

Publishing type：Research paper (scientific journal)   Publisher：Journal of Wood Science  

Cationic cell-wall-bound peroxidase (CWPO-C) from Populus alba is the only Class III peroxidase that has been shown to be able to oxidize high molecular weight lignin polymers from sinapyl alcohol and previously, has been believed to be a lignin polymerization-specific peroxidase. However, using an Arabidopsis heterologous expression system, we showed recently that CWPO-C contributes to differentiation or early growth and is involved in auxin catabolism. In this study, to clarify the function of CWPO-C in poplar, we analyzed CWPO-C gene expression and phenotypic changes with CWPO-C overexpression and suppression. Real-time PCR and monitoring promoter activity of CWPO-C using β-glucuronidase (GUS) assay revealed that CWPO-C was strongly expressed in immature tissues, such as the upper stem, axillary buds, and young leaves, in addition to expression in developing xylem. In transgenic poplars in which the expression of CWPO-C was upregulated or suppressed, changes in stem growth, gravitropism bending time, lignin content and syringyl/guaiacyl (S/G) composition were observed. Overexpressing CWPO-C enhanced stem growth and gravitropic response (shorter bending time). With suppressed CWPO-C expression, the lignin content was reduced approximately 45% and the S/G ratio decreased by half. These results strongly suggest that CWPO-C plays a role in differentiation and early growth, as well as in lignin polymerization.

DOI： 10.1186/s10086-023-02086-1

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Language：English   Publisher：Physiology and Molecular Biology of Plants  

In this article the family name of the fifth author Tomiie was incorrectly written as Tomie. The correct name should read as Azusa Tomiie. The original article has been corrected.

DOI： 10.1007/s12298-022-01259-4

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Language：English   Publishing type：Research paper (scientific journal)  

The poplar cationic cell-wall-bound peroxidase (CWPO-C) mediates the oxidative polymerization of lignin precursors, especially sinapyl alcohols, and high molecular weight compounds that cannot be oxidized by other plant peroxidases, including horseradish peroxidase C. Therefore, CWPO-C is believed to be a lignification-specific peroxidase, but direct evidence of its function is lacking. Thus, the CWPO-C expression pattern in Arabidopsis thaliana (Arabidopsis) was determined using the β-glucuronidase gene as a reporter. Our data indicated that CWPO-C was expressed in young organs, including the meristem, leaf, root, flower, and young xylem in the upper part of the stem. Compared with the wild-type control, transgenic Arabidopsis plants overexpressing CWPO-C had shorter stems. Approximately 60% of the plants in the transgenic line with the highest CWPO-C content had curled stems. These results indicate that CWPO-C plays a role in cell elongation. When plants were placed horizontally, induced CWPO-C expression was detected in the curved part of the stem during the gravitropic response. The stem curvature associated with gravitropism is controlled by auxin localization. The time needed for Arabidopsis plants overexpressing CWPO-C placed horizontally to bend by 90° was almost double the time required for the similarly treated wild-type controls. Moreover, the auxin content was significantly lower in the CWPO-C-overexpressing plants than in the wild-type plants. These results strongly suggest that CWPO-C has pleiotropic effects on plant growth and indole-3-acetic acid (IAA) accumulation. These effects may be mediated by altered IAA concentration due to oxidation.

DOI： doi.org/10.1007/s12298-022-01241-0

Language：English   Publishing type：Research paper (scientific journal)   Publisher：SPRINGER  

The poplar cationic cell-wall-bound peroxidase (CWPO-C) mediates the oxidative polymerization of lignin precursors, especially sinapyl alcohols, and high molecular weight compounds that cannot be oxidized by other plant peroxidases, including horseradish peroxidase C. Therefore, CWPO-C is believed to be a lignification-specific peroxidase, but direct evidence of its function is lacking. Thus, the CWPO-C expression pattern in Arabidopsis thaliana (Arabidopsis) was determined using the beta-glucuronidase gene as a reporter. Our data indicated that CWPO-C was expressed in young organs, including the meristem, leaf, root, flower, and young xylem in the upper part of the stem. Compared with the wild-type control, transgenic Arabidopsis plants overexpressing CWPO-C had shorter stems. Approximately 60% of the plants in the transgenic line with the highest CWPO-C content had curled stems. These results indicate that CWPO-C plays a role in cell elongation. When plants were placed horizontally, induced CWPO-C expression was detected in the curved part of the stem during the gravitropic response. The stem curvature associated with gravitropism is controlled by auxin localization. The time needed for Arabidopsis plants overexpressing CWPO-C placed horizontally to bend by 90 degrees was almost double the time required for the similarly treated wild-type controls. Moreover, the auxin content was significantly lower in the CWPO-C-overexpressing plants than in the wild-type plants. These results strongly suggest that CWPO-C has pleiotropic effects on plant growth and indole-3-acetic acid (IAA) accumulation. These effects may be mediated by altered IAA concentration due to oxidation.

DOI： 10.1007/s12298-022-01241-0

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Language：English   Publishing type：Research paper (scientific journal)  

The mechanism of lignin dehydrogenative polymerization (DHP), made by means of horseradish peroxidase (HRP), was studied in comparison with other plant peroxidases. Interestingly, HRP is efficient for guaiacyl type polymer formation (G-DHPs), but is not efficient in the case of syringyl type DHPs (S-DHPs). It was previously demonstrated that lignification-related Arabidopsis thaliana peroxidases, AtPrx2, AtPrx25 and AtPrx71, and cationic cell-wall-bound peroxidase (CWPO-C) from Populus alba are successful to oxidize syringyl- and guaiacyl-type monomers and larger lignin-like molecules. This is the reason why in the present study the DHP formation by means of these recombinant peroxidases was tested, and all these enzymes were successful for formation of both G-DHP and S-DHP in acceptable yields. CWPO-C led to S-DHP molecular size distribution similar to that of isolated lignins.

DOI： 10.1515/hf-2017-0125

Language：Japanese   Publishing type：Research paper (scientific journal)  

Analysis of volatile compounds and their seasonal changes in room using Sugi (Cryptomeria japonica) wood boards as interior materials

Language：English   Publishing type：Research paper (scientific journal)  

Most of the known 4-coumarate:coenzyme A ligase (4CL) isoforms lack CoA-ligation activity for sinapic acid. Therefore, there is some doubt as to whether sinapic acid contributes to sinapyl alcohol biosynthesis. In this study, we characterized the enzyme activity of a protein mixture extracted from the developing xylem of Robinia pseudoacacia. The crude protein mixture contained at least two 4CLs with sinapic acid 4-CoA ligation activity. The crude enzyme preparation displayed negligible sinapaldehyde dehydrogenase activity, but showed ferulic acid 5-hydroxylation activity and 5-hydroxyferulic acid O-methyltransferase activity; these activities were retained in the presence of competitive substrates (coniferaldehyde and 5-hydroxyconiferaldehyde, respectively). 5-Hydroxyferulic acid and sinapic acid accumulated in the developing xylem of R. pseudoacacia, suggesting, in part at least, sinapic acid is a sinapyl alcohol precursor in this species.

DOI： 10.1007/s10265-016-0882-4

Language：English   Publishing type：Research paper (scientific journal)  

Plant class III heme peroxidases catalyze lignin polymerization. Previous reports have shown that at least three Arabidopsis thaliana peroxidases, AtPrx2, AtPrx25 and AtPrx71, are involved in stem lignification using T-DNA insertion mutants, atprx2, atprx25, and atprx71. Here, we generated three double mutants, atprx2/atprx25, atprx2/atprx71, and atprx25/atprx71, and investigated the impact of the simultaneous deficiency of these peroxidases on lignins and plant growth. Stem tissue analysis using the acetyl bromide method and derivatization followed by reductive cleavage revealed improved lignin characteristics, such as lowered lignin content and increased arylglycerol-β-aryl (β-O-4) linkage type, especially β-O-4 linked syringyl units, in lignin, supporting the roles of these genes in lignin polymerization. In addition, none of the double mutants exhibited severe growth defects, such as shorter plant stature, dwarfing, or sterility, and their stems had improved cell wall degradability. This study will contribute to progress in lignin bioengineering to improve lignocellulosic biomass.

DOI： 10.1111/jipb.12334

Language：English   Publishing type：Research paper (scientific journal)  

We showed previously that in Cupressus lusitanica (Mexican cypress), the first two steps of terpinolene oxidation, beginning at hydroxylation at the aryl position and then forming epoxide at the double bond, were driven by cytochrome P450s. The significance of enantio differences, in general, has received attention because those enantiomers often have their own biological activities. We, therefore, investigated the stereo-specificity of the substrate and the enantio-selectivity of these reactions. The hydroxylation of terpinolene by cytochrome P450 in the microsomal fraction from C. lusitanica cells gave a single product with an S configuration of 5-isopropylidene-2-methylcyclohex-2-enol. Next, epoxidizing enzyme accepted only the S-configuration substrate and produced a single enantiomer product, (1R, 2S, 6S)-(+)-1,6-epoxy-4(8)-p-menthen-2-ol. No isomer was detected at our gas chromatography/mass spectrometry sensitivity; therefore, the calculated enantiomeric excess values were 100 %. These results indicate that the cytochrome P450s involved in terpinolene metabolism in C. lusitanica cells have very strict stereo-selective ability. Our findings may be helpful in the stereo-selective synthesis of fine chemicals, although the physiological meanings of these chiral products are still not unclear.

DOI： 10.1007/s10086-014-1432-0

Language：English   Publishing type：Research paper (scientific journal)  

Lignins are aromatic heteropolymers that arise from oxidative coupling of lignin precursors, including lignin monomers (p-coumaryl, coniferyl, and sinapyl alcohols), oligomers, and polymers. Whereas plant peroxidases have been shown to catalyze oxidative coupling of monolignols, the oxidation activity of well-studied plant peroxidases, such as horseradish peroxidase C (HRP-C) and AtPrx53, are quite low for sinapyl alcohol. This characteristic difference has led to controversy regarding the oxidation mechanism of sinapyl alcohol and lignin oligomers and polymers by plant peroxidases. The present study explored the oxidation activities of three plant peroxidases, AtPrx2, AtPrx25, and AtPrx71, which have been already shown to be involved in lignification in the Arabidopsis stem. Recombinant proteins of these peroxidases (rAtPrxs) were produced in Escherichia coli as inclusion bodies and successfully refolded to yield their active forms. rAtPrx2, rAtPrx25, and rAtPrx71 were found to oxidize two syringyl compounds (2,6-dimethoxyphenol and syringaldazine), which were employed here as model monolignol compounds, with higher specific activities than HRP-C and rAtPrx53. Interestingly, rAtPrx2 and rAtPrx71 oxidized syringyl compounds more efficiently than guaiacol. Moreover, assays with ferrocytochrome c as a substrate showed that AtPrx2, AtPrx25, and AtPrx71 possessed the ability to oxidize large molecules. This characteristic may originate in a protein radical. These results suggest that the plant peroxidases responsible for lignin polymerization are able to directly oxidize all lignin precursors.

DOI： DOI: 10.1371/journal.pone.0105332

Language：English   Publishing type：Research paper (scientific journal)  

Caryophyllene-6,7-epoxide and caryophyllene-6,7-episulfide can be easily synthesized
from β-caryophyllene by autoxidation or episulfidation. The bioactivities of β-caryophyllene
and its derivatives were investigated against the subterranean termite Reticulitermes speratus
Kolbe. The antifeedant, feeding, and termiticidal activities of each compound were tested using
no-choice, dual-choice, and non-contact methods. Antitermitic activities were not shown
by β-caryophyllene, but were observed for the oxide and sulfi de derivatives. Caryophyllene-
6,7-episulfi de showed especially high antifeedant and termiticidal activities. Thus, naturally
abundant, non-bioactive β-caryophyllene can be easily converted into an antitermite
reagent via a non-biological process.

Other Link： http://znaturforsch.com/c.htm

Language：English   Publishing type：Research paper (scientific journal)  

Putative Cationic Cell-Wall-Bound Peroxidase Homologues in Arabidopsis, AtPrx2, AtPrx25, and AtPrx71, Are Involved in Lignification.

DOI： 10.1021/jf400426g

Language：Japanese   Publishing type：Research paper (scientific journal)  

Language：English   Publishing type：Research paper (scientific journal)  

Tetramethylammonium hydroxide (TMAH) thermochemolysis of lignin: Formation of (E)-5-formyl-2,3,3´,4´-tetramethoxystilbene and its origins.

DOI： 10.1016/j.jaap.2010.08.008

Language：English   Publishing type：Research paper (scientific journal)  

Tetramethylammonium hydroxide (TMAH) thermochemolysis of 2-arylcoumaran lignin model compounds

Language：English   Publishing type：Research paper (scientific journal)  

Induced monoterpene and lignin production in mechanically stressed and fungal elicited cultured Cupressus lusitanica cells

Language：English   Publishing type：Research paper (scientific journal)  

Reaction mechanism of direct episulfidation of caryophyllene and humulene

Language：Japanese   Publishing type：Research paper (scientific journal)  

Language：English   Publishing type：Research paper (scientific journal)  

Language：English   Publishing type：Research paper (scientific journal)  

Language：English   Publishing type：Research paper (international conference proceedings)  

Combustion Synthesis of Composites Consisting of Ti-Si-C Ceramics from Sugi Bark and Alumina

Language：English   Publishing type：Research paper (international conference proceedings)  

Preparation of Polyurethane Film from Conifer Tree Bark Liquefied by PEG-Bisulfite Method

Language：English   Publishing type：Research paper (scientific journal)  

Signal transduction and metabolic flux of beta-thujaplicin and monoterpene biosynthesis in elicited Cupressus lusitanica cell cultures

Language：English   Publishing type：Research paper (international conference proceedings)  

Combustion Synthesis of Ti-Si-C System Composites using Woody Waste Material

Language：English   Publishing type：Research paper (scientific journal)  

Jasmonate and ethylene signalling and their interaction are integral parts of the elicitor signalling pathway leading to beta-thujaplicin biosynthesis in Cupressus lusitanica cell cultures

DOI： 10.1093/jxb/erh127

Language：English   Publishing type：Research paper (scientific journal)  

Involvement of cAMP signaling in elicitor-induced phytoalexin accumulation in Cupressus lusitanica cell cultures

DOI： 10.1046/j.1469-8137.2003.00976.x

Language：English   Publishing type：Research paper (scientific journal)  

Monoterpenes produced by Cupressus lusitanica cultured cells including a novel monoterpene (1S, 2S, 6S)-(+)-1,6-epoxy-4(8)-p-menthen-2-ol

DOI： 10.1080/1478641031000111525

Language：Japanese   Publishing type：Research paper (scientific journal)  

Effect of major inorganic nutrients on beta-thujaplicin production in a suspension culture of Cupressus lusitanica cells

DOI： 10.1007/s100860300027

Language：English   Publishing type：Research paper (scientific journal)  

Cell growth and nutrient uptake by cell suspensions of Cupressus lusitanica

DOI： 10.1007/s100860300001

Language：English   Publishing type：Research paper (scientific journal)  

Feedback regulation of beta-thujaplicin production and formation of its methyl ether in a suspension culture of Cupressus lusitanica

Language：English   Publishing type：Research paper (scientific journal)  

Production of beta-thujaplicin in Cupressus lusitanica suspension cultures fed with organic acids and monoterpenes

DOI： 10.1271/bbb.65.1027

Language：Japanese   Publishing type：Research paper (scientific journal)  

Cell growth and beta-thujaplicin production with a Cupressus lusitanica culture in jar fermentors

Language：English   Publishing type：Research paper (scientific journal)  

Improved beta-thujaplicin production in Cupressus lusitanica suspension cultures by fungal elicitor and methyl jasmonate

Language：English   Publishing type：Research paper (scientific journal)  

Biological activity of extracts from Cupressus lusitanica cell culture

DOI： 10.1007/BF01192336

Language：English   Publishing type：Research paper (scientific journal)  

BIOMECHANICAL PULPING USING WHITE-ROT FUNGUS IZU-154

Language：English   Publishing type：Research paper (scientific journal)  

BIOBLEACHING OF SOFTWOOD KRAFT PULP WITH WHITE-ROT FUNGUS IZU-154

Language：English   Publishing type：Research paper (scientific journal)  

BIOBLEACHING OF KRAFT PULP USING WHITE-ROT FUNGUS IZU-154

Responsible for pages：273-277 A factor controllig beta-thujaplicin production in suspension culture of Cupressus lusitanica   Language：English   Book type：Scholarly book

Presentation type：Oral presentation (general)  

Venue：京都   Country：Japan  

Cytochrome P450 enzyme oxidizing terpinolene in Cupressus lusitanica cultured cell (II)

Event date： 2024.3

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：京都大学   Country：Japan  

Event date： 2024.3

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：京都大学   Country：Japan  

ポプラ（Populus alba L.）由来の万能ペルオキシダーゼ、CWPO-C は成長旺盛な茎先端部や若い葉において強く発現する。また、シロイヌナズナの CWPO-C 過剰発現体はオーキシン欠乏性の表現型を示し、内生 IAA 量は野生型の約 1/10 に低下したこと、ならに in vitro 条件でリコンビナント CWPO-C はインドール-3-酢酸（IAA）を不活性な化合物へ代謝することが確認された[1]。これらのことから、CWPO-C はオーキシン不活性化を介して植物の成長を制御する可能性が示唆さ
れた。シロイヌナズナ AtPrx71 は CWPO-C とアミノ酸配列の相同性が高く、リコンビナント AtPrx71の基質反応特性は CWPO-C と非常に類似していることから、AtPrx71 は CWPO-C のオーソログだと考えられる。従って、AtPrx71 も CWPO-C と同様にオーキシンの不活性化を介し、分化や成長に対して抑制的に働いている可能性が予想された。そこで本研究では、AtPrx71 に注目し、シロイヌナズナ野生型（以降 WT）、AtPrx71 欠損変異体(以降、KO)と AtPrx71 過剰発現体(以降、OE)について、主茎の成長や重力応答屈性についてCWPO-C 過剰発現体との比較を行うとともに、主茎基部切片染色と GUS 染色を行い、AtPrx71 ならびに CWPO-C の植物成長における機能を明らかにすることを目的とした。

Event date： 2024.3

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：京都大学   Country：Japan  

Event date： 2023.9

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：別府市APU   Country：Japan  

モデル植物であるシロイヌナズナのAtPrx71 に注目し、シロイヌナズナ野生型（以降WT）、AtPrx71 欠損変異体(以降、KO)とAtPrx71 過剰発現体(以降、OE)について、主茎の成長や重力応答による屈性試験を行い、CWPO-C 過剰発現体との比較を行うことで、AtPrx71 ならびにCWPO-C の植物成長における機能を明らかにすることを目的とした。

Event date： 2023.4

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：福岡   Country：Japan  

Polymerization and aggregation morphology of syringyl monolignols during the formation of artificial cell wall using rCWPO-C

Event date： 2023.4

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：福岡   Country：Japan  

The cationic cell-wall-bound peroxidase (CWPO-C) involved in plant growth and lignification in Poplar

Event date： 2023.3 - 2023.4

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：福岡   Country：Japan  

Attempt to establish analysis of some sesquiterpenes contained in cedar using GC-MS for JAS standards

Event date： 2023.3 - 2023.4

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：福岡   Country：Japan  

Effect on the plant growth and its regulatory manner by the knockout of <em>CWPO-C</em> orthologous,<em> AtPrx2, AtPrx25, AtPrx71</em> in Arabidopsis thaliana

Event date： 2023.3

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：福岡   Country：Japan  

Effect of different amounts of cedar lumber on psychological and physiological responses

Event date： 2022.11

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：熊本（リモート）   Country：Japan  

スギ材(Cryptomeria japonica)を内装材として用いた場合の木材量を変化させることで揮発性成分量を変化させ、木材量がヒトの心理・生理反応に及ぼす影響を検討した。

Event date： 2022.11

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：熊本（リモート）   Country：Japan  

Our results demonstrated that the overexpression of CWPO-C, decreased the IAA content in Arabidopsis and resulted in the phenotype that is consistent with the typical traits of the auxin deficiency. This strongly suggests that CWPO-C plays a role in the regulation of plant growth through the catabolizing IAA.

Event date： 2022.11

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：熊本（リモート）   Country：Japan  

JAS制度を活用し、スギ材の更なる需要を高めるために、JAS化を目指したスギに含有される機能性成分であるテルペン類の分析法について報告する。

Event date： 2022.3

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：リモート   Country：Japan  

Potential utilization of bamboo alkali lignin as an antioxidant and α-glucosidase inhibitory activator

Event date： 2022.3

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：リモート   Country：Japan  

The chemical response of Cupressus lusitanica seedling against chemical and physical stimulations

Event date： 2022.3

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：リモート   Country：Japan  

Plant growth regulation by CWPO-C through the IAA metabolism

Event date： 2022.3

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：リモート   Country：Japan  

Development of syringyl lignin-cellulose conjugate film using ferulic acid scaffold mediated by rCWPO-C

Event date： 2020.11 - 2020.3

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：リモート   Country：Japan  

Event date： 2018.3

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：京都府立大学（京都）   Country：Japan  

新たにアルカリ蒸解法で得られたタケリグニン(アルカリタケリグニン)を原料として、 CA を添加した CF の調製を行い、PEG・バイサルファイト法で調製した CF と比較した。

Event date： 2017.10

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：名古屋大学（名古屋）   Country：Japan  

Preparation of carbon fiber by electrospinning using　liquefied bamboo from PEG-bisulfite method

Event date： 2017.9

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：福岡大学（福岡）   Country：Japan  

PEG・バイサルファイト法で得られた液化物に酢酸セルロースもしくは三酢酸セルロースを添加してエレクトロスピニングおよび炭化処理を行い、カーボンファイバーの調製を試みた。

Event date： 2017.6

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：東京大学（東京）   Country：Japan  

Carbon material from liquefied bamboo-lignin by PEG-bisulfite method

Event date： 2017.3

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：福岡市   Country：Japan  

植物は揮発性有機化合物（VOC）を放出し，シグナルとして利用する。我々はメキシコイトスギ培養細胞が4種のモノテルペンを気相に放出，感知し，連鎖的なシグナル伝達を行うことを明らかにしているが，このような連鎖的シグナリングについて他樹種での知見はほとんど得られていない。モノテルペンによるシグナル伝達の普遍性や樹種間コミュニケーションの可能性についての議論を目標とし，本研究では，スギ，ヒノキの培養細胞のシグナル伝達経路について調べた。今回，ヒノキおよびスギ培養細胞が外因性のモノテルペン暴露に対して示した反応について報告する。実験の結果，メキシコイトスギ培養細胞のシグナル伝達経路は，ヒノキ培養細胞の経路と大部分が共通しており，スギ培養細胞の経路とは一部のみが共通であることが明らかになった。

Event date： 2017.3

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：福岡市   Country：Japan  

近年，モノリグノールの合成・輸送・重合というリグニン生合成機構の一端は明らかにされつつあるものの，リグニンの不均一性を制御する機構についてはほとんど知見が得られていない。そこで，本研究ではLasermicrodissection（LMD）により採取した試料の，熱分解GC-MS（Py-GC-MS）による組織・細胞種ごとの詳細なリグニン分析を試みている。今回，リグニン単位間5-5 結合型モデル化合物の二量体熱分解生成物の確認を行い，LMD によるダイセクションサンプル中での5-5 型の探索を行った。その結果，リグニン末端に形成される5-5 型結合の指標となる熱分解物が確認され，それが微量なダイセクションサンプル中でも検出可能であることが示された。また，多年生ポプラ個体の木部を数年輪にかけて解析し，リグニン単位間結合変化の調査も行った。

Event date： 2017.3

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：福岡市   Country：Japan  

近年，国内タケの新規用途開発が求められているが，未だにタケの主要成分であるリグニンの画期的な利用手法は報告されていない。本研究室では，PEG・バイサルファイト法をタケに適用することにより，タケ/PEG・バイサルファイト液化物の調製を行った結果，処理温度200℃においてリグニンのみを選択的に液化可能にすることを見出した。本発表では，タケ液化物の新規利用法として，エレクトロスピニング法および炭化処理を液化物に適用することによりカーボン繊維の調製を試みたところ，タケ液化物および三酢酸セルロース混合試料では，繊維幅約1. 0 μm から500 nm を有する炭素繊維集合体が走査電子顕微鏡により観察されたことから，タケ液化物からカーボンナノファイバー（CNF）不織布を調製可能であることが示唆された。本発表では，CNF 不織布を調製するエレクトロスピニング条件の検討結果および顕微レーザーラマン分光による各試料の構造評価について報告する。

Event date： 2017.3

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：福岡市   Country：Japan  

近年，竹林拡大による森林荒廃が重要な問題として指摘されており，竹の活用方法が求められている。その中でも，タケの主要成分であるリグニンは複雑な三次元構造を有する高分子のため，未だに高付加価値化する画期的な手法が報告されていない。本研究では，PEG・バイサルファイト法がタケ液化に有効であることを検討した。その結果，処理温度200℃においてリグニンを選択的に液化可能にすることを見出し，処理温度250℃において多糖画分を含めて高い可溶化を示した。また，両方の処理温度においてエレクトロスピニング法および炭化処理を液化物に適用することによりカーボンナノファイバーの調製を試みた。各液化物由来の炭化試料に対するXRD 測定法による結晶性の評価結果，ならびに，各炭化試料の形態観察結果についても報告する。

Event date： 2017.3

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：福岡市   Country：Japan  

タケリグニンの新たな利用法の開発にむけて，これまでの研究では脱脂タケから亜リン酸水熱処理とソーダアントラキノン処理によりリグニンを分離した。しかし，得られたリグニンの構造解析の結果，非縮合型構造が減少しており，水素化によるリグニン由来低分子化合物の収率が低かった。そこで，今回は直接ソーダAQ 処理によりリグニンを単離した。処理後の各試料の化学組成分析を行い，単離条件の最適化を検索し，また単離条件に伴うリグニンの化学構造変化も解析した。

Event date： 2017.3

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：福岡市   Country：Japan  

スギの無垢材を内装に用いた建物と表面が塗装やビニールクロスで覆われた内装材を用いた建物の室内において，年間を通して揮発性成分を定期的に捕集し，ガスクロマトグラフ質量分析計（GC/MS）により分析を行い，比較した。分析したところ，どちらの実験棟においても，気温が低い冬季と比較して，気温の高い夏季でセスキテルペン類の濃度が高くなる傾向が見られた。

Event date： 2016.10

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：京都   Country：Japan  

国産タケの有効利用を目指した新規バイオリファイナリー開発が求められているが、タケの主要成分であるリグニンの画期的な手法は報告されていない。そこで、本研究では、PEG・バイサルファイト法をタケ粉に適用することにより、タケ/PEG・バイサルファイト液化物を調製した。その結果、処理温度250 ℃において多糖画分を含めて高い可溶化を示し、一方、処理温度200 ℃においてリグニンのみを選択的に液化可能にすることを見出した。また、各処理条件で得られた液化物からポリウレタンフォームを調製し、各ポリウレタンフォームを固体粘弾性測定に供したところ、リグニン含量の多いポリウレタンフォームはコントロールと比較して高い貯蔵弾性率を示し、多糖画分が多いリグニン含有ポリウレタンフォームは高温で急な貯蔵弾性率の低下を示した。本発表では、タケのPEG・バイサルファイト法の最適化条件、各ポリウレタンフォームの固体粘弾性測定による特性評価について報告する。

Event date： 2016.10

Language：Japanese   Presentation type：Oral presentation (general)  

Country：Japan  

Lignification is known to occur in xylem tissue before programmed cell death (PCD). Recently,
it is suggested that lignification still proceeds after PCD in some reports, and our previous study also indicated
the similar results; the yields of pyrolysis products and S/G ratio, which derived from β-O-4 linked lignin
determined by laser micro dissection (LMD) and pyrolysis-GC-MS (Py-GC-MS), increased and decreased,
respectively, along with the radial direction within a growth ring in which the xylem fiber cells were already
suffered from PCD. In order to understand more detailed lignification process, in this study we analyzed β-β,
β-1, β-5 internal linkage in lignin using LMD and Py-GC-MS. First, we examined detection limit of Py-GC-MS
for these dimeric pyrolysis products from the dissected samples prepared by LMD. The minimum requirement
of the dissected area was 0.3 mm2 in. Then, the abundances of β-β, β-1, β-5 subunits in wood meal prepared
from old poplar stem, lignified fiber cells and unlignified fiber cells in a year-old P. alba stem were compared.
β-1 subunit was found in a young stem but not in wood meal, which indicate that β-1 is formed at an early stage
in differentiation of xylem fiber cells

Event date： 2016.9

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：鹿児島   Country：Japan  

タケのバイオリファイナリー技術の開発を目指し、タケリグニンの選択的分離と分離成分の高付加価値物質への変換を検討することにした。これまでの研究ではまず既報を元に、亜リン酸水熱処理によってヘミセルロースを除去し、得られた残渣からソーダアントラキノン処理によってリグニンを分離することにした。しかし、得られたリグニンの構造解析の結果、リグニンの縮合反応が生じており、低分子の生成物の収率が極めて低いという結果が得られた。そのため、水熱処理を行わず、直接アルカリによる処理を行うことによる高付加価値利用の可能性についても目指すことにした。

Event date： 2016.9

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：鹿児島   Country：Japan  

Liquefaction of bamboo by PEG-bisulfite method

Event date： 2016.9

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：鹿児島   Country：Japan  

Liquefaction of bamboo by PEG-bisulfite method

Event date： 2016.6

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：東京   Country：Japan  

Characterizations of polyurethane foam prepared from liquefied bamboo and liquefaction of bamboo by PEG-bisulfite method

Event date： 2016.6

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：東京   Country：Japan  

Development of bamboo biorefinery
Production of phenolic monomers from bamboo lignin

Event date： 2016.6

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：東京   Country：Japan  

Metabolic Engineering of Basidiomycete for the Utilization of Hemicellulose of Bamboo

Event date： 2016.3

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：名古屋大学   Country：Japan  

植物は外部からのストレスに反応して種々の揮発成分を放出することが明らかになっている。
その多くは自分の身を守るために役立つものである。またそれ以外に別の器官や植物へのシグナ ルとして働く成分もあることが知られている 1。
メキシコイトスギ培養細胞は、エリシターストレスに反応して 10 種類のモノテルペンを放出す る。そのうち 4 つのモノテルペンはサビネン→γ-テルピネン→p-シメン→テルピノレンという段階 的なストレスシグナル伝達経路を作っている。このシグナル伝達の結果、抗菌活性を示す心材成 分であるヒノキチオールの生産が促されることが示されている 2。
しかし、このようなモノテルペンによる段階的なシグナル伝達はメキシコイトスギの、特に培 養細胞でしか確認されておらず、その他の植物種については十分な知見がほとんど得られていな い。一方で、ヒノキから放出されるモノテルペンはメキシコイトスギ培養細胞から放出されるも のに似ていることが明らかになっている 3。そこで本研究では、ヒノキ培養細胞の気相シグナル伝 達を調べ、メキシコイトスギ培養細胞のシグナル伝達との比較を行った。

Event date： 2016.3

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：名古屋大学   Country：Japan  

竹は古くから日用製品および文学作品として、日本人の生活および文化に根差した素材である。
しかしながら、近年、拡大竹林とそれに伴う荒廃森林が問題になっている。そのため、竹林の整 備や拡大・侵入防止策および竹の新規用途開発が求められている。最近では、竹の主要成分であ るセルロースからセルロースナノファイバーを調製する手法が報告されている 1, 2)。一方、リグニ ンは複雑な高次構造の高分子のため、未だに高付加価値化する有効な手法が報告されていない。
坂井らはグリコール・バイサルファイト法をスギ樹皮に適用し、リグニンを含むスギ樹皮全体 の高い液化率を得た 3)。そこで本発表では、このグリコールとして、ポリエチレングリコール(PEG) を用いた PEG・バイサルファイト法をタケ粉に適用することにより、タケ/PEG・バイサルファイ ト液化物の調製を試みた。さらに、調製した液化物から概報 4)を参考に硬質ポリウレタンフォー ムの調製も試みた。
【実験方法】
脱脂処理した竹粉末 4 g に、PEG-400 を 12 mL および 6 wt%-亜硫酸水素ナトリウム水溶液 10 mL
を添加し、十分に撹拌を行った。その後、オートクレーブに封入し、オーブンにて所定処理温度 および保持時間で加熱処理を行った。処理後、氷水で急冷し、反応停止した試料をガラス繊維ろ 紙を用いて吸引ろ過を行った。ろ過後、残渣をジオキサンで洗浄および凍結乾燥処理を行った。 乾燥後の残渣の重量を計測し、タケ粉末からの可溶化率を算出した。また、残渣は酸不溶性リグ ニン (AIL) および酸可溶性リグニン (ASL) 測定に供した。

【結果および考察】
Table は処理温度 250 °Cにおける各処理時間のタケ/PEG・バイサルファイト液化物の可溶化率、
AIL および ASL 保持率を示している。60 min 以上の処理時間の場合、30 min の条件よりも大幅に 高い可溶化率を示した。また、60 および 90 min の処理時間では各保持率に大きな差異が見られな かった。以上の結果から、処理温度 250 °Cにおいて、試料の液化条件に適した処理時間は 60 min である可能性が示唆された。一方、リグニンのみを選択的に液化可能な処理温度 200 °Cにおいて 得られた試料の保持率、各成分の組成およびポリウレタンフォームの物性分析結果については本 発表にて報告する。
【謝辞】 本研究は農林水産業・食品産業科学技術研究推進事業(シーズ創出ステージ:27006A、バン
ブーリファイナリー技術開発による竹林有効利用の先進的九州モデル構築)の助成によって行わ れた。
【参考文献】
1) Tsuboi, K.; Yokota, S.; Kondo, T. Nordic Pulp & Paper Research Journal 2014, 29, (1), 69-76.
2) 清水喜作,; 田中裕之,; 橋場洋美. 特許公報(B2) 2015, 5690303, 1-8.
3) Towatari (Ueno), T.; Ashitani, T.; Sakai, K. Journal of Wood Science 2002, 48, (4), 348-351. 4) 戸渡智子ら. 木材工業 2007, 62, (8), 358-363.

Event date： 2015.10

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：福岡県大分市   Country：Japan  

培養細胞を用いた実験ではC. lusitanicaはテルペンのシーケンスで防御信号を伝達していると考えられる。しかし、この実験は未分化の培養細胞を用いた実験であり、実際の樹木が同じシグナル伝達経路を持つかどうかは未だ明らかになっていない。そこで、本研究では植物個体においてモノテルペンの暴露実験を行い、同様のシグナル伝達が個体にも存在するかどうかを検討した。

Event date： 2015.10

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：福岡県大分市   Country：Japan  

これまでのファイトレメディエーションに関する研究では、単一重金属汚染に関する研究が行われてきたが、複合重金属汚染の浄化に関する研究はほとんど行われていない。そこで本研究では、日本で広く存在している植物であり、土壌中カドミウムの浄化に効果が報告されているハクサンハタザオ(Arabidopsis halleri ssp.gemmifera) に注目し、カドミウムと鉛の複合汚染時の重金属吸収能や重金属耐性の機構に関して検討を行った。

Event date： 2015.3

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：タワーホール船堀 (東京都江戸川区船堀４丁目１番１号)   Country：Japan  

Event date： 2015.3

Language：English   Presentation type：Oral presentation (general)  

Venue：TowerHall Funabori, Tokyo   Country：Japan  

Lignin is the phenylpropanoid polymer composed of three monolignols through the several linkages generated by the radical couplings. The biosynthesis of monolignols has ever been studied using mainly homogenized organs or tissues which contains several types of cells. However, many reports indicated that deposition of lignin is heterogeneous, and lignin biosynthesis may be highly regulated depending on cell types or tissues. Further, it was reported that some genes related in monolignol biosynthesis were expressed in ray parenchyma cells in the lignified area1), and CWPO-C that is capable of lignin polymerization located in the cell corners of the matured xylem2). These reports suggest that lignification still keeps proceeding in the fiber cells after their programmed cell death. Recently, in cell culture systems, lignification of tracheary element cell walls has been indicated to continue to increase after the cell death3). In order to understand more detailed lignification process, we have tried to obtain the cell-type- or tissues- specific information on lignin biosynthesis mechanism.
In this study we employed the laser micro dissection (LMD) technique to dissect a current year growth ring of the Populus alba secondary xylem that contains approximately 70 cells in a line (Fig.) into several sections, at least 8 stage of different lignified levels. The dissected sections were subjected to Py-GC-MS, and the pyrolysis products and S/G ratio derived from β-O-4 linked lignin in the dissected samples were determined. The increase of pyrolysis products and decrease of S/G ratio along with the radial direction from cambial to pith within a growth ring suggested that lignification continues in the mature xylem fiber cells in which lignification has generally been thought to be completed. On the other hand acetocarmine staining of nuclei and fragments of DNA was observed only in the cells within the area of 20th or 30th from cambium (Fig.), but not in the older cells than them, suggesting that the fiber cells in the older cells area than 20th or 30th cells were already suffered from programmed cell death. These results imply that lignification in the fiber cells still continues even after programmed cell death, probably with the assistance of ray parenchyma cells.

Event date： 2014.3

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：松山市   Country：Japan  

Event date： 2014.3

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：松山市   Country：Japan  

We found very quick de novo production of terpenes by terpensynthases and its time course information revealed signaling mechanism.

Other Link： de novo Production of terpenes by terpensynthases and its function as signaling

Event date： 2013.9

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：福岡県福岡市   Country：Japan  

Event date： 2013.9

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：福岡県福岡市   Country：Japan  

Time course change of olefin monoterpenes involved in sigmnal transfar on Cupressus lusitanica cultured cell.

Event date： 2013.9

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：福岡県福岡市   Country：Japan  

Event date： 2013.6

Language：English   Presentation type：Oral presentation (general)  

Venue：Kolymvari   Country：Greece  

Air born defense signal transduction cascade with monoterpenes on Cupressus lusitanica culture cells

Other Link： http://terpnet2013.pharm.auth.gr

Event date： 2013.6

Language：English   Presentation type：Oral presentation (general)  

Venue：Kolymvari   Country：Greece  

Terpinolene is the first olefin monoterpene intermediate to a tropolone, β-thujaplicin –Potential novel pathway to tropolone ring–

Other Link： http://terpnet2013.pharm.auth.gr

Event date： 2013.3

Language：Japanese  

Venue：盛岡市   Country：Japan  

Terpinolene is a intermediate of beta-thujhaplicin biosynthesis

Event date： 2013.3

Venue：盛岡市   Country：Japan  

リグニンは3種のフェニルプロパノイドの合成、細胞壁への輸送、高分子化という過程により植物細胞壁に蓄積される。一方で、リグニンの不均一性を制御する機構についてはほとんど知見が得られていない。そこで、木化過程を詳細に解析する技術が不可欠である。本研究では、Laser microdissection（LMD）により採取した試料の、熱分解GC-MS（Py-GC-MS）によるリグニン分析を行った。分析可能な最小サンプル量の検討を行った結果、放射方向に1~3細胞列（約0.7 μg）程度の試料で、Py-GC-MSによるリグニン由来生成物の定性・定量が可能であった。さらに放射方向への段階的なリグニン分析の結果、細胞壁形成初期ではS型がG型よりも合成され、リグニンの蓄積とともにG型の含有率の増加が確認された。これらの結果からLMDのリグニン分析への有用性が証明され、リグニン生合成の時間的変化情報が取得できた。

Event date： 2013.3

Venue：盛岡市   Country：Japan  

Signal transduction with olefin monoterpene in Cuplessus lusitanica cultured cell

Event date： 2012.12

Presentation type：Oral presentation (general)  

Venue：宮崎県木材利用技術センター   Country：Japan  

Event date： 2012.7 - 2011.7

Presentation type：Oral presentation (general)  

Venue：Vilnius   Country：Other  

The relationship between the time courses of the β-thujaplicin production and of the activities of terpinolene oxygenases in Cupressus lusitanica cultured cells

Other Link： https://sites.google.com/a/chemecol.org/welcome/meeting2012

Event date： 2012.3

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：札幌市   Country：Japan  

Other Link： http://www.jwrs.org/index-e.html

Event date： 2012.3

Presentation type：Oral presentation (general)  

Venue：札幌市   Country：Japan  

Other Link： http://www.jwrs.org/index-e.html

Event date： 2011.9

Presentation type：Oral presentation (general)  

Venue：Miyazaki, Japan   Country：Japan  

Monoterpene biosynthesis -General idea and a case of terpinolene metabolism in Cupressus lusitanica-

Event date： 2011.9

Presentation type：Oral presentation (general)  

Venue：山形大学農学部、鶴岡市   Country：Japan  

Lignin formation on Cupressus lusitanica cultured cell at phytoalexine producing condition.

Event date： 2011.5

Presentation type：Oral presentation (general)  

Venue：Kalmar   Country：Sweden  

Bioactivities of epoxide and episulfide derived from medium ring sesquiterpene hydrocarbons

Other Link： http://lnu.se/about-lnu/conferences/terpnet-2011?l=en

Event date： 2011.5

Presentation type：Oral presentation (general)  

Venue：Kalmar   Country：Sweden  

Oxidative metabolism of monoterpene in Cupressus lusitanica culture cells

Other Link： http://lnu.se/about-lnu/conferences/terpnet-2011?l=en

Event date： 2010.10

Presentation type：Oral presentation (general)  

Venue：甲府   Country：Japan  

Terpinoene metabolism in Cupressus lusitanica cultured cell

Event date： 2010.7 - 2011.8

Presentation type：Oral presentation (general)  

Venue：Tours   Country：France  

Longifolene as a precursor of plant self‐defensive compounds

Other Link： http://www.atout-org.com/isce2010/topics

Event date： 2010.7 - 2011.8

Presentation type：Oral presentation (general)  

Venue：Tours   Country：France  

The signaling effect of volatile monoterpenes emitted from elicited Cupressus lusitanica cultured cells

Other Link： http://www.atout-org.com/isce2010/topics

Event date： 2010.7 - 2011.8

Presentation type：Oral presentation (general)  

Venue：Tours   Country：France  

Metabolism of terpinolene which is produced by fungal attack in Cupressus lusitanica culture cells

Other Link： http://www.atout-org.com/isce2010/topics

Event date： 2010.3 - 2011.3

Presentation type：Oral presentation (general)  

Venue：京都市   Country：Japan  

Facilitation of phytoalexine production by gaseous monoterpene in Cupressus lusitanica cell culture

Other Link： http://www.jwrs.org/index-e.html

Event date： 2010.3 - 2011.3

Presentation type：Oral presentation (general)  

Venue：京都市   Country：Japan  

Anti-ant activity of Humulene derivatives

Other Link： http://www.jwrs.org/index-e.html

Event date： 2010.3 - 2011.3

Presentation type：Oral presentation (general)  

Venue：京都市   Country：Japan  

Oxidative metabolism of monoterpenes in Cupressus lusitanica.

Other Link： http://www.jwrs.org/index-e.html

Event date： 2010.3

Presentation type：Oral presentation (general)  

Venue：宮崎市   Country：Japan  

Pyrolysis of kenaf at lower temprature (V)

Event date： 2010.3

Venue：宮崎市   Country：Japan  

Event date： 2010.3

Presentation type：Oral presentation (general)  

Venue：宮崎市   Country：Japan  

Stereo-selective oxidation of monoterpene in Cupressus lusitanica cells (3)

Event date： 2010.3

Presentation type：Oral presentation (general)  

Venue：宮崎市   Country：Japan  

Signaling effect of monoterpenes emitted from Cupressus lusitanica

Event date： 2009.11

Presentation type：Oral presentation (general)  

Venue：那覇市   Country：Japan  

Pyrolysis of kenaf at lower temprature (IV)

Event date： 2009.11

Presentation type：Oral presentation (general)  

Venue：那覇市   Country：Japan  

Stereo-selective oxidation of monoterpene in Cupressus lusitanica cells (2)

Event date： 2009.3

Presentation type：Oral presentation (general)  

Venue：松本市   Country：Japan  

Antifungal activity of emitted monoterpenes from stressed Cupressus lusitanica cells

Other Link： http://www.jwrs.org/wood2009/

Event date： 2009.3

Presentation type：Oral presentation (general)  

Venue：松本市   Country：Japan  

Pyrolysis of kenaf at lower temprature　(3)

Other Link： http://www.jwrs.org/wood2009/

Event date： 2009.3

Presentation type：Oral presentation (general)  

Venue：松本市   Country：Japan  

Other Link： http://www.jwrs.org/wood2009/

Event date： 2008.12

Presentation type：Oral presentation (general)  

Venue：静岡県静岡市   Country：Japan  

Event date： 2008.8

Presentation type：Oral presentation (general)  

Venue：大分県日田市   Country：Japan  

Event date： 2008.3

Presentation type：Oral presentation (general)  

Venue：つくば   Country：Japan  

Induced isoprenoid biosynthesis in response to mechanical stress in cultured Cupressus lusitanica cells

Other Link： http://www.jwrs.org/wood2008/

Event date： 2008.3

Presentation type：Oral presentation (general)  

Venue：つくば   Country：Japan  

Other Link： http://www.jwrs.org/wood2008/

Event date： 2007.11

Language：Japanese   Presentation type：Oral presentation (general)  

Country：Japan  

Phenolic 2-arylcoumarans 1-6 were subjected to tetramethylammonium hydroxide (TMAH)
thermochemolysis (500 ºC/4 s) to investigate reactions occurring in lignin specifically under these conditions.
Products were monitored by gas chromatography/mass spectrometry (GC/MS). GC/MS signals corresponding
to dimers overwhelmed all GC/MS traces. (2-Phenyl-2,3-don-3-yl) methan-1-ols (1 and 6) predominantly gave
2,3,3',4'-tetramethoxystilbenes involving in a loss of the γ-CH2OH substituent.
3-Methyl-2-phenyl-2,3-dihydrobenzofurans (2-5) gave a mixture of
1-(3,4-dimethoxyphenyl)-2-(2,3-dimethoxyphenyl)-1-methoxypropans and 2,3,3’,4’- tetramethoxy-stilbenes
having the γ-methyl group. Side chains –CH=CHCH2OH and -COOH on ring B were simply methylated to
produce the corresponding methyl ether and ester, respectively. A –CH=CHCH3 Side chain was preserved intact,
whereas a –CH=CHCHO side chain changed to a -CHO side chain. This was confirmed by isolating
5-formyl-2,3,3',4'-tetramethoxystilbene as an off-line TMAH thermo- chemolysis product from
3-[2-(4-hydroxy-3-methoxyphenyl)-3- hydroxymethyl-7-methoxy-2, 3- dihydrobenzofuran-5-yl] propenal.

Event date： 1990.10

Presentation type：Oral presentation (general)  

Venue：東京   Country：Japan  

Event date： 1990.4

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：つくば   Country：Japan  

微生物（IZU−154株）でクラフトパルプを処理することにより、残留リグニンが減少し漂白効果が得られた。

Presentation type：Oral presentation (general)  

Venue：熊本   Country：Japan  

The production of beta-thujaplicin in a suspension culture of Cupressus lusitanica

Presentation type：Oral presentation (general)  

Venue：高知   Country：Japan  

Presentation type：Oral presentation (general)  

Venue：高知   Country：Japan  

Presentation type：Oral presentation (general)  

Venue：静岡   Country：Japan  

Presentation type：Oral presentation (general)  

Venue：静岡   Country：Japan  

Presentation type：Oral presentation (general)  

Venue：Jerusalem   Country：Israel  

Presentation type：Oral presentation (general)  

Venue：東京   Country：Japan  

Presentation type：Oral presentation (general)  

Venue：Tsukuba   Country：Japan  

Presentation type：Oral presentation (general)  

Venue：東京   Country：Japan  

Presentation type：Oral presentation (general)  

Venue：京都   Country：Japan  

Presentation type：Oral presentation (general)  

Venue：京都   Country：Japan  

Presentation type：Oral presentation (general)  

Venue：Tsukuba   Country：Japan  

Presentation type：Oral presentation (general)  

Venue：Tsukuba   Country：Japan  

Presentation type：Oral presentation (general)  

Venue：東京   Country：Japan  

Presentation type：Oral presentation (general)  

Venue：福岡   Country：Japan  

Presentation type：Oral presentation (general)  

Venue：岐阜   Country：Japan  

Presentation type：Oral presentation (general)  

Venue：Lexington, KY   Country：United States  

Presentation type：Oral presentation (general)  

Venue：札幌   Country：Japan  

mRNAs induced by elicitation in Cupressus lusitanica cultured cells.

Presentation type：Oral presentation (general)  

Venue：Wageningen   Country：Netherlands  

Presentation type：Oral presentation (general)  

Venue：大阪   Country：Japan  

Presentation type：Oral presentation (general)  

Venue：名古屋   Country：Japan  

Factors at DHP preparation

Presentation type：Oral presentation (general)  

Venue：Nagoya   Country：Japan  

Presentation type：Oral presentation (general)  

Venue：秋田   Country：Japan  

SPME analysis of the volatile terpenoids produced from Cupressus lusitanica cultured cell.

Presentation type：Oral presentation (general)  

Venue：秋田   Country：Japan  

Presentation type：Oral presentation (general)  

Venue：新潟   Country：Japan  

Presentation type：Oral presentation (general)  

Venue：札幌   Country：Japan  

Pyrolysis of Lignin: Products Stemming from beta-O-4 subunits

Presentation type：Oral presentation (general)  

Venue：能代   Country：Japan  

Presentation type：Oral presentation (general)  

Venue：Asan   Country：Korea, Republic of  

Presentation type：Oral presentation (general)  

Venue：広島   Country：Japan  

Cloning of Cupressus lusitanica terpene synthese cDNA IV

Other Link： http://www.jwrs.org/wood2007/data/index-j.html

Presentation type：Oral presentation (general)  

Venue：広島   Country：Japan  

Presentation type：Oral presentation (general)  

Venue：久留米   Country：Japan  

Monoterpene emission from Cupressus lusitanica cell culture with fungal elicitor

Language：Japanese   Publishing type：Article, review, commentary, editorial, etc. (scientific journal)  

Development of the integrated bio-refinery technology for bamboo utilization

Language：Japanese   Publishing type：Article, review, commentary, editorial, etc. (scientific journal)  

Language：Japanese   Publishing type：Article, review, commentary, editorial, etc. (scientific journal)  

Understanding chemical language between plants with a model of cultured woody plant

Language：Japanese  

Language：Japanese   Publishing type：Internal/External technical report, pre-print, etc.  

Language：Japanese   Publishing type：Internal/External technical report, pre-print, etc.  

Language：Japanese   Publishing type：Internal/External technical report, pre-print, etc.  

Type：Peer review 

Number of peer-reviewed articles in foreign language journals：3

Number of peer-reviewed articles in Japanese journals：1

Proceedings of International Conference Number of peer-reviewed papers：0

Proceedings of domestic conference Number of peer-reviewed papers：0

Type：Peer review 

Number of peer-reviewed articles in foreign language journals：2

Type：Competition, symposium, etc. 

Number of participants：100

Type：Peer review 

Number of peer-reviewed articles in foreign language journals：1

Type：Competition, symposium, etc. 

Number of participants：1,500

Type：Competition, symposium, etc. 

Type：Competition, symposium, etc. 

Number of participants：1,000

Type：Competition, symposium, etc. 

Type：Competition, symposium, etc. 

Type：Competition, symposium, etc. 

Type：Competition, symposium, etc. 

Type：Competition, symposium, etc. 

Number of participants：1,000

Type：Competition, symposium, etc. 

Type：Competition, symposium, etc. 

Type：Competition, symposium, etc. 

Number of participants：1,500

Type：Competition, symposium, etc. 

Type：Competition, symposium, etc. 

Type：Competition, symposium, etc. 

Number of participants：200

Type：Competition, symposium, etc. 

Type：Competition, symposium, etc. 

Number of participants：150

Type：Competition, symposium, etc. 

Type：Competition, symposium, etc. 

Type：Competition, symposium, etc. 

Number of participants：80

Type：Competition, symposium, etc. 

Number of participants：1,600

Type：Competition, symposium, etc. 

Type：Competition, symposium, etc. 

Type：Competition, symposium, etc.