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写真a

ナカヤマ ジロウ
中山 二郎
NAKAYAMA JIRO
所属
農学研究院 生命機能科学部門 教授
農学部 生物資源環境学科(併任)
生物資源環境科学府 生命機能科学専攻(併任)
職名
教授
プロフィール
研究活動 1.アジア人の食と腸内フローラと健康の関連性に関する調査研究  アジアの諸外国と共同研究で、多様な食文化を有するアジア人の食と腸内フローラと健康について調査し、その関連性を見出すための研究を進めている。特に、当研究室では、次世代DNAシーケンサーや質量分析計、NMR等を用いるオミックス解析手法に加えて、培養法を基盤とした微生物学的研究も展開し、トップダウンとボトムアップの両者から、複雑な腸内フローラの機能の理解に努めている。 2.ヒト腸内細菌のケミカルコミュニケーション  ヒト腸内細菌が細胞間コミュニケーションの手段として用いている、ペプチドフェロモンをシグナルとするシグナル伝達系の実体について遺伝子レベルからアプローチする。最終的には腸内細菌のエコシステムを制御するペプチドを見出し、腸内細菌叢の改善を人為的にコントロールすることを目指している。 3.グラム陽性細菌のクオラムセンシングを標的とした抗感染症剤の開発 腸球菌、ブドウ球菌、ウェルシュ菌、ディフィシル菌などの日和見感染菌は環状ペプチドをオートインデューサーとして同種菌の細胞密度を感知し病原性の発現を制御するクオラムセンシング機構を有している。我々は、それらのクオラムセンシングの分子機構を解明し、さらにはそれを標的とした抗感染症剤の開発を行っている。 4.腸内細菌叢データを利用した乳幼児におけるアレルギー発症の早期診断法の確立  ヒト乳幼児の腸内細菌叢とアレルギー発症の関連性を実験疫学的に調査し、アレルギー発症の早期診断マーカーを見出すことを目指している。(現在行っていない) 5.発酵食品の細菌叢の解析  日本を代表する伝統的スローフードであるぬか床の細菌叢、およびモンゴル人の健康を支える発酵馬乳について、細菌叢とその複合微生物系における代謝に関する研究を行っている。 6.乳酸菌の生産するバクテリオシンに関する研究  自然界に多く存在する乳酸菌の生産するバクテリオシンの構造や、その生合成機構や発現調節機構等を解析している。 7.腸球菌のプラスミド接合伝達を制御する性フェロモンに関する研究(現在本研究は行っていない)
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研究分野

  • ライフサイエンス / 生物有機化学

  • ライフサイエンス / 応用生物化学

  • ライフサイエンス / 応用微生物学

学位

  • 博士(農学)

経歴

  • 九州大学大学院農学研究院 生命機能科学部門システム生物工学講座 教授 

    2020年3月 - 現在

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    国名:日本国

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  • 九州大学 Faculty of Agriculture  

    2001年

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  • 東京大学農学部 助手   

    1989年

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  • 1989年4月東京大学農学部 1994年4月東京大学大学院農学生命科学研究科に配置換え   

学歴

  • 東京大学   農学系研究科   農芸化学

    - 1989年

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    国名:日本国

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  • 東京大学   農学部   農芸化学科

    - 1987年

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    国名:日本国

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研究テーマ・研究キーワード

  • 研究テーマ: 腸内細菌

    研究キーワード: 腸内細菌

    研究期間: 2024年

  • 研究テーマ: 微生物

    研究キーワード: 微生物

    研究期間: 2024年

  • 研究テーマ: 天然物有機化学

    研究キーワード: 天然物有機化学

    研究期間: 2024年

  • 研究テーマ: ペプチド

    研究キーワード: ペプチド

    研究期間: 2024年

  • 研究テーマ: バクテリオシン

    研究キーワード: バクテリオシン

    研究期間: 2024年

  • 研究テーマ: クオラムセンシング

    研究キーワード: クオラムセンシング

    研究期間: 2024年

  • 研究テーマ: Microbiology

    研究キーワード: Microbiology

    研究期間: 2024年

  • 研究テーマ: Bio-organic chemistry

    研究キーワード: Bio-organic chemistry

    研究期間: 2024年

  • 研究テーマ: アジア人の食と腸内フローラと健康に関する調査研究

    研究キーワード: 腸内細菌叢

    研究期間: 2010年4月

  • 研究テーマ: 腸内細菌叢の構造と機能に関する研究

    研究キーワード: 腸内細菌叢

    研究期間: 2010年4月

  • 研究テーマ: 醤油乳酸菌の分子シャペロンに関する研究

    研究キーワード: シャペロン、耐塩性

    研究期間: 2001年10月 - 2007年12月

  • 研究テーマ: 腸内細菌叢データを利用した乳幼児におけるアレルギー発症の早期診断法の確立

    研究キーワード: 腸内フローラ、アレルギー

    研究期間: 2001年10月

  • 研究テーマ: 発酵食品の細菌叢の解析

    研究キーワード: 糠床、細菌叢、分子生態学

    研究期間: 2001年10月

  • 研究テーマ: 乳酸菌の生産するバクテリオシンに関する研究

    研究キーワード: 乳酸菌、バクテリオシン

    研究期間: 2001年10月

  • 研究テーマ: 腸球菌の菌体外プロテアーゼの生産誘導フェロモンに関する基礎および応用研究

    研究キーワード: クォーラムセンシング

    研究期間: 1999年10月

  • 研究テーマ: ヒト腸内細菌のCell-cellコミュニケーション

    研究キーワード: クォーラムセンシング、プロバイオティクス、菌叢解析

    研究期間: 1998年4月

受賞

  • Topics of 2023

    2023年9月   日本生物工学会   糠床における複合微生物系における代謝コミュニティーのメタゲノム解析

  • 論文賞

    2020年9月   日本生物工学会   Dense tracking of the dynamics of the microbial community and chemicals constituents in spontaneous wheat sourdough during two months ofbackslopping

  • トピックス賞

    2018年3月   日本農芸化学会   日本人のインドネシア人の乳幼児の腸内メタゲノム比較解析

  • トピックス賞

    2018年3月   日本農芸化学会   日本人乳幼児の生後3年間の腸内フローラの形成・成熟化と胆汁酸代謝の関連性解析

  • トピックス賞

    2016年3月   日本農芸化学会   食物アレルギー発症児と非発症児の乳幼児期における腸内細菌叢の比較解析

  • トピックス賞

    2015年3月   日本農芸化学会   Asian Microbiome Project phase 2: アジア人の腸内細菌叢の加齢に伴う変化

  • 森永賞

    2008年6月   森永奉仕会   乳児アレルギー早期予知のための腸内フローラ解析技術の開拓

  • ポスター賞

    2005年8月   アメリカ微生物学会   Screening for quorum sensing inhibitors targeting fsr regulatory system

  • 論文賞

    2004年10月   日本生物工学会   Effect of Heterologous Expression of Molecular Chaperone DnaK from Tetragenococcus halophilus on Salinity Adaptation of Escherichia coli

  • 農芸化学奨励賞

    1999年4月   日本農芸化学会   腸球菌の生フェロモンシグナリングに関する生物有機化学的・分子生物学的研究

▼全件表示

論文

  • Gut microbiome status of urban and rural Filipino adults in relation to diet and metabolic disorders. 査読 国際誌

    Mai Watanabe, Abraham Sianoya, Riko Mishima, Phatthanaphong Therdtatha, Abigail Rodriguez, Donna Christene Ramos, Yuan Kun Lee, Leslie Michelle Dalmacio, Jiro Nakayama

    FEMS microbiology letters   368 ( 20 )   2021年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Here, we aim to understand the condition of the gut microbiome of Filipino adults in relation to their diet and metabolic status. Compared to rural Albay (n = 67), the gut microbiome of subjects living in urban Manila (n = 25) was more colonized by the order Clostridiales, which was negatively correlated with host carbohydrate consumption. Principal component analysis using the genus composition of the 92 total subjects indicated four microbiome types: one type driven by Prevotella, which was associated with high rice consumption and mainly consisted of healthy Albay subjects, one Clostridiales-driven group containing a number of type 2 diabetes mellitus (T2D) subjects from both Manila and Albay who showed lower butyrate levels in association with a decrease in Mediterraneibacter faecis, and the other two types showing dysbiosis-like microbiomes with Lactobacillus and Bifidobacterium overgrowth, with a high ratio of T2D and obese subjects. Multivariate logistic regression analysis suggested high dietary energy intake, and two Veillonellaeae genera, Dialister and Megasphaera, as T2D risk factors, while Prevotella and M. faecis as anti-T2D factors. In conclusion, low-carbohydrate diets restructured the Prevotella-driven gut microbiome, which may predispose Filipino people with high energy diet to T2D.

    DOI: 10.1093/femsle/fnab149

  • Gut Microbiome of Indonesian Adults Associated with Obesity and Type 2 Diabetes: A Cross-Sectional Study in an Asian City, Yogyakarta. 査読 国際誌

    Phatthanaphong Therdtatha, Yayi Song, Masaru Tanaka, Mariyatun Mariyatun, Maisaroh Almunifah, Nancy Eka Putri Manurung, Siska Indriarsih, Yi Lu, Koji Nagata, Katsuya Fukami, Tetsuo Ikeda, Yuan-Kun Lee, Endang Sutriswati Rahayu, Jiro Nakayama

    Microorganisms   9 ( 5 )   2021年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Indonesia is a developing country facing the national problem of the growing obesity and diabetes in its population due to recent drastic dietary and lifestyle changes. To understand the link between the gut microbiome, diet, and health of Indonesian people, fecal microbiomes and metabolomes of 75 Indonesian adults in Yogyakarta City, including obese people (n = 21), type 2 diabetes (T2D) patients (n = 25), and the controls (n = 29) were characterized together with their dietary and medical records. Variations of microbiomes showed a triangular distribution in the principal component analysis, driven by three dominant bacterial genera, namely Bacteroides, Prevotella, and Romboutsia. The Romboutsia-driven microbiome, characterized by low bacterial diversity and high primary bile acids, was associated with fat-driven obesity. The Bacteroides-driven microbiome, which counteracted Prevotella but was associated with Ruminococcaceae concomitantly increased with high-carbohydrate diets, showed positive correlation with T2D indices but negative correlation with body mass index. Notably, Bacteroides fragilis was increased in T2D patients with a decrease in fecal conjugated bile acids, particularly tauroursodeoxycholic acid (TUDCA), a farnesoid X receptor (FXR) antagonist with anti-diabetic activity, while these features disappeared in patients administered metformin. These results indicate that the gut microbiome status of Indonesian adults is differently associated with obesity and T2D under their varied dietary habits.

    DOI: 10.3390/microorganisms9050897

  • Cultural isolation of spore-forming bacteria in human feces using bile acids. 査読 国際誌

    Masaru Tanaka, Sakura Onizuka, Riko Mishima, Jiro Nakayama

    Scientific reports   10 ( 1 )   15041 - 15041   2020年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Structurally-diversified bile acids (BAs) are involved in shaping of intestinal microbiota as well as absorption of dietary lipids. Taurocholic acid, a conjugated form of BA, has been reported to be a factor triggering germination of a wide range of spore-forming bacteria in intestine. To test a hypothesis that other BAs also promote germination of intestinal bacteria, we attempted culture of bacteria from ethanol-treated feces by using a series of BAs. It was found that conjugated-BAs, notably three glycine-conjugated BAs, glycodeoxycholic acid and glycochenodeoxycholic acid, significantly increased the number and the species variety of colonies formed on the agar plate. These colonized bacteria mostly belonged to class Clostridia, mainly consisting of families Lachnospiraceae, Clostridiaceae, and Peptostreptococcaceae. There were several types of bacteria associated with different sensitivity to each BA. Eventually, we isolated 72 bacterial species of which 61 are known and 11 novel. These results demonstrate that the culturable range of bacteria in intestine can be widened using the germination-inducing activity of BAs. This approach would advance the research on spore-forming Clostridia that contains important but difficult-to-cultured bacteria associate with host health and diseases.

    DOI: 10.1038/s41598-020-71883-1

  • The association between gut microbiota development and maturation of intestinal bile acid metabolism in the first 3 y of healthy Japanese infants 査読 国際誌

    Masaru Tanaka, Masafumi Sanefuji, Seiichi Morokuma, Misako Yoden, Rie Momoda, Kenji Sonomoto, Masanobu Ogawa, Kiyoko Kato, Jiro Nakayama

    Gut Microbes   2 ( 11 )   205 - 216   2020年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    The gut microbial community greatly changes in early life, influencing infant health and subsequent host physiology, notably through its collective metabolism, including host–microbiota interplay of bile acid (BA) metabolism. However, little is known regarding how the development of the intestinal microbial community is associated with maturation of intestinal BA metabolism. To address this, we monitored the succession of gut bacterial community and its association with fecal BA profile in the first 3 y of ten healthy Japanese infants. The BA profiles were classified into four types, defined by high content of conjugated primary BA (Con type), unconjugated primary BA (chenodeoxycholic acid and cholic acid) (Pri type), ursodeoxycholic acid (Urs type), and deoxycholic and lithocholic acid (Sec type). Most subjects begun with Con type or Pri type profiles during lactation and eventually transited to Sec type through Urs type after the start of solid food intake. Con type and Pri type were associated with Enterobacteriaceae-dominant microbiota corresponding to the neonatal type or Bifidobacterium-dominant microbiota corresponding to lactation type, respectively. Urs type subjects were strongly associated with Ruminococcus gnavus colonization, mostly occurring between Pri type and Sec type. Sec type was associated with adult-type complex microbiota dominated by a variety of Firmicutes and Bacteroidetes species. Addressing the link of the common developmental passage of intestinal BA metabolism with infant’s health and subsequent host physiology requires further study.

    DOI: 10.1080/19490976.2019.1650997

  • Lowering effect of viable Pediococcus pentosaceus QU 19 on the rise in postprandial glucose 査読 国際誌

    Miki Fujiwara, Daichi Kuwahara, Masahiro Hayashi, Takeshi Zendo, Masao Sato, Jiro Nakayama, Kenji Sonomoto

    Bioscience of Microbiota, Food and Health   39 ( 2 )   57 - 64   2020年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    In the present study, we investigated the glucose-decreasing action of lactic acid bacteria (LAB). The finding of this study could be helpful for people in controlling their blood sugar levels. The LAB candidate was isolated from a Japanese fermented food and identified as Pediococcus pentosaceus by an analysis of its genome sequence. Postprandial blood glucose elevation was investigated using oral starch tolerance tests in mice. Normal mice were fed starch and lyophilized cells of P. pentosaceus QU 19 at the same time. Even without pre-administration of P. pentosaceus QU 19, elevation of the blood glucose level was significantly suppressed by the intake of P. pentosaceus QU 19 at the same time as oral administration of starch. According to the results for its survival in simulated digestive juice and the reduction of blood glucose level in mice, P. pentosaceus QU 19 has potential hypoglycemic activity. In vitro measurements revealed that the glucose-decreasing action of P. pentosaceus QU 19 is probably caused by the glucose assimilation of the strain, not the inhibition of carbohydrate-splitting enzymes which has been reported for other LABs previously. These findings indicate that specific strains of LAB, especially P. pentosaceus QU 19, and foods fermented by LAB may be beneficial for people who must manage glucose ingestion.

    DOI: 10.12938/BMFH.19-041

  • Dense tracking of the dynamics of the microbial community and chemicals constituents in spontaneous wheat sourdough during two months of backslopping. 査読

    Mugihito Oshiro, Rie Momoda, Masaru Tanaka, Takeshi Zendo, Jiro Nakayama

    Journal of bioscience and bioengineering   128 ( 2 )   170 - 176   2019年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Wheat sourdough is a common traditional fermented food that is produced worldwide. However, product quality of spontaneous sourdough is not easy to control because it depends on natural fermentation and backslopping, about which little is known, notably after ten backslopping steps. To this end, we tracked the spontaneous fermentation of three sourdoughs made from wheat flours during 32 backslopping steps for 60 days. At 24 time points, the microbial community was analyzed by both culture-dependent and culture-independent methods and its chemical constituents were assessed. Dynamic changes were observed in the microbial community, which showed a common succession pattern among the three sourdoughs at the bacterial family level and differences at the species level. The bacterial communities evolved through three phases that were driven by different groups of lactic acid bacteria (LAB) species. The dynamism among the metabolites also differed, depending on the species composition of the LAB and yeast communities. In one sourdough, the growth of Saccharomyces cerevisiae was detected along with a concentration of increased ethanol, while in the other two sourdoughs, Wickerhamomyces anomalus was detected without ethanol production. Regarding the LAB communities, two sourdoughs were eventually co-dominated by Lactobacillus plantarum and L. brevis, while the other sourdough was eventually dominated solely by the heterolactic fermentative bacterium Lactobacillus fermentum, and ethanol was produced at the same level as lactic acid. Further research is needed to determine the bacterial and yeast species involved in the fermentation of sourdough, to help improve the design and quality control of the final product.

    DOI: 10.1016/j.jbiosc.2019.02.006

  • Urban Diets Linked to Gut Microbiome and Metabolome Alterations in Children: A Comparative Cross-sectional Study in Thailand 査読 国際誌

    Juma M. Kisuse, Orawan La-ongkham, Massalin Nakphaichit, Phatthanaphong Therdtatha, Rie Momoda, Masaru Tanaka, Shinji Fukuda, Siam Popluechai, Kongkiat Kespechara, Kenji Sonomoto, Yuan-Kun Lee, Sunee Nitisinprasert and Jiro Nakayama

    Front. Microbiol.   2018年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.3389/fmicb.2018.01345

  • Gut dysbiosis associated with hepatitis C virus infection 査読 国際誌

    Inoue T, Nakayama J, Moriya K, Kawaratani H, Momoda R, Ito K, Iio E, Nojiri S, Fujiwara K, Yoneda M, Yoshiji H, Tanaka Y

    Clin Infect Dis   67 ( 6 )   869 - 877   2018年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Background:Little is known about the effect of hepatitis C virus (HCV) infection on gut microbiota and the relationship between alteration of gut microbiota and chronic hepatitis C (CHC) progression. We performed a comparative study of gut microbiota composition between CHC patients and healthy individuals.

    Methods:Fecal samples from 166 CHC patients were compared with those from 23 healthy individuals; the gut microbiota community was analyzed using 16S ribosomal RNA gene sequencing. CHC patients were diagnosed with persistently normal serum alanine aminotransferase without evidence of liver cirrhosis (LC) (PNALT, n = 18), chronic hepatitis (CH, n = 84), LC (n = 40), and hepatocellular carcinoma in LC (n = 24).

    Results:Compared with healthy individuals, bacterial diversity was lower in persons with HCV infection, with a decrease in the order Clostridiales and an increase in Streptococcus and Lactobacillus. Microbiota dysbiosis already appeared in the PNALT stage with the transient increase in Bacteroides and Enterobacteriaceae. Predicted metagenomics of microbial communities showed an increase in the urease gene mainly encoded by viridans streptococci during CHC progression, consistent with a significantly higher fecal pH in CH and LC patients than in healthy individuals or those in the PNALT stage.

    Conclusions:HCV infection is associated with gut dysbiosis, even in patients with mild liver disease. Additionally, overgrowth of viridans streptococci can account for hyperammonemia in CH and LC. Further studies would help to propose a novel treatment strategy because the gut microbiome can be therapeutically altered, potentially reducing the complications of chronic liver disease.

    DOI: 10.1093/cid/ciy205

  • Metabolic dependent and independent pH-drop shuts down VirSR quorum sensing in Clostridium perfringens. 査読

    Keika Adachi, Kaori Ohtani, Michio Kawano, Ravindra Pal Singh, Basit Yousuf, Kenji Sonomoto, Tohru Shimizu, Jiro Nakayama

    Journal of bioscience and bioengineering   125 ( 5 )   525 - 531   2018年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Clostridium perfringens produces various exotoxins and enzymes that cause food poisoning and gas gangrene. The genes involved in virulence are regulated by the agr-like quorum sensing (QS) system, which consists of a QS signal synthesis system and a VirSR two-component regulatory system (VirSR TCS) which is a global regulatory system composed of signal sensor kinase (VirS) and response regulator (VirR). We found that the perfringolysin O gene (pfoA) was transiently expressed during mid-log phase of bacterial growth; its expression was rapidly shut down thereafter, suggesting the existence of a self-quorum quenching (sQQ) system. The sQQ system was induced by the addition of stationary phase culture supernatant (SPCS). Activity of the sQQ system was heat stable, and was present following filtration through the ultrafiltration membrane, suggesting that small molecules acted as sQQ agents. In addition, sQQ was also induced by pure acetic and butyric acids at concentrations equivalent to those in the stationary phase culture, suggesting that organic acids produced by C. perfringens were involved in sQQ. In pH-controlled batch culture, sQQ was greatly diminished; expression level of pfoA extended to late-log growth phase, and was eventually increased by one order of magnitude. Furthermore, hydrochloric acid induced sQQ at the same pH as was used in organic acids. SPCS also suppressed the expression of genes regulated by VirSR TCS. Overall, the expression of virulence factors of C. perfringens was downregulated by the sQQ system, which was mediated by primary acidic metabolites and acidic environments. This suggested the possibility of pH-controlled anti-virulence strategies.

    DOI: 10.1016/j.jbiosc.2017.12.019

  • Development of the gut microbiota in infancy and its impact on health in later life. 招待 査読 国際誌

    Masaru Tanaka, Jiro Nakayama

    Allergology international : official journal of the Japanese Society of Allergology   66 ( 4 )   515 - 522   2017年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Gut microbial ecology and function are dynamic in infancy, but are stabilized in childhood. The 'new friends' have a great impact on the development of the digestive tract and host immune system. In the first year of life, especially, the gut microbiota dramatically changes through interactions with the developing immune system in the gut. The process of establishing the gut microbiota is affected by various environmental factors, with the potential to be a main determinant of life-long health. In this review, we summarize recent findings regarding gut microbiota establishment, including the importance of various factors related to the development of the immune system and allergic diseases later in life.

    DOI: 10.1016/j.alit.2017.07.010

  • Signatures in the gut microbiota of Japanese infants who developed food allergies in early childhood. 査読 国際誌

    Masaru Tanaka, Yuki Korenori, Masakazu Washio, Takako Kobayashi, Rie Momoda, Chikako Kiyohara, Aki Kuroda, Yuka Saito, Kenji Sonomoto, Jiro Nakayama

    FEMS microbiology ecology   93 ( 8 )   1 - 11   2017年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Bacterial colonization in infancy is considered crucial for the development of the immune system. Recently, there has been a drastic increase in childhood allergies in Japan. Therefore, we conducted a prospective study with 56 infants on the relationship between gut microbiota in the first year of life and the development of allergies during the first 3 years. In the lactation period, organic acid producers such as Leuconostoc, Weissella and Veillonella tended to be underrepresented in subjects who developed food allergies (FA, n = 14) within the first two years. In the weaning period, children in the FA group were highly colonized by unclassified Enterobacteriaceae and two Clostridium species closely related to Clostridium paraputrificum and C. tertium, and the whole tree phylogenetic diversity index was significantly lower in the FA group. All of these differences in the weaning period were statistically significant, even after adjusting for potential confounding factors. A higher abundance of unclassified Enterobacteriaceae was also found in the other allergic group (n = 15), whereas the two Clostridium species were highly specific to the FA group. The mode of action of these Clostridium species in childhood food allergies remains unknown, warranting further investigation.

    DOI: 10.1093/femsec/fix099

  • Impact of westernized diet on gut microbiota in children on Leyte island 査読

    Jiro Nakayama, Azusa Yamamoto, Ladie A. Palermo-Conde, Kanako Higashi, Kenji Sonomoto, Julie Tan, Yuan Kun Lee

    Frontiers in Microbiology   8 ( FEB )   2017年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Urbanization has changed life styles of the children in some towns and cities on Leyte island in the Philippines. To evaluate the impact of modernization in dietary habits on gut microbiota, we compared fecal microbiota of 7 to 9-year-old children from rural Baybay city (n = 24) and urban Ormoc city (n = 19), and assessed the correlation between bacterial composition and diet. A dietary survey indicated that Ormoc children consumed fast food frequently and more meat and confectionary than Baybay children, suggesting modernization/westernization of dietary habits. Fat intake accounted for 27.2% of the total energy intake in Ormoc children; this was remarkably higher than in their Baybay counterparts (18.1%) and close to the upper limit (30%) recommended by the World Health Organization. Their fecal microbiota were analyzed by high-throughput 16S rRNA gene sequencing in conjunction with a dataset from five other Asian countries. Their microbiota were classified into two enterotype-like clusters with the other countries' children, each defined by high abundance of either Prevotellaceae (P-type) or Bacteroidaceae (BB-type), respectively. Baybay and Ormoc children mainly harbored P-type and BB-type, respectively. Redundancy analysis showed that P-type favored carbohydrates whereas BB-type preferred fats. Fat intake correlated positively with the Firmicutes-to-Bacteroidetes (F/B) ratio and negatively with the relative abundance of the family Prevotellaceae/genus Prevotella. A species-level analysis suggested that dietary fat positively correlated with an Oscillibacter species as well as a series of Bacteroides/Parabacteroides species, whereas dietary carbohydrate positively correlated with Dialister succinatiphilus known as succinate-utilizing bacteria and some succinate-producing species of family Prevotellaceae, Veillonellaceae, and Erysipelotrichaceae. We also found that a Succinivibrio species was overrepresented in the P-type community, suggesting the syntroph via hydrogen and succinate. Predicted metagenomics suggests that BB-type microbiota is well nourished and metabolically more active with simple sugars, amino acids, and lipids, while P-type community is more involved in digestion of complex carbohydrates. Overweight and obese children living in Ormoc, who consumed a high-fat diet, harbored microbiota with higher F/B ratio and low abundance of Prevotella. The altered gut microbiota may be a sign of a modern diet-associated obesity among children in developing areas.

    DOI: 10.3389/fmicb.2017.00197

  • Fermented papaya preparation restores age- related reductions in peripheral blood mononuclear cell cytolytic activity in tube- fed patients 査読

    Yuhzo Fujita, Haruo Tsuno, Jiro Nakayama

    PLoS One   12 ( 1 )   2017年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Tube-fed elderly patients are generally supplied with the same type of nutrition over long periods, resulting in an increased risk for micronutrient deficiencies. Dietary polyphenols promote immunity and have anti-inflammatory, anti-carcinogenic, and anti-oxidative properties. Carica papaya Linn. is rich in several polyphenols; however, these polyphenols are poorly absorbed from the digestive tract in their original polymerized form. Therefore, we determined the molecular components of a fermented Carica papaya Linn. preparation, as well as its effects on immunity and the composition of gut microbiota in tube-fed patients. Different doses of the fermented C. papaya L. preparation were administered to three groups of tube-fed patients for 30 days. Its effects on fecal microbiota composition and immunity were assessed by 16S rRNA gene sequencing and immune-marker analysis, respectively. The chemical composition of the fermented C. papaya L. preparation was analyzed by capillary electrophoresisand liquid chromatography- time of flight mass spectrometry. The fermented C. papaya L. preparation restored peripheral blood mononuclear cell (PBMC) cytolytic activity; however, no other biomarkers of immunity were observed. Treatment with the preparation (9 g/day) significantly reduced the abundance of Firmicutes in the fecal microbiota. In particular, treatment reduced Clostridium scindens and Eggerthella lenta in most patients receiving 9 g/day. Chemical analysis identified low-molecular-weight phenolic acids as polyphenol metabolites; however, no polymerized, large-molecular-weight molecules were detected. Our study indicates that elderly patients who are tube-fed over the long-term have decreased PBMC cytolytic activity. In addition, low-molecular-weight polyphenol metabolites fermented from polymerized polyphenols restore PBMC cytolytic activity and modulate the composition of gut microbiota in tube-fed patients.

    DOI: 10.1371/journal.pone.0169240

  • Microbial Community of Healthy Thai Vegetarians and Non-Vegetarians, Their Core Gut Microbiota, and Pathogen Risk. 査読 国際誌

    Supatjaree Ruengsomwong, Orawan La-Ongkham, Jiahui Jiang, Bhusita Wannissorn, Jiro Nakayama, Sunee Nitisinprasert

    Journal of microbiology and biotechnology   26 ( 10 )   1723 - 1735   2016年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Pyrosequencing analysis of intestinal microflora from healthy Thai vegetarians and non-vegetarians exhibited 893 OTUs covering 189 species. The strong species indicators of vegetarians and non-vegetarians were Prevotella copri and Bacteroides vulgatus as well as bacteria close to Escherichia hermanii with % relative abundance of 16.9 and 4.5-4.7, respectively. Core gut microbiota of the vegetarian and non-vegetarian groups consisted of 11 and 20 different bacterial species, respectively, belonging to Actinobacteria, Firmicutes, and Proteobacteria commonly found in both groups. Two species, Faecalibacterium prausnitzii and Gemmiger formicilis, had a prevalence of 100% in both groups. Three species, Clostridium nexile, Eubacterium eligens, and P. copri, showed up in most vegetarians, whereas more diversity of Collinsella aerofaciens, Ruminococcus torques, various species of Bacteroides, Parabacteroides, Escherichia, and different species of Clostridium and Eubacterium were found in most non-vegetarians. Considering the correlation of personal characters, consumption behavior, and microbial groups, the age of non-vegetarians showed a strong positive correlation coefficient of 0.54 (p = 0.001) to Bacteroides uniformis but exhibited a moderate one to Alistipes finegoldii and B. vulgatus. Only a positive moderate correlation of body mass index and Parabacteroides distasonis appeared. Based on the significant abundance of potential pathogens, the microbiota of the non-vegetarian group showed an abundance of potential pathogen varieties of Bilophila wadsworthia, Escherichia coli, and E. hermannii, whereas that of the vegetarian group served for only Klebsiella pneumoniae. These results implied that the microbiota of vegetarians with high abundance of P. copri and low potential pathogen variety would be a way to maintain good health in Thais.

    DOI: 10.4014/jmb.1603.03057

  • Avellanin C, an inhibitor of quorum-sensing signaling in Staphylococcus aureus, from Hamigera ingelheimensis. 査読

    Yasuhiro Igarashi, Fumiya Gohda, Taito Kadoshima, Takao Fukuda, Tomoaki Hanafusa, Akane Shojima, Jiro Nakayama, Gerald F Bills, Stephen Peterson

    The Journal of antibiotics   68 ( 11 )   707 - 710   2015年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1038/ja.2015.50

  • Diversity in gut bacterial community of school-age children in Asia. 査読 国際誌

    Jiro Nakayama, Koichi Watanabe, Jiahui Jiang, Kazunori Matsuda, Shiou-Huei Chao, Pri Haryono, Orawan La-Ongkham, Martinus-Agus Sarwoko, I Nengah Sujaya, Liang Zhao, Kang-Ting Chen, Yen-Po Chen, Hsueh-Hui Chiu, Tomoko Hidaka, Ning-Xin Huang, Chikako Kiyohara, Takashi Kurakawa, Naoshige Sakamoto, Kenji Sonomoto, Kousuke Tashiro, Hirokazu Tsuji, Ming-Ju Chen, Vichai Leelavatcharamas, Chii-Cherng Liao, Sunee Nitisinprasert, Endang S Rahayu, Fa-Zheng Ren, Ying-Chieh Tsai, Yuan-Kun Lee

    Scientific reports   5   8397 - 8397   2015年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Asia differs substantially among and within its regions populated by diverse ethnic groups, which maintain their own respective cultures and dietary habits. To address the diversity in their gut microbiota, we characterized the bacterial community in fecal samples obtained from 303 school-age children living in urban or rural regions in five countries spanning temperate and tropical areas of Asia. The microbiota profiled for the 303 subjects were classified into two enterotype-like clusters, each driven by Prevotella (P-type) or Bifidobacterium/Bacteroides (BB-type), respectively. Majority in China, Japan and Taiwan harbored BB-type, whereas those from Indonesia and Khon Kaen in Thailand mainly harbored P-type. The P-type microbiota was characterized by a more conserved bacterial community sharing a greater number of type-specific phylotypes. Predictive metagenomics suggests higher and lower activity of carbohydrate digestion and bile acid biosynthesis, respectively, in P-type subjects, reflecting their high intake of diets rich in resistant starch. Random-forest analysis classified their fecal species community as mirroring location of resident country, suggesting eco-geographical factors shaping gut microbiota. In particular, children living in Japan harbored a less diversified microbiota with high abundance of Bifidobacterium and less number of potentially pathogenic bacteria, which may reflect their living environment and unique diet.

    DOI: 10.1038/srep08397

  • Cyclodepsipeptides produced by actinomycetes inhibit cyclic-peptide-mediated quorum sensing in Gram-positive bacteria 査読

    Said E. Desouky, Akane Shojima, Ravindra Pal Singh, Takahisa Matsufuji, Yasuhiro Igarashi, Takashi Suzuki, Tohru Yamagaki, Ken Ichi Okubo, Kaori Ohtani, Kenji Sonomoto, Jiro Nakayama

    FEMS Microbiology Letters   362 ( 14 )   2015年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Cyclic peptides are commonly used as quorum-sensing autoinducers in Gram-positive Firmicutes bacteria. Well-studied examples of such molecules are thiolactone and lactone, used to regulate the expression of a series of virulence genes in the agr system of Staphylococcus aureus and the fsr system of Enterococcus faecalis, respectively. Three cyclodepsipeptides WS9326A,WS9326B and cochinmicin II/III were identified as a result of screening actinomycetes culture extracts for activity against the agr/fsr system. These molecules are already known as receptor antagonists, the first two for tachykinin and the last one for endothelin. WS9326A also inhibited the transcription of pfoA regulated by the VirSR two-component system in Clostridium perfringens. Receptor-binding assays using a fluorescence-labeled autoinducer (FITC-GBAP) showed that WS9326A and WS9326B act as receptor antagonists in this system. In addition, an ex vivo assay showed that WS9326B substantially attenuated the toxicity of S. aureus for human corneal epithelial cells. These results suggest that these three natural cyclodepsipeptides have therapeutic potential for targeting the cyclic peptide-mediated quorum sensing of Gram-positive pathogens.

    DOI: 10.1093/femsle/fnv109

  • Rationale design of quorum-quenching peptides that target the VirSR system of Clostridium perfringens 査読

    Ravindra Pal Singh, Ken Ichi Okubo, Kaori Ohtani, Keika Adachi, Kenji Sonomoto, Jiro Nakayama

    FEMS Microbiology Letters   362 ( 22 )   2015年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    In Clostridium perfringens, a 5-membered thiolactone peptide acts as an autoinducing peptide (AIPCp) to activate the VirSR two-component signal transduction system, which in turn controls the expression of genes encoding multiple toxins, including α, θ and κ. To develop anti-pathogenic agents against virulent C. perfringens, quorum-quenching peptides were rationally designed based on the structure-activity relationship (SAR) data on AIPCp. Alanine scanning study of AIPCp suggested that Trp3 and Phe4 are involved in receptor binding and activation, respectively. On the basis of the SAR, we designed two quorum-quenching peptides with different modes of action: Z-AIPCp-L2A/T5A (partial agonist) and Z-AIPCp-F4A/T5S (partial antagonist). Both peptides significantly attenuated transcription of θ toxin gene (pfoA) in a virulent strain of C. perfringens with IC50 = 0.32 and 0.72 μM, respectively.

    DOI: 10.1093/femsle/fnv188

  • Monitoring of the microbiota profile in nukadoko, a naturally fermented rice bran bed for pickling vegetables 査読

    Hiroshi Ono, Shoko Nishio, Jun Tsurii, Tetsuhiro Kawamoto, Kenji Sonomoto, Jiro Nakayama

    Journal of Bioscience and Bioengineering   118 ( 5 )   520 - 525   2014年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Nukadoko is a fermented rice bran mash traditionally used for pickling vegetables in Japan. To date, the production of both homemade and commercial nukadoko depends on natural fermentation without using starter cultures. Here, we monitored chemical and microbiological changes in the initial batch fermentation of nukadoko. Nukadoko samples were prepared by spontaneous fermentation of four different brands of rice bran, and microbiome dynamics were analyzed for 2 months. In the first week, non-Lactobacillales lactic acid bacteria (LAB) species, which differed among the samples, grew proportionally to pH decrease and lactate increase. Thereafter, Lactobacillus plantarum started growing and consumed residual sugars, causing further lactate increase in nukadoko. Finally, microbial communities in all tested nukadoko samples were dominated by L.plantarum. Taken together, our results suggest that the mixture of the fast-growing LAB species and slow-growing L.plantarum may be used as a suitable starter culture to promote the initial fermentation of nukadoko.

    DOI: 10.1016/j.jbiosc.2014.04.017

  • Quorum sensing in gram-positive bacteria Assay protocols for staphylococcal agr and enterococcal fsr systems 査読

    Akane Shojima, Jiro Nakayama

    Methods in Molecular Biology   1147   33 - 41   2014年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    A thiolactone/lactone peptide-mediated quorum sensing (QS) system is commonly employed in gram- positive bacteria to control the expression of a variety of phenotypes, including the production of virulence factors and biofilm formation. Here, we describe assay protocols for the well-studied QS systems (agr and fsr) of two representative gram-positive pathogens, Staphylococcus aureus and Enterococcus faecalis . These convenient assay systems are useful for the screening of QS inhibitors as well as for basic research to address the mechanism of these QS systems.

    DOI: 10.1007/978-1-4939-0467-9_3

  • High-throughput screening of inhibitors targeting Agr/Fsr quorum sensing in staphylococcus aureus and enterococcus faecalis 査読

    Said E. Desouky, Kenzo Nishiguchi, Takeshi Zendo, Yasuhiro Igarashi, Paul Williams, Kenji Sonomoto, Jiro Nakayama

    Bioscience, Biotechnology and Biochemistry   77 ( 5 )   923 - 927   2013年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Staphylococcus aureus and Enterococcus faecalis employ cyclic peptide-mediated quorum sensing (QS) systems, termed agr and fsr respectively, to regulate the expression of a series of virulence genes. To identify quorum sensing inhibitors (QSIs) that target agr/fsr systems, an efficient screening system was established. In addition to the gelatinase-induction assay to examine E. faecalis fsr QS, the use of an S. aureus agr reporter strain that carries luciferase and green fluorescence protein genes under the agr P3 promoter facilitated the development of a high-throughput screen (HTS) for QSIs. As a result of screening of 906 actinomycetes culture extracts, four showed QSI activity against the agr and fsr systems without growth inhibitory activity. The extracts were purified on a small scale, and three HPLC peaks were obtained with obvious QSI activity. In sum, the established HTS system is a promising strategy for the discovery of anti-pathogenic agents targeting cyclic peptide-mediated QS in Gram-positive pathogens.

    DOI: 10.1271/bbb.120769

  • Development of a peptide antagonist against fsr quorum sensing of Enterococcus faecalis. 査読 国際誌

    Jiro Nakayama, Ryoji Yokohata, Mami Sato, Takashi Suzuki, Takahisa Matsufuji, Kenzo Nishiguchi, Takeshi Kawai, Yosuke Yamanaka, Koji Nagata, Masaru Tanokura, Kenji Sonomoto

    ACS chemical biology   8 ( 4 )   804 - 811   2013年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Enterococcus faecalis fsr quorum sensing (QS) involves an 11-residue cyclic peptide named gelatinase biosynthesis-activating pheromone (GBAP) that autoinduces two pathogenicity-related extracellular proteases in a cell density-dependent fashion. To identify anti-pathogenic agents that target fsr QS signaling, peptide antagonists of GBAP were created by our unique drug design approach based on reverse alanine scanning. First of all, a receptor-binding scaffold (RBS), [Ala(4,5,6,8,9,11)]Z-GBAP, was created, in which all amino acids within the ring region of GBAP, except for two essential aromatic residues, were substituted to alanine. Next, the substituted alanine residues were changed back to the original amino acid one by one, permitting selection of those peptide combinations exhibiting increased antagonist activity. After three cycles of this reverse alanine scan, [Ala(5,9,11)]Z-GBAP was obtained as a maximally reverted peptide (MRP) holding the strongest antagonist activity. Then, the fifth residue in MRP, which is one of the critical residues to determine agonist/antagonist activity, was further modified by substituting with different types of amino acids including unnatural amino acids. As a result, [Tyr(Bzl)(5), Ala(9,11)]Z-GBAP, named ZBzl-YAA5911, showed the strongest antagonist activity [IC(50) = 26.2 nM and Kd against GBAP receptor (FsrC) = 39.4 nM]. In vivo efficacy of this peptide was assessed with an aphakic rabbit endophthalmitis model. ZBzl-YAA5911 suppressed the translocation of E. faecalis from the aqueous humor into the vitreous cavity by more than 1 order of magnitude and significantly reduced retinal damage. We propose that ZBzl-YAA5911 or its derivatives would be useful as anti-infective agents to attenuate virulence expression in this opportunistic pathogen.

    DOI: 10.1021/cb300717f

  • Aberrant structures of fecal bacterial community in allergic infants profiled by 16S rRNA gene pyrosequencing. 査読 国際誌

    Jiro Nakayama, Takako Kobayashi, Shigemitsu Tanaka, Yuki Korenori, Atsushi Tateyama, Naoshige Sakamoto, Chikako Kiyohara, Taro Shirakawa, Kenji Sonomoto

    FEMS immunology and medical microbiology   63 ( 3 )   397 - 406   2011年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    We investigated the correlation between fecal bacteria composition in early infancy and the prevalence of allergic diseases in late infancy. The fecal microbiota in the first 2 months was profiled using the 16S rRNA V6 short-tag sequences in the community and statistically compared between two groups of subjects who did and did not show allergic symptoms in the first 2 years (n = 11 vs. 11). In the allergic group, genus Bacteroides at 1 month and genera Propionibacterium and Klebsiella at 2 months were more abundant, and genera Acinetobacter and Clostridium at 1 month were less abundant than in the nonallergic group. Allergic infants who showed high colonization of Bacteroides and/or Klebsiella showed less colonization of Clostridium perfringens/butyricum, suggesting antagonism between these bacterial groups in the gastrointestinal tract. It was also remarkable that the relative abundance of total Proteobacteria, excluding genus Klebsiella, was significantly lower in the allergic than in the nonallergic group at the age of 1 month. These results indicate that pyrosequence-based 16S rRNA gene profiling is valid to find the intestinal microbiotal disorder that correlates with allergy development in later life.

    DOI: 10.1111/j.1574-695X.2011.00872.x

  • 16S rRNA pyrosequencing-based investigation of the bacterial community in nukadoko, a pickling bed of fermented rice bran. 査読 国際誌

    Naoshige Sakamoto, Shigemitsu Tanaka, Kenji Sonomoto, Jiro Nakayama

    International journal of food microbiology   144 ( 3 )   352 - 359   2011年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Nukadoko is a naturally fermented rice bran mash traditionally used for pickling vegetables in Japan; its refreshment and fermentation cycles sometimes continue for many years. Here, we investigated the structure and dynamics of the bacterial community in nukadoko by conducting pyrosequencing and quantitative polymerase chain reaction (PCR) analyses of 16S ribosomal RNA genes (rDNA). Of the 16 different samples studied, 13 showed Lactobacillus-dominated microbiota, suggesting that aged nukadoko samples tend to realize a niche, favorable Lactobacillus species. The lactic acid bacterial community of each of the 16 samples was classified into 3 types according to the presence or absence of 2 predominant species, Lactobacillus namurensis and Lactobacillus acetotolerans. The dynamics of the bacterial community during fermentation and the subsequent ripening process were examined using a laboratory model of nukadoko inoculated with an aged nukadoko sample (inoculated model). Lb. namurensis grew rapidly in the first 2 days, accompanied with a rapid decrease in pH and an increase in lactate levels, while Lb. acetotolerans grew with a longer doubling time and slow acidification during the 20 days after inoculation. On the other hand, spontaneous fermentation of the nukadoko model prepared from fresh rice bran without the nukadoko inoculation (inoculant-free model), showed the growth of some non-Lactobacillus species such as staphylococci and bacilli within the first 10 days; thereafter, Lb. namurensis was dominant, while Lb. acetotolerans was not detected during the 20-day experimental period. These results suggest that the naturally established Lactobacillus community in aged nukadoko is effectively involved in the biocontrol of the microbial community of nukadoko during the refreshment and fermentation cycles.

    DOI: 10.1016/j.ijfoodmicro.2010.10.017

  • Ambuic acid inhibits the biosynthesis of cyclic peptide quormones in gram-positive bacteria. 査読 国際誌

    Jiro Nakayama, Yumi Uemura, Kenzo Nishiguchi, Norito Yoshimura, Yasuhiro Igarashi, Kenji Sonomoto

    Antimicrobial agents and chemotherapy   53 ( 2 )   580 - 586   2009年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Quorum sensing is a cell-density-dependent regulatory system in gram-positive bacteria and is often regulated by cyclic peptides called "quormones," which function as extracellular communication signals. With an aim to discover an antipathogenic agent targeting quorum sensing in gram-positive bacteria, we screened 153 samples of fungal butanol extracts with the guidance of the inhibition of quorum-sensing-mediated gelatinase production in Enterococcus faecalis. Following the screenings, we found that ambuic acid, a known secondary fungal metabolite, inhibited the quorum-sensing-mediated gelatinase production without influencing the growth of E. faecalis. We further demonstrated that ambuic acid targeted the biosynthesis of a cyclic peptide quormone called gelatinase biosynthesis-activating pheromone. Furthermore, ambuic acid also inhibited the biosynthesis of the cyclic peptide quormones of Staphylococcus aureus and Listeria innocua. These results suggest the potential use of ambuic acid as a lead compound of antipathogenic drugs that target the quorum-sensing-mediated virulence expression of gram-positive bacteria.

    DOI: 10.1128/AAC.00995-08

  • Structure-activity relationship of gelatinase biosynthesis-activating pheromone of Enterococcus faecalis. 査読 国際誌

    Kenzo Nishiguchi, Koji Nagata, Masaru Tanokura, Kenji Sonomoto, Jiro Nakayama

    Journal of bacteriology   191 ( 2 )   641 - 650   2009年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    The expression of pathogenicity-related extracellular proteases, namely, gelatinase and serine protease, in Enterococcus faecalis is positively regulated by a quorum-sensing system mediated by an autoinducing peptide called gelatinase biosynthesis-activating pheromone (GBAP). GBAP is an 11-amino-acid-residue cyclic peptide containing a lactone linkage. To study the structure-activity relationship of GBAP, we synthesized a series of GBAP analogues and evaluated their activities by a gelatinase-inducing assay and newly developed receptor-binding assays in which fluorescence-labeled peptides bound onto the FsrC-overexpressing Lactococcus lactis cell surface were observed by fluorescent microscopy and quantified by using a fluorophotometer. Alanine-scanning analysis of GBAP showed that the entire ring region was involved in the GBAP agonist activity, while side chains of the tail region were not strictly recognized. The alanine substitution of Phe(7) or Trp(10) almost abolished their receptor-binding abilities and GBAP agonist activities, suggesting that these two aromatic side chains are strongly involved in receptor interaction and activation. Furthermore, the Trp(10) substitution with natural and unnatural aromatic amino acids, except pentafluorophenylalanine, caused no loss of agonist activity. This suggested the importance of a negative electrostatic potential created by an pi-electron cloud on the aromatic ring surface. Structural analysis of GBAP with nuclear magnetic resonance spectroscopy revealed that the ring region adopted a hairpin-like fold and was tightly packed into a compact form. The side chain of Trp(10) was partially buried in the core structure, contributing to the stabilization of the compact form, while that of Phe(7) was extended from the core structure into the solvent and was probably directly involved in receptor binding.

    DOI: 10.1128/JB.01029-08

  • Two homologous agr-like quorum-sensing systems cooperatively control adherence, cell morphology, and cell viability properties in Lactobacillus plantarum WCFS1 査読

    Toshio Fujii, Colin Ingham, Jiro Nakayama, Marke Beerthuyzen, Ryoko Kunuki, Douwe Molenaar, Mark Sturme, Elaine Vaughan, Michiel Kleerebezem, Willem De Vos

    Journal of Bacteriology   190 ( 23 )   7655 - 7665   2008年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    A two-component regulatory system of Lactobacillus plantarum, encoded by genes designated lamK and lamR (hpk10 and rrp10), was studied. The lamK and lamR genes encode proteins which are highly homologous to the quorum-sensing histidine kinase LamC and the response regulator LamA, respectively. Transcription analysis of the lamKR operon and the lamBDCA operon and liquid chromatography-mass spectrometry analysis of production of the LamD558 autoinducing peptide were performed for ΔlamA, ΔlamR, ΔlamA ΔlamR deletion mutants and a wild-type strain. The results suggested that lamA and lamR are cooperating genes. In addition, typical phenotypes of the ΔlamA mutant, such as reduced adherence to glass surfaces and filamentous cell morphology, were enhanced in the ΔlamA ΔlamR mutant. Microarray analysis suggested that the same cell wall polysaccharide synthesis genes, stress response-related genes, and cell wall protein-encoding genes were affected in the ΔlamA and ΔlamA ΔlamR mutants. However, the regulation ratio was more significant for the ΔlamA ΔlamR mutant, indicating the cooperative effect of LamA and LamR.

    DOI: 10.1128/JB.01489-07

  • Molecular monitoring of bacterial community structure in long-aged nukadoko: pickling bed of fermented rice bran dominated by slow-growing lactobacilli. 査読

    Jiro Nakayama, Hiroyuki Hoshiko, Mizuki Fukuda, Hidetoshi Tanaka, Naoshige Sakamoto, Shigemitsu Tanaka, Kazutoshi Ohue, Kenji Sakai, Kenji Sonomoto

    Journal of bioscience and bioengineering   104 ( 6 )   481 - 489   2007年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Nukadoko is the fermented rice bran bed traditionally used for pickling vegetables in Japan. Here, we investigate the bacterial community structure of nukadoko using several culture-independent methods. Denaturing gradient gel electrophoresis (DGGE) and sequence analysis of V2-V3 16S rRNA gene (16S rDNA) fragments amplified from a long-aged nukadoko bacterial community indicated seven predominant operational taxonomic units (OTUs) closely related to known Lactobacillus species. Phylogenetic analysis of these OTUs indicated a major cluster consisting of six OTUs including a dominant OTU closely related to Lactobacillus acidifarinae and one distinct OTU corresponding to Lactobacillus acetotolerans. L. acetotolerans was commonly detected as a dominant species in samples from different seasons. The succession of microbial community structure in the fermentation and ripening processes was investigated using a laboratory model nukadoko. The L. acidifarinae-like bacteria grew rapidly with a pH decrease in the first few days after inoculation, whereas L. acetotolerans grew slowly and became dominant after one week. Real-time quantitative polymerase chain reaction (Q-PCR) showed that the doubling time of L. acetotolerans was 12 h, while that of total bacteria was 4 h. Real-time quantitative reverse transcription polymerase chain reaction (Q-RT-PCR) targeting 16S rRNA showed a low metabolic activity of L. acetotolerans throughout the fermentation and ripening processes. Fluorescence in situ hybridization (FISH) showed that L. acetotolerans was a dominant bacterium in the ripening period and had a low metabolic activity. These results indicate that the slow-growing L. acetotolerans stably dominated nukadoko microbiota after the L. acidifarinae-like bacteria mainly contributed to the lactic acid fermentation of the rice bran.

    DOI: 10.1263/jbb.104.481

  • Differences in developing intestinal microbiota between allergic and non-allergic infants A pilot study in Japan 査読

    Prapa Songjinda, Jiro Nakayama, Atsushi Tateyama, Shigemitsu Tanaka, Mina Tsubouchi, Chikako Kiyohara, Taro Shirakawa, Kenji Sonomoto

    Bioscience, Biotechnology and Biochemistry   71 ( 9 )   2338 - 2342   2007年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    The bacterial compositions of feces were monitored in the first 2 months for 15 infants born in Japan, including eight subjects who developed allergy by the age of 2 years. Primer sets targeting six predominant bacterial groups in the infant intestine, Bacteroidaceae, Enterobacteriaceae, bifidobacteria, enterococci, lactobacilli, and the Clostridium perfringens group, were used for real-time PCR to quantitate each population in the feces. The population of Bacteroidaceae was significantly higher in the allergic group at the ages of 1 month (P = 0.03) and 2 months (P = 0.05) than in the non-allergic group, while no statistically significant difference was observed for the other bacterial populations.

    DOI: 10.1271/bbb.70154

  • Siamycin attenuates fsr quorum sensing mediated by a gelatinase biosynthesis-activating pheromone in Enterococcus faecalis. 査読 国際誌

    Jiro Nakayama, Emi Tanaka, Reiko Kariyama, Koji Nagata, Kenzo Nishiguchi, Ritsuko Mitsuhata, Yumi Uemura, Masaru Tanokura, Hiromi Kumon, Kenji Sonomoto

    Journal of bacteriology   189 ( 4 )   1358 - 1365   2007年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    The expression of two Enterococcus faecalis virulence-related proteases, gelatinase (GelE) and serine protease (SprE), is positively regulated by a quorum-sensing system encoded by the fsr gene cluster. In this system, E. faecalis secretes an autoinducing peptide, gelatinase biosynthesis-activating pheromone (GBAP), which triggers the FsrC-FsrA two-component regulatory system controlling the expression of two transcripts, fsrBDC and gelE-sprE. In the present study, we screened actinomycete metabolites for inhibitors of fsr quorum sensing. E. faecalis was cultured with each actinomycete culture supernatant tested, and the production of gelatinase and the production of GBAP were examined using the first screening and the second screening, respectively. Culture supernatant of Streptomyces sp. strain Y33-1 had the most potent inhibitory effect on both gelatinase production and GBAP production without inhibiting E. faecalis cell growth. The inhibitor in the culture supernatant was identified as a known peptide antibiotic, siamycin I. Siamycin I inhibited both gelatinase production and GBAP production at submicromolar concentrations, and it inhibited E. faecalis cell growth at concentrations above micromolar concentrations. Quantitative analysis of fsrBDC and gelE-sprE transcripts revealed that siamycin I suppressed the expression of both transcripts at a sublethal concentration. Siamycin I attenuated gelatinase production even when an overdose of GBAP was exogenously added to the culture. These results suggested that siamycin I inhibited the GBAP signaling via the FsrC-FsrA two-component regulatory system in a noncompetitive manner. The sublethal concentrations of siamycin I also attenuated biofilm formation. Treatment with siamycin could be a novel means of treating enterococcal infections.

    DOI: 10.1128/JB.00969-06

  • Revised model for Enterococcus faecalis fsr quorum-sensing system: the small open reading frame fsrD encodes the gelatinase biosynthesis-activating pheromone propeptide corresponding to staphylococcal agrd. 査読 国際誌

    Jiro Nakayama, Shengmin Chen, Nozomi Oyama, Kenzo Nishiguchi, Essam A Azab, Emi Tanaka, Reiko Kariyama, Kenji Sonomoto

    Journal of bacteriology   188 ( 23 )   8321 - 8326   2006年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Gelatinase biosynthesis-activating pheromone (GBAP) is an autoinducing peptide involved in Enterococcus faecalis fsr quorum sensing, and its 11-amino-acid sequence has been identified in the C-terminal region of the 242-residue deduced fsrB product (J. Nakayama et al., Mol. Microbiol. 41:145-154, 2001). In this study, however, we demonstrated the existence of fsrD, encoding the GBAP propeptide, which is in frame with fsrB but is translated independently of fsrB. It was also demonstrated that FsrB', an FsrD segment-truncated FsrB, functions as a cysteine protease-like processing enzyme to generate GBAP from FsrD. This revised model is consistent with the staphylococcal agr system.

    DOI: 10.1128/JB.00865-06

  • An agr-like two-component regulatory system in Lactobacillus plantarum is involved in production of a novel cyclic peptide and regulation of adherence. 国際誌

    Mark H J Sturme, Jiro Nakayama, Douwe Molenaar, Yoshiko Murakami, Ryoko Kunugi, Toshio Fujii, Elaine E Vaughan, Michiel Kleerebezem, Willem M de Vos

    Journal of bacteriology   187 ( 15 )   5224 - 5235   2005年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    We have analyzed a locus on the annotated Lactobacillus plantarum WCFS1 genome that showed homology to the staphylococcal agr quorum-sensing system and designated it lam for Lactobacillus agr-like module. Production of the lamBDCA transcript was shown to be growth phase dependent. Analysis of a response regulator-defective mutant (Delta)lamA) in an adherence assay showed that lam regulates adherence of L. plantarum to a glass surface. Global transcription analysis of the wild-type and (Delta)lamA strains in early, mid-, and late log phase of growth was performed using a clone-based microarray. Remarkably, only a small set of genes showed significant differences in transcription profiles between the wild-type and lamA mutant strains. The microarray analysis confirmed that lamBDCA is autoregulatory and showed that lamA is involved in regulation of expression of genes encoding surface polysaccharides, cell membrane proteins, and sugar utilization proteins. The lamBD genes encoding the putative autoinducing peptide precursor (LamD) and its processing protein (LamB) were overexpressed using the nisin-controlled expression system, and culture supernatants were analyzed by liquid chromatography/mass spectrometry (LC/MS) to identify overproduced LamD-derived peptides. In this way, a cyclic thiolactone pentapeptide that possesses a ring structure similar to those of autoinducing peptides of the staphylococcal agr system was identified. The peptide was designated LamD558, and its sequence (CVGIW) matched the annotated precursor peptide sequence. Time course analysis of wild-type culture supernatants by LC/MS indicated that LamD558 production was increased markedly from mid-log to late log growth phase. This is the first example of an agr-like system in nonpathogenic bacteria that encodes a cyclic thiolactone autoinducing peptide and is involved in regulation of adherence.

    DOI: 10.1128/JB.187.15.5224-5235.2005

  • Molecular monitoring of the developmental bacterial community in the gastrointestinal tract of Japanese infants 査読

    Prapa Songjinda, Jiro Nakayama, Yumiko Kuroki, Shigemitsu Tanaka, Sanae Fukuda, Chikako Kiyohara, Tetsuro Yamamoto, Kunio Izuchi, Taro Shirakawa, Kenji Sonomoto

    Bioscience, Biotechnology and Biochemistry   69 ( 3 )   638 - 641   2005年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    The dynamics of the developmental bacterial community in the Japanese neonatal gastrointestinal tract were examined by monitoring 16S ribosomal RNA gene (rDNA) diversity in fecal samples by PCR and denaturing gradient gel electrophoresis (DGGE). The results showed a certain pattern common in infants without antibiotic treatment, in which aerobes, e.g., Pseudomonas, appeared first and were then immediately replaced by facultative anaerobe, Enterococcus, Streptococcus, and Enterobacteriaceae through the first month, and finally strictly anaerobic Bifidobactrerium appeared.

    DOI: 10.1271/bbb.69.638

  • High-throughput PCR screening of genes for three-component regulatory system putatively involved in quorum sensing from low-G + C gram-positive bacteria. 国際誌

    Jiro Nakayama, Antoon D L Akkermans, Willem M De Vos

    Bioscience, biotechnology, and biochemistry   67 ( 3 )   480 - 489   2003年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Quorum sensing of gram-positive bacteria is often regulated by three-component regulatory system composed of autoinducing peptide, sensor kinase and response regulator. We used PCR to study a gene cassette encoding this three-component regulatory system. Degenerate primers were designed from consensus amino acid sequences in the HPK10 subfamily, mostly involved in quorum sensing. Products amplified from genomic DNA of Lactobacillus, Enterococcus, and Clostridium species were cloned and sequenced; their deduced amino acid sequences were similar to those of members of the HPK10 subfamily. Complete genes for the putative gene cassette were cloned by inverse PCR from L. paracasei E93490 and L. plantarum WCFS6. Phylogenetic analysis grouped the cloned putative HPKs into the HPK10 subfamily. These results indicated the usefulness of this high-throughput gene screening and suggested that the three-component regulatory gene cassette are widely present.

    DOI: 10.1271/bbb.67.480

  • Description of a 23.9-kilobase chromosomal deletion containing a region encoding fsr genes which mainly determines the gelatinase-negative phenotype of clinical isolates of Enterococcus faecalis in urine. 国際誌

    Jiro Nakayama, Reiko Kariyama, Hiromi Kumon

    Applied and environmental microbiology   68 ( 6 )   3152 - 3155   2002年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Expression of virulence-related extracellular proteases, gelatinase, and serine protease of Enterococcus faecalis is regulated by a quorum-sensing system encoded by the fsr gene cluster. In this study, a 23.9-kb chromosomal deletion containing the fsr gene cluster region was found to be present in the majority (79%) of gelatinase-negative clinical isolates of E. faecalis from urine.

    DOI: 10.1128/AEM.68.6.3152-3155.2002

  • Functional analysis of TraA, the sex pheromone receptor encoded by pPD1, in a promoter region essential for the mating response in Enterococcus faecalis 査読

    Takaaki Horii, Hiromichi Nagasawa, Jiro Nakayama

    Journal of Bacteriology   184 ( 22 )   6343 - 6350   2002年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Conjugative transfer of a bacteriocin plasmid, pPD1, of Enterococcus faecalis is induced in response to a peptide sex pheromone, cPD1, secreted from plasmid-free recipient cells. cPD1 is taken up by a pPD1 donor cell and binds to an intracellular receptor, TraA. Once a recipient cell acquires pPD1, it starts to produce an inhibitor of cPD1, termed iPD1, which functions as a TraA antagonist and blocks self-induction in donor cells. In this study, we discuss how TraA transduces the signal of cPD1 to the mating response. Gel mobility shift assays indicated that TraA is bound to a traA-ipd intergenic region, which is essential for cPD1 response. DNase I footprinting analysis suggested the presence of one strong (tab1) and two weak (tab2 and tab3) TraA-binding sites in the intergenic region. Primer extension analysis implied that the transcriptional initiation sites of traA and ipd were located in the intergenic region. Northern analysis showed that cPD1 upregulated and downregulated transcription of ipd and traA, respectively. The circular permutation assay showed that TraA bent a DNA fragment corresponding to the tab1 region, and its angle was changed in the presence of cPD1 or iPD1. From these data, we propose a model that TraA changes the conformation of the tab1 region in response to cPD1 and upregulates the transcription of ipd, which may lead to expression of genes required for the mating response.

    DOI: 10.1128/JB.184.22.6343-6350.2002

  • Chemical synthesis and biological activity of the gelatinase biosynthesis-activating pheromone of enterococcus faecalis and its analogs

    Jiro Nakayama, Yong Cao, Takaaki Horii, Shohei Sakuda, Hiromichi Nagasawa

    Bioscience, Biotechnology and Biochemistry   65 ( 10 )   2322 - 2325   2001年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    An 11-residue peptide lactone, termed the gelatinase biosynthesis-activating pheromone (GBAP), triggers the production of the pathogenicit)-related extracellular proteases, gelatinase and serine protease, in Enterococcus faecalis. In this study, we synthesized GBAP and its analogs and examined their gelatinase biosynthesis-inducing activity. This study on the structure-activity relationship shows that a lactone ring was indispensable for the activity.

    DOI: 10.1271/bbb.65.2322

  • Gelatinase biosynthesis-activating pheromone: a peptide lactone that mediates a quorum sensing in Enterococcus faecalis 査読

    Jiro Nakayama, Yong Cao, Takaaki Horii, Shohei Sakuda, Antoon D L Akkermans, Willem M. De Vos, Hiromichi Nagasawa

    Molecular Microbiology   41 ( 1 )   145 - 154   2001年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Biosynthesis of gelatinase, a virulence factor of Enterococcus faecalis, was found to be regulated in a cell density-dependent fashion in which its production is active in late log to early stationary phase. Addition of early stationary phase culture filtrate to medium shifted the onset of gelatinase production to that of mid-log phase, suggesting that E. faecalis secretes a gelatinase biosynthesis-activating pheromone (GBAP). GBAP was isolated from culture supernatant of E. faecalis OG1S-P. Structural analysis suggested GBAP to be an 11-residue cyclic peptide containing a lactone structure, in which the α-carboxyl group of the C-terminal amino acid is linked to a hydroxyl group of the serine of the third residue. A synthetic peptide possessing the deduced structure showed GBAP activity at nanomolar concentrations as did natural GBAP. Database searches revealed that GBAP corresponds to a C-terminal part of a 242-residue FsrB protein. Northern analysis showed that GBAP slowly induces the transcription of two operons, fsrB-fsrC encoding FsrB and a putative histidine kinase FsrC and gelE-sprE encoding gelatinase GelE and serine protease SprE. Strains with an insertion mutation in either fsrC or a putative response regulator gene fsrA failed to respond to GBAP, suggesting that the GBAP signal is transduced by a two-component regulatory system.

    DOI: 10.1046/j.1365-2958.2001.02486.x

  • Analysis of pheromone binding and pheromone reception by Enterococcus faecalis 査読

    Jiro Nakayama, Yuuichiro Takanami, Akinori Suzuki

    Advances in Experimental Medicine and Biology   418   1033 - 1035   1997年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • Targeted disruption of the PD78 gene (traF) reduces pheromone-inducible conjugal transfer of the bacteriocin plasmid pPD1 in Enterococcus faecalis 査読

    Jiro Nakayama, Don B. Clewell, Akinori Suzuki

    FEMS Microbiology Letters   128 ( 3 )   283 - 288   1995年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Bacterial sex pheromone, cPD1, induces sexual aggregation of Enterococcus faecalis harboring the bacteriocin plasmid, pPD1, and enables pPD1 to transfer at high frequency in a liquid culture. PD78 is a cPD1-inducible cell surface protein encoded by pPD1. The PD78 gene, traF, was disrupted by homologous recombination between pPD1 and an artificial vector having a deletion in the middle portion of traF. The disruption of traF did not affect the cPD1-inducible aggregation but reduced the transfer frequency of pPD1 to 2% of the wild-type level.

    DOI: 10.1016/0378-1097(95)00118-O

  • Cloning and characterization of a region of Enterococcus faecalis plasmid pPD1 encoding pheromone inhibitor (ipd), pheromone sensitivity (traC), and pheromone shutdown (traB) genes 査読

    Jiro Nakayama, K. Yoshida, H. Kobayashi, A. Isogai, D. B. Clewell, A. Suzuki

    Journal of Bacteriology   177 ( 19 )   5567 - 5573   1995年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Bacteriocin plasmid pPD1 in Enterococcus faecalis encodes a mating response to recipient-produced sex pheromone cPD1. Once a recipient acquires pPD1, transconjugants apparently shut off cPD1 activity in broth culture and no longer behave as recipients for pPD1. This event is performed by synthesis of the pheromone inhibitor iPD1 and also by repression of cPD1 production, the so-called 'pheromone shutdown.' A 5.4-kb EcoRV-HincII segment of pPD1, which expressed iPD1 in Escherichia coli, was sequenced and found to be organized as traC-traB-traA-ipd; each open reading frame is analogous to that found in other pheromone plasmids, pAD1 and pCF10, and thus is designated in accordance with the nomenclature in pAD1. The ipd gene encodes a peptide consisting of 21 amino acids, in which the C-terminal eight residues correspond to iPD1. The putative TraC product has a strong similarity to oligopeptide-binding proteins found in other bacterial species, as do pheromone-binding proteins of pCF10 and pPD1. A strain carrying traC- disrupted pPD1 required a concentration of cPD1 fourfold higher than that needed by the wild-type strain for induction of sexual aggregation. These results suggest that the TraC product contributes to pheromone sensitivity as o pheromone-binding protein. A strain transformed with traB-disrupted pPD1 produced a high level of cPD1 similar to that produced by plasmid-free recipients and underwent self-induction. Thus, the TraB product contributes to cPD1 shutdown.

    DOI: 10.1128/jb.177.19.5567-5573.1995

  • Isolation and Structure of the Sex Pheromone Inhibitor, iAM373, of Enterococcus faecalis 査読

    Jiro Nakayama, Yukitsugu Ono, Akinori Suzuki

    Bioscience, Biotechnology and Biochemistry   59 ( 7 )   1358 - 1359   1995年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    An Enterococcus faecalis plasmid, pAM373, has a high frequency of transfer in a liquid medium when induced by a recipient-produced sex pheromone, cAM373. The sex pheromone inhibitor against cAM373, termed iAM373, was isolated from a culture supernatant of E. faecalis harboring pAM377 (=pAM373::Tn917), and its structure was identified as a heptapeptide, H-Ser-Ile-Phe-Thr-Leu-Val-Ala-OH.

    DOI: 10.1271/bbb.59.1358

  • Isolation and Structure of the Enterococcus faecalis Sex Pheromone, cOB1, that Induces Conjugal Transfer of the Hemolysin/Bacteriocin Plasmids, pOB1 and pYI1 査読

    Jiro Nakayama, Yuki Abe, Yukitsugu Ono, Akira Isogai, Akinori Suzuki

    Bioscience, Biotechnology and Biochemistry   59 ( 4 )   703 - 705   1995年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    A bacterial sex pheromone, cOB1, which induces conjugal transfer of the Enterococcus faecalis hemolysin-bacteriocin (Hly/Bac) plasmid, pOB1, was isolated from the culture broth of pOB1-free E. faecalis. Its structure was found to be a hydrophobic octapeptide, H-Val-Ala-Val-Leu-Val-Leu-Gly-Ala-OH. The cOB1 peptide induced the mating response of not only pOB1 but also another incompatibility group Hly/Bac plasmid, pYI1.

    DOI: 10.1271/bbb.59.703

  • The prgQ gene of the Enterococcus faecalis tetracycline resistance plasmid pCF10 encodes a peptide inhibitor, iCF10 査読

    Jiro Nakayama, R. E. Ruhfel, G. M. Dunny, A. Isogai, A. Suzuki

    Journal of Bacteriology   176 ( 23 )   7405 - 7408   1994年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Conjugative transfer of the Enterococcus faecalis tetracycline resistance plasmid pCF10 is stimulated by a peptide pheromone, cCF10. Once a recipient strain acquires pCF10 and thus becomes a pheromone-responsive donor, cCF10 activity is no longer detected in culture filtrates. Here we show that pCF10 encodes a peptide inhibitor, iCF10, secreted by donor cells; this inhibitor antagonizes the cCF10 activity in culture filtrates. In order to detect and quantitate iCF10, we developed a reverse-phase high-performance liquid chromatography assay in which the inhibitor peptide elutes separately from the pheromone; this type of assay enabled us to determine that lack of pheromone activity in donor culture filtrates was due to secretion of a mixture of iCF10 and cCF10, rather than abolition of cCF10 secretion. The gene encoding iCF10, prgQ, is located on the EcoRI-C fragment of pCF10. The open reading frame comprising the prgQ gene encodes a 23-amino-acid precursor that resembles a signal peptide. This precursor is cleaved to the mature heptapeptide iCF10 during the secretion process.

    DOI: 10.1128/jb.176.23.7405-7408.1994

  • C-Terminal Identification of AD74, a Proteolytic Product of Enterococcus faecalis Aggregation Substance Application of Liquid Chromatography/Mass Spectrometry 査読

    Jiro Nakayama, Hiroshi Watarai, Akira Isogai, Akinori Suzuki

    Bioscience, Biotechnology and Biochemistry   56 ( 1 )   127 - 131   1992年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Sexual aggregation involved in conjugative transfer of Enterococcus faecalis plasmid pADl is enhanced by the sex pheromone cADl, which is excreted from recipient cells. A membrane-anchored 137 kDa protein is a pADl-encoded aggregation substance designated asal, which is responsible for cell-cell contact and leads to the aggregation of cells. An AD74 protein is a proteolytic product corresponding to the N-terminal half of asal. The C-terminal of AD74 was identified as lysine at position 510 (K-510) by liquid chromatography/mass spectrometry (LC/MS): it indicates that asal is cleaved specifically between K-510 and G-511.

    DOI: 10.1271/bbb.56.127

  • Immunological Characterization of Pheromone-induced Proteins Associated with Sexual Aggregation in Enterococcus faecalis 査読

    Jiro Nakayama, Hiroshi Watarai, Akira Isogai, Akinori Suzuki

    Bioscience, Biotechnology and Biochemistry   56 ( 2 )   264 - 269   1992年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Sexual aggregation involved in conjugative transfer of the Enterococcus faecalis plasmids pPDl and pADl is enhanced by sex pheromones cPDl and cADl, respectively, which are excreted from recipient cells. PD78 (78kDa) and AD74 (74kDa) were detectable on the surface of donors harboring pPDl and pAD1, respectively, at the time of cell aggregation. The proteins PD78 and AD74 were purified and used to raise anti-PD78 and anti-AD74 antibodies. The antibodies blocked the sexual aggregation and the plasmid transfer. Anti-AD74 antibody reacted to both 153 kDa proteins extracted from cPDl and cADl-induced donor cells after lysozyme digestion of cell wall. Pheromone-induced synthesis of PD78 and AD74, when both plasmids were present in the same cell, was independent.

    DOI: 10.1271/bbb.56.264

  • Nucleotide sequence of the sex pheromone inhibitor (iAD1) determinant ofEnterococcus faecalis conjugative plasmid pAD1 査読

    Don B. Clewell, Linda T. Pontius, Florence Y. An, Yasuyoshi Ike, Akinori Suzuki, Jiro Nakayama

    Plasmid   24 ( 2 )   156 - 161   1990年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    The determinant for the peptide sex pheromone inhibitor iAD1 (iad) on the hemolysin/bacteriocin plasmid pAD1 ofEnterococcus faecalis was sequenced. The sequence reveals a 22-amino-acid precursor with the car{ballot box}yl-terminal 8 residues corresponding to iAD1. It appears that iAD1 is a component of its own signal sequence. © 1990 Academic Press, Inc. All rights reserved.

    DOI: 10.1016/0147-619X(90)90019-9

  • Efficient extracellular expression of a foreign protein in smptomyos using secretory protease inhibitor (SSI) gene fusions 査読

    Seiichi Taguchit, Izumi Kumagai, Jiro Nakayama, Akinori Suzuki, Kin Ichiro Miura

    Nature Biotechnology   7 ( 10 )   1063 - 1066   1989年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Using the gene for a secreted protease inhibitor, Streptomyces subtilisin inhibitor (SSI), we have established a secretory expression system for the products of foreign genes in Streptomyces. A sex pheromone peptide, cADl, of Enterococcus fae- calis was expressed and secreted as a stable complete fusion protein with SSI in amounts comparable to the native SSI in S. lividans 66. Recombinant cADl, isolated from the fusion protein by chemical digestion with BrCN, has the same amino acid composition and sequence, retention time on reverse phase HPLC, molecular mass, and biological activity as authentic cADl. This system should be useful for the efficient expression of other foreign gene products, and as a model for heterologous gene expression in Streptomyces. © 1989 Nature Publishing Group.

    DOI: 10.1038/nbt1089-1063

  • Isolation and Structural Elucidation of a New Cyclitol Derivative, Streptol, as a Plant Growth Regulator 査読

    Akira Isogai, Shouhei Sakuda, Jiro Nakayama, Satoshi Watanabe, Akinori Suzuki

    Bioscience, Biotechnology and Biochemistry   51 ( 8 )   2277 - 2279   1987年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    A new cyclitol derivative, streptol (1), was isolated from a culture filtrate of an unidentified Streptomyces sp., and the structure of 1 was determined as 1d-(1,2,4/3)-5- hydroxymethyl-5-cyclohexene-l,2,3,4-tetrol. This structure was elucidated with NMR and MS spectrometry, and the absolute configuration was determined from the CD spectrum of the tetrabenzoate of the hydrogenolized compound of 1. Streptol inhibited the root growth of lettuce seedlings at a concentration above 13 ppm.

    DOI: 10.1271/bbb1961.51.2277

  • Probiotic Chocolate Containing <i>Lactobacillus plantarum</i> Dad-13 Alters the Gut Microbiota Composition of Undernourished Children in Lombok: A Randomized Double-Blind Trial

    Rahayu, ES; Yoga, WK; Komalasari, H; Mariyatun, M; Yuda, WA; Manurung, NEP; Hasan, PN; Suharman, S; Pamungkaningtyas, FH; Nurfiana, DA; Pramesi, PC; Gatya, M; Therdtatha, P; Nakayama, J; Juffrie, M; Djaafar, TF; Marwati, T; Utami, T

    INTERNATIONAL JOURNAL OF FOOD SCIENCE   2024   9493797   2024年8月   ISSN:2356-7015 eISSN:2314-5765

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    記述言語:英語   出版者・発行元:International Journal of Food Science  

    The present study investigated the ingestion effect of chocolate probiotic containing Lactobacillus plantarum Dad-13 in undernourished children. A 100-day observation was conducted on undernourished children in Lombok, who were divided into probiotic (n=28) and placebo (n=28) groups. Fecal sampling was performed on the 10th and 100th days and further analyzed for gut microbiota composition, short-chain fatty acid (SCFA), and fecal pH. A significant difference was found in the diversity index, fecal pH, and several microbiotas at the phylum and genus levels. At the phylum level, Bacteroidetes was significantly higher in the probiotic group, and a higher relative abundance (RA) of Firmicutes was found in the placebo group. At the genus level, significant differences were observed in some bacteria, such as Bifidobacterium and Prevotella. Therefore, it can be concluded that the probiotic intervention in this study resulted in changes of gut microbiota diversity and fecal pH.Trial Registration: Thai Clinical Trials Registry identifier: TCTR20220425001.

    DOI: 10.1155/2024/9493797

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  • Gut microbiome signatures associated with type 2 diabetes in obesity in Mongolia

    Shinoda, A; Lkhagvajav, T; Mishima, R; Therdtatha, P; Jamiyan, D; Purevdorj, C; Sonomtseren, S; Chimeddorj, B; Namdag, B; Lee, YK; Demberel, S; Nakayama, J

    FRONTIERS IN MICROBIOLOGY   15   1355396   2024年6月   ISSN:1664-302X eISSN:1664-302X

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    記述言語:英語   出版者・発行元:Frontiers in Microbiology  

    Mongolian people possess a unique dietary habit characterized by high consumption of meat and dairy products and fewer vegetables, resulting in the highest obesity rate in East Asia. Although obesity is a known cause of type 2 diabetes (T2D), the T2D rate is moderate in this population; this is known as the “Mongolian paradox.” Since the gut microbiota plays a key role in energy and metabolic homeostasis as an interface between food and body, we investigated gut microbial factors involved in the prevention of the co-occurrence of T2D with obesity in Mongolians. We compared the gut microbiome and metabolome of Mongolian adults with obesity with T2D (DO: n = 31) or without T2D (NDO: n = 35). Dysbiotic signatures were found in the gut microbiome of the DO group; lower levels of Faecalibacterium and Anaerostipes which are known as short-chain fatty acid (SCFA) producers and higher levels of Methanobrevibacter, Desulfovibrio, and Solobacterium which are known to be associated with certain diseases. On the other hand, the NDO group exhibited a higher level of fecal SCFA concentration, particularly acetate. This is consistent with the results of the whole shotgun metagenomic analysis, which revealed a higher relative abundance of SCFA biosynthesis-related genes encoded largely by Anaerostipes hadrus in the NDO group. Multiple logistic regression analysis including host demographic parameters indicated that acetate had the highest negative contribution to the onset of T2D. These findings suggest that SCFAs produced by the gut microbial community participate in preventing the development of T2D in obesity in Mongolians.

    DOI: 10.3389/fmicb.2024.1355396

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  • アカラシアにおけるTh17免疫反応と関連した食道細菌叢の変化とLC20不全リン酸化の相互作用(The interplay between alterations in esophageal microbiota associated with Th17 immune response and impaired LC20 phosphorylation in achalasia)

    Ikeda Hiroko, Ihara Eikichi, Takeya Kosuke, Mukai Koji, Onimaru Manabu, Ouchida Kenoki, Hata Yoshitaka, Bai Xiaopeng, Tanaka Yoshimasa, Sasaki Taisuke, Saito Fumiyo, Eto Masumi, Nakayama Jiro, Oda Yoshinao, Nakamura Masafumi, Inoue Haruhiro, Ogawa Yoshihiro

    Journal of Gastroenterology   59 ( 5 )   361 - 375   2024年5月   ISSN:0944-1174

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    記述言語:英語   出版者・発行元:シュプリンガー・ジャパン(株)  

    食道平滑筋収縮の変化および関連する炎症性反応について検討することによりアカラシアの病態を明らかにし、アカラシアの進展における食道細菌叢の意義を評価した。経口的内視鏡的筋層切開術を施行したII型アカラシア症例から得た食道粘膜および下部食道括約筋(LES)標本を解析した。患者由来の食道調整培養液をマウス食道に投与し、食道内環境がアカラシアと関連するか否かについて検討した。安静時および刺激時における対照群のLESでは、20-kDaミオシン軽鎖(LC20)の約30%がリン酸化されていた。アカラシア症例では状況に関わらずLC20のリン酸化は10%未満であった。アカラシアにおける低リン酸化は、ミオシンリン酸化阻害蛋白質CPI-17の発現低下と関連していた。IL-17A、IL-17F、IL-22、IL-23Aを含む関連サイトカインは、アカラシアでは発現が増加していた。食道細菌叢のα-Diversity指数、およびActinomycesおよびDialisterを含む数種類の細菌の割合がアカラシアで増加していた。ActinomycesレベルはIL-23Aレベルと正の相関が認められ、DialisterレベルはIL-17A、IL-17F、IL-22レベルと正の相関が認められた。マウスにおいて、食道IL-17F値は調整培地の経口投与後に増加した。

  • The interplay between alterations in esophageal microbiota associated with Th17 immune response and impaired LC20 phosphorylation in achalasia

    Ikeda, H; Ihara, E; Takeya, K; Mukai, K; Onimaru, M; Ouchida, K; Hata, Y; Bai, XP; Tanaka, Y; Sasaki, T; Saito, F; Eto, M; Nakayama, J; Oda, Y; Nakamura, M; Inoue, H; Ogawa, Y

    JOURNAL OF GASTROENTEROLOGY   59 ( 5 )   361 - 375   2024年3月   ISSN:0944-1174 eISSN:1435-5922

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Journal of Gastroenterology  

    BACKGROUND: Achalasia is an esophageal motility disorder with an unknown etiology. We aimed to determine the pathogenesis of achalasia by studying alterations in esophageal smooth muscle contraction and the associated inflammatory response, and evaluate the role of esophageal microbiota in achalasia development. METHODS: We analyzed esophageal mucosa and lower esophageal sphincter (LES) samples, obtained from patients with type II achalasia who underwent peroral endoscopic myotomy. Esophageal conditioned media obtained from patients were transferred into the mouse esophagus to determine whether the esophageal intraluminal environment is associated with achalasia. RESULTS: Approximately 30% of 20-kDa myosin light chains (LC20) was phosphorylated in LES from the control group under resting and stimulated conditions, whereas less than 10% of LC20 phosphorylation was detected in achalasia under all conditions. The hypophosphorylation of LC20 in achalasia was associated with the downregulation of the myosin phosphatase-inhibitor protein CPI-17. Th17-related cytokines, including IL-17A, IL-17F, IL-22, and IL-23A, were significantly upregulated in achalasia. α-Diversity index of esophageal microbiota and the proportion of several microbes, including Actinomyces and Dialister, increased in achalasia. Actinomyces levels positively correlated with IL-23A levels, whereas Dialister levels were positively associated with IL-17A, IL-17F, and IL-22 levels. Esophageal IL-17F levels increased in mice after oral administration of the conditioned media. CONCLUSIONS: In LES of patients with achalasia, hypophosphorylation of LC20, a possible cause of impaired contractility, was associated with CPI-17 downregulation and an increased Th17-related immune response. The esophageal intraluminal environment, represented by the esophageal microbiota, could be associated with the development and exacerbation of achalasia.

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  • Ziziphus spina-christi (L.) Willd. Leaves Extract Affecting agr Quorum Sensing System in Staphylococcus aureus

    Refai, MY; Elazzazy, AM; Abu-Elghait, M; Desouky, S; Albureikan, MOI; Nakayama, J

    ARABIAN JOURNAL FOR SCIENCE AND ENGINEERING   49 ( 1 )   97 - 105   2024年1月   ISSN:2193-567X eISSN:2191-4281

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    出版者・発行元:Arabian Journal for Science and Engineering  

    Ziziphus spina-christi L. (Sider) is a longstanding Arabian medicinal herb. The present study aimed to explore whether Ziziphus spina-christi leaves extract (ZSCLE) affects agr quorum sensing (QS) system and the phenotypic blood hemolytic activity regulated by this system in Staphylococcus aureus. The luciferase is encoded by plasmid pSB2035 in the reporter strain 8325–4, which is promoted by the agr P3 promoter. The result indicated that the total extract of (ZSCL) is effective against agr QS system in the reporter strain, where it reduces the lux gene expression up to 80.41% at a concentration of 0.25 mg/mL of total extract. Also, the hemolytic activity was reduced up to 98.9% by comparing with positive control by total extract. Successive extracts: hexane, diethyl ether, chloroform, ethyl acetate, and methanol were performed. The result revealed that the diethyl ether fraction was the greatest one that inhibited the production of both agr locus and the phenotypic hemolysin protein of S. aureus. Accordingly, phytochemical analysis in this total extract was performed and showed a high number of flavonoids and other compounds. FT-IR analysis of the most active fraction showed Monoelaidin as the main component of this fraction.

    DOI: 10.1007/s13369-023-08404-5

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  • Impact of vegetables on the microbiota of the rice bran pickling bed Nukadoko

    SUGIURA Shunsaku, IKEDA Mika, NAKAMURA Yuichi, MISHIMA Riko, MORISHITA Mika, NAKAYAMA Jiro

    Bioscience of Microbiota, Food and Health   advpub ( 0 )   2024年   eISSN:21863342

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    記述言語:英語   出版者・発行元:BMFH出版会  

    <p>Nukadoko, a fermented rice bran bed for pickling vegetables called nukazuke, has a complex microbiota. Within it, deep interactions between the microbiota of the pickled vegetables and nukadoko characterize and control the qualities of both products. To address this notion, we monitored the changes in the microbiota of nukadoko and nukazuke while pickling different vegetables. Raw or roasted rice bran was mixed with salted water and fermented at 24℃ for 40 days, following which different species of vegetable, <i>Cucumis sativus</i> var. <i>sativus</i>, <i>Brassica oleracea</i> var. <i>capitata</i>, or <i>Raphanus sativus</i> var. <i>hortensis</i>, were pickled. The microbial composition of the washing solution of fresh vegetables, as well as that of the nukadoko and nukazuke for each vegetable, was analyzed by amplicon sequencing of 16S rRNA genes. Although the microbiota of nukadoko varied depending on the species of pickled vegetables, no transcolonization of any species of bacteria from fresh vegetables to nukadoko was observed. However, some lactic acid bacterium (LAB) species eventually dominated the microbiota of both nukazuke and matured nukadoko, although they were not detected in either the fresh vegetables or rice bran. Particularly, <i>Lactiplantibacillus plantarum </i>was dominant among all pairs of pickled vegetables and matured nukadoko, whereas the transcolonization of some other LAB species was observed in a pickled vegetable-specific manner. <i>Staphylococcus xylosus</i> was observed to some extent in each nukadoko, yet it was not detected in any nukazuke. Overall, a LAB-dominant microbiota was established in both nukadoko and nukazuke in an underlying process that was different but partly common among vegetables.</p>

    DOI: 10.12938/bmfh.2023-104

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  • Effect of lactic acid bacterial starter KUB-G2 on grass silage quality and its microbial community performed using 140-ton plastic bag silos: a large-scale study

    Therdtatha, P; La-Ongkham, O; Nakphaichit, M; Mapato, C; Rungruang, S; Nakayama, J; Nitisinprasert, S

    QUALITY ASSURANCE AND SAFETY OF CROPS & FOODS   15   1 - 13   2024年   ISSN:1757-8361 eISSN:1757-837X

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    出版者・発行元:Quality Assurance and Safety of Crops and Foods  

    This study investigated the effectiveness of lactic acid bacteria (LAB) starters in 140 tons of signal grass silage. We compared a locally developed starter, a combination of Lactiplantibacillus plantarum KUB-SP1-3 and Pediococcus acidilactici KUB-M6 (KUB-G2) with a commercial starter. We monitored the chemical and microbiological properties of silage at ensiling (day 0) and after 21 days. The results indicated that KUB-G2 produced high-quality silage. There were no significant differences in total viable microbes, lactic acid, or propionic acid contents between the starters. However, the silage produced with KUB-G2 had a lower acetic acid content than that produced with the commercial starter. KUB-G2 also resulted in a narrower microbiome profile dominated by Pediococci whereas the control group displayed a broader range of bacterial taxa with colonization by Lactiplantibacillus and Lentilactobacillus. These findings suggested that KUB-G2, with its distinct microbial profile, is a suitable replacement for imported commercial starters in large-scale signal grass silage production.

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  • Cyclic pairwise interaction representing a rock–paper–scissors game maintains the population of the vulnerable yeast Saccharomyces cerevisiae within a multispecies sourdough microbiome

    大城 麦人, 善藤 威史, 田代 幸寛, 中山 二郎

    Microbiology Spectrum   11 ( 6 )   e01370-23   2023年12月   eISSN:21650497

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    記述言語:英語   出版者・発行元:American Society for Microbiology  

    Sourdough starters are spontaneously generated multispecies communities consisting of lactic acid bacteria (LAB) and yeasts. Traditionally used to make baked goods, these communities of microorganisms can be propagated through successive transfers to other containers. Successive transfers result in microbial community dynamics; however, the mechanisms guiding the dynamics are not fully elucidated. This study tracked five species of sourdough LAB-yeast communities (three LAB species and two yeast species) during successive transfers in vitro to explore the interspecies interactions driving the multispecies community dynamics. The microbial dynamics were modeled with a generalized Lotka–Volterra (gLV) equation, which can mathematically identify ecological types of interspecies interactions. The gLV model simulated the population dynamics of each species with Pearson’s correlation coefficient values of ≥0.817. Pairwise experiments revealed that competition (negative/negative) predominated among all pairwise interactions (70%), followed by amensalism (negative/neutral) (30%). In the pairwise community, the LAB species Limosilactobacillus fermentum suppressed Saccharomyces cerevisiae growth through amensalism; however, S. cerevisiae population was maintained when the community comprised 3–5 species, including the LAB species L. fermentum and Pediococcus pentosaceus. The key interspecies interactions for maintaining the S. cerevisiae population were theoretically determined using the gLV model; the three identified species interacted non-transitively with S. cerevisiae in a cyclic pairwise interaction, metaphorically representing a rock–paper–scissors relationship, which systemically supported the S. cerevisiae population in the multispecies community. These theoretical insights indicate that cyclic pairwise interaction is the main driver of LAB-yeast population dynamics, which helps sustain a vulnerable yeast species in a sourdough multispecies community.

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  • Cyclic pairwise interaction representing a rock-paper-scissors game maintains the population of the vulnerable yeast <i>Saccharomyces cerevisiae</i> within a multispecies sourdough microbiome 国際誌

    Oshiro, M; Zendo, T; Tashiro, Y; Nakayama, J

    MICROBIOLOGY SPECTRUM   11 ( 6 )   e0137023   2023年12月   ISSN:2165-0497

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Microbiology Spectrum  

    Traditionally, multispecies consisting of lactic acid bacteria and yeasts collaboratively engage sourdough fermentation, which determines the quality of the resulting baked goods. Nonetheless, the successive transfer of these microbial communities can result in undesirable community dynamics that prevent the formation of high-quality sourdough bread. Thus, a mechanistic understanding of the community dynamics is fundamental to engineer sourdough complex fermentation. This study describes the population dynamics of five species of lactic acid bacteria-yeast communities in vitro using a generalized Lotka-Volterra model that examines interspecies interactions. A vulnerable yeast species was maintained within up to five species community dynamics by obtaining support with a cyclic interspecies interaction. Metaphorically, it involves a rock-paper-scissors game between two lactic acid bacteria species. Application of the generalized Lotka-Volterra model to real food microbiomes including sourdoughs will increase the reliability of the model prediction and help identify key microbial interactions that drive microbiome dynamics.

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  • Interception of Epoxide ring to quorum sensing system in Enterococcus faecalis and Staphylococcus aureus 国際誌

    Refai, MY; Elazzazy, AM; Desouky, SE; Abu-Elghait, M; Fayed, EA; Alajel, SM; Alajlan, AA; Albureikan, MO; Nakayama, J

    AMB EXPRESS   13 ( 1 )   126 - 126   2023年11月   ISSN:2191-0855

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMB Express  

    Quorum sensing inhibitor (QSI) has been attracting attention as anti-virulence agent which disarms pathogens of their virulence rather than killing them. QSI marking cyclic peptide-mediated QS in Gram-positive bacteria is an effective tool to overcome the crisis of antibiotic-dependent chemotherapy due to the emergence of drug resistance strain, e.g., methicillin resistant Staphylococcus aureus (MRSA) and Vancomycin resistant Enterococci (VRE). From a semi-large-scale screening thus far carried out, two Epoxide compounds, Ambuic acid and Synerazol, have been found to efficiently block agr and fsr QS systems, suggesting that the Epoxide group is involved in the mode of action of these QSIs. To address this notion, known natural Epoxide compounds, Cerulenin and Fosfomycin were examined for QSI activity for the agr and fsr systems in addition to in silico and SAR studies. As a result, most of investigated Epoxide containing antibiotics correlatively interfere with QSI activity for the agr and fsr systems under sublethal concentrations.

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  • Interaction of diet and gut microbiome in Asians and its effect on their health

    Nakayama, J; Mishima, R; Shinoda, A; Rahayu, ES; Nakphaichit, M; Demberel, S; Dalmacio, LM; Lee, YK

    ANNALS OF NUTRITION AND METABOLISM   79   135 - 135   2023年8月   ISSN:0250-6807 eISSN:1421-9697

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  • Longitudinal gut mycobiota changes in Japanese infants during first three years of life

    Mishima, R; Tanaka, M; Momoda, R; Sanefuji, M; Morokuma, S; Ogawa, M; Kato, K; Nakayama, J

    JOURNAL OF BIOSCIENCE AND BIOENGINEERING   135 ( 4 )   266 - 273   2023年4月   ISSN:1389-1723 eISSN:1347-4421

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Journal of Bioscience and Bioengineering  

    Although fungi can have a large impact on host health through the stimulation of the immune system and toxin production, few studies have investigated the gut mycobiota during infancy, a period during which sensitivity to internal and external stimuli is high. To capture the trend in fungal colonization during infancy, we evaluated the gut mycobiota of ten Japanese infants during the first 3 years of life. Infants had two major phyla, Ascomycota (68.9%) and Basidiomycota (29.6%), and the most abundant genus was Saccharomyces (26.8%), followed by Malassezia (18.5%), Candida (12.3%), Meyerozyma (8.5%), and Penicillium (8.3%). Alpha diversity analysis revealed a significant decrease in fungal richness and evenness with age, suggesting adaptive selection of the colonizing species in the gut environment. Beta diversity analysis divided infant mycobiota into age-related clusters and showed discrete separation before and after weaning, suggesting shift in microenvironment via weaning. In the initial stage, a variety of fungal species that likely originated from an environment, such as Malassezia spp., was highly colonized and were replaced by yeasts, such as Saccharomyces, after weaning. Further studies are needed to shed light on how the passage of the series of fungal colonizations in infancy affects the development of the host immune system and the other homeostasis involved in health later in life.

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  • 生後3年間の日本人乳児における長期的腸内真菌叢の変化(Longitudinal gut mycobiota changes in Japanese infants during first three years of life)

    Mishima Riko, Tanaka Masaru, Momoda Rie, Sanefuji Masafumi, Morokuma Seiichi, Ogawa Masanobu, Kato Kiyoko, Nakayama Jiro

    Journal of Bioscience and Bioengineering   135 ( 4 )   266 - 273   2023年4月   ISSN:1389-1723

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    記述言語:英語   出版者・発行元:(公社)日本生物工学会  

    乳児期の真菌定着の傾向を検索するため、生後3年間の腸内真菌叢を分析した。その結果、子嚢菌門(68.9%)と担子菌門(29.6%)が主要な門で、最も豊富な属はSaccharomyces(26.8%)、続いてMalassezia(18.5%)、Candida(12.3%)、Meyerozyma(8.5%)、Penicillium(8.3%)であった。α多様性分析では、年齢とともに真菌の多様性と均一性が減少したことから、腸環境における定着種の適応選択が示唆された。β多様性分析では、乳児真菌叢は年齢関連クラスターに分類され、離乳前後で個別的分離を示したことから、離乳を介して微小環境が変化したことが示唆された。以上より、最初の段階ではMalassezia種のような環境からと考えられる真菌種が多く定着し、離乳後にはSaccharomycesのような酵母に置き換わると考えられた。

  • 修士学生に就活フリーの時間を

    中山 二郎

    化学と生物   61 ( 3 )   103 - 103   2023年3月   ISSN:0453073X eISSN:18836852

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    記述言語:日本語   出版者・発行元:公益社団法人 日本農芸化学会  

    DOI: 10.1271/kagakutoseibutsu.61.103

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  • 変わりゆくアジア食とアジア人の腸内細菌叢

    中山 二郎

    Microbiome Science   2   6 - 12   2023年

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  • 各種胆汁酸分子の発芽誘導能を用いた難培養性腸内細菌の分離と日本人腸内細菌叢カクテルの創製

    中山 二郎

    発酵研究所助成研究報告集   37 ( 0 )   128   2023年   ISSN:00738751 eISSN:27592553

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    記述言語:日本語   出版者・発行元:公益財団法人 発酵研究所  

    DOI: 10.60396/iforc.37.0_128

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  • DYSBIOSIS IN CHRONIC HEPATITIS C WORSENS WITH AGING

    Inoue, T; Nakayama, J; Moriya, K; Kawaratani, H; Iio, E; Matsuura, K; Watanabe, T; Nagaoka, K; Tateyama, M; Setoyama, H; Yoshimaru, Y; Yoshiji, H; Tanaka, Y

    HEPATOLOGY   76   S1293 - S1294   2022年10月   ISSN:0270-9139 eISSN:1527-3350

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  • Pathobiont-responsive Th17 cells in gut-mouth axis provoke inflammatory oral disease and are modulated by intestinal microbiome 国際誌

    Nagao, JI; Kishikawa, S; Tanaka, H; Toyonaga, K; Narita, Y; Negoro-Yasumatsu, K; Tasaki, S; Arita-Morioka, KI; Nakayama, J; Tanaka, Y

    CELL REPORTS   40 ( 10 )   111314 - 111314   2022年9月   ISSN:2211-1247

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Cell Reports  

    Host immune response via Th17 cells against oral pathobionts is a key mediator in periodontitis development. However, where and how the Th17-type immune response is induced during the development of periodontitis is not well understood. Here, we demonstrate that gut translocation of the oral pathobiont Porphyromonas gingivalis (Pg) exacerbates oral pathobiont-induced periodontitis with enhanced Th17 cell differentiation. The oral pathobiont-responsive Th17 cells are differentiated in Peyer's patches and translocated systemically in the peripheral immune tissues. They are also capable of migrating to and accumulating in the mouth upon oral infection. Development of periodontitis via the oral pathobiont-responsive Th17 cells is regulated by the intestinal microbiome, and altering the intestinal microbiome composition with antibiotics affects the development of periodontitis. Our study highlights that pathobiont-responsive Th17 cells in the gut-mouth axis and the intestinal microbiome work together to provoke inflammatory oral diseases, including periodontitis.

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  • The association between early formula and reduced risk of cow's milk allergy during the first three year of life: a Japanese cohort study 国際誌

    Ikari, K; Tezuka, J; Sanefuji, M; Nakayama, J; Nishima, D; Sonoda, Y; Ogawa, M; Shimono, M; Suga, R; Honjo, S; Kusuhara, K; Ohga, S

    ALLERGY ASTHMA AND CLINICAL IMMUNOLOGY   18 ( 1 )   71 - 71   2022年8月   ISSN:1710-1492

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Allergy, Asthma and Clinical Immunology  

    BACKGROUND: Our recent observational study showed that regular consumption of cow's milk (CM) formula during early infancy (3-6 months old) was associated with a reduced risk of CM allergy (CMA) at 12 months old. However, the long-term association is unclear. The present study was aimed to examine how long this inverse association persists after 12 months old. METHODS: This study used the dataset of an ongoing nationwide prospective cohort, the Japan Environment and Children's Study, in which participants were registered between January 2011 and March 2014. We analyzed 65,568 children followed-up until 36 months old. The exposure factors were the consumption statuses of formula milk from 0-3, 3-6, and 6-12 months old. The primary outcome was the prevalence of CMA at 6, 12, 18, 24 and 36 months old. CMA was defined as an allergic reaction and sensitization to CM protein in an individual with no or limited intake of this protein at the evaluation time, combined with physician-diagnosed food allergy. Multivariable regression models were used to estimate the association between the periods of formula consumption and the prevalence of CMA. RESULTS: The prevalence of CMA increased with a peak of 1.51% at 18 months old and then declined to 0.79% at 36 months old. Formula milk from 3-6 months old was associated with a reduced risk of CMA throughout the first 3 years of life, although the extent of the reduction was mitigated with age (adjusted relative risk: [95% confidence interval]: 0.19 [0.10-0.34] at 12 months old, 0.23 [0.16-0.33] at 18 months old, 0.41 [0.26-0.64] at 24 months old, and 0.47 [0.26-0.80] at 36 months old). The association between early formula and CMA were observed in both children with and without eczema, but more prominent and long-lasting in the former than the latter. CONCLUSIONS: Regular exposure to CM protein during infancy was associated with a reduced prevalence of CMA during early childhood. At present, however, this observational study does not necessarily encourage formula feeding, and randomized controlled trials are warranted to confirm the findings and their significance.

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  • Glucosylceramide Changes Bacterial Metabolism and Increases Gram-Positive Bacteria through Tolerance to Secondary Bile Acids In Vitro 査読 国際誌

    Dai, HH; Otsuka, A; Tanabe, K; Yanagita, T; Nakayama, J; Kitagaki, H

    INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES   23 ( 10 )   2022年5月   ISSN:16616596 eISSN:1422-0067

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:International Journal of Molecular Sciences  

    Glucosylceramide is present in many foods, such as crops and fermented foods. Most glucosylceramides are not degraded or absorbed in the small intestine and pass through the large intestine. Glucosylceramide exerts versatile effects on colon tumorigenesis, skin moisture, cholesterol metabolism and improvement of intestinal microbes in vivo. However, the mechanism of action has not yet been fully elucidated. To gain insight into the effect of glucosylceramide on intestinal microbes, glucosylceramide was anaerobically incubated with the dominant intestinal microbe, Blautia coccoides, and model intestinal microbes. The metabolites of the cultured broth supplemented with glucosylceramide were significantly different from those of broth not treated with glucosylceramide. The number of Gram-positive bacteria was significantly increased upon the addition of glucosylceramide compared to that in the control. Glucosylceramide endows intestinal microbes with tolerance to secondary bile acid. These results first demonstrated that glucosylceramide plays a role in the modification of intestinal microbes.

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  • Characterization and Demonstration of Mock Communities as Control Reagents for Accurate Human Microbiome Community Measurements 査読 国際誌

    Tourlousse D.M., Narita K., Miura T., Ohashi A., Matsuda M., Ohyama Y., Shimamura M., Furukawa M., Kasahara K., Kameyama K., Saito S., Goto M., Shimizu R., Mishima R., Nakayama J., Hosomi K., Kunisawa J., Terauchi J., Sekiguchi Y., Kawasaki H.

    Microbiology Spectrum   10 ( 2 )   e0191521   2022年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Microbiology Spectrum  

    Standardization and quality assurance of microbiome community analysis by high-throughput DNA sequencing require widely accessible and well-characterized reference materials. Here, we report on newly developed DNA and whole-cell mock communities to serve as control reagents for human gut microbiota measurements by shotgun metagenomics and 16S rRNA gene amplicon sequencing. The mock communities were formulated as near-even blends of up to 20 bacterial species prevalent in the human gut, span a wide range of genomic guanine-cytosine (GC) contents, and include multiple strains with Gram-positive type cell walls. Through a collaborative study, we carefully characterized the mock communities by shotgun metagenomics, using previously developed standardized protocols for DNA extraction and sequencing library construction. Further, we validated fitness of the mock communities for revealing technically meaningful differences among protocols for DNA extraction and metagenome/16S rRNA gene amplicon library construction. Finally, we used the mock communities to reveal varying performance of metagenome-based taxonomic profilers and the impact of trimming and filtering of sequencing reads on observed species profiles. The latter showed that aggressive preprocessing of reads may result in substantial GC-dependent bias and should thus be carefully evaluated to minimize unintended effects on species abundances. Taken together, the mock communities are expected to support a myriad of applications that rely on well-characterized control reagents, ranging from evaluation and optimization of methods to assessment of reproducibility in interlaboratory studies and routine quality control. IMPORTANCE Application of high-throughput DNA sequencing has greatly accelerated human microbiome research and its translation into new therapeutic and diagnostic capabilities. Microbiome community analyses results can, however, vary considerably across studies or laboratories, and establishment of measurement standards to improve accuracy and reproducibility has become a priority. The here-developed mock communities, which are available from the NITE Biological Resource Center (NBRC) at the National Institute of Technology and Evaluation (NITE, Japan), provide well-characterized control reagents that allow users to judge the accuracy of their measurement results. Widespread and consistent adoption of the mock communities will improve reproducibility and comparability of microbiome community analyses, thereby supporting and accelerating human microbiome research and development.

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  • Characterization and Demonstration of Mock Communities as Control Reagents for Accurate Human Microbiome Community Measurements

    Tourlousse, DM; Narita, K; Miura, T; Ohashi, A; Matsuda, M; Ohyama, Y; Shimamura, M; Furukawa, M; Kasahara, K; Kameyama, K; Saito, S; Goto, M; Shimizu, R; Mishima, R; Nakayama, J; Hosomi, K; Kunisawa, J; Terauchi, J; Sekiguchi, Y; Kawasaki, H

    MICROBIOLOGY SPECTRUM   10 ( 2 )   2022年4月   ISSN:2165-0497

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  • Secondary Metabolites of Actinomycetales as Potent Quorum Sensing Inhibitors Targeting Gram-Positive Pathogens: In Vitro and In Silico Study 査読 国際誌

    Desouky, SE; Abu-Elghait, M; Fayed, EA; Selim, S; Yousuf, B; Igarashi, Y; Abdel-Wahab, BA; Alsuhaibani, AM; Sonomoto, K; Nakayama, J

    METABOLITES   12 ( 3 )   2022年3月   ISSN:2218-1989 eISSN:2218-1989

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Metabolites  

    Anti-virulence agents are non-bacteriostatic and non-bactericidal emerging therapeutic options which hamper the production of virulence factors in pathogenic flora. In Staphylococcus aureus and Enterococcus faecalis, regulation of virulence genes' expression occurs through the cyclic peptide-mediated accessory gene regulator (agr) and its ortholog fsr quorum sensing systems, respectively. In the present study, we screened a set of 54 actinomycetales secondary metabolites as novel anti-virulence compounds targeting quorum sensing system of the Gram-positive bacteria. The results indicated that four compounds, Phenalinolactones A-D, BU-4664LMe, 4,5-dehydrogeldamycin, and Questinomycin A, potentially inhibit the agr quorum sensing system and hemolytic activity of S. aureus. On the other hand, Decatromicin A and B, Okilactomycin, Rishirilide A, Abyssomicin I, and Rebeccamycin selectively blocked the fsr quorum sensing system and the gelatinase production in E. faecalis at sub-lethal concentrations. Interestingly, Synerazol uniquely showed the capability to inhibit both fsr and agr quorum sensing systems. Further, in silico molecular docking studies were performed which provided closer insights into the mode of action of these compounds and proposed that the inhibitory activity of these compounds could be attributed to their potential ability to bind to the ATP-active site of S. aureus AgrA. Taken together, our study highlights the potential of actinomycetales secondary metabolites with diverse structures as anti-virulence quorum sensing inhibitors.

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  • “胆汁酸”を介した腸内細菌と宿主のクロストーク

    田中 優, 中山 二郎

    化学と生物   60 ( 2 )   79 - 88   2022年2月   ISSN:0453073X eISSN:18836852

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    記述言語:日本語   出版者・発行元:公益社団法人 日本農芸化学会  

    DOI: 10.1271/kagakutoseibutsu.60.79

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  • Gut microbiome profiles in Thai healthy pregnant women and its association with types of foods

    Phoonlapdacha, P; Tangshewinsirikul, C; Phosuwattanakul, J; Kittisakmontri, K; Nitisinprasert, S; Nakayama, J; Prombutara, P; Suthutvoravut, U; Chongviriyaphan, N

    BMC PREGNANCY AND CHILDBIRTH   22 ( 1 )   79   2022年1月   eISSN:1471-2393

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    記述言語:英語   出版者・発行元:BMC Pregnancy and Childbirth  

    Background: Gut microbiome colonization during early life is significant for immunological and physiological development. Maternal microbiome is associated with proper development of infants. The aim of this study was to determine the gut microbiome profiles among Thai healthy pregnant women and its associated factors. Methods: A multicenter, open trial prospective study was performed at three hospitals in Northern, Central, and Northeastern regions of Thailand. Thai healthy pregnant women attending antenatal clinics were recruited. Fecal samples of subjects at the third trimester of pregnancy were collected with sterilized techniques. The gut microbiome profiles and bacterial diversity were assessed using 16Ss RNA gene sequencing. Demographic data, dietary intake, and anthropometric data were recorded and analyzed. Results: There were 86 healthy pregnant women. The dominant of gut microbiome profiles were Bacteroidetes and Firmicutes. Pregnant women in the Central region had significantly higher of Ruminococcaceae and Lachnospiraceae than those in other regions (p < 0.001). Pregnant women in the Northern region significantly consumed more glutinous rice than those in other regions (p < 0.001). Glutinous rice intake was positively correlated with Bacteroidetes (rho = 0.405, p = 0.01) and negatively correlated with Firmicutes (rho = − 0.440, p = 0.001). Alpha diversity was not correlated with pre-pregnancy body mass index (BMI) or gestational weight gain. Conclusions: The gut microbiome profiles mainly found in Thai healthy pregnant women were Bacteroidetes and Firmicutes. The gut microbiome profiles in pregnant women found in this study possibly depended on dietary patterns. Glutinous rice with high amylopectin is probably related to abundance of Bacteroidetes.

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  • Bile acid dysmetabolism in the gut-microbiota-liver axis under hepatitis C virus infection 査読 国際誌

    Inoue, T; Funatsu, Y; Ohnishi, M; Isogawa, M; Kawashima, K; Tanaka, M; Moriya, K; Kawaratani, H; Momoda, R; Iio, E; Nakagawa, H; Suzuki, Y; Matsuura, K; Fujiwara, K; Nakajima, A; Yoshiji, H; Nakayama, J; Tanaka, Y

    LIVER INTERNATIONAL   42 ( 1 )   124 - 134   2022年1月   ISSN:1478-3223 eISSN:1478-3231

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Liver International  

    BACKGROUND & AIMS: We recently analysed and reported the features of the micro biome under hepatitis C virus (HCV) infection, but the effect of HCV infection on bile acid (BA) metabolism in the gut-liver axis remains poorly understood. The aim of this study was to clarify the characteristics of the gut-liver axis in HCV-infected patients. METHODS: The faecal BAs composition and gut microbiota from 100 chronic hepatitis C (CHC) patients were compared with those from 23 healthy individuals. For transcriptional analysis of the liver, 22 mild CHC (fibrosis stages [F] 0-2) and 42 advanced CHC (F3-4) cases were compared with 12 healthy individuals. The findings were confirmed using chimeric mice with human hepatocytes infected with HCV HCR6. RESULTS: Chronic hepatitis C patients, even at earlier disease stages, showed BA profiles distinct from healthy individuals, in which faecal deoxycholic acid (DCA) was significantly reduced and lithocholic acid or ursodeoxycholic acid became dominant. The decrease in faecal DCA was correlated with reduction in commensal Clostridiales and increase in oral Lactobacillales. Impaired biosynthesis of cholic acid (CA) was observed as a reduction in the transcription level of cytochrome P450 8B1 (CYP8B1), a key enzyme in CA biosynthesis. The reductions in faecal DCA and liver CYP8B1 were also observed in HCV-infected chimeric mice. CONCLUSIONS: Chronic hepatitis C alters the intestinal BA profile, in association with the imbalance of BA biosynthesis, which differs from the pattern in NAFLD. These imbalances appear to drive disease progression through the gut-microbiome-liver axis.

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  • Crisis of the Asian gut: associations among diet, microbiota, and metabolic diseases

    Therdtatha, P; Shinoda, A; Nakayama, J

    BIOSCIENCE OF MICROBIOTA FOOD AND HEALTH   41 ( 3 )   83 - 93   2022年   ISSN:2186-6953 eISSN:21863342

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:BMFH出版会  

    The increase of lifestyle-related diseases in Asia has recently become remarkably serious. This has been associated with a change in dietary habits that may alter the complex gut microbiota and its metabolic function in Asian people. Notably, the penetration of modern Western diets into Asia, which has been accompanied by an increase in fat content and decrease in plant-derived dietary fiber, is restructuring the Asian gut microbiome. In this review, we introduce the current status of obesity and diabetes in Asia and discuss the links of changes in dietary style with gut microbiota alterations which may predispose Asian people to metabolic diseases.

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  • Characterization of the Biosynthetic Gene Cluster of Enterocin F4-9, a Glycosylated Bacteriocin. 査読 国際誌

    Mohamed Abdelfattah Maky, Naoki Ishibashi, Jiro Nakayama, Takeshi Zendo

    Microorganisms   9 ( 11 )   2021年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Enterocin F4-9 belongs to the glycocin family having post-translational modifications by two molecules of N-acetylglucosamine β-O-linked to Ser37 and Thr46. In this study, the biosynthetic gene cluster of enterocin F4-9 was cloned and expressed in Enterococcus faecalis JH2-2. Production of glycocin by the JH2-2 expression strain was confirmed by expression of the five genes. The molecular weight was greater than glycocin secreted by the wild strain, E. faecalis F4-9, because eight amino acids from the N-terminal leader sequence remained attached. This N-terminal extension was eliminated after treatment with the culture supernatant of strain F4-9, implying an extracellular protease from E. faecalis F4-9 cleaves the N-terminal sequence. Thus, leader sequences cleavage requires two steps: the first via the EnfT protease domain and the second via extracellular proteases. Interestingly, the long peptide, with N-terminal extension, demonstrated advanced antimicrobial activity against Gram-positive and Gram-negative bacteria. Furthermore, enfC was responsible for glycosylation, a necessary step prior to secretion and cleavage of the leader peptide. In addition, enfI was found to grant self-immunity to producer cells against enterocin F4-9. This report demonstrates specifications of the minimal gene set responsible for production of enterocin F4-9, as well as a new biosynthetic mechanism of glycocins.

    DOI: 10.3390/microorganisms9112276

  • Mechanistic Insight into Yeast Bloom in a Lactic Acid Bacteria Relaying-Community in the Start of Sourdough Microbiota Evolution. 査読 国際誌

    Mugihito Oshiro, Masaru Tanaka, Rie Momoda, Takeshi Zendo, Jiro Nakayama

    Microbiology spectrum   9 ( 2 )   e0066221   2021年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    The spontaneous microbiota of wheat sourdough, often comprising one yeast species and several lactic acid bacteria (LAB) species, evolves over repeated fermentation cycles, which bakers call backslopping. The final product quality largely depends on the microbiota functions, but these fluctuate sometimes during the initial months of fermentation cycles due to microbiota evolution in which three phases of LAB relay occur. In this study, the understanding of yeast-LAB interactions in the start of the evolution of the microbiota was deepened by exploring the timing and trigger interactions when sourdough yeast entered a preestablished LAB-relaying community. Monitoring of 32 cycles of evolution of 6 batches of spontaneous microbiota in wheat sourdoughs revealed that sourdough yeasts affected the LAB community when the 2nd- or 3rd-relaying types of LAB genera emerged. In in vitro pairwise cocultures, all 12 LAB strains containing the 3 LAB-relaying types arrested the growth of a Saccharomyces cerevisiae strain, a frequently found species in sourdoughs, to various extents by sugar-related interactions. These findings suggest competition due to different affinities of each LAB and a S. cerevisiae strain for each sugar. In particular, maltose was the driver of S. cerevisiae growth in all pairwise cocultures. The functional prediction of sugar metabolism in sourdough LAB communities showed a positive correlation between maltose degradation and the yeast population. Our results suggest that maltose-related interactions are key factors that enable yeasts to enter and then settle in the LAB-relaying community during the initial part of evolution of spontaneous sourdough microbiota. IMPORTANCE Unpredictable evolution of spontaneous sourdough microbiota sometimes prevents bakers from making special-quality products because the unstable microbiota causes the product quality to fluctuate. Elucidation of the evolutionary mechanisms of the sourdough community, comprising yeast and lactic acid bacteria (LAB), is fundamental to control fermentation performance. This study investigated the mechanisms by which sourdough yeasts entered and settled in a bacterial community in which a three-phase relay of LAB occurred. Our results showed that all three layers of LAB restricted the cohabiting yeast population by competing for the sugar sources, particularly maltose. During the initial evolution of spontaneous sourdough microbiota, yeasts tended to grow synchronously with the progression of the lactic acid bacterial relay, which was predictably associated with changes in the maltose degradation functions in the bacterial community. Further study of ≥3 species' interactions while considering yeast diversity can uncover additional interaction mechanisms driving the initial evolution of sourdough microbiota.

    DOI: 10.1128/Spectrum.00662-21

  • Cultivation of Spore-Forming Gut Microbes Using a Combination of Bile Acids and Amino Acids. 査読 国際誌

    Sakura Onizuka, Masaru Tanaka, Riko Mishima, Jiro Nakayama

    Microorganisms   9 ( 8 )   2021年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Spores of certain species belonging to Firmicutes are efficiently germinated by nutrient germinators, such as amino acids, in addition to bile acid. We attempted to culture difficult-to-culture or yet-to-be cultured spore-forming intestinal bacteria, using a combination of bile acids and amino acids. The combination increased the number of colonies that formed on agar medium plated with ethanol-treated feces. The operational taxonomic units of these colonized bacteria were classified into two types. One type was colonized only by the bile acid (BA) mixture and the other type was colonized using amino acids, in addition to the BA mixture. The latter contained 13 species, in addition to 14 species of the former type, which mostly corresponds to anaerobic difficult-to-culture Clostridiales species, including several new species candidates. The use of a combination of BAs and amino acids effectively increased the culturability of spore-forming intestinal bacteria.

    DOI: 10.3390/microorganisms9081651

  • Mucosa-associated gut microbiota reflects clinical course of ulcerative colitis. 査読 国際誌

    Yuichiro Nishihara, Haruei Ogino, Masaru Tanaka, Eikichi Ihara, Keita Fukaura, Kei Nishioka, Takatoshi Chinen, Yoshimasa Tanaka, Jiro Nakayama, Dongchon Kang, Yoshihiro Ogawa

    Scientific reports   11 ( 1 )   13743 - 13743   2021年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    This longitudinal study was designed to elucidate whether gut microbiota is associated with relapse and treatment response in ulcerative colitis (UC) patients. Fifty-one patients with UC were enrolled between 2012 and 2017, and followed up through 2020. Colon mucosal biopsy were obtained at enrollment, and 16S ribosomal RNA sequencing was performed using extracted RNA. Of the 51 patients, 24 were in remission and 27 had active UC at enrollment. Of the 24 patients in remission, 17 maintained remission and 7 developed relapse during follow-up. The 7 patients with relapse showed lower diversity, with a lower proportion of Clostridiales (p = 0.0043), and a higher proportion of Bacteroides (p = 0.047) at enrollment than those without relapse. The 27 patients with active UC were classified into response (n = 6), refractory (n = 13), and non-response (n = 8) groups according to their treatment response in 6 months. The refractory and non-response groups showed lower diversity with a lower proportion of Prevotella (p = 0.048 and 0.043) at enrollment than the response group. This study is the first demonstration that reduced diversity and particular microbes are associated with the later clinical course of relapse events and treatment response in UC.

    DOI: 10.1038/s41598-021-92870-0

  • Diversity and dynamics of sourdough lactic acid bacteriota created by a slow food fermentation system. 査読

    Mugihito Oshiro, Takeshi Zendo, Jiro Nakayama

    Journal of bioscience and bioengineering   131 ( 4 )   333 - 340   2021年4月

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    記述言語:英語  

    Sourdough is a naturally fermented dough that is used worldwide to produce a variety of baked foods. Various lactic acid bacteria (LAB), which can determine the quality of sourdough baked foods by producing metabolites, have been found in the sourdough ecosystem. However, spontaneous fermentation of sourdough leads to unpredictable growth of various micro-organisms, which result in unstable product quality. From an ecological perspective, many researchers have recently studied sourdough LAB diversity, particularly the elucidation of LAB community interactions and the dynamic mechanisms during the fermentation process, in response to requests for the control and design of a desired sourdough microbial community. This article reviews recent advances in the study of sourdough LAB diversity and its dynamics in association with unique characteristics of the fermentation system; it also discusses future perspectives for better understanding of the complex sourdough microbial ecosystem, which can be attained efficiently by both in vitro and in situ experimental approaches.

    DOI: 10.1016/j.jbiosc.2020.11.007

  • Molecular characterization of the possible regulation of multiple bacteriocin production through a three-component regulatory system in Enterococcus faecium NKR-5-3 査読 国際誌

    Naoki Ishibashi, Naho Matsumoto, Rodney Honrada Perez, Shun Iwatani, Haruki Sugino, Takeshi Zendo, Pongtep Wilaipun, Vichien Leelawatcharamas, Jiro Nakayama, Kenji Sonomoto

    Journal of Bioscience and Bioengineering   131 ( 2 )   131 - 138   2021年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Enterococcus faecium NKR-5-3 produces multiple-bacteriocins, enterocins NKR-5-3A, B, C, D, and Z (Ent53A, Ent53B, Ent53C, Ent53D, and Ent53Z). However, the biosynthetic mechanisms on how their productions are regulated are yet to be fully understood. In silico analysis revealed putative promoters and terminators in the enterocin NKR-5-3ACDZ gene cluster, and the putative direct repeats (5′-ATTTTAGGATA-3′) were conserved upstream of each promoter. Transcriptional analysis by quantitative real-time polymerase chain reaction (PCR) of the biosynthetic genes for the enterocins NKR-5-3 suggested that an inducing peptide (Ent53D) regulates the transcription of the structure genes and corresponding biosynthetic genes of enterocins NKR-5-3, except for Ent53B (a circular bacteriocin), thus consequently regulating their production. Moreover, transcriptional analysis of some knock-out mutants showed that the production of Ent53A, C, D and Z is controlled by a three-component regulatory system (TCS) consisting of Ent53D, EnkR (response regulator), and EnkK (histidine kinase). The production of the circular bacteriocin Ent53B appeared to be independent from this TCS. Nevertheless, disrupting the TCS by deletion of a single component (enkD, enkR and enkK) resulted in a slight increase of enkB transcription and consequently the production of Ent53B, presumably, as an indirect consequence of the increase of available energy to the strain NKR-5-3. Here, we demonstrate the regulatory control of the multiple bacteriocin production of strain NKR-5-3 likely through the TCS consisting of Ent53D, EnkR, and EnkK. The information of the sharing of the regulatory machinery between bacteriocins in strain NKR-5-3 can be useful in its future application such as designing strategies to effectively dispense its multiple bacteriocin arsenal.

    DOI: 10.1016/j.jbiosc.2020.09.019

  • The salivary microbiota of Thai adults with metabolic disorders and association with diet. 査読 国際誌

    Tawatchai Chumponsuk, Lucsame Gruneck, Eleni Gentekaki, Paiboon Jitprasertwong, Niwed Kullawong, Jiro Nakayama, Siam Popluechai

    Archives of oral biology   122   105036 - 105036   2021年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    OBJECTIVE: This study aimed to investigate abundance of specific bacterial taxa in the saliva of 105 Thai adults with different BMI (lean, overweight, and obese) and T2DM subjects using qPCR targeting the 16S rRNA gene of various bacteria taxa. DESIGN: We employed qPCR targeting 16S rRNA genes to explore the bacterial profiles and abundances in the saliva of Thai adult subjects with different BMI and T2DM. Multivariate statistical analyses (multiple factor analysis (MFA) and sparse Partial Least Squares Discriminant Analysis (sPLS-DA) were performed to assess the associations of salivary bacteria with diet, blood profile, gender, age, and use of antibiotics. RESULTS: We found that abundance profiles of the examined salivary bacteria were similar across the four groups. When diet, blood profile, and gender, age, and use of antibiotics were considered, significant differences were noted between subgroups. A positive correlation was also found between consumption of carbonate soft drinks and Bacteroidetes, Gamma-proteobacteria, Veillonella, Fusobacterium and Fusobacterium nucleatum. CONCLUSIONS: This is the first study demonstrating the relative abundance of salivary bacteria in adult Thai subjects with different levels of BMI and T2DM. Regardless of the similar pattern of bacterial profiles across groups, sPLS-DA analysis highlighted the influence of host variables (gender, age, and use of antibiotics) on the abundance of salivary microbiota. Our findings pave the way for further hypothesis testing to gain insight into the association between host factors and salivary microbiome.

    DOI: 10.1016/j.archoralbio.2020.105036

  • Gut Microbiota and Short-Chain Fatty Acid Profile between Normal and Moderate Malnutrition Children in Yogyakarta, Indonesia. 査読 国際誌

    Rafli Zulfa Kamil, Agnes Murdiati, Mohammad Juffrie, Jiro Nakayama, Endang Sutriswati Rahayu

    Microorganisms   9 ( 1 )   1 - 15   2021年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Malnutrition has been associated with the gut microbiota composition and the gastrointestinal environment. This study aimed to evaluate whether there is a difference in the gut microbiota profile between the normal and undernutrition (considered moderate malnutrition) children and evaluate the gastrointestinal environment observed from the short-chain fatty acid (SCFA) profile. Ten days' observations were done between normal (n:13) and undernutrition (n:15) children. The subject's diet was recorded using a food record. Analysis of the gut microbiota was performed using 16S rRNA gene sequencing targeting the V3-V4 variables region, while the SCFA profile was analyzed using gas chromatography. The result shows that the undernutrition group's energy intake was lower than in the normal group. Although there was no difference in diversity index and overall gut composition, overexpression of the genera Methanobrevibacter, Anaerococcus, Eubacterium, and Succinivibrio was observed in the undernutrition group. Meanwhile, in the normal group, Ruminococcus and Fusobacterium were found. In both groups, there was also the dominant of Prevotella enterotype. Gastrointestinal conditions in the normal group tended to be more acidic compared to the undernutrition group. It occurs due to the high concentration of propionate and butyric acids.

    DOI: 10.3390/microorganisms9010127

  • Processing and secretion of non-cognate bacteriocins by EnkT, an ABC transporter from a multiple-bacteriocin producer, Enterococcus faecium NKR-5-3. 査読

    Hirotoshi Sushida, Miyuki Sakei, Rodney H Perez, Naoki Ishibashi, Takeshi Zendo, Pongtep Wilaipun, Vichien Leelawatcharamas, Jiro Nakayama, Kenji Sonomoto

    Journal of bioscience and bioengineering   130 ( 6 )   596 - 603   2020年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    EnkT is an ATP-binding cassette (ABC) transporter produced by Enterococcus faecium NKR-5-3, which is responsible for the secretion of multiple bacteriocins; enterocins NKR-5-3A, C, D, and Z (Ent53A, C, D, and Z). EnkT has been shown to possess a tolerant recognition mechanism that enables it to secrete the mature Ent53C from a chimeric precursor peptide containing the leader peptide moieties that are derived from different heterologous bacteriocins. In this study, to further characterize EnkT, we aimed to investigate the capacity of EnkT to recognize, process, and secrete non-cognate bacteriocins, which belong to different subclasses of class II. For this, the non-cognate bacteriocin precursor peptides, including enterocin A, pediocin PA-1, lactococcin Q, lactococcin A, and lacticin Q were co-expressed with EnkT, and thereafter, the production of the mature forms of these non-cognate bacteriocins was assessed. Our results revealed that EnkT could potentially recognize, process, and secrete the non-cognate bacteriocins with an exception of the leaderless bacteriocin, lacticin Q. Moreover, the processing and secretion efficiencies of these heterologous non-cognate bacteriocins by EnkT were further enhanced when the leader peptide moiety was replaced with the Ent53C leader peptide (derived from a native NKR-5-3 bacteriocin). The findings of this study describe the wide substrate tolerance of this ABC transporter, EnkT, that can be exploited in the future in establishing effective bacteriocin production systems adaptive to complex fermentation conditions common in many food systems.

    DOI: 10.1016/j.jbiosc.2020.07.017

  • Alteration in faecal bile acids, gut microbial composition and diversity after laparoscopic sleeve gastrectomy 査読 国際誌

    T. Ikeda, M. Aida, Y. Yoshida, S. Matsumoto, M. Tanaka, J. Nakayama, Y. Nagao, R. Nakata, E. Oki, T. Akahoshi, S. Okano, M. Nomura, M. Hashizume, Y. Maehara

    British Journal of Surgery   107 ( 12 )   1673 - 1685   2020年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Background: Laparoscopic sleeve gastrectomy (LSG) is a well established treatment for severe obesity and type 2 diabetes. Although the gut microbiota is linked to the efficacy of LSG, the underlying mechanisms remain elusive. The effect of LSG for morbid obesity on the gut microbiota and bile acids was assessed here. Methods: Severely obese subjects who were candidates for LSG were included and followed until 6 months after surgery. The composition and abundance of the microbiota and bile acids in faeces were assessed by 16S ribosomal RNA sequencing, quantitative PCR and liquid chromatography–mass spectrometry. Results: In total, 28 patients with a mean(s.d.) BMI of 44·2(6·6) kg/m were enrolled. These patients had achieved excess weight loss of 53·2(19·0) per cent and showed improvement in metabolic diseases by 6 months after LSG, accompanied by an alteration in the faecal microbial community. The increase in α-diversity and abundance of specific taxa, such as Rikenellaceae and Christensenellaceae, was strongly associated with reduced faecal bile acid levels. These changes had a significant positive association with excess weight loss and metabolic alterations. However, the total number of faecal bacteria was lower in patients before (mean(s.d.) 10·26(0·36) log cells per g faeces) and after (10·39(0·29) log cells per g faeces) operation than in healthy subjects (10·83(0·27) log cells per g faeces). Conclusion: LSG is associated with a reduction in faecal bile acids and greater abundance of specific bacterial taxa and α-diversity that may contribute to the metabolic changes. 2 10 10 10

    DOI: 10.1002/bjs.11654

  • Indonesian children fecal microbiome from birth until weaning was different from microbiomes of their mothers. 査読 国際誌

    Wei Wei Thwe Khine, Endang Sutriswati Rahayu, Ting Yi See, Sherwin Kuah, Seppo Salminen, Jiro Nakayama, Yuan-Kun Lee

    Gut microbes   12 ( 1 )   1761240 - 1761240   2020年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Gastrointestinal (GI) microbiota play an important role in human health and wellbeing and the first wave of gut microbes arrives mostly through vertical transmission from mother to child. This study has undertaken to understand the microbiota profile of healthy Southeast Asian mother-infant pairs. Here, we examined the fecal, vaginal and breast milk microbiota of Indonesian mothers and the fecal microbiota of their children from less than 1 month to 48 months old. To determine the immune status of children and the effect of diet at different ages, we examined the level of cytokines, bile acids in the fecal water and weaning food frequency. The fecal microbiota of the children before weaning contained mainly Bacteroides and Bifidobacterium, which presented at low abundance in the samples of mothers. After weaning, the fecal microbiome of children was mainly of the Prevotella type, with decreasing levels of Bifidobacterium, thus becoming more like the fecal microbiome of the mother. The abundance of infant fecal commensals generally correlated inversely with potential pathogens before weaning. The fecal Bifidobacterium in children correlated inversely with the consumption of complex carbohydrates and fruits after weaning. The specific cytokines related to the proliferation and maturation of immunity were found to increase after weaning. A decreasing level of primary bile acids and an increase of secondary bile acids were observed after weaning. This study highlights the change in the GI microbiota of infants to adult-type microbiota after weaning and identifies diet as a major contributing factor.

    DOI: 10.1080/19490976.2020.1761240

  • Gut dysbiosis associated with clinical prognosis of patients with primary biliary cholangitis. 査読 国際誌

    Masanori Furukawa, Kei Moriya, Jiro Nakayama, Takako Inoue, Rie Momoda, Hideto Kawaratani, Tadashi Namisaki, Shinya Sato, Akitoshi Douhara, Kosuke Kaji, Mitsuteru Kitade, Naotaka Shimozato, Yasuhiko Sawada, Soichiro Saikawa, Hiroaki Takaya, Koh Kitagawa, Takemi Akahane, Akira Mitoro, Junichi Yamao, Yasuhito Tanaka, Hitoshi Yoshiji

    Hepatology research : the official journal of the Japan Society of Hepatology   50 ( 7 )   840 - 852   2020年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    AIM: Although some relationships between gut microbiota and liver diseases have been reported, it remains uncertain whether changes in gut microbiota owing to differences in race, food and living environment have similar effects. Response to ursodeoxycholic acid (UDCA) may predict the long-term prognosis of patients with primary biliary cholangitis (PBC); however, little is known about the significance of the gut microbiome in patients with PBC. We elucidated the relationships among clinical profiles, biochemical response to UDCA and gut microbiome composition in patients with PBC. METHODS: Fecal samples from 76 patients with PBC treated at our hospital were collected; patients whose UDCA intake period was <1 year were excluded. The microbiome structures of patients were determined using 16S ribosomal RNA gene sequencing and were statistically compared with those of healthy subjects. The structures of patients in the UDCA responder (n = 43) and non-responder (n = 30) groups were compared according to the Nara criteria (reduction rate of gamma-glutamyl transpeptidase, ≥69%, after 1 year). RESULTS: Compared with healthy subjects, bacterial diversity was lower in patients with PBC, with a decreased abundance of the order Clostridiales and increased abundance of Lactobacillales. The UDCA non-responder group had a significantly lower population of the genus Faecalibacterium, known as butyrate-producing beneficial bacteria (P < 0.05), although no significant differences in gender, body mass index, medicated drugs or other serological data were indicated between these two groups. CONCLUSIONS: Gut dysbiosis with loss of beneficial Clostridiales commensals was observed in patients with PBC. Decrease in Faecalibacterium abundance might predict the long-term prognosis of patients with PBC.

    DOI: 10.1111/hepr.13509

  • Impact of pH on succession of sourdough lactic acid bacteria communities and their fermentation properties 査読 国際誌

    Mugihito OSHIRO, Masaru TANAKA, Takeshi ZENDO, Jiro NAKAYAMA

    Bioscience of Microbiota, Food and Health   39 ( 3 )   152 - 159   2020年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.12938/bmfh.2019-038

  • Age-related changes in the gut microbiota and the core gut microbiome of healthy Thai humans. 査読 国際誌

    Orawan La-Ongkham, Massalin Nakphaichit, Jiro Nakayama, Suttipun Keawsompong, Sunee Nitisinprasert

    3 Biotech   10 ( 6 )   276 - 276   2020年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    The gut microbial diversity of Thai people was investigated between two large cohorts, adult and elderly subjects, from the middle region of Thailand; the cohorts were divided into different age groups of healthy adult (73) and elderly subjects (47). The diversities of the groups were characterized using a pyrosequencing technique with primers targeting the V6-V8 region of the 16S rRNA gene, and a significant decrease in the Firmicutes and Bacteroidetes ratio from 7.3 to 4.5 was observed with increased age. The microbiota of the adult and elderly groups had a significantly higher abundance of the phylum Actinobacteria, including the three species Bifidobacterium adolescentis, Bifidobacterium longum and Bifidobacterium pseudocatenulatum, and the phylum Bacteroidetes containing the four species Bacteroides uniformis, Bacteroides ovatus, Bacteroides caccae and Bacteroides thetaiotaomicron. Firmicutes showed no significant differences between the two groups. Eleven species belonging to Firmicutes, Bacteroidetes and Proteobacteria were shared by at least 90% of all subjects and defined as core gut microbiota of healthy Thai, among which a high abundance of Escherichia coli was particularly characterized in Thai elderly individuals. Multiple linear regression analysis of age, gender, BMI and diet consumption frequency showed the correlation of age with Bacteroides and Bifidobacterium. Rice consumption frequency showed a significant positive correlation with Bacteroides, while no correlation was found for other factors. Taken together, in the gut of Thai adults, Bifidobacterium decreased and Bacteroides increased with age, while rice consumption increased the abundance of Bacteroides. These link of age and food, especially rice carbohydrate, to gut microbiota and health could be ultimately proposed as the Thai feature.

    DOI: 10.1007/s13205-020-02265-7

  • Age-related changes in the gut microbiota and the core gut microbiome of healthy Thai humans. 査読 国際誌

    Orawan La-Ongkham, Massalin Nakphaichit, Jiro Nakayama, Suttipun Keawsompong, Sunee Nitisinprasert

    3 Biotech   10 ( 6 )   276 - 276   2020年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    The gut microbial diversity of Thai people was investigated between two large cohorts, adult and elderly subjects, from the middle region of Thailand; the cohorts were divided into different age groups of healthy adult (73) and elderly subjects (47). The diversities of the groups were characterized using a pyrosequencing technique with primers targeting the V6-V8 region of the 16S rRNA gene, and a significant decrease in the Firmicutes and Bacteroidetes ratio from 7.3 to 4.5 was observed with increased age. The microbiota of the adult and elderly groups had a significantly higher abundance of the phylum Actinobacteria, including the three species Bifidobacterium adolescentis, Bifidobacterium longum and Bifidobacterium pseudocatenulatum, and the phylum Bacteroidetes containing the four species Bacteroides uniformis, Bacteroides ovatus, Bacteroides caccae and Bacteroides thetaiotaomicron. Firmicutes showed no significant differences between the two groups. Eleven species belonging to Firmicutes, Bacteroidetes and Proteobacteria were shared by at least 90% of all subjects and defined as core gut microbiota of healthy Thai, among which a high abundance of Escherichia coli was particularly characterized in Thai elderly individuals. Multiple linear regression analysis of age, gender, BMI and diet consumption frequency showed the correlation of age with Bacteroides and Bifidobacterium. Rice consumption frequency showed a significant positive correlation with Bacteroides, while no correlation was found for other factors. Taken together, in the gut of Thai adults, Bifidobacterium decreased and Bacteroides increased with age, while rice consumption increased the abundance of Bacteroides. These link of age and food, especially rice carbohydrate, to gut microbiota and health could be ultimately proposed as the Thai feature.

    DOI: 10.1007/s13205-020-02265-7

  • Relation between cell-bound exopolysaccharide production via plasmid-encoded genes and rugose colony morphology in the probiotic Lactobacillus brevis KB290. 査読 国際誌

    Masanori Fukao, Takeshi Zendo, Takuro Inoue, Nobuo Fuke, Tomoo Moriuchi, Yasuhiro Yamane, Jiro Nakayama, Kenji Sonomoto, Tetsuya Fukaya

    Animal science journal = Nihon chikusan Gakkaiho   90 ( 12 )   1575 - 1580   2019年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    The probiotic Lactobacillus brevis KB290 is a natural producer of cell-bound exopolysaccharide (EPS), and the plasmid-encoded glycosyltransferase genes are responsible for this EPS production. KB290 forms unique rugose colonies inside an agar medium; this characteristic is useful for detecting and enumerating KB290 in the gut or feces. However, the genetic elements associated with this morphology remain unclear. Here, we aimed to investigate the relation between the plasmid eps genes and rugose colony morphology in KB290. The plasmid-cured mutants formed smooth colonies, and the rugose colony morphology was restored after complementation with the eps genes. The eps genes were successfully cloned and expressed in other L. brevis and L. plantarum strains. In these transformant strains, the presence of the EPS, consisting of glucose and N-acetylglucosamine, correlated with rugose colonies, indicating that EPS is responsible for rugose colony formation. To the best of our knowledge, this is the first report identifying the genetic factors influencing rugose colonies in Lactobacillus strains. This rugose colony formation may serve as a useful selective marker for KB290 in routine laboratory and research settings and can be used to detect the spontaneous loss of plasmids in this strain.

    DOI: 10.1111/asj.13297

  • The gelatinase biosynthesis-activating pheromone binds and stabilises the FsrB membrane protein in Enterococcus faecalis quorum sensing 査読 国際誌

    Sean Littlewood, Helena Tattersall, Charlotte S. Hughes, Rohanah Hussain, Pikyee Ma, Stephen E. Harding, Jiro Nakayama, Mary K. Phillips-Jones

    FEBS Letters   594 ( 3 )   553 - 563   2019年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Quorum-sensing mechanisms regulate gene expression in response to changing cell-population density detected through pheromones. In Enterococcus faecalis, Fsr quorum sensing produces and responds to the gelatinase biosynthesis-activating pheromone (GBAP). Here we establish that the enterococcal FsrB membrane protein has a direct role connected with GBAP by showing that GBAP binds to purified FsrB. Far-UV CD measurements demonstrated a predominantly α-helical protein exhibiting a small level of conformational flexibility. Fivefold (400 μm) GBAP stabilised FsrB (80 μm) secondary structure. FsrB thermal denaturation in the presence and absence of GBAP revealed melting temperatures of 70.1 and 60.8 °C, respectively, demonstrating GBAP interactions and increased thermal stability conferred by GBAP. Addition of GBAP also resulted in tertiary structural changes, confirming GBAP binding.

    DOI: 10.1002/1873-3468.13634

  • Plasmid-encoded glycosyltransferase operon is responsible for exopolysaccharide production, cell aggregation, and bile resistance in a probiotic strain, Lactobacillus brevis KB290. 査読

    Masanori Fukao, Takeshi Zendo, Takuro Inoue, Jiro Nakayama, Shigenori Suzuki, Tetsuya Fukaya, Nobuhiro Yajima, Kenji Sonomoto

    Journal of bioscience and bioengineering   128 ( 4 )   391 - 397   2019年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    We demonstrate here that exopolysaccharide (EPS) production, cell aggregation, and bile resistance in Lactobacillus brevis KB290 are conferred by three eps genes (gtf27, gtf28, and orf29) located on the 42.4-kb plasmid pKB290-1. The predicted products of gtf27 and gtf28 belong to the membrane-bound glycosyltransferase family whereas the orf29 gene product showed homology with the ABC transporter. On in silico analysis, these genes were found to be widely distributed among lactobacilli from publicly available genomes and metagenomes, and their function is not yet elucidated. RT-PCR analysis showed that the eps genes were organised in an operon and their expression was markedly lower in arabinose- and xylose-containing media than in a glucose-containing medium. The three eps genes were cloned and expressed in homologous and heterologous strains. Considerably less EPS was produced by the plasmid-cured KB1802 strain than by the parental KB290 strain, whereas a similar amount was produced by the KB1802 strain expressing the three eps genes. The KB1802 strain expressing gtf27 and gtf28 but not orf29 did not produce EPS. Cell aggregation and bile resistance were also decreased in KB1802 strains but were complemented by eps genes. Moreover, the three eps genes conferred these phenotypes to a Lactobacillus plantarum strain. In conclusion, the three eps genes in pKB290-1 were sufficient for EPS biosynthesis with glucose and N-acetylglucosamine, and were responsible for cell aggregation and bile resistance. We consider these phenotypes to be at least partly responsible for KB290-specific properties.

    DOI: 10.1016/j.jbiosc.2019.04.008

  • Koji glycosylceramide commonly contained in Japanese traditional fermented foods alters cholesterol metabolism in obese mice. 査読 国際誌

    Hiroshi Hamajima, Masaru Tanaka, Miyuki Miyagawa, Mayuko Sakamoto, Tsuyoshi Nakamura, Teruyoshi Yanagita, Megumi Nishimukai, Susumu Mitsutake, Jiro Nakayama, Koji Nagao, Hiroshi Kitagaki

    Bioscience, biotechnology, and biochemistry   83 ( 8 )   1514 - 1522   2019年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Koji, which is manufactured by proliferating non-pathogenic fungus Aspergillus oryzae on steamed rice, is the base for Japanese traditional fermented foods. We have revealed that koji and related Japanese fermented foods and drinks such as amazake, shio-koji, unfiltered sake and miso contain abundant glycosylceramide. Here, we report that feeding of koji glycosylceramide to obese mice alters the cholesterol metabolism . Liver cholesterol was significantly decreased in obese mice fed with koji glycosylceramide. We hypothesized that their liver cholesterol was decreased because it was converted to bile acids. Consistent with the hypothesis, many bile acids were increased in the cecum and feces of obese mice fed with koji glycosylceramide. Expressions of CYP7A1 and ABCG8 involved in the metabolism of cholesterol were significantly increased in the liver of mice fed with koji glycosylceramide. Therefore, it was considered that koji glycosylceramide affects the cholesterol metabolism in obese mice.

    DOI: 10.1080/09168451.2018.1562877

  • Protective effect of lactobacillus reuteri KUB-AC5 against salmonella enteritidis challenge in chickens 査読 国際誌

    M. Nakphaichit, S. Sobanbua, S. Siemuang, W. Vongsangnak, Jiro Nakayama, S. Nitisinprasert

    Beneficial Microbes   10 ( 1 )   43 - 54   2019年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Poultry is an important high-quality food and protein source for humans. However, chicken is considered a primary source of foodborne diseases, especially Salmonella Enteritidis infection. Reducing Salmonella contamination in live poultry will thus lower the risk to consumers. Our previous studies reported that Lactobacillus reuteri KUB-AC5 can produce a substance with antimicrobial activity against pathogenic bacteria, especially Salmonella. In vivo testing revealed that this strain greatly influenced the ileal microbiota by improving chicken gastrointestinal health and inhibiting certain pathogenic bacteria. However, its activity against Salmonella in chicken is unknown. This study investigated the effects of the probiotic L. reuteri KUB-AC5 at various concentrations against Salmonella and the microbiota status in the gastrointestinal tract of broiler chickens. Four treatments groups were used: negative-control group (no Salmonella challenge), positive-control group (Salmonella challenge), and 5 or 7 log cfu probiotic supplementation to Salmonella-challenged chickens. The resultant microbial diversities at the growing and finisher stages were not significantly different among the groups (P>0.05). However, a high dosage of KUB-AC5 maintained similar microbial diversity in Salmonella-challenged chickens as observed in the non-challenged group in the early stage. The exposure Salmonella can affect the microbial diversity that consequently contributes to the disease progression in chicken. Low and high dosages of KUB-AC5 eliminated S. Enteritidis from the ileum and caecum at 14, 21 and 35 days of age. A high-dose of KUB-AC5 also enhanced Lactobacillaceae levels in the growing stage in both the ileum and caecum and suppressed Enterobacteriaceae levels in the finisher stage on day 35, whereas these effects were not observed in the low dose of KUB-AC5 or control groups. These results support the potential value of high-dose L. reuteri KUB-AC5 supplementation for three days after hatching in preventing Salmonella infection in chickens.

    DOI: 10.3920/BM2018.0034

  • Evaluation of leader peptides that affect the secretory ability of a multiple bacteriocin transporter, EnkT. 査読

    Hirotoshi Sushida, Naoki Ishibashi, Takeshi Zendo, Pongtep Wilaipun, Vichien Leelawatcharamas, Jiro Nakayama, Kenji Sonomoto

    Journal of bioscience and bioengineering   126 ( 1 )   23 - 29   2018年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    EnkT is a novel ATP-binding cassette (ABC) transporter responsible for secretion of four bacteriocins, enterocins NKR-5-3A, C, D, and Z (Ent53A, C, D, and Z), produced by Enterococcus faecium NKR-5-3. It is generally recognized that the secretion of a bacteriocin requires a dedicated ABC transporter, although molecular mechanisms of this secretion are yet to be revealed. In order to characterize the unique ability of EnkT to secrete multiple bacteriocins, the role of N-terminal leader peptides of bacteriocin precursors was evaluated using Ent53C precursor as a model. The 18-amino acid leader peptide of Ent53C (Lc) was modified by site-directed mutagenesis to generate various point mutations, truncations, or extensions, and substitutions with other leader peptides. The impact of these Lc mutations on Ent53C secretion was evaluated using a quantitative antimicrobial activity assay. We observed that Ent53C production increased with Ala substitution of the highly conserved C-terminal double glycine residues that are recognized as the cleavage site. In contrast, Ent53C antimicrobial activity decreased, with decrease in the length of the putative α-helix-forming region of Lc. Furthermore, EnkT recognized and transported Ent53C of the transformants possessing heterologous leader peptides of enterocin A, pediocin PA-1, brochocins A and B, and lactococcins Qα and Qβ. These results indicated that EnkT shows significant tolerance towards the sequence and length of leader peptides, to secrete multiple bacteriocins. This further demonstrates the functional diversity of bacteriocin ABC transporters and the importance of leader peptides as their recognition motif.

    DOI: 10.1016/j.jbiosc.2018.01.015

  • Developing anti-virulence chemotherapies by exploiting the diversity of microbial quorum sensing systems

    Basit Yousuf, Keika Adachi, Jiro Nakayama

    Biotechnological Applications of Quorum Sensing Inhibitors   151 - 208   2018年5月

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    記述言語:英語  

    Quorum sensing is a process of chemical communication that adjusts the genetic expression of certain important biological functions in a cell-density- dependent manner. Quorum sensing (QS) regulates important bacterial behaviors, such as production of virulence factors, formation of biofilm, and antibiotic resistance, via signaling molecules called autoinducers (AIs). Gram-negative bacteria typically use N-acyl homoserine lactones (AHLs) and S-adenosyl methionine (SAM) molecules as signaling molecules for communication with neighboring cells, while Gram-positive bacteria use oligopeptides for the same purpose. Autoinducer-2 (AI-2) is used as a signaling molecule by both Gram-negative and Gram-positive bacteria. These QS molecules are often involved in the expression of pathogenicity in different bacterial species. Because of the rapid emergence and dissemination of drug-resistant pathogens worldwide, antivirulence chemotherapies may be potential alternatives to the use of antibiotics, which kill bacteria but allow the emergence of resistance. Recently, successful strategies, employed for inhibition/ manipulation of QS signaling, has brought real excitement for identifying novel advances to combat these life-threatening pathogens. This review highlights the QS systems used by Gram-negative and Gram-positive bacterial species and discusses promising QS inhibitor (QSI) molecules that may aid in designing novel antimicrobial therapeutics. Utilization of different QS components in the design and development of novel biotechnological products, such as biosensors, engineered microbial consortia, and anticancer molecules, is also addressed.

    DOI: 10.1007/978-981-10-9026-4_9

  • Developing anti-virulence chemotherapies by exploiting the diversity of microbial quorum sensing systems

    Basit Yousuf, Keika Adachi, Jiro Nakayama

    Biotechnological Applications of Quorum Sensing Inhibitors   151 - 208   2018年5月

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    記述言語:英語  

    Quorum sensing is a process of chemical communication that adjusts the genetic expression of certain important biological functions in a cell-density- dependent manner. Quorum sensing (QS) regulates important bacterial behaviors, such as production of virulence factors, formation of biofilm, and antibiotic resistance, via signaling molecules called autoinducers (AIs). Gram-negative bacteria typically use N-acyl homoserine lactones (AHLs) and S-adenosyl methionine (SAM) molecules as signaling molecules for communication with neighboring cells, while Gram-positive bacteria use oligopeptides for the same purpose. Autoinducer-2 (AI-2) is used as a signaling molecule by both Gram-negative and Gram-positive bacteria. These QS molecules are often involved in the expression of pathogenicity in different bacterial species. Because of the rapid emergence and dissemination of drug-resistant pathogens worldwide, antivirulence chemotherapies may be potential alternatives to the use of antibiotics, which kill bacteria but allow the emergence of resistance. Recently, successful strategies, employed for inhibition/ manipulation of QS signaling, has brought real excitement for identifying novel advances to combat these life-threatening pathogens. This review highlights the QS systems used by Gram-negative and Gram-positive bacterial species and discusses promising QS inhibitor (QSI) molecules that may aid in designing novel antimicrobial therapeutics. Utilization of different QS components in the design and development of novel biotechnological products, such as biosensors, engineered microbial consortia, and anticancer molecules, is also addressed.

    DOI: 10.1007/978-981-10-9026-4_9

  • Evaluation of leader peptides that affect the secretory ability of a multiple bacteriocin transporter, EnkT 査読 国際誌

    Hirotoshi Sushida, Naoki Ishibashi, Takeshi Zendo, Pongtep Wilaipun, Vichien Leelawatcharamas, Jiro Nakayama, Kenji Sonomoto

    Journal of Bioscience and Bioengineering   2018年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    EnkT is a novel ATP-binding cassette (ABC) transporter responsible for secretion of four bacteriocins, enterocins NKR-5-3A, C, D, and Z (Ent53A, C, D, and Z), produced by Enterococcus faecium NKR-5-3. It is generally recognized that the secretion of a bacteriocin requires a dedicated ABC transporter, although molecular mechanisms of this secretion are yet to be revealed. In order to characterize the unique ability of EnkT to secrete multiple bacteriocins, the role of N-terminal leader peptides of bacteriocin precursors was evaluated using Ent53C precursor as a model. The 18-amino acid leader peptide of Ent53C (Lc) was modified by site-directed mutagenesis to generate various point mutations, truncations, or extensions, and substitutions with other leader peptides. The impact of these Lc mutations on Ent53C secretion was evaluated using a quantitative antimicrobial activity assay. We observed that Ent53C production increased with Ala substitution of the highly conserved C-terminal double glycine residues that are recognized as the cleavage site. In contrast, Ent53C antimicrobial activity decreased, with decrease in the length of the putative α-helix-forming region of Lc. Furthermore, EnkT recognized and transported Ent53C of the transformants possessing heterologous leader peptides of enterocin A, pediocin PA-1, brochocins A and B, and lactococcins Qα and Qβ. These results indicated that EnkT shows significant tolerance towards the sequence and length of leader peptides, to secrete multiple bacteriocins. This further demonstrates the functional diversity of bacteriocin ABC transporters and the importance of leader peptides as their recognition motif.

    DOI: 10.1016/j.jbiosc.2018.01.015

  • 16S rRNA Metagenomics of Asian Gut Microbiota 招待 査読 国際誌

    Juma Kisuse, Jiro Nakayama

    Understanding Host-Microbiome Interactions - An Omics Approach Omics of Host-Microbiome Association   71 - 81   2017年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    To characterize the diversity of gut microbial community structures of Asian people, Asian Microbiome Project (AMP) has been established. AMP notably aims to understand the linkage of their gut microbiota with diets and its impact on their health. To that end, AMP began with phase I which focused on the gut microbiota of school-age children who must follow the regional dietary habit. Stool samples were collected from 303 school-age children living in urban or rural regions in five countries spanning temperate and tropical areas of Asia. Bacterial compositions of those samples were determined by using the hypervariable sequences of 16S rRNA V6-V8 region analyzed by 454 pyrosequencing platform. Their community profiles were characterized into two enterotype-like clusters, each driven by Prevotella (P-type) or Bifidobacterium/Bacteroides (BB-type), respectively. Moreover, random forest analysis marked the participant country of residence through fecal species analysis by demonstrating accumulating gut microbiota. The predicted metagenomics using PICRUSt program has suggested overrepresentation of certain enzymes which may reflect their intestinal environment, such as amylase for nondigestible starch in P-type subjects and choloylglycine hydrolase for bile acid metabolism in BB-type subjects. Following this pilot study using 454 sequencing platform, MiSeq pair-end sequencing platform has been introduced into AMP. The MiSeq platform covered more than 99% of gut microbial community profile. Enterotyping was reproduced regardless of the read regions and taxonomy levels. Further study using the MiSeq 16S rRNA metagenomics is promising to gain deep insight of gut microbial community of Asian people.

    DOI: 10.1007/978-981-10-5050-3_6

  • In vitro synergistic activities of cefazolin and nisin A against mastitis pathogens. 査読

    Kohei Kitazaki, Shoko Koga, Kohei Nagatoshi, Koichi Kuwano, Takeshi Zendo, Jiro Nakayama, Kenji Sonomoto, Hitoshi Ano, Hiromu Katamoto

    The Journal of veterinary medical science   79 ( 9 )   1472 - 1479   2017年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    First-generation cephalosporins such as cefazolin (CEZ) have been widely used for mastitis treatment in dairy cattle. However, the use of antibiotics results in the presence of antibiotic residues in milk, which is used for human consumption. Nisin A, a bacteriocin produced by Lactococcus lactis, has been used as a broad-spectrum food preservative for over 50 years. Therefore, a combination of CEZ and nisin A might provide an extended activity spectrum against mastitis pathogens and reduce the antibiotic dose for mastitis treatment. This study aimed to evaluate the combined effect of CEZ and nisin A against mastitis pathogens using the checkerboard and time-kill assays. In the checkerboard assay, the CEZ-nisin A combination exhibited a synergistic effect against Staphylococcus aureus (n=20/20) and Enterococcus faecalis (n=13/18), and meanwhile exhibited a mostly additive effect against Staphylococcus intermedius (n=12/20), Streptococcus agalactiae (n=10/10), Streptococcus dysgalactiae (n=18/18), and Escherichia coli (n=14/18). There were no indifferent or antagonistic effects between CEZ and nisin A. In the time-kill assay, the CEZ-nisin A combination at 0.5 × or 1 × minimum inhibitory concentration exhibited synergistic reduction of bacterial growth by over 3 log10 colony forming units per ml relative to that observed with either antimicrobial substance alone. These results suggest that the CEZ-nisin A combination can be used for developing an intramammary infusion for mastitis treatment, with lower antibiotic concentrations than normal.

    DOI: 10.1292/jvms.17-0180

  • Asian gut microbiome 査読

    Jiro Nakayama, Heping Zhang, Yuan Kun Lee

    Science Bulletin   62 ( 12 )   816 - 817   2017年6月

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    記述言語:英語  

    DOI: 10.1016/j.scib.2017.04.001

  • Mutations near the cleavage site of enterocin NKR-5-3B prepeptide reveal new insights into its biosynthesis. 査読 国際誌

    Rodney H Perez, Haruki Sugino, Naoki Ishibashi, Takeshi Zendo, Pongtep Wilaipun, Vichien Leelawatcharamas, Jiro Nakayama, Kenji Sonomoto

    Microbiology (Reading, England)   163 ( 4 )   431 - 441   2017年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Enterocin NKR-5-3B (Ent53B) is a 64-residue novel circular bacteriocin synthesized from an 87-residue prepeptide. Albeit through a still unknown mechanism, the EnkB1234 biosynthetic enzyme complex processes the prepeptide to yield its mature active, circular form. To gain insights into the key region/residue that plays a role in Ent53 maturation, several mutations near the cleavage site on the precursor peptide were generated. The interaction of the precursor peptide and EnkB1234 appeared to be hydrophobic in nature. At the Leu1 position, only mutations with helix structure-promoting hydrophobic residues (Ala, Ile, Val or Phe) were able to yield the mature Ent53B derivative. In this study, we also highlight the possible conformation-stabilizing role of the Ent53B leader peptide on the precursor peptide for its interaction with its biosynthetic enzyme complex. Any truncations of the leader peptide moiety interfered in the processing of the prepeptide. However, when propeptides of other circular bacteriocins (circularin A, leucocyclicin Q or lactocyclicin Q) were cloned at the C-terminus of the leader peptide, EnkB1234 could not process them to yield a mature bacteriocin. Taken together, these findings offer new perspectives in our understanding of the possible molecular mechanism of the biosynthesis of this circular bacteriocin. These new perspectives will help advance our current understanding to eventually elucidate circular bacteriocin biosynthesis. Understanding the biosynthetic mechanism of circular bacteriocins will materialize their application potential.

    DOI: 10.1099/mic.0.000435

  • In vitro synergistic activities of cefazolin and nisin a against mastitis pathogens 査読

    Kohei Kitazaki, Shoko Koga, Kohei Nagatoshi, Koichi Kuwano, Takeshi Zendo, Jiro Nakayama, Kenji Sonomoto, Hitoshi Ano, Hiromu Katamoto

    Journal of Veterinary Medical Science   79 ( 9 )   1472 - 1479   2017年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    First-generation cephalosporins such as cefazolin (CEZ) have been widely used for mastitis treatment in dairy cattle. However, the use of antibiotics results in the presence of antibiotic residues in milk, which is used for human consumption. Nisin A, a bacteriocin produced by Lactococcus lactis, has been used as a broad-spectrum food preservative for over 50 years. Therefore, a combination of CEZ and nisin A might provide an extended activity spectrum against mastitis pathogens and reduce the antibiotic dose for mastitis treatment. This study aimed to evaluate the combined effect of CEZ and nisin A against mastitis pathogens using the checkerboard and time-kill assays. In the checkerboard assay, the CEZ-nisin A combination exhibited a synergistic effect against Staphylococcus aureus (n=20/20) and Enterococcus faecalis (n=13/18), and meanwhile exhibited a mostly additive effect against Staphylococcus intermedius (n=12/20), Streptococcus agalactiae (n=10/10), Streptococcus dysgalactiae (n=18/18), and Escherichia coli (n=14/18). There were no indifferent or antagonistic effects between CEZ and nisin A. In the time-kill assay, the CEZ-nisin A combination at 0.5 × or 1 × minimum inhibitory concentration exhibited synergistic reduction of bacterial growth by over 3 log10 colony forming units per ml relative to that observed with either antimicrobial substance alone. These results suggest that the CEZ-nisin A combination can be used for developing an intramammary infusion for mastitis treatment, with lower antibiotic concentrations than normal.

    DOI: 10.1292/jvms.17-0180

  • The effects of administration of the Lactobacillus gasseri strain CP2305 on quality of life, clinical symptoms and changes in gene expression in patients with irritable bowel syndrome 査読

    K. Nobutani, D. Sawada, S. Fujiwara, Y. Kuwano, K. Nishida, J. Nakayama, H. Kutsumi, T. Azuma, K. Rokutan

    Journal of Applied Microbiology   122 ( 1 )   212 - 224   2017年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Aims: To clarify the effects of Lactobacillus gasseri CP2305 (CP2305) on quality of life and clinical symptoms and its functional mechanisms in patients with irritable bowel syndrome (IBS). Methods and Results: After the patients were administered CP2305 daily for 4 weeks, the IBS-severity index score was significantly improved compared with that of the placebo group, and this improvement was accompanied by a reduction in health-related worry and changes in intestinal microbiota. The gene expression profiling of the peripheral blood leucocytes showed that CP2305 treatment significantly up-regulated genes related to eukaryotic initiation factor 2 (EIF2) signalling. Eighty-two genes were down-regulated in IBS patients compared with healthy controls. The expression of 23 of these genes exhibited a CP2305-dependent increase associated with an improvement in IBS severity. The majority of the restored genes were related to EIF2 signalling. Conclusions: CP2305 administration is a potential candidate therapeutic option for patients with IBS. Significance and Impact of the Study: Although probiotics have been proposed to benefit IBS patients, objective clinical evidence and elucidation of the functional mechanism remain insufficient. Our study demonstrated that CP2305 administration beneficially influences IBS patients in both subjective and objective evaluations, and gene expression profiling provided insights into the functional mechanism.

    DOI: 10.1111/jam.13329

  • Japanese traditional dietary fungus koji Aspergillus oryzae functions as a prebiotic for Blautia coccoides through glycosylceramide Japanese dietary fungus koji is a new prebiotic 査読

    Hiroshi Hamajima, Haruka Matsunaga, Ayami Fujikawa, Tomoya Sato, Susumu Mitsutake, Teruyoshi Yanagita, Koji Nagao, Jiro Nakayama, Hiroshi Kitagaki

    SpringerPlus   5 ( 1 )   2016年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Background: The Japanese traditional cuisine, Washoku, considered to be responsible for increased longevity among the Japanese, comprises various foods fermented with the non-pathogenic fungus Aspergillus oryzae (koji). We have recently revealed that koji contains an abundant amount of glycosylceramide. Intestinal microbes have significant effect on health. However, the effects of koji glycosylceramide on intestinal microbes have not been studied. Materials and methods: Glycosylceramide was extracted and purified from koji. C57BL/6N mice were fed a diet containing 1 % purified koji glycosylceramide for 1 week. Nutritional parameters and faecal lipid constituents were analyzed. The intestinal microbial flora of mice on this diet was investigated. Results: Ingested koji glycosylceramide was neither digested by intestinal enzymes nor was it detected in the faeces, suggesting that koji glycosylceramide was digested by the intestinal microbial flora. Intestinal microbial flora that digested koji glycosylceramide had an increased ratio of Blautia coccoides. Stimulation of B. coccoides growth by pure koji glycosylceramide was confirmed in vitro. Conclusions: Koji functions as a prebiotic for B. coccoides through glycosylceramide. Since there are many reports of the effects of B. coccoides on health, an increase in intestinal B. coccoides by koji glycosylceramide might be the connection between Japanese cuisine, intestinal microbial flora, and longevity.

    DOI: 10.1186/s40064-016-2950-6

  • LnqR, a TetR-family transcriptional regulator, positively regulates lacticin Q production in Lactococcus lactis QU 5. 査読 国際誌

    Shun Iwatani, Naoki Ishibashi, Floirendo P Flores, Takeshi Zendo, Jiro Nakayama, Kenji Sonomoto

    FEMS microbiology letters   363 ( 18 )   2016年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Lacticin Q is an unmodified leaderless bacteriocin produced by Lactococcus lactis QU 5. It has been revealed that the production and self-immunity of lacticin Q are facilitated by a gene cluster lnqQBCDEF The gene for a putative TetR-family transcriptional regulator, termed lnqR, was found nearby the lnqQBCDEF cluster, but its involvement in lacticin Q biosynthesis remained unknown. In this study, we created an LnqR-overexpressing QU 5 recombinant by using lactococcal constitutive promoter P32 The recombinant QU 5 showed enhanced production of and self-immunity to lacticin Q. RT-PCR analysis has revealed that an overexpression of LnqR increases the amounts of lnqQBCDEF transcripts, and these six genes are transcribed as an operon in a single transcriptional unit. Interestingly, LnqR expression and thus lacticin Q production by L. lactis QU 5 was found temperature dependent, while LnzR, an LnqR-homologue, in L. lactis QU 14 was expressed in a similar but not identical manner to LnqR, resulting in dissimilar bacteriocin productivities by these strains. This report demonstrates LnqR as the first TetR-family transcriptional regulator involved in LAB bacteriocin biosynthesis and that, as an exceptional case of TetR-family regulators, LnqR positively regulates the transcription of these biosynthetic genes.

    DOI: 10.1093/femsle/fnw200

  • Bacterial community structures of deep-sea water investigated by molecular biological techniques. 査読 国際誌

    Takeshi Terahara, Katsuhisa Yamada, Jiro Nakayama, Yasuhiro Igarashi, Takeshi Kobayashi, Chiaki Imada

    Gene   576 ( 2 Pt 1 )   696 - 700   2016年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    The aim of the present study was to investigate the bacterial community structures of deep-sea water (DSW) and surface seawater (SSW) samples in Japan by molecular biological techniques. DGGE analyses and pyrosequencing analysis revealed that bacterial community structures of DSW were diverse and differed from those of SSW. This is the first report on the horizontal variation of bacterial community structures of DSW throughout Japan. In addition, pyrosequencing analysis revealed that the number of phyla in DSW was larger than that in SSW, and specific phyla, such as Firmicutes and Planctomycetes, were characterized by a higher proportion of the bacterial community structure in DSW than in SSW. Taken together, these results indicate that a variety of bacteria that are specifically adapted to the DSW environments can be expected to be found in DSW, and DSW would thus be a potential resource for novel or unique microorganisms and compounds.

    DOI: 10.1016/j.gene.2015.10.027

  • Functional Analysis of Genes Involved in the Biosynthesis of Enterocin NKR-5-3B, a Novel Circular Bacteriocin. 査読 国際誌

    Rodney H Perez, Naoki Ishibashi, Tomoko Inoue, Kohei Himeno, Yoshimitsu Masuda, Narukiko Sawa, Takeshi Zendo, Pongtep Wilaipun, Vichien Leelawatcharamas, Jiro Nakayama, Kenji Sonomoto

    Journal of bacteriology   198 ( 2 )   291 - 300   2016年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    UNLABELLED: A putative biosynthetic gene cluster of the enterocin NKR-5-3B (Ent53B), a novel circular bacteriocin, was analyzed by sequencing the flanking regions around enkB, the Ent53B structural gene, using a fosmid library. A region approximately 9 kb in length was obtained, and the enkB1, enkB2, enkB3, and enkB4 genes, encoding putative biosynthetic proteins involved in the production, maturation, and secretion of Ent53B, were identified. We also determined the identity of proteins mediating self-immunity against the effects of Ent53B. Heterologous expression systems in various heterologous hosts, such as Enterococcus faecalis and Lactococcus lactis strains, were successfully established. The production and secretion of the mature Ent53B required the cooperative functions of five genes. Ent53B was produced only by those heterologous hosts that expressed protein products of the enkB, enkB1, enkB2, enkB3, and enkB4 genes. Moreover, self-immunity against the antimicrobial action of Ent53B was conferred by at least two independent mechanisms. Heterologous hosts harboring the intact enkB4 gene and/or a combination of intact enkB1 and enkB3 genes were immune to the inhibitory action of Ent53B. IMPORTANCE: In addition to their potential application as food preservatives, circular bacteriocins are now considered possible alternatives to therapeutic antibiotics due to the exceptional stability conferred by their circular structure. The successful practical application of circular bacteriocins will become possible only if the molecular details of their biosynthesis are fully understood. The results of the present study offer a new perspective on the possible mechanism of circular bacteriocin biosynthesis. In addition, since some enterococcal strains are associated with pathogenicity, virulence, and drug resistance, the establishment of the first multigenus host heterologous production of Ent53B has very high practical significance, as it widens the scope of possible Ent53B applications.

    DOI: 10.1128/JB.00692-15

  • In vitro catalytic activity of N-terminal and C-terminal domains in NukM, the post-translational modification enzyme of nukacin ISK-1. 査読

    Chinatsu Shimafuji, Megumi Noguchi, Mami Nishie, Jun-Ichi Nagao, Kouki Shioya, Takeshi Zendo, Jiro Nakayama, Kenji Sonomoto

    Journal of bioscience and bioengineering   120 ( 6 )   624 - 629   2015年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Lantibiotics are antibacterial peptides containing unique thioether cross-links termed lanthionine and methyllanthionine. NukM, the modifying enzyme of nukacin ISK-1, which is produced by Staphylococcus warneri ISK-1, catalyzes the dehydration of specific Ser/Thr residues in a precursor peptide, followed by conjugative addition of intramolecular Cys to dehydrated residues to generate a cyclic structure. By contrast, the precursor peptide of nisin is modified by 2 enzymes, NisB and NisC, which mediate dehydration and cyclization, respectively. While the C-terminal domain of NukM is homologous to NisC, the N-terminal domain has no homology with other known proteins. We expressed and characterized the N- and C-terminal domains of NukM, NukMN, and NukMC, separately. In vitro reconstitution revealed that full-length NukM fully modified the substrate peptide NukA. NukMN partially phosphorylated, dehydrated, and cyclized NukA. By contrast, NukMC did not catalyze dehydration, phosphorylation, or cyclization reactions. Interaction studies using surface plasmon resonance analysis indicated that NukM and NukMN can bind NukA with high affinity, whereas NukMC has low substrate-recognition activity. These results suggest that NukMN is mainly responsible for substrate recognition and dehydration and that the whole NukM structure, including the C-terminal domain, is required for the complete modification of NukA. To the best of our knowledge, this is the first report providing insights into the in vitro catalytic activity of individual domains of a LanM-type modification enzyme.

    DOI: 10.1016/j.jbiosc.2015.03.020

  • Complete genome sequence of Lactobacillus acetotolerans RIB 9124 (NBRC 13120) isolated from putrefied (hiochi) Japanese sake 査読

    Hidehiro Toh, Hidetoshi Morita, Hiroyuki Tsuji, Kazuhiro Iwashita, Nami Goto, Jiro Nakayama, Mitsuo Sekine, Yumiko Kato, Ken Ichiro Suzuki, Nobuyuki Fujita

    Journal of Biotechnology   214   214 - 215   2015年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Lactobacillus acetotolerans RIB 9124 (NBRC 13120) was isolated from putrefied (hiochi) Japanese sake. Here we report the complete genome sequence of this organism. This paper is the first report demonstrating the fully sequenced and completely annotated genome of a L. acetotolerans strain.

    DOI: 10.1016/j.jbiotec.2015.09.006

  • Rationale design of quorum-quenching peptides that target the VirSR system of Clostridium perfringens. 査読 国際誌

    Ravindra Pal Singh, Ken-Ichi Okubo, Kaori Ohtani, Keika Adachi, Kenji Sonomoto, Jiro Nakayama

    FEMS microbiology letters   362 ( 22 )   2015年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    In Clostridium perfringens, a 5-membered thiolactone peptide acts as an autoinducing peptide (AIPCp) to activate the VirSR two-component signal transduction system, which in turn controls the expression of genes encoding multiple toxins, including α, θ and κ. To develop anti-pathogenic agents against virulent C. perfringens, quorum-quenching peptides were rationally designed based on the structure-activity relationship (SAR) data on AIPCp. Alanine scanning study of AIPCp suggested that Trp(3) and Phe(4) are involved in receptor binding and activation, respectively. On the basis of the SAR, we designed two quorum-quenching peptides with different modes of action: Z-AIPCp-L2A/T5A (partial agonist) and Z-AIPCp-F4A/T5S (partial antagonist). Both peptides significantly attenuated transcription of θ toxin gene (pfoA) in a virulent strain of C. perfringens with IC50 = 0.32 and 0.72 μM, respectively.

    DOI: 10.1093/femsle/fnv188

  • Complete genome sequence of Lactobacillus acetotolerans RIB 9124 (NBRC 13120) isolated from putrefied (hiochi) Japanese sake. 査読 国際誌

    Hidehiro Toh, Hidetoshi Morita, Hiroyuki Tsuji, Kazuhiro Iwashita, Nami Goto, Jiro Nakayama, Mitsuo Sekine, Yumiko Kato, Ken-ichiro Suzuki, Nobuyuki Fujita

    Journal of biotechnology   214   214 - 5   2015年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Lactobacillus acetotolerans RIB 9124 (NBRC 13120) was isolated from putrefied (hiochi) Japanese sake. Here we report the complete genome sequence of this organism. This paper is the first report demonstrating the fully sequenced and completely annotated genome of a L. acetotolerans strain.

    DOI: 10.1016/j.jbiotec.2015.09.006

  • Arthroamide, a Cyclic Depsipeptide with Quorum Sensing Inhibitory Activity from Arthrobacter sp. 査読 国際誌

    Yasuhiro Igarashi, Kazuki Yamamoto, Takao Fukuda, Akane Shojima, Jiro Nakayama, Lorena Carro, Martha E Trujillo

    Journal of natural products   78 ( 11 )   2827 - 2831   2015年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Nonfilamentous actinobacteria have been less studied as secondary metabolite producers than their filamentous counterparts such as Streptomyces. From our collection of nonfilamentous actinobacteria isolated from sandstone, an Arthrobacter strain was found to produce a new cyclic peptide arthroamide (1) together with the known compound turnagainolide A (2). These compounds inhibited the quorum sensing signaling of Staphylococcus aureus in the submicromolar to micromolar range.

    DOI: 10.1021/acs.jnatprod.5b00540

  • Identification, Characterization, and Three-Dimensional Structure of the Novel Circular Bacteriocin, Enterocin NKR-5-3B, from Enterococcus faecium. 査読 国際誌

    Kohei Himeno, K Johan Rosengren, Tomoko Inoue, Rodney H Perez, Michelle L Colgrave, Han Siean Lee, Lai Y Chan, Sónia Troeira Henriques, Koji Fujita, Naoki Ishibashi, Takeshi Zendo, Pongtep Wilaipun, Jiro Nakayama, Vichien Leelawatcharamas, Hiroyuki Jikuya, David J Craik, Kenji Sonomoto

    Biochemistry   54 ( 31 )   4863 - 4876   2015年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Enterocin NKR-5-3B, one of the multiple bacteriocins produced by Enterococcus faecium NKR-5-3, is a 64-amino acid novel circular bacteriocin that displays broad-spectrum antimicrobial activity. Here we report the identification, characterization, and three-dimensional nuclear magnetic resonance solution structure determination of enterocin NKR-5-3B. Enterocin NKR-5-3B is characterized by four helical segments that enclose a compact hydrophobic core, which together with its circular backbone impart high stability and structural integrity. We also report the corresponding structural gene, enkB, that encodes an 87-amino acid precursor peptide that undergoes a yet to be described enzymatic processing that involves adjacent cleavage and ligation of Leu(24) and Trp(87) to yield the mature (circular) enterocin NKR-5-3B.

    DOI: 10.1021/acs.biochem.5b00196

  • Cyclodepsipeptides produced by actinomycetes inhibit cyclic-peptide-mediated quorum sensing in Gram-positive bacteria. 査読 国際誌

    Said E Desouky, Akane Shojima, Ravindra Pal Singh, Takahisa Matsufuji, Yasuhiro Igarashi, Takashi Suzuki, Tohru Yamagaki, Ken-Ichi Okubo, Kaori Ohtani, Kenji Sonomoto, Jiro Nakayama

    FEMS microbiology letters   362 ( 14 )   2015年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Cyclic peptides are commonly used as quorum-sensing autoinducers in Gram-positive Firmicutes bacteria. Well-studied examples of such molecules are thiolactone and lactone, used to regulate the expression of a series of virulence genes in the agr system of Staphylococcus aureus and the fsr system of Enterococcus faecalis, respectively. Three cyclodepsipeptides WS9326A, WS9326B and cochinmicin II/III were identified as a result of screening actinomycetes culture extracts for activity against the agr/fsr system. These molecules are already known as receptor antagonists, the first two for tachykinin and the last one for endothelin. WS9326A also inhibited the transcription of pfoA regulated by the VirSR two-component system in Clostridium perfringens. Receptor-binding assays using a fluorescence-labeled autoinducer (FITC-GBAP) showed that WS9326A and WS9326B act as receptor antagonists in this system. In addition, an ex vivo assay showed that WS9326B substantially attenuated the toxicity of S. aureus for human corneal epithelial cells. These results suggest that these three natural cyclodepsipeptides have therapeutic potential for targeting the cyclic peptide-mediated quorum sensing of Gram-positive pathogens.

    DOI: 10.1093/femsle/fnv109

  • Development of quorum-sensing inhibitors targeting the fsr system of enterococcus faecalis 査読

    Ravindra Pal Singh, Jiro Nakayama

    Quorum Sensing vs Quorum Quenching: A Battle with no end in Sight   319 - 326   2015年1月

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    記述言語:英語  

    Enterococcus spp. can cause illnesses such as bacteremia, endocarditis, urinary tract infections, posttreatment endophthalmitis, and endodontic infections (Murray 2000; Marothi et al. 2005). Medical treatment of these infectious diseases depends largely on bactericidal or bacteriostatic antibiotics. However, frequent use of such antibiotics has led to the development of drug-resistant bacterial strains, which are difficult to treat (Marothi et al. 2005; Murray 2000). As a result, the blockage of bacterial quorum-sensing (QS) systems has attracted attention owing to its potential to attenuate bacterial virulence without inducing bactericidal pressures that lead to drug resistance. This approach, called quorum quenching (QQ), can be undertaken partially or completely independently of antibiotic treatment. Among several QQ strategies, the use of QS inhibitors (QSIs), which are small molecules that have no adverse effects on bacteria, offers advantages in terms of drug delivery and decreased damage to commensal microbiota.

    DOI: 10.1007/978-81-322-1982-8__25

  • Development of quorum-sensing inhibitors targeting the fsr system of enterococcus faecalis 査読

    Ravindra Pal Singh, Jiro Nakayama

    Quorum Sensing vs Quorum Quenching: A Battle with no end in Sight   319 - 326   2015年1月

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    記述言語:英語  

    Enterococcus spp. can cause illnesses such as bacteremia, endocarditis, urinary tract infections, posttreatment endophthalmitis, and endodontic infections (Murray 2000; Marothi et al. 2005). Medical treatment of these infectious diseases depends largely on bactericidal or bacteriostatic antibiotics. However, frequent use of such antibiotics has led to the development of drug-resistant bacterial strains, which are difficult to treat (Marothi et al. 2005; Murray 2000). As a result, the blockage of bacterial quorum-sensing (QS) systems has attracted attention owing to its potential to attenuate bacterial virulence without inducing bactericidal pressures that lead to drug resistance. This approach, called quorum quenching (QQ), can be undertaken partially or completely independently of antibiotic treatment. Among several QQ strategies, the use of QS inhibitors (QSIs), which are small molecules that have no adverse effects on bacteria, offers advantages in terms of drug delivery and decreased damage to commensal microbiota.

    DOI: 10.1007/978-81-322-1982-8__25

  • Quorum-sensing systems in enterococci 査読

    Ravindra Pal Singh, Jiro Nakayama

    Quorum Sensing vs Quorum Quenching: A Battle with no end in Sight   155 - 163   2015年1月

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    記述言語:英語  

    Enterococcus is a genus of Gram-positive bacteria that is ubiquitous in natural ecosystems, plants, and animals. Some species of Enterococcus are present in the normal gastrointestinal bacterial community. However, others notably Enterococcus faecalis and Enterococcus faecium often cause opportunistic infections such as bacteremia, endocarditis, urinary tract infections, posttreatment endodontic infections, and endophthalmitis (Arias et al. 2010). Bacteria often use quorum sensing (QS) systems to control the expression of certain virulence genes and establish infection efficiently (Waters and Bassler 2005).

    DOI: 10.1007/978-81-322-1982-8__14

  • Effects of bifidobacterial supplementation to pregnant women and infants in the prevention of allergy development in infants and on fecal microbiota. 査読 国際誌

    Tadao Enomoto, Masanori Sowa, Keiji Nishimori, Shinichiro Shimazu, Akira Yoshida, Kazuko Yamada, Fukumi Furukawa, Takemasa Nakagawa, Naotake Yanagisawa, Noriyuki Iwabuchi, Toshitaka Odamaki, Fumiaki Abe, Jiro Nakayama, Jin-Zhong Xiao

    Allergology international : official journal of the Japanese Society of Allergology   63 ( 4 )   575 - 85   2014年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    BACKGROUND: Probiotic administration may be a useful method for preventing allergies in infants; however, there have been controversial results about the efficacy. We investigated the effects of bifidobacterial supplementation on the risk of developing allergic diseases in the Japanese population. METHODS: In an open trial, we gave Bifidobacterium breve M-16V and Bifidobacterium longum BB536 prenatally to 130 mothers beginning 1 month prior to delivery and postnatally to their infants for 6 months. Another 36 mother-infant pairs served as controls and did not receive the bifidobacterial supplementation. Development of allergic symptoms in the infants was assessed at 4, 10 and 18 months of age. Fecal samples were collected from the mothers and infants. RESULTS: The risk of developing eczema/atopic dermatitis (AD) during the first 18 months of life was significantly reduced in infants in the probiotic group (OR: 0.231 [95% CI: 0.084-0.628] and 0.304 [0.105-0.892] at 10 and 18 months of age, respectively). Pyrosequencing analyses indicated an altered composition of the fecal microbiota at 4 months for infants who developed eczema/AD at 4 and 10 months of age. The proportion of Proteobacteria was significantly lower (P = 0.007) in mothers at the time of delivery who received the supplementation when compared with the control group and was positively correlated (r = 0.283, P = 0.024) with that of infants at 4 months of age. No adverse effects were related to the use of probiotics. CONCLUSIONS: These data suggest that the prenatal and postnatal supplementation of bifidobacteria is effective in primary preventing allergic diseases. Some limited changes in the composition of fecal microbiota by the bifidobacterial supplementation were observed.

    DOI: 10.2332/allergolint.13-OA-0683

  • Gene cluster responsible for secretion of and immunity to multiple bacteriocins, the NKR-5-3 enterocins. 査読 国際誌

    Naoki Ishibashi, Kohei Himeno, Yoshimitsu Masuda, Rodney Honrada Perez, Shun Iwatani, Takeshi Zendo, Pongtep Wilaipun, Vichien Leelawatcharamas, Jiro Nakayama, Kenji Sonomoto

    Applied and environmental microbiology   80 ( 21 )   6647 - 55   2014年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Enterococcus faecium NKR-5-3, isolated from Thai fermented fish, is characterized by the unique ability to produce five bacteriocins, namely, enterocins NKR-5-3A, -B, -C, -D, and -Z (Ent53A, Ent53B, Ent53C, Ent53D, and Ent53Z). Genetic analysis with a genome library revealed that the bacteriocin structural genes (enkA [ent53A], enkC [ent53C], enkD [ent53D], and enkZ [ent53Z]) that encode these peptides (except for Ent53B) are located in close proximity to each other. This NKR-5-3ACDZ (Ent53ACDZ) enterocin gene cluster (approximately 13 kb long) includes certain bacteriocin biosynthetic genes such as an ABC transporter gene (enkT), two immunity genes (enkIaz and enkIc), a response regulator (enkR), and a histidine protein kinase (enkK). Heterologous-expression studies of enkT and ΔenkT mutant strains showed that enkT is responsible for the secretion of Ent53A, Ent53C, Ent53D, and Ent53Z, suggesting that EnkT is a wide-range ABC transporter that contributes to the effective production of these bacteriocins. In addition, EnkIaz and EnkIc were found to confer self-immunity to the respective bacteriocins. Furthermore, bacteriocin induction assays performed with the ΔenkRK mutant strain showed that EnkR and EnkK are regulatory proteins responsible for bacteriocin production and that, together with Ent53D, they constitute a three-component regulatory system. Thus, the Ent53ACDZ gene cluster is essential for the biosynthesis and regulation of NKR-5-3 enterocins, and this is, to our knowledge, the first report that demonstrates the secretion of multiple bacteriocins by an ABC transporter.

    DOI: 10.1128/AEM.02312-14

  • Monitoring of the microbiota profile in nukadoko, a naturally fermented rice bran bed for pickling vegetables. 査読

    Hiroshi Ono, Shoko Nishio, Jun Tsurii, Tetsuhiro Kawamoto, Kenji Sonomoto, Jiro Nakayama

    Journal of bioscience and bioengineering   118 ( 5 )   520 - 5   2014年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Nukadoko is a fermented rice bran mash traditionally used for pickling vegetables in Japan. To date, the production of both homemade and commercial nukadoko depends on natural fermentation without using starter cultures. Here, we monitored chemical and microbiological changes in the initial batch fermentation of nukadoko. Nukadoko samples were prepared by spontaneous fermentation of four different brands of rice bran, and microbiome dynamics were analyzed for 2 months. In the first week, non-Lactobacillales lactic acid bacteria (LAB) species, which differed among the samples, grew proportionally to pH decrease and lactate increase. Thereafter, Lactobacillus plantarum started growing and consumed residual sugars, causing further lactate increase in nukadoko. Finally, microbial communities in all tested nukadoko samples were dominated by L. plantarum. Taken together, our results suggest that the mixture of the fast-growing LAB species and slow-growing L. plantarum may be used as a suitable starter culture to promote the initial fermentation of nukadoko.

    DOI: 10.1016/j.jbiosc.2014.04.017

  • Effects of Japanese pepper and red pepper on the microbial community during nukadoko fermentation 査読 国際誌

    H. Ono, S. Nishio, J. Tsurii, T. Kawamoto, K. Sonomoto and J. Nakayama

    Biosci Microbiota Food Health   34 ( 1 )   2014年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: doi: 10.12938/bmfh

  • Senior Thai fecal microbiota comparison between vegetarians and non-vegetarians using PCR-DGGE and real-time PCR. 査読 国際誌

    Supatjaree Ruengsomwong, Yuki Korenori, Naoshige Sakamoto, Bhusita Wannissorn, Jiro Nakayama, Sunee Nitisinprasert

    Journal of microbiology and biotechnology   24 ( 8 )   1026 - 33   2014年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    The fecal microbiotas were investigated in 13 healthy Thai subjects using polymerase chain reaction denaturing gradient gel electrophoresis (PCR-DGGE). Among the 186 DNA bands detected on the polyacrylamide gel, 37 bands were identified as representing 11 species: Bacteroides thetaiotaomicron, Bacteroides ovatus, Bacteroides uniformis, Bacteroides vulgatus, Clostridium colicanis, Eubacterium eligenes, E. rectale, Faecalibacterium prausnitzii, Megamonas funiformis, Prevotella copri, and Roseburia intestinalis, belonging mainly to the groups of Bacteroides, Prevotella, Clostridium, and F. prausnitzii. A dendrogram of the PCR-DGGE divided the subjects; vegetarians and non-vegetarians. The fecal microbiotas were also analyzed using a quantitative real-time PCR focused on Bacteroides, Bifidobacterium, Enterobacteriaceae, Clostrium coccoides-Eubacterium rectale, C. leptum, Lactobacillus, and Prevotella. The nonvegetarian and vegetarian subjects were found to have significant differences in the high abundance of the Bacteroides and Prevotella genera, respectively. No significant differences were found in the counts of Bifidabacterium, Enterobacteriaceae, C. coccoides-E. rectale group, C. leptum group, and Lactobacillus. Therefore, these findings on the microbiota of healthy Thais consuming different diets could provide helpful data for predicting the health of South East Asians with similar diets.

    DOI: 10.4014/jmb.1310.10043

  • Biological function of a DUF95 superfamily protein involved in the biosynthesis of a circular bacteriocin, leucocyclicin Q. 査読

    Fuqin Mu, Yoshimitsu Masuda, Takeshi Zendo, Hiroshi Ono, Hiroshi Kitagawa, Haruo Ito, Jiro Nakayama, Kenji Sonomoto

    Journal of bioscience and bioengineering   117 ( 2 )   158 - 164   2014年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Biological functions of a DUF95 superfamily protein in the biosynthesis gene cluster of a novel circular bacteriocin, leucocyclicin Q (LcyQ), were characterized in this paper. Sequence analysis and database search of the regions flanking the LcyQ structural gene lcyQ revealed four open reading frames (lcyR, lcyB, lcyC, and lcyD) related to bacteriocin biosynthesis. LcyD shares some similarity to the DUF95 superfamily proteins, often found in the biosynthetic gene clusters of circular bacteriocins. Mass spectrometry analysis showed accumulation of active mature LcyQ inside lcyD knockout cells. Heterologous expression of lcyD demonstrated that it confers robust immunity against LcyQ. Peptide release/binding assay revealed that the immunity could be attributed to the secretion of LcyQ to the cell exterior. Thus, the DUF95 superfamily protein has a dual function in the biosynthesis of LcyQ, as an immunity-associated transporter and as a secretion-aiding agent. Accumulation of mature LcyQ inside the cell in lcyD knockout strains, further implied that cyclization occurs within the cell. To the best of our knowledge, this is the first report on LcyQ cyclization inside the cell and the dual role of a DUF95 superfamily protein in circular bacteriocin biosynthesis.

    DOI: 10.1016/j.jbiosc.2013.06.023

  • Draft Genome Sequence of Lactobacillus namurensis Chizuka 01, Isolated from Nukadoko, a Pickling Bed of Fermented Rice Bran. 査読 国際誌

    Keita Kato, Hidehiro Toh, Naoshige Sakamoto, Kazuki Mori, Kosuke Tashiro, Naruhiro Hibi, Kenji Sonomoto, Jiro Nakayama

    Genome announcements   2 ( 1 )   2014年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Lactobacillus namurensis Chizuka 01 was isolated from nukadoko, which is a fermented rice bran bed traditionally used in Japan for pickling vegetables. Here, we report the first draft of an annotated genome sequence of this organism. This paper is the first published report of the genomic sequence of L. namurensis.

    DOI: 10.1128/genomeA.01263-13

  • Bactericidal activity of nukacin ISK-1 An alternative mode of action 査読

    Urmi Roy, Mohammad Riazul Islam, Jun Ichi Nagao, Hiroshi Iida, Abdullah Al Mahin, Mengqi Li, Takeshi Zendo, Jiro Nakayama, Kenji Sonomoto

    Bioscience, Biotechnology and Biochemistry   78 ( 7 )   1270 - 1273   2014年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    We previously reported bacteriostatic action of nukacin ISK-1 against Bacillus subtilis JCM 1465T. Here, we found its bactericidal activity against Micrococcus luteus DSM 1790 and Staphylococcus simulans 22, showing decrease in cell viability, cell lysis, and dissipation of the membrane potential. Moreover, leakage of small molecules such as K+, suggested the formation of small-sized or specific K+-conducting-pores by nukacin ISK-1.

    DOI: 10.1080/09168451.2014.918485

  • Transcriptomic study reveals new pathways and genes involved in Enterococcus faecalis V583 response to a therapeutic dose of vancomycin 査読

    Tânia Ribeiro, Neuza Teixeira, Ryoji Yokohata, Jiro Nakayama, Michael S. Gilmore, Maria de Fátima S Lopes

    Archives of Clinical Microbiology   5 ( 1 )   2014年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Background: An enterococcal strain carrying the VanB resistance type can become susceptible if impaired in other genes unrelated to the vanB operon. This fact alone illustrates the lack of knowledge on the vancomycin mode of action. This antibiotic is still usable to treat serious infections caused by multiresistant enterococcal strains, but may not be so for long. This work was thus set up to gather a body of knowledge that can be used in the future to increase efficacy against both vancomycin resistant (VRE) and susceptible enterococci (VSE). Methods and Findings: Microarrays were used to detect the genetic response of the VanB carrying strain Enterococcus faecalis V583 to a therapeutic dose (10 mg/ml) of vancomycin. Besides the vanRS genes, two other two-component systems were induced. The therapeutic dose of vancomycin was found to act as an anti-virulence agent, by turning-off the Fsr quorum-sensing system. Key regulators and metabolic enzymes, involved in trafficking carbon sources into glycolysis and isoprenoid synthesis and utilization, were also affected in order to support cell-wall synthesis. Also, cell-wall modification involving lipotheicoic acid synthesis, DNA repair and protein folding were highly responsive functions to the vancomycin dose tested. Conclusions: Overall, our results provide clues on the ability of a VRE strain to stand vancomycin and on the mode of action of the antibiotic. VRE response to a vancomycin therapeutic dose involves an intricate regulatory network and metabolic adjustment which is worth solving as it can help finding new targets to fight both VRE and VSE infections.

    DOI: 10.3823/277

  • Gene cluster responsible for secretion of and immunity to multiple bacteriocins, the NKR-5-3 enterocins 査読

    Naoki Ishibashi, Kohei Himeno, Yoshimitsu Masuda, Rodney Honrada Perez, Shun Iwatani, Takeshi Zendo, Pongtep Wilaipun, Vichien Leelawatcharamas, Jiro Nakayama, Kenji Sonomoto

    Applied and Environmental Microbiology   80 ( 21 )   6647 - 6655   2014年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Enterococcus faecium NKR-5-3, isolated from Thai fermented fish, is characterized by the unique ability to produce five bacteriocins, namely, enterocins NKR-5-3A, -B, -C, -D, and -Z (Ent53A, Ent53B, Ent53C, Ent53D, and Ent53Z). Genetic analysis with a genome library revealed that the bacteriocin structural genes (enkA [ent53A], enkC [ent53C], enkD [ent53D], and enkZ [ent53Z]) that encode these peptides (except for Ent53B) are located in close proximity to each other. This NKR-5-3ACDZ (Ent53ACDZ) enterocin gene cluster (approximately 13 kb long) includes certain bacteriocin biosynthetic genes such as an ABC transporter gene (enkT), two immunity genes (enkIaz and enkIc), a response regulator (enkR), and a histidine protein kinase (enkK). Heterologous- expression studies of enkT and ΔenkT mutant strains showed that enkT is responsible for the secretion of Ent53A, Ent53C, Ent53D, and Ent53Z, suggesting that EnkT is a wide-range ABC transporter that contributes to the effective production of these bacteriocins. In addition, EnkIaz and EnkIc were found to confer self-immunity to the respective bacteriocins. Furthermore, bacteriocin induction assays performed with the ΔenkRK mutant strain showed that EnkR and EnkK are regulatory proteins responsible for bacteriocin production and that, together with Ent53D, they constitute a three-component regulatory system. Thus, the Ent53ACDZ gene cluster is essential for the biosynthesis and regulation of NKR-5-3 enterocins, and this is, to our knowledge, the first report that demonstrates the secretion of multiple bacteriocins by an ABC transporter.

    DOI: 10.1128/AEM.02312-14

  • Effects of bifidobacterial supplementation to pregnant women and infants in the prevention of allergy development in infants and on fecal microbiota 査読

    Tadao Enomoto, Masanori Sowa, Keiji Nishimori, Shinichiro Shimazu, Akira Yoshida, Kazuko Yamada, Fukumi Furukawa, Takemasa Nakagawa, Naotake Yanagisawa, Noriyuki Iwabuchi, Toshitaka Odamaki, Fumiaki Abe, Jiro Nakayama, Jin Zhong Xiao

    Allergology International   63 ( 4 )   575 - 585   2014年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Background: Probiotic administration may be a useful method for preventing allergies in infants; however, there have been controversial results about the efficacy. We investigated the effects of bifidobacterial supplementation on the risk of developing allergic diseases in the Japanese population.
    Methods: In an open trial, we gave Bifidobacterium breve M-16V and Bifidobacterium longum BB536 prenatally to 130 mothers beginning 1 month prior to delivery and postnatally to their infants for 6 months. Another 36 mother-infant pairs served as controls and did not receive the bifidobacterial supplementation. Development of allergic symptoms in the infants was assessed at 4, 10 and 18 months of age. Fecal samples were collected from the mothers and infants.
    Results: The risk of developing eczema[1]atopic dermatitis (AD) during the first 18 months of life was significantly reduced in infants in the probiotic group (OR: 0.231 [95% CI: 0.084-0.628] and 0.304 [0.105-0.892] at 10 and 18 months of age, respectively). Pyrosequencing analyses indicated an altered composition of the fecal microbiota at 4 months for infants who developed eczema[1]AD at 4 and 10 months of age. The proportion of Proteobacteria was significantly lower (P = 0.007) in mothers at the time of delivery who received the supplementation when compared with the control group and was positively correlated (r = 0.283, P = 0.024) with that of infants at 4 months of age. No adverse effects were related to the use of probiotics.
    Conclusions: These data suggest that the prenatal and postnatal supplementation of bifidobacteria is effective in primary preventing allergic diseases. Some limited changes in the composition of fecal microbiota by the bifidobacterial supplementation were observed.

    DOI: 10.2332/allergolint.13-OA-0683

  • Lethal hydroxyl radical accumulation by a lactococcal bacteriocin, lacticin Q. 査読 国際誌

    Mengqi Li, Fuminori Yoneyama, Nayu Toshimitsu, Takeshi Zendo, Jiro Nakayama, Kenji Sonomoto

    Antimicrobial agents and chemotherapy   57 ( 8 )   3897 - 3902   2013年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    The antimicrobial mechanism of a lactococcal bacteriocin, lacticin Q, can be described by the toroidal pore model without any receptor. However, lacticin Q showed different degrees of activity (selective antimicrobial activity) against Gram-positive bacteria even among related species. The ability of lacticin Q to induce pore formation in liposomes composed of lipids from different indicator strains indicated that its selective antimicrobial activity could not be attributed only to membrane lipid composition. We investigated the accumulation of deleterious hydroxyl radicals after exposure to lacticin Q as a contributing factor to cell death in the indicator strains. When lacticin Q of the same concentration as the MIC or minimum bactericidal concentration was added to the indicator cultures, high levels of hydroxyl radical accumulation were detected. Treatment with hydroxyl radical scavengers, thiourea and 2,2'-bipyridyl, decreased the levels of hydroxyl radical accumulation and recovered cell viability. These results suggest that, with or without pore formation, the final antimicrobial mechanism of lacticin Q is the accumulation of hydroxyl radicals, which varies by strain, resulting in the selective antimicrobial activity of lacticin Q.

    DOI: 10.1128/AAC.00638-13

  • Drosophila Host Model Reveals New Enterococcus faecalis Quorum-Sensing Associated Virulence Factors 査読

    Neuza Teixeira, Sriram Varahan, Matthew J. Gorman, Kelli L. Palmer, Anna Zaidman-Remy, Ryoji Yokohata, Jiro Nakayama, Lynn E. Hancock, António Jacinto, Michael S. Gilmore, Maria de Fátima Silva Lopes

    PLoS One   8 ( 5 )   2013年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Enterococcus faecalis V583 is a vancomycin-resistant clinical isolate which belongs to the hospital-adapted clade, CC2. This strain harbours several factors that have been associated with virulence, including the fsr quorum-sensing regulatory system that is known to control the expression of GelE and SprE proteases. To discriminate between genes directly regulated by Fsr, and those indirectly regulated as the result of protease expression or activity, we compared gene expression in isogenic mutants of V583 variously defective in either Fsr quorum sensing or protease expression. Quorum sensing was artificially induced by addition of the quorum signal, GBAP, exogenously in a controlled manner. The Fsr regulon was found to be restricted to five genes, gelE, sprE, ef1097, ef1351 and ef1352. Twelve additional genes were found to be dependent on the presence of GBAP-induced proteases. Induction of GelE and SprE by GBAP via Fsr resulted in accumulation of mRNA encoding lrgAB, and this induction was found to be lytRS dependent. Drosophila infection was used to discern varying levels of toxicity stemming from mutations in the fsr quorum regulatory system and the genes that it regulates, highlighting the contribution of LrgAB and bacteriocin EF1097 to infection toxicity. A contribution of SprE to infection toxicity was also detected. This work brought to light new players in E. faecalis success as a pathogen and paves the way for future studies on host tolerance mechanisms to infections caused by this important nosocomial pathogen.

    DOI: 10.1371/journal.pone.0064740

  • Up to species-level community analysis of human gut microbiota by 16S rRNA amplicon pyrosequencing 査読 国際誌

    Nakayama, J., Jiang, J., Watanabe, K., Chen, K., Ninxin, H., Matsuda, K., Kurakawa, T., Tsuji, H., Sonomoto, K., and Lee, Y.-K.

    Bioscience, Microflora, Food, Health   32   2013年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • Bifunctional gene cluster lnqBCDEF mediates bacteriocin production and immunity with differential genetic requirements. 査読 国際誌

    Shun Iwatani, Yuko Horikiri, Takeshi Zendo, Jiro Nakayama, Kenji Sonomoto

    Applied and environmental microbiology   79 ( 7 )   2446 - 2449   2013年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    A comprehensive gene disruption of lacticin Q biosynthetic cluster lnqQBCDEF was carried out. The results demonstrated the necessity of the complete set of lnqQBCDEF for lacticin Q production, whereas immunity was flexible, with LnqEF (ABC transporter) being essential for and LnqBCD partially contributing to immunity.

    DOI: 10.1128/AEM.03783-12

  • Interactions of the intact FsrC membrane histidine kinase with the tricyclic peptide inhibitor siamycin I revealed through synchrotron radiation circular dichroism. 査読 国際誌

    Mary K Phillips-Jones, Simon G Patching, Shalini Edara, Jiro Nakayama, Rohanah Hussain, Giuliano Siligardi

    Physical chemistry chemical physics : PCCP   15 ( 2 )   444 - 447   2013年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    The suitability of synchrotron radiation circular dichroism spectroscopy (SRCD) for studying interactions between the tricyclic peptide inhibitor siamycin I and the intact FsrC membrane sensor kinase in detergent micelles has been established. In the present study, tertiary structural changes demonstrate that inhibitor binding occurs at a different, non-overlapping site to the native ligand, GBAP.

    DOI: 10.1039/c2cp43722h

  • Identification of the genes involved in the secretion and self-immunity of lacticin Q, an unmodified leaderless bacteriocin from Lactococcus lactis QU 5. 査読 国際誌

    Shun Iwatani, Fuminori Yoneyama, Shiho Miyashita, Takeshi Zendo, Jiro Nakayama, Kenji Sonomoto

    Microbiology (Reading, England)   158 ( 12 )   2927 - 2935   2012年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Lacticin Q (LnqQ) produced by Lactococcus lactis QU 5 is an unmodified linear bacteriocin, which is synthesized without an N-terminal leader peptide. In vitro synthesis and in vivo expression of LnqQ have revealed the intracellular toxicity of this leaderless peptide, as well as the necessity of a dedicated secretion and self-immunity system of producer cells. Further DNA sequencing and analysis have discovered 11 putative orf genes at the LnqQ locus. None of the orf genes showed similarities to any of the bacteriocin biosynthetic genes characterized to date; however, six orf genes (orf2q-7q), not including the structural gene (lnqQ), were highly conserved at the lacticin Z locus (orf2z-7z), which is a LnqQ homologue produced by L. lactis QU 14. ORF2q (ORF2z), the gene of which is located upstream of the structural gene, is a putative transcriptional regulator, whereas ORF6q and ORF7q (ORF6z and ORF7z) form a putative ATP-binding cassette transporter. The ORF3q-5q (ORF3z-5z) are all predicted to be membrane proteins with no clear functions. Co-expression of LnqQ and ORF3q-7q in a heterologous host allowed the extracellular production of LnqQ; additionally, the expression of ORF3q-7q rendered the host cells immune to LnqQ. This self-immunity was facilitated possibly by two means; firstly, by secreting the active LnqQ peptides, thus reducing the intracellular toxicity, and secondly, by protecting the host cells from extracellularly released LnqQ. This is the first report, to our knowledge, that describes intracellular toxicity of a leaderless bacteriocin and provides a rare example of biosynthetic genes that are required for bacteriocin secretion and immunity.

    DOI: 10.1099/mic.0.062943-0

  • Monitoring of the multiple bacteriocin production by Enterococcus faecium NKR-5-3 through a developed liquid chromatography and mass spectrometry-based quantification system. 査読

    Rodney H Perez, Kohei Himeno, Naoki Ishibashi, Yoshimitsu Masuda, Takeshi Zendo, Koji Fujita, Pongtep Wilaipun, Vichien Leelawatcharamas, Jiro Nakayama, Kenji Sonomoto

    Journal of bioscience and bioengineering   114 ( 5 )   490 - 496   2012年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Enterococcus faecium NKR-5-3 produces four antimicrobial peptides referred here as enterocins NKR-5-3A, B, C and D. A two-step electrospray ionization-liquid chromatography and mass spectrometry (ESI-LC/MS)-based quantification system was developed to monitor its multiple bacteriocin production profiles, which is essential in understanding the complex production regulation mechanism of strain NKR-5-3. The developed ESI-LC/MS-based quantification system can easily monitor the multiple bacteriocin production of this strain. Using the developed system, the production of enterocin NKR-5-3B was found to be not as variable as those of the other enterocins in different cultivation media. Production of enterocin NKR-5-3B was also found to have a wider optimum incubation temperature (20-30°C) than enterocins NKR-5-3A, C and D (25°C). Furthermore, at least 2 nM of the bacteriocin-like inducing peptide, enterocin NKR-5-3D, regulated the production of NKR-5-3 enterocins except enterocin NKR-5-3B. These findings taken together suggest that enterocin NKR-5-3B has an independent production regulation mechanism from the other NKR-5-3 enterocins. The developed system could effectively pin-point the production profiles of the multiple bacteriocins of E. faecium NKR-5-3 under different fermentation conditions.

    DOI: 10.1016/j.jbiosc.2012.06.003

  • Interactions of the intact FsrC membrane histidine kinase with its pheromone ligand GBAP revealed through synchrotron radiation circular dichroism 査読

    Simon G. Patching, Shalini Edara, Pikyee Ma, Jiro Nakayama, Rohanah Hussain, Giuliano Siligardi, Mary K. Phillips-Jones

    Biochimica et Biophysica Acta - Biomembranes   1818 ( 7 )   1595 - 1602   2012年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    FsrC is the membrane-bound histidine kinase component of the Fsr two-component signal transduction system involved in quorum sensing in the hospital-acquired infection agent Enterococcus faecalis. Synchrotron radiation circular dichroism spectroscopy was used here to study the intact purified protein solubilised in detergent micelles. Conditions required for FsrC stability in detergent were firstly determined and tested by prolonged exposure of stabilised protein to far-ultraviolet radiation. Using stabilised purified protein, far-ultraviolet synchrotron radiation circular dichroism revealed that FsrC is 61% α-helical and that it is relatively thermostable, retaining at least 57% secondary structural integrity at 90 °C in the presence or absence of gelatinase biosynthesis-activating pheromone (GBAP). Whilst binding of the quorum pheromone ligand GBAP did not significantly affect FsrC secondary structure, near-ultraviolet spectra revealed that the tertiary structure in the regions of the Tyr and Trp residues was significantly affected. Titration experiments revealed a calculated k value of 2 μM indicative of relatively loose binding of gelatinase biosynthesis-activating pheromone to FsrC. Although use of synchrotron radiation circular dichroism has been applied to membrane proteins previously, to our knowledge this is the first report of its use to determine a k value for an intact membrane protein. Based on our findings, we suggest that synchrotron radiation circular dichroism will be a valuable technique for characterising ligand binding by other membrane sensor kinases and indeed other membrane proteins in general. It further provides a valuable screening tool for membrane protein stability under a range of detergent conditions prior to downstream structural methods such as crystallisation and NMR experiments particularly when lower detergent concentrations are used. Crown Copyright © 2012 Published by Elsevier B.V. All rights reserved. d d

    DOI: 10.1016/j.bbamem.2012.02.015

  • Identification of enterocin NKR-5-3C, a novel class IIa bacteriocin produced by a multiple bacteriocin producer, Enterococcus faecium NKR-5-3 査読

    Kohei Himeno, Koji Fujita, Takeshi Zendo, Pongtep Wilaipun, Naoki Ishibashi, Yoshimitsu Masuda, Fuminori Yoneyama, Vichien Leelawatcharamas, Jiro Nakayama, Kenji Sonomoto

    Bioscience, Biotechnology and Biochemistry   76 ( 6 )   1245 - 1247   2012年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    The structure of enterocin NKR-5-3C, an antilisterial bacteriocin produced by a multiple bacteriocin producer, Enterococcus faecium NKR-5-3, was determined. Enterocin NKR-5-3C is a novel class IIa bacteriocin that possesses an YGNGL motif sequence and two disulfide bridges in its structure. It is encoded on gene ent53C together with an 18-amino-acid-residue double glycine leader peptide.

    DOI: 10.1271/bbb.120089

  • Purification and characterization of multiple bacteriocins and an inducing peptide produced by Enterococcus faecium NKR-5-3 from Thai fermented fish 査読

    Naoki Ishibashi, Kohei Himeno, Koji Fujita, Yoshimitsu Masuda, Rodney Honrada Perez, Takeshi Zendo, Pongtep Wilaipun, Vichien Leelawatcharamas, Jiro Nakayama, Kenji Sonomoto

    Bioscience, Biotechnology and Biochemistry   76 ( 5 )   947 - 953   2012年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Enterocins NKR-5-3A, B, C, and D were purified from the culture supernatant of Enterococcus faecium NKR-5-3 and characterized. Among the four purified peptides, enterocin NKR-5-3A (5242.3 Da) was identical to brochocin A, produced by Brochothrix campestris ATCC 43754, in mature peptides, and its putative synergistic peptide, enterocin NKR-5-3Z, was found to be encoded in ent53Z downstream of ent53A, encoding enterocin NKR-5-3A. Enterocin NKR-5-3B (6316.4Da) showed a broad antimicrobial spectrum, and enterocin NKR-5-3C (4512.8 Da) showed high activity against Listeria. Enterocin NKR-5-3D (2843.5 Da), showing high homology to an inducing peptide produced by Lactobacillus sakei 5, induced the production of the enterocins. The enterocins showed different antimicrobial spectra and intensities. E. faecium NKR-5-3 concomitantly produced enterocins NKR-5-3A, B, C, and D which probably belong to different classes of bacteriocins. Furthermore, NKR-5-3 production was induced by enterocin NKR-5-3D.

    DOI: 10.1271/bbb.110972

  • The incongruent gelatinase genotype and phenotype in Enterococcus faecalis are due to shutting off the ability to respond to the gelatinase biosynthesis-activating pheromone (GBAP) quorum-sensing signal 査読

    Neuza Teixeira, Sofia Santos, Paulo Marujo, Ryoji Yokohata, Vijayalakshmi S. Iyer, Jiro Nakayama, Lynn E. Hancock, Pascale Serror, Maria de Fátima Silva Lopes Lopes

    Microbiology   158 ( 2 )   519 - 528   2012年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    The concomitant presence of a complete fsr quorum-sensing system and gelE-sprE operons in Enterococcus faecalis is known to be essential for the detection of gelatinase activity. However, there are reports of the absence of gelatinase activity despite the presence of complete fsr and gelE loci. In order to understand this incongruence between genotype and phenotype we sequenced fsr and gelE loci of the E. faecalis LN68 strain, which was previously found to carry both operons but to lack gelatinase activity. Of the 59 nucleotide differences detected compared with the gelatinase-positive V583 strain, we found a nonsense mutation (a premature STOP codon) predicted to truncate the ATPase sensor domain of the FsrC protein, responsible for sensing and transducing the signal from the quorum-sensing molecule. Strain LN68 was highly affected in the expression of the gelE and sprE genes, further supporting the lack of Fsrdependent gelE induction. When we constructed a V583 mutant with the same premature stop mutation in the fsrC gene the resulting strain was no longer able to degrade gelatin. We conclude that the reduced ability to transduce the quorum-sensing signal of the prematurely truncated FsrC protein is sufficient to explain the negative gelatinase phenotype. As the incongruent genotype and phenotype is detected in natural isolates, we believe that the silencing of the quorum-sensing system Fsr may be beneficial for some E. faecalis strains.

    DOI: 10.1099/mic.0.055574-0

  • The incongruent gelatinase genotype and phenotype in Enterococcus faecalis are due to shutting off the ability to respond to the gelatinase biosynthesis-activating pheromone (GBAP) quorum-sensing signal. 査読 国際誌

    Neuza Teixeira, Sofia Santos, Paulo Marujo, Ryoji Yokohata, Vijayalakshmi S Iyer, Jiro Nakayama, Lynn E Hancock, Pascale Serror, Maria de Fátima Silva Lopes

    Microbiology (Reading, England)   158 ( 2 )   519 - 528   2012年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    The concomitant presence of a complete fsr quorum-sensing system and gelE-sprE operons in Enterococcus faecalis is known to be essential for the detection of gelatinase activity. However, there are reports of the absence of gelatinase activity despite the presence of complete fsr and gelE loci. In order to understand this incongruence between genotype and phenotype we sequenced fsr and gelE loci of the E. faecalis LN68 strain, which was previously found to carry both operons but to lack gelatinase activity. Of the 59 nucleotide differences detected compared with the gelatinase-positive V583 strain, we found a nonsense mutation (a premature STOP codon) predicted to truncate the ATPase sensor domain of the FsrC protein, responsible for sensing and transducing the signal from the quorum-sensing molecule. Strain LN68 was highly affected in the expression of the gelE and sprE genes, further supporting the lack of Fsr-dependent gelE induction. When we constructed a V583 mutant with the same premature stop mutation in the fsrC gene the resulting strain was no longer able to degrade gelatin. We conclude that the reduced ability to transduce the quorum-sensing signal of the prematurely truncated FsrC protein is sufficient to explain the negative gelatinase phenotype. As the incongruent genotype and phenotype is detected in natural isolates, we believe that the silencing of the quorum-sensing system Fsr may be beneficial for some E. faecalis strains.

    DOI: 10.1099/mic.0.055574-0

  • Ring A of nukacin ISK-1: a lipid II-binding motif for type-A(II) lantibiotic. 査読 国際誌

    Mohammad R Islam, Mami Nishie, Jun-ichi Nagao, Takeshi Zendo, Sandro Keller, Jiro Nakayama, Daisuke Kohda, Hans-Georg Sahl, Kenji Sonomoto

    Journal of the American Chemical Society   134 ( 8 )   3687 - 3690   2012年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Ring A of nukacin ISK-1, which is also present in different type-A(II) lantibiotics, resembles a lipid II-binding motif (TxS/TxD/EC, x denotes undefined residues) similar to that present in mersacidin (type-B lantibiotics), which suggests that nukacin ISK-1 binds to lipid II as a docking molecule. Results from our experiments on peptidoglycan precursor (UDP-MurNAc-pp) accumulation and peptide antagonism assays clearly indicated that nukacin ISK-1 inhibits cell-wall biosynthesis, accumulating lipid II precursor inside the cell, and the peptide activity can be repressed by lipid I and lipid II. Interaction analysis of nukacin ISK-1 and different ring A variants with lipid II revealed that nukacin ISK-1 and nukacin D13E (a more active variant) have a high affinity (K(D) = 0.17 and 0.19 μM, respectively) for lipid II, whereas nukacin D13A (a less active variant) showed a lower affinity, and nukacin C14S (a negative variant lacking the ring A structure) exhibited no interaction. Therefore, on the basis of the structural similarity and positional significance of the amino acids in this region, we concluded that nukacin ISK-1 binds lipid II via its ring A region and may lead to the inhibition of cell-wall biosynthesis.

    DOI: 10.1021/ja300007h

  • Isolation and characterization of enterocin W, a novel two-peptide lantibiotic produced by Enterococcus faecalis NKR-4-1. 査読 国際誌

    Naruhiko Sawa, Pongtep Wilaipun, Seisuke Kinoshita, Takeshi Zendo, Vichien Leelawatcharamas, Jiro Nakayama, Kenji Sonomoto

    Applied and environmental microbiology   78 ( 3 )   900 - 903   2012年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Enterococcus faecalis NKR-4-1 isolated from pla-ra produces a novel two-peptide lantibiotic, termed enterocin W, comprising Wα and Wβ. The structure of enterocin W exhibited similarity with that of plantaricin W. The two peptides acted synergistically, and their order of binding to the cell membrane was important for their inhibitory activity.

    DOI: 10.1128/AEM.06497-11

  • Characterization and identification of weissellicin Y and weissellicin M, novel bacteriocins produced by Weissella hellenica QU 13 査読

    Y. Masuda, T. Zendo, N. Sawa, R. H. Perez, J. Nakayama, K. Sonomoto

    Journal of Applied Microbiology   112 ( 1 )   99 - 108   2012年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Aims: To identify and characterize novel bacteriocins from Weissella hellenica QU 13. Methods and Results: Weissella hellenica QU 13, isolated from a barrel used to make Japanese pickles, produced two novel bacteriocins termed weissellicin Y and weissellicin M. The primary structures of weissellicins Y and M were determined, and their molecular masses were determined to be 4925·12 and 4968·40Da, respectively. Analysis of the DNA sequence encoding the bacteriocins revealed that they were synthesized and secreted without N-terminal extensions such as leader sequences or sec signal peptides. Weissellicin M showed significantly high and characteristic homology with enterocins L50A and L50B, produced by Enterococcus faecium L50, while weissellicin Y showed no homology with any other known bacteriocins. Both bacteriocins showed broad antimicrobial spectra, with especially high antimicrobial activity against species, which contaminate pickles, such as Bacillus coagulans, and weissellicin M showed relatively higher activity than weissellicin Y. Furthermore, the stability of weissellicin M against pH and heat was distinctively higher than that of weissellicin Y. Conclusions: Weissella hellenica QU 13 produced two novel leaderless bacteriocins, weissellicin Y and weissellicin M, and weissellicin M exhibited remarkable potency that could be employed by pickle-producing industry. Significance and Impact of the Study:  This study is the first report, which represents a complete identification and characterization of novel leaderless bacteriocins from Weissella genus. © 2011 The Authors. Journal of Applied Microbiology © 2011 The Society for Applied Microbiology.

    DOI: 10.1111/j.1365-2672.2011.05180.x

  • Enhanced production of nukacin D13E in Lactococcus lactis NZ9000 by the additional expression of immunity genes. 査読 国際誌

    Tijo Varghese Puramattathu, Mohammad R Islam, Mami Nishie, Sae Yanagihara, Jun-ichi Nagao, Ken-ichi Okuda, Takeshi Zendo, Jiro Nakayama, Kenji Sonomoto

    Applied microbiology and biotechnology   93 ( 2 )   671 - 678   2012年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Nukacin D13E (D13E) is a variant of type-A(II) lantibiotic nukacin ISK-1 produced by Staphylococcus warneri ISK-1. D13E exhibited a twofold higher specific antimicrobial activity than nukacin ISK-1 against a number of Gram-positive bacteria. We previously reported the heterologous production of D13E in Lactococcus lactis NZ9000 under the control of nisin-controlled gene expression system. In this study, we demonstrated enhanced production of D13E by the additional expression of immunity genes, nukFEG. The nukacin ISK-1 immunity, conferred by the ABC transporter complex, NukFEG, and the lantibiotic-binding protein, NukH, was not overwhelmed by D13E. The additional NukFEG resulted in a fourfold increase in the immunity level of the strain and a 5.2-fold increase in D13E production. The additional NukFEGH-expressing strain with the highest D13E immunity showed reduced level of production. Further improvement in D13E production was achieved by using pH-controlled batch fermentation.

    DOI: 10.1007/s00253-011-3563-1

  • Engineering unusual amino acids into peptides using lantibiotic synthetase

    Jun Ichi Nagao, Kouki Shioya, Yoshitaka Harada, Ken Ichi Okuda, Takeshi Zendo, Jiro Nakayama, Kenji Sonomoto

    Heterologous Gene Expression in E.coli Methods and Protocols   705   225 - 236   2011年12月

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    記述言語:英語  

    Alteration of protein structure and function by introducing unusual amino acids has great potential to develop new biological tool and to produce novel therapeutic agents. Lantibiotics produced by Gram-positive bacteria are ribosomally synthesized and post-translationally modified antimicrobial peptides. The modification enzyme involved in lantibiotic biosynthesis can catalyze the formation of unusual amino acids in the nascent lantibiotic prepeptide. Here, a novel methodology on the lantibiotic nukacin ISK-1 is described for engineering unusual amino acid residues into hexa-histidine-tagged (His-tagged) prepeptide NukA by the modification enzyme NukM in Escherichia coli. Co-expression of His-tagged NukA and NukM, purification of the resulting His-tagged prepeptide by affinity chromatography, and subsequent mass spectrometry analysis show that the prepeptide is converted into a postulated peptide with decrease in mass which results from the formation of unusual amino acids such as dehydrated amino acid, lanthionine, or 3-methyl lanthionine at the expected positions. The modified prepeptide can be readily obtained by one-step purification. This strategy will thus be a simple and powerful tool for introducing unusual amino acid residues aimed at peptide engineering.

    DOI: 10.1007/978-1-61737-967-3_13

  • The effect of including Lactobacillus reuteri KUB-AC5 during post-hatch feeding on the growth and ileum microbiota of broiler chickens 査読

    M. Nakphaichit, S. Thanomwongwattana, C. Phraephaisarn, N. Sakamoto, S. Keawsompong, J. Nakayama, S. Nitisinprasert

    Poultry Science   90 ( 12 )   2753 - 2765   2011年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    The probiotic strain Lactobacillus reuteri KUB-AC5, which was originally isolated from chicken intestine, was fed to newborn broiler chicks for the first week post-hatch. The growth and ileum microbiota of the chickens were carefully monitored for 6 wk. The inclusion of 5 log cfu/g of feed statistically increased the BW gain in the first week compared with that of the control group, but this effect did not continue thereafter. Significant effects on host feed consumption and the feed-to-growth conversion ratio were not detected. The total amount and composition of ileum bacteria were investigated by quantitative PCR and pyrosequencing of the 16S rRNA gene (rDNA), respectively, and were compared between the control and the probiotic-treated groups. The amount of total bacterial 16S rDNA in ileum samples at d 42 was 5 times higher in the probiotic group than in the control, whereas no significant difference was observed at d 21. A composition analysis revealed the establishment of lactobacillienriched microbiota in the probiotic-treated chickens at d 42. At this point, the population level and species diversity of lactobacilli were significantly enhanced compared with those of the control group. In addition, Actinobacteria, mainly genera Corynebacterium and Dietzia, were also statistically higher in the probiotic group. However, Proteobacteria, including those of the family Campylobacterales and some other nonbeneficial bacterial groups, were decreased in the probiotic group at the growing stage. Therefore, with probiotic supplementation, it was demonstrated that Lactobacillus reuteri KUB-AC5 in the early post-hatching period had a delayed effect on ileum microbiota, which resulted in the enrichment of potentially beneficial lactobacilli and the suppression of Proteobacteria, including nonbeneficial bacterial groups. © 2011 Poultry Science Association Inc.

    DOI: 10.3382/ps.2011-01637

  • Survey of Overactive Bladder Symptoms Influencing Bother Before and After Treatment With Tamsulosin Hydrochloride in Japanese Patients With Benign Prostatic Hyperplasia 査読

    Akira Tsujimura, Tetsuya Takao, Yasushi Miyagawa, Hidenobu Okuda, Keisuke Yamamoto, Shinichiro Fukuhara, Jiro Nakayama, Tomohiro Ueda, Hiroshi Kiuchi, Toshiaki Hirai, Yuichi Tsujimoto, Hidenobu Miura, Nobuteru Kanno, Makoto Higashino, Yoshihiro Nakamura, Kenji Nishimura, Norio Nonomura

    UROLOGY   78 ( 5 )   1058 - 1062   2011年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    OBJECTIVE To evaluate the relation between bother and overactive bladder (OAB) symptoms in patients with benign prostatic hyperplasia (BPH) patients using questionnaires: the BPH Impact Index (BII) and the OAB symptom score (OABSS). Annoyance from BPH usually provides the basis for a patient's decision to seek medical treatment. However, a study investigating the bother caused by OAB symptoms in patients with BPH and OAB has not been fully conducted.
    METHODS The present study included 100 male patients who were diagnosed with BPH and OAB according to questionnaire criteria. All patients were instructed to take tamsulosin for 28 days. The relation between the BII and OABSS was assessed to determine the factors influencing OAB symptoms on the BII before and after treatment.
    RESULTS The BII correlated positively with the OABSS, and multivariate analysis showed that the subscore of urgency was the only independent factor influencing the BII. Even after treatment, lower urinary tract symptoms were diagnosed as OAB using the OABSS criteria in 45 (45.0%) of the 100 patients. In these patients, the BII still correlated positively with the OABSS. However, multivariate analysis showed that the subscore of urgency incontinence, not urgency, was the only independent factor influencing the BII, although the subscore of urgency incontinence was significantly decreased with tamsulosin treatment.
    CONCLUSION The degree of bother correlated with the degree of OAB symptoms in patients with BPH and OAB at baseline and after treatment with tamsulosin. The OAB symptom causing the bother was altered by treatment with tamsulosin in these patients. UROLOGY 78: 1058-1062, 2011. (C) 2011 Elsevier Inc.

    DOI: 10.1016/j.urology.2011.05.032

  • Anti-HIV siamycin I directly inhibits autophosphorylation activity of the bacterial FsrC quorum sensor and other ATP-dependent enzyme activities. 査読 国際誌

    Pikyee Ma, Kenzo Nishiguchi, Hayley M Yuille, Lianne M Davis, Jiro Nakayama, Mary K Phillips-Jones

    FEBS letters   585 ( 17 )   2660 - 2664   2011年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Siamycin I disrupts growth and quorum sensing in Enterococcus faecalis. Using purified intact protein, we demonstrate here that quorum membrane sensor kinase FsrC is a direct target of siamycin I, reducing pheromone-stimulated autophosphorylation activity by up to 91%. Inhibition was non-competitive with ATP as substrate. Other ATP-binding enzymes were also inhibited, including nine other membrane sensor kinases of E. faecalis, Rhodobacter sphaeroides PrrB, porcine Na(+)-dependent ATPase and the catalytic subunit of bovine protein kinase A, but not bacterial β-galactosidase, confirming targeted inhibition of a wide range of ATP dependent reactions, and elucidating a likely mechanism underlying the lethality of the inhibitor.

    DOI: 10.1016/j.febslet.2011.07.026

  • Crystallization and preliminary X-ray analysis of a putative sensor histidine kinase domain: the C-terminal domain of HksP4 from Aquifex aeolicus VF5. 査読 国際誌

    Shoichiro Horita, Yosuke Yamanaka, Akihiro Yamamura, Akitoshi Okada, Jiro Nakayama, Koji Nagata, Masaru Tanokura

    Acta crystallographica. Section F, Structural biology and crystallization communications   67 ( 7 )   803 - 807   2011年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    The histidine kinase domain of the cytoplasmic protein HksP4 from the hyperthermophilic bacterium Aquifex aeolicus VF5, located in the C-terminal half of the protein, was expressed, purified and crystallized. Diffraction-quality crystals were obtained in the presence of adenosine triphosphate (ATP) or adenosine 5'-(β,γ-imido)triphosphate (AMPPNP) by the sitting-drop vapour-diffusion method using PEG 3350 as the precipitant. The crystals obtained in the presence of ATP and AMPPNP diffracted X-rays to 3.1 and 2.9 Å resolution, respectively, on BL-5A at Photon Factory (Ibaraki, Japan) and were found to belong to the same space group P2(1)2(1)2(1), with unit-cell parameters a=80.2, b=105.5, c=122.0 Å and a=81.5, b=105.5, c=130.9 Å, respectively. Their Matthews coefficients (VM=2.74 and 2.51 Å3 Da(-1), respectively) indicated that both crystals contained four protein molecules per asymmetric unit.

    DOI: 10.1107/S1744309111018434

  • Lacticin Q-mediated selective toxicity depending on physicochemical features of membrane components 査読

    Fuminori Yoneyama, Kanako Ohno, Yuichi Imura, Mengqi Li, Takeshi Zendo, Jiro Nakayama, Katsumi Matsuzaki, Kenji Sonomoto

    Antimicrobial Agents and Chemotherapy   55 ( 5 )   2446 - 2450   2011年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Lacticin Q, a lactococcal pore-forming bacteriocin, shows activity toward Gram-positive bacteria but not Gram-negative bacteria. Lacticin Q did not induce permeability of the outer membrane of Gram-negative bacteria. Experiments using model membranes containing outer membrane components suggested that lacticin Q binds to the outer membrane of Gram-negative bacteria but is unable to penetrate it. The lack of activity of lacticin Q was attributed to physicochemical features of the outer membrane components.

    DOI: 10.1128/AAC.00808-10

  • Lantibiotic transporter requires cooperative functioning of the peptidase domain and the ATP binding domain 査読

    Mami Nishie, Makoto Sasaki, Jun Ichi Nagao, Takeshi Zendo, Jiro Nakayama, Kenji Sonomoto

    Journal of Biological Chemistry   286 ( 13 )   11163 - 11169   2011年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Lantibiotics are ribosomally synthesized and post-translationally modified peptide antibiotics that contain unusual amino acids such as dehydro and lanthionine residues. Nukacin ISK-1 is a class II lantibiotic, whose precursor peptide (NukA) is modified by NukM to form modified NukA. ATP-binding cassette (ABC) transporter NukT is predicted to cleave off the N-terminal leader peptide of modified NukA and secrete the mature peptide. Multiple sequence alignments revealed that NukT has an N-terminal peptidase domain (PEP) and a C-terminal ATP binding domain (ABD). Previously, in vitro reconstitution of NukT has revealed that NukT peptidase activity depends on ATP hydrolysis. Here, we constructed a series of NukT mutants and investigated their transport activity in vivo and peptidase activity in vitro. Most of the mutations of the conserved residues of PEP or ABD resulted in failure of nukacin ISK-1 production and accumulation of modified NukA inside the cells. NukT(N106D) was found to be the only mutant capable of producing nukacin ISK-1. Asn106 is conserved as Asp in other related ABC transporters. Additionally, an in vitro peptidase assay of NukT mutants demonstrated that PEP is on the cytosolic side and all of the ABD mutants as well as PEP (with the exception of NukT(N106D)) did not have peptidase activity in vitro. Taken together, these observations suggest that the leader peptide is cleaved off inside the cells before peptide secretion; both PEP and ABD are important for NukT peptidase activity, and cooperation between these two domains inside the cells is indispensable for proper functioning of NukT.

    DOI: 10.1074/jbc.M110.212704

  • Urgency Is an Independent Factor for Sleep Disturbance in Men with Obstructive Sleep Apnea 査読

    Akira Tsujimura, Tetsuya Takao, Yasushi Miyagawa, Keisuke Yamamoto, Shinichiro Fukuhara, Jiro Nakayama, Hiroshi Kiuchi, Nakamori Suganuma, Tadashi Nakamura, Takayuki Kumano-go, Yoshiro Sugita, Norio Nonomura, Akihiko Okuyama

    UROLOGY   76 ( 4 )   967 - 970   2010年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    OBJECTIVES The relationship between overactive bladder (OAB) symptoms, other than nocturia, and sleep, has not been fully evaluated, although a close relationship between nocturia and sleep disturbance has been reported. In the present study, we evaluated the relationship between OAB symptoms and several polysomnography (PSG) parameters in middle-age men with sleep disturbance, especially to clarify whether urgency as the hallmark symptom of an OAB is independently associated with sleep quality.
    METHODS A total of 32 men >40 years of age (mean age 58.0 +/- 12.6), who had been diagnosed with obstructive sleep apnea syndrome by PSG, were included in the present study. Their OAB symptoms were evaluated using the OAB symptom score (OABSS) before PSG. The relationship between the OABSS and several parameters, such as sleeping time, sleeping efficiency, sleep latency, percentage of rapid eye movement during sleeping time, and apnea/hypopnea index obtained from PSG, was evaluated.
    RESULTS Multivariate analysis showed that only sleeping efficiency was an influencing factor on the total OABSS. Of the 4 subscores of OABSS, including frequency, nocturia, urgency, and urgency incontinence, multivariate analysis showed that the subscores of nocturia and urgency were independent influencing factors on sleeping efficiency. Nocturia correlated negatively with sleeping efficiency (Pearson's correlation 0.533, P <.01), and urgency also correlated negatively with sleeping efficiency (Pearson's correlation 0.492, P <.01).
    CONCLUSIONS We found that urgency and nocturia were factors that independently affected sleep or were affected by sleep quality, although only the association of nocturia with sleep disturbance has been the focus of previous studies. UROLOGY 76: 967-970, 2010. (C) 2010 Elsevier Inc.

    DOI: 10.1016/j.urology.2010.01.070

  • Enterocin X, a novel two-peptide bacteriocin from Enterococcus faecium KU-B5, has an antibacterial spectrum entirely different from those of its component peptides. 査読 国際誌

    Chih-Bo Hu, Wanna Malaphan, Takeshi Zendo, Jiro Nakayama, Kenji Sonomoto

    Applied and environmental microbiology   76 ( 13 )   4542 - 4545   2010年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Enterocin X, composed of two antibacterial peptides (Xalpha and Xbeta), is a novel class IIb bacteriocin from Enterococcus faecium KU-B5. When combined, Xalpha and Xbeta display variably enhanced or reduced antibacterial activity toward a panel of indicators compared to each peptide individually. In E. faecium strains that produce enterocins A and B, such as KU-B5, only one additional bacteriocin had previously been known.

    DOI: 10.1128/AEM.02264-09

  • Identification and characterization of novel multiple bacteriocins produced by Leuconostoc pseudomesenteroides QU 15 査読

    N. Sawa, K. Okamura, T. Zendo, K. Himeno, J. Nakayama, K. Sonomoto

    Journal of Applied Microbiology   109 ( 1 )   282 - 291   2010年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Aim: To characterize novel multiple bacteriocins produced by Leuconostoc pseudomesenteroides QU 15. Methods and Results: Leuconostoc pseudomesenteroides QU 15 isolated from Nukadoko (rice bran bed) produced novel bacteriocins. By using three purification steps, four antimicrobial peptides termed leucocin A (δC7), leucocin A-QU 15, leucocin Q and leucocin N were purified from the culture supernatant. The amino acid sequences of leucocin A (δC7) and leucocin A-QU 15 were identical to that of leucocin A-UAL 187 belonging to class IIa bacteriocins, but leucocin A (δC7) was deficient in seven C-terminal residues. Leucocin Q and leucocin N are novel class IId bacteriocins. Moreover, the DNA sequences encoding three bacteriocins, leucocin A-QU 15, leucocin Q and leucocin N were obtained. Conclusions: These bacteriocins including two novel bacteriocins were identified from Leuc. pseudomesenteroides QU 15. They showed similar antimicrobial spectra, but their intensities differed. The C-terminal region of leucocin A-QU 15 was important for its antimicrobial activity. Leucocins Q and N were encoded by adjacent open reading frames (ORFs) in the same operon, but leucocin A-QU 15 was not. Significance and Impact of Study: These leucocins were produced concomitantly by the same strain. Although the two novel bacteriocins were encoded by adjacent ORFs, a characteristic of class IIb bacteriocins, they did not show synergistic activity. © 2010 The Authors.

    DOI: 10.1111/j.1365-2672.2009.04653.x

  • Functional significance of the E loop, a novel motif conserved in the lantibiotic immunity ATP-binding cassette transport systems. 査読 国際誌

    Ken-ichi Okuda, Sae Yanagihara, Tomomichi Sugayama, Takeshi Zendo, Jiro Nakayama, Kenji Sonomoto

    Journal of bacteriology   192 ( 11 )   2801 - 2808   2010年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Lantibiotics are peptide-derived antibacterial substances produced by some Gram-positive bacteria and characterized by the presence of unusual amino acids, like lanthionines and dehydrated amino acids. Because lantibiotic producers may be attacked by self-produced lantibiotics, they express immunity proteins on the cytoplasmic membrane. An ATP-binding cassette (ABC) transport system mediated by the LanFEG protein complex is a major system in lantibiotic immunity. Multiple-sequence alignment analysis revealed that LanF proteins contain the E loop, a variant of the Q loop, which is a well-conserved motif in the nucleotide-binding domains (NBDs) of general ABC transporters. To elucidate E loop function, we introduced a mutation in the NukF protein, which is involved in the nukacin-ISK-1 immunity system. Amino acid replacement of glutamic acid in the E loop with glutamine (E85Q) resulted in slight decreases in the immunity level and transport activity. Additionally, the E85A mutation severely impaired the immunity level and transport activity. On the other hand, ATPase activities of purified E85Q and E85A mutants were almost similar to that of the wild type. These results suggested that the E loop found in ABC transporters involved in lantibiotic immunity plays a significant role in the function of these transporters, especially in the structural change of transmembrane domains.

    DOI: 10.1128/JB.00003-10

  • Characterization of modification enzyme NukM and engineering of a novel thioether bridge in lantibiotic nukacin ISK-1. 査読 国際誌

    Kouki Shioya, Yoshitaka Harada, Jun-ichi Nagao, Jiro Nakayama, Kenji Sonomoto

    Applied microbiology and biotechnology   86 ( 3 )   891 - 899   2010年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    The lantibiotic nukacin ISK-1 is an antimicrobial peptide containing unusual amino acids such as lanthionine and dehydrobutyrine. The nukacin ISK-1 prepeptide (NukA) undergoes posttranslational modifications, such as the dehydration and cyclization reactions required to form the unusual amino acids by the modification enzyme NukM. We have previously constructed a system for the introduction of unusual amino acids into NukA by coexpression of NukM in Escherichia coli. Using this system, we describe the substrate specificity of NukM by the coexpression of a series of NukA mutants. Our results revealed the following characteristics of NukM: (1) its dehydration activity is not coupled to its cyclization activity; (2) its dehydration activity is site-specific; (3) the length of the substrate is important for its dehydration activity. Furthermore, we succeeded in introducing a novel thioether bridge in NukA by replacing an unmodified Ser at position 27 with a Cys residue.

    DOI: 10.1007/s00253-009-2334-8

  • Cell-to-cell communications among microorganisms

    Sueharu Horinouchi, Kenji Ueda, Jiro Nakayama, Tsukasa Ikeda

    Comprehensive Natural Products II: Chemistry and Biology   4   283 - 337   2010年3月

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    記述言語:その他  

  • Effect of a negatively charged lipid on membrane-lacticin Q interaction and resulting pore formation 査読

    Fuminori Yoneyama, Kouki Shioya, Takeshi Zendo, Jiro Nakayama, Kenji Sonomoto

    Bioscience, Biotechnology and Biochemistry   74 ( 1 )   218 - 221   2010年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Lacticin Q is an antimicrobial peptide that forms pores on membranes. We investigated effects of negatively charged lipids on the binding and pore formation of lacticin Q with liposomes by surface plasmon resonance analysis and fluorescence dye leakage experiments respectively. Negatively charged lipids accelerated the binding of lacticin Q on the membranes and the resulting pore formation. However, the acceleration was not an essential factor in the killing activity of lacticin Q, since pore-forming activities against electrically neutral and negatively charged liposomes occurred similarly.

    DOI: 10.1271/bbb.90666

  • Adiponectin and testosterone in patients with symptoms of late-onset hypogonadism: Is there a link? 査読

    Akira Tsujimura, Shingo Takada, Yasuhiro Matsuoka, Jiro Nakayama, Tetsuya Takao, Yasushi Miyagawa, Mina Sonoda, Hitoshi Nishizawa, Hiromi Iwahashi, Tohru Funahashi, Norio Nonomura, Akihiko Okuyama

    INTERNATIONAL JOURNAL OF UROLOGY   16 ( 10 )   830 - 835   2009年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Objectives:
    To examine the correlation between testosterone and adiponectin in symptomatic late-onset hypogonadism (LOH) patients.
    Methods:
    The study included 174 patients (> 40 years old) with at least one LOH symptom and an Aging Male Symptoms score > 26. The correlation between serum adiponectin levels and various factors was investigated by simple and multiple regression analyses. Serum adiponectin levels before and after testosterone replacement therapy (TRT) in 43 patients with serum free testosterone < 11.8 pg/mL were also compared.
    Results:
    Serum adiponectin levels increased with increased age (P < 0.01), decreased with increased body mass index (P < 0.01), and increased with increased sex hormone-binding globulin (P < 0.01). Multiple regression analysis revealed that body mass index and sex hormone-binding globulin were factors with an influence on serum adiponectin levels. However, no association between testosterone and adiponectin was found. In the 43 patients receiving TRT, serum adiponectin levels before and after TRT did not differ significantly.
    Conclusions:
    Serum adiponectin is not related to serum testosterone in symptomatic LOH patients, suggesting that TRT in these subjects does not pose a higher risk of inducing a metabolic syndrome.

    DOI: 10.1111/j.1442-2042.2009.02376.x

  • Mapping and identification of the region and secondary structure required for the maturation of the nukacin ISK-1 prepeptide. 査読 国際誌

    Jun-Ichi Nagao, Yoshiko Morinaga, Mohammad R Islam, Sikder M Asaduzzaman, Yuji Aso, Jiro Nakayama, Kenji Sonomoto

    Peptides   30 ( 8 )   1412 - 1420   2009年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    The prepeptide NukA of the lantibiotic nukacin ISK-1 consists of an N-terminal leader peptide followed by a propeptide moiety that undergoes post-translational modifications, that is, formation of unusual amino acids by NukM, cleavage of the leader peptide and transport by NukT to yield a mature peptide. To identify the region and conformation required for the maturation of prepeptide, we expressed a series of NukA mutants, mutants with the N-terminus-truncated leader peptide and site-directed mutants with conserved residues in the leader peptide of type A(II) lantibiotics, which were evaluated on the basis of the production of nukacin ISK-1. In addition, the secondary structure data of NukA mutants or fragments were obtained by circular dichroism spectra. The results indicated the importance of the alpha-helical leader peptide with hydrophobic and hydrophilic orientation consisting of the conserved residues in type A(II) lantibiotics. The expression data from various combinations of the chimeric prepeptides consisting of NukA and LctA (the prepeptide of lacticin 481, which shows high identity with NukA) further revealed that the amino acid difference at the C-terminus of the propeptide moiety between NukA and LctA, especially His at position 15 and Phe at position 19, was important for the maturation processes by the nukacin ISK-1 biosynthetic enzymes. Our findings suggest that the determinants in NukA were critically involved in the biosynthesis of nukacin ISK-1 and would thus be important for recognition by the nukacin ISK-1 biosynthetic enzymes.

    DOI: 10.1016/j.peptides.2009.05.021

  • Peptide-lipid huge toroidal pore, a new antimicrobial mechanism mediated by a lactococcal bacteriocin, lacticin Q. 査読 国際誌

    Fuminori Yoneyama, Yuichi Imura, Kanako Ohno, Takeshi Zendo, Jiro Nakayama, Katsumi Matsuzaki, Kenji Sonomoto

    Antimicrobial agents and chemotherapy   53 ( 8 )   3211 - 3217   2009年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Lacticin Q is a pore-forming bacteriocin produced by Lactococcus lactis QU 5, and its antimicrobial activity is in the nanomolar range. Lacticin Q induced calcein leakage from negatively charged liposomes. However, no morphological changes in the liposomes were observed by light scattering. Concomitantly with the calcein leakage, lacticin Q was found to translocate from the outer to the inner leaflet of the liposomes, after it initially bound to the membrane within 2 s. Lacticin Q also induced lipid flip-flop. These results reveal that the antimicrobial mechanism of lacticin Q can be described by the toroidal pore model. This is the first report of a bacteriocin of gram-positive bacteria that forms a toroidal pore. From liposomes, lacticin Q leaked fluorescence-labeled dextran with a diameter of 4.6 nm. In addition, lacticin Q caused the leakage of small proteins, such as the green fluorescent protein, from live bacterial cells. There are no other reports of antimicrobial peptides that exhibit protein leakage properties. The proposed pore formation model of lacticin Q is as follows: (i) quick binding to outer membrane leaflets; (ii) the formation of at least 4.6-nm pores, causing protein leakage with lipid flip-flop; and (iii) the migration of lacticin Q molecules from the outer to the inner membrane leaflets. Consequently, we termed the novel pore model in the antimicrobial mechanism of lacticin Q a "huge toroidal pore."

    DOI: 10.1128/AAC.00209-09

  • Nukacin ISK-1, a bacteriostatic lantibiotic. 査読 国際誌

    Sikder M Asaduzzaman, Jun-ichi Nagao, Hiroshi Iida, Takeshi Zendo, Jiro Nakayama, Kenji Sonomoto

    Antimicrobial agents and chemotherapy   53 ( 8 )   3595 - 8   2009年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    We determined the mode of action of nukacin ISK-1. It did not cause membrane potential dissipation or the efflux of ATP or K(+) ions from the cells of a sensitive bacterial strain; however, it blocked the membrane depolarization activity of nisin. Nukacin ISK-1-treated cells had single arrangements of cells without the formation of a complete septum. A remarkable reduction in cell wall width was also observed, but cytoplasmic content was unaffected. We concluded that nukacin ISK-1 is bacteriostatic.

    DOI: 10.1128/AAC.01623-08

  • Evaluation of essential and variable residues of nukacin ISK-1 by NNK scanning 査読

    Mohammad R. Islam, K. Shioya, J. Nagao, M. Nishie, H. Jikuya, T. Zendo, J. Nakayama, K. Sonomoto

    Molecular Microbiology   72 ( 6 )   1438 - 1447   2009年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Nukacin ISK-1, a type-A(II) lantibiotic, comprises 27 amino acids with a distinct linear N-terminal and a globular C-terminal region. To identify the positional importance or redundancy of individual residues responsible for nukacin ISK-1 antimicrobial activity, we replaced the native codons of the parent peptide with NNK triplet oligonucleotides in order to generate a bank of nukacin ISK-1 variants. The bioactivity of each peptide variant was evaluated by colony overlay assay, and hence we identified three Lys residues (Lys1, Lys2 and Lys3) that provided electrostatic interactions with the target membrane and were significantly variable. The ring structure of nukacin ISK-1 was found to be crucially important as replacing the ring-forming residues caused a complete loss of bioactivity. In addition to the ring-forming residues, Gly5, His12, Asp13, Met16, Asn17 and Gln20 residues were found to be essential for antimicrobial activity; Val6, Ile7, Val10, Phe19, Phe21, Val22, Phe23 and Thr24 were relatively variable; and Ser4, Pro8, His15 and Ser27 were extensively variable relative to their positions. We obtained two variants, Asp13Glu and Val22Ile, which exhibited a twofold higher specific activity compared with the wild-type and are the first reported type-A(II) lantibiotic mutant peptides with increased potency. © 2009 Blackwell Publishing Ltd.

    DOI: 10.1111/j.1365-2958.2009.06733.x

  • TRANSPLANTATION OF OLFACTORY MUCOSA FOLLOWING SPINAL CORD INJURY IMPROVES VOIDING EFFICIENCY IN RAT 査読

    Jiro Nakayama, Keisuke Yamamoto, Shinichiro Fukuhara, Toshiaki Hirai, Tomohiro Ueda, Hiroshi Kiuchi, Tetsuya Takao, Yasushi Miyagawa, Akira Tsujimura, Akihiko Okuyama

    JOURNAL OF UROLOGY   181 ( 4 )   337 - 337   2009年4月

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    記述言語:英語  

    DOI: 10.1016/S0022-5347(09)60960-5

  • Identification and characterization of lactocyclicin Q, a novel cyclic bacteriocin produced by Lactococcus sp. strain QU 12. 査読 国際誌

    Naruhiko Sawa, Takeshi Zendo, Junko Kiyofuji, Koji Fujita, Kohei Himeno, Jiro Nakayama, Kenji Sonomoto

    Applied and environmental microbiology   75 ( 6 )   1552 - 1558   2009年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Lactococcus sp. strain QU 12, which was isolated from cheese, produced a novel cyclic bacteriocin termed lactocyclicin Q. By using cation-exchange chromatography, hydrophobic interaction chromatography, and reverse-phase high-performance liquid chromatography, lactocyclicin Q was purified from culture supernatant, and its molecular mass was determined to be 6,062.8 Da by mass spectrometry. Lactocyclicin Q has been characterized by its unique antimicrobial spectrum, high level of protease resistance, and heat stability compared to other reported bacteriocins of lactic acid bacteria. The amino acid sequence of lactocyclicin Q was determined chemically, and this compound is composed of 61 amino acid residues that have a cyclic structure with linkage between the N and C termini by a peptide bond. It showed no homology to any other antimicrobial peptide, including cyclic bacteriocins. On the basis of the amino acid sequences obtained, the sequence of the gene encoding the prepeptide lactocyclicin Q was obtained. This is the first report of a cyclic bacteriocin purified from a strain belonging to the genus Lactococcus.

    DOI: 10.1128/AEM.02299-08

  • Two-peptide bacteriocin from Lactobacillus plantarum PMU 33 strain isolated from som-fak, a Thai low salt fermented fsh product

    W. Noonpakdee, P. Jumriangrit, K. Wittayakom, J. Zendo, J. Nakayama, K. Sonomoto, S. Panyim

    Asia-Pacific Journal of Molecular Biology and Biotechnology   17 ( 1 )   19 - 25   2009年1月

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    記述言語:その他   掲載種別:研究論文(学術雑誌)  

    A total of 12,520 lactic acid bacteria (LAB) isolated from fermented fsh products "som-fak" were screened for bacteriocin. One Lactobacillus plantarum PMU33 strain produced bacteriocin that inhibited a large number of Gram-positive bacteria including food borne pathogens, Listeria monocytogenes, Bacillus cereus and Staphylococcus aureus. Biochemical studies revealed that the bacteriocin was heat stable even at autoclaving temperature (121°C for 15 min) and was active over a wide pH range (2-10). The bacteriocin purifed and characterized from the culture supernatant consists of two peptides with the molecular masses of 3222 and 3099 by mass spectrometry analysis. The molecular mass of this two-peptide bacteriocin were nearly identical to that of two-peptide plantaricin W (Plw) which consists of two peptides Plwa and Plwβ. The genes encoding these two peptides amplifed by PCR with Plw gene specifc primer showed identical sequences to Plwa and Plwβ. The bacteriocins and their producing strains isolated from som-fak may fnd application as bio-preservatives in fermented food products.

  • Two-peptide bacteriocin from Lactobacillus plantarum PMU 33 strain isolated from som-fak, a Thai low salt fermented fsh product

    W. Noonpakdee, P. Jumriangrit, K. Wittayakom, J. Zendo, J. Nakayama, K. Sonomoto, S. Panyim

    Asia-Pacific Journal of Molecular Biology and Biotechnology   17 ( 1 )   19 - 25   2009年1月

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    記述言語:その他   掲載種別:研究論文(学術雑誌)  

    A total of 12,520 lactic acid bacteria (LAB) isolated from fermented fsh products "som-fak" were screened for bacteriocin. One Lactobacillus plantarum PMU33 strain produced bacteriocin that inhibited a large number of Gram-positive bacteria including food borne pathogens, Listeria monocytogenes, Bacillus cereus and Staphylococcus aureus. Biochemical studies revealed that the bacteriocin was heat stable even at autoclaving temperature (121°C for 15 min) and was active over a wide pH range (2-10). The bacteriocin purifed and characterized from the culture supernatant consists of two peptides with the molecular masses of 3222 and 3099 by mass spectrometry analysis. The molecular mass of this two-peptide bacteriocin were nearly identical to that of two-peptide plantaricin W (Plw) which consists of two peptides Plwa and Plwβ. The genes encoding these two peptides amplifed by PCR with Plw gene specifc primer showed identical sequences to Plwa and Plwβ. The bacteriocins and their producing strains isolated from som-fak may fnd application as bio-preservatives in fermented food products.

  • Lacticin Q, a lactococcal bacteriocin, causes high-level membrane permeability in the absence of specific receptors. 査読 国際誌

    Fuminori Yoneyama, Yuichi Imura, Shiro Ichimasa, Koji Fujita, Takeshi Zendo, Jiro Nakayama, Katsumi Matsuzaki, Kenji Sonomoto

    Applied and environmental microbiology   75 ( 2 )   538 - 541   2009年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    To characterize the mode of action of lacticin Q (LnqQ), its membrane-permeabilizing activity was compared with that of nisin A because of the similar antimicrobial features of these compounds. Lipid II, the receptor for nisin A, was not required for LnqQ activity. LnqQ induced high-level membrane permeability in the absence of specific receptors.

    DOI: 10.1128/AEM.01827-08

  • Biosynthetic characterization and biochemical features of the third natural nisin variant, nisin Q, produced by Lactococcus lactis 61-14 査読

    F. Yoneyama, M. Fukao, Takeshi Zendo, Jiro Nakayama, Kenji Sonomoto

    Journal of Applied Microbiology   105 ( 6 )   1982 - 1990   2008年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Aims: To characterize the genetic and biochemical features of nisin Q. Methods and Results: The nisin Q gene cluster was sequenced, and 11 putative orfs having 82% homology with the nisin A biosynthesis gene cluster were identified. Nisin Q production was confirmed from the nisQ-introduced nisin Z producer. In the reporter assay, nisin Q exhibited an induction level that was threefold lower than that of nisin A. Nisin Q demonstrated an antimicrobial spectrum similar to those of the other nisins. Under oxidizing conditions, nisin Q retained a higher level of activity than nisin A. This higher oxidative tolerance could be attributed to the presence of only one methionine residue in nisin Q, in contrast to other nisins that contain two. Conclusions: The 11 orfs of the nisin producers were identical with regard to their functions. The antimicrobial spectra of the three natural nisins were similar. Nisin Q demonstrated higher oxidative tolerance than nisin A. Significance and Impact of the Study: Genetic and biochemical features of nisin Q are similar to those of other variants. Moreover, owing to its higher oxidative tolerance, nisin Q is a potential alternative for nisin A.

    DOI: 10.1111/j.1365-2672.2008.03958.x

  • Two homologous Agr-like quorum-sensing systems cooperatively control adherence, cell morphology, and cell viability properties in Lactobacillus plantarum WCFS1. 査読 国際誌

    Toshio Fujii, Colin Ingham, Jiro Nakayama, Marke Beerthuyzen, Ryoko Kunuki, Douwe Molenaar, Mark Sturme, Elaine Vaughan, Michiel Kleerebezem, Willem de Vos

    Journal of bacteriology   190 ( 23 )   7655 - 65   2008年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    A two-component regulatory system of Lactobacillus plantarum, encoded by genes designated lamK and lamR (hpk10 and rrp10), was studied. The lamK and lamR genes encode proteins which are highly homologous to the quorum-sensing histidine kinase LamC and the response regulator LamA, respectively. Transcription analysis of the lamKR operon and the lamBDCA operon and liquid chromatography-mass spectrometry analysis of production of the LamD558 autoinducing peptide were performed for DeltalamA, DeltalamR, DeltalamA DeltalamR deletion mutants and a wild-type strain. The results suggested that lamA and lamR are cooperating genes. In addition, typical phenotypes of the DeltalamA mutant, such as reduced adherence to glass surfaces and filamentous cell morphology, were enhanced in the DeltalamA DeltalamR mutant. Microarray analysis suggested that the same cell wall polysaccharide synthesis genes, stress response-related genes, and cell wall protein-encoding genes were affected in the DeltalamA and DeltalamA DeltalamR mutants. However, the regulation ratio was more significant for the DeltalamA DeltalamR mutant, indicating the cooperative effect of LamA and LamR.

    DOI: 10.1128/JB.01489-07

  • Biosynthetic characterization and biochemical features of the third natural nisin variant, nisin Q, produced by Lactococcus lactis 61-14 査読

    F. Yoneyama, M. Fukao, T. Zendo, J. Nakayama, K. Sonomoto

    Journal of Applied Microbiology   105 ( 6 )   1982 - 1990   2008年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Aims: To characterize the genetic and biochemical features of nisin Q. Methods and Results: The nisin Q gene cluster was sequenced, and 11 putative orfs having 82% homology with the nisin A biosynthesis gene cluster were identified. Nisin Q production was confirmed from the nisQ-introduced nisin Z producer. In the reporter assay, nisin Q exhibited an induction level that was threefold lower than that of nisin A. Nisin Q demonstrated an antimicrobial spectrum similar to those of the other nisins. Under oxidizing conditions, nisin Q retained a higher level of activity than nisin A. This higher oxidative tolerance could be attributed to the presence of only one methionine residue in nisin Q, in contrast to other nisins that contain two. Conclusions: The 11 orfs of the nisin producers were identical with regard to their functions. The antimicrobial spectra of the three natural nisins were similar. Nisin Q demonstrated higher oxidative tolerance than nisin A. Significance and Impact of the Study: Genetic and biochemical features of nisin Q are similar to those of other variants. Moreover, owing to its higher oxidative tolerance, nisin Q is a potential alternative for nisin A. © 2008 The Authors.

    DOI: 10.1111/j.1365-2672.2008.03958.x

  • Binding specificity of the lantibiotic-binding immunity protein NukH. 査読 国際誌

    Ken-ichi Okuda, Sae Yanagihara, Kouki Shioya, Yoshitaka Harada, Jun-ichi Nagao, Yuji Aso, Takeshi Zendo, Jiro Nakayama, Kenji Sonomoto

    Applied and environmental microbiology   74 ( 24 )   7613 - 7619   2008年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    NukH is a lantibiotic-binding immunity protein that shows strong binding activity against type A(II) lantibiotics. In this study, the binding specificity of NukH was analyzed by using derivatives of nukacin ISK-1, which is a type A(II) lantibiotic produced by Staphylococcus warneri ISK-1. Interactions between cells of Lactococcus lactis transformants expressing nukH and nukacin ISK-1 derivatives were analyzed by using a quantitative peptide-binding assay. Differences in the cell-binding rates of each nukacin ISK-1 derivative suggested that three lysine residues at positions 1 to 3 of nukacin ISK-1 contribute to the effective binding of nukacin ISK-1 to nukH-expressing cells. The binding levels of mutants with lanthionine and dehydrobutyrine substitutions (S11A nukacin(4-27) and T24A nukacin(4-27), respectively) to nukH-expressing cells were considerably lower than those of nukacin(4-27), suggesting that unusual amino acids play a decisive role in NukH recognition. Additionally, it was suggested that T9A nukacin(4-27), a mutant with a 3-methyllanthionine substitution, binds to NukH via an intermolecular disulfide bond after it is weakly recognized by NukH. We succeeded in the detection of specific type A(II) lantibiotics from the culture supernatants of various bacteriocin producers by using the binding specificity of nukH-expressing cells.

    DOI: 10.1128/AEM.00789-08

  • Identification and partial characterization of pediocin PA-1 producing Pediococcus pentosaceus associated in traditional Thai fermented beef (mum) 査読

    Adisorn Swetwiwathana, Yaowalak Surapantapisit, Takeshi Zendo, Jiro Nakayama, Kenji Sonomoto

    JOURNAL OF BIOTECHNOLOGY   136   S748 - S749   2008年10月

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    記述言語:英語  

    DOI: 10.1016/j.jbiotec.2008.07.1781

  • Description of durancin TW-49M, a novel enterocin B-homologous bacteriocin in carrot-isolated Enterococcus durans QU 49 査読

    C. B. Hu, Takeshi Zendo, Jiro Nakayama, Kenji Sonomoto

    Journal of Applied Microbiology   105 ( 3 )   681 - 690   2008年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Aims: To characterize the novel bacteriocin produced by Enterococcus durans. Methods and Results: Enterococcus durans QU 49 was isolated from carrot and expressed bactericidal activity over 20-43°C. Bacteriocins were purified to homogeneity using the three-step purification method, one of which, termed durancin TW-49M, was an enterocin B-homologous peptide with most identical residues occurring in the N-terminus. Durancin TW-49M was more tolerant in acidic than in alkali. DNA sequencing analysis revealed durancin TW-49M was translated as a prepeptide of the double-glycine type. Durancin TW-49M and enterocin B expressed similar antimicrobial spectra, in which no significant variation due to the diversity in their C-termini was observed. Conclusions: Durancin TW-49M, a novel nonpediocin-like class II bacteriocin, was characterized to the amino acid and genetic levels. The diverse C-terminal parts of durancin TW-49M and enterocin B were hardly to be suggested as the place determining the target cell specificity. Significance and Impact of the Study: This is the first and comprehensive study of a novel bacteriocin produced by Ent. durans. The high homology at the N-terminal halves between durancin TW-49M and enterocin B makes them suitable to study the structure-function relationship of bacteriocins and their immunity proteins. © 2008 The Authors.

    DOI: 10.1111/j.1365-2672.2008.03798.x

  • Expression, purification and activities of the entire family of intact membrane sensor kinases from Enterococcus faecalis. 査読 国際誌

    Pikyee Ma, Hayley M Yuille, Victor Blessie, Nadine Göhring, Zsófia Iglói, Kenzo Nishiguchi, Jiro Nakayama, Peter J F Henderson, Mary K Phillips-Jones

    Molecular membrane biology   25 ( 6-7 )   449 - 473   2008年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Two-component signal transduction systems are the main mechanism by which bacteria sense and respond to their environment, and their membrane-located histidine protein kinases generally constitute the sensory components of these systems. Relatively little is known about their fundamental mechanisms and precise nature of the molecular signals sensed, because of the technical challenges of producing sufficient quantities of these hydrophobic membrane proteins. This study evaluated the heterologous production, purification and activities of the 16 intact membrane sensor kinases of Enterococcus faecalis. Following the cloning of the genes into expression plasmid pTTQ18His, all but one kinase was expressed successfully in Escherichia coli inner membranes. Purification of the hexa-histidine 'tagged' recombinant proteins was achieved for 13, and all but one were verified as intact. Thirteen intact kinases possessed autophosphorylation activity with no added signal when assayed in membrane vesicles or as purified proteins. Signal testing of two functionally-characterized kinases, FsrC and VicK, was successful examplifying the potential use of in vitro activity assays of intact proteins for systematic signal identification. Intact FsrC exhibited an approximately 10-fold increase in activity in response to a two-fold molar excess of synthetic GBAP pheromone, whilst glutathione, and possibly redox potential, were identified for the first time as direct modulators of VicK activity in vitro. The impact of DTT on VicK phosphorylation resulted in increased levels of phosphorylated VicR, the downstream response regulator, thereby confirming the potential of this in vitro approach for investigations of modulator effects on the entire signal transduction process of two-component systems.

    DOI: 10.1080/09687680802359885

  • Identification of the nukacin KQU-131, a new type-A(II) lantibiotic produced by Staphylococcus hominis KQU-131 isolated from Thai fermented fish product (Pla-ra). 査読 国際誌

    Pongtep Wilaipun, Takeshi Zendo, Ken-ichi Okuda, Jiro Nakayama, Kenji Sonomoto

    Bioscience, biotechnology, and biochemistry   72 ( 8 )   2232 - 2235   2008年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Staphylococcus hominis KQU-131, isolated from Thai fermented marine fish, produces a heat stable bacteriocin. Structural and genetic analysis indicated that the bacteriocin is a variant of nukacin ISK-1, a type-A(II) lantibiotic, and we termed the bacteriocin nukacin KQU-131. There were three different amino acid residues between nukacin ISK-1 and nukacin KQU-131, one residue in the leader peptide and the other two in the mature peptide.

    DOI: 10.1271/bbb.80239

  • Complete covalent structure of nisin Q, new natural nisin variant, containing post-translationally modified amino acids. 査読 国際誌

    Masanori Fukao, Takayuki Obita, Fuminori Yoneyama, Daisuke Kohda, Takeshi Zendo, Jiro Nakayama, Kenji Sonomoto

    Bioscience, biotechnology, and biochemistry   72 ( 7 )   1750 - 5   2008年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    The third member of the nisin variant, nisin Q, produced by Lactococcus lactis 61-14, is a ribosomally-synthesized antimicrobial peptide, the so-called lantibiotic containing post-translationally modified amino acids such as lanthionine and dehydroalanine. Here, we determined the complete covalent structure of nisin Q, consisting of 34 amino acids, by two-dimensional (1)H nuclear magnetic resonance (NMR) spectroscopy. Sequential assignment of nisin Q containing the unusual amino acids was performed by total correlation spectroscopy (TOCSY) and nuclear Overhauser enhancement spectroscopy (NOESY). The observed long range nuclear Overhauser effect (NOE) in nisin Q indicated assignment of all five sets of lanthionines that intramolecularly bridge residues 3-7, 8-11, 13-19, 23-26, and 25-28. Consequently, the covalent structure of nisin Q was determined to hold the same thioether linkage formation as the other two nisins, but to harbor the four amino acid substitutions, in contrast with nisin A.

    DOI: 10.1271/bbb.80066

  • In vivo and in vitro complementation study comparing the function of DnaK chaperone systems from halophilic lactic acid bacterium Tetragenococcus halophilus and Escherichia coli. 査読 国際誌

    Shinya Sugimoto, Kozue Saruwatari, Chihana Higashi, Keigo Tsuruno, Shunsuke Matsumoto, Jiro Nakayama, Kenji Sonomoto

    Bioscience, biotechnology, and biochemistry   72 ( 3 )   811 - 22   2008年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    In this study, we characterized the DnaK chaperone system from Tetragenococcus halophilus, a halophilic lactic acid bacterium. An in vivo complementation test showed that under heat stress conditions, T. halophilus DnaK did not rescue the growth of the Escherichia coli dnaK deletion mutant, whereas T. halophilus DnaJ and GrpE complemented the corresponding mutations of E. coli. Purified T. halophilus DnaK showed intrinsic weak ATPase activity and holding chaperone activity in vitro, but T. halophilus DnaK did not cooperate with the purified DnaJ and GrpE from either T. halophilus or E. coli in ATP hydrolysis or luciferase-refolding reactions under the conditions tested. E. coli DnaK, however, cross-reacted with those from both bacteria. This difference in the cooperation with DnaJ and GrpE appears to result in an inability of T. halophilus DnaK to replace the in vivo function of the DnaK chaperone of E. coli.

    DOI: 10.1271/bbb.70691

  • Bacteriocin detection by liquid chromatography/mass spectrometry for rapid identification 査読

    T. Zendo, J. Nakayama, K. Fujita, K. Sonomoto

    Journal of Applied Microbiology   104 ( 2 )   499 - 507   2008年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Aims: To establish a new system to detect and identify bacteriocins in the early stage of screening for novel bacteriocins. Methods and Results: Liquid chromatography/mass spectrometry (LC/MS) was employed for development of a new system for rapid detection and identification of bacteriocins. The system detected and identified bacteriocins such as nisin and lacticin 481 from 25 μl of culture supernatants of their producing strains by accurate mass determination coupled with simultaneous impurity removal within 40 min. Especially, the system clearly distinguished three nisin variants (A, Z, Q) in culture supernatants of their producing strains, although they have similar structures and molecular masses. Each one-step pretreatment by cell adsorption-desorption or acetone precipitation improved bacteriocin detection dramatically, especially for mundticin KS. This system could be applied for detection and molecular mass determination of novel bacteriocins by extracting bacteriocin-related ions. Conclusions: The developed system could detect and identify some kinds of bacteriocin from culture supernatants or pretreated samples. Significance and Impact of the Study: The developed system helps us to identify bacteriocins in the early stage of screening without any or with one-step pretreatment. This system is effective on not only detection of known bacteriocins but also identification of novel bacteriocins. Consequently, this system will accelerate discovery of novel bacteriocins. © 2007 The Authors.

    DOI: 10.1111/j.1365-2672.2007.03575.x

  • Complete covalent structure of nisin Q, new natural nisin variant, containing post-translationally modified amino acids 査読

    Masanori Fukao, Takayuki Obita, Fuminori Yoneyama, Daisuke Kohda, Takeshi Zendo, Jiro Nakayama, Kenji Sonomoto

    Bioscience, Biotechnology and Biochemistry   72 ( 7 )   1750 - 1755   2008年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    The third member of the nisin variant, nisin Q, produced by Lactococcus lactis 61-14, is a ribosomally-synthesized antimicrobial peptide, the so-called lantibiotic containing post-translationally modified amino acids such as lanthionine and dehydroalanine. Here, we determined the complete covalent structure of nisin Q, consisting of 34 amino acids, by two-dimensional 1H nuclear magnetic resonance (NMR) spectroscopy. Sequential assignment of nisin Q containing the unusual amino acids was performed by total correlation spectroscopy (TOCSY) and nuclear Overhauser enhancement spectroscopy (NOESY). The observed long range nuclear Overhauser effect (NOE) in nisin Q indicated assignment of all five sets of lanthionines that intramolecularly bridge residues 3-7, 8-11, 13-19, 23-26, and 25-28. Consequently, the covalent structure of nisin Q was determined to hold the same thioether linkage formation as the other two nisins, but to harbor the four amino acid substitutions, in contrast with nisin A.

    DOI: 10.1271/bbb.80066

  • Cooperative transport between NukFEG and NukH in immunity against the lantibiotic nukacin ISK-1 produced by Staphylococcus warneri ISK-1. 査読 国際誌

    Ken-ichi Okuda, Yuji Aso, Jiro Nakayama, Kenji Sonomoto

    Journal of bacteriology   190 ( 1 )   356 - 62   2008年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Nukacin ISK-1 is a lantibiotic produced by Staphylococcus warneri ISK-1. Previous studies have reported that the self-protection system of the nukacin ISK-1 producer involves the cooperative function of the ABC transporter NukFEG and the lantibiotic-binding immunity protein NukH. In this study, the cooperative mechanism between NukFEG and NukH was characterized by using fluorescein-4-isothiocyanate (FITC)-labeled nukacin ISK-1 (FITC-nuk) to clarify the localization of nukacin ISK-1 in the immunity process. Lactococcus lactis recombinants expressing nukFEGH, nukFEG, or nukH showed immunity against FITC-nuk, suggesting that FITC-nuk was recognized by the self-protection system against nukacin ISK-1. Analysis of the interaction between FITC-nuk and energy-deprived cells of the L. lactis recombinants showed that FITC-nuk specifically bound to cells expressing nukH. The interaction between FITC-nuk and nukH-expressing cells was inhibited by the addition of unlabeled nukacin ISK-1 and its derivatives with deletions of the N-terminal tail region, but not by the addition of a synthesized N-terminal tail region. This suggests that the NukH protein recognizes the C-terminal ring region of nukacin ISK-1. The addition of glucose to nukFEGH-expressing cells treated with FITC-nuk resulted in a time-dependent decrease in fluorescence intensity, indicating that FITC-nuk was transported from the cell membrane by the NukFEG protein. These results revealed that after being captured by NukH in an energy-independent manner, nukacin ISK-1 was transported to the extracellular space by NukFEG in an energy-dependent manner.

    DOI: 10.1128/JB.01300-07

  • In vivo and in vitro complementation study comparing the function of DnaK chaperone systems from halophilic lactic acid bacterium Tetragenococcus halophilus and Escherichia coli 査読

    Shinya Sugimoto, Kozue Saruwatari, Chihana Higashi, Keigo Tsuruno, Shunsuke Matsumoto, Jiro Nakayama, Kenji Sonomoto

    Bioscience, Biotechnology and Biochemistry   72 ( 3 )   811 - 822   2008年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    In this study, we characterized the DnaK chaperone system from Tetragenococcus halophilus, a halophilic lactic acid bacterium. An in vivo complementation test showed that under heat stress conditions, T. halophilus DnaK did not rescue the growth of the Escherichia coli dnaK deletion mutant, whereas T. halophilus DnaJ and GrpE complemented the corresponding mutations of E. coli. Purified T. halophilus DnaK showed intrinsic weak ATPase activity and holding chaperone activity in vitro, but T. halophilus DnaK did not cooperate with the purified DnaJ and GrpE from either T. halophilus or E. coli in ATP hydrolysis or luciferase-refolding reactions under the conditions tested. E. coli DnaK, however, cross-reacted with those from both bacteria. This difference in the cooperation with DnaJ and GrpE appears to result in an inability of T. halophilus DnaK to replace the in vivo function of the DnaK chaperone of E. coli.

    DOI: 10.1271/bbb.70691

  • Cooperative transport between NukFEG and NukH in immunity against the lantibiotic nukacin ISK-1 produced by Staphylococcus warneri ISK-1 査読

    Ken Ichi Okuda, Yuji Aso, Jiro Nakayama, Kenji Sonomoto

    Journal of Bacteriology   190 ( 1 )   356 - 362   2008年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Nukacin ISK-1 is a lantibiotic produced by Staphylococcus warneri ISK-1. Previous studies have reported that the self-protection system of the nukacin ISK-1 producer involves the cooperative function of the ABC transporter NukFEG and the lantibiotic-binding immunity protein NukH. In this study, the cooperative mechanism between NukFEG and NukH was characterized by using fluorescein-4-isothiocyanate (FITC)-labeled nukacin ISK-1 (FITC-nuk) to clarify the localization of nukacin ISK-1 in the immunity process. Lactococcus lactis recombinants expressing nukFEGH, nukFEG, or nukH showed immunity against FITC-nuk, suggesting that FITC-nuk was recognized by the self-protection system against nukacin ISK-1. Analysis of the interaction between FITC-nuk and energy-deprived cells of the L. lactis recombinants showed that FITC-nuk specifically bound to cells expressing nukH. The interaction between FITC-nuk and nukH-expressing cells was inhibited by the addition of unlabeled nukacin ISK-1 and its derivatives with deletions of the N-terminal tail region, but not by the addition of a synthesized N-terminal tail region. This suggests that the NukH protein recognizes the C-terminal ring region of nukacin ISK-1. The addition of glucose to nukFEGH-expressing cells treated with FITC-nuk resulted in a time-dependent decrease in fluorescence intensity, indicating that FITC-nuk was transported from the cell membrane by the NukFEG protein. These results revealed that after being captured by NukH in an energy-independent manner, nukacin ISK-1 was transported to the extracellular space by NukFEG in an energy-dependent manner.

    DOI: 10.1128/JB.01300-07

  • Characterization and structure analysis of a novel bacteriocin, lacticin Z, produced by Lactococcus lactis QU 14 査読

    Shun Iwatani, Takeshi Zendo, Fuminori Yoneyama, Jiro Nakayama, Kenji Sonomoto

    Bioscience, Biotechnology and Biochemistry   71 ( 8 )   1984 - 1992   2007年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    A novel bacteriocin, lacticin Z, produced by Lactococcus lactis QU 14 isolated from a horse's intestinal tract was identified. Lacticin Z was purified through a three step procedure comprised of hydrophobic-interaction, cation-exchange chromatography, and reverse-phase HPLC. ESI-TOF MS determined the molecular mass of lacticin Z to be 5,968.9 Da. The primary structure of lacticin Z was found to consist of 53 amino acid residues without any leader sequence or signal peptide. Lacticin Z showed homology to lacticin Q from L. lactis QU 5, aureocin A53 from Staphylococcus aureus A53, and mutacin BHT-B from Streptococcus rattus strain BHT. It exhibited a nanomolar range of MICs against various Gram-positive bacteria, and the activity was completely stable up to 100°C. Unlike many of other LAB bacteriocins, the stability of lacticin Z was emphasized under alkaline conditions rather than acidic conditions. All the results indicated that lacticin Z belongs to a novel type of bacteriocin.

    DOI: 10.1271/bbb.70169

  • Characterization, N-terminal sequencing and classification of cerein MRX1, a novel bacteriocin purified from a newly isolated bacterium: Bacillus cereus MRX1 査読

    S. Sebei, T. Zendo, A. Boudabous, J. Nakayama, K. Sonomoto

    Journal of Applied Microbiology   103 ( 5 )   1621 - 1631   2007年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Aim: To purify and characterize the bacteriocin produced by strain MRX1. Methods and Results: A bacteriocin-producing strain was isolated and identified as Bacillus cereus. The bacteriocin, called cerein MRX1, was purified from the culture supernatant using hydrophobic interaction, cation-exchange chromatography and RP-HPLC. It could also be purified in abundance from the cell surfaces of the producer strain. Mass spectrometry revealed its molecular mass of 3137.93 Da. Sequencing of chemically modified bacteriocin identified its partial sequence: DWTCWSCLVCAACSVELL. Amino acid analysis, confirmed by H-NMR, suggested cerein MRX1 to be a class II bacteriocin. This bacteriocin was remarkably hydrophobic, heat-stable and could withstand a wide range of pH. It exhibited a bactericidal mode of action against Bacillus coagulans JCM 2257 . Cerein MRX1 was especially active against spoilage bacteria such as Bacillus subtilis and Listeria innocua (MICs in the 1 μg ml range). In contrast, lactic acid bacteria were resistant or required higher concentrations to be inhibited. Conclusions: Cerein MRX1 is similar by its N-terminal sequence to thuricin 17 recently isolated from Bacillus thuringiensis NEB17. However, the two bacteriocins are different by their molecular masses and amino acid compositions. Significance and Impact of the Study: Chemical stability of cerein MRX1 and its ability to inhibit a large number of undesirable bacteria may give an advantage to its food or clinical application as an antibacterial agent. © 2007 The Authors. 1 T -1

    DOI: 10.1111/j.1365-2672.2007.03395.x

  • Characterization, N-terminal sequencing and classification of cerein MRX1, a novel bacteriocin purified from a newly isolated bacterium Bacillus cereus MRX1 査読

    S. Sebei, Takeshi Zendo, A. Boudabous, Jiro Nakayama, Kenji Sonomoto

    Journal of Applied Microbiology   103 ( 5 )   1621 - 1631   2007年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Aim: To purify and characterize the bacteriocin produced by strain MRX1. Methods and Results: A bacteriocin-producing strain was isolated and identified as Bacillus cereus. The bacteriocin, called cerein MRX1, was purified from the culture supernatant using hydrophobic interaction, cation-exchange chromatography and RP-HPLC. It could also be purified in abundance from the cell surfaces of the producer strain. Mass spectrometry revealed its molecular mass of 3137.93 Da. Sequencing of chemically modified bacteriocin identified its partial sequence: DWTCWSCLVCAACSVELL. Amino acid analysis, confirmed by 1H-NMR, suggested cerein MRX1 to be a class II bacteriocin. This bacteriocin was remarkably hydrophobic, heat-stable and could withstand a wide range of pH. It exhibited a bactericidal mode of action against Bacillus coagulans JCM 2257T. Cerein MRX1 was especially active against spoilage bacteria such as Bacillus subtilis and Listeria innocua (MICs in the 1 μg ml-1 range). In contrast, lactic acid bacteria were resistant or required higher concentrations to be inhibited. Conclusions: Cerein MRX1 is similar by its N-terminal sequence to thuricin 17 recently isolated from Bacillus thuringiensis NEB17. However, the two bacteriocins are different by their molecular masses and amino acid compositions. Significance and Impact of the Study: Chemical stability of cerein MRX1 and its ability to inhibit a large number of undesirable bacteria may give an advantage to its food or clinical application as an antibacterial agent.

    DOI: 10.1111/j.1365-2672.2007.03395.x

  • Differences in developing intestinal microbiota between allergic and non-allergic infants: a pilot study in Japan. 査読 国際誌

    Prapa Songjinda, Jiro Nakayama, Atsushi Tateyama, Shigemitsu Tanaka, Mina Tsubouchi, Chikako Kiyohara, Taro Shirakawa, Kenji Sonomoto

    Bioscience, biotechnology, and biochemistry   71 ( 9 )   2338 - 42   2007年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    The bacterial compositions of feces were monitored in the first 2 months for 15 infants born in Japan, including eight subjects who developed allergy by the age of 2 years. Primer sets targeting six predominant bacterial groups in the infant intestine, Bacteroidaceae, Enterobacteriaceae, bifidobacteria, enterococci, lactobacilli, and the Clostridium perfringens group, were used for real-time PCR to quantitate each population in the feces. The population of Bacteroidaceae was significantly higher in the allergic group at the ages of 1 month (P=0.03) and 2 months (P=0.05) than in the non-allergic group, while no statistically significant difference was observed for the other bacterial populations.

    DOI: 10.1271/bbb.70154

  • Characterization and structure analysis of a novel bacteriocin, lacticin Z, produced by Lactococcus lactis QU 14. 査読 国際誌

    Shun Iwatani, Takeshi Zendo, Fuminori Yoneyama, Jiro Nakayama, Kenji Sonomoto

    Bioscience, biotechnology, and biochemistry   71 ( 8 )   1984 - 92   2007年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    A novel bacteriocin, lacticin Z, produced by Lactococcus lactis QU 14 isolated from a horse's intestinal tract was identified. Lacticin Z was purified through a three step procedure comprised of hydrophobic-interaction, cation-exchange chromatography, and reverse-phase HPLC. ESI-TOF MS determined the molecular mass of lacticin Z to be 5,968.9 Da. The primary structure of lacticin Z was found to consist of 53 amino acid residues without any leader sequence or signal peptide. Lacticin Z showed homology to lacticin Q from L. lactis QU 5, aureocin A53 from Staphylococcus aureus A53, and mutacin BHT-B from Streptococcus rattus strain BHT. It exhibited a nanomolar range of MICs against various Gram-positive bacteria, and the activity was completely stable up to 100 degrees C. Unlike many of other LAB bacteriocins, the stability of lacticin Z was emphasized under alkaline conditions rather than acidic conditions. All the results indicated that lacticin Z belongs to a novel type of bacteriocin.

    DOI: 10.1271/bbb.70169

  • A gram-negative characteristic segment in Escherichia coli DnaK is essential for the ATP-dependent cooperative function with the co-chaperones DnaJ and GrpE. 査読 国際誌

    Shinya Sugimoto, Chihana Higashi, Kozue Saruwatari, Jiro Nakayama, Kenji Sonomoto

    FEBS letters   581 ( 16 )   2993 - 2999   2007年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    We describe importance of the characteristic segment in ATPase domain of DnaK chaperone which is present in all gram-negative bacteria but is absent in all gram-positive bacteria. In vitro studies, ATPase activity, luciferase-refolding activity, and surface plasmon resonance analyses, demonstrated that a segment-deletion mutant DnaKDelta74-96 became defective in the cooperation with the co-chaperones DnaJ and GrpE. In addition, in vivo complementation assay showed that expression of DnaKDelta74-96 could not rescue the viability of Escherichia coli DeltadnaK mutant at 43 degrees C. Consequently, we suggest evolutionary significance for this DnaK ATPase domain segment in gram-negative bacteria towards the DnaK chaperone system.

    DOI: 10.1016/j.febslet.2007.05.055

  • Structural analysis and characterization of lacticin Q, a novel bacteriocin belonging to a new family of unmodified bacteriocins of gram-positive bacteria. 査読 国際誌

    Koji Fujita, Shiro Ichimasa, Takeshi Zendo, Shoko Koga, Fuminori Yoneyama, Jiro Nakayama, Kenji Sonomoto

    Applied and environmental microbiology   73 ( 9 )   2871 - 3877   2007年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Lactococcus lactis QU 5 isolated from corn produces a novel bacteriocin, termed lacticin Q. By acetone precipitation, cation-exchange chromatography, and reverse-phase high-performance liquid chromatography, lacticin Q was purified from the culture supernatant of this organism, and its molecular mass was determined to be 5,926.50 Da by mass spectrometry. Subsequent analyses of amino acid and DNA sequences revealed that lacticin Q comprised 53 amino acid residues and that its N-terminal methionine residue was formylated. In contrast to most bacteriocins produced by gram-positive bacteria, lacticin Q had no N-terminal extensions such as leader or signal sequences. It showed 66% and 48% identity to AucA, a hypothetical protein from Corynebacterium jeikeium plasmid pA501, and aureocin A53, a bacteriocin from Staphylococcus aureus A53, respectively. The characteristics of lacticin Q were determined and compared to those of nisin A. Similar to nisin A, lacticin Q exhibited antibacterial activity against various gram-positive bacteria. Lacticin Q was very stable against heat treatment and changes in pH; in particular, it was stable at alkaline pH values, while nisin A was inactivated. Moreover, lacticin Q induced ATP efflux from a Listeria sp. strain in a shorter time and at a lower concentration than nisin A, indicating that the former affected indicator cells in a different manner from that of the latter. The results described here clarified the fact that lacticin Q belongs to a new family of class II bacteriocins and that it can be employed as an alternative to or in combination with nisin A.

    DOI: 10.1128/AEM.02286-06

  • Cooperative transport mechanism between NukFEG and NukH in immunity process against the lantibiotic nukacin ISK-1 produced by Staphylococcus warneri ISK-1 査読 国際誌

    Okuda, K., Y. Aso, J. Nakayama, K. Sonomoto

    J. Bacteriol.,   2007年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • Maturation of Nahm ? a Thai fermented meat product. Effect of pediocin PA-1 producer (Pediococcus pentosaceus TISTR 536) as starter culture, nitrite and garlic on Salmonella anatum during Nham fermentation 査読 国際誌

    Swetwiwathana, A., L. Napha, J. Nakayama, K. Sonomoto

    Fleischwirtschaft International   22 ( 3 )   46 - 49   2007年1月

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    記述言語:英語  

  • Structural and functional conversion of molecular chaperone ClpB from the gram-positive halophilic lactic acid bacterium Tetragenococcus halophilus mediated by ATP and stress 査読

    Shinya Sugimoto, Hiroyuki Yoshida, Yoshimitsu Mizunoe, Keigo Tsuruno, Jiro Nakayama, Kenji Sonomoto

    Journal of Bacteriology   188 ( 23 )   8070 - 8078   2006年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    In this study, we report the purification, initial structural characterization, and functional analysis of the molecular chaperone ClpB from the gram-positive, halophilic lactic acid bacterium Tetragenococcus halophilus. A recombinant T. halophilus ClpB (ClpBTha) was overexpressed in Escherichia coli and purified by affinity chromatography, hydroxyapatite chromatography, and gel filtration chromatography. As demonstrated by gel filtration chromatography, chemical cross-linking with glutaraldehyde, and electron microscopy, ClpBTha forms a homohexameric single-ring structure in the presence of ATP under nonstress conditions. However, under stress conditions, such as high-temperature (>45°C) and high-salt concentrations (>1 M KCl), it dissociated into dimers and monomers, regardless of the presence of ATP. The hexameric ClpBTha reactivated heat-aggregated proteins dependent upon the DnaK system from T. halophilus (KJETha) and ATP. Interestingly, the mixture of dimer and monomer ClpBTha, which was formed under stress conditions, protected substrate proteins from thermal inactivation and aggregation in a manner similar to those of general molecular chaperones. From these results, we hypothesize that ClpBTha forms dimers and monomers to function as a holding chaperone under stress conditions, whereas it forms a hexamer ring to function as a disaggregating chaperone in cooperation with KJETha and ATP under poststress conditions.

    DOI: 10.1128/JB.00404-06

  • Structural and functional conversion of molecular chaperone ClpB from the gram-positive halophilic lactic acid bacterium Tetragenococcus halophilus mediated by ATP and stress. 査読 国際誌

    Shinya Sugimoto, Hiroyuki Yoshida, Yoshimitsu Mizunoe, Keigo Tsuruno, Jiro Nakayama, Kenji Sonomoto

    Journal of bacteriology   188 ( 23 )   8070 - 8   2006年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    In this study, we report the purification, initial structural characterization, and functional analysis of the molecular chaperone ClpB from the gram-positive, halophilic lactic acid bacterium Tetragenococcus halophilus. A recombinant T. halophilus ClpB (ClpB(Tha)) was overexpressed in Escherichia coli and purified by affinity chromatography, hydroxyapatite chromatography, and gel filtration chromatography. As demonstrated by gel filtration chromatography, chemical cross-linking with glutaraldehyde, and electron microscopy, ClpB(Tha) forms a homohexameric single-ring structure in the presence of ATP under nonstress conditions. However, under stress conditions, such as high-temperature (>45 degrees C) and high-salt concentrations (>1 M KCl), it dissociated into dimers and monomers, regardless of the presence of ATP. The hexameric ClpB(Tha) reactivated heat-aggregated proteins dependent upon the DnaK system from T. halophilus (KJE(Tha)) and ATP. Interestingly, the mixture of dimer and monomer ClpB(Tha), which was formed under stress conditions, protected substrate proteins from thermal inactivation and aggregation in a manner similar to those of general molecular chaperones. From these results, we hypothesize that ClpB(Tha) forms dimers and monomers to function as a holding chaperone under stress conditions, whereas it forms a hexamer ring to function as a disaggregating chaperone in cooperation with KJE(Tha) and ATP under poststress conditions.

    DOI: 10.1128/JB.00404-06

  • Lantibiotics: insight and foresight for new paradigm. 査読

    Jun-ichi Nagao, Sikder M Asaduzzaman, Yuji Aso, Ken-Ichi Okuda, Jiro Nakayama, Kenji Sonomoto

    Journal of bioscience and bioengineering   102 ( 3 )   139 - 49   2006年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Lantibiotics are a unique type of antimicrobial peptide produced by a large number of gram-positive bacteria that contain unusual amino acids, such as lanthionine and dehydrated amino acids. Ribosomally synthesized lantibiotic prepeptide consists of an N-terminal leader peptide followed by a C-terminal propeptide moiety that undergoes several post-translational modification events to yield a biologically active lantibiotic. Research on lantibiotics has drawn much attention in recent years and has undergone extensive progress as a step forward to the next paradigm. Unusual amino acids in lantibiotics solely contribute to their biological activity and also enhance their structural stability. Thus, enzymes involved in lantibiotic biosynthesis would have a high potential for peptide engineering by introducing unusual amino acids into desired peptides, which may establish a universal approach to advance the structural design of novel peptides, termed lantibiotic engineering. In this review, we focus on recent development with contemporary innovations and perspective of lantibiotic research.

    DOI: 10.1263/jbb.102.139

  • Lysine-oriented charges trigger the membrane binding and activity of nukacin ISK-1. 査読 国際誌

    Sikder M Asaduzzaman, Jun-Ichi Nagao, Yuji Aso, Jiro Nakayama, Kenji Sonomoto

    Applied and environmental microbiology   72 ( 9 )   6012 - 7   2006年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    The antibacterial activities and membrane binding of nukacin ISK-1 and its fragments and mutants were evaluated to delineate the determinants governing structure-function relationships. The tail region (nukacin(1-7)) and ring region (nukacin(7-27)) were shown to have no antibacterial activity and also had no synergistic effect on each other or even on nukacin ISK-1. Both a fragment with three lysines in the N terminus deleted (nukacin(4-27)) and a mutant with three lysines in the N terminus replaced with alanine (K1-3A nukacin ISK-1) imparted very low activity (32-fold lower than nukacin ISK-1) and also exhibited a similar antagonistic effect on nukacin ISK-1. Addition of two lysine residues at the N terminus (+2K nukacin ISK-1) provided no further increased antibacterial activity. Surface plasmon resonance sensorgrams and kinetic rate constants determined by a BIAcore biosensor revealed that nukacin ISK-1 has remarkably higher binding affinity to anionic model membrane than to zwitterionic model membrane. Similar trends of strong binding responses and kinetics were indicated by the high affinities of nukacin ISK-1 and +2K nukacin ISK-1, but there was no binding of tail region, ring region, nukacin(4-27), and K1-3A nukacin ISK-1 to the anionic model membrane. Our findings therefore suggest that the complete structure of nukacin ISK-1 is necessary for its full activity, in which the N-terminus three lysine residues play a crucial role in electrostatic binding to the target membrane and therefore nukacin ISK-1's ability to exert its potent antibacterial activity.

    DOI: 10.1128/AEM.00678-06

  • Lantibiotics: insight and foresight for new paradigm. 査読

    Jun-ichi Nagao, Sikder M Asaduzzaman, Yuji Aso, Ken-Ichi Okuda, Jiro Nakayama, Kenji Sonomoto

    Journal of bioscience and bioengineering   102 ( 3 )   139 - 49   2006年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Lantibiotics are a unique type of antimicrobial peptide produced by a large number of gram-positive bacteria that contain unusual amino acids, such as lanthionine and dehydrated amino acids. Ribosomally synthesized lantibiotic prepeptide consists of an N-terminal leader peptide followed by a C-terminal propeptide moiety that undergoes several post-translational modification events to yield a biologically active lantibiotic. Research on lantibiotics has drawn much attention in recent years and has undergone extensive progress as a step forward to the next paradigm. Unusual amino acids in lantibiotics solely contribute to their biological activity and also enhance their structural stability. Thus, enzymes involved in lantibiotic biosynthesis would have a high potential for peptide engineering by introducing unusual amino acids into desired peptides, which may establish a universal approach to advance the structural design of novel peptides, termed lantibiotic engineering. In this review, we focus on recent development with contemporary innovations and perspective of lantibiotic research.

    DOI: 10.1263/jbb.102.139

  • Lysine-oriented charges trigger the membrane binding and activity of nukacin ISK-1 査読

    Sikder M. Asaduzzaman, Jun Ichi Nagao, Yuji Aso, Jiro Nakayama, Kenji Sonomoto

    Applied and Environmental Microbiology   72 ( 9 )   6012 - 6017   2006年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    The antibacterial activities and membrane binding of nukacin ISK-1 and its fragments and mutants were evaluated to delineate the determinants governing structure-function relationships. The tail region (nukacin1-7) and ring region (nukacin7-27) were shown to have no antibacterial activity and also had no synergistic effect on each other or even on nukacin ISK-1. Both a fragment with three lysines in the N terminus deleted (nukacin4-27) and a mutant with three lysines in the N terminus replaced with alanine (K1-3A nukacin ISK-1) imparted very low activity (32-fold lower than nukacin ISK-1) and also exhibited a similar antagonistic effect on nukacin ISK-1. Addition of two lysine residues at the N terminus (+2K nukacin ISK-1) provided no further increased antibacterial activity. Surface plasmon resonance sensorgrams and kinetic rate constants determined by a BIAcore biosensor revealed that nukacin ISK-1 has remarkably higher binding affinity to anionic model membrane than to zwitterionic model membrane. Similar trends of strong binding responses and kinetics were indicated by the high affinities of nukacin ISK-1 and +2K nukacin ISK-1, but there was no binding of tail region, ring region, nukacin4-27, and K1-3A nukacin ISK-1 to the anionic model membrane. Our findings therefore suggest that the complete structure of nukacin ISK-1 is necessary for its full activity, in which the N-terminus three lysine residues play a crucial role in electrostatic binding to the target membrane and therefore nukacin ISK-1's ability to exert its potent antibacterial activity.

    DOI: 10.1128/AEM.00678-06

  • Lactococcin Q, a novel two-peptide bacteriocin produced by Lactococcus lactis QU 4. 査読 国際誌

    Takeshi Zendo, Shoko Koga, Yasushi Shigeri, Jiro Nakayama, Kenji Sonomoto

    Applied and environmental microbiology   72 ( 5 )   3383 - 3389   2006年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    A bacteriocin-producing strain, Lactococcus lactis QU 4, was isolated from corn. The bacteriocin, termed lactococcin Q, showed antibacterial activity only against L. lactis strains among a wide range of gram-positive indicator strains tested. Lactococcin Q was purified by acetone precipitation, cation exchange chromatography, and reverse-phase chromatography. Lactococcin Q consisted of two peptides, alpha and beta, whose molecular masses were determined to be 4,260.43 Da and 4,018.36 Da, respectively. Amino acid and DNA sequencing analyses revealed that lactococcin Q was a novel two-peptide bacteriocin, homologous to lactococcin G. Comparative study using chemically synthesized lactococcin Q (Qalpha plus Qbeta) and lactococcin G (Galpha plus Gbeta) clarified that hybrid combinations (Qalpha plus Gbeta and Galpha plus Qbeta) as well as original combinations showed antibacterial activity, although each single peptide showed no significant activity. These four pairs of lactococcin peptides acted synergistically at a 1:1 molar ratio and exhibited identical antibacterial spectra but differed in MIC. The MIC of Qalpha plus Gbeta was 32 times higher than that of Qalpha plus Qbeta, suggesting that the difference in beta peptides was important for the intensity of antibacterial activity.

    DOI: 10.1128/AEM.72.5.3383-3389.2006

  • Purification and amino acid sequence of a bacteriocins produced by Lactobacillus salivarius K7 isolated from chicken intestine

    Komkhae Pilasombut, Thavajchai Sakpuaram, Worawidh Wajjwalku, Sunee Nitisinprasert, Adisorn Swetwiwathana, Takeshi Zendo, Koji Fujita, Jiro Nakayama, Kenji Sonomoto

    Songklanakarin Journal of Science and Technology   28 ( SUPPL. 1 )   121 - 131   2006年3月

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    記述言語:その他   掲載種別:研究論文(その他学術会議資料等)  

    A bacteriocin-producing strain, Lactobacillus K7, was isolated from a chicken intestine. The inhibitory activity was determined by spot-on-lawn technique. Identification of the strain was performed by morphological, biochemical (API 50 CH kit) and molecular genetic (16S rDNA) basis. Bacteriocin purification processes were carried out by amberlite adsorption, cation exchange and reverse-phase high perform ance liquid chromatography. N-terminal amino acid sequences were performed by Edman degradation. Molecular mass was determined by electrospray-ionization (ESI) mass spectrometry (MS). Lactobacillus K7 showed inhibitory activity against Lactobacillus sakei subsp. sakei JCM 1157 , Leuconostoc mesenteroides subsp. mesenteroides JCM 6124 and Bacillus coagulons JCM 2257 . This strain was identified as Lb. salivarius. The antimicrobial substance was destroyed by proteolytic enzymes, indicating its proteinaceous structure designated as a bacteriocin type. The purification of bacteriocin by amberlite adsorption, cation exchange, and reverse-phase chromatography resulted in only one single active peak, which was designated FK22. Molecular weight of this fraction was 4331.70 Da. By amino acid sequence, this peptide was homology to Abp 118 beta produced by Lb. salivarius UCC118. In addition, Lb. salivarius UCC118 produced 2-peptide bacteriocin, which was Abp 118 alpha and beta. Based on the partial amino acid sequences of Abp 118 beta, specific primers were designed from nucleotide sequences according to data from GenBank. The result showed that the deduced peptide was high homology to 2-peptide bacteriocin, Abp 118 alpha and beta. T T T

  • Identification and production of a bacteriocin from Enterococcus mundtii QU 2 isolated from soybean 査読

    Takeshi Zendo, N. Eungruttanagorn, S. Fujioka, Yukihiro Tashiro, K. Nomura, Y. Sera, G. Kobayashi, Jiro Nakayama, A. Ishizaki, Kenji Sonomoto

    Journal of Applied Microbiology   99 ( 5 )   1181 - 1190   2005年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Aims: Identification of the bacteriocin produced by Enterococcus mundtii QU 2 newly isolated from soybean and fermentative production of the bacteriocin. Methods and Results: The bacteriocin produced by Ent. mundtii QU 2 inhibited the growth of various indicator strains, including Enterococcus, Lactobacillus, Leuconostoc, Pediococcus and Listeria. The bacteriocin activity was stable at wide pH range and against heat treatment, but completely abolished by proteolytic enzymes. The bacteriocin was purified from the culture supernatant by the three-step chromatographic procedure. Mass spectrometry, amino acid sequencing and DNA sequencing revealed that the bacteriocin was similar to class IIa bacteriocins produced by other Ent. mundtii strains. The bacteriocin production decreased in the absence of glucose, nitrogen sources, or Tween 80 in MRS medium. Additionally, it was strongly suppressed by addition of Ca 2+ (CaCO3 or CaCl2). In pH-controlled fermentations, the highest bacteriocin production was achieved at pH 6.0 whereas the highest cell growth was obtained at pH 7.0. Conclusions: Ent. mundtii QU 2 produced a class IIa bacteriocin. Some growth factors (e.g. Ca2+ and pH) influenced the bacteriocin production. Significance and Impact of the Study: A new soybean isolate, Ent. mundtii QU 2 was found to be a class IIa bacteriocin producer. Factors influencing the bacteriocin production described herein are valuable for applications of the bacteriocins from Ent. mundtii strains.

    DOI: 10.1111/j.1365-2672.2005.02704.x

  • Lanthionine introduction into nukacin ISK-1 prepeptide by co-expression with modification enzyme NukM in Escherichia coli. 国際誌

    Jun-Ichi Nagao, Yoshitaka Harada, Kouki Shioya, Yuji Aso, Takeshi Zendo, Jiro Nakayama, Kenji Sonomoto

    Biochemical and biophysical research communications   336 ( 2 )   507 - 513   2005年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    We demonstrated lanthionine introduction into hexa-histidine-tagged (His-tagged) nukacin ISK-1 prepeptide NukA by modification enzyme NukM in Escherichia coli. Co-expression of nukA and nukM, purification of the resulting His-tagged prepeptide by affinity chromatography, and subsequent mass spectrometry analysis showed that the prepeptide was converted into a postulated peptide with decrease in mass of 72Da which resulted from dehydration of four amino acids. Characterization of the resultant prepeptide indicated the presence of unusual amino acids, such as dehydrated amino acid, lanthionine or 3-methyllanthionine, in its C-terminal propeptide moiety. The modified prepeptide encompassing the leader peptide attached to the post-translationally modified propeptide moiety was readily obtained by one-step purification. Our findings will thus be a powerful tool for introducing unusual amino acids aimed at peptide engineering and also helpful to provide new insight for further understanding of lanthionine-forming enzymes for lantibiotics.

    DOI: 10.1016/j.bbrc.2005.08.125

  • A novel type of immunity protein, NukH, for the lantibiotic nukacin ISK-1 produced by Staphylococcus warneri ISK-1 査読

    Yuji Aso, Ken Ichi Okuda, Jun Ichi Nagao, Youhei Kanemasa, Nguyen Thi Bich Phuong, Hanako Koga, Kouki Shioya, Toshihiro Sashihara, Jiro Nakayama, Kenji Sonomoto

    Bioscience, Biotechnology and Biochemistry   69 ( 7 )   1403 - 1410   2005年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Staphylococcus warneri ISK-1 produces a lantibiotic, nukacin ISK-1. The nukacin ISK-1 gene cluster consists of at least six genes, nukA, -M, -T, -F, -E, and -G, and two open reading frames, ORF1 and ORF7 (designated nukH). Sequence comparisons suggested that NukF, -E, -G, and -H contribute to immunity to nukacin ISK-1. We investigated the immunity levels of recombinant Lactococcus lactis expressing nukFEG and nukH against nukacin ISK-1. The co-expression of nukFEG and nukH resulted in a high degree of immunity. The expression of either nukFEG or nukH conferred partial immunity against nukacin ISK-1. These results suggest that NukH contributes cooperatively to self-protection with Nuk-FEG. The nukacin ISK-1 immunity system might function against another lantibiotic, lacticin 481. Western blot analysis showed that NukH expressed in Staphylococcus carnosus was localized in the membrane. Peptide release/bind assays indicated that the recombinant L. lactis expressing nukH interacted with nukacin ISK-1 and lacticin 481 but not with nisin A. These findings suggest that NukH contributes cooperatively to host immunity as a novel type of lantibiotic-binding immunity protein with NukFEG.

    DOI: 10.1271/bbb.69.1403

  • Characterization of functional domains of lantibiotic-binding immunity protein, NukH, from Staphylococcus warneri ISK-1. 査読 国際誌

    Ken-ichi Okuda, Yuji Aso, Jun-ichi Nagao, Kouki Shioya, Youhei Kanemasa, Jiro Nakayama, Kenji Sonomoto

    FEMS microbiology letters   250 ( 1 )   19 - 25   2005年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    The immunity to a lantibiotic, nukacin ISK-1, is conferred by NukFEG (ABC transporter) and NukH (lantibiotic-binding protein) cooperatively. The present study identifies the functional domains of NukH. The topological analysis indicated that NukH possesses two external loops and three transmembrane helices. Deletion of N or C terminus of NukH did not affect the function. Amino acids substitutions in the respective loops abolished the function. Deletion of the third transmembrane helix resulted in loss of immunity but did not affect the binding activity. These findings suggested that the whole structure of NukH, except for N and C termini, is essential for its full immunity function, and that NukH inactivates nukacin ISK-1 after binding.

    DOI: 10.1016/j.femsle.2005.06.039

  • Localization and interaction of the biosynthetic proteins for the lantibiotic, nukacin ISK-1 査読

    Jun Ichi Nagao, Yuji Aso, Toshihiro Sashihara, Kouki Shioya, Asaho Adachi, Jiro Nakayama, Kenji Sonomoto

    Bioscience, Biotechnology and Biochemistry   69 ( 7 )   1341 - 1347   2005年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Nukacin ISK-1 is a type-A(II) lantibiotic produced by Staphylococcus warneri ISK-1. In this study, we characterized NukM and NukT, which are predicted to be involved in modification of prepeptide (NukA) and cleavage of leader peptide and subsequent secretion respectively. Localization analysis of NukM and NukT in the wild-type strain indicated that both proteins were located at the cytoplasm membrane. Interestingly, NukM expressed heterologously in St. carnosus TM300 was also located at the cytoplasm membrane even in the absence of NukT. Yeast two-hybrid assay showed that a complex of at least two each of NukM and NukT was associated with NukA. In vitro interaction analysis by surface plasmon resonance biosensor further suggested that membrane-located NukM interacted with NukA. These results indicate that NukM and NukT form a membrane-located multimeric protein complex and that post-translational modification of nukacin ISK-1 would occur at the cytoplasm membrane.

    DOI: 10.1271/bbb.69.1341

  • Characterization of functional domains of lantibiotic-binding immunity protein, NukH, from Staphylococcus warneri ISK-1 査読

    Ken Ichi Okuda, Yuji Aso, Jun Ichi Nagao, Kouki Shioya, Youhei Kanemasa, Jiro Nakayama, Kenji Sonomoto

    FEMS Microbiology Letters   250 ( 1 )   19 - 25   2005年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    The immunity to a lantibiotic, nukacin ISK-1, is conferred by NukFEG (ABC transporter) and NukH (lantibiotic-binding protein) cooperatively. The present study identifies the functional domains of NukH. The topological analysis indicated that NukH possesses two external loops and three transmembrane helices. Deletion of N or C terminus of NukH did not affect the function. Amino acids substitutions in the respective loops abolished the function. Deletion of the third transmembrane helix resulted in loss of immunity but did not affect the binding activity. These findings suggested that the whole structure of NukH, except for N and C termini, is essential for its full immunity function, and that NukH inactivates nukacin ISK-1 after binding.

    DOI: 10.1016/j.femsle.2005.06.039

  • A novel type of immunity protein, NukH, for the lantibiotic nukacin ISK-1 produced by Staphylococcus warneri ISK-1. 査読 国際誌

    Yuji Aso, Ken-ichi Okuda, Jun-ichi Nagao, Youhei Kanemasa, Nguyen Thi Bich Phuong, Hanako Koga, Kouki Shioya, Toshihiro Sashihara, Jiro Nakayama, Kenji Sonomoto

    Bioscience, biotechnology, and biochemistry   69 ( 7 )   1403 - 10   2005年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Staphylococcus warneri ISK-1 produces a lantibiotic, nukacin ISK-1. The nukacin ISK-1 gene cluster consists of at least six genes, nukA, -M, -T, -F, -E, and -G, and two open reading frames, ORF1 and ORF7 (designated nukH). Sequence comparisons suggested that NukF, -E, -G, and -H contribute to immunity to nukacin ISK-1. We investigated the immunity levels of recombinant Lactococcus lactis expressing nukFEG and nukH against nukacin ISK-1. The co-expression of nukFEG and nukH resulted in a high degree of immunity. The expression of either nukFEG or nukH conferred partial immunity against nukacin ISK-1. These results suggest that NukH contributes cooperatively to self-protection with NukFEG. The nukacin ISK-1 immunity system might function against another lantibiotic, lacticin 481. Western blot analysis showed that NukH expressed in Staphylococcus carnosus was localized in the membrane. Peptide release/bind assays indicated that the recombinant L. lactis expressing nukH interacted with nukacin ISK-1 and lacticin 481 but not with nisin A. These findings suggest that NukH contributes cooperatively to host immunity as a novel type of lantibiotic-binding immunity protein with NukFEG.

    DOI: 10.1271/bbb.69.1403

  • Localization and interaction of the biosynthetic proteins for the lantibiotic, Nukacin ISK-1. 査読 国際誌

    Jun-ichi Nagao, Yuji Aso, Toshihiro Sashihara, Kouki Shioya, Asaho Adachi, Jiro Nakayama, Kenji Sonomoto

    Bioscience, biotechnology, and biochemistry   69 ( 7 )   1341 - 7   2005年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Nukacin ISK-1 is a type-A(II) lantibiotic produced by Staphylococcus warneri ISK-1. In this study, we characterized NukM and NukT, which are predicted to be involved in modification of prepeptide (NukA) and cleavage of leader peptide and subsequent secretion respectively. Localization analysis of NukM and NukT in the wild-type strain indicated that both proteins were located at the cytoplasm membrane. Interestingly, NukM expressed heterologously in St. carnosus TM300 was also located at the cytoplasm membrane even in the absence of NukT. Yeast two-hybrid assay showed that a complex of at least two each of NukM and NukT was associated with NukA. In vitro interaction analysis by surface plasmon resonance biosensor further suggested that membrane-located NukM interacted with NukA. These results indicate that NukM and NukT form a membrane-located multimeric protein complex and that post-translational modification of nukacin ISK-1 would occur at the cytoplasm membrane.

    DOI: 10.1271/bbb.69.1341

  • Description of complete DNA sequence of two plasmids from the nukacin ISK-1 producer, Staphylococcus warneri ISK-1. 査読 国際誌

    Yuji Aso, Hanako Koga, Toshihiro Sashihara, Jun-Ichi Nagao, Youhei Kanemasa, Jiro Nakayama, Kenji Sonomoto

    Plasmid   53 ( 2 )   164 - 178   2005年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    We report the whole DNA sequence of two plasmids, pPI-1 (30.2 kb) and pPI-2 (2.8 kb). These plasmids are from Staphylococcus warneri ISK-1, which produces a lantibiotic, nukacin ISK-1. Curing of pPI-1 resulted in a loss of bactericidal activity in the culture supernatant and the host's immunity to nukacin ISK-1, suggesting that the biosynthetic genes of the bacteriocin are encoded by pPI-1. Based on the results of a homology search of each open reading flame, pPI-1 is comprised of the following four distinct regions: (1) the nukacin ISK-1 biosynthesis and immunity gene cluster, (2) the thioredoxin gene cluster, (3) the replication region, and (4) a region of Staphylococcus epidermidis ATCC 12228, highly homologous to pSE-12228-05. Gene organization in the nukacin ISK-1 biosynthesis and immunity gene cluster is different from that in other lacticin-481 type gene clusters. The features of the replication protein encoded in the replicating region are somewhat different from other staphylococcus theta-replicating plasmids. pPI-2 comprised a disinfectant resistant gene, qacC, and the whole DNA sequence showed significant similarity to those of other qacC plasmids such as pSK108, suggesting that pPI-2 belongs to the qacC plasmid group.

    DOI: 10.1016/j.plasmid.2004.08.003

  • Molecular monitoring of the developmental bacterial community in the gastrointestinal tract of Japanese infants. 査読 国際誌

    Prapa Songjinda, Jiro Nakayama, Yumiko Kuroki, Shigemitsu Tanaka, Sanae Fukuda, Chikako Kiyohara, Tetsuro Yamamoto, Kunio Izuchi, Taro Shirakawa, Kenji Sonomoto

    Bioscience, biotechnology, and biochemistry   69 ( 3 )   638 - 41   2005年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    The dynamics of the developmental bacterial community in the Japanese neonatal gastrointestinal tract were examined by monitoring 16S ribosomal RNA gene (rDNA) diversity in fecal samples by PCR and denaturing gradient gel electrophoresis (DGGE). The results showed a certain pattern common in infants without antibiotic treatment, in which aerobes, e.g., Pseudomonas, appeared first and were then immediately replaced by facultative anaerobe, Enterococcus, Streptococcus, and Enterobacteriaceae through the first month, and finally strictly anaerobic Bifidobactrerium appeared.

    DOI: 10.1271/bbb.69.638

  • Reconstitution and function of Tetragenococcus halophila chaperonin 60 tetradecamer. 査読

    Amonlaya Tosukhowong, Jiro Nakayama, Yoshimitsu Mizunoe, Shinya Sugimoto, Daisuke Fukuda, Kenji Sonomoto

    Journal of bioscience and bioengineering   99 ( 1 )   30 - 7   2005年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Tetragenococcus halophila originally isolated from soy sauce is a halophilic lactic acid bacterium which can grow under 4 M sodium chloride. T. halophila chaperonin composed of a core moiety of chaperonin 60 (cpn60) and a lid moiety of chaperonin 10 (cpn10), is thought to contribute to host halotolerant capability. In this report, we reconstituted and characterized the core complex of T. halophila chaperonin by using a recombinant T. halophila cpn60 (Tcpn60) overexpressed in Escherichia coli. The reconstitution of Tcpn60 was performed in the presence of 10 mM MgCl2, 2 mM ATP and 0.8 M (NH4)2SO4 and the resultant oligomer was purified by gel filtration chromatography. Electron microscopy of the reconstituted Tcpn60 revealed a double toroidal tetradecameric structure that is characteristic of bacterial cpn60. The T. halophila tetradecamer cpn60 exhibited an ATPase activity and a refolding activity of both chemically and thermally denatured enolases under wide range of salt concentrations. Furthermore, we demonstrated that heterologous expression of Tcpn60 allowed the normal growth of host Escherichia coli cells under salt stress conditions and this effect was further enhanced by co-expression with Tcpn10. These results suggested that Tcpn60 contributes to the halotolerance property of T. halophila cell as a tetradecamer complex, probably associated with the Tcpn10 complex.

    DOI: 10.1263/jbb.99.30

  • Characterization of a gene cluster of Staphylococcus warneri ISK-1 encoding the biosynthesis of and immunity to the lantibiotic, nukacin ISK-1. 国際誌

    Yuji Aso, Toshihiro Sashihara, Jun-Ichi Nagao, Youhei Kanemasa, Hanako Koga, Taku Hashimoto, Toshimasa Higuchi, Asaho Adachi, Harumi Nomiyama, Ayaaki Ishizaki, Jiro Nakayama, Kenji Sonomoto

    Bioscience, biotechnology, and biochemistry   68 ( 8 )   1663 - 1671   2004年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    We characterized a gene cluster in a plasmid designated pPI-1 of Staphylococcus warneri ISK-1 encoding the biosynthesis of and immunity to the lacticin-481 type lantibiotic, nukacin ISK-1. The DNA sequence suggested that the nukacin ISK-1 gene cluster consists of at least six genes, nukA (a structural gene), -M, -T, -F, -E, -G, and two open reading frames, ORF1 and ORF7. NukM and NukT were predicted to be involved in post-translational modification and secretion of nukacin ISK-1 respectively. NukF, -E, and -G were predicted to form a membrane complex which contributes to self-protection from nukacin ISK-1. Transcriptional analyses revealed that nukM through ORF7 comprises an operon, and that ORF1 is transcribed independently from downstream of nukA. The transcriptional levels of the nukA and nukM genes were enhanced by osmotic stress. The expression level of the nukA transcript was scarcely enhanced by nukacin ISK-1, suggesting that expression is not under the control of the autoregulatory circuit.

    DOI: 10.1271/bbb.68.1663

  • Heterologous expression and functional analysis of the gene cluster for the biosynthesis of and immunity to the lantibiotic, nukacin ISK-1 査読

    Yuji Aso, Jun Ichi Nagao, Hanako Koga, Ken Ichi Okuda, Youhei Kanemasa, Toshihiro Sashihara, Jiro Nakayama, Kenji Sonomoto

    Journal of Bioscience and Bioengineering   98 ( 6 )   429 - 436   2004年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Nukacin ISK-1 is a lantibiotic produced by Staphylococcus warneri ISK-1. The gene cluster of nukacin ISK-1 consists of at least nukAMTFEG, ORF1 and ORF7. In this study, we demonstrated the heterologous production of nukacin ISK-1 in Lactococcus lactis by the artificial polycistronic expression of nukAMTFEG-ORF7 under the control of the nisin-controlled expression (NICE) system. Consequently, the recombinant L. lactis showed antimicrobial activity. Mass analysis clarified the presence of nukacin ISK-1 produced in the culture supernatant. These results suggested that the recombinant L. l.actis produced nukacin ISK-1 heterologously. Inactivation of nukA, -M or -T resulted in the complete loss of the nukacin ISK-1 production phenotype. This finding suggested that nukAMT are indispensably associated with the biosynthesis of nukacin ISK-1. To our knowledge, this is the first report of the heterologous production of lantibiotic using the NICE system.

    DOI: 10.1016/S1389-1723(05)00308-7

  • The two-synergistic peptide bacteriocin produced by Enterococcus faecium NKR-5-3 isolated from Thai fermented fish (Plara). 査読 国際誌

    Wilaipun, P., T. Zendo, M. Sangjindavong, S. Nitisinprasert, V. Leelawatcharamas, J. Nakayama, and K. Sonomoto

    ScienceAsia   - 30   2004年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • Nucleotide sequences of genes encoding allosamidin-sensitive and -insensitive chitinases produced by allosamidin-producing Streptomyces. 査読 国際誌

    Hiroshi Matsuura, Susumu Okamoto, Sarintip Anamnart, Qiuqi Wang, Ze-Yang Zhou, Takuya Nihira, Yasuhiro Yamada, Tomohisa Kuzuyama, Haruo Seto, Jiro Nakayama, Akinori Suzuki, Hiromichi Nagasawa, Shohei Sakuda

    Bioscience, biotechnology, and biochemistry   67 ( 9 )   2002 - 2005   2003年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Allosamidin is a strong inhibitor of family 18 chitinases. We previously reported the presence of allosamidin-sensitive and -insensitive chitinases (chitinase S and IS) in the culture filtrate of the allosamidin-producing strain, Streptomyces sp. AJ9463. In this study, we cloned and sequenced the genes encoding the two chitinases, which clarified that chitinase S and IS belong to the family 18 and 19 chitinase, respectively.

    DOI: 10.1271/bbb.67.2002

  • Identification of the lantibiotic nisin Q, a new natural nisin variant produced by Lactococcus lactis 61-14 isolated from a river in Japan. 査読 国際誌

    Takeshi Zendo, Masanori Fukao, Kyoko Ueda, Tomoko Higuchi, Jiro Nakayama, Kenji Sonomoto

    Bioscience, biotechnology, and biochemistry   67 ( 7 )   1616 - 9   2003年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Lactococcus lactis 61-14 isolated from river water produced a bacteriocin active against a wide range of Gram-positive bacteria. N-terminal amino acid sequencing, mass spectral analysis of the purified bacteriocin, and genetic analysis using nisin-specific primers showed that the bacteriocin was a new natural nisin variant, termed nisin Q. Nisin Q and nisin A differ in four amino acids in the mature peptide and two in the leader sequence.

    DOI: 10.1271/bbb.67.1616

  • Effect of heterologous expression of molecular chaperone DnaK from Tetragenococcus halophilus on salinity adaptation of Escherichia coli 査読

    Shinya Sugimoto, Jiro Nakayama, Daisuke Fukuda, Shino Sonezaki, Maki Watanabe, Amonlaya Tosukhowong, Kenji Sonomoto

    Journal of Bioscience and Bioengineering   96 ( 2 )   129 - 133   2003年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Molecular chaperone DnaK of halophilic Tetragenococcus halophilus JCM5888 was characterized under salinity conditions both in vitro and in vivo. The dnaK gene was cloned into an expression vector and transformed into Escherichia coli. The DnaK protein obtained from the recombinant E. coli showed a significantly higher refolding activity of denatured lactate dehydrogenase than that from non-halophilic Lactococcus lactis under NaCl concentrations higher than 1 M. E. coil without the overexpression of DnaK exhibited a growth profile with a prolonged lag phase and suppressed maximum cell density in Luria-Bertani medium containing 5% (0.86 M) NaCl. On the contrary, the overexpression of T. halophilus DnaK greatly shortened this prolonged lag phase with no effect on maximum growth, while that of L. lactis DnaK decreased maximum growth. The amount of protein aggregates was increased by salt stress in the E. coli cells, while this aggregation was greatly suppressed by the overexpression of T. halophilus DnaK. These results suggest that heterologous overexpression of T. halophilus DnaK, via its chaperone activity, promotes salinity adaptation of E. coli.

    DOI: 10.1016/S1389-1723(03)90114-9

  • Identification of the lantibiotic nisin q, a new natural nisin variant produced by lactococcus lactis 61-14 isolated from a river in japan 査読

    Takeshi Zendo, Masanori Fukao, Kyoko Ueda, Tomoko Higuchi, Jiro Nakayama, Kenji Sonomoto

    Bioscience, Biotechnology and Biochemistry   67 ( 7 )   1616 - 1619   2003年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Lactococcus lactis 61-14 isolated from river water produced a bacteriocin active against a wide range of Gram-positive bacteria. N-terminal amino acid sequencing, mass spectral analysis of the purified bacteriocin, and genetic analysis using nisin-specific primers showed that the bacteriocin was a new natural nisin variant, termed nisin Q. Nisin Q and nisin A differ in four amino acids in the mature peptide and two in the leader sequence.

    DOI: 10.1271/bbb.67.1616

  • Aminoacylation of the N-terminal cysteine is essential for Lol-dependent release of lipoproteins from membranes but does not depend on lipoprotein sorting signals. 国際誌

    Ayumu Fukuda, Shin-Ichi Matsuyama, Takashi Hara, Jiro Nakayama, Hiromichi Nagasawa, Hajime Tokuda

    The Journal of biological chemistry   277 ( 45 )   43512 - 43518   2002年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Lipoproteins are present in a wide variety of bacteria and are anchored to membranes through lipids attached to the N-terminal cysteine. The Lol system of Escherichia coli mediates the membrane-specific localization of lipoproteins. Aspartate at position 2 functions as a Lol avoidance signal and causes the retention of lipoproteins in the inner membrane, whereas lipoproteins having residues other than aspartate at position 2 are released from the inner membrane and localized to the outer membrane by the Lol system. Phospholipid:apolipoprotein transacylase, Lnt, catalyzes the last step of lipoprotein modification, converting apolipoprotein into mature lipoprotein. To reveal the importance of this aminoacylation for the Lol-dependent membrane localization, apolipoproteins were prepared by inhibiting lipoprotein maturation. Lnt was also purified and used to convert apolipoprotein into mature lipoprotein in vitro. The release of these lipoproteins was examined in proteoliposomes. We show here that the aminoacylation is essential for the Lol-dependent release of lipoproteins from membranes. Furthermore, lipoproteins with aspartate at position 2 were found to be aminoacylated both in vivo and in vitro, indicating that the lipoprotein-sorting signal does not affect lipid modification.

    DOI: 10.1074/jbc.M206816200

  • Functional analysis of TraA, the sex pheromone receptor encoded by pPD1, in a promoter region essential for the mating response in Enterococcus faecalis. 国際誌

    Takaaki Horii, Hiromichi Nagasawa, Jiro Nakayama

    Journal of bacteriology   184 ( 22 )   6343 - 50   2002年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Conjugative transfer of a bacteriocin plasmid, pPD1, of Enterococcus faecalis is induced in response to a peptide sex pheromone, cPD1, secreted from plasmid-free recipient cells. cPD1 is taken up by a pPD1 donor cell and binds to an intracellular receptor, TraA. Once a recipient cell acquires pPD1, it starts to produce an inhibitor of cPD1, termed iPD1, which functions as a TraA antagonist and blocks self-induction in donor cells. In this study, we discuss how TraA transduces the signal of cPD1 to the mating response. Gel mobility shift assays indicated that TraA is bound to a traA-ipd intergenic region, which is essential for cPD1 response. DNase I footprinting analysis suggested the presence of one strong (tab1) and two weak (tab2 and tab3) TraA-binding sites in the intergenic region. Primer extension analysis implied that the transcriptional initiation sites of traA and ipd were located in the intergenic region. Northern analysis showed that cPD1 upregulated and downregulated transcription of ipd and traA, respectively. The circular permutation assay showed that TraA bent a DNA fragment corresponding to the tab1 region, and its angle was changed in the presence of cPD1 or iPD1. From these data, we propose a model that TraA changes the conformation of the tab1 region in response to cPD1 and upregulates the transcription of ipd, which may lead to expression of genes required for the mating response.

    DOI: 10.1128/JB.184.22.6343-6350.2002

  • Blasticidin A as an inhibitor of aflatoxin production by Aspergillus parasiticus 査読

    S. Sakuda, M. Ono, H. Ikeda, T. Nakamura, Y. Inagaki, R. Kawachi, Jiro Nakayama, A. Suzuki, A. Isogai, H. Nagasawa

    Journal of Antibiotics   53 ( 11 )   1265 - 1271   2000年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Blasticidin A, an antibiotic, showed strong inhibitory activity toward aflatoxin production by Aspergillus parasiticus. Its structure was characterized by NMR and chemical degradation experiments as 1, which is a tetramic acid derivative with a highly oxygenated long alkyl chain similar to aflastatin A (2). Absolute configurations of the eight chiral centers at C-4, 6, 31, 32, 33, 34, 35 and 37 of 1 were chemically determined. Blasticidin A almost completely inhibited aflatoxin production at 0.5 μm.

    DOI: 10.7164/antibiotics.53.1265

  • Aflastatins: New streptomyces metabolites that inhibit aflatoxin biosynthesis

    Shohei Sakuda, Makoto Ono, Hiroyuki Ikeda, Masaru Sakurada, Jiro Nakayama, Akinori Suzuki, Akira Isogai

    Biologically Active Natural Products: Agrochemicals   185 - 200   1999年1月

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    記述言語:その他  

    Aflatoxins are a group of mycotoxins produced by some strains of the fungi, Aspergillus parasiticus, Aspergillus flavus, and Aspergillus nomius. These aflatoxin-producing fungi are present ubiquitously in the world, but they don’t always produce the toxin. Under some environmental conditions of high temperature and humidity, especially at tropical or subtropical zones, they infect agricultural products such as peanuts or corn, and produce aflatoxins not only on the outside, but also on the inside. Aflatoxins were first found in 1960 as toxic metabolites produced by A. flavus which killed numerous turkeys in England. They also were shown to have an extremely potent carcinogenicity toward mammals and found as contaminates in a wide variety of food commodities. Aflatoxin is now generally recognized not only as an extremely toxic contaminant in foods and feeds, but also as one of the certain risk factors for liver cancer in humans. 1 Thus, control and management of aflatoxins have become issues of concern.

  • Structures and biosynthesis of aflastatins: Novel inhibitors of aflatoxin production by Aspergillus parasiticus

    Makoto Ono, Shohei Sakuda, Hiroyuki Ikeda, Kazuo Furihata, Jiro Nakayama, Akinori Suzuki, Akira Isogai

    Journal of Antibiotics   51 ( 11 )   1019 - 1028   1998年11月

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    記述言語:その他   掲載種別:研究論文(学術雑誌)  

    Two novel inhibitors of aflatoxin production by Aspergillus parasiticus were isolated from the mycelial extracts of Streptomyces sp. MRI142 and termed aflastatin A and B. The structures of aflastatin A (1) and B (5) were elucidated by NMR and chemical degradation experiments. These compounds have a novel skeleton of a tetramic acid derivative with a highly oxygenated long alkyl chain. The incorporation experiments using C-labeled acetates, propionate, glucose and glycolate suggested that most of the C and C units involved in the alkyl chain moiety of aflastatin A were biosynthesized from acetic and propionic acids, but five C units in the alkyl chain originated from glycolic acid. 13 2 3 2

    DOI: 10.7164/antibiotics.51.1019

  • Molecular mechanism of peptide-specific pheromone signaling in Enterococcus faecalis: Functions of pheromone receptor TraA and pheromone- binding protein TraC encoded by plasmid pPD1 査読

    Jiro Nakayama, Yuuichiro Takanami, Takaaki Horii, Shohei Sakuda, Akinori Suzuki

    Journal of Bacteriology   180 ( 3 )   449 - 456   1998年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Conjugative transfer of the Enterococcus faecalis plasmid pPD1 is activated by cPD1, one of several peptide sex pheromones secreted by plasmid- free recipient cells, and is blocked by a donor-produced peptide inhibitor, iPD1. Using a tritiated pheromone, [ H]cPD1, we investigated how pPD1- harboring donor cells receive these peptide signals. Donor cells rapidly incorporated [ H]cPD1. The cell extract but not the membrane fraction of the donor strain exhibited significant [ H] cPD1-binding activity. On the basis of these data and those of tracer studies, it was demonstrated that cPD1 was internalized, where it bound to a high-molecular-weight compound. The cell extract of a strain carrying the traA-bearing multicopy plasmid (pDLHH21) also exhibited high [ H]cPD1-binding activity. A recombinant TraA exhibited a dissociation constant of 0.49 ± 0.08 nM against [ H]cPD1. iPD1 competitively inhibited [ H]cPD1 binding to TraA, whereas pheromones and inhibitors relating to other plasmid systems did not. These results show that TraA is a specific intracellular receptor for cPD1 and that iPD1 acts as an antagonist for TraA. A strain carrying the traC-bearing multicopy plasmid (pDLES23) exhibited significant [ H]cPD1-binding activity. A strain carrying traC-disrupted pPD1 (pAM351CM) exhibited lower [ H]cPD1-binding activity as well as lower sensitivity to cPD1 than a wild-type donor strain. Some of the other pheromones and inhibitors inhibited [ H]cPD1 binding to the traC transformant like cPD1 and iPD1 did. These results show that TraC, as an extracellular less-specific pheromone-binding protein, supports donor cells to receive cPD1. 3 3 3 3 3 3 3 3 3

    DOI: 10.1128/jb.180.3.449-456.1998

  • Structures and biosynthesis of aflastatins: novel inhibitors of aflatoxin production by Aspergillus parasiticus 査読

    Makoto Ono, Shohei Sakuda, Hiroyuki Ikeda, Kazuo Furihata, Jiro Nakayama, Akinori Suzuki, Akira Isogai

    Journal of Antibiotics   51 ( 11 )   1019 - 1028   1998年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Two novel inhibitors of aflatoxin production by Aspergillus parasiticus were isolated from the mycelial extracts of Streptomyces sp. MRI142 and termed aflastatin A and B. The structures of aflastatin A (1) and B (5) were elucidated by NMR and chemical degradation experiments. These compounds have a novel skeleton of a tetramic acid derivative with a highly oxygenated long alkyl chain. The incorporation experiments using 13C-labeled acetates, propionate, glucose and glycolate suggested that most of the C2 and C3 units involved in the alkyl chain moiety of aflastatin A were biosynthesized from acetic and propionic acids, but five C2 units in the alkyl chain originated from glycolic acid.

    DOI: 10.7164/antibiotics.51.1019

  • Structure of blasticidin A 査読

    Shohei Sakuda, Ono Makoto, Hiroyuki Ikeda, Yasuhito Inagaki, Jiro Nakayama, Akinori Suzuki, Akira Isogai

    Tetrahedron Letters   38 ( 42 )   7399 - 7402   1997年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    The structure of blasticidin A was characterized as 1, which is a tetramic acid derivative with a highly oxygenated long alkyl chain similar to aflastatin A 2.

    DOI: 10.1016/S0040-4039(97)01734-6

  • Genetic analysis of plasmid-specific pheromone signaling encoded by ppd1 in enterococcus faecalis 査読

    Jiro Nakayama, Akinori Suzuki

    Bioscience, Biotechnology and Biochemistry   61 ( 11 )   1796 - 1799   1997年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Certain plasmids in Enterococcus faecalis encode a mating response to recipient-produced peptide sex pheromones. Targeted disruption of tra genes on pPD1 suggested that TraA plays a central role in the plasmid-specific pheromone signaling pathway. TraA functioned as a negative regulator for the pheromone-inducible conjugal transfer. Complementation analysis of pPD1 tra gene mutants by pAD1 suggested that the pheromone binding function of TraC was non-specific between these plasmids, but the function of TraA and the pheromone shutdown function of TraB are plasmid-specific. © 1997, Taylor & Francis Group, LLC. All rights reserved.

    DOI: 10.1271/bbb.61.1796

  • Aflastatin A, a novel inhibitor of aflatoxin production of Aspergillus parasiticus, from Streptomyces 査読

    Shohei Sakuda, Makoto Ono, Kazuo Furihata, Jiro Nakayama, Akinori Suzuki, Akira Isogai

    Journal of the American Chemical Society   118 ( 33 )   7855 - 7856   1996年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1021/ja960899d

  • Isolation and Structure of staph-cAM373 Produced by Staphylococcus aureus That Induces Conjugal Transfer of Enterococcus faecalis Plasmid pAM373 査読

    Liro Nakayama, Shigeru Igarashi, Hiromichi Nagasawa, Don B. Clewell, Florence Y. An, Akinori Suzuki

    Bioscience, Biotechnology and Biochemistry   60 ( 6 )   1038 - 1039   1996年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Enterococus faecalis plasmid pAM373 encodes a mating response to the sex pheromone, cAM373, which is secreted from pAM373-free E. faecalis. cAM373-like activity was detected in a culture filtrate of Staphylococcus aureus. The major active substance, termed staph-cAM313 was isolated, and its structure was identified as a H-Ala-Ile-Phe-Ile-Leu-Ala-Ala-OH heptapeptide. © 1996, Taylor & Francis Group, LLC. All rights reserved.

    DOI: 10.1271/bbb.60.1038

  • Quantitative analysis for pheromone inhibitor and pheromone shutdown in Enterococcus faecalis. 査読

    Jiro Nakayama, G. M. Dunny, D. B. Clewell, A. Suzuki

    Developments in Biological Standardization   85   35 - 38   1995年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • Structural Analysis of New Syringopeptins by Tandem Mass Spectrometry 査読

    Akira Isogai, Akira Isogai, Jiro Nakayama, Jiro Nakayama, Akinori Suzuki, Akihiko Kusai, Jon Y. Takemoto

    Bioscience, Biotechnology and Biochemistry   59 ( 7 )   1374 - 1376   1995年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    New syringopeptins SP(SC)-1 and -2 were isolated from culture filtrates of phytopathogenic bacterium strain SC1 of Pseudomonas syringae pv. syringae. These syringopeptins were composed of a β-hydroxy fatty acid, a long sequence of aliphatic amino acids, and a lactone moiety of eight amino acids. The amino acid sequences were deduced from a comparison of their tandem mass sepctra with those of known syringopeptins SP-22a and SP-25a. SP(SC)-1 and SP(SC)-2 resembled SP-22a, but differed from the latter by 3 amino acids.

    DOI: 10.1271/bbb.59.1374

  • Rotihibins, Novel Plant Growth Regulators from Streptomyces graminofaciens 査読

    Naoyuki Fukuchi, Kazuo Furihata, Jiro Nakayama, Akira Isogai

    Journal of Antibiotics   48 ( 9 )   1004 - 1010   1995年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    In the course of screening search for plant growth regulators, a culture filtrate of Streptomyces graminofaciens 3C02 was found to inhibit the growth of lettuce seedlings. The active substances, named rotihibin A (1) and B (2), were revealed to be lipo-peptidal compounds. Rotihibins inhibit growth of various plants at below 1 μg/ml, but do not show lethal activity even at higher doses.

    DOI: 10.7164/antibiotics.48.1004

  • Structure and stereochemistry of three phytotoxins, syringomycin, syringotoxin and syringostatin, produced by Pseudomonas syringae pv. syringae 査読

    Naoyuka Fukuchi, Akira Isogai, Jiro Nakayama, Seiji Takayama, Shuichi Yamashita, Kazuo Suyama, Jon Y. Takemoto, Akinori Suzuki

    Journal of the Chemical Society, Perkin Transactions 1   ( 9 )   1149 - 1157   1992年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    The structures of two phytotoxins, syringomycin and syringotoxin, produced by Pseudomonas syringae pv. syringae, were determined. Several amino acid residues of syringomycin were different from those in the syringostatins. Syringotoxin B proved to be [Gly3]syringostatin A. The three kinds of phytotoxins showed close structural similarity, and the stereochemistry of their components was deduced and compared.

  • Partial amino-acid sequence of ECP31, a carrot embryogenic-cell protein, and enhancement of its accumulation by abscisic acid in somatic embryos 査読

    Tomohiro Kiyosue, Jiro Nakayama, Shinobu Satoh, Akira Isogai, Akinori Suzuki, Hiroshi Kamada, Hiroshi Harada

    Planta   186 ( 3 )   337 - 342   1992年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    ECP31, an embryogenic-cell protein from carrot (Daucus carota L.), was purified by sequential column-chromatographic steps and digested by V8 protease on a nitrocellulose membrane. The resultant peptides were separated by reverse-phased column chromatography and sequenced. The sequences obtained were 70-80% homologous to those of a late-embryogenesis-abundant protein (D34) from cotton (Baker et al, 1988, Plant Mol. Biol. 11, 227-291). The level of ECP31 in somatic embryos of carrot was increased by treatment of the embryos with 3.7 · 10 M abscisic acid (ABA) for 48 h, and there was no change in this enhanced level for up to 192 h in the presence of ABA. No similar enhancing effect of ABA was observed on the level of ECP31 in embryogenic callus or segments of carrot hypocotyls. In an immunohistochemical analysis, ECP31 was found in epidermal tissue and in the vascular system of ABA-treated somatic embryos. © 1992 Springer-Verlag. -6

    DOI: 10.1007/BF00195313

  • Isolation and structural elucidation of syringostatins, phytotoxins produced by pseudomonas syringae pv. syringae lilac isolate 査読

    Naoyuki Fukuchi, Akira Isogai, Jiro Nakayama, Seiji Takayama, Shuichi Yamashita, Kazuo Suyama, Akinori Suzuki

    Journal of the Chemical Society, Perkin Transactions 1   ( 7 )   875 - 880   1992年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    A bacterial strain of Pseudomonas syringae pv. syringae isolated from lilac was found to produce a homologous mixture of phytotoxins different from syringomycin and syringotoxin. The toxins were termed syringostatins and the structures of the main components, syringostatins A and B, were determined by 2D-NMR spectroscopy and mass spectrometry. Minor component structures were elucidated from mass/mass spectra.

  • Structural Elucidation of Rotihibin B by Tandem Mass Spectrometry 査読

    Naoyuki Fukuchi, Jiro Nakayama, Seiji Takayama, Akira Isogai, Akinori Suzuki

    Bioscience, Biotechnology and Biochemistry   56 ( 7 )   1152 - 1153   1992年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1271/bbb.56.1152

  • Structural Elucidation of Minor Components of Peptidyl Antibiotic P168s (Leucinostatins) by Tandem Mass Spectrometry 査読

    Akira Isogai, Jiro Nakayama, Seiji Takayama, Akinori Suzuki, Akihiko Kusai

    Bioscience, Biotechnology and Biochemistry   56 ( 7 )   1079 - 1085   1992年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Tandem mass spectrometry with a four-sector type mass spectrometer was used to elucidate the structures of minor components of the peptidyl antibiotic P168s (leucinostatins). As N-terminal fragments, ions by B-type cleavage were dominant, while V-type cleavages were observed along with X, Y, and Z types as C-terminal ions. The V-type ions were predominant in the cleavages of the amino terminals of leucyl and hydroxyleucyl residues. The structures of several minor components could be deduced from the tandem mass spectra.

    DOI: 10.1271/bbb.56.1079

  • Expression of a Putative Precursor of Pheromone Biosynthesis Activating Neuropeptide-I (PBAN-I) of the Silkmoth, Bombyx mori, and Its Conversion to the Mature Peptide, PBAN-I 査読

    Tsuyoshi Kawano, Hisato Kuniyoshi, Jiro Nakayama, Hiromichi Nagasawa, Akinori Suzuki

    Bioscience, Biotechnology and Biochemistry   55 ( 7 )   1943 - 1945   1991年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1271/bbb1961.55.1943

  • Amino acid sequence of pheromone-inducible surface protein in Enterococcus faecalis, that is encoded on the conjugative plasmid pPD1 査読

    Jiro Nakayama, Hiromich Nagasawa, Akira Isogai, Don B. Clewell, Akinori Suzuki

    FEBS Letters   268 ( 1 )   245 - 248   1990年7月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    The major pheromone-inducible protein, PD78, believed to contribute to bacterial conjugation, was purified from Enterococcus (formerly Streptococcus) faecalis cells containing the plasmid pPD1. A cloned EcoRI-Bg1II 3.6-kbp fragment of the plasmid pAM351(pPd1::Tn916) contained an open reading frame corresponding to 467 amino acid residues representing PD78. In a central region of the deduced protein, there is a repeated sequence of X-X-Pro that is repeated 15 times. This is analogous to the Gin-Gin-Pro repeat in the C-tenninal region of TraD product encoded on the R 100 plasmid in Escherichia coli. © 1990.

    DOI: 10.1016/0014-5793(90)81019-K

  • Structure of Syringotoxin B, a Phytotoxin Produced by Citrus Isolates of Pseudomonas syringae pv. syringae 査読

    Naoyuki Fukuchi, Akira Isogai, Jiro Nakayama, Akinori Suzuki

    Bioscience, Biotechnology and Biochemistry   54 ( 12 )   3377 - 3379   1990年1月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1271/bbb1961.54.3377

  • Stucture of Demethylallosamidin as an Insect Chitinase Inhibitor 査読

    Akira Isogai, Masahiro Sato, Shohei Sakuda, Jiro Nakayama, Akinori Suzuki

    Agricultural and Biological Chemistry   53 ( 10 )   2825 - 2826   1989年10月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1080/00021369.1989.10869709

▼全件表示

書籍等出版物

  • 腸内フローラの形成と疾患ー食・栄養・くすりがどのように関わるのか?ー

    中山二郎(担当:共著)

    医薬出版  2018年8月 

     詳細を見る

    担当ページ:アジア マイクロビオーム プロジェクト:アジア人の食と健康のインターフェースとしての腸内フローラの理解に向けて   記述言語:日本語   著書種別:学術書

  • 食と微生物の事典

    山本麻寿紗, 田中優,中山二郎(担当:共著)

    朝倉書店  2017年6月 

     詳細を見る

    担当ページ:4.17腸内微生物の年齢による変化   記述言語:日本語   著書種別:学術書

  • 食と微生物の事典

    本田倫子,中山二郎(担当:共著)

    朝倉書店  2017年6月 

     詳細を見る

    担当ページ:4.18 腸内微生物の年齢による変化   記述言語:日本語   著書種別:学術書

  • 応用微生物学

    中山 二郎(担当:共著)

    文永堂出版  2016年7月 

     詳細を見る

    担当ページ:第9章   記述言語:日本語   著書種別:一般書・啓蒙書

  • ヒトマイクロバイオーム研究最前線(第1編第3節)アジア人の腸内細菌叢比較

    本田倫子,中山二郎(担当:共著)

    NTS  2016年3月 

     詳細を見る

    担当ページ:第1編第3節   記述言語:日本語   著書種別:学術書

  • Microbial Biofilms

    中山 二郎, 庄島あかね, Gianfranco Donelli(担当:共著)

    Springer  2014年9月 

     詳細を見る

    記述言語:英語   著書種別:学術書

  • 乳酸菌とビフィズス菌のサイエンス

    山本憲二、中山二郎、他(担当:共著)

    京都大学学術出版会  2010年11月 

     詳細を見る

    記述言語:日本語   著書種別:一般書・啓蒙書

  • Comprehensive Natural Products II: Chemistry and Biologym, Cell-to-cell communications among microorganisms

    Sueharu Horinouchi, Kenji Ueda, Jiro Nakayama, Tsukasa Ikeda(担当:共著)

    Elsevier  2010年3月 

     詳細を見る

    担当ページ:Vol.4, 283-337   記述言語:英語   著書種別:学術書

  • Effect of garlic on lactic acid and bacteriocins production of bacteriocin-producing lactic acid bacteria associated in Nham (Thai fermented meat) during fermentation

    Swetwiwathana A., Lotong N., Nakayama J., Sonomoto K.

    52nd International Congress of Meat Science and Technology: Harnessing and exploiting global opportunities  2023年8月    ISBN:9789086865796, 9789086860104

     詳細を見る

  • 食品免疫学辞典

    中山二郎(担当:共著)

    朝倉書店  2021年11月 

     詳細を見る

    担当ページ:Actinobacteria; p 216 - p.217   記述言語:日本語   著書種別:学術書

  • 食品免疫学辞典

    中山二郎(担当:共著)

    朝倉書店  2021年11月 

     詳細を見る

    担当ページ:Bacteroides (p.214 - p.215)   記述言語:日本語   著書種別:学術書

  • Biotechnological Applications of Quorum Sensing Inhibitors

    Basit Yousuf, Keika Adachi, Jiro Nakayama(担当:共著)

    Springer Nature  2018年6月 

     詳細を見る

    担当ページ:Chapter 9. Developing anti-virulence chemotherapies by exploiting the diversity of mocirobial quorum sensing systems   記述言語:英語   著書種別:学術書

  • Quorum quenching strategy targeting gram-positive pathogenic bacteria

    Ravindra Pal Singh, Said E. Desouky, Jiro Nakayama

    Springer New York LLC  2016年1月 

     詳細を見る

    記述言語:英語  

    Quorum sensing (QS) is a cell density-dependent regulatory system that orchestrates the group behavior of unicellular organisms by synchronizing the expression of certain gene(s) within the clonal community of same species. Bacterial pathogens often employ QS system to establish efficiently an infection. A large part of low GC Gram-positive bacteria belonging to phylum Firmicutes use thiolactone/lactone peptides as communication signals so-called autoinducing peptides (AIPs) to coordinate QS circuit. In particular, QS of staphylococci, enterococci, and clostridia have been intensively studied in terms of alternative target of antipathogenic chemotherapy independent of bactericidal antibiotics. Thus far, a number of quorum quenching (QQ) agents that targeting the QS circuit of these Gram-positive pathogens have been developed by random screening of natural compounds or rationale design of AIP antagonists. This review summarizes those QQ agents and previews their potential as post-antibiotic drugs.

    DOI: 10.1007/5584_2016_1

  • Quorum Sensing vs Quorum Quenching: A Battle with no end in Sight (Development of quorum-sensing inhibitors targeting the fsr system of enterococcus faecalis)

    Ravindra Pal Singh, Jiro Nakayama(担当:共著)

    Springer  2015年1月 

     詳細を見る

    担当ページ:319-326   記述言語:英語   著書種別:学術書

    Enterococcus spp. can cause illnesses such as bacteremia, endocarditis, urinary tract infections, posttreatment endophthalmitis, and endodontic infections (Murray 2000; Marothi et al. 2005). Medical treatment of these infectious diseases depends largely on bactericidal or bacteriostatic antibiotics. However, frequent use of such antibiotics has led to the development of drug-resistant bacterial strains, which are difficult to treat (Marothi et al. 2005; Murray 2000). As a result, the blockage of bacterial quorum-sensing (QS) systems has attracted attention owing to its potential to attenuate bacterial virulence without inducing bactericidal pressures that lead to drug resistance. This approach, called quorum quenching (QQ), can be undertaken partially or completely independently of antibiotic treatment. Among several QQ strategies, the use of QS inhibitors (QSIs), which are small molecules that have no adverse effects on bacteria, offers advantages in terms of drug delivery and decreased damage to commensal microbiota.

    DOI: 10.1007/978-81-322-1982-8__25

  • Quorum Sensing vs Quorum Quenching: A Battle with no end in Sight (Quorum-sensing systems in enterococci)

    Ravindra Pal Singh, Jiro Nakayama(担当:共著)

    Springer  2015年1月 

     詳細を見る

    担当ページ:155-163   記述言語:英語   著書種別:学術書

    Enterococcus is a genus of Gram-positive bacteria that is ubiquitous in natural ecosystems, plants, and animals. Some species of Enterococcus are present in the normal gastrointestinal bacterial community. However, others notably Enterococcus faecalis and Enterococcus faecium often cause opportunistic infections such as bacteremia, endocarditis, urinary tract infections, posttreatment endodontic infections, and endophthalmitis (Arias et al. 2010). Bacteria often use quorum sensing (QS) systems to control the expression of certain virulence genes and establish infection efficiently (Waters and Bassler 2005).

    DOI: 10.1007/978-81-322-1982-8__14

  • 環境と微生物の事典

    中山二郎、他(担当:共著)

    朝倉書店  2014年5月 

     詳細を見る

    記述言語:日本語   著書種別:学術書

  • 世紀を越えるビフィズス菌の研究

    上野川修一、山本憲二、中山二郎、他(担当:共著)

    (財)日本ビフィズス菌センター  2011年6月 

     詳細を見る

    記述言語:日本語   著書種別:学術書

  • 腸内共生系のバイオサイエンス

    上野川修一、清野宏、中山二郎、他(担当:共著)

    丸善出版  2011年5月 

     詳細を見る

    記述言語:日本語   著書種別:学術書

  • Cell-to-cell communications among microorganisms

    Sueharu Horinouchi, Kenji Ueda, Jiro Nakayama, Tsukasa Ikeda

    Elsevier Ltd  2010年3月 

     詳細を見る

    担当ページ:4, 283-337   記述言語:英語  

  • 医科プロバイオティクス学

    中山二郎、他(担当:共著)

    シナジー  2009年1月 

     詳細を見る

    記述言語:日本語   著書種別:一般書・啓蒙書

  • プロバイオティクスとバイオジェニクス

    中山二郎,田中重光,園元謙二(担当:共著)

    NTS  2005年1月 

     詳細を見る

    担当ページ:第4節2.p311-322   記述言語:日本語   著書種別:学術書

  • Aflastatins New streptomyces metabolites that inhibit aflatoxin biosynthesis

    Shohei Sakuda, Makoto Ono, Hiroyuki Ikeda, Masaru Sakurada, Jiro Nakayama, Akinori Suzuki, Akira Isogai

    CRC Press  1999年1月 

     詳細を見る

    担当ページ:185-200   記述言語:英語  

    Aflatoxins are a group of mycotoxins produced by some strains of the fungi, Aspergillus parasiticus, Aspergillus flavus, and Aspergillus nomius. These aflatoxin-producing fungi are present ubiquitously in the world, but they don’t always produce the toxin. Under some environmental conditions of high temperature and humidity, especially at tropical or subtropical zones, they infect agricultural products such as peanuts or corn, and produce aflatoxins not only on the outside, but also on the inside. Aflatoxins were first found in 1960 as toxic metabolites produced by A. flavus which killed numerous turkeys in England. They also were shown to have an extremely potent carcinogenicity toward mammals and found as contaminates in a wide variety of food commodities. Aflatoxin is now generally recognized not only as an extremely toxic contaminant in foods and feeds, but also as one of the certain risk factors for liver cancer in humans. 1 Thus, control and management of aflatoxins have become issues of concern.

▼全件表示

講演・口頭発表等

  • アジア人の食と健康と腸内フローラ 招待

    中山二郎

    第21回日本機能性食品医用学会総会  2023年12月 

     詳細を見る

    開催年月日: 2023年12月

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:琉球大学   国名:日本国  

  • ケール摂取が腸内細菌叢を介して慢性便秘症に及ぼす影響の解析

    松崎渓作,江崎充,木村勇祐,伊原栄吉,中山二郎

    第29回 日本生物工学会九州支部 大分大会  2023年12月 

     詳細を見る

    開催年月日: 2023年12月

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:九州大学馬出キャンパス   国名:日本国  

  • Remodeling and Crisis of Asian Gut Microbiome 招待 国際会議

    Jiro Nakayama

    20th Philippine Society for Probiotics and Functional Foods  2023年10月 

     詳細を見る

    開催年月日: 2023年10月

    記述言語:英語   会議種別:口頭発表(一般)  

    開催地:バイバイ   国名:フィリピン共和国  

  • 大豆ペプチドと環境エンリッチメントが マウスの腸内細菌叢に与える影響の解析

    荻山葵, 瀬々航紀, 黒木健悟,西川奈那, 濱野桃子, 古屋茂樹, 中山二郎

    学会創立100周年記念 日本農芸化学会中四国・西日本支部2023年度合同大会  2023年9月 

     詳細を見る

    開催年月日: 2023年9月 - 2025年9月

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:高知県立大学   国名:日本国  

  • アジア人の食と腸内フローラと健康 招待

    中山二郎

    第64回(令和5年度)果汁技術研究発表会  2023年9月 

     詳細を見る

    開催年月日: 2023年9月

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:ニッショーホール   国名:日本国  

  • Increase of non-communicable diseases in Asia is caused by change in diets and/or gut microbiota ? 招待 国際会議

    Jiro Nakayama

    The 13th Asian Conference on Lactic Acid Bacteria  2023年10月 

     詳細を見る

    開催年月日: 2023年8月

    記述言語:英語   会議種別:口頭発表(一般)  

    開催地:ウランバートル   国名:モンゴル国  

  • Crisis and Remodeling of Asian Gut Microbiome 招待 国際会議

    Jiro Nakayama

    The 8th International Conference of Indonesian Society for Lactic Acid Bacteria and Gut Microbiota  2023年7月 

     詳細を見る

    開催年月日: 2023年7月

    記述言語:英語   会議種別:口頭発表(一般)  

    開催地:バリ   国名:インドネシア共和国  

  • 炭素源の違いが合成腸内細菌叢に与える影響の解析

    大石紗菜、中山二郎

    第60回化学関連支部合同大会  2023年7月 

     詳細を見る

    開催年月日: 2023年7月

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:北九州国際会議場   国名:日本国  

  • 各種海藻多糖類が腸内細菌叢に及ぼす影響の比較解析

    太田匡俊、三島梨子、上野幹憲、中山二郎

    日本農芸化学会2023年度大会  2023年3月 

     詳細を見る

    開催年月日: 2023年3月

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:リモート(広島大学)   国名:日本国  

  • 直接作用型ウイルス剤(DAAs)による治療がC型肝炎患者の胆汁酸代謝に与える影響

    中川大輔、井上貴子、田中靖人、中山二郎

    日本農芸化学会2023年度大会  2023年3月 

     詳細を見る

    開催年月日: 2023年3月

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:リモート(広島大学)   国名:日本国  

  • 人間ドック受診者を対象とした腸内細菌叢と代謝疾患との関連性の解析

    田村史織、篠田あかり、井上貴子、渡辺久剛、田中靖人、中山二郎

    日本農芸化学会2023年度大会  2023年3月 

     詳細を見る

    開催年月日: 2023年3月

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:リモート(広島大学)   国名:日本国  

  • Interaction of diet and gut microbiome in Asians and its effect on their health 招待

    Jiro Nakayama

    22nd International Union of Nutritional Sciences  2022年11月 

     詳細を見る

    開催年月日: 2022年11月

    記述言語:英語   会議種別:口頭発表(一般)  

    開催地:東京国際フォーラム   国名:日本国  

  • Asian Microbiome Project to understand the current status of gut microbial community of Asians as an interface between foods and health 招待

    Jiro Nakayama

    The 12th Asian Conference on Lactic Acid Bacteria  2022年10月 

     詳細を見る

    開催年月日: 2022年10月

    記述言語:英語   会議種別:口頭発表(一般)  

    開催地:リモート(マレーシア・クチン)   国名:日本国  

  • Gut microbiome and metabolome of Asian population related to diet and health 招待

    Jiro Nakayama

    The 11th Asian Conference on Lactic Acid Bacteria  2021年11月 

     詳細を見る

    開催年月日: 2021年11月

    記述言語:英語   会議種別:口頭発表(一般)  

    国名:日本国  

  • Comparison of gut microbiota between diabetic and non-diabetic obese Mongolian 国際会議

    Akari SHINODA, Shirchin DEMBEREL, Riko MISHIMA, Dugersuren JAMYAN, Tsogtbaatar LKHAGVAJAV , Jiro NAKAYAMA

    The 11th Asian Conference on Lactic Acid Bacteria  2021年11月 

     詳細を見る

    開催年月日: 2021年11月

    記述言語:英語   会議種別:口頭発表(一般)  

    国名:日本国  

  • アジアの小学生児童の腸内細菌叢 招待

    中山二郎

    第25回腸 内細菌学会学術集  2021年6月 

     詳細を見る

    開催年月日: 2021年6月

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:東京   国名:日本国  

  • 各種ヒト胆汁酸による腸内芽胞形成細菌の発芽誘導と分離培養

    鬼塚咲良,田中優,中山二郎

    第25回腸 内細菌学会学術集会  2021年6月 

     詳細を見る

    開催年月日: 2021年6月

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:東京   国名:日本国  

  • アジアの小学生児童の腸内細菌叢 招待

    中山二郎

    第25回腸 内細菌学会学術集会  2021年6月 

     詳細を見る

    開催年月日: 2021年6月

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:東京   国名:日本国  

  • 腸内環境および免疫機能に及ぼすあまおう乳酸菌およびフコイダンの摂食効果

    篠田あかり,朴俊夏,中野勇人,柴崎哲哉, 宮﨑義之,中山二郎

    日本農芸化学会2021年度大会  2021年3月 

     詳細を見る

    開催年月日: 2021年3月

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:仙台   国名:日本国  

  • 日本人とインドネシア人の乳幼児の腸内メタゲノム比較解析

    清野峻彦,堀野美里,田中優,山田拓司,林哲也, 後藤恭宏,加藤聖子,實藤雅文,諸隈誠一,小川昌宣, Endang Rahayu,中山 二郎

    日本農芸化学会2021年度大会  2021年3月 

     詳細を見る

    開催年月日: 2021年3月

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:仙台   国名:日本国  

  • The succession of gut mycobiota in Japanese infants at first 3 years of life 国際会議

    Riko Mishima, Masafumi Sanefuji, Seiichi Morokuma, Masaru Tanaka, Masanobu Ogawa, Kiyoko Kato, Jiro Nakayama

    International Union of Microbiological Societies 2020  2020年10月 

     詳細を見る

    開催年月日: 2020年10月

    記述言語:英語   会議種別:口頭発表(一般)  

    開催地:Daejeon   国名:大韓民国  

  • アジア人の食と腸内フローラと生活習慣病 招待

    中山二郎

    Visionary 農芸化学100 シンポジウム 食・腸内細菌・健康研究領域 第3回シンポジウム「健康長寿社会に向けての腸内細菌科学の新展開  2021年9月 

     詳細を見る

    開催年月日: 2020年9月 - 2021年9月

    記述言語:日本語   会議種別:シンポジウム・ワークショップ パネル(公募)  

    開催地:リモート   国名:日本国  

  • 生後1年間における乳幼児の腸内真菌叢形成と出産・育児環境因子の関連性解析

    #三島 梨子、@實藤 雅文、@諸隈 誠一、#田中 優、百田 理恵、@園元 謙二、@小川 昌宣、@加藤 聖子、@中山 二郎

    日本農芸化学会2019年度大会  2019年3月 

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    開催年月日: 2019年3月 - 2018年3月

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:東京農業大学   国名:日本国  

    【背景】ヒト腸内では出生後まもなくして細菌、真菌、ウィルス等からなる腸内微生物叢 (腸内フローラ) の形成が始まる。乳幼児腸内フローラは出産方法、授乳方法、抗生物質といった出産・育児環境因子の影響を受けながら大きく変動していくのと同時に宿主の健康的な成長に様々な作用をもたらす。しかしながら、これらの研究は腸内細菌を対象にしたものがほとんどであり、腸内フローラの一員である真菌については未知の部分が多い。特に様々な環境因子が腸内真菌叢形成に与える影響についての報告例はほとんどない。そこで、我々は環境省の実施する「子どもの健康と環境に関する全国調査 (エコチル調査) 」に参加している乳幼児96名 (福岡市) を対象に生後1年間の腸内真菌叢に影響を与える環境因子を調査した。 【方法】本研究では、エコチル調査に参加している乳幼児96名を対象とし、生後1ヶ月時 (n = 83) および生後1年時 (n = 87) の糞便試料と質問票調査から出生・育児環境についての情報を使用した。糞便試料から抽出したDNAを鋳型に真菌リボソーマルRNA遺伝子ITS1領域を網羅的に増幅させ、Illmina社MiSeqによるペアエンドシーケンスを行った。得られた配列データからUparse/Usearchを用いてOTUを作成し、系統解析を行うことで各被験者の腸内真菌叢を決定した。この腸内真菌叢データと出生・育児環境調査の結果をもとに統計解析を行い環境因子が与える腸内真菌叢への影響を調査した。 【結果と考察】授乳期にあたる生後1ヶ月時の乳幼児腸内真菌叢では皮膚常在真菌であるMalassezia (45%) が最も多く、生後1年時に比べ生活環境中の真菌が多い傾向があった。離乳期にあたる生後1年時になるとヒト腸内常在真菌であるSaccharomyces (25%) が最優勢の真菌叢へと変化し、食事内容の変化が真菌叢構成に影響を与えることが推測された。また、生後1ヶ月では自然分娩児に比べ帝王切開児でEupenicillium (p = 0.02, Mann Whitney U-test) やAspergillus (p = 0.01) など生活環境中の真菌が多い傾向が見られた。生後1ヶ月での授乳方法については母乳栄養児にMalassezia (p = 0.002)、混合乳栄養児にSaccharomyces (p = 0.01) が有意に多い結果となったが、生後1年時ではこれらの有意差は喪失した。以上のことから、分娩様式および授乳法は生後1年に至るまでの真菌叢形成に影響することが示唆された。

  • 環状ペプチドクォルモンを介する腸内細菌と宿主のクロストークの可能性 招待

    中山二郎

    日本農芸化学会2019年度大会  2019年3月 

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    開催年月日: 2019年3月 - 2018年3月

    記述言語:日本語   会議種別:シンポジウム・ワークショップ パネル(公募)  

    開催地:東京農業大学   国名:日本国  

    生物はフェロモン、クオルモン、ホルモンのようなシグナル分子を用いて、個体間や細胞間の情報伝達を高度に成立させている。その背景には、シグナル分子とその受容体の共進化があり、無数に共存するシグナル分子から特定のシグナルのみを感知し、適材適所に応答する高度なシステムを進化させている。従来の生命科学においては、正規の受容体を通してのシグナル分子の作用に関する研究が盛んに行われ、個々の生命体でのシグナル分子とその伝達機構の多くが解明された。しかし地球上では、元来すべての生物が他の生命体と共生・寄生関係の中で生きており、種を超えたシグナル分子とシグナル伝達系のクロストークが共生関係に重要な働きをしている例も多いと考えられる。 我々は、これまでに同種細菌間のコミュニケーションシグナルとしてのクオルモンに着目して研究を進めてきた。中でもグラム陽性細菌に幅広く存在する環状チオラクトンあるいはラクトン構造を有するペプチドクオルモンは、ゲノムシーケンスデータベースを探索すると、その遺伝子保有菌種は500種、ペプチドシーケンスのバリエーションは1000種近くに上る。つまりグラム陽性菌では、これらの環状ペプチド分子を用いて種特異的なクオラムセンシングを自在に展開していると想像される。特に、これらのグラム陽性菌にはヒト腸内にて多岐にわたり常在するファーミキューテス門の細菌が多く、数百種の細菌が高密度にひしめき合うヒト腸管内では、この環状ペプチドクオルモンを用いたクオラムセンシングは重要な働きを有すると考えられる。しかし、これらの環状ペプチドクオルモンの多くは反応性が高く、構造安定性が悪く、他分子や宿主細胞と不可逆的に相互作用している可能性が疑われる。現在我々は、その可能性を追求して、宿主の免疫細胞との相互作用などに興味を持って研究を進めている。 我々はまた、クオラムセンシングの阻害という観点から天然物からの受容体アンタゴニストのスクリーニングを行ってきた。その結果、興味深いことに、タキキニン受容体のアンタゴニストとして知られるWS9326やエンドセリン受容体のアンタゴニストとして知られるCochinmicinが腸球菌のクオルモンであるGBAPのアンタゴニストとして機能することを見出した。そこで、GBAPがこれらのホルモンの受容体(GPCR)とクロストークするのではないかと考え、結合活性を調べた。その結果、非常に弱いながらもGBAPがGPCRに対して結合することが見出された。今回は、一つのクオルモンについてのみ調べた結果であるが、多様な腸内細菌のクオルモンと腸管で多岐にわたり発現しているGPCRとの間では様々なクロストークがあることが十分に予想され大変興味が持たれるところである。

  • Maturation of the infant gut microbiota community and bile acids metabolism over three years in healthy Japanese infants 国際会議

    #Masaru Tanaka, @M. Sanefuji, @S. Morokuma, #M. Yoden, R. Momoda, @K. Sonomoto, @K. Kato, @J. Nakayama

    7th Beneficial Microbes Conference  2018年11月 

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    開催年月日: 2018年11月

    記述言語:英語   会議種別:口頭発表(一般)  

    開催地:アムステルダム   国名:オランダ王国  

  • Signatures imprinted in gut microbiome of Asians: a comparative metagenomic study of children in East and South-East Asia 国際会議

    #Misato Horino, #M. Tanaka, #A. Uchikawa, R. Momoda, @K. Sonomoto, Y. Ogura, A. Toyoda, T. Yamada, K. Kurokawa, @T. Hayashi, @J.Nakayama

    7th Beneficial Microbes Conference  2018年11月 

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    開催年月日: 2018年11月

    記述言語:英語   会議種別:口頭発表(一般)  

    開催地:アムステルダム   国名:オランダ王国  

  • 日本人成人における腸内細菌叢と食事の関連性の解析

    内川彩夏,田中優, 余田美沙子,中山二郎, 池田温子, 河口礼佳, 十倉充範

    日本農芸化学会 関西・中四国・西日本支部2017年度合同大阪大会  2017年9月 

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    開催年月日: 2018年9月 - 2019年9月

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:大阪府立大学 中百舌鳥キャンパス   国名:日本国  

  • Urbanization of Thai diets and its impacts to children gut microbiota and metabolites, The International Conference on Beneficial Microbes 国際会議

    #J. Kisuse, #M. Tanaka, #P. Therdtatha, R. Momoda, O. La-ongkham, M. Nakphaichit, S. Nitisinpraser, S. Fukuda, @K. Sonomoto, @J. Nakayama

    ICOBM2018  2018年8月 

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    開催年月日: 2018年8月

    記述言語:英語   会議種別:口頭発表(一般)  

    開催地:マレーシア   国名:マレーシア  

  • アジア各国の腸内細菌叢の有する遺伝子機能に関する研究

    堀野美里、田中優、内川彩夏、百田理恵、園元謙二、小椋義俊、豊田敦、山田拓司、黒川顕、林哲也、中山二郎

    日本乳酸菌学会2018年度大会  2018年7月 

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    開催年月日: 2018年7月

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:東京農業大学   国名:日本国  

  • In vitro培養モデルを利用したヒト腸管における短鎖脂肪酸生産に関わる主要細菌群の選出

    冬野憂介、安達 桂香、田中優、園元 謙二、中山 二郎

    日本乳酸菌学会2018年度大会  2018年7月 

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    開催年月日: 2018年7月

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:東京農業大学   国名:日本国  

  • 腸内細菌叢研究から見た日本人の食生活と健康:Asian Microbiome Projectの研究成果から 招待

    中山二郎、Yuan-Kun Lee

    第72回日本栄養・食糧学会大会  2018年5月 

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    開催年月日: 2018年5月

    記述言語:日本語   会議種別:シンポジウム・ワークショップ パネル(公募)  

    開催地:岡山県立大学   国名:日本国  

  • 日本人健常人の腸内細菌叢における胆汁酸代謝に関する研

    余田美沙子、田中優、内川彩夏、百田理恵、池田温子、河口礼佳、園元謙二、十倉充範、中山二郎

    日本農芸化学会2018年度大会  2018年3月 

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    開催年月日: 2018年3月

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:名城大学   国名:日本国  

  • 日本人乳幼児の生後3年間の腸内フローラの形成・成熟化と胆汁酸代謝の関連性解析

    田中優、實藤雅文、諸隈誠一、余田美沙子、百田理恵、園元謙二、加藤聖子、中山二郎

    日本農芸化学会2018年度大会  2018年3月 

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    開催年月日: 2018年3月

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:名城大学   国名:日本国  

    【背景】乳児の腸内フローラは誕生直後に形成が始まり、2 年から 3 年かけて成熟していくと考えら れている。この腸内フローラ形成は、乳幼児期の健康だけではなく、後の宿主の生理にも影響してい ることが明らかになりつつある。しかし、この腸内フローラの形成・成熟化は細菌叢組成の解析が進 められているが、その機能、特に宿主との相互作用については未知の部分が多い。特に宿主の生理に 重要な働きをする胆汁酸は、腸内環境を大きく左右させるが、腸内フローラの成熟化とそれによる胆 汁酸代謝の変動、そしてそれが宿主の代謝恒常性に与える影響について興味が持たれる。そこで、本 研究では、乳幼児の生後 3 年間の腸内フローラの形成・成熟化と胆汁酸代謝の変動の関連性を明らか にすることを目的にした。 【方法】11 名の新生児とその母親を対象とし、母親からは出産前、新生児からは生後 1、3、6、12、 24、36 ヶ月時に糞便サンプルを回収した。腸内フローラは便サンプルより抽出した全細菌 DNA を鋳型 とし、16S rRNA 遺伝子 の V1-V2 領域の配列を解析した。便中の胆汁酸量は凍結乾燥した糞便サンプ ルからエタノールにより抽出し、LC-QMS により定量分析を行った。 【結果と考察】生後 3 年までの便中の胆汁酸組成は、抱合胆汁酸型、一次胆汁酸型、二次胆汁酸型、 ウルソデオキシコール酸(UDCA)型のいずれかの胆汁酸種が特徴的である4つの群にクラスター分けさ れた。生後初期は抱合胆汁酸型の乳児が多く、次いで一次胆汁酸型に変化し、離乳食開始とともに UDCA 型に変化し、その後、二次胆汁酸型へと移行する傾向が見られた。抱合胆汁酸型の乳児の腸内フロー ラは他と比較して Bifidobacterium がほとんど存在せず、 Bifidobacterium の定着を機に胆汁酸代謝 が開始されることが示唆された。一次胆汁酸型から UDCA 型への移行時には、 Ruminococcus gnavus の 優占化が見られた。UDCA は宿主に胆汁酸分泌を促進させ、脂質の消化・吸収を向上させることが知ら れており、また、 R. gnavus は栄養失調児の便を移植したモデルマウスの成長を回復させることが報 告されている。以上より、 R. gnavus の定着は乳幼児の成長に重要な働きを担っていることが予測さ れる。最終的(生後3年時)には、1名を除いたすべての乳幼児に二次胆汁酸型が観察された。二次 胆汁酸型では、多様な Firmicutes 門と Bacteroidetes 門を優占菌とする成人型腸内フローラが形成 されていた。以上、乳幼児期の細菌叢の成熟化が胆汁酸代謝と密接に関連している様子が伺えた。

  • 日本人健常成人の食と腸内細菌叢における短鎖脂肪酸の生産パターン

    内川彩夏、田中優、余田美沙子、百田理恵、園元謙二、中山二郎、池田温子、河口礼佳、十倉充範

    日本農芸化学会2018年度大会  2018年3月 

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    開催年月日: 2018年3月

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:名城大学   国名:日本国  

  • アジア人の腸内フローラとビフィズス菌 招待

    中山二郎

    腸内細菌と健康ービフィズス菌研究の新展開  2018年1月 

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    開催年月日: 2018年1月

    記述言語:日本語   会議種別:シンポジウム・ワークショップ パネル(公募)  

    開催地:品川インターシティーホール   国名:日本国  

  • アジアマイクロビオームプロジェクト:アジア人の食と健康のインターフェースとしての腸内フローラの理解に向けて 招待

    中山二郎

    第26回腸内フローラシンポジウム  2017年10月 

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    開催年月日: 2017年10月

    記述言語:日本語   会議種別:シンポジウム・ワークショップ パネル(公募)  

    開催地:ヤクルトホール   国名:日本国  

  • Metabolic-linked pH-drop quenches VirSR quorum sensing in Clostridium perfringens 国際会議

    Keika Adachi, Jiro Nakayama.

    6th ASM Cell-Cell communication in Bacteria  2017年10月 

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    開催年月日: 2017年10月

    記述言語:英語   会議種別:口頭発表(一般)  

    開催地:Athens  

  • Ecology of gut microbiota in Japanese children - possible link with pre- and probiotics research 招待 国際会議

    Jiro Nakayama

    6th Beneficial Microbes Conference  2017年10月 

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    開催年月日: 2017年10月

    記述言語:英語   会議種別:口頭発表(一般)  

    開催地:アムステルダム   国名:オランダ王国  

  • Clostridium perfringensの毒素生産制御に関わる内在性クオラムクエンチングの解析

    安達桂香、Ravindra Pal Singh、園元謙二、中山二郎

    第69回日本生物工学会年次大会  2017年9月 

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    開催年月日: 2017年9月

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:早稲田大学   国名:日本国  

  • Clostridium perfringensの代謝同調的な内在性クオラムクエンチング機構の実態解明

    安達桂香、Ravindra Pal Singh、大谷郁、園元謙二、中山二郎

    環境微生物系学会合同大会2017  2017年8月 

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    開催年月日: 2017年8月

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:東北大学川内北キャンパス   国名:日本国  

  • Changing dietary habit is changing Asians gut microbiota 招待 国際会議

    Jiro Nakayama

    15th International Congress of Bacteriology and Applied Microbiology  2017年7月 

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    開催年月日: 2017年7月

    記述言語:英語   会議種別:口頭発表(一般)  

    開催地:シンガポール   国名:シンガポール共和国  

    Objective: The impact of modernization in life style on the health of human is global issue at present. Particularly, westernization of diets links to certain diseases in developing area. Recent some studies have revealed that gut microbiota altered by the introduction of Western foods is involved in the increase of risk for certain diseases, e.g., colon cancer, obesity, or diabetes. Our international consortium project, Asian Microbiome Project, has also investigate the impact of globalization of diets on the gut microbial community in Asian population. Methods: Leyte island in Philippines is being modernized and children in the rural Baybay city and urban Ormoc city have different life style including daily diets. To know the impact of modernization of dietary habit on gut microbiota in preadolescent children, we compared fecal microbiota between Baybay (n=23) and Ormoc children (n=19) at the age of 7 to 9 years old and examined the correlation of bacterial composition with their dietary data. Results: Dietary survey indicated that Ormoc children consumed significantly higher level of fat than Baybay children, by eating modern high-fat foods. Fecal microbiota was analyzed by meta 16S rRNA analysis with the dataset of other five Asian countries. Their microbiota at family level were grouped into two enterotype-like clusters, each defined by the high abundance of Prevotellaceae (P-type) and Bacteroidaceae (BB-type), respectively. Baybay and Ormoc children mainly harbored P-type and BB-type, respectively. Redundancy analysis with their dietary nutrients showed that P-type and BB-type favors carbohydrate and fat, respectively. Fat intake level correlated positively with Firmicutes-to-Bacteroidetes (F/B) ratio and negatively with relative abundance of genus Prevotella. Overweight and obese children who were living in Ormoc and took higher level of fat, harbored the microbiota with higher F/B ratio and lower abundance of Prevotella. The altered gut microbiota may be a sign of modern diet-induced obesity among children in developing areas. Conclusion: Oriental and Western dietary cultures are now mixing in Asia and remodeling gut microbiota of Asians. To ensure our healthiness in feature, it should be important to understand the link between diets, gut microbiota, and health, in more depth.

  • 健常成人における胆汁酸プロファイルと腸内細菌叢の関連性の解析

    余田美沙子、田中優、内川彩夏、本田倫子、百田理恵、池田温子、河口礼佳、園元謙二、十倉充範、中山二郎

    日本乳酸菌学会2017年度大会  2017年7月 

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    開催年月日: 2017年7月

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:玄海ロイヤルホテル   国名:日本国  

  • Clostridium perfringensの内在性クオラムクエンチング―代謝同調的pH低下によるクオラムセンシング機構の抑制―

    安達桂香、神川美樹、Ravindra Pal Singh、大谷郁、園元謙二、中山二郎

    日本農芸化学会  2017年3月 

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    開催年月日: 2017年3月 - 2016年3月

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:京都女子大学   国名:日本国  

  • Clostridiales目細菌ゲノムにコードされるagr様クオラムセンシング系自己誘導ペプチドのLC-MS/MS検出系の確率

    中山二郎、神川美樹、安達桂香、大久保謙一、大谷郁、岡健太郎、園元謙二

    日本農芸化学会  2017年3月 

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    開催年月日: 2017年3月 - 2016年3月

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:京都女子大学   国名:日本国  

  • 腸内細菌叢研究の概論 招待

    中山二郎

    日本アレルギー学会イブニングシンポジウム  2016年12月 

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    開催年月日: 2016年12月

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:パシフィコ横浜   国名:日本国  

  • ヒト腸内細菌叢のダイナミズムとダイバーシティー 招待

    中山二郎

    日本乳酸菌学会秋期セミナー  2016年12月 

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    開催年月日: 2016年12月

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:明治大学生田キャンパス   国名:日本国  

  • Changing dietary habit is changing Asian gut microbiota 招待 国際会議

    Jiro Nakayama

    5th Beneficial Microbes Conference  2016年10月 

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    開催年月日: 2016年10月

    記述言語:英語   会議種別:口頭発表(一般)  

    開催地:Amsterdam   国名:オランダ王国  

  • アジア人の腸内細菌叢 招待

    中山二郎

    Visonary農芸化学100シンポジウム  2016年10月 

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    開催年月日: 2016年10月

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:京都大学益川ホール   国名:日本国  

  • アジア人の食と腸内細菌叢の関連性 招待

    中山二郎、本田倫子、Kisuse Juma、百田理恵、田中優、園元謙二、Lee Yuan-Kun

    日本生物工学会  2016年9月 

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    開催年月日: 2016年9月

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:富山国際会議場   国名:日本国  

  • Clostridium butyricum MIYAIRI 588が産生するオートインデューサー様ペプチドの同定

    神川美樹、庄島あかね、大久保謙一、松藤貴久、Ravindra Pal Siingh, 大谷郁、岡健太郎、高橋志達、園元謙二、中山二郎

    第52回化学関連支部合同九州大会  2015年6月 

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    開催年月日: 2016年6月

    記述言語:日本語  

    開催地:北九州   国名:日本国  

  • 新生児の生後1年間の腸内細菌叢変化と食物アレルギー発症の関連性の解析

    田中優、東佳那子、山本麻寿紗、本田倫子、百田理恵、清原千賀子、園元謙二、鷲尾昌一、中山二郎

    第20回腸内細菌学会  2016年6月 

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    開催年月日: 2016年6月

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:東京大学   国名:日本国  

  • Development of quorum quenching peptides targeting VirR/VirS system of Clostridium perfringens 国際会議

    Ravindra Pal Singh, Kenich Okubo, Kenji Sonomoto, Jiro Nakayama

    6th Congress of European Microbiologists  2015年6月 

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    開催年月日: 2016年6月

    記述言語:英語  

    開催地:Maastricht   国名:オランダ王国  

  • Clostridium perfringensの毒素産生を制御するクオラムクエンチングの機構の解明

    安達桂香、大谷郁、河野通生、ラビンドラパルシン、園元謙二、中山二郎

    日本農芸化学会2016年度大会  2016年3月 

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    開催年月日: 2016年3月

    開催地:札幌   国名:日本国  

  • 食物アレルギー発症児と非発症児の乳幼児期における腸内細菌叢の比較解析

    田中優、東佳那子、山本麻寿紗、本田倫子、百田理恵、清原千賀子、園元謙二、鷲尾昌一、中山二郎

    日本農芸化学会2016年度大会  2016年3月 

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    開催年月日: 2016年3月

    記述言語:日本語  

    開催地:札幌   国名:日本国  

  • フィリピンの子どもの腸内細菌叢:食との関連性

    山本麻寿紗、本田倫子、田中優、百田理恵、Ladie Palermo, Jullie Tan, Yuan Kun Lee, 園元謙二、中山二郎

    第22回日本生物工学会九州支部宮崎大会  2015年12月 

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    開催年月日: 2015年12月

    記述言語:日本語  

    開催地:宮崎   国名:日本国  

  • Clostridium perfringensの毒素産生を制御するクオラムクエンチングに関する研究

    安達桂香、Ravindra Pal Singh,大谷郁、河野通生、園元謙二、中山二郎

    第22回日本生物工学会九州支部宮崎大会  2015年12月 

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    開催年月日: 2015年12月

    記述言語:日本語  

    開催地:宮崎   国名:日本国  

  • Age-related changes and their food consumption affecting gut microbiota of healthy Thai subjects 招待 国際会議

    Sunee Nitisinprasert, Orawan La-ongkam, Supatjaree Ruengsomwong, Massalin Nakphaichit, Bhusita Wanissorn, Jiro Nakayama

    第67回日本生物工学会  2015年10月 

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    開催年月日: 2015年10月

    記述言語:英語   会議種別:口頭発表(一般)  

    開催地:鹿児島   国名:日本国  

  • 新生児期における腸内細菌叢に影響を与える要因の網羅的解析

    本田倫子、實藤雅文、諸隈誠一、山本麻寿紗、田中優、百田理恵、高木愛子、佐藤匡夫、月森清巳、加藤聖子、原寿郎、中山二郎

    第67回日本生物工学会  2015年10月 

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    開催年月日: 2015年10月

    記述言語:日本語  

    開催地:鹿児島   国名:日本国  

  • フィリピン都市部と農村部に住む児童の腸内細菌叢の比較

    山本麻寿紗、本田倫子、田中優、百田理恵、Ladie Palermo, Jullie Tan, Yuan Kun Lee, 園元謙二、中山二郎

    2015年度日本農芸化学会中四国・西日本支部合同大会  2015年9月 

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    開催年月日: 2015年9月

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:愛媛   国名:日本国  

  • Digging into our gut microbial community by NGS 招待 国際会議

    Jiro Nakayama

    2nd Asian Fermented Foods: Probiotic: the potential ingredients for health products  2015年8月 

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    開催年月日: 2015年8月

    記述言語:英語   会議種別:口頭発表(一般)  

    開催地:Bangkok   国名:タイ王国  

  • What is microbiota, microbiome and their analysis ? 招待 国際会議

    Jiro Nakayama

    The 8th Asian Conference on Lactic Acid Bacteria  2015年7月 

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    開催年月日: 2015年7月

    記述言語:英語   会議種別:口頭発表(一般)  

    開催地:Bangkok   国名:タイ王国  

  • アジア人の食と腸内細菌と健康に関するマルチコホート研究 招待

    中山二郎

    第19回腸内細菌学会  2015年6月 

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    開催年月日: 2015年6月

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:東京   国名:日本国  

  • Asian Microbiome Project:アジア人の食と腸内フローラと健康に関する調査研究 招待

    中山二郎、渡辺幸一、Yuan-Kun Lee

    日本農芸化学会2014年度大会  2014年3月 

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    開催年月日: 2014年3月

    記述言語:日本語   会議種別:シンポジウム・ワークショップ パネル(公募)  

    開催地:東京   国名:日本国  

  • 放線菌の生産する環状デプシペプチドによるクオラムセンシング阻害活性

    庄島あかね, 園元謙二, 中山二郎, Said E Desouky, 大久保謙一

    日本農芸化学会2014年度大会  2014年3月 

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    開催年月日: 2014年3月

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:東京   国名:日本国  

  • アジア人の腸内フローラに見られる2つのエンテロタイプと地域特異性

    東佳那子、Jiahui Jiang, 渡辺幸一、Shirchin Demberel, Sunee Nitisinprasert, Yongha Park, Endang S, Rahayum, Fa-Zheng Ren, Ying-Chieh Tsai, 園元謙二、Yuan-Kun Lee, 中山二郎

    日本農芸化学会2014年度大会  2014年3月 

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    開催年月日: 2014年3月

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:東京   国名:日本国  

  • 微生物二次代謝産物を対象としたグラム陽性病原細菌のクオラムセンシング阻害物質の探索

    松藤貴久, 園元謙二, 中山二郎, Said E Desouky

    第20回生物工学会九州支部佐賀大会  2013年12月 

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    開催年月日: 2013年12月

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:佐賀   国名:日本国  

  • Diversity in gut bacterial composition and their 16S rRNA gene sequences among Asian children

    Jihaui Jiang, Kenji Sonomoto, Jiro Nakayama

    第37回講演会(日本農芸化学会関西・中四国・西日本支部、日本ビタミン学会近畿・中四国・九州沖縄合同大会  2013年9月 

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    開催年月日: 2013年9月

    記述言語:英語   会議種別:口頭発表(一般)  

    開催地:東京   国名:日本国  

  • グラム陽性日和見感染菌のクオラムセンシング阻害剤のスクリーニングと構造解析

    庄島あかね, 園元謙二, 中山二郎, 松藤貴久

    第50回科学関連合同支部九州大会  2013年7月 

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    開催年月日: 2013年7月 - 2013年9月

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:北九州   国名:日本国  

  • アジア5ヶ国児童の腸内細菌叢解析

    Jiro Nakayama, Jiahui Jiang, Koichi Watanabe, Kazunori Matsuda, Takashi Kurakawa, Hirokazu Tsuji, Fa-Zheng Ren, Sunee Nitisinprasert, Endang S, Rahayum Chii-Cherng Liao, Ying-Chieh Tsai, Yuan-Kun Lee

    第17回腸内細菌学会  2013年6月 

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    開催年月日: 2013年6月

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:東京   国名:日本国  

  • Phase I study of Asian Microbiome Project: Discovery of intestinal microbiota in Asian youngsters profiled bu 16S rRNA gene pyrosequencing

    Jiang J, Nakayama J, Watanabe K, Sakamoto N, Matsuda K, Kurakawa T, Tsuji H, Ren FZ, Nitisinprasert S, Rahayu ES, Liao CC, Tsai YC, Lee YK

    日本農芸化学会2013年度大会  2013年3月 

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    開催年月日: 2013年3月

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:仙台   国名:日本国  

  • 腸内細菌と健康:ゆりかごから墓場まで 招待

    中山二郎

    日本微生物連盟フォーラム「驚きの微生物たち」  2013年1月 

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    開催年月日: 2013年1月

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:東京   国名:日本国  

  • 腸球菌のクオラムセンシング阻害剤ZBzl-YAA5911の創製とその抗感染症剤としての効果検証

    中山二郎,横畑綾治,松藤貴久,鈴木崇,永田宏次,園元謙二

    第35回日本分子生物学会  2012年12月 

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    開催年月日: 2012年12月

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:福岡   国名:日本国  

  • 長期熟成糠床に生息する2つの乳酸桿菌のゲノム・生理特性解析

    加唐圭太、阪本直茂、森一樹、田代康介、園元謙二、中山二郎

    第19回九州支部大分大会  2012年6月 

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    開催年月日: 2012年6月

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:別府   国名:日本国  

  • 454 Pyrosequencing study on the basal microbiota of healthy Asian youngsters 国際会議

    Jiang J, Nakayama J, Watanabe K, Sakamoto N, Matsuda K, Kurakawa T, Tsuji H, Ren FZ, Nitisinprasert S, Rahayu ES, Liao CC, Tsai YC, Lee YK

    3rd Asain Symposium on Lactic Acid Bacteria  2012年5月 

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    開催年月日: 2012年5月

    記述言語:英語   会議種別:口頭発表(一般)  

    開催地:Wuxi   国名:中華人民共和国  

  • AN OVERVIEW OF LACTOBACILLUS REUTERI KUB-AC5 AS AN ANTIMICROBIAL SUBSTANCE PRODUCER AND PROBIOTIC 招待 国際会議

    Sunee Nitisinprasert, Massalin Nakphaichit, Nuntaporn Pungsungvorn, Jiraporn Tangthong, Arpa Suwatwattanakul, Jiro Nakayama, Kenji Sonomoto

    International Union of Microbiological Societies 2011 Congress  2011年9月 

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    記述言語:英語   会議種別:口頭発表(一般)  

    開催地:札幌  

  • ASIAN MICROBIOME PROJECT 招待 国際会議

    Yuan Kun Lee, Jiro Nakayama, Fazheng Ren, Sunee Nitisinprasert, Endang Rahayu, Raha Abdul Rahim, Chii Cherng Liao, Koichi Watanabe, Ying Chieh Tsai

    International Union of Microbiological Societies 2011 Congress  2011年9月 

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    記述言語:英語   会議種別:口頭発表(一般)  

    開催地:札幌  

  • COMMUNITY STRUCTURE AND IMMUNE FUNCTION OF DEVELOPMENTAL GI TRACT MICROBIOTA IN INFANTS 招待 国際会議

    Jiro Nakayama

    International Union of Microbiological Societies 2011 Congress  2011年9月 

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    記述言語:英語   会議種別:口頭発表(一般)  

    開催地:札幌  

  • COMMUNITY STRUCTURE AND IMMUNE FUNCTION OF DEVELOPMENTAL GI TRACT MICROBIOTA IN INFANTS 招待 国際会議

    Jiro Nakayama

    International Union of Microbiological Societies 2011 Congress  2011年9月 

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    記述言語:英語   会議種別:口頭発表(一般)  

    開催地:札幌  

  • Discovery and Development of Inhibitors Targeting Cyclic Peptide-mediated Quorum Sensing in Gram-positive Pathogens 招待 国際会議

    Jiro Nakayama

    4th ASM conference on cell-cell communication in bacteria  2011年11月 

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    記述言語:英語   会議種別:口頭発表(一般)  

    開催地:マイアミ   国名:アメリカ合衆国  

  • Asian Microbiome Project: A pilot study on the basal microbiota profile of healthy Asian youngsters 国際会議

    Nakayama J, Watanabe K, Sakamoto N, Jiang J, Matsuda K, Kurakawa T, Tsuji H, Ren FZ, Nitisinprasert S, Rahayu ES, Liao CC, Tsai YC, Lee YK

    International Human Microbiome Conference  2012年3月 

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    記述言語:英語  

    開催地:パリ   国名:フランス共和国  

  • 日本人成人腸内細菌叢の7クラスターの短鎖脂肪酸・胆汁酸代謝機能性と食生活習慣との関係性

    田中優、余田美沙子、内川彩夏、百田理恵、園元謙二、池田 温子、河口礼佳、十倉充範、亀山恵司、中山二郎

    日本乳酸菌学会  2019年7月 

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    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:岐阜   国名:日本国  

  • Features and signatures in Asian microbiome: Crisis or adaptation ? 招待 国際会議

    Jiro Nakayama

    The 10th Asian Conference on Lactic Acid Bacteria  2019年8月 

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    記述言語:英語   会議種別:口頭発表(一般)  

    開催地:ジョグジャカルタ   国名:インドネシア共和国  

  • アジア人の食習慣と腸内細菌叢と健康に関する研究 招待

    中山二郎

    第71回日本生物工学会大会  2019年9月 

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    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:岡山   国名:日本国  

  • 出生・育児環境が乳幼児期の腸内真菌叢形成に及ぼす影響

    三島梨子,實藤雅文,諸隈誠一,田中優,園元謙二,小川昌宣,加藤聖子, 中山二郎

    日本農芸化学会2019年度西日本・中四国支部合同沖縄大会  2019年11月 

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    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:沖縄   国名:日本国  

  • Association of diets with intestinal microbiome in Asians 招待 国際会議

    Jiro Nakayama

    The 7th Iternational Conference on Food Factors  2019年12月 

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    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:神戸   国名:日本国  

  • 腸内細菌と口腔内細菌の胆汁酸耐性

    船津結妃,田中 優,鬼塚咲良,中山二郎

    第26回日本生物工学会九州支部長崎大会  2019年12月 

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    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:長崎   国名:日本国  

  • 胆汁酸の発芽誘導活性を利用した腸内芽胞形成細菌の分離と同定

    田中 優,鬼塚 咲良,船津 結妃,中山 二郎

    第93回日本細菌学会総会  2020年2月 

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    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:名古屋   国名:日本国  

  • Difference of Gut Microbial Metabolism among Indonesian Obese, Type 2 Diabetic, and Healthy Groups

    Yayi Song, Phatthanaphong Therdtatha, Masaru Tanaka, Mariyatun Mariyatun, Miisaroh Almunifah, Nancy Eka Putri Manurung, Siska Indriarshi, Yuan-kun, Lee, Endang Sutriswati Rahayu, Jiro Nakayama

    日本農芸化学会2020年度大会  2020年3月 

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    記述言語:英語   会議種別:口頭発表(一般)  

    開催地:福岡   国名:日本国  

  • Difference of Gut Microbial Community in Indonesian Obese, Type 2 Diabetic, and Healthy Groups

    Phatthanaphong Therdtatha, Masaru Tanaka, Mariyatun Mariyatun, Miisaroh Almunifah, Nancy Eka Putri Manurung, Siska Indriarshi, Yuan-kun, Lee, Endang Sutriswati Rahayu, Jiro Nakayama

    日本農芸化学会2020年度大会  2020年3月 

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    記述言語:英語   会議種別:口頭発表(一般)  

    開催地:福岡   国名:日本国  

  • モンゴル人の腸内フローラと生活習慣病との関係

    篠田あかり、デンベレルシルチン、三島梨子、田中優、堀野美里、ジャムヤンドゴースレン, ラハクバジャウツォクトバートル、中山二郎

    日本農芸化学会2020年度大会  2020年3月 

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    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:福岡   国名:日本国  

  • ヒト腸内芽胞形成細菌の各種胆汁酸による発芽誘導と分離培養

    鬼塚咲良,田中 優,中山二郎

    日本農芸化学会2020年度大会  2020年3月 

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    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:福岡   国名:日本国  

  • 腸内フローラにおける胆汁酸代謝と健康と疾病について俯瞰する 招待

    中山二郎、田中優、船津結妃

    日本農芸化学会2020年度大会  2020年3月 

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    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:福岡   国名:日本国  

  • 乳幼児における腸内細菌叢形成とアレルギー発症に関する研究

    田中 重光、中山 二郎、SONGJINDA, Prapa、立山 敦、小林 貴子、清原 千香子、園元 謙二

    日本農芸化学会  2008年3月 

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    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:名古屋・名城大学   国名:日本国  

  • Peptide-mediated cell-cell communication in Gram-positive bacteria 招待 国際会議

    Nakayama, J.

    The XVth international symposium on gnotobiology  2005年7月 

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    記述言語:英語   会議種別:シンポジウム・ワークショップ パネル(公募)  

  • 分子疫学研究による乳幼児腸内細菌叢とアレルギーの関連性の解析

    田中 重光、SONGJINDA, Prapa、立山 敦、坪内 美樹、清原 千香子、白川 太郎、中山 二郎、園元 謙二

    日本農芸化学会  2006年3月 

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    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:京都   国名:日本国  

  • 腸球菌のfsr系を制御する環状ペプチドGBAPの構造活性相関

    西口 賢三、中山 二郎、園元 謙二

    日本農芸化学会  2006年3月 

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    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:京都   国名:日本国  

  • リステリア属細菌のagr様遺伝子群にコードされる環状ペプチドクォルモンの構造決定

    朱雀 奈津子、西口 賢三、善藤 威史、中山 二郎、園元 謙二

    日本農芸化学会  2008年3月 

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    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:名古屋・名城大学   国名:日本国  

  • 腸球菌のクォーラムセンシングを制御する環状ペプチドクォルモン(GBAP)の2つの芳香族アミノ酸は受容体への結合に関与する

    西口 賢三、中山 二郎1、永田 宏次、田之倉 優、園元 謙二

    日本農芸化学会  2008年3月 

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    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:名古屋・名城大学   国名:日本国  

  • 糸状菌二次代謝産物を対象とした腸球菌のクォーラムセンシング阻害物質の探索

    上村 結美、中山 二郎、五十嵐 康弘、西口 賢三、園元 謙二

    日本農芸化学会  2008年3月 

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    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:名古屋・名城大学   国名:日本国  

  • 複合型糖鎖を資化するBarnesiella属腸内細菌の解析

    土井佳奈子二郎,樋口裕次郎,竹川 薫,中山二郎

    第29回 日本生物工学会九州支部 大分大会  2023年12月 

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    開催年月日: 2023年12月

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:九州大学馬出キャンパス   国名:日本国  

  • Analysis of relationship between intestinal microbiota and metabolic diseases in health checkup patients 国際会議

    Shiori Tamura, Akari Shinoda, Takako Inoue, Hisayoshi Watanabe, Yasuhito Tanaka, Jiro Nakayama

    20th Philippine Society for Probiotics and Functional Foods  2023年10月 

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    開催年月日: 2023年10月

    記述言語:英語   会議種別:口頭発表(一般)  

    開催地:バイバイ   国名:フィリピン共和国  

  • モンゴル遊牧民の腸内細菌叢と発酵馬乳”アイラグ”の細菌叢に関する研究

    永島 歩武、篠田 あかり、Lhagvajav Tsogtaatar、Shirchin Demberel、中山 二郎

    日本乳酸菌学会2023年度大会  2023年7月 

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    開催年月日: 2023年7月

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:関西大学   国名:日本国  

  • 各種腸内細菌における胆汁酸脱抱合活性の比較解析

    寺下優理香、中山二郎

    第60回化学関連支部合同大会  2023年7月 

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    開催年月日: 2023年7月

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:北九州国際会議場   国名:日本国  

  • 各種腸内細菌におけるドーパミン生産の評価

    田村史織, 松崎渓作,中山二郎

    第59回化学関連支部合同大会  2022年7月 

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    開催年月日: 2022年7月

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:北九州国際会議場   国名:日本国  

  • in vitro 実験系を用いたフコイダンのヒト腸内細菌叢に 対する効果の解析

    大野波月,中野勇人,柴崎哲哉,里山俊哉, 宮﨑義之,中山二郎

    日本農芸化学会2022年度(令和4年度)大会  2022年3月 

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    開催年月日: 2022年3月

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:リモート   国名:日本国  

  • バクテロイデス属細菌の胆汁酸脱抱合活性の比較解析

    今西 陸,パタナポン ターダッタ,中山 二郎

    日本農芸化学会2022年度(令和4年度)大会  2022年3月 

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    開催年月日: 2022年3月

    記述言語:日本語   会議種別:口頭発表(一般)  

    国名:日本国  

  • ヒトマイクロバイオーム解析のための推奨分析手法開発 および室間共同試験による検証

    成田興司,Dieter M Tourlousse,三浦隆匡,大山良文, 島村麻美子,古川雅崇,坂本光央,大熊盛也, 清水律子,齋藤さかえ,後藤まき,中山二郎, 三島梨子,國澤純,細見晃司,亀山恵司,久田貴義, 笠原堅,川﨑浩子,関口勇地,寺内淳

    日本農芸化学会2022年度(令和4年度)大会  2022年3月 

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    開催年月日: 2022年2月 - 2022年3月

    記述言語:日本語   会議種別:口頭発表(一般)  

    国名:日本国  

  • バクテロイデス属細菌の胆汁酸脱抱合活性の解析

    今西陸、Phatthanaphong Therdtatha、中山二郎

    第27回 日本生物工学会九州支部 大分大  2021年12月 

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    開催年月日: 2021年12月

    記述言語:日本語   会議種別:口頭発表(一般)  

    国名:日本国  

  • 糖セラミドが腸内細菌 Blautia coccoides に与える作用の in vitro 解析

    田邉くるみ、大塚輝、岩下そのこ 、戴凰凰 、中山二郎、北垣浩志

    第27回 日本生物工学会九州支部 大分大会  2021年12月 

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    開催年月日: 2021年11月 - 2021年12月

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:リモート   国名:日本国  

  • アジアの小学生児童の腸内細菌叢 招待

    中山二郎

    第58回日本臨床生理学会総会  2021年11月 

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    開催年月日: 2021年11月 - 2020年11月

    記述言語:日本語   会議種別:シンポジウム・ワークショップ パネル(公募)  

    国名:日本国  

  • 腸内細菌叢の構造と機能の多様性 招待

    中山二郎

    第11回社会神経科学研究会  2021年11月 

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    開催年月日: 2021年10月 - 2021年11月

    記述言語:日本語   会議種別:口頭発表(一般)  

    国名:日本国  

  • 糖セラミドの腸内細菌への影響のin vitro解析

    大塚 輝, 戴 凰凰, 永留 真優, 満生 萌水, 田中 猛豪, 中山 二郎, 北垣 浩志

    日本生物工学会大会講演要旨集  2021年10月  (公社)日本生物工学会

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    開催年月日: 2021年10月

    記述言語:日本語  

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  • Gut microbiome and metabolome of Asians associated with diets and health 招待 国際会議

    Jiro Nakayama

    The 6th International Conference of Indonesian Society for Lactic Acid Bacteria and Gut Microbiota  2021年8月 

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    開催年月日: 2021年8月

    記述言語:英語   会議種別:口頭発表(招待・特別)  

    国名:インドネシア共和国  

  • アジア人の食習慣と腸内フローラと健康 招待

    中山二郎

    第31回西日本食品産業創造展  2021年5月 

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    開催年月日: 2021年5月

    記述言語:日本語   会議種別:口頭発表(一般)  

    国名:日本国  

  • Clostridium perfringensのagrBDノックアウト変異株におけるペリクル状買フィルム形成の分子機構の解明

    伊藤瞭,重岡愛莉,本田香代,安達桂香,大谷郁, 中山二郎

    日本農芸化学会2021年度大会  2021年3月 

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    開催年月日: 2021年3月

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:仙台   国名:日本国  

  • 糖付加型バクテリオシン,エンテロシンF4-9の構造と生合成機構の解析

    MAKY Mohamed Abdelfattah, MAKY Mohamed Abdelfattah, 石橋直樹, PEREZ Rodney Honrada, PEREZ Rodney Honrada, DOUD Jehan Ragab, 園元謙二, 中山二郎, 善藤威史

    日本乳酸菌学会誌  2021年 

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    開催年月日: 2021年

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  • 自然発酵パン種サワードウにおける乳酸菌と酵母の共生メカニズムの検証と考察

    大城麦人, 田中優, 百田理恵, 善藤威史, 中山二郎

    日本乳酸菌学会誌  2021年 

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    開催年月日: 2021年

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  • 胆汁酸とアミノ酸による胞子発芽誘導能活性を利用した腸内細菌の培養

    鬼塚咲良, 三島梨子, 田中優, 中山二郎

    腸内細菌学雑誌  2021年 

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    開催年月日: 2021年

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  • フィリピン人の肥満と腸内藍菌叢とその代謝物の関連性

    渡邉麻衣,SIANOYA AC,ELEGADO FB,DALMACIO LM,中山二郎

    2020年度フードファクター学会・日本農芸化学会西日本支部大会  2020年11月 

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    開催年月日: 2020年11月

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:宮崎   国名:日本国  

  • Clostridium属細菌におけるagr制御系の機能解析

    重岡愛莉,Basit Yousuf,東聖也,岡健太郎,高橋志達,中山二郎

    2020年度フードファクター学会・日本農芸化学会西日本支部大会  2020年11月 

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    開催年月日: 2020年11月

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:宮崎   国名:日本国  

  • Clostridium perfringensにおける自己誘導性ペプチドを介した宿主免疫回避能の解析

    本田香代、安達桂香、永尾潤一、田中芳彦、大谷郁、中山二郎

    2020年度フードファクター学会・日本農芸化学会西日本支部大会  2020年11月 

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    開催年月日: 2020年11月

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:宮崎   国名:日本国  

  • 日本の発酵食品の基盤、麹グリコシルセラミドは脂質代謝を改善する

    北垣 浩志, 浜島 弘史, 光武 進, 田中 優, 宮川 幸, 西向 めぐみ, 中村 強, 柳田 晃良, 中山 二郎, 永尾 晃治

    日本生物工学会大会講演要旨集  2019年8月  (公社)日本生物工学会

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    開催年月日: 2019年8月

    記述言語:日本語  

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  • 食品成分が腸内環境に及ぼす影響 アジア人の食習慣と腸内細菌叢と健康に関する研究

    中山 二郎

    日本生物工学会大会講演要旨集  2019年8月  (公社)日本生物工学会

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    開催年月日: 2019年8月

    記述言語:日本語  

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  • 日本の発酵食品に関する新展開 麹菌由来グリコシルセラミドの腸内環境改善効果

    小濱 可奈絵, 田中 優, 浜島 弘史, 中村 強, 柳田 晃良, 西向 めぐみ, 光武 進, 中山 二郎, 永尾 晃治, 北垣 浩志

    日本栄養・食糧学会大会講演要旨集  2019年4月  (公社)日本栄養・食糧学会

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    開催年月日: 2019年4月

    記述言語:日本語  

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  • 日本人の腸内フローラの特徴と疾病との関係 招待

    中山二郎

    第79回 関西耳鼻咽喉科アレルギー研究会  2018年12月 

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    開催年月日: 2018年12月

    記述言語:日本語   会議種別:口頭発表(招待・特別)  

    開催地:KPガーデンシティPREMIUM心斎橋バンケット   国名:日本国  

  • Comparative study on the link between diet and gut microbiota in rural and urban children in Thailand 国際会議

    Juma Kisuse, Masaru Tanaka, Ayaka Uchikawa, Misako Yoden, Rie Momoda, Kenji Sonomoto, Jiro Nakayama

    International Symposium on Agricultural, Food, Environmental and Life Sciences in Asia  2017年11月 

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    開催年月日: 2018年11月

    記述言語:英語   会議種別:口頭発表(一般)  

    開催地:九州大学医学部百年講堂   国名:日本国  

  • 腸内フローラ研究からみた日本人とアジア人の健康 招待

    中山二郎

    日本食生活学会第57回大会  2018年10月 

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    開催年月日: 2018年10月

    記述言語:日本語   会議種別:シンポジウム・ワークショップ パネル(公募)  

    開催地:中村学園大学   国名:日本国  

  • Introduction of Asian Microbiome Project: Comparative gut microbiome study within and across countries 招待 国際会議

    Jiro Nakayama

    Bio Japan 2018  2018年10月 

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    開催年月日: 2018年10月

    記述言語:英語   会議種別:シンポジウム・ワークショップ パネル(公募)  

    開催地:パシフィコ横浜国立横浜国際会議場   国名:日本国  

  • アジアの食と腸内フローラ 招待

    中山二郎

    第45回日本小児栄養消化器肝臓学会  2018年10月 

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    開催年月日: 2018年10月

    記述言語:日本語   会議種別:口頭発表(招待・特別)  

    開催地:大宮ソニックシティー   国名:日本国  

  • アジア人の食生活と腸内菌叢の解明から見えてきた今後の食品開発 招待

    中山二郎

    食品開発展2018  2018年10月 

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    開催年月日: 2018年10月

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:東京ビックサイト   国名:日本国  

  • グリコシルセラミドの腸内細菌への影響解析

    北島 悠花, 佐藤 友哉, 宮川 幸, 阪本 真由子, 許 志豪, 中山 二郎, 北垣 浩志

    日本生物工学会大会講演要旨集  2018年8月  (公社)日本生物工学会

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    開催年月日: 2018年8月

    記述言語:日本語  

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  • サワードウの継代過程における化学成分変化と微生物叢変化の関連性

    大城 麦人, 百田 理恵, 田中 優, 中山 二郎

    日本生物工学会大会講演要旨集  2018年8月  (公社)日本生物工学会

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    開催年月日: 2018年8月

    記述言語:日本語  

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  • 腸内フローラ形成と宿主免疫系構築との関連性 招待

    中山二郎

    第56回埼玉県小児感染免疫懇話会  2018年7月 

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    開催年月日: 2018年7月

    記述言語:日本語   会議種別:口頭発表(招待・特別)  

    開催地:大宮ソニックシティ   国名:日本国  

  • 食と健康のインターフェースとしての腸内フローラの機能

    #三島梨子、@實藤雅文、@諸隈誠一、#田中優、@園元謙二、@加藤聖子、@中山二郎

    第55回化学関連支部合同九州大会  2018年7月 

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    開催年月日: 2018年7月

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:北九州国際会議場   国名:日本国  

  • 食と健康のインターフェースとしての腸内フローラの機能 招待

    中山二郎

    第55回化学関連支部合同九州大会  2018年7月 

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    開催年月日: 2018年7月

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:北九州国際会議場   国名:日本国  

  • アジアの食と腸内フローラの関係 招待

    中山二郎

    第28回西日本食品産業創造展  2018年5月 

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    開催年月日: 2018年5月

    記述言語:日本語   会議種別:シンポジウム・ワークショップ パネル(公募)  

    開催地:マリンメッセ福岡   国名:日本国  

  • 世界の健康に貢献する日本食の特徴とは 腸内細菌叢研究から見た日本人の食生活と健康 Asian Microbiome Projectの研究成果から

    中山 二郎, Lee Yuan-Kun

    日本栄養・食糧学会大会講演要旨集  2018年4月  (公社)日本栄養・食糧学会

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    開催年月日: 2018年4月

    記述言語:日本語  

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  • アジア人の腸内細菌叢の実態調査 招待

    中山二郎

    第26回アジア栄養科学ワークショップ「腸内細菌と健康」  2018年1月 

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    開催年月日: 2018年1月

    記述言語:日本語   会議種別:シンポジウム・ワークショップ パネル(公募)  

    開催地:中村学園大学   国名:日本国  

  • In vitroにおけるセファゾリンとナイシンAの乳房炎原因菌に対する相乗的殺菌効果

    北崎宏平, 北崎宏平, 古賀祥子, 永利浩平, 桑野剛一, 善藤威史, 中山二郎, 園元謙二, 片本宏

    日本産業動物獣医学会(九州)・日本小動物獣医学会(九州)・日本獣医公衆衛生学会(九州)  2018年 

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    開催年月日: 2018年

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  • 日本人の腸内フローラ 招待

    中山二郎

    発酵食品研究の最前線  2017年11月 

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    開催年月日: 2017年11月

    記述言語:日本語   会議種別:シンポジウム・ワークショップ パネル(公募)  

    開催地:広島大学生物生産学部   国名:日本国  

  • 海洋深層水の微生物群集構造と放線菌分離株の抗癌活性について

    今田 千秋, 梁 太煕, 山田 勝久, 中山 二郎, 寺原 猛, 小林 武志

    日本放線菌学会大会講演要旨集  2017年9月  日本放線菌学会

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    開催年月日: 2017年9月

    記述言語:日本語  

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  • 日本人の腸内胆汁酸プロファイルと腸内細菌叢の関連解析

    余田美沙子、田中優、内川彩夏、本田倫子、百田理恵、池田温子、河口礼佳、園元謙二、十倉充範、中山二郎

    化学関連支部合同九州大会2017  2017年7月 

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    開催年月日: 2017年7月

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:北九州国際会議場   国名:日本国  

  • Asian Microbiome Project:アジア人腸内細菌叢の実態調査

    中山二郎

    第2回デザイン生命工学研究会大会  2017年3月 

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    開催年月日: 2017年3月

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:神戸大学   国名:日本国  

  • グルコシルセラミドによる腸内細菌叢への影響の解析

    佐藤友哉、加藤曖菜、浜島弘史、中山二郎、北垣浩志

    日本生物工学会九州支部大会  2016年12月 

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    開催年月日: 2016年12月

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:九州工業大学情報工学部   国名:日本国  

  • ヒト腸内細菌叢のダイナミズムとダイバーシティー

    中山 二郎

    日本乳酸菌学会誌  2016年11月  日本乳酸菌学会

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    開催年月日: 2016年11月

    記述言語:日本語  

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  • 麴グルコシルセラミドの腸内細菌叢への影響 招待

    藤川彩美、佐藤友哉、浜島弘史、柳田晃良、光武進、永尾晃治、中山二郎、北垣浩志

    日本生物工学会  2016年9月 

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    開催年月日: 2016年9月

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:富山国際会議場   国名:日本国  

  • Clostridium perfringensの毒素発現を制御する内在性クオラムクエンチングの実態解明

    安達桂香、Ravindra Pal Singh,大谷郁、園元謙二、中山二郎

    日本生物工学大会  2016年9月 

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    開催年月日: 2016年9月

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:富山国際会議場   国名:日本国  

  • Clostridium属細菌のagr様クオラムセンシング系における自己誘導ペプチドの検出

    神川美樹、安達桂香、大久保謙一、大谷郁、岡健太郎、高橋志達、園元謙二、中山二郎

    日本農芸化学会西日本支部大会  2016年9月 

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    開催年月日: 2016年9月

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:長崎大学文教キャンパス   国名:日本国  

  • 和食の機能性のメカニズム 生活習慣病予防、腸内細菌へのインパクト 麹グルコシルセラミドの腸内細菌叢への影響

    藤川 彩美, 佐藤 友哉, 浜島 弘史, 柳田 晃良, 光武 進, 永尾 晃治, 中山 二郎, 北垣 浩志

    日本生物工学会大会講演要旨集  2016年8月  (公社)日本生物工学会

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    開催年月日: 2016年8月

    記述言語:日本語  

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  • 環状バクテリオシン,ロイコサイクリシンQの生合成に関与するDUF95スーパーファミリータンパク質の機能(2015生物工学論文賞)

    Mu Fuqin, 益田 時光, 善藤 威史, 小野 浩, 北川 博史, 伊藤 晴夫, 中山 二郎, 園元 謙二

    生物工学会誌 : seibutsu-kogaku kaishi  2016年  日本生物工学会

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    開催年月日: 2016年

    記述言語:日本語  

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  • 標題・氏名(所属)麹グリコシルセラミドはプレバイオティクスとして機能する

    藤川彩美, 浜島弘史, 佐藤友哉, 柳田晃良, 光武進, 永尾晃治, 中山二郎, 北垣浩志

    日本醸造学会大会講演要旨集  2016年 

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    開催年月日: 2016年

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  • 日本各地の海洋深層水中の細菌群集構造解析

    寺原猛, 梁太熈, 小林武志, 山田勝久, 今田千秋, 中山二郎, 五十嵐康弘, 池上康之

    海洋深層水研究  2016年 

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    開催年月日: 2016年

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  • 日本各地で採水された海洋深層水中の細菌群集組成解析

    寺原猛, 梁太熈, 中山二郎, 五十嵐康弘, 池上康之, 小林武志, 山田勝久, 今田千秋

    マリンバイオテクノロジー学会大会講演要旨集  2016年 

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    開催年月日: 2016年

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  • 和食に共通して含まれる麹グリコシルセラミドが腸内細菌叢に及ぼす影響の解析

    佐藤友哉, 譚政, 中山二郎, 浜島浩史, 光武進, 永尾晃治, 北垣浩志

    日本農芸化学会大会講演要旨集(Web)  2016年 

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    開催年月日: 2016年

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  • アジア人の食と腸内細菌と健康に関するマルチコホート研究

    中山 二郎

    松本歯学  2015年12月  松本歯科大学学会

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    開催年月日: 2015年12月

    記述言語:日本語  

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  • 発酵温度および塩分濃度が糠床菌叢に及ぼす影響

    小野 浩, 西尾 翔子, 釣井 隼, 河本 哲宏, 園元 謙二, 中山 二郎

    日本生物工学会大会講演要旨集  2015年9月  (公社)日本生物工学会

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    開催年月日: 2015年9月

    記述言語:日本語  

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  • Asian Microbiome Project:アジア人の食と腸内フローラと健康に関する横断型コホート研究 招待

    中山二郎、東佳那子、山本麻寿紗、百田理恵、本田倫子、渡辺幸一、Yuan Kun Lee

    日本農芸化学会2015年度大会  2015年3月 

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    開催年月日: 2015年3月

    記述言語:日本語   会議種別:シンポジウム・ワークショップ パネル(公募)  

    開催地:岡山・岡山大学   国名:日本国  

  • Asian microbiome project phase II: アジア人の腸内フローラの加齢による変化

    山本麻寿紗、東佳那子、百田理恵、本田倫子、渡辺幸一、Shirchin Demberel、Sunee Nitisinprasert、Park Yong Ha、Endang S. Rahayu、Fa-Zheng Ren、Yuan Kun Lee、園元謙二、中山二郎

    日本農芸化学会2015年度大会  2015年3月 

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    開催年月日: 2015年3月

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:岡山・岡山大学   国名:日本国  

  • Asian Microbiome Project: アジア人の腸内細菌叢に関する横断型コホート研究 招待

    中山二郎、東佳那子、山本麻寿紗、本田倫子、百田理恵、園元謙二、Yuan Kun Lee

    第88回日本細菌学会総会  2015年3月 

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    開催年月日: 2015年3月

    記述言語:日本語   会議種別:シンポジウム・ワークショップ パネル(公募)  

    開催地:岐阜・長良川国際会議場   国名:日本国  

  • Asian Microbiome Project: Diversity in gut microbiota of Asian people 招待 国際会議

    Jiro Nakayama, Yuan Kun Lee

    4th Beneficial Microbes Conference  2015年3月 

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    開催年月日: 2015年3月

    記述言語:英語   会議種別:口頭発表(一般)  

    開催地:オランダ・ハーグ   国名:オランダ王国  

  • 次世代シーケンサーによる日本各地の海洋深層水中の微生物群集構造解析

    梁太煕, 山田勝久, 中山二郎, 五十嵐康弘, 寺原猛, 小林武志, 今田千秋

    マリンバイオテクノロジー学会大会講演要旨集  2015年 

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    開催年月日: 2015年

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  • Asian Microbiome Project:アジア人の腸内細菌叢に関する調査研究 招待

    中山二郎

    日本食品免疫学会第9回宿泊セミナー  2014年12月 

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    開催年月日: 2014年12月

    記述言語:日本語   会議種別:公開講演,セミナー,チュートリアル,講習,講義等  

    開催地:東京・水月ホテル鴎外荘   国名:日本国  

  • Identification of autoinducing peptide of Clostridium perfringens and development of its inhibitors 国際会議

    Ravindra Pal Singh, Kaori Ohtani, Ryoji Yokohata, Kenji Sonomoto, Toru Shimizu, Jiro Nakayama

    5th AMS conference on cell-cell communication in bacteria  2014年10月 

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    開催年月日: 2014年10月

    記述言語:英語   会議種別:口頭発表(一般)  

    開催地:San Antonio   国名:アメリカ合衆国  

  • Asian microbiome project phase II: アジア人の腸内フローラの加齢による変化

    中山二郎ら

    第18回腸内細菌学会  2014年6月 

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    開催年月日: 2014年6月

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:東京・東京大学   国名:日本国  

  • Hamigera属糸状菌が生産するクオラムセンシング阻害物質に関する研究

    門嶋泰斗, 郷田史也, 花房知朗, 松藤貴久, 中山二郎, 五十嵐康弘

    日本農芸化学会2014年度大会  2014年3月 

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    開催年月日: 2014年3月

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:東京   国名:日本国  

  • 細菌の環境シグナル受容体と遺伝子調節ネットワーク ウェルシュ菌Clostridium perfringensの細胞間および細胞内のシグナル伝達による遺伝子発現調節(Bacterial signal receptors and networks for gene regulation Gene regulation by inter- and intra-cellular signaling in Clostridium perfringens)

    大谷 郁, 中山 二郎, 清水 徹

    日本細菌学雑誌  2014年2月  日本細菌学会

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    開催年月日: 2014年2月

    記述言語:英語  

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  • Diversity of intestinal microbiota in Asian younsters profiled by 16S rRNA pyrosequencing

    Jiang J, Nakayama J, Watanabe K, Sakamoto N, Matsuda K, Kurakawa T, Tsuji H, Ren FZ, Nitisinprasert S, Rahayu ES, Liao CC, Tsai YC, Lee YK

    第35回日本分子生物学会  2012年12月 

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    開催年月日: 2012年12月

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:福岡   国名:日本国  

  • 腸球菌のクオラムセンシング阻害剤YAA5911の誘導体化と活性評価

    松藤貴久,横畑綾治,園元謙二,中山二郎

    第49回化学関連支部合同九州大会  2012年6月 

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    開催年月日: 2012年6月

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:北九州   国名:日本国  

  • 腸球菌におけるGelatinase Biosynthesis Activating Pheromone (GBAP)の生合成機構解析

    北川はるか,横畑綾治,佐土原理江,園元謙二,中山二郎

    第19回九州支部大分大会  2012年6月 

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    開催年月日: 2012年6月

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:別府   国名:日本国  

  • 乳幼児における腸内細菌叢とアレルギー発症の関連性に関するコホート調査研究

    伊佐佳菜子,是則有希,鷲尾昌一,小林貴子,清原千香子,古賀泰裕,三上克央,柴田瑠美子,園元謙二,中山二郎

    腸内細菌学会  2012年6月 

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    開催年月日: 2012年6月

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:神戸   国名:日本国  

  • オミックス解析技術の裏技紹介 : 生物工学分野への応用

    榊原 陽一, 馬場 健史, 中山 二郎

    生物工学会誌 : seibutsu-kogaku kaishi  2011年11月  日本生物工学会

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    開催年月日: 2011年11月

    記述言語:日本語  

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  • BACTERIOCINS OF LACTIC ACID BACTERIA: NEW APPLICATIONS AND PRODUCTION 招待 国際会議

    Kenji Sonomoto, Takeshi Zendo, Jiro Nakayama

    International Union of Microbiological Societies 2011 Congress  2011年9月 

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    開催年月日: 2011年9月

    記述言語:英語   会議種別:口頭発表(一般)  

    開催地:札幌   国名:日本国  

  • 乳酸菌によるビブリオ属細菌の感染阻害に関する研究

    鎌田陽菜, 清水耕平, 松永尚之, 藤岡秀幸, 徳永遼平, 岩谷駿, 中山二郎, 沖野望, 伊東信

    日本水産学会大会講演要旨集  2011年 

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    開催年月日: 2011年

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  • バイエル板樹状細胞の微生物成分刺激に対する応答性の解析

    輪島隼一, 能川佳子, 塩河亜弥, 三坂祐未, 小川晋平, 佐藤隆一郎, 中山二郎, 八村敏志

    日本農芸化学会大会講演要旨集  2010年 

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    開催年月日: 2010年

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  • SS4-2 乳児期の腸内フローラ形成と後のアレルギー発症の関連性に関する調査研究(III,食餌習慣・腸内細菌とアレルギー~プロバイオティクス・バイオジェニックス~,生活環境習慣病としてのアレルギーを検証する,第59回日本アレルギー学会秋季学術大会)

    中山 二郎

    アレルギー  2009年  一般社団法人 日本アレルギー学会

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    開催年月日: 2009年

    記述言語:日本語  

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  • Quorum-Sensingをめぐる最近の話題 腸球菌のクオーラムセンシングとその応用

    中山 二郎

    薬剤耐性菌シンポジウム講演記録  2003年8月  薬剤耐性菌研究会

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    開催年月日: 2003年8月

    記述言語:日本語  

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  • 乳酸菌の多様な新機能を求めて

    麻生 祐司, 永尾 潤一, 中山 二郎, 園元 謙二

    日本農芸化学会誌  2003年8月  (公社)日本農芸化学会

  • 新たに分離したLactococcus lactisが生産する新規バクテリオシン

    善藤 威史, 深尾 匡憲, 古賀 祥子, 冨士田 浩二, 中山 二郎, 園元 謙二

    日本乳酸菌学会誌  2003年6月  日本乳酸菌学会

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    開催年月日: 2003年6月

    記述言語:日本語  

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  • 1S1-PM6 ランチビオティック nukacin ISK-1 : プラスミドの全塩基配列解析から生合成・自己耐性の分子メカニズムまで

    麻生 祐司, 永尾 潤一, 金政 庸平, 古賀 華子, Nguyen Thi Bich Phuong, 指原 紀宏, 中山 二郎, 園元 謙二

    日本生物工学会大会講演要旨集  2003年  日本生物工学会

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    開催年月日: 2003年

    記述言語:日本語  

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  • 耐塩性乳酸菌Tetragenococcus halophilusの分子シャペロンDnaKの機能解析

    杉本真也, 福田大介, 曽根崎志野, JATURAPAT A, 中山二郎, 園元謙二

    日本農芸化学会大会講演要旨集  2003年 

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    開催年月日: 2003年

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  • ランチビオティック,nukacin ISK-1生合成タンパク質複合体の解析

    永尾潤一, 麻生祐司, 指原紀宏, 中山二郎, 園元謙二

    日本農芸化学会大会講演要旨集  2003年 

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    開催年月日: 2003年

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  • Lactococcus lactis QU5株が生産するバクテリオシン

    冨士田浩二, 一政士郎, 古賀祥子, 善藤威史, 中山二郎, 園元謙二

    日本栄養・食糧学会西日本支部大会プログラム・講演要旨集  2003年 

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    開催年月日: 2003年

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  • eDNAに含まれる二次代謝産物生合成遺伝子の利用

    作田庄平, 中山二郎, 河内隆, 岡本晋, 松田智子, 上迫和也, 長澤寛道

    日本放線菌学会大会講演要旨集  2003年 

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    開催年月日: 2003年

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  • 2D14-3 Enterococcus faecium NKR-5-3 が生産する多成分バクテリオシンの解析

    冨士田 浩二, Wilaipun Pongtep, 善藤 威史, Leelawatchara Vichien, 中山 二郎, 園元 謙二

    日本生物工学会大会講演要旨集  2003年  日本生物工学会

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    開催年月日: 2003年

    記述言語:日本語  

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  • 新たに分離された乳酸菌が生産するバクテリオシン

    善藤 威史, 古賀 祥子, Nitisinprasert Sunee, Wilaipun Pongtep, Swetwiwathana Adisorn, 中山 二郎, 園元 謙二

    日本乳酸菌学会誌  2002年6月  日本乳酸菌学会

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    開催年月日: 2002年6月

    記述言語:日本語  

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  • 506 耐塩性乳酸菌Tetragenococcus halofphilaの分子シャペロンDnaKの機能解析(酵素・酵素工学・タンパク質工学,一般講演)

    杉本 真也, 福田 大介, 渡邊 麻希, 曽根崎 志野, 中山 二郎, 園元 謙二

    日本生物工学会大会講演要旨集  2002年  日本生物工学会

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    開催年月日: 2002年

    記述言語:日本語  

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  • 耐塩性乳酸菌Tetragenococcus halophilaの分子シャペロンDnaKの機能解析

    曾根崎志野, 福田大介, 中山二郎, 園元謙二

    日本農芸化学会大会講演要旨集  2002年 

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    開催年月日: 2002年

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  • 新しい食品のバイオ技術と安全確保技術 乳酸菌バクテリオシンの生産と食品への応用技術

    園元謙二, 善藤威史, 中山二郎

    日本生物工学会大会講演要旨集  2002年 

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    開催年月日: 2002年

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  • 大豆から分離した乳酸菌Enterococcus mundtii alが生産するバクテリオシンの遺伝生化学的解析

    野村久美, 藤岡澄江, 善藤威史, 小林元太, 中山二郎, 園元謙二

    日本農芸化学会大会講演要旨集  2002年 

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    開催年月日: 2002年

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  • 乳酸菌の世界における細胞間コミュニケーション シグナル伝達分子としてのペプチドフェロモン

    中山二郎, 園元謙二

    日本農芸化学会大会講演要旨集  2002年 

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    開催年月日: 2002年

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  • ランチビオティックnukacin ISK-1生合成遺伝子群の解析

    麻生祐司, 指原紀宏, 中山二郎, 園元謙二

    日本農芸化学会大会講演要旨集  2002年 

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    開催年月日: 2002年

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  • ポスト食をめざす乳酸菌研究 乳酸菌の細胞間コミュニケーションと環境応答

    中山二郎, 園元謙二

    日本生物工学会大会講演要旨集  2002年 

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    開催年月日: 2002年

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  • トウモロコシからのバクテリオシン生産乳酸菌の分類

    古賀祥子, 野村久美, 善藤威史, 中山二郎, 園元謙二

    日本農芸化学会大会講演要旨集  2002年 

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    開催年月日: 2002年

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  • タイの発酵食品,Pla-raから分離した乳酸菌が生産するバクテリオシン

    WILAIPUN P, 善藤威史, LEELAWATCHARAMAS V, 中山二郎, 園元謙二

    日本農芸化学会大会講演要旨集  2002年 

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    開催年月日: 2002年

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  • わが国における乳酸菌フロンティア研究 乳酸菌の世界における細胞間コミュニケーション

    中山二郎, 園元謙二

    日本農芸化学会誌  2002年 

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    開催年月日: 2002年

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  • ぬか床中に棲息する微生物の多様性解析

    安藤瑞樹, 福田大介, 黒木佑美子, 中山二郎, 園元謙二

    日本農芸化学会大会講演要旨集  2002年 

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    開催年月日: 2002年

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  • S904 乳酸菌の細胞間コミュニケーションと環境応答(ポスト食をめざす乳酸菌研究,シンポジウム)

    中山 二郎, 園元 謙二

    日本生物工学会大会講演要旨集  2002年  日本生物工学会

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    開催年月日: 2002年

    記述言語:日本語  

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  • S110 乳酸菌バクテリオシンの生産と食品への応用技術(新しい食品のバイオ技術と安全確保技術,シンポジウム)

    園元 謙二, 善藤 威史, 中山 二郎

    日本生物工学会大会講演要旨集  2002年  日本生物工学会

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    開催年月日: 2002年

    記述言語:日本語  

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  • Nukacin ISK-1生合成に関与するタンパク質NukM,NukTの発現および機能解析

    永尾潤一, 河村嘉奈, 麻生祐司, 指原紀宏, 中山二郎, 園元謙二

    日本農芸化学会大会講演要旨集  2002年 

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    開催年月日: 2002年

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  • nukacin ISK-1オペロンに存在する機能不明なorf1,orf7の機能解析

    金政庸平, 橋本拓, 麻生祐司, 指原紀宏, 中山二郎, 園元謙二

    日本農芸化学会大会講演要旨集  2002年 

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    開催年月日: 2002年

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  • 708 植物からの新奇バクテリオシン生産乳酸菌の探索(生物情報工学,発酵・発酵工学,一般講演)

    善藤 威史, 古賀 祥子, 瀬戸 紘美, 中山 二郎, 園元 謙二

    日本生物工学会大会講演要旨集  2002年  日本生物工学会

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    開催年月日: 2002年

    記述言語:日本語  

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  • 腸球菌のプロテアーゼ生産におけるクオーラムセンシングを制御するgelatinase biosynthesis activating pheromone(GBAP)

    中山二郎, 曹庸, 堀井孝昭, 作田庄平, 長沢寛道

    日本農芸化学会誌  2001年 

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    開催年月日: 2001年

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  • 難培養性微生物を含む土壌由来,新規II型ポリケタイド生合成酵素遺伝子の探索

    河内隆, 上迫和也, 岡本晋, 中山二郎, 作田庄平, 長沢寛道

    日本農芸化学会誌  2001年 

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    開催年月日: 2001年

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  • 難培養性土壌細菌群が有するポリケチド生合成遺伝子の多様性および新規性の解析

    中山二郎, 松田智子, 上迫和也, 河内隆, 岡本晋, 作田庄平, 長沢寛道

    日本農芸化学会誌  2001年 

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    開催年月日: 2001年

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  • 腸球菌の性フェロモンシグナリングに関する生物有機化学的・分子生物学的研究

    中山二郎

    日本農芸化学会関東支部講演要旨集  2000年 

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    開催年月日: 2000年

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  • Enterococcus faecal is の性フェロモン受容体タンパク質TraAの機能解析

    堀井 孝昭, 中山 二郎, 作田 庄平, 長澤 寛道

    日本乳酸菌学会誌 = Journal of Japan Society for Lactic Acid Bacteria  1998年9月 

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    開催年月日: 1998年9月

    記述言語:日本語  

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  • Enterococcus faecalisにおける性フェロモンの受容認識機構の解析-その2

    中山 二郎, 高浪 雄一郎, 堀井 孝昭, 鈴木 昭憲

    日本乳酸菌学会誌 = Journal of Japan Society for Lactic Acid Bacteria  1997年9月 

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    開催年月日: 1997年9月

    記述言語:日本語  

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  • 40 アフラトキシン生産阻害物質aflastatinに関する研究(口頭発表の部)

    作田 庄平, 池田 博幸, 櫻田 賢, 降旗 一夫, 中山 二郎, 鈴木 昭憲, 小野 誠, 磯貝 彰

    天然有機化合物討論会講演要旨集  1997年  天然有機化合物討論会実行委員会

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    開催年月日: 1997年

    記述言語:日本語  

    A novel inhibitor of aflatoxin production of Aspergillus parasiticus was isolated from the mycelial extracts of Streptomyces sp. MRI142 and termed aflastatin A (1). The molecular formula of 1 was determined as C_<62>H_<115>NO_<24>, and its structure was elucidated by NMR and chemical degradation experiments. By analyzing a variety of 2D-NMR spectra of 1, a partial polyhydroxylated structure (A) was identified. Based on the structure of A, 1 was oxidized with NaIO_4, and three fragment molecules, 2, 3 and 4, were obtained. Since all carbon atoms contained in 1 were involved in the partial and fragment structures, its carbon chain was constructed with them. Finally, the stereochemistry of a tetrahydropyran ring was determined by J values and NOEs among the ring protons. Thus, the total structure of aflastatin A was characterized as 1, which is a unique tetramic acid derivative having a highly oxygenated long alkyl chain. To determine the absolute configuration of 4, 2,4-dimethyl-1,6-hexanediol dibenzoate (6) was prepared from 4. By comparison of its CD spectrum with those of opticaly active (2R, 4S)- and (2S, 4S)-6, which were derivatized from cycloheximide, the configurations of natural 6 were determined to (2S, 4R). On the other hand, N-methylalanine from 4 was determined to have a D configuration. The incorporation experiments using ^<13>C-labeled acetate and propionate suggested that most of the C_2 and C_3 units involved in the alkyl chain moiety of 1 were biosynthesized from acetic and propionic acid. Unexpectedly, no incorporation was observed in one of the C_2 units and two of the C_4 units in the alkyl chain. Aflastatin A inhibited aflatoxin production in A. parasiticus at the concentration of 0.5μg/mL completely, but didn't inhibit its growth at the same concentration.

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  • Enterococcus faecalisにおける性フェロモンの受容認識機構の解析

    中山 二郎, 高浪 雄一郎, 鈴木 昭憲

    乳酸菌研究集談会誌  1996年11月 

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    開催年月日: 1996年11月

    記述言語:日本語  

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  • Enterococcus faecalisにおける性フェロモンの受容認識機構の解析 : 微生物

    中山 二郎, 小野 幸胤, 高浪 雄一郎, 阿部 有生, 大城 紀子, 鈴木 昭憲

    日本農藝化學會誌  1996年3月  社団法人日本農芸化学会

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    開催年月日: 1996年3月

    記述言語:日本語  

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  • カイコ体液由来麻痺ペプチドの構造決定 : 有機化学・天然物化学

    河 順得, 中山 二郎, 永田 晋治, 鈴木 昭憲, 片岡 広誌

    日本農藝化學會誌  1996年3月  社団法人日本農芸化学会

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    開催年月日: 1996年3月

    記述言語:日本語  

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  • Enterococcus faecalisのプラスミド接合伝達を誘導する性フェロモン及びそのインヒビターの構造解析 : 微生物

    阿部 有生, 中山 二郎, 高浪 雄一郎, 鈴木 昭憲

    日本農藝化學會誌  1995年7月  社団法人日本農芸化学会

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    開催年月日: 1995年7月

    記述言語:日本語  

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  • Enterococcus faecalisのプラスミドpPD1の性フェロモン感知(traC)およびシャットダウン(traB)遺伝子の解析

    中山二郎, 磯貝彰, 鈴木昭憲

    日本農芸化学会誌  1994年 

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    開催年月日: 1994年

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  • 細菌の性フェロモン

    中山二郎

    日本農芸化学会関東支部講演要旨集  1994年 

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    開催年月日: 1994年

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  • Streptococcus faecalisの性フェロモン誘導性細胞表層蛋白質に関する研究(微生物-構造と機能-)

    中山 二郎, 長澤 寛道, 磯貝 彰, 坂神 洋次, 森 正明, 石井 ゆかり, D.B Clewell, 鈴木 昭憲

    日本農藝化學會誌  1988年3月  社団法人日本農芸化学会

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    開催年月日: 1988年3月

    記述言語:日本語  

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  • 乳酸菌が生産する新奇抗菌性ペプチド、ラクティシンQの選択毒性の解明

    大野 香菜子、米山 史紀、善藤 威史、中山 二郎、園元 謙二

    日本農芸化学会  2008年3月 

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    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:名古屋・名城大学   国名:日本国  

  • 乳児期における腸内フローラ形成とプロバイオティクス 招待

    中山二郎

    西日本食品産業創造展  2008年5月 

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    記述言語:日本語   会議種別:シンポジウム・ワークショップ パネル(公募)  

    開催地:福岡   国名:日本国  

  • ピロシーケンス法を用いた乳幼児期腸内細菌叢モニタリングシステムの構築とプロバイオティクス投与の影響の解析

    中山二郎、手島幹子、田中重光、小林貴子、坪内美樹、清原千香子、白川太郎、園元謙二

    腸内細菌学会  2008年6月 

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    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:東京   国名:日本国  

  • 腸球菌のクォーラムセンシングを制御する環状ペプチドGBAPのin vitroにおける生合成系の構築

    吉村 憲人、西口 賢三、上村結美、中山 二郎、園元 謙二

    化学関連支部合同九州大会  2008年7月 

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    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:北九州   国名:日本国  

  • 乳児期のプロバイオティクス投与が腸内フローラ形成に与える影響

    手島幹子、小林貴子、田中重光、坪内美樹、清原千香子、白川太郎、園元謙二、中山二郎

    日本農芸化学会大会2008年度西日本支部大会  2008年9月 

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    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:長崎   国名:日本国  

  • Development of inhibitors targeting cyclic peptide-mediated quorum sensing in Gram-positive bacteria

    Jiro Nakayama, Kenzo Nishiguchi, Yumi Uemura, Emi Tanaka, kenji Sonomoto

    Japan-Netherlands Joint Seminar on Enzyme Science and Biotechnology  2008年9月 

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    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:福岡   国名:日本国  

  • 乳児期の腸内フローラの群衆構造と宿主免疫系への影響—次世代高速シーケンサーを利用したフローラ解析法の導入 招待

    中山二郎

    FIMF合同フォーラム2008  2008年11月 

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    記述言語:日本語   会議種別:シンポジウム・ワークショップ パネル(公募)  

    開催地:東京   国名:日本国  

  • 腸球菌のquorum sensingを制御する環状ペプチドフェロモンGBAPの生合成に関与する膜タンパク質FsrB'の機能解析

    吉村 憲人、西口 賢三、軸屋 博之、園元 謙二、中山 二郎

    日本生物工学会九州支部大会  2008年12月 

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    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:熊本   国名:日本国  

  • グラム陽性菌の環状ペプチドをオートインデューサーとするクオラムセンシングの阻害剤開発 招待

    中山二郎

    日本分子生物学会年会 日本生化学会大会 合同大会  2008年12月 

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    記述言語:日本語   会議種別:シンポジウム・ワークショップ パネル(公募)  

    開催地:神戸   国名:日本国  

  • ピロシーケンス法を用いた腸内細菌叢解析システムの構築

    中山二郎、手島幹子、小林貴子、阪本直茂、園元謙二

    日本生物工学会  2009年1月 

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    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:名古屋   国名:日本国  

  • グラム陽性細菌のクオラムセンシング研究の最前線 招待

    中山二郎

    2009年度日本乳酸菌学会秋期セミナー  2009年1月 

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    記述言語:日本語  

    開催地:東京   国名:日本国  

  • 乳児期の腸内フローラ形成と後のアレルギー発症の関連性に関する調査研究 招待

    中山二郎

    第59回日本アレルギー学会秋季学術大会特別シンポジウム  2009年1月 

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    記述言語:日本語  

    開催地:秋田   国名:日本国  

  • 乳幼児期の腸内フローラの形成と アレルギー発症との関連性について 招待

    中山二郎

    第63回日本栄養・食糧学会シンポジウム  2009年1月 

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    記述言語:日本語  

    開催地:長崎   国名:日本国  

  • 乳児期の腸内細菌叢と後のアレルギー発症の関連性に関する前向き調査:ピロシーケンス法による菌叢データの利用

    小林貴子、手島幹子、田中重光、清原千香子、園元謙二、中山二郎

    日本農芸化学会  2009年3月 

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    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:福岡   国名:日本国  

  • Weissella hellenica QU 13が生産する新奇乳酸菌バクテリオシンの諸特性と構造解析

    益田 時光、善藤 威史、中山 二郎、園元 謙二

    日本農芸化学会  2009年3月 

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    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:福岡   国名:日本国  

  • 耐熱性乳酸菌Lactobacillius salivarius AC21 が生産するバクテリオシン、salivacin K21の分子解析

    古賀 千恵、NITISINPRASERT Sunee、善藤 威史、中山 二郎、園元 謙二、松崎 弘美

    日本農芸化学会  2009年3月 

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    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:福岡   国名:日本国  

  • Enterococcus faecium NKR-5-3によるバクテリオシンの生産制御

    姫野 康平、PEREZ Rodney、冨士田 浩二、善藤 威史、WILAIPUN Pongtep、中山 二郎、園元 謙二

    日本農芸化学会  2009年3月 

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    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:福岡   国名:日本国  

  • Enterococcus faecium NKR-5-3が生産するバクテリオシン群の生合成遺伝子の解析

    石橋 直樹、姫野 康平、益田 時光、冨士田 浩二、善藤 威史、WILAIPUN Pongtep、中山 二郎、園元 謙二

    日本農芸化学会  2009年3月 

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    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:福岡   国名:日本国  

  • Lactococcus sp. QU 12が生産する新奇環状バクテリオシン、lactocyclicin Q

    澤 稔彦、善藤 威史、清藤 順子、冨士田 浩二、中山 二郎、園元 謙二

    日本農芸化学会  2009年3月 

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    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:福岡   国名:日本国  

  • 糠床中の複合微生物系における難培養性乳酸菌Lactobacillus acetotoleransの動態解析

    阪本 直茂、園元 謙二、中山 二郎

    日本農芸化学会  2009年3月 

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    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:福岡   国名:日本国  

  • 乳酸菌バクテリオシン、ラクティシンQの脂質依存的な抗菌活性

    大野 香菜子、米山 史紀、善藤 威史、中山 二郎、松崎 勝巳、園元 謙二

    日本農芸化学会  2009年3月 

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    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:福岡   国名:日本国  

  • 乳酸菌バクテリオシン、ラクティシンQの巨大な孔形成を伴う作用機構

    善藤 威史、米山 史紀、井村 雄一、大野 香菜子、中山 二郎、松崎 勝巳、園元 謙二

    日本農芸化学会  2009年3月 

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    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:福岡   国名:日本国  

  • 腸球菌のクオラムセンシングを制御する環状ペプチドフェロモンGBAPの生合成機構の解明

    吉村 憲人、西口 賢三、軸屋 博之、園元 謙二、中山 二郎

    日本農芸化学会  2009年3月 

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    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:福岡   国名:日本国  

  • 腸球菌のクォーラムセンシングを制御する環状ペプチドフェロモン(GBAP)の構造および機能解析

    西口 賢三、永田 宏次、佐藤 まみ、田之倉 優、園元 謙二、中山 二郎

    日本農芸化学会  2009年3月 

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    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:福岡   国名:日本国  

  • L. plantarum WCFS1のlamクオラムセンシングを制御するクオルモンLamD558の同定

    朱雀 奈津子、藤井 敏雄、善藤 威史、西口 賢三、de Vos Willem、園元 謙二、中山 二郎

    日本農芸化学会  2009年3月 

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    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:福岡   国名:日本国  

  • 乳酸菌Lactococcus lactisが保持する分子シャペロンDnaKのin vivo機能解析

    WILAIPUN Pongtep、善藤 威史、奥田 賢一、中山 二郎、園元 謙二

    日本農芸化学会  2009年3月 

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    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:福岡   国名:日本国  

  • ランチビオティック耐性ABCトランスポーターに存在するE-loopの機能的重要性

    柳原 沙恵、奥田 賢一、中山 二郎、園元 謙二

    日本農芸化学会  2009年3月 

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    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:福岡   国名:日本国  

  • ランチビオティック吸着型耐性タンパク質NukHの吸着特異性の解析

    奥田 賢一、柳原 沙恵、中山 二郎、園元 謙二

    日本農芸化学会  2009年3月 

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    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:福岡   国名:日本国  

  • 乳酸菌利用による有用物質(抗菌ペプチド)の医療応用 招待

    永利 浩平、林 いずみ、竹花 稔彦、善藤 威史、中山 二郎、園元 謙二

    日本農芸化学会  2009年3月 

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    記述言語:日本語   会議種別:シンポジウム・ワークショップ パネル(公募)  

    開催地:福岡   国名:日本国  

  • グラム陽性菌のクオラムセンシング阻害剤の開発と展望 招待

    中山 二郎、西口 賢三、永田 宏次、田中 笑美、上村 結美、吉村 憲人、五十嵐 康弘、園元 謙二

    日本農芸化学会2009年度大会シンポジウム  2009年3月 

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    記述言語:日本語  

    開催地:福岡   国名:日本国  

    Status of Recent Research in Probiotic used as an additive to livestock. Annual meeting of The Japanese Society for Bioscience, Biotechnology and Agro-Chemistry

  • リバースアラニンスキャン法による腸球菌のクオルモン‘GBAP’のアンタゴニスト創製

    佐藤 まみ、中山 二郎、西口 賢三、園元 謙二

    日本農芸化学会  2010年1月 

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    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:東京   国名:日本国  

  • ナイシンを利用した酪農分野における牛感染症防除 招待

    北崎 宏平、馬場 武志、古賀 康弘、桑野 剛一、福田 浩章、河田 恵美、高巣 祐介、竹花 稔彦、古賀 祥子、永利 浩平、島田 信也、農  新介、林 龍鶴、前田 幸子、川崎 貞道、善藤 威史、中山 二郎、園元 謙二

    日本農芸化学会  2010年1月 

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    記述言語:日本語   会議種別:シンポジウム・ワークショップ パネル(公募)  

    開催地:東京   国名:日本国  

  • ランチビオティック:分子内環化翻訳後修飾およびユニークな機能 招待

    園元 謙二、西江 麻美、永尾 潤一、善藤 威史、中山 二郎

    日本農芸化学会  2010年1月 

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    記述言語:日本語   会議種別:シンポジウム・ワークショップ パネル(公募)  

    開催地:東京   国名:日本国  

  • nukacin ISK-1の抗菌活性における必須および可変残基の機能評価

    Mohammad R. Islam、佐々木 信、塩屋 幸樹、永尾 潤一、西江 麻美、軸屋 博之、善藤 威史、中山 二郎、園元 謙二

    日本農芸化学会  2010年1月 

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    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:東京   国名:日本国  

  • Lactococcus lactisが生産する新奇バクテリオシン、lacticin Q/Z生合成遺伝子群の比較と機能解析

    岩谷 駿、米山 史紀、宮下 紫穂、善藤 威史、中山 二郎、園元 謙二

    日本農芸化学会  2010年1月 

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    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:東京   国名:日本国  

  • 新奇乳酸菌バクテリオシンweissellicinY,Mの生合成機構に関する研究

    益田 時光、JIMENES Juan Jose、善藤 威史、中山 二郎、園元 謙二

    日本農芸化学会  2010年1月 

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    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:東京   国名:日本国  

  • Enterocin A, B生産菌Enterococcus faecium KU-B5が生産する新奇二成分バクテリオシンenterocin X

    胡 智柏、MALAPHAN Wanna、善藤 威史、中山 二郎、園元 謙二

    日本農芸化学会  2010年1月 

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    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:東京   国名:日本国  

  • パイエル板樹状細胞の微生物成分刺激に対する応答性の解析

    輪島 隼一、能川 佳子、塩河 亜弥、三坂 祐未、小川 晋平、佐藤 隆一郎、中山 二郎、八村 敏志

    日本農芸化学会  2010年1月 

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    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:東京   国名:日本国  

  • 腸球菌のクオーラムセンシング自己誘導因子GBAPのチオラクトン誘導体の生合成とその受容体FsrCとの相互作用解析

    山中 陽介、片山 秀和、山村 昭裕、堀田 彰一朗、早川 江、西口 賢三、佐藤 まみ、園元 謙二、中山 二郎、永田 宏次、田之倉 優

    日本農芸化学会  2010年1月 

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    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:東京   国名:日本国  

  • 乳児期の腸内細菌叢偏倚と後のアレルギー発症の関連性に関する前向き調査

    小林 貴子、三坂 祐未、是則 有希、清原 千香子、園元 謙二、中山 二郎

    日本農芸化学会  2010年1月 

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    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:東京   国名:日本国  

  • FLX454次世代シーケンサーを活用した腸内細菌叢解析 招待

    中山二郎

    次世代シーケンス解析 福岡セミナー  2010年1月 

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    記述言語:日本語  

    開催地:福岡   国名:日本国  

  • Development of inhibitors targeting cyclic peptide-mediated quorum sensing of gram-positive bacteria 招待

    Nakayama, J.

    日本農芸化学会2010年度大会  2010年1月 

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    記述言語:日本語  

    開催地:東京  

  • Impact of oral administration of probiotics, Bifidobacterium breve M-16V, in early infancy on the development of gastro-intestinal microbiota 国際会議

    Jiro Nakayama

    2nd TNO beneficial micorobes confernce  2010年3月 

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    記述言語:英語   会議種別:口頭発表(一般)  

  • Aberrancy in faecal bacterial community structures of allergic infants profiled by 16S rRNA pyrosequencing 国際会議

    Jiro Nakayama, Takako Kobayashi, Kenji Sonomoto

    International scientific conference probiotics and prebiotics  2010年6月 

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    記述言語:英語   会議種別:口頭発表(一般)  

  • Development of inhibitors targeting fsr quorum sensing system of Enterococcus faecalis 招待 国際会議

    Jiro Nakayama, Kenji Sonomoto

    3rd ASM Conference on Enterococci  2010年8月 

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    記述言語:英語   会議種別:口頭発表(一般)  

    国名:日本国  

  • 次世代シーケンサーの菌叢解析への応用 招待

    中山二郎

    日本生物工学会  2010年10月 

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    記述言語:日本語   会議種別:公開講演,セミナー,チュートリアル,講習,講義等  

  • 乳児期のビフィダスフローラ形成と宿主の生育・健康状態の関連性解析

    是則有希、小林貴子、鷲尾昌一、清原千香子、園元謙二、中山二郎

    日本生物工学会  2010年10月 

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    記述言語:日本語   会議種別:口頭発表(一般)  

  • Enterococcus faecium NKR-5-3が生産する多成分バクテリオシンの生合成機構の解明

    石橋直樹、姫野康平、益田時光、善藤威史、Wilaipun Pongtep、中山二郎、園元謙二

    日本生物工学会  2010年10月 

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    記述言語:日本語   会議種別:口頭発表(一般)  

  • Antagonist design of gelatinase biosynthesis-activating pheromone of Enterococcus faecalis based on reverse alanine scanning 国際会議

    Ryoji Yokohata, Mami Sato,Kenzo Nishiguchi, Kenji Sonomoto, Jiro Nakayama

    5th International Peptide Symposium  2010年12月 

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    記述言語:英語   会議種別:口頭発表(一般)  

    国名:日本国  

  • リバースアラニンスキャン法による腸球菌のクオラムセンシング阻害剤ZBzl-YAA5911の創製

    横畑綾治、佐藤まみ、西口賢三、園元謙二、鈴木崇、中山二郎

    日本農芸化学会2012年度大会  2011年3月 

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    記述言語:日本語  

    開催地:京都女子大学  

  • リバースアラニンスキャン法に基づいた腸球菌のクオルモン'GBAP'のアンタゴニスト創製

    横畑綾治、佐藤まみ、西口賢三、園元謙二、中山二郎

    日本農芸化学会  2011年3月 

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    記述言語:日本語   会議種別:口頭発表(一般)  

  • 相乗的な抗菌効果を示す2-ペプチドランチビオティック、enterocin Wの構造解析

    澤 稔彦、Pongtep Wilaipun、善藤威史、中山二郎、園元謙二

    日本農芸化学会  2011年3月 

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    記述言語:日本語   会議種別:口頭発表(一般)  

  • Enterococcus faeciumの環状バクテリオシン:初めての発見と構造解析

    井上朋子、姫野康平、冨士田浩二、石橋直樹、善藤威史、中山二郎、Dvid Craik、園元謙二

    日本農芸化学会  2011年3月 

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    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:京都女子大学  

  • 乳幼児の腸内細菌叢と後のアレルギー発症の関連性の解析

    中山二郎、是則有希、清原千香子、鷲尾昌一、園元謙二

    日本農芸化学会  2011年3月 

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    記述言語:日本語   会議種別:口頭発表(一般)  

  • 熟成糠床中の難培養性乳酸菌Lactobacillus acetotoleransの生理的特性

    阪本 直茂、加唐圭太、園元 謙二、中山 二郎

    日本農芸化学会  2011年3月 

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    記述言語:日本語   会議種別:口頭発表(一般)  

  • 16S rRNA pyrosequencing-based investigation of the lactobacilli community in nukadoko, a fermented rice bran used for Japanese pickles 国際会議

    J. Nakayama , N. Sakamoto ,K. Kato , K. Sonomoto

    10th symposium on lactic acid bacteria  2011年8月 

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    記述言語:英語  

    開催地:Egmond Aan Zee   国名:オランダ王国  

  • INVESTIGATION OF THE REGULATION MECHANISM OF MULTIPLE BACTERIOCIN PRODUCTION IN ENTEROCOCCUS FAECIUM NKR-5-3 USING A DEVELOPED ESI-LC/MS SYSTEM 国際会議

    Rodney H Perez, Naoki Ishibashi, Kohei Himeno, Takeshi Zendo, Jiro Nakayama, Kenji Sonomoto

    International Union of Microbiological Societies 2011 Congress  2011年9月 

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    記述言語:英語   会議種別:口頭発表(一般)  

    開催地:札幌  

  • ENHANCED NUKACIN D13E PRODUCTION IN LACTOCOCCUS LACTIS NZ9000 BY THE ADDITIONAL EXPRESSION OF IMMUNITY GENES 招待 国際会議

    Tijo Varghese Puramattathu, Mohammad R. Islam, Mami Nishie, Sae Yanagihara, Jun-Ichi Nagao, Ken-Ichi Okuda, Takeshi Zendo, Jiro Nakayama, Kenji Sonomoto

    International Union of Microbiological Societies 2011 Congress  2011年9月 

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    記述言語:英語   会議種別:口頭発表(一般)  

    開催地:札幌  

  • RING A OF NUKACIN ISK-1: A LIPID II BINDING MOTIF 招待 国際会議

    Mohammad R Islam, Mami Nishie, Akira Takano, Jun-Ichi Nagao, Kouki Shioya, Takeshi Zendo, Jiro Nakayama, Daisuke Kohda, Kenji Sonomoto

    International Union of Microbiological Societies 2011 Congress  2011年9月 

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    記述言語:英語   会議種別:口頭発表(一般)  

    開催地:札幌  

  • SCREENING OF ANTIPATHOGENIC AGENTS TARGETING CYCLIC PEPTIDES-MEDIATED QUORUM SENSING OF GRAM-POSITIVE BACTERIA 招待 国際会議

    Said E Desouky, Yasuhiro Igarashi, Paul Williams, Kenji Sonomoto, Jiro Nakayama

    International Union of Microbiological Societies 2011 Congress  2011年9月 

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    記述言語:英語   会議種別:口頭発表(一般)  

    開催地:札幌  

  • INVESTIGATION OF THE REGULATION MECHANISM OF MULTIPLE BACTERIOCIN PRODUCTION IN ENTEROCOCCUS FAECIUM NKR-5-3 USING A DEVELOPED ESI-LC/MS SYSTEM 招待 国際会議

    Rodney H Perez, Naoki Ishibashi, Kohei Himeno, Takeshi Zendo, Jiro Nakayama, Kenji Sonomoto

    International Union of Microbiological Societies 2011 Congress  2011年9月 

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    記述言語:英語   会議種別:口頭発表(一般)  

    開催地:札幌  

  • ENHANCED NUKACIN D13E PRODUCTION IN LACTOCOCCUS LACTIS NZ9000 BY THE ADDITIONAL EXPRESSION OF IMMUNITY GENES 国際会議

    Tijo Varghese Puramattathu, Mohammad R. Islam, Mami Nishie, Sae Yanagihara, Jun-Ichi Nagao, Ken-Ichi Okuda, Takeshi Zendo, Jiro Nakayama, Kenji Sonomoto

    International Union of Microbiological Societies 2011 Congress  2011年9月 

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    記述言語:英語   会議種別:口頭発表(一般)  

    開催地:札幌  

  • SCREENING OF ANTIPATHOGENIC AGENTS TARGETING CYCLIC PEPTIDES-MEDIATED QUORUM SENSING OF GRAM-POSITIVE BACTERIA 国際会議

    Said E Desouky, Yasuhiro Igarashi, Paul Williams, Kenji Sonomoto, Jiro Nakayama

    International Union of Microbiological Societies 2011 Congress  2011年9月 

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    記述言語:英語   会議種別:口頭発表(一般)  

    開催地:札幌  

  • Community structure of developmental GI tract microbiota in infants and its correlation with allergy development in later life 国際会議

    Jiro Nakayama, Yuki Korenori, Takako Kobayashi, Kenji Sonomoto

    3rd TNO beneficial microbes conference  2012年3月 

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    記述言語:英語  

  • Community structure of developmental GI tract microbiota in infants and its correlation with allergy development in later life 国際会議

    Jiro Nakayama, Yuki Korenori, Takako Kobayashi, Kenji Sonomoto

    3rd TNO beneficial microbes conference  2012年3月 

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    記述言語:英語  

    開催地:Noordwijk   国名:オランダ王国  

  • Asian Microbiome Project: 次世代シーケンサーを用いたアジア人腸内細菌叢のプロファイル化

    東佳那子、百田理恵、山本麻寿紗、林哲也、小椋義俊、黒川顕、豊田敦、加藤和人、AMP study group、園元謙二、渡辺幸一、Lee Yuan Kun、中山二郎

    第21回日本生物工学会九州支部熊本大会  2014年12月 

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    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:熊本・熊本大学工学部   国名:日本国  

  • ウェルシュ菌における内在性クオラムクエンチング誘導物質の精製

    河野通生、大谷郁、Ravindra Pal Singh、石橋直樹、園元謙二、清水徹、中山二郎

    第21回日本生物工学会九州支部熊本大会  2014年12月 

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    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:熊本・熊本大学工学部   国名:日本国  

  • 放線菌の生産する環状デプシペプチドによるクオラムセンシング阻害作用機構の解明

    庄島あかね、松藤貴久、Said E. Desouky、大久保謙一、Ravindra Pal Singh、清水徹、大谷郁、五十嵐康弘、園元謙二、中山二郎

    第21回日本生物工学会九州支部熊本大会  2014年12月 

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    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:熊本・熊本大学工学部   国名:日本国  

  • DDC(Diluted Droplet-Culture)法による腸内細菌叢中の未培養細菌の単離

    木村匠吾,田中 優,中山二郎

    第26回日本生物工学会九州支部長崎大会  2019年12月 

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    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:長崎   国名:日本国  

  • 日本の発酵食品の基盤,麹に含まれるグリコシルセラミドが胆汁酸と腸内細菌の相互作用に及ぼす影響

    永留真優, 北島悠花, 浜島弘史, 柳田晃良, 西向めぐみ, 中村 強, 光武 進, 永尾晃治, 中山二郎, 北垣浩志

    日本農芸化学会2020年度大会  2020年3月 

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    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:福岡   国名:日本国  

  • 糖セラミドの腸内細菌への影響のin vitro解析

    大塚 輝, 戴 凰凰, 永留 真優, 満生 萌水, 田中 猛豪, 中山 二郎, 北垣 浩志

    2021年度日本生物工学会大会  2021年10月 

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    記述言語:日本語   会議種別:口頭発表(一般)  

    国名:日本国  

    Effect of glycosylceramide on the intestinal bacteria in vitro

  • ランチビオティック耐性トランスポーターと基質結合性膜タンパク質による協調的輸送機構

    奥田 賢一、麻生 祐司、中山 二郎、園元 謙二

    日本農芸化学会  2008年3月 

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    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:名古屋・名城大学   国名:日本国  

  • バクテリアにおけるペプチド研究の過去・現在・未来 招待

    中山二郎

    WSフォーラム  2004年1月 

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    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:福岡   国名:日本国  

  • Status of Recent Research in Probiotic used as an additive to livestock. Annual meeting of The Japanese Society for Bioscience, Biotechnology and Agro-Chemistry 招待

    Nitisinprasert S., Hamsupo K., Wanchaitanawong, P., Montet, D., Nakayama, J. and Sonomoto, K

    日本農芸化学会年次大会シンポジウム  2004年3月 

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    記述言語:日本語   会議種別:口頭発表(招待・特別)  

  • アレルギー疾患予防への新展開 招待

    白川太郎,福田早苗,清原千香子,中山二郎,園元謙二

    日本農芸化学会年次大会シンポジウム  2004年3月 

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    記述言語:日本語   会議種別:口頭発表(招待・特別)  

  • 乳幼児における腸内細菌叢とアレルギー(第4報)

    田中 重光,Songjinda, Prapa,久田 貴義,福田 早苗,清原 千香子,白川 太郎,中山 二郎,園元 謙二

    日本農芸化学会  2004年3月 

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    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:広島・広島大学   国名:日本国  

  • 乳幼児における腸内細菌叢とアレルギー-(第3報)-T-RFLPによる腸内フローラの解析

    Prapa, Songjinda, 田中 重光, 黒木 佑美子,久貫 良子,福田 早苗,清原 千香子,白川 太郎,中山 二郎,園元 謙二

    日本農芸化学会  2004年3月 

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    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:広島・広島大学   国名:日本国  

  • Enterococcus faecium NKR-5-3が生産する多成分バクテリオシンの解析

    冨士田 浩二, WILAIPUN, Pongtep, 善藤 威史, LEELAWATCHARAMAS, Vichien, 中山 二郎, 園元 謙二

    日本農芸化学会  2004年3月 

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    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:広島・広島大学   国名:日本国  

  • nukacin ISK-1生合成遺伝子群の異種発現

    麻生 祐司, 古賀 華子, 永尾 潤一, 中山 二郎, 園元 謙二

    日本農芸化学会  2004年3月 

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    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:広島・広島大学   国名:日本国  

  • ランチビオティックnukacin ISK-1の異常アミノ酸形成機構解析

    奥田 賢一, 麻生 祐司, 永尾 潤一, 中山 二郎, 園元 謙二

    日本農芸化学会  2004年3月 

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    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:広島・広島大学   国名:日本国  

  • 第三のナイシン類縁体、ナイシンQ

    米山 史紀, 深尾 匡憲, 帯田 孝之, 善藤 威史, 前仲 勝実, 中山 二郎, 神田 大輔, 園元 謙二

    日本農芸化学会  2004年3月 

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    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:広島・広島大学   国名:日本国  

  • 腸球菌のゼラチナーゼ発現を制御するクォーラムセンシングに関する研究—臨床分離変異株の解析—

    田中 笑美, 狩山 玲子, 公文 裕巳, 中山 二郎, 園元 謙二

    日本農芸化学会  2004年3月 

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    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:広島・広島大学   国名:日本国  

  • 耐塩性乳酸菌Tetragenococcus halophilusが保持する分子シャペロンclpB遺伝子のクローニングおよび機能解析

    吉田 浩之, 杉本真也, TOWSKHONG, Amonlaya, 中山 二郎, 園元 謙二

    日本農芸化学会  2004年3月 

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    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:広島・広島大学   国名:日本国  

  • 腸球菌のゼラチナーゼ発現を,制御するクォーラムセンシングに関する研究−環状ペプチドフェロモンGBAPの生合成機構の解析

    アザブ エッサム, 中山 二郎, 田中 笑美, 西口 賢三, 園元 謙二

    日本農芸化学会  2004年3月 

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    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:広島・広島大学   国名:日本国  

  • 耐塩性乳酸菌Tetragenococcus halophilusのシャペロンの構造と機能

    杉本 真也, トゥスコーウォンアモンラヤ, 中山 二郎, 園元 謙二

    日本農芸化学会  2004年3月 

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    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:広島・広島大学   国名:日本国  

  • Functional analysis of FsrB involved in biosynthesis of cyclic peptide quormone,GBAP, in Enterococcus faecalis

    アザブエッサム, 中山 二郎, 田中 笑美, 園元 謙二

    日本生物工学会  2004年9月 

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    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:名古屋・名城大学   国名:日本国  

    Functional analysis of FsrB involved in biosynthesis of cyclic peptide quormone,GBAP, in Enterococcus faecalis

  • 環状ペプチドによるグラム陽性細菌のクオラムセンシング 招待

    中山二郎

    第38回日本無菌生物ノートバイオロジー学会  2005年1月 

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    記述言語:日本語   会議種別:シンポジウム・ワークショップ パネル(公募)  

  • 乳酸菌の生き残り戦略:バクテリオシン生産およびバイオフィルム形成に関わる−クォーラムセンシング 招待

    中山二郎

    日本農芸化学会年次大会シンポジウム(札幌)  2005年3月 

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    記述言語:日本語  

  • 腸球菌のfsr制御系を標的としたクォーラムセンシング阻害剤開発に関する研究 招待

    中山二郎,田中笑美,西口賢三,陳晟敏,小山望,園元謙二

    2005年度日本生物工学会シンポジウム  2005年11月 

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    記述言語:日本語  

  • 各種分子生物学的手法による乳幼児腸内細菌叢の解析 招待

    中山二郎,Prapa Songjinda,田中重光,立山敦,園元謙二

    第10回腸内細菌学会シンポジウム  2006年1月 

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    記述言語:日本語  

    開催地:東京   国名:日本国  

  • グラム陽性細菌のクォーラムセンシングを標的とした抗感染症剤開発に関する研究 招待

    中山二郎

    第6回21世紀COEミニシンポジウム「産業を創出する応用微生物学」  2006年2月 

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    記述言語:日本語  

    開催地:福岡   国名:日本国  

  • 多様な乳酸菌バクテリオシンの探索とその利用 招待

    善藤威史、中山二郎、園元謙二

    日本農芸化学会  2007年3月 

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    記述言語:日本語   会議種別:シンポジウム・ワークショップ パネル(公募)  

  • New insights into the structure-based functions of lantibiotics 招待

    Asaduzzaman, Sikder、塩屋幸樹、永尾潤一、麻生祐司、中山二郎、園元謙二

    日本農芸化学会  2007年3月 

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    記述言語:日本語   会議種別:シンポジウム・ワークショップ パネル(公募)  

  • 腸球菌のペプチドフェロモンGBAPとその受容体膜タンパク質FsrCの構造解析

    永田 宏次、高橋 美穂子、浅野 敦子、筒井 志穂、山田 邦永、西口 賢三、中山 二郎、園元 謙二、田之倉 優

    日本農芸化学会  2007年3月 

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    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:東京   国名:日本国  

  • 蛍光ラベル化ペプチドを用いたnukacin ISK-1自己耐性タンパク質の機能解析

    奥田 賢一、麻生 祐司、中山 二郎、園元 謙二

    日本農芸化学会  2007年3月 

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    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:東京   国名:日本国  

  • ランチビオティックの異常アミノ酸形成酵素NukMの反応特性解析

    塩屋 幸樹、原田 義孝、永尾 潤一、麻生 祐司、中山 二郎、園元 謙二

    日本農芸化学会  2007年3月 

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    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:東京   国名:日本国  

  • 乳酸菌バクテリオシン、ラクティシンQの生合成メカニズム

    米山 史紀、宮下 紫穂、善藤 威史、中山 二郎、園元 謙二

    日本農芸化学会  2007年3月 

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    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:東京   国名:日本国  

  • コシャペロンDnaJ、GrpEとの協調的機能化を司るグラム陰性細菌由来分子シャペロンDnaKの特異的配列

    鶴野 圭悟、杉本 真也、中山 二郎、園元 謙二

    日本農芸化学会  2007年3月 

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    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:東京   国名:日本国  

  • 耐塩性乳酸菌Tetragenococcus halophilusの保持するコシャペロンGrpEの機能解析

    東 千華、杉本 真也、中山 二郎、園元 謙二

    日本農芸化学会  2007年3月 

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    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:東京   国名:日本国  

  • 球菌の環状ペプチドクォルモン(GBAP)の生合成分子機構に関する研究

    陳 晟敏、中山 二郎、小山 望、西口 賢三、園元 謙二

    日本農芸化学会  2007年3月 

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    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:東京   国名:日本国  

  • 腸球菌の環状ペプチドクォルモン(GBAP)の生合成遺伝子群に関する研究

    小山 望、中山 二郎、陳 晟敏、西口 賢三、園元 謙二

    日本農芸化学会  2007年3月 

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    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:東京   国名:日本国  

  • 分子疫学研究による乳幼児腸内細菌叢とアレルギー罹患の関連性の解析

    中山 二郎、ソンジンダ プラパ、田中 重光、立山 敦、清原 千香子、園元 謙二

    日本農芸化学会  2007年3月 

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    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:東京   国名:日本国  

  • 環状ペプチドを自己誘導因子とするグラム陽性細菌のクォーラムセンシング 招待

    中山二郎

    日本農芸化学会関東支部2007年度第2回支部例会シンポジウム(東京)  2008年1月 

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    記述言語:日本語  

    開催地:東京   国名:日本国  

  • ランチビオティック生合成・分泌に関与するABCトランスポーターの機能解析

    西江 麻美、塩屋 幸樹1、永尾 潤一、中山 二郎、園元 謙二

    日本農芸化学会  2008年3月 

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    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:名古屋・名城大学   国名:日本国  

  • 腸内細菌と健康長寿 アジア人の食と健康と腸内フローラ

    中山 二郎

    機能性食品と薬理栄養  2023年12月  (株)インフォノーツパブリッシング

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    記述言語:日本語  

  • 糠床中の複合微生物叢におけるプロピオン酸共代謝系のメタゲノム解析

    古賀 優梨, 篠田 あかり, 杉浦 俊作, 木村 洋, 中山 二郎

    日本乳酸菌学会誌  2024年6月  日本乳酸菌学会

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    記述言語:日本語  

  • 糖セラミドが腸内細菌に与える影響のin vitro解析

    戴 凰凰, 大塚 輝, 永留 真優, 満生 萌水, 田邊 くるみ, 中山 二郎, 北垣 浩志

    日本栄養・食糧学会大会講演要旨集  2022年5月  (公社)日本栄養・食糧学会

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    記述言語:日本語  

  • 発酵糠床における上下層の菌叢の変化の探索

    杉浦 俊作, 池田 未果, 中村 優一, 森下 美香, 三島 莉子, 中山 二郎

    日本乳酸菌学会誌  2022年6月  日本乳酸菌学会

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    記述言語:日本語  

  • 新奇乳酸菌バクテリオシンの探索および構造・特性の解析

    田中 里緒菜, 庄野 陸太, 野見山 泰成, 中山 二郎, 善藤 威史

    日本生物工学会大会講演要旨集  2023年8月  (公社)日本生物工学会

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    記述言語:日本語  

  • 各種腸内細菌の胆汁酸脱抱合能の比較解析

    中川 大輔, 今西 陸, 中山 二郎

    日本乳酸菌学会誌  2022年6月  日本乳酸菌学会

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    記述言語:日本語  

  • 乳児期早期の粉ミルク摂取が3歳までの牛乳アレルギーのリスク減少と関連する可能性がある エコチル調査

    手塚 純一郎, 碇 航太, 實藤 雅文, 中山 二郎, 西間 大祐, 園田 有里, 小川 昌宣, 下野 昌幸, 菅 礼子, 本荘 哲, 楠原 浩一, 大賀 正一

    日本小児科学会雑誌  2023年2月  (公社)日本小児科学会

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    記述言語:日本語  

  • モンゴル遊牧民の腸内細菌叢と発酵馬乳"アイラグ"の細菌叢に関する研究

    永島 歩武, 篠田 あかり, Tsogtaatar Lhagvajav, Demberel Shirchin, 中山 二郎

    日本乳酸菌学会誌  2023年7月  日本乳酸菌学会

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    記述言語:日本語  

  • モンゴルの発酵馬乳"アイラグ"と遊牧民の糞便のメタゲノム解析 アイラグの乳酸菌と酵母の腸管定着の可能性

    篠田 あかり, 永島 歩武, Lhagvajav Tsogtoaatar, Jamiyan Dugersuren, 森永 由紀, Demberel Shirchin, 中山 二郎

    日本乳酸菌学会誌  2024年6月  日本乳酸菌学会

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    記述言語:日本語  

  • Weissella minor COM株が生産する2種の新奇バクテリオリシンの異種発現系の構築と諸特性の解析

    内藤 温貴, 竹内 愛子, 野見山 泰成, 庄野 陸太, 吉田 遥海, 中山 二郎, 善藤 威史

    日本生物工学会大会講演要旨集  2022年10月  (公社)日本生物工学会

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    記述言語:日本語  

▼全件表示

MISC

  • 腸内マイクロバイオームと糞便及び血清メタボロームの変容は慢性腎臓病の重症度と関連する

    篠田あかり, 中山二郎

    Microbiome Science   2023年10月

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    記述言語:日本語   掲載種別:記事・総説・解説・論説等(学術雑誌)  

  • 変わりゆくアジア食とアジア人の腸内細菌叢 査読

    中山 二郎

    Microbiome Science   2023年2月

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    記述言語:日本語   掲載種別:記事・総説・解説・論説等(学術雑誌)  

  • Crisis of the Asian gut: associations among diet, microbiota, and metabolic diseases 査読

    Phatthanaphong Therdtatha, Akari Shinoda, Jiro Nakayama

    Bioscience of microbiota, food and health   2022年4月

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    記述言語:英語   掲載種別:記事・総説・解説・論説等(学術雑誌)  

    DOI: 10.12938/bmfh.2021-085

  • 腸管機能と腸内細菌の関係を読み解く(2)"胆汁酸"を介した腸内細菌と宿主のクロストーク : 胆汁酸が宿主と腸内細菌の関係を紐解く鍵になる

    田中 優, 中山 二郎

    化学と生物   2022年2月

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    記述言語:日本語   掲載種別:記事・総説・解説・論説等(学術雑誌)  

  • Diversity and dynamics of sourdough lactic acid bacteriota created by a slow food fermentation system 査読

    Mugihito Oshiro, Takeshi Zendo, Jiro Nakayama

    Journal of Bioscience and Bioengineering   2021年4月

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    記述言語:英語   掲載種別:記事・総説・解説・論説等(学術雑誌)  

    Sourdough is a naturally fermented dough that is used worldwide to produce a variety of baked foods. Various lactic acid bacteria (LAB), which can determine the quality of sourdough baked foods by producing metabolites, have been found in the sourdough ecosystem. However, spontaneous fermentation of sourdough leads to unpredictable growth of various micro-organisms, which result in unstable product quality. From an ecological perspective, many researchers have recently studied sourdough LAB diversity, particularly the elucidation of LAB community interactions and the dynamic mechanisms during the fermentation process, in response to requests for the control and design of a desired sourdough microbial community. This article reviews recent advances in the study of sourdough LAB diversity and its dynamics in association with unique characteristics of the fermentation system; it also discusses future perspectives for better understanding of the complex sourdough microbial ecosystem, which can be attained efficiently by both in vitro and in situ experimental approaches

    DOI: https://doi.org/10.1016/j.jbiosc.2020.11.007

  • 【個人差の理解へ向かう肥満症研究 GWAS、エピゲノム、腸内細菌、栄養学的知見から多様な病態を解明し、Precision Medicineをめざす】(第4章)肥満症の個人差 アジア人の食習慣と腸内細菌叢の変化と現代生活習慣病リスク向上の関連性について

    篠田 あかり, 渡邉 麻衣, Therdtatha Phatthanaphong, 中山 二郎

    実験医学   2021年3月

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    記述言語:日本語   掲載種別:記事・総説・解説・論説等(学術雑誌)  

    アジア諸国における肥満や2型糖尿病などの生活習慣病の増加は顕著で深刻である。その背景には食習慣の変化が関係する。一方、数十兆個の細菌が宿るとされる腸内細菌叢が、食と健康のインターフェースとして重要な働きを有し、生活習慣病の増加にも関与していることがわかってきた。われわれは、Asian Microbiome Project(AMP)を設立し、アジアの老若男女の細菌叢を網羅的に調査している。本稿では、そのなかから、各国の食事情を如実に反映していると捉えられる小学児童の調査結果と、成人の肥満と2型糖尿病患者の腸内細菌叢について解説する。(著者抄録)

  • 腸内フローラ研究からみた日本人とアジア人の健康

    中山二郎

    日本食生活学会誌   2018年12月

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    記述言語:日本語   掲載種別:記事・総説・解説・論説等(学術雑誌)  

  • 腸内細菌叢におけるケミカルコミュニケーション

    安達桂香、中山二郎

    腎臓内科・泌尿器科   2018年6月

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    記述言語:日本語   掲載種別:記事・総説・解説・論説等(学術雑誌)  

  • Development of the gut microbiota in infancy and its impact on health in later life

    Masaru Tanaka, Jiro Nakayama

    Allergology International   2017年7月

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    記述言語:英語   掲載種別:記事・総説・解説・論説等(学術雑誌)  

    Gut microbial ecology and function are dynamic in infancy, but are stabilized in childhood. The ‘new friends’ have a great impact on the development of the digestive tract and host immune system. In the first year of life, especially, the gut microbiota dramatically changes through interactions with the developing immune system in the gut. The process of establishing the gut microbiota is affected by various environmental factors, with the potential to be a main determinant of life-long health. In this review, we summarize recent findings regarding gut microbiota establishment, including the importance of various factors related to the development of the immune system and allergic diseases later in life.

  • ヒト腸内細菌叢のダイナミズムとダイバーシティ

    内川彩夏、田中優、中山二郎

    日本乳酸菌学会誌   2017年7月

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    記述言語:日本語   掲載種別:記事・総説・解説・論説等(学術雑誌)  

  • Asian Microbiome Project:アジア人腸内細菌叢の実態調査

    余田美沙子、中山二郎

    バイオサイエンスとバイオインダストリー   2017年6月

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    記述言語:日本語   掲載種別:記事・総説・解説・論説等(学術雑誌)  

  • アジア人の食習慣と腸内細菌叢

    田中優、中山二郎

    Helicobacter Research   2017年4月

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    記述言語:日本語   掲載種別:記事・総説・解説・論説等(学術雑誌)  

  • Quorum quenching strategy targeting Gram-positive pathogenic bacteria

    Jiro Nakayama, Ravindra Pal Singh

    Advances in Microbiology and Infectious Diseases and Public Health   2016年5月

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    記述言語:英語   掲載種別:記事・総説・解説・論説等(学術雑誌)  

  • アジア人の食・健康・腸内細菌叢

    中山 二郎, 本田倫子

    化学療法の領域   2016年2月

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    記述言語:日本語   掲載種別:記事・総説・解説・論説等(学術雑誌)  

  • 進化する次世代シーケンサーによる腸内細菌叢の解析

    東 佳那子, 中山 二郎

    腸内細菌学雑誌   2015年6月

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    記述言語:日本語   掲載種別:記事・総説・解説・論説等(学術雑誌)  

  • 次世代シーケンサーを用いた発酵食品の細菌叢解析ー見えてきた複雑系の深部ー

    小野浩, 中山 二郎

    日本乳酸菌学会誌   2014年3月

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    記述言語:日本語   掲載種別:記事・総説・解説・論説等(学術雑誌)  

  • 腸内細菌と健康:ゆりかごから墓場まで

    中山 二郎

    日本醸造協会   2013年9月

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    記述言語:日本語   掲載種別:記事・総説・解説・論説等(学術雑誌)  

  • 糠床のミクロフローラと乳酸菌の共生

    阪本直茂, 中山二郎

    生物工学会誌   2011年8月

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    記述言語:日本語   掲載種別:記事・総説・解説・論説等(学術雑誌)  

  • 細菌の世界における細胞間ケミカルコミュニケーションとその分子メカニズム

    中山二郎

    腸内細菌学雑誌   2011年4月

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    記述言語:日本語   掲載種別:記事・総説・解説・論説等(学術雑誌)  

  • 赤ちゃんの腸内フローラと過剰衛生仮説

    中山二郎

    科学   2011年3月

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    記述言語:日本語   掲載種別:記事・総説・解説・論説等(学術雑誌)  

  • 乳児期の腸内フローラの偏倚と後のアレルギー疾患発症の関連性について

    中山二郎

    アレルギー・免疫   2010年7月

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    記述言語:日本語   掲載種別:記事・総説・解説・論説等(学術雑誌)  

  • Pyrosequence-based 16S rRNA profiling of gastro-intestinal microbiota

    Jiro Nakayama

    Bioscience & Microflora   2010年4月

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    記述言語:英語   掲載種別:記事・総説・解説・論説等(学術雑誌)  

  • 各種分子生物学的手法による乳幼児腸内細菌叢の解析-幼児アレルギー発症ハイリスク原因究明の大規模疫学調査にむけて-

    中山二郎、田中重光、Prapa Songjinda、立山敦、坪内美樹、清原千香子、白川太郎、園元謙二

    腸内細菌学雑誌   2007年5月

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    記述言語:日本語   掲載種別:記事・総説・解説・論説等(学術雑誌)  

  • ブドウ球菌および腸球菌のクオラムセンシング—環状ペプチドにより制御される病原因子の発現—.

    中山二郎,田中笑美,西口賢三,園元謙二

    臨床と微生物   2004年3月

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    記述言語:日本語   掲載種別:記事・総説・解説・論説等(学術雑誌)  

  • 乳酸菌の世界における細胞間コミュニケーション

    中山二郎、園元謙二

    日本農芸化学会誌   2002年9月

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    記述言語:日本語   掲載種別:記事・総説・解説・論説等(学術雑誌)  

  • Cell to cell communication by autoinducing peptides in gram-positive bacteria

    Sturme, M. H. J., M. Kleerebezem, J. Nakayama, A. D. L. Akkermans, E. E. Vaughan and W. M. de Vos

    Antonie van Leeuwenhoek   2002年1月

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    記述言語:英語   掲載種別:記事・総説・解説・論説等(学術雑誌)  

    DOI: 10.1023/A:1020522919555

  • 日和見感染腸球菌におけるクオーラムセンシング

    中山二郎

    化学と生物   2002年1月

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    記述言語:日本語   掲載種別:記事・総説・解説・論説等(学術雑誌)  

  • グラム陽性細菌のクオーラムセンシング&#8722;バクテリオシンの生産制御と病原因子の発現調節&#8722;

    中山二郎

    日本乳酸菌学会誌   2001年1月

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    記述言語:日本語   掲載種別:記事・総説・解説・論説等(学術雑誌)  

  • ペプチドフェロモンにより制御されるグラム陽性細菌の集団行動

    中山二郎

    蛋白質核酸酵素   2001年1月

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    記述言語:日本語   掲載種別:記事・総説・解説・論説等(学術雑誌)  

  • 細菌の生育を制御する"サイトカイン"様タンパク質

    中山 二郎

    化学と生物   2000年4月

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    記述言語:日本語   掲載種別:記事・総説・解説・論説等(学術雑誌)  

    DOI: 10.1271/kagakutoseibutsu1962.38.247

  • 腸球菌の性フェロモンシグナリングに関する生物有機化学的・分子生物学的研究

    中山二郎

    日本農芸化学会誌   1999年1月

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    記述言語:日本語   掲載種別:記事・総説・解説・論説等(学術雑誌)  

  • 質量分析によるペプチド・タンパク質の構造解析(2)

    中山 二郎, 永田 晋治

    化学と生物   1996年7月

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    記述言語:日本語   掲載種別:記事・総説・解説・論説等(学術雑誌)  

    Mass spectrometry of refractory body composition - 6. Structual analysis of peptide and protein by mass spectrometry (2) .

  • 質量分析によるペプチド・タンパク質の構造解析(1)

    中山 二郎

    化学と生物   1996年6月

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    記述言語:日本語   掲載種別:記事・総説・解説・論説等(学術雑誌)  

    Mass spectrometric analysis of slightly volatile body compositions - 5. Structual analysis of peptides and proteins by mass spectrometric analysis. (1).

  • 細菌の性フェロモン (生物の情報伝達物質<特集>)

    中山 二郎, 鈴木 昭憲

    化学と工業   1990年10月

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    記述言語:日本語   掲載種別:記事・総説・解説・論説等(学術雑誌)  

    Bacterial sex pheromone.

  • 【食・栄養の視点から腸内細菌を探る】変わりゆくアジア食とアジア人の腸内細菌叢

    中山 二郎

    Microbiome Science   2 ( 1 )   6 - 12   2023年1月   ISSN:2758-2094

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    記述言語:日本語   出版者・発行元:(株)先端医学社  

    アジアには各国特有の食文化が根付き,健康効果の観点からも評価されているものが多い.しかし,都市化やグローバリゼーションが進むなかにおいて,アジア人が日々食するものは着々と変化している.われわれは,アジア諸国と共同で,アジア各国民の腸内細菌叢を調査しているが,その食変化がアジア人の腸内細菌叢に大きな変化をもたらしていることを見出している.腸内細菌叢がヒトの健康や疾病と大きくかかわることがつぎつぎと明らかにされている今日において,この事実は全人類が現代社会において直面する局面として目が離せない.本稿では,その変わりゆくアジア人の腸内細菌叢について,筆者らの研究を中心に紹介する.(著者抄録)

  • アジア人における消化管の危機 食事、微生物叢および代謝性疾患の関連性(Crisis of the Asian gut: associations among diet, microbiota, and metabolic diseases)

    Therdtatha Phatthanaphong, Shinoda Akari, Nakayama Jiro

    Bioscience of Microbiota, Food and Health   41 ( 3 )   83 - 93   2022年7月   ISSN:2186-6953

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    記述言語:英語   出版者・発行元:BMFH出版会  

  • 腸管機能と腸内細菌の関係を読み解く(2)"胆汁酸"を介した腸内細菌と宿主のクロストーク : 胆汁酸が宿主と腸内細菌の関係を紐解く鍵になる

    田中 優, 中山 二郎

    化学と生物   60 ( 2 )   79 - 88   2022年2月   ISSN:0453-073X eISSN:1883-6852

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    記述言語:日本語   出版者・発行元:(公社)日本農芸化学会  

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  • 腸管機能と腸内細菌の関係を読み解く "胆汁酸"を介した腸内細菌と宿主のクロストーク 胆汁酸が宿主と腸内細菌の関係を紐解く鍵になる

    田中 優, 中山 二郎

    化学と生物   60 ( 2 )   79 - 88   2022年2月   ISSN:0453-073X

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    記述言語:日本語   出版者・発行元:(公社)日本農芸化学会  

  • C型肝炎ウイルス感染によるDysbiosis(腸内フローラ異常)と病態進展へのインパクト

    井上 貴子, 中山 二郎, 田中 靖人

    Nagoya Medical Journal   2020年12月

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    記述言語:日本語   掲載種別:記事・総説・解説・論説等(学術雑誌)  

    病期の異なるC型慢性肝炎(CHC)患者と健常人の腸内フローラを比較することで、HCV感染と腸内フローラの変化・病態進展との関連について検討した。CHC患者166例と健常人23例を対象とした。CHC患者では、健常人の腸内フローラ主構成菌種であるラクノスピラ科やルミノコッカス科が減少し、レンサ球菌科やラクトバシラス科、腸内細菌科の細菌が増加していた。HCV感染者では病初期の段階から腸内フローラが変化し、病期の進行につれてdysbiosisが進行することが証明された。便中アンモニア産生菌の量に比例して便pHが上昇していることから、dysbiosisが高アンモニア血症の原因となる可能性を間接的に示すことができた。

  • 麹由来グリコシルセラミドによるコレステロール代謝改善

    北垣 浩志, 浜島 弘史, 中山 二郎, 永尾 晃治

    バイオサイエンスとインダストリー   2020年9月

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    記述言語:日本語   掲載種別:記事・総説・解説・論説等(学術雑誌)  

  • セファゾリンとナイシンAの乳房炎原因菌に対する相乗的殺菌効果

    北崎 宏平, 古賀 祥子, 桑野 剛一, 善藤 威史, 園元 謙二, 片本 宏, 永利 浩平, 中山 二郎

    日本獣医師会雑誌   2019年11月

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    記述言語:日本語   掲載種別:記事・総説・解説・論説等(学術雑誌)  

  • 【発酵食品の機能性と関連領域】麹に含まれるグリコシルセラミドの健康効果 査読

    山本 裕貴, 浜島 弘史, 田中 優, 藤丸 裕貴, 阪本 真由子, 宮川 幸, 西向 めぐみ, 柳田 晃良, 中村 強, 光武 進, 永尾 晃治, 中山 二郎, 北垣 浩志

    生物工学会誌   2019年4月

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    記述言語:日本語   掲載種別:記事・総説・解説・論説等(学術雑誌)  

    麹に含まれるグリコシルセラミドの健康効果

  • 【アレルギー疾患とマイクロバイオーム】ヒト常在マイクロバイオーム研究の技術の進歩

    田中 優, 中山 二郎

    アレルギーの臨床   2019年2月

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    記述言語:日本語   掲載種別:記事・総説・解説・論説等(学術雑誌)  

    近年の次世代シークエンサー、質量分析計、バイオインフォマティクスの著しい技術進歩により、腸内フローラなどの非常に複雑なヒト常在マイクロバイオームの研究が加速している。これらのオミックス研究分野の技術革新により、マイクロバイオームを構成する微生物群の分類学的および機能的特徴やバイオマーカーの探索のような細部にわたるデータ収集から俯瞰的な考察までが可能となった。そして得られた知見を実証する動物実験の技術も成熟してきている。マイクロバイオームのコミュニティーをインビトロに再現し、その組成や機能変化をリアルタイムでモニタリングするヒト腸管モデルもまた注目される。(著者抄録)

  • Asian gut microbiome

    Jiro Nakayama, Heping Zhang, Yuan Kun Lee

    Science Bulletin   2017年6月

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    記述言語:英語   掲載種別:記事・総説・解説・論説等(学術雑誌)  

    DOI: 10.1016/j.scib.2017.04.001

  • 16S rRNA Metagenomics of Asian Gut Microbiota

    Juma Kisuse, Jiro Nakayama

    Understanding Host-Microbiome Interactions - An Omics Approach   2017年1月

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    記述言語:英語   掲載種別:記事・総説・解説・論説等(学術雑誌)  

    DOI: 10.1007/978-981-10-5050-3

  • グローバル化する腸内フローラ研究とAsian Microbiome Project

    東 佳那子, 中山 二郎

    ソフト・どリング技術資料   2015年5月

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    記述言語:日本語   掲載種別:記事・総説・解説・論説等(学術雑誌)  

  • 次世代シークェンサーによるヒト腸内フローラの解析

    中山 二郎

    九州大学中央分析センター報告   2012年12月

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    記述言語:日本語   掲載種別:記事・総説・解説・論説等(学術雑誌)  

  • 16S rRNA遺伝子の大量シークェンシングによる菌叢解析の現状と問題点、日本乳酸菌学会誌、Vol.23, pp.24-33(2012).

    是則有希、Jiahui Jiang、中山二郎

    日本乳酸菌学会誌   2012年3月

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    記述言語:日本語   掲載種別:記事・総説・解説・論説等(学術雑誌)  

  • グラム陽性細菌のクオラムセンシング研究の最前線

    佐藤まみ、中山二郎

    日本乳酸菌学会誌   2010年4月

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    記述言語:日本語   掲載種別:記事・総説・解説・論説等(学術雑誌)  

  • 性的領域への関心が低い男性人格に関する検討

    辻村晃, 山本圭介, 福原慎一郎, 平井利明, 植田知博, 中山二郎, 木内寛, 高尾徹也, 宮川康, 奥山明彦, 松下正輝

    日本泌尿器科学会雑誌   2009年2月

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    記述言語:日本語  

    性的領域への関心が低い男性人格に関する検討

  • AVSS時にヒト男性の人格が影響をおよぼす視覚関心領域に関する検討

    辻村晃, 山本圭介, 福原慎一郎, 平井利明, 植田知博, 中山二郎, 木内寛, 高尾徹也, 宮川康, 松下正輝, 奥山明彦

    日本性機能学会雑誌   2008年8月

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    記述言語:日本語  

    AVSS時にヒト男性の人格が影響をおよぼす視覚関心領域に関する検討

  • 視線追跡装置と人格評価による性的関心度に対する性差解析

    辻村晃, 山本圭介, 福原慎一郎, 平井利明, 植田知博, 中山二郎, 木内寛, 小森和彦, 松岡庸洋, 高尾徹也, 宮川康, 奥山明彦, 松下正輝

    日本泌尿器科学会雑誌   2008年2月

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    記述言語:日本語  

    視線追跡装置と人格評価による性的関心度に対する性差解析

  • Lantibiotic engineering Molecular characterization and exploitation of lantibiotic-synthesizing enzymes for peptide engineering

    Jun Ichi Nagao, Yuji Aso, Kouki Shioya, Jiro Nakayama, Kenji Sonomoto

    Journal of Molecular Microbiology and Biotechnology   2007年9月

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    記述言語:英語   掲載種別:記事・総説・解説・論説等(学術雑誌)  

    Lanthionine-containing peptide antibiotics called lantibiotics are produced by a large number of Gram-positive bacteria. Nukacin ISK-1 produced by Staphylococcus warneri ISK-1 is type-A(II) lantibiotic. Ribosomally synthesized nukacin ISK-1 prepeptide (NukA) consists of an N-terminal leader peptide followed by a C-terminal propeptide moiety that undergoes several post-translational modification events including unusual amino acid formation by the modification enzyme NukM, cleavage of leader peptide and export by the dual functional ABC transporter NukT, finally yielding a biologically active peptide. Unusual amino acids in lantibiotics contribute to biological activity and also structural stability against proteases. Thus, lantibiotic-synthesizing enzymes have a high potentiality for peptide engineering by introduction of unusual amino acids into desired peptides with altering biological and physicochemical properties, e.g., activity and stability, termed lantibiotic engineering. We report the establishment of a heterologous expression of nukacin ISK-1 biosynthetic gene cluster by the nisin-controlled expression system and discuss our recent progress in understanding of the biosynthetic enzymes for nukacin ISK-1 such as localization, molecular interaction in biophysical and biochemical aspects. Substrate specificity of the lantibiotic-synthesizing enzymes was evaluated by complementation of the biosynthetic enzymes (LctM and LctT) of closely related lantibiotic lacticin 481 for nukacin ISK-1 biosynthesis. We further explored a rapid and powerful tool for introduction of unusual amino acids by co-expression of hexa-histidine-tagged NukA and NukM in Escherichia coli.

    DOI: 10.1159/000104749

  • 視線追跡装置を用いたヒト男性の性的興味とMMPIによる人格評価との関連性

    辻村晃, 福原慎一郎, 平井利明, 植田知博, 中山二郎, 木内寛, 小森和彦, 松岡庸洋, 高尾徹也, 宮川康, 高田晋吾, 奥山明彦, 松下正輝

    日本性機能学会雑誌   2007年8月

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    記述言語:日本語  

    視線追跡装置を用いたヒト男性の性的興味とMMPIによる人格評価との関連性

  • Lantibiotics Insight and foresight for new paradigm

    Jun ichi Nagao, Sikder M. Asaduzzaman, Yuji Aso, Ken ichi Okuda, Jiro Nakayama, Kenji Sonomoto

    Journal of Bioscience and Bioengineering   2006年9月

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    記述言語:英語   掲載種別:記事・総説・解説・論説等(学術雑誌)  

    Lantibiotics are a unique type of antimicrobial peptide produced by a large number of gram-positive bacteria that contain unusual amino acids, such as lanthionine and dehydrated amino acids. Ribosomally synthesized lantibiotic prepeptide consists of an N-terminal leader peptide followed by a C-terminal propeptide moiety that undergoes several post-translational modification events to yield a biologically active lantibiotic. Research on lantibiotics has drawn much attention in recent years and has undergone extensive progress as a step forward to the next paradigm. Unusual amino acids in lantibiotics solely contribute to their biological activity and also enhance their structural stability. Thus, enzymes involved in lantibiotic biosynthesis would have a high potential for peptide engineering by introducing unusual amino acids into desired peptides, which may establish a universal approach to advance the structural design of novel peptides, termed lantibiotic engineering. In this review, we focus on recent development with contemporary innovations and perspective of lantibiotic research.

    DOI: 10.1263/jbb.102.139

  • 抗菌性ペプチドであるバクテリオシンとその耐性メカニズム

    奥田 賢一, 麻生 祐司, 中山 二郎

    ニューフードインダストリー   2006年3月

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    記述言語:日本語  

  • Identification and production of a bacteriocin from Enterococcus mundtii QU2 isolated from soybean

    善藤威史, Eungruttanagorn, N, 藤岡澄江, 田代幸寛, 野村久美, 世良芳子, 小林元太, 中山二郎, 石崎文彬, 園元謙二

    Journal of Applied Microbiology   2005年11月

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    記述言語:その他  

    Aims: Identification of the bacteriocin produced by Enterococcus mundtii QU 2 newly isolated from soybean and fermentative production of the bacteriocin. Methods and Results: The bacteriocin produced by Ent. mundtii QU 2 inhibited the growth of various indicator strains, including Enterococcus, Lactobacillus, Leuconostoc, Pediococcus and Listeria. The bacteriocin activity was stable at wide pH range and against heat treatment, but completely abolished by proteolytic enzymes. The bacteriocin was purified from the culture supernatant by the three-step chromatographic procedure. Mass spectrometry, amino acid sequencing and DNA sequencing revealed that the bacteriocin was similar to class IIa bacteriocins produced by other Ent. mundtii strains. The bacteriocin production decreased in the absence of glucose, nitrogen sources, or Tween 80 in MRS medium. Additionally, it was strongly suppressed by addition of Ca2+(CaCO3or CaCl2). In pH-controlled fermentations, the highest bacteriocin production was achieved at pH 6.0 whereas the highest cell growth was obtained at pH 7.0. Conclusions: Ent. mundtii QU 2 produced a class IIa bacteriocin. Some growth factors (e.g. Ca2+and pH) influenced the bacteriocin production. Significance and Impact of the Study: A new soybean isolate, Ent. mundtii QU 2 was found to be a class IIa bacteriocin producer. Factors influencing the bacteriocin production described herein are valuable for applications of the bacteriocins from Ent. mundtii strains. © 2005 The Society for Applied Microbiology.

    DOI: 10.1111/j.1365-2672.2005.02704.x

  • Lanthionine introduction into nukacin ISK-1 prepeptide by co-expression. with modification enzyme NAM in Escherichia coli

    J Nagao, Y Harada, K Shloya, Y Aso, T Zendo, J Nakayama, K Sonomoto

    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS   2005年10月

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    記述言語:英語  

    We demonstrated lanthionine introduction into hexa-histidine-tagged (His-tagged) nukacin ISK-1 prepeptide NukA by modification enzyme NukM in Escherichia coli. Co-expression of nukA and nukM, purification of the resulting His-tagged prepeptide by affinity chromatography, and subsequent mass spectrometry analysis showed that the prepeptide was converted into a postulated peptide with decrease in mass of 72 Da which resulted from dehydration of four amino acids. Characterization of the resultant prepeptide indicated the presence of unusual amino acids, such as dehydrated amino acid, lanthionine or 3-methyllanthionine, in its C-terminal propeptide moiety. The modified prepeptide encompassing the leader peptide attached to the post-translation ally modified propeptide moiety was readily obtained by one-step purification. Our findings will thus be a powerful tool for introducing unusual amino acids aimed at peptide engineering and also helpful to provide new insight for further understanding of lanthionine-forming enzymes for lantibiotics. (c) 2005 Elsevier Inc. All rights reserved.

    DOI: 10.1016/j.bbrc.2005.08.125

  • An agr-like two-component regulatory system in Lactobacillus plantarum is involved in production of a novel cyclic peptide and regulation of adherence

    MHJ Sturme, J Nakayama, D Molenaar, Y Murakami, R Kunugi, T Fujii, EE Vaughan, M Kleerebezem, WM de Vos

    JOURNAL OF BACTERIOLOGY   2005年8月

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    記述言語:英語  

    We have analyzed a locus on the annotated Lactobacillus plantarum WCFS1 genome that showed homology to the staphylococcal agr quorum-sensing system and designated it lam for Lactobacillus agr-like module. Production of the lamBDCA transcript was shown to be growth phase dependent. Analysis of a response regulator-defective mutant (Delta lamA) in an adherence assay showed that lam regulates adherence of L. plantarum to a glass surface. Global transcription analysis of the wild-type and Delta lamA strains in early, mid-, and late log phase of growth was performed using a clone-based microarray. Remarkably, only a small set of genes showed significant differences in transcription profiles between the wild-type and lamA mutant strains. The microarray analysis confirmed that lamBDCA is autoregulatory and showed that lamA is involved in regulation of expression of genes encoding surface polysaccharides, cell membrane proteins, and sugar utilization proteins. The lamBD genes encoding the putative autoinducing peptide precursor (LamD) and its processing protein (LamB) were overexpressed using the nisin-controlled expression system, and culture supernatants were analyzed by liquid chromatography/mass spectrometry (LC/MS) to identify overproduced Lam-derived peptides. In this way, a cyclic thiolactone pentapeptide that possesses a ring structure similar to those of autoinducing peptides of the staphylococcal agr system was identified. The peptide was designated LamD558, and its sequence (CVGIW) matched the annotated precursor peptide sequence. Time course analysis of wild-type culture supernatants by LC/MS indicated that LamD558 production was increased markedly from mid-log to late log growth phase. This is the first example of an agr-like system in nonpathogenic bacteria that encodes a cyclic thiolactone autoinducing peptide and is involved in regulation of adherence.

    DOI: 10.1128/JB.187.15.5224-5235.2005

  • 乳酸菌の機能制御とクォーラムセンシング

    中山二郎,善藤威史,園元謙二

    バイオサイエンスインダストリー   2004年10月

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    記述言語:日本語   掲載種別:記事・総説・解説・論説等(学術雑誌)  

  • Characterization of a gene cluster of Staphylococcus warneri ISK-1 encoding the biosynthesis of and immunity to the lantibiotic, nukacin ISK-1

    Y Aso, T Sashihara, J Nagao, Y Kanemasa, H Koga, T Hashimoto, T Higuchi, A Adachi, H Nomiyama, A Ishizaki, J Nakayama, K Sonomoto

    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY   2004年8月

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    記述言語:英語  

    We characterized a gene cluster in a plasmid designated pPI-1 of Staphylococcus warneri ISK-1 encoding the biosynthesis of and immunity to the lacticin-481 type lantibiotic, nukacin ISK-1. The DNA sequence suggested that the nukacin ISK-1 gene cluster consists of at least six genes, nukA (a structural gene), -M, -T, -F, -E, -G, and two open reading frames, ORF1 and ORF7. NukM and NukT were predicted to be involved in post-translational modification and secretion of nukacin ISK-1 respectively. NukF, -E, and -G were predicted to form a membrane complex which contributes to self-protection from nukacin ISK-1. Transcriptional analyses revealed that nukM through ORF7 comprises an operon, and that ORF1 is transcribed independently from downstream of nukA. The transcriptional levels of the nukA and nukM genes were enhanced by osmotic stress. The expression level of the nukA transcript was scarcely enhanced by nukacin ISK-1, suggesting that expression is not under the control of the autoregulatory circuit.

    DOI: 10.1271/bbb.68.1663

  • 腸内フローラの構造解析 : DGGE/TGGE法による腸内細菌叢解析

    中山 二郎, 福井 学, SONJINDA Prapa, 田中 重光, 久貫 良子, 園元 謙二

    腸内細菌学雑誌 = Journal of intestinal microbiology   2004年7月

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    記述言語:日本語  

    Analysis of the Composition of the Intestinal Flora : Analysis of Intestinal Bacterial Community by DGGE/TGGE

  • 乳酸菌バクテリオシンとその応用

    冨士田浩二,善藤威史,中山二郎,園元謙二

    防菌防黴誌   2004年3月

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    記述言語:日本語   掲載種別:記事・総説・解説・論説等(学術雑誌)  

  • 新しい乳酸菌バクテリオシンの探索と利用

    善藤威史,中山二郎,園元謙二

    バイオサイエンスとインダストリー   2003年9月

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    記述言語:日本語   掲載種別:記事・総説・解説・論説等(学術雑誌)  

  • Effect of heterologous expression of molecular chaperone DnaK from Tetragenococcus halophilus on salinity adaptation of Escherichia coli

    S Sugimoto, J Nakayama, D Fukuda, S Sonezaki, M Watanabe, A Tosukhowong, K Sonomoto

    JOURNAL OF BIOSCIENCE AND BIOENGINEERING   2003年8月

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    記述言語:英語  

    Molecular chaperone DnaK of halophilic Tetragenococcus halophilus JCM5888 was characterized under salinity conditions both in vitro and in vivo. The dnaK gene was cloned into an expression vector And transformed into Escherichia coli. The DnaK protein obtained from the recombinant E. coli showed a significantly higher refolding activity of denatured lactate dehydrogenase than that from non-halophilic Lactococcus lactis under NaCl concentrations higher than I M. E. coli without the overexpression of DnaK exhibited a growth profile with a prolonged lag phase and suppressed maximum cell density in Luria-Bertani medium containing 5% (0.86 M) NaCl. On the contrary, the overexpression of T halophilus DnaK greatly shortened this prolonged lag phase with no effect on maximum growth, while that of L. lactis DnaK decreased maximum growth. The amount of protein aggregates was increased by salt stress in the E. coli cells, while this aggregation was greatly suppressed by the overexpression of T halophilus DnaK. These results suggest that heterologous overexpression of T halophilus DnaK, via its chaperone activity, promotes salinity adaptation of E. coli.

  • High-throughput PCR screening of genes for three-component regulatory system putatively involved in quorum sensing from low-G + C Gram-positive bacteria

    J Nakayama, ADL Akkermans, WM De Vos

    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY   2003年3月

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    記述言語:英語  

    Quorum sensing of Gram-positive bacteria is often regulated by three-component regulatory system composed of autoinducing peptide, sensor kinase and response regulator. We used PCR to study a gene cassette encoding this three-component regulatory system. Degenerate primers were designed from consensus amino acid sequences in the HPK10 subfamily, mostly involved in quorum sensing. Products amplified from genomic DNA of Lactobacillus, Enterococcus, and Clostridium species were cloned and sequenced; their deduced amino acid sequences were similar to those of members of the HPK10 subfamily. Complete genes for the putative gene cassette were cloned by inverse PCR from L. paracasei E93490 and L. plantarum WCFS6. Phylogenetic analysis grouped the cloned putative HPKs into the HPK10 subfamily. These results indicated the usefulness of this high-throughput gene screening and suggested that the three-component regulatory gene cassette are widely present.

    DOI: 10.1271/bbb.67.480

  • 分子進化型生理活性ペプチドランチビオティック

    永尾潤一,麻生 祐司,中山 二郎,園元 謙二

    日本生物工学会   2003年1月

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    記述言語:日本語   掲載種別:記事・総説・解説・論説等(学術雑誌)  

  • 乳酸菌の多様な新機能を求めて

    麻生 祐司, 永尾 潤一, 中山 二郎, 園元 謙二

    日本農芸化学会誌   2003年1月

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    記述言語:日本語   掲載種別:記事・総説・解説・論説等(学術雑誌)  

    DOI: 10.1271/nogeikagaku1924.77.741

  • Functional analysis of TraA, the sex pheromone receptor encoded by pPD1, in a promoter region essential for the mating response in Enterococcus faecalisi

    T Horii, H Nagasawa, J Nakayama

    JOURNAL OF BACTERIOLOGY   2002年11月

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    記述言語:英語  

    Conjugative transfer of a bacteriocin plasmid, pPD1, of Enterococcus faecalis is induced in response to a peptide sex pheromone, cPD1, secreted from plasmid-free recipient cells. cPD1 is taken up by a pPD1 donor cell and binds to an intracellular receptor, TraA. Once a recipient cell acquires pPD1, it starts to produce an inhibitor of cPD1, termed iPD1, which functions as a TraA antagonist and blocks self-induction in donor cells. In this study, we discuss how TraA transduces the signal of cPD1 to the mating response. Gel mobility shift assays indicated that TraA is bound to a traA-ipd intergenic region, which is essential for cPD1 response. DNase I footprinting analysis suggested the presence of one strong (tab1) and two weak (tab2 and tab3) TraA-binding sites in the intergenic region. Primer extension analysis implied that the transcriptional initiation sites of traA and ipd were located in the intergenic region. Northern analysis showed that cPD1 upregulated and downregulated transcription of ipd and traA, respectively. The circular permutation assay showed that TraA bent a DNA fragment corresponding to the tab1 region, and its angle was changed in the presence of cPD1 or iPD1. From these data, we propose a model that TraA changes the conformation of the tab1 region in response to cPD1 and upregulates the transcription of ipd, which may lead to expression of genes required for the mating response.

    DOI: 10.1128/JB.22.6343-6350.2002

  • 細菌の環状ペプチドフェロモン

    中山二郎、園元謙二

    酵素工学ニュース   2002年1月

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    記述言語:日本語   掲載種別:記事・総説・解説・論説等(学術雑誌)  

  • Blasticidin A as an Inhibitor of Aflatoxin Production by Aspergillus parasiticus

    SAKUDA Shohei, ONO Makoto, IKEDA Hiroyuki, NAKAMURA Takefumi, INAGAKI Yasuhito, KAWACHI Ryu, NAKAYAMA Jiro, SUZUKI Akinori, ISOGAI Akira, NAGASAWA Hiromichi

    Journal of antibiotics   2000年11月

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    記述言語:英語   掲載種別:記事・総説・解説・論説等(学術雑誌)  

    Blasticidin A as an inhibitor of aflatoxin production by Aspergillus parasiticus(共著)

    DOI: 10.7164/antibiotics.53.1265

▼全件表示

Works(作品等)

  • 目で見る微生物学(DVD)

    中山二郎

    2011年12月

  • 目で見る微生物学(DVD)

    中山二郎

    2011年12月

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  • 地域集団でのコホート研究による便中細菌診断妥当性の研究

    2004年

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  • リウマチアレルギー疾患の早期診断に関する研究

    2002年

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産業財産権

特許権   出願件数: 3件   登録件数: 2件
実用新案権   出願件数: 0件   登録件数: 0件
意匠権   出願件数: 0件   登録件数: 0件
商標権   出願件数: 0件   登録件数: 0件

所属学協会

  • 日本乳酸菌学会

  • 日本生物工学会

  • 日本農芸化学会

  • 日本腸内細菌学会

  • 日本食品科学工学会

  • 腸内細菌学会

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  • 日本食品科学工学会

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  • 日本農芸化学会

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  • 日本腸内細菌学会

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  • 日本生物工学会

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  • 日本分子生物学会

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  • 日本乳酸菌学会

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委員歴

  • 日本腸内細菌学会   評議員   国内

    2023年6月 - 2024年6月   

  • 日本農芸化学会理事会   日本農芸化学会理事  

    2021年5月 - 現在   

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    団体区分:学協会

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  • 日本農芸化学会西日本支部   日本農芸化学会西日本支部長  

    2021年5月 - 現在   

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    団体区分:学協会

    researchmap

  • 日本農芸化学会西日本支部   会長   国内

    2021年5月 - 2023年5月   

  • 日本農芸化学会   理事   国内

    2021年5月 - 2023年5月   

  • 日本農芸化学会西日本支部   日本農芸化学会西日本支部長   国内

    2021年5月 - 2023年5月   

  • 日本農芸化学会   西日本支部長   国内

    2021年5月 - 2023年5月   

  • 日本農芸化学会   産学官学術交流委員   国内

    2019年4月 - 2021年3月   

  • 日本農芸化学会   学術活動強化委員   国内

    2018年4月 - 2021年3月   

  • 日本農芸化学会西日本支部   幹事   国内

    2015年4月 - 2017年3月   

  • 日本農芸化学会西日本支部   庶務幹事   国内

    2015年4月 - 2017年3月   

  • 九州大学   研究教育支援センター運営委員会  

    2013年4月 - 現在   

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    団体区分:その他

    福センター長

    researchmap

  • 日本農芸化学会   参与   国内

    2012年4月 - 2021年3月   

  • 日本生物工学会九州支部   理事   国内

    2009年4月 - 2010年3月   

  • 日本乳酸菌学会   理事   国内

    2007年4月 - 2011年3月   

  • 日本乳酸菌学会   評議員   国内

    2003年11月 - 2007年5月   

  • 日本農芸化学会西日本支部   日本農芸化学会西日本支部参与   国内

    2002年5月 - 2025年5月   

▼全件表示

学術貢献活動

  • 座長(Chairmanship) 国際学術貢献

    10th TNO Beneficial Microbes Conference  ( アムステルダム ) 2023年11月 - 2024年11月

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    種別:大会・シンポジウム等 

    参加者数:300

  • 座長(Chairmanship)

    日本農芸化学会2022年度広島大会  ( 広島(リモート) ) 2023年3月 - 2021年3月

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    種別:大会・シンポジウム等 

    参加者数:1,600

  • 学術論文等の審査

    役割:査読

    2023年

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    種別:査読等 

    外国語雑誌 査読論文数:10

    日本語雑誌 査読論文数:0

    国際会議録 査読論文数:0

    国内会議録 査読論文数:0

  • 座長(Chairmanship) 国際学術貢献

    9th TNO Beneficial Microbes Conference  ( アムステルダム ) 2022年11月

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    種別:大会・シンポジウム等 

    参加者数:300

  • Microbiome Science

    2022年9月 - 現在

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    種別:学会・研究会等 

  • Frontiers in Microbiology 国際学術貢献

    2022年9月 - 現在

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    種別:学会・研究会等 

  • 世話人代表

    Visionary100農芸化学シンポジウム:健康長寿社会に向けての腸内細菌科学の新展開  ( リモート ) 2021年9月

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    種別:大会・シンポジウム等 

    参加者数:200

  • 座長(Chairmanship) 国際学術貢献

    8th TNO Beneficial Microbes Conference  ( Remote Holland ) 2021年5月

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    種別:大会・シンポジウム等 

    参加者数:300

  • 座長(Chairmanship)

    日本農芸化学会2021年度仙台大会  ( 仙台(リモート) ) 2021年3月

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    種別:大会・シンポジウム等 

    参加者数:1,600

  • 学術論文等の審査

    役割:査読

    2021年

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    種別:査読等 

    外国語雑誌 査読論文数:14

    日本語雑誌 査読論文数:0

    国際会議録 査読論文数:0

    国内会議録 査読論文数:0

  • 大会実行委員

    日本農芸化学会2020年度福岡大会  ( 九州大学伊都キャンパス(福岡市) ) 2020年3月

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    種別:大会・シンポジウム等 

  • 学術論文等の審査

    役割:査読

    2020年

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    種別:査読等 

    外国語雑誌 査読論文数:16

    日本語雑誌 査読論文数:2

    国際会議録 査読論文数:0

    国内会議録 査読論文数:0

  • 学術論文等の審査

    役割:査読

    2019年

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    種別:査読等 

    外国語雑誌 査読論文数:16

    日本語雑誌 査読論文数:2

    国際会議録 査読論文数:0

    国内会議録 査読論文数:0

  • 座長(Chairmanship) 国際学術貢献

    7th TNO Beneficial Microbes Conference  ( Amsterdam Holland ) 2018年11月

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    種別:大会・シンポジウム等 

    参加者数:300

  • 座長(Chairmanship)

    日本農芸化学会2017年度大会  ( 名城大学 ) 2018年3月 - 2016年3月

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    種別:大会・シンポジウム等 

    参加者数:3,000

  • 学術論文等の審査

    役割:査読

    2018年

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    種別:査読等 

    外国語雑誌 査読論文数:20

    日本語雑誌 査読論文数:6

    国際会議録 査読論文数:0

    国内会議録 査読論文数:0

  • 座長(Chairmanship) 国際学術貢献

    6th TNO Beneficial Microbes Conference  ( Amsterdam Holland ) 2017年10月

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    種別:大会・シンポジウム等 

    参加者数:300

  • 実行委員

    日本乳酸菌学会年大会  ( 福岡 ) 2017年7月

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    種別:大会・シンポジウム等 

    参加者数:200

  • 学術論文等の審査

    役割:査読

    2017年

     詳細を見る

    種別:査読等 

    外国語雑誌 査読論文数:20

    日本語雑誌 査読論文数:3

    国際会議録 査読論文数:0

    国内会議録 査読論文数:0

  • 座長(Chairmanship) 国際学術貢献

    5th TNO Beneficial Microbes Conference  ( Amsterdam Holland ) 2016年10月

     詳細を見る

    種別:大会・シンポジウム等 

  • アドバイザリー 国際学術貢献

    5th TNO Beneficial Microbes Conference  ( Amsterdam Holland ) 2016年10月

     詳細を見る

    種別:大会・シンポジウム等 

    参加者数:200

  • 座長(Chairmanship)

    Visonary農芸化学100シンポジウム  ( 京都大学益川ホール ) 2016年10月

     詳細を見る

    種別:大会・シンポジウム等 

  • オーガナイザー

    日本農芸化学会創立100周年に向けたシンポジウム  ( 京都大学益川ホール ) 2016年10月

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    種別:大会・シンポジウム等 

    参加者数:100

  • 座長(Chairmanship)

    第68回日本生物工学会  ( 富山国際会議場 ) 2016年9月

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    種別:大会・シンポジウム等 

  • オーガナイザー

    第68回日本生物工学会  ( 富山国際会議場 ) 2016年9月

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    種別:大会・シンポジウム等 

    参加者数:100

  • 座長(Chairmanship)

    日本農芸化学会2016年度大会  ( 京都女子大 ) 2016年3月

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    種別:大会・シンポジウム等 

  • オーガナイザー 国際学術貢献

    第67回日本生物工学会  ( 鹿児島 ) 2015年10月

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    種別:大会・シンポジウム等 

    参加者数:100

  • 座長(Chairmanship) 国際学術貢献

    第67回日本生物工学会  ( 鹿児島 ) 2015年10月

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    種別:大会・シンポジウム等 

  • 日本農芸化学会誌(化学と生物)

    2015年4月 - 2019年3月

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    種別:学会・研究会等 

  • 座長(Chairmanship)

    日本農芸化学会2015年度大会  ( 岡山 ) 2015年3月

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    種別:大会・シンポジウム等 

  • オーガナイザー

    第88回日本細菌学会シンポジウム  ( Hague Holland ) 2015年3月

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    種別:大会・シンポジウム等 

    参加者数:150

  • 座長(Chairmanship) 国際学術貢献

    4th TNO Beneficial Microbes Conference  ( Hague Holland ) 2015年3月

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    種別:大会・シンポジウム等 

  • アドバイザリー 国際学術貢献

    4th TNO Beneficial Microbes Conference  ( Hague Holland ) 2015年3月

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    種別:大会・シンポジウム等 

    参加者数:150

  • 座長(Chairmanship)

    日本農芸化学会2014年度大会  ( 東京 ) 2014年3月

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    種別:大会・シンポジウム等 

  • 座長(Chairmanship) 国際学術貢献

    3rd TNO Beneficial Microbes Conference  ( Noordwijk Holland ) 2012年3月

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    種別:大会・シンポジウム等 

  • アドバイザリー 国際学術貢献

    3rd TNO Beneficial Microbes Conference  ( Noordwijk Holland ) 2012年3月

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    種別:大会・シンポジウム等 

    参加者数:150

  • 座長(Chairmanship) 国際学術貢献

    4th ASM Conference on Cell-cell Communication in Bacteria  ( マイアミ ) 2011年11月

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    種別:大会・シンポジウム等 

  • 座長(Chairmanship) 国際学術貢献

    International Union of Microbiological Societies 2011 Congress  ( 札幌 ) 2011年9月

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    種別:大会・シンポジウム等 

  • Bioscience of Microbiota, Food and Health

    2011年9月 - 2025年3月

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    種別:学会・研究会等 

  • 大会総務

    日本生物工学会第62回大会  ( 宮崎 ) 2010年10月

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    種別:大会・シンポジウム等 

    参加者数:2,000

  • アドバイザリー 国際学術貢献

    3rd ASM conference on Enterococci  ( ポートランド ) 2010年8月

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    種別:大会・シンポジウム等 

    参加者数:200

  • 座長(Chairmanship) 国際学術貢献

    3rd ASM Conference on Enterococci  ( オレゴン州・ポートランド ) 2010年8月

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    種別:大会・シンポジウム等 

  • 座長(Chairmanship)

    第16回腸内細菌学会  ( 神戸 ) 2010年6月

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    種別:大会・シンポジウム等 

  • 座長(Chairmanship)

    日本農芸化学会  ( 東京・東京農業大学 ) 2010年3月

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    種別:大会・シンポジウム等 

  • 座長(Chairmanship)

    日本農芸化学会  ( 東京・東京農業大学 ) 2009年3月

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    種別:大会・シンポジウム等 

  • 実行委員

    日本生物工学会九州支部市民フォーラム  ( 福岡 ) 2008年8月

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    種別:大会・シンポジウム等 

    参加者数:70

  • 座長(Chairmanship)

    日本農芸化学会  ( 東京・東京農業大学 ) 2008年3月

     詳細を見る

    種別:大会・シンポジウム等 

  • 座長(Chairmanship)

    日本農芸化学会  ( 東京・東京農業大学 ) 2007年3月

     詳細を見る

    種別:大会・シンポジウム等 

  • 座長(Chairmanship)

    日本農芸化学会  ( 京都・京都大学 ) 2006年3月

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    種別:大会・シンポジウム等 

  • 座長(Chairmanship)

    日本農芸化学会  ( 札幌・北海道大学 ) 2005年3月

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    種別:大会・シンポジウム等 

  • 司会(Moderator)

    乳酸菌と健康  ( 福岡・九州大学 ) 2004年12月

     詳細を見る

    種別:大会・シンポジウム等 

  • 実行委員長

    日本学術会議・日本乳酸菌学会合同セミナー  ( 福岡 ) 2004年12月

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    種別:大会・シンポジウム等 

    参加者数:180

  • 座長(Chairmanship)

    日本乳酸菌学会  ( 仙台・東北大学 ) 2004年7月

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    種別:大会・シンポジウム等 

  • 座長(Chairmanship)

    日本農芸化学会  ( 広島・広島大学 ) 2004年3月

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    種別:大会・シンポジウム等 

  • オーガナイザー

    日本乳酸菌学会秋季セミナー  ( 東京 ) 2003年11月

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    種別:大会・シンポジウム等 

    参加者数:130

▼全件表示

共同研究・競争的資金等の研究課題

  • 食腸育

    2024年

    アジア・オセアニア研究教育機構モジュール

      詳細を見る

    担当区分:研究代表者  資金種別:学内資金・基金等

  • 自己免疫性ぶどう膜炎に対する腸内細菌由来代謝産物を用いた治療法の開発

    研究課題/領域番号:23K18330  2023年6月 - 2025年3月

    科学研究費助成事業  挑戦的研究(萌芽)

    柴田 健輔, 園田 康平, 元岡 大祐, 中山 二郎, 真野 純一

      詳細を見る

    資金種別:科研費

    本研究では、独自に見出した腸内細菌による眼内免疫制御の視点から(Yamana S.,Shibata K.,Mucosal Immunol.,2022)、自己免疫性ぶどう膜炎により失明する患者を救うための新規治療法の開発に挑む。

    CiNii Research

  • 腸ー口腔連関を介した歯周病の免疫応答調節機構の解明と新たな治療法の開発

    研究課題/領域番号:23H00442  2023年4月 - 2026年3月

    科学研究費助成事業  基盤研究(A)

    田中 芳彦, 中山 二郎, 坂上 竜資, 岸川 咲吏, 吉永 泰周

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    資金種別:科研費

    歯周病は歯を失う最大の原因であり、ヒト歯周病医療を視野に入れた基礎的研究の社会的要請が高まっている。歯周病では、歯周病原細菌に応答してインターロイキン17Aを産生するヘルパーT細胞Th17を介した免疫応答による病態悪化が注目されているが、その免疫応答から病態悪化に至る機構は十分に解明されていない。本研究は、歯周病における腸内細菌叢と責任Th17細胞による免疫制御の解析から腸-口腔連関を介した歯周病の免疫応答調節機構を解明し、細菌叢を標的としたヒト歯周病の新しい治療法を開発する。この研究成果からヒト歯周病医療の臨床応用への道が拓かれることが期待される。

    CiNii Research

  • アジアにおける食と健康を繋ぐ腸内フローラの研究拠点の形成 国際共著

    2022年4月 - 2025年3月

    九州大学農学研究院(日本) 

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    担当区分:研究代表者 

    多様で固有なアジア食を、腸内フローラを起点とする健康維持に向けて次世代に継承することを目的に、アジア5か国の食健康医学と腸内細菌学の英知を結集した「アジア食と腸内フローラの研究拠点」をここに構築する。具体的には、腸内フローラ研究の先進国である日本にプロジェクトの拠点を構え、現代食侵襲の問題を抱えるインドネシア、タイ、フィリピン、モンゴルに各国の研究拠点を構える。日本側の拠点は、すでにAsian Microbiome Project(AMP)の拠点として豊富な研究実績を有し、腸内細菌・マイクロバイオームの解析技術が整備されている九州大学に構える。そこに栄養疫学(中村学園大学)、生活習慣病(九大医)、食とエピゲノム(早大)、アジア諸地域の生活環境変化の調査(明治大学)、海外微生物資源(東京農業大学)、の専門家が参加し、アジア諸国における調査をサポートする体制を整える。そして、日本とアジア各国との共同研究により各国のコホート調査を実施する。具体的には、まずマイクロバイオームの解析プロトコルを整備した上で(R1), 旧来の伝統的食習慣の残る地方と現代食の侵襲の激しい都市部の住民[成長期にある小学生児童(R2)と生活習慣病リスクの高い成人(R3)]を対象に、食と腸内フローラと健康の調査を行う。そして、我々が10年前に行った調査結果と比較し、また地域・国間でデータを比較し、伝統食から現代食への移行が、腸内フローラと健康に与えている影響を四次元軸で解析する。同時に、腸内フローラに有益なアジアの食と腸内菌を、未来に継承するためにデータベース化し、加えて、有益菌の菌株コレクションを整備する(R4)。以上の拠点事業には、日本と海外双方の若手研究者や大学院生を積極的に参加させ、この分野横断的な国際コホート調査のイロハを体験させ、当該分野の次世代のリーダーを数多く養成する。本年度は、以上の研究プロジェクト開始のために、まず、共同研究者の最も多いタイ国を訪問し、今後の研究計画について話し合いの機会を持つ。また9月に日本でキックオフミーティングを開催する。4か国からの各2名(できれば若手研究者や学生も含める)を招聘する。日本の参加者と本学の学生にも多く参加してもらい、情報と意見交換を十分に行う。

  • C型非代償性肝硬変やHCV排除後の病態進展に関与する腸肝軸のメカニズムの解明

    研究課題/領域番号:22K08037  2022年4月 - 2025年3月

    科学研究費助成事業  基盤研究(C)

    井上 貴子, 中山 二郎, 田中 靖人

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    資金種別:科研費

    HCV排除(SVR)達成後のC型慢性肝炎、特にC型非代償性肝硬変の病態進展に関与する腸肝軸の変化を、門脈血成分・肝組織での胆汁酸代謝酵素発現量を含めて明らかにし、非アルコール性脂肪性肝疾患と比較しながら、SVR達成後の病態(発癌・線維化・代謝障害)に寄与する要因を同定する。最終的にはSVR達成後の効率的な観察手法の確立・包括的治療の構築につながる腸肝軸モデルの実用化及び開発を目指す。

    CiNii Research

  • アジア食と健康を繋ぐ腸内フローラの研究拠点の形成

    2022年 - 2024年

    科学研究費助成事業  国際学術研究

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    担当区分:研究代表者  資金種別:科研費

  • アジア食と健康を繋ぐ腸内フローラ研究の拠点形成

    2022年 - 2024年

    科学研究費助成事業  研究拠点形成事業

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    担当区分:研究代表者  資金種別:科研費以外の競争的資金

  • アジア食と健康を繋ぐ腸内フローラ研究の拠点形成事業

    2022年 - 2024年

    日本学術振興会  研究拠点形成事業(Bアジアアフリカ学術基盤形成型)

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    担当区分:研究代表者  資金種別:共同研究

  • エピゲノム修飾をマーカーとした腸内フローラにおける糖尿病合併症制御因子の探索

    研究課題/領域番号:22K19136  2022年 - 2023年

    日本学術振興会  科学研究費助成事業  挑戦的研究(萌芽)

    中山 二郎, 井上 貴子, 中尾 洋一

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    担当区分:研究代表者  資金種別:科研費

    腸内フローラは体内から24時間365日何10年間に及び人体に作用し続けるにも関わらず、その研究の多くは、一過的な影響を計るものがほとんどである。そこで、申請者らは、長期に及ぶ遺伝子発現制御に関わるエピゲノム修飾に着眼して、2型糖尿病が招く血管障害に起因する合併症の誘発あるいは抑制に関わる腸内フローラの因子探索を行う。本研究で明らかにされる、腸内フローラ成分によるエピジェネティックな遺伝子スイッチのオン・オフ情報は、長期にわたる高血糖状態に起因する血管障害に、腸内フローラが如何に関連しているかについて初めて明らかにするものである。

    CiNii Research

  • 一般研究助成/各種胆汁酸分子の発芽誘導能を用いた難培養性腸内細菌の分離と日本人腸内細菌叢カクテルの創製

    2021年

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    資金種別:寄附金

  • 研究費助成/合成腸内細菌叢を用いたプレ・プロバイオティクスのインビトロ機能解析システムの構築

    2021年

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    資金種別:寄附金

  • モンゴル遊牧民の食習慣の変化が腸内フローラへ及ぼす影響

    研究課題/領域番号:20H01391  2020年4月 - 2024年3月

    科学研究費助成事業  基盤研究(B)

    森永 由紀, 尾崎 孝宏, 中山 二郎

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    資金種別:科研費

    モンゴル国の草原部は、畜産品に依存する伝統的食習慣が現存する稀に見る地域であるが、市場経済体制移行後は人口の半分以上が西洋化した首都に暮らす。本研究では、食習慣の変化が健康指標の1つである腸内フローラに及ぼす影響を明らかにする。
    課題1: 畜産品の摂取割合と腸内フローラを、都市住民(畜産品摂取量少・中グループ)と草原の遊牧民(多グループ)で比較する。
    課題2: 伝統食で効能の知られるアイラグ(発酵馬乳)が多飲される名産地にて、摂取量による腸内フローラの違いを明らかにする。
    課題3: 近年の製法の変化によるアイラグの微生物の菌叢の変化を、次世代シーケンサーによるメタ16S rRNA解析で解明する。

    CiNii Research

  • 歯周病の病態メカニズムに基づく先進的免疫療法の基盤研究

    研究課題/領域番号:20H00555  2020年4月 - 2023年3月

    科学研究費助成事業  基盤研究(A)

    田中 芳彦, 小笠原 康悦, 中山 二郎, 神沼 修, 永尾 潤一, 岸川 咲吏, 有田 健一, 成田 由香

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    資金種別:科研費

    歯周病において、インターロイキン-17の産生を特徴とするヘルパーT細胞Th17による免疫応答が病態を悪化させることが明らかになってきました。しかし、その病態は充分に解明されておらず、基礎的研究が必要とされています。本研究は、歯周病における腸内細菌と責任Th17細胞による免疫制御機構の解析から歯周病の病態メカニズムを解明します。その理解に立脚して、新しい歯周病疾患モデル動物を創生・検証し、先進的免疫療法の基盤を確立します。このような研究成果を基盤にして、ヒト歯周病の新しい予防法・治療法の開発への展開が期待されます。

    CiNii Research

  • 発酵食品に含まれるスフィンゴ脂質が腸内細菌叢に及ぼすプレバイオティクス効果の解析

    研究課題/領域番号:20K05809  2020年4月 - 2023年3月

    科学研究費助成事業  基盤研究(C)

    北垣 浩志, 中山 二郎

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    資金種別:科研費

    本研究では、麹グリコシルセラミドを加えると腸内細菌叢にどのような影響があるか、麹グリコシルセラミドのどの化学構造がこのような効果をもたらすかをNGSやRTPCRなどの最先端解析技術と独自開発した培養システムを使って明らかにする。これらの研究により、麹グリコシルセラミドのどの化学的構造がどの腸内細菌の増殖あるいは抑制に寄与するのかが明らかになることが期待される。

    CiNii Research

  • アジア人の食と腸内フローラと生活習慣病に関する国際共同調査

    研究課題/領域番号:20KK0130  2020年 - 2023年

    日本学術振興会  科学研究費助成事業  国際共同研究強化(B)

    中山 二郎, 森永 由紀, 町田 光史, 善藤 威史, 中尾 洋一

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    担当区分:研究代表者  資金種別:科研費

    インドネシア、タイ、フィリピン、モンゴル各国に特徴的な食品の直接的あるいは腸内フローラを介する抗生活習慣病効果(特に抗2型糖尿病効果)について、オミックス解析にセルベースのエピゲノム解析を加えて網羅的に解析する。また、各国被験者の糞便や発酵食品から抗糖尿病効果を示す有益菌を分離し、ゲノム解析と抗糖尿病機能解析を行う。そして、各国の伝統的食文化の重要性を改めて認識し、食のグローバル化にアジア人の体をどのように適応させていくべきか、腸内フローラの観点から提言することを目的とする。

    CiNii Research

  • 第2回農芸化学中小企業産学・産官連携研究助成/あまおう由来乳酸菌と沖縄もずくフコイダンの腸内フローラ改善効果

    2020年

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    資金種別:寄附金

  • 平和中島財団・アジア地域重点学術研究助成/モンゴル国における都市化が腸内細菌叢と健康に与える影響の調査

    2020年

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    資金種別:寄附金

  • シェーグレン症候群、IgG4関連涙腺唾液腺炎における腸内細菌叢と病態の関係の解明

    研究課題/領域番号:19K10315  2019年4月 - 2024年3月

    科学研究費助成事業  基盤研究(C)

    大山 順子, 森山 雅文, 中山 二郎, 梶岡 俊一

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    資金種別:科研費

    腸内細菌叢の変化に伴う腸管免疫の変化が全身の免疫調整ネットワークの変調を引き起こすという種々の報告に基づき、腸内細菌叢の変化に伴う免疫系の変調がシェーグレン症候群(SS)、IgG4 関連涙腺唾液腺炎 (IgG4DS)の発症、 病態の進展にどのように関与しているかを検討する。それぞれの疾患について診断時の腸内細菌叢の構成異常の解析に加えて、治療による病態の変化と腸内細菌叢の関係を検討する。またモデルマウスを用いて腸内細菌叢を変化させることでどのように唾液腺炎が変化するかを検討し、腸内細菌叢のコントロールと唾液腺炎の関係も解析する。

    CiNii Research

  • 環状ペプチドクオルモンを用いた腸内細菌の新奇共生戦略

    研究課題/領域番号:19K22288  2019年 - 2020年

    日本学術振興会  科学研究費助成事業  挑戦的研究(萌芽)

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    担当区分:研究代表者  資金種別:科研費

  • 現代食による腸内細菌叢偏倚の実態調査と機構解明

    2019年

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    資金種別:寄附金

  • 現代食による腸内細菌叢偏倚の実態調査と機構解明

    2018年

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    資金種別:寄附金

  • アジアにおける食と腸内細菌叢と生活習慣病に関する調査研究

    2018年

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    資金種別:寄附金

  • Asian Microbiome Project 国際共著

    2017年10月 - 2024年3月

    明治大学、早稲田大学、カセサート大学(タイ)、マヒドン大学(タイ)、フィリピン大学マニラ校(フィリピン)、ガジャマダ大学(インドネシア)、モンゴル生命科学大学(モンゴル)、シンガポール国立大学(シンガポール) 

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    担当区分:研究代表者 

    アジアの多様で優良な食文化は我々アジア人の健康を支えてきた。しかし、アジア人の食習慣は近年の近代化とともに急速に変化し、それに伴い生活習慣病が急増するなど、アジア人の健康は変調をきたしている。その中で、我々はアジア人の腸内フローラを調査し、食と健康を仲介する腸内フローラが、この現代食の影響下にグローバルな変化、すなわちエンテロタイプのシフトを起こしていることを示してきた。ここでは、これまでの国際共同研究をさらに強化し、古来の食文化の直接的あるいは腸内フローラを介する保健効果を、科学的エビデンスをもって実証展開するための研究基盤を構築する。本研究基盤構築のアプトプットとして、アジア各国特有の食成分の作用を顕在化させるとともに、国を超えた共通のFood-Microbiome-Human axisを明らかにすることを目的とする。具体的には、インドネシア、タイ、フィリピン、モンゴル各国に特徴的な食品の、直接的あるいは腸内フローラを介する抗生活習慣病効果(特に抗2型糖尿病)について、オミックス解析にセルベースのエピゲノム解析を加えて網羅的に解析する。そして、各国の伝統的食文化の重要性を改めて認識し、食のグローバル化にアジア人の体をどのように適応させていくべきか、腸内フローラの観点から提言する。

  • 世界の健康に貢献する日本食パターンの評価方法の確立とその応用に関する研究

    2017年10月 - 2020年3月

    東北大学、京都大学、岡山県立大学、理化学研究所、九州大学、慶應義塾大学、国立長寿医療研究センター 

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    担当区分:研究分担者 

    日本食は食事内容のスコア化による客観的な評価法の開発などの取組があまりなされておらず、科学的エビデンスの充実をはかり、日本食の健康有益性に関する情報を国際的に発信していく必要がある。本研究では、発表済みの日本食スコアをベースに、日本食の有益性や改善点を明らかにしたうえで、食材だけではなく、多様性、形式、調理法、調味料などに関しても盛り込むよう改良する。その日本食スコアに関して、健康増進効果の関連性について、動物試験やヒトデータで検証し、日本食スコアを完成させる。さらに、改良版日本食スコアを活用したヒト介入試験を行い、その有用性を証明する。また、改良日本食スコアを活用し、社会実装を促進する。

  • 食と腸内細菌と健康に関するアジア横断研究

    研究課題/領域番号:17H04620  2017年 - 2019年

    日本学術振興会  科学研究費助成事業  基盤研究(B)

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    担当区分:研究代表者  資金種別:科研費

  • 腸内細菌のクオラムセンシングの実態解明と疾病予防および健康増進に向けた制御

    研究課題/領域番号:15H04480  2015年 - 2017年

    日本学術振興会  科学研究費助成事業  基盤研究(B)

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    担当区分:研究代表者  資金種別:科研費

  • アジア人種の食と腸内フローラと健康に関する調査研究

    研究課題/領域番号:25304006  2013年 - 2015年

    日本学術振興会  科学研究費助成事業  基盤研究(B)

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    担当区分:研究代表者  資金種別:科研費

  • グラム陽性細菌のクオラムセンシングを標的とした抗感染症剤の開発

    研究課題/領域番号:24380050  2012年 - 2014年

    日本学術振興会  科学研究費助成事業  基盤研究(B)

      詳細を見る

    担当区分:研究代表者  資金種別:科研費

  • アジア人の食と腸内細菌と健康に関する調査研究

    2012年 - 2013年

    九州大学教育研究プログラム・研究拠点形成プロジェクト(P&P)

      詳細を見る

    担当区分:研究代表者  資金種別:学内資金・基金等

  • ヒト乳幼児における腸内細菌叢形成と宿主免疫系発達の相互作用機序に関する研究

    研究課題/領域番号:23651207  2011年 - 2013年

    科学研究費助成事業  挑戦的萌芽研究

      詳細を見る

    担当区分:研究代表者  資金種別:科研費

  • グラム陽性細菌のクオラムセンシングを標的とした眼内炎予防治療薬の開発

    研究課題/領域番号:AS232Z02064G  2011年 - 2012年

    科学研究費助成事業  (独)科学技術振興機構 研究成果展開事業研究成果最適展開支援プログラムフィージビリティスタディステージ

      詳細を見る

    担当区分:研究代表者  資金種別:科研費以外の競争的資金

  • アジア人種の腸内フローラ基盤データベースの構築

    2010年

      詳細を見る

    資金種別:寄附金

  • 環状ペプチドクォルモンの生合成酵素および受容体を標的とした抗感染症剤の開発

    研究課題/領域番号:21380061  2009年 - 2011年

    日本学術振興会  科学研究費助成事業  基盤研究(B)

      詳細を見る

    担当区分:研究代表者  資金種別:科研費

  • 学術研究助成奨励金/乳幼児期の腸内フローラ形成と食物アレルギー発症の関連性の解析

    2009年

      詳細を見る

    資金種別:寄附金

  • アジア人種の腸内フローラ基盤データベースの構築

    2009年

      詳細を見る

    資金種別:寄附金

  • 地域イノベーション創出総合支援事業「シーズ発掘試験」/乳幼児期の種々疾病予知・予防のための腸内フローラ解析システムの構築」

    2008年4月 - 2009年3月

    個人研究

      詳細を見る

    担当区分:研究代表者  資金種別:その他産学連携による資金

  • 学術研究助成奨励金/食物アレルギー予知・予防のための乳幼児腸内フローラ解析システムの構築

    2008年

      詳細を見る

    資金種別:寄附金

  • グラム陽性細菌のクォーラムセンシングを標的とした新規抗菌剤の開発と応用

    研究課題/領域番号:19380053  2007年 - 2008年

    日本学術振興会  科学研究費助成事業  基盤研究(B)

      詳細を見る

    担当区分:研究代表者  資金種別:科研費

  • グラム陽性細菌のクォーラムセンシングを標的とした新規抗菌剤の開発と応用

    研究課題/領域番号:19380053  2007年 - 2008年

    日本学術振興会  科学研究費助成事業  基盤研究(B)

      詳細を見る

    担当区分:研究代表者  資金種別:科研費

  • 腸内フローラにおける細菌間相互作用の総合的理解に向けての萌芽的研究

    研究課題/領域番号:19658034  2007年 - 2008年

    科学研究費助成事業  萌芽研究

      詳細を見る

    担当区分:研究代表者  資金種別:科研費

  • 学術研究助成奨励金/グラム陽性細菌の環状ペプチドクォルモンの生合成を標的とする抗菌剤の創製

    2007年

      詳細を見る

    資金種別:寄附金

  • 乳酸菌バクテリオシンを利用した乳房炎予防・治療抗菌剤の開発

    2006年4月 - 2009年3月

    九州大学大学院農学研究院 

      詳細を見る

    担当区分:研究分担者 

  • 食品機能性対応指標基づく階層的バイオプロセス制御の開発

    2006年4月 - 2008年3月

    九州大学大学院農学研究院 

      詳細を見る

    担当区分:研究分担者 

  • 乳酸菌バクテリオシンを利用した乳房炎予防・治療抗菌剤の開発

    2006年 - 2008年

    農林水産省高度化事業

      詳細を見る

    担当区分:研究分担者  資金種別:受託研究

  • 食品機能性対応指標基づく階層的バイオプロセス制御の開発

    2006年 - 2007年

    地域新生コンソーシアム

      詳細を見る

    担当区分:研究分担者  資金種別:受託研究

  • 加藤記念研究助成/グラム陽性菌のクォーラムセンシングを標的とした新奇抗感染症剤の創製

    2006年

      詳細を見る

    資金種別:寄附金

  • グラム陽性細菌のクォーラムセンシングを標的とした新奇抗菌剤の開発

    研究課題/領域番号:17580068  2005年 - 2006年

    日本学術振興会  科学研究費助成事業  基盤研究(C)

      詳細を見る

    担当区分:研究代表者  資金種別:科研費

  • 若手学術研究者支援/複合土壌細菌群リサイクルシステムにおける菌叢モニタリング

    2005年

      詳細を見る

    資金種別:寄附金

  • 地域集団でのコホート研究による便中細菌診断妥当性の研究

    2004年4月 - 2006年3月

    厚生労働省 

      詳細を見る

    担当区分:研究分担者 

    熊本県小国町にて出生する新生児を対象にビフィズス菌の経口投与の介入試験を行い、便中細菌叢とアレルギー発症を調査し、両者の間の関連性を実験疫学的に解析する。

  • 地域集団でのコホート研究による便中細菌診断妥当性の研究

    研究課題/領域番号:H13-免疫-009  2004年 - 2006年

    科学研究費助成事業  厚生労働科学研究費補助金 (厚生労働省)

      詳細を見る

    担当区分:研究分担者  資金種別:科研費以外の競争的資金

  • 学術研究助成金/乳幼児における腸内細菌叢形成とアレルギー罹患についての分子疫学的研究

    2004年

      詳細を見る

    資金種別:寄附金

  • クオーラムセンシングを標的とした抗細菌剤の開発

    研究課題/領域番号:15580065  2003年 - 2004年

    日本学術振興会  科学研究費助成事業  基盤研究(C)

      詳細を見る

    資金種別:科研費

  • 研究助成金/総合ゲノム分子生物学を基盤としたプロバイオティクス乳酸菌の腸管内環境適応定着機構の解析

    2003年

      詳細を見る

    資金種別:寄附金

  • リウマチアレルギー疾患の早期診断に関する研究

    研究課題/領域番号:H16-免疫-002  2002年 - 2003年

    科学研究費助成事業  厚生労働科学研究費補助金 (厚生労働省)

      詳細を見る

    担当区分:研究分担者  資金種別:科研費以外の競争的資金

▼全件表示

教育活動概要

  • 大学院教育
    システム生物工学演習第一、システム生物工学演習第二、システム生物工学ティーチング演習、システム生物工学演示技法、国際交流演習I、国際交流演習II、国際交流演習III、国際交流演習IV、システム生物工学特別研究第一、システム生物工学特別研究第二、システム生物工学プロジェクト演習、微生物生産工学特論、微生物工学特論、国際演示技法、国際交流演習I、国際交流演習II、国際交流演習III、国際交流演習IV、ティーチング演習、プロジェクト演習、プロジェクト演習、システム生物工学特別実験、システム生物工学特別講究、システム生物工学特別演習

    学部教育
    基礎微生物学、微生物工学、微生物基礎実験、微生物工学実験、単位操作実験、卒業研究、科学英語、バイオテクノロジー論、食科学の新展開

    基幹教育
    課題協学、農学入門

担当授業科目

  • 単位操作実験

    2024年10月 - 2025年3月   後期

  • 農学入門Ⅱ

    2024年10月 - 2025年3月   後期

  • 微生物工学

    2024年10月 - 2025年3月   後期

  • 微生物工学特論

    2024年10月 - 2024年12月   秋学期

  • Bio-Engineering Ⅱ

    2024年6月 - 2024年8月   夏学期

  • 卒業研究(食糧化学工学分野)

    2024年4月 - 2025年3月   通年

  • Master's Thesis Research Ⅰ

    2024年4月 - 2025年3月   通年

  • Seminar in a Specified Field Ⅰ

    2024年4月 - 2025年3月   通年

  • 実地見学(食糧化学工学分野)

    2024年4月 - 2024年9月   前期

  • 農学入門Ⅱ

    2024年4月 - 2024年9月   前期

  • バイオテクノロジー論

    2024年4月 - 2024年9月   前期

  • Bio-Engineering Ⅰ

    2024年4月 - 2024年6月   春学期

  • 単位操作実験

    2023年10月 - 2024年3月   後期

  • 微生物工学

    2023年10月 - 2024年3月   後期

  • 微生物工学特論

    2023年10月 - 2023年12月   秋学期

  • 基幹教育セミナー

    2023年6月 - 2023年8月   夏学期

  • システム生物工学特論

    2023年4月 - 2023年6月   春学期

  • Bio-Engineering

    2023年4月 - 2023年6月   春学期

  • 微生物工学

    2022年10月 - 2023年3月   後期

  • 課題協学科目

    2022年10月 - 2023年3月   後期

  • 課題協学科目

    2022年10月 - 2023年3月   後期

  • 微生物工学

    2022年10月 - 2023年3月   後期

  • 単位操作実験

    2022年10月 - 2023年3月   後期

  • 単位操作実験

    2022年10月 - 2023年3月   後期

  • 微生物工学特論

    2022年10月 - 2022年12月   秋学期

  • Master's Thesis

    2022年4月 - 2023年3月   通年

  • Master's Thesis Research Ⅱ

    2022年4月 - 2023年3月   通年

  • Seminar in a Specified Field Ⅱ

    2022年4月 - 2023年3月   通年

  • バイオテクノロジー論

    2022年4月 - 2022年9月   前期

  • システム生物工学特論

    2022年4月 - 2022年6月   春学期

  • Bio-Engineering

    2022年4月 - 2022年6月   春学期

  • システム生物工学プロジェクト演習

    2021年12月 - 2022年2月   冬学期

  • 単位操作実験

    2021年10月 - 2022年3月   後期

  • システム生物工学演習第一

    2021年10月 - 2022年3月   後期

  • システム生物工学演習第二

    2021年10月 - 2022年3月   後期

  • 微生物学基礎実験(食糧化学工学分野)

    2021年10月 - 2022年3月   後期

  • 微生物工学

    2021年10月 - 2022年3月   後期

  • 微生物工学実験

    2021年10月 - 2022年3月   後期

  • 微生物工学特論

    2021年10月 - 2021年12月   秋学期

  • 微生物工学特論

    2021年10月 - 2021年12月   秋学期

  • 修士論文

    2021年4月 - 2022年3月   通年

  • システム生物工学特別研究第一

    2021年4月 - 2022年3月   通年

  • システム生物工学特別研究第二

    2021年4月 - 2022年3月   通年

  • Bio-Engineering

    2021年4月 - 2021年6月   春学期

  • 微生物生産工学特論

    2021年4月 - 2021年6月   春学期

  • Master's Thesis Research Ⅰ

    2021年4月 - 2021年6月   春学期

  • Seminar in a Specified Field Ⅰ

    2021年4月 - 2021年6月   春学期

  • 単位操作実験

    2020年10月 - 2021年3月   後期

  • 基礎微生物学

    2020年10月 - 2021年3月   後期

  • 微生物学基礎実験(食糧化学工学分野)

    2020年10月 - 2021年3月   後期

  • 微生物工学

    2020年10月 - 2021年3月   後期

  • 微生物工学実験

    2020年10月 - 2021年3月   後期

  • 微生物工学特論

    2020年10月 - 2020年12月   秋学期

  • バイオテクノロジー論

    2020年4月 - 2020年9月   前期

  • 微生物生産工学特論

    2020年4月 - 2020年6月   春学期

  • Bio-Engineering

    2020年4月 - 2020年6月   春学期

  • 単位操作実験

    2019年10月 - 2020年3月   後期

  • システム生物工学プロジェクト演習

    2019年10月 - 2020年3月   後期

  • 基礎微生物学

    2019年10月 - 2020年3月   後期

  • 微生物学基礎実験(食糧化学工学分野)

    2019年10月 - 2020年3月   後期

  • 微生物工学

    2019年10月 - 2020年3月   後期

  • 微生物工学実験

    2019年10月 - 2020年3月   後期

  • 微生物工学特論

    2019年10月 - 2019年12月   秋学期

  • システム生物工学演習第一

    2019年4月 - 2020年3月   通年

  • 農学入門II

    2019年4月 - 2020年3月   通年

  • システム生物工学特別研究第二

    2019年4月 - 2020年3月   通年

  • システム生物工学特別研究第一

    2019年4月 - 2020年3月   通年

  • システム生物工学ティーチング演習

    2019年4月 - 2020年3月   通年

  • システム生物工学演習第二

    2019年4月 - 2019年9月   前期

  • 食科学の新展開

    2019年4月 - 2019年9月   前期

  • 微生物生産工学特論

    2019年4月 - 2019年6月   春学期

  • Bio-Engineering

    2019年4月 - 2019年6月   春学期

  • 微生物工学

    2018年10月 - 2019年3月   後期

  • 基礎微生物学

    2018年10月 - 2019年3月   後期

  • 微生物学基礎実験(食糧化学工学分野)

    2018年10月 - 2019年3月   後期

  • 微生物工学実験

    2018年10月 - 2019年3月   後期

  • 単位操作実験

    2018年10月 - 2019年3月   後期

  • 微生物生産工学特論

    2018年4月 - 2018年6月   春学期

  • 単位操作実験

    2017年10月 - 2018年3月   後期

  • 基礎微生物学

    2017年10月 - 2018年3月   後期

  • 微生物工学実験

    2017年10月 - 2018年3月   後期

  • 単位操作実験

    2017年10月 - 2018年3月   後期

  • 微生物学基礎実験(食糧化学工学分野)

    2017年10月 - 2018年3月   後期

  • 微生物工学実験

    2017年10月 - 2018年3月   後期

  • 基幹教育セミナー

    2017年6月 - 2017年8月   夏学期

  • 科学英語II

    2017年4月 - 2017年9月   前期

  • バイオマテリアル特論

    2017年4月 - 2017年9月   前期

  • Bio-engineering

    2017年4月 - 2017年9月   前期

  • 分子微生物学・バイオマス資源化学プロジェクト演習

    2017年4月 - 2017年9月   前期

  • バイオマテリアル特論

    2017年4月 - 2017年9月   前期

  • Master's Thesis

    2017年4月 - 2017年6月   春学期

  • Master’s Thesis ResearchⅠ

    2017年4月 - 2017年6月   春学期

  • Master's Thesis Research II

    2017年4月 - 2017年6月   春学期

  • 単位操作実験

    2016年10月 - 2017年3月   後期

  • 微生物工学実験

    2016年10月 - 2017年3月   後期

  • 科学英語II

    2016年4月 - 2016年9月   前期

  • Bio-engineering

    2016年4月 - 2016年9月   前期

  • 分子微生物学・バイオマス資源化学プロジェクト演習

    2016年4月 - 2016年9月   前期

  • バイオマテリアル特論

    2016年4月 - 2016年9月   前期

  • 課題協学B

    2016年4月 - 2016年9月   前期

  • 単位操作実験

    2015年10月 - 2016年3月   後期

  • 微生物工学実験

    2015年10月 - 2016年3月   後期

  • 科学英語II

    2015年4月 - 2015年9月   前期

  • Bio-engineering

    2015年4月 - 2015年9月   前期

  • 分子微生物学・バイオマス資源化学プロジェクト演習

    2015年4月 - 2015年9月   前期

  • バイオマテリアル特論

    2015年4月 - 2015年9月   前期

  • 課題協学B

    2015年4月 - 2015年9月   前期

  • 微生物工学実験

    2014年10月 - 2015年3月   後期

  • 基礎微生物学

    2014年10月 - 2015年3月   後期

  • 単位操作実験

    2014年10月 - 2015年3月   後期

  • Bio-engineering

    2014年4月 - 2014年9月   前期

  • 科学英語II

    2014年4月 - 2014年9月   前期

  • 課題協学B

    2014年4月 - 2014年9月   前期

  • バイオマテリアル特論

    2014年4月 - 2014年9月   前期

  • 分子微生物学・バイオマス資源化学プロジェクト演習

    2014年4月 - 2014年9月   前期

  • 細胞生物学

    2013年10月 - 2014年3月   後期

  • 微生物工学実験

    2013年10月 - 2014年3月   後期

  • 単位操作実験

    2013年10月 - 2014年3月   後期

  • 基礎化学B

    2013年4月 - 2013年9月   前期

  • Bio-engineering

    2013年4月 - 2013年9月   前期

  • 分子微生物学・バイオマス資源化学プロジェクト演習

    2013年4月 - 2013年9月   前期

  • バイオマテリアル特論

    2013年4月 - 2013年9月   前期

  • 自然科学総合実験

    2013年4月 - 2013年9月   前期

  • 科学英語II

    2013年4月 - 2013年9月   前期

  • 微生物工学実験

    2012年10月 - 2013年3月   後期

  • 基礎微生物学

    2012年10月 - 2013年3月   後期

  • 細胞生物学

    2012年10月 - 2013年3月   後期

  • 単位操作実験

    2012年10月 - 2013年3月   後期

  • 科学英語II

    2012年4月 - 2012年9月   前期

  • 分子微生物学・バイオマス資源化学プロジェクト演習

    2012年4月 - 2012年9月   前期

  • バイオマテリアル特論

    2012年4月 - 2012年9月   前期

  • 基礎化学B

    2012年4月 - 2012年9月   前期

  • バイオマテリアル特論

    2011年10月 - 2012年3月   後期

  • 微生物工学実験

    2011年10月 - 2012年3月   後期

  • 遺伝子工学実験

    2011年10月 - 2012年3月   後期

  • 単位操作実験

    2011年10月 - 2012年3月   後期

  • 細胞生物学

    2011年10月 - 2012年3月   後期

  • 基礎化学B

    2011年4月 - 2011年9月   前期

  • 科学英語II

    2011年4月 - 2011年9月   前期

  • 微生物工学実験

    2010年10月 - 2011年3月   後期

  • 基礎微生物学

    2010年10月 - 2011年3月   後期

  • バイオマテリアル特論

    2010年10月 - 2011年3月   後期

  • 細胞生物学

    2010年10月 - 2011年3月   後期

  • 単位操作実験

    2010年10月 - 2011年3月   後期

  • 遺伝子工学実験

    2010年10月 - 2011年3月   後期

  • 科学英語II

    2010年4月 - 2010年9月   前期

  • 基礎化学B

    2010年4月 - 2010年9月   前期

  • 食品微生物解析学特論

    2009年10月 - 2010年3月   後期

  • 科学英語II

    2009年10月 - 2010年3月   後期

  • 細胞生物学

    2009年10月 - 2010年3月   後期

  • 単位操作実験

    2009年10月 - 2010年3月   後期

  • 遺伝子工学実験

    2009年10月 - 2010年3月   後期

  • 微生物工学実験

    2009年10月 - 2010年3月   後期

  • 食品微生物解析学特論

    2009年10月 - 2010年3月   後期

  • 科学英語II

    2009年10月 - 2010年3月   後期

  • 細胞生物学

    2009年10月 - 2010年3月   後期

  • 単位操作実験

    2009年10月 - 2010年3月   後期

  • 遺伝子工学実験

    2009年10月 - 2010年3月   後期

  • 微生物工学実験

    2009年10月 - 2010年3月   後期

  • 科学英語II

    2009年4月 - 2009年9月   前期

  • 基礎化学B

    2009年4月 - 2009年9月   前期

  • 生物機能科学通論

    2009年4月 - 2009年9月   前期

  • 科学英語II

    2009年4月 - 2009年9月   前期

  • 基礎化学B

    2009年4月 - 2009年9月   前期

  • 生物機能科学通論

    2009年4月 - 2009年9月   前期

  • 細胞生物学

    2008年10月 - 2009年3月   後期

  • 食品微生物解析学特論

    2008年10月 - 2009年3月   後期

  • 微生物工学実験

    2008年10月 - 2009年3月   後期

  • 遺伝子工学実験

    2008年10月 - 2009年3月   後期

  • 単位操作実験

    2008年10月 - 2009年3月   後期

  • 基礎微生物学

    2008年10月 - 2009年3月   後期

  • 細胞生物学

    2008年10月 - 2009年3月   後期

  • 食品微生物解析学特論

    2008年10月 - 2009年3月   後期

  • 微生物工学実験

    2008年10月 - 2009年3月   後期

  • 遺伝子工学実験

    2008年10月 - 2009年3月   後期

  • 単位操作実験

    2008年10月 - 2009年3月   後期

  • 基礎微生物学

    2008年10月 - 2009年3月   後期

  • 基礎化学B

    2008年4月 - 2008年9月   前期

  • 生物機能科学通論

    2008年4月 - 2008年9月   前期

  • 基礎化学B

    2008年4月 - 2008年9月   前期

  • 生物機能科学通論

    2008年4月 - 2008年9月   前期

  • 微生物工学実験・遺伝子工学実験・単位操作実験

    2007年10月 - 2008年3月   後期

  • 食品微生物解析学特論

    2007年10月 - 2008年3月   後期

  • 微生物工学実験・遺伝子工学実験・単位操作実験

    2007年10月 - 2008年3月   後期

  • 食品微生物解析学特論

    2007年10月 - 2008年3月   後期

  • 生物機能科学通論

    2007年4月 - 2007年9月   前期

  • 生物機能科学通論

    2007年4月 - 2007年9月   前期

  • 微生物工学実験・遺伝子工学実験・単位操作実験

    2006年10月 - 2007年3月   後期

  • 基礎微生物学

    2006年10月 - 2007年3月   後期

  • 微生物工学実験・遺伝子工学実験・単位操作実験

    2006年10月 - 2007年3月   後期

  • 基礎微生物学

    2006年10月 - 2007年3月   後期

  • 生物機能科学通論

    2006年4月 - 2006年9月   前期

  • 生物機能科学通論

    2006年4月 - 2006年9月   前期

  • 微生物工学実験・遺伝子工学実験・単位操作実験

    2005年10月 - 2006年3月   後期

  • 微生物工学実験・遺伝子工学実験・単位操作実験

    2005年10月 - 2006年3月   後期

  • 微生物工学実験・遺伝子工学実験・単位操作実験

    2004年10月 - 2005年3月   後期

  • 基礎微生物学

    2004年10月 - 2005年3月   後期

  • 微生物工学実験・遺伝子工学実験・単位操作実験

    2004年10月 - 2005年3月   後期

  • 基礎微生物学

    2004年10月 - 2005年3月   後期

  • 科学英語I

    2003年10月 - 2004年3月   後期

  • 微生物工学実験・遺伝子工学実験・単位操作実験

    2003年10月 - 2004年3月   後期

  • 科学英語I

    2003年10月 - 2004年3月   後期

  • 微生物工学実験・遺伝子工学実験・単位操作実験

    2003年10月 - 2004年3月   後期

  • 微生物工学実験・遺伝子工学実験・単位操作実験

    2002年10月 - 2003年3月   後期

  • 基礎微生物学

    2002年10月 - 2003年3月   後期

  • 科学英語I

    2002年10月 - 2003年3月   後期

  • 微生物工学実験・遺伝子工学実験・単位操作実験

    2002年10月 - 2003年3月   後期

  • 基礎微生物学

    2002年10月 - 2003年3月   後期

  • 科学英語I

    2002年10月 - 2003年3月   後期

▼全件表示

FD参加状況

  • 2024年3月   役割:参加   名称:有体物管理センターの業務および 成果有体物収入の配分率の変更について

    主催組織:部局

  • 2023年11月   役割:参加   名称:遺伝子組換え実験の安全管理について

    主催組織:部局

  • 2022年6月   役割:参加   名称:課題協学

    主催組織:全学

  • 2022年3月   役割:参加   名称:国費特別プログラム「未来の農を描くビジョンデザイン実践プログラム」実施に向けて

    主催組織:部局

  • 2022年3月   役割:参加   名称:リポジトリ登録を対象とした大学改革推進経費の新指標案について

    主催組織:部局

  • 2020年9月   役割:参加   名称:科研費を獲りにいこう! 勝ち抜く気合と技術

    主催組織:部局

  • 2020年9月   役割:参加   名称:科研費を獲りにいこう! 勝ち抜く気合と技術

    主催組織:部局

  • 2020年1月   役割:参加   名称:ジャーナルをめぐる現状と今後に向けてー論文の出版と入手ー

    主催組織:部局

  • 2019年11月   役割:参加   名称:留学生との共生に向けて

    主催組織:部局

  • 2019年9月   役割:参加   名称:コンプライアンス研修

    主催組織:部局

  • 2019年5月   役割:参加   名称:優良な博士人材の獲得と育成に向けて~農学研究院教授に学ぶ~

    主催組織:部局

  • 2019年4月   役割:参加   名称:優良な博士人材の獲得と育成に向けて ~農学研究院教授に学ぶ ~

    主催組織:部局

  • 2018年12月   役割:参加   名称:大学を強くする~大学経営改革

    主催組織:部局

  • 2017年11月   役割:参加   名称:産学官共同研究における費用負担と営業秘密の管理について

    主催組織:部局

  • 2017年3月   役割:参加   名称:m2b(ミツバ)システムの授業への活用

    主催組織:部局

  • 2017年2月   役割:参加   名称:科研費「特設分野研究」について

    主催組織:部局

  • 2016年11月   役割:参加   名称:国際法務室による安全保障輸出管理等リスクマネジメント業務について

    主催組織:部局

  • 2016年2月   役割:参加   名称:企業との共同研究における研究成果のデザイン

    主催組織:部局

  • 2012年12月   役割:参加   名称:近年の学生像の変化と大学における対応について

    主催組織:部局

  • 2010年7月   役割:参加   名称:農学研究院サバティカル(特別研究期間)の運用について

    主催組織:部局

  • 2010年6月   役割:参加   名称:農学研究院における情報セキュリティについて

    主催組織:部局

  • 2010年4月   役割:参加   名称:学府再編に伴うカリキュラムの改正について

    主催組織:部局

  • 2010年1月   役割:参加   名称:九州大学全学シラバスの利用法について

    主催組織:部局

  • 2009年8月   役割:参加   名称:農学研究院部門組織再編の基本

    主催組織:部局

  • 2009年4月   役割:参加   名称:GPA制度について

    主催組織:部局

  • 2009年3月   役割:参加   名称:「英語による授業の質の向上を目指して」

    主催組織:部局

  • 2008年12月   役割:参加   名称:「生物資源環境科学府の組織再編」について

    主催組織:部局

  • 2008年6月   役割:参加   名称:「セクシュアル・ハラスメント、アカデミック・ハラスメント及び男女共同参画」について

    主催組織:部局

  • 2008年5月   役割:参加   名称:「九州大学の教育研究と学生生活に関する大学院学生アンケート」の分析について

    主催組織:部局

  • 2007年12月   役割:参加   名称:学生指導について(ファシリテーション)

    主催組織:部局

  • 2005年5月   役割:参加   名称:学生指導について(アカデミックハラスメント)

    主催組織:部局

  • 2005年3月   役割:参加   名称:農林水産研究基本計画について

    主催組織:部局

  • 2004年7月   役割:参加   名称:学生のメンタルヘルスに関する最近の話題

    主催組織:部局

  • 2003年12月   役割:参加   名称:九州大学における言語文化科目の教育内容の改善に向けて

    主催組織:全学

  • 2003年4月   役割:参加   名称:不明

    主催組織:部局

  • 2002年12月   役割:参加   名称:九州大学における語学教育の展望

    主催組織:部局

▼全件表示

他大学・他機関等の客員・兼任・非常勤講師等

  • 2022年  山口大学医学部  区分:集中講義  国内外の区分:国内 

    学期、曜日時限または期間:後期

  • 2021年  明治大学農学部  区分:集中講義  国内外の区分:国内 

    学期、曜日時限または期間:前期

  • 2021年  大阪大学生物工学国際交流センター  区分:客員教員  国内外の区分:国内 

    学期、曜日時限または期間:後期

  • 2020年  東京農業大学  区分:非常勤講師  国内外の区分:国内 

    学期、曜日時限または期間:前期、後期

  • 2019年  (独)製品評価技術基盤機構  区分:客員教員  国内外の区分:国内 

  • 2018年  (独)製品評価技術基盤機構  区分:客員教員  国内外の区分:国内 

  • 2017年  (独)製品評価技術基盤機構  区分:客員教員  国内外の区分:国内 

    学期、曜日時限または期間:2018年2月より

  • 2015年  東京大学大学院農学生命科学研究科  区分:非常勤講師  国内外の区分:国内 

    学期、曜日時限または期間:後期

  • 2011年  東京大学大学院農学生命科学研究科  区分:非常勤講師  国内外の区分:国内 

    学期、曜日時限または期間:後期

  • 2007年  大阪大学生物工学国際交流センター・ユネスコバイオテクノロジー国際大学院研修講座  区分:非常勤講師  国内外の区分:国内 

  • 2003年  東和大学  区分:非常勤講師  国内外の区分:国内 

    学期、曜日時限または期間:前期

  • 2002年  東和大学  区分:非常勤講師  国内外の区分:国内 

    学期、曜日時限または期間:前期

▼全件表示

その他教育活動及び特記事項

  • 2024年  クラス担任  学部

  • 2024年  クラス担任  学部

  • 2022年  クラス担任  学部

  • 2016年  クラス担任  学部

  • 2010年  クラス担任  学部

  • 2009年  クラス担任  学部

  • 2003年  クラス担任  学部

  • 2002年  クラス担任  学部

▼全件表示

社会貢献・国際連携活動概要

  • 1996年度・共同研究(キリンビール株式会社)
    2022年度-2024年度・日本学術振興会研究拠点形成事業(B.アジアアフリカ学術基盤形成型)

社会貢献活動

  • 「いとしま免疫村」拠点づくりクリエイティブセミナー

    九州大学・糸島市  糸島健康福祉センター ふれあい  2024年3月

     詳細を見る

    対象:社会人・一般, 学術団体, 企業, 市民団体, 行政機関

    種別:講演会

  • 模擬事業「菌を食べよう・育てよう」

    佐賀県立唐津東中学校  2023年12月

     詳細を見る

    対象:幼稚園以下, 小学生, 中学生, 高校生

    種別:セミナー・ワークショップ

  • 第64回(令和5年度)果汁技術研究発表会特別講演

    日本果汁協会  東京・ニッショーホール  2023年9月

     詳細を見る

    対象:社会人・一般, 学術団体, 企業, 市民団体, 行政機関

    種別:講演会

    「アジア人の食と腸内フローラと健康」について講演する

  • NGS(次世代シーケンサー)を用いた菌叢解析のノウハウ―腸内細菌叢を例に―

    情報機構  リモート  2021年8月

     詳細を見る

    対象:社会人・一般, 学術団体, 企業, 市民団体, 行政機関

    種別:セミナー・ワークショップ

    昨今注目されている腸内細菌叢(腸内フローラ)研究の基盤を支える、
    NGSを用いた菌叢解析について 基本から実際のデータ処理について丸わかり!
    講師の研究データ、サンプルを参照しながら、解析の手順が分かる!

  • アジア人の食習慣と腸内フローラと健康

    西日本機能性食品開発研究会実行委員会  マリンメッセ福岡  2021年5月

     詳細を見る

    対象:社会人・一般, 学術団体, 企業, 市民団体, 行政機関

    種別:セミナー・ワークショップ

  • NGS(次世代シーケンサー)を用いた菌叢解析のノウハウ―腸内細菌叢を例に―

    情報機構  リモート  2021年2月

     詳細を見る

    対象:社会人・一般, 学術団体, 企業, 市民団体, 行政機関

    種別:セミナー・ワークショップ

    昨今注目されている腸内細菌叢(腸内フローラ)研究の基盤を支える、
    NGSを用いた菌叢解析について 基本から実際のデータ処理について丸わかり!
    講師の研究データ、サンプルを参照しながら、解析の手順が分かる!

  • 南区ため蔵食ゼミ

    福岡市南区保健福祉センター  福岡市南区アミカス  2019年3月

     詳細を見る

    対象:社会人・一般, 学術団体, 企業, 市民団体, 行政機関

    種別:講演会

  • 南区ため蔵食ゼミ

    福岡市南区保健福祉センター  福岡市南区アミカス  2019年3月

     詳細を見る

    種別:出前授業

    researchmap

  • 食品開発展2018

    UBMジャパン  東京ビックサイト  2018年10月

     詳細を見る

    対象:社会人・一般, 学術団体, 企業, 市民団体, 行政機関

    種別:講演会

    アジアには固有の食文化が今もなお多く維持されており、中には種々健康効果をもたらすとされるものも多い。Asian Microbiome Projectでは、それらの食と昨今第三の臓器として注目される腸内細菌叢との関連性について調査している。西欧食文化が次々と浸透する中、アジア人の現代食はどうあるべきか考えてみたい。

  • 食品開発展2018

    UBMジャパン  東京ビックサイト  2018年10月

     詳細を見る

    種別:講演会

    アジアには固有の食文化が今もなお多く維持されており、中には種々健康効果をもたらすとされるものも多い。Asian Microbiome Projectでは、それらの食と昨今第三の臓器として注目される腸内細菌叢との関連性について調査している。西欧食文化が次々と浸透する中、アジア人の現代食はどうあるべきか考えてみたい。

    researchmap

  • 九州大学学術研究都市情報交流セミナー

    九州大学学術都市推進機構  ホテルニューオータニ博多  2018年7月

     詳細を見る

    対象:社会人・一般, 学術団体, 企業, 市民団体, 行政機関

    種別:セミナー・ワークショップ

    多民族が固有の文化を保持しながらひしめきあうアジアはユニークで優良な食の宝庫でもある。一方、我々の腸内に宿り第3の臓器とも言われる腸内細菌叢は、食と健康のインターフェースとして近年注目され我々の健康に重要な働きをしていることが分かってきた。そこで九州大学農学研究院微生物工学研究室では、アジア諸国と共同で“Asian Microbiome Project”を展開し、アジア人の食と腸内細菌叢と健康を調査している。その結果、西洋化の中、変わりゆくアジア人の腸内細菌叢の姿が見えてきた。その中で、日本人の腸内細菌叢はどのようになっているのか、そしてそれが我々の健康にどのように関わっているのか解説する。

  • 九州大学学術研究都市情報交流セミナー

    九州大学学術都市推進機構  ホテルニューオータニ博多  2018年7月

     詳細を見る

    種別:セミナー・ワークショップ

    多民族が固有の文化を保持しながらひしめきあうアジアはユニークで優良な食の宝庫でもある。一方、我々の腸内に宿り第3の臓器とも言われる腸内細菌叢は、食と健康のインターフェースとして近年注目され我々の健康に重要な働きをしていることが分かってきた。そこで九州大学農学研究院微生物工学研究室では、アジア諸国と共同で“Asian Microbiome Project”を展開し、アジア人の食と腸内細菌叢と健康を調査している。その結果、西洋化の中、変わりゆくアジア人の腸内細菌叢の姿が見えてきた。その中で、日本人の腸内細菌叢はどのようになっているのか、そしてそれが我々の健康にどのように関わっているのか解説する。

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  • 農学部 大学院生物資源環境科学府 大学院農学研究院 「新キャンパス」キックオフ!セミナー/九州大学農学研究院における「腸内微生物のメタゲノム解析」研究分野の活動を一般向けに紹介

    九州大学農学研究院  日本橋ライフサイエンスビル  2018年3月

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    対象:社会人・一般, 学術団体, 企業, 市民団体, 行政機関

    種別:セミナー・ワークショップ

  • 農学部 大学院生物資源環境科学府 大学院農学研究院 「新キャンパス」キックオフ!セミナー/九州大学農学研究院における「腸内微生物のメタゲノム解析」研究分野の活動を一般向けに紹介

    九州大学農学研究院  日本橋ライフサイエンスビル  2018年3月

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    種別:セミナー・ワークショップ

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  • 第26回アジア栄養科学ワークショップ「腸内細菌と健康」

    中村学園  中村学園  2018年1月

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    対象:社会人・一般, 学術団体, 企業, 市民団体, 行政機関

    種別:講演会

  • 第26回アジア栄養科学ワークショップ「腸内細菌と健康」

    中村学園  中村学園  2018年1月

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    種別:出前授業

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  • システム生命科学夏の学校

    九州大学システム生命科学府  2016年6月

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    対象:幼稚園以下, 小学生, 中学生, 高校生

    種別:セミナー・ワークショップ

  • 日本農芸化学会出前授業「見えないものの力~わたしたちのくらしと健康を支える微生物の話」

    筑紫女学園中学校  2015年12月

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    対象:幼稚園以下, 小学生, 中学生, 高校生

    種別:セミナー・ワークショップ

  • 日本農芸化学会出前授業「見えないものの力~わたしたちのくらしと健康を支える微生物の話」

    筑紫女学園中学校  2015年12月

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    種別:セミナー・ワークショップ

    researchmap

  • 高校生のための九州大学農学部体験授業プログラム

    2013年8月

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    対象:幼稚園以下, 小学生, 中学生, 高校生

    種別:セミナー・ワークショップ

  • 高校生のための九州大学農学部体験授業プログラム

    2013年8月

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    種別:セミナー・ワークショップ

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  • 出前授業「微生物のパワーとバイオテクノロジー」

    福岡市立福岡西陵高等学校  2013年2月

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    対象:幼稚園以下, 小学生, 中学生, 高校生

    種別:セミナー・ワークショップ

  • 出前授業「微生物のパワーとバイオテクノロジー」

    福岡市立福岡西陵高等学校  2013年2月

     詳細を見る

    種別:セミナー・ワークショップ

    researchmap

  • 微生物学連盟フォーラム『驚きの微生物たち』

    微生物学連盟  東京大学駒場キャンパス  2013年1月

     詳細を見る

    対象:社会人・一般, 学術団体, 企業, 市民団体, 行政機関

    種別:講演会

  • 微生物学連盟フォーラム『驚きの微生物たち』

    微生物学連盟  東京大学駒場キャンパス  2013年1月

     詳細を見る

    種別:出前授業

    researchmap

  • 高校生のための九州大学農学部体験授業プログラム

    2010年8月

     詳細を見る

    対象:幼稚園以下, 小学生, 中学生, 高校生

    種別:セミナー・ワークショップ

  • 高校生のための九州大学農学部体験授業プログラム

    2010年8月

     詳細を見る

    種別:セミナー・ワークショップ

    researchmap

▼全件表示

メディア報道

  • 小雪と発酵おばあちゃん(北九州地方に伝統的に伝わる発酵食品「ぬか漬け」と「ぬか炊き」について、微生物学者として解説) テレビ・ラジオ番組

    NHK(Eテレ)  2023年5月

     詳細を見る

    小雪と発酵おばあちゃん(北九州地方に伝統的に伝わる発酵食品「ぬか漬け」と「ぬか炊き」について、微生物学者として解説)

  • 新窓を開けて九州(本学糠床研究の取材) テレビ・ラジオ番組

    RKB  2021年10月

     詳細を見る

    新窓を開けて九州(本学糠床研究の取材)

  • 人や動物の健康に大きく深くかかわる「おなか」をテーマに、専門家を招いて科学・医療分野の情報を紹介。腸や腸内細菌の話に留まらず、その周辺の話題も取り上げて分かりやすく解説。 テレビ・ラジオ番組

    おなかワンダーランド  2019年10月

     詳細を見る

    人や動物の健康に大きく深くかかわる「おなか」をテーマに、専門家を招いて科学・医療分野の情報を紹介。腸や腸内細菌の話に留まらず、その周辺の話題も取り上げて分かりやすく解説。

外国人研究者等の受け入れ状況

  • University of Teheran

    受入れ期間: 2024年4月 - 2024年7月   (期間):1ヶ月以上

    国籍:イラン・イスラム共和国

  • Chiangmai University

    受入れ期間: 2023年7月 - 2023年10月   (期間):1ヶ月以上

    国籍:タイ王国

  • Chiangmai University

    受入れ期間: 2023年5月 - 2023年7月   (期間):1ヶ月以上

    国籍:タイ王国

  • University of Teheran

    受入れ期間: 2023年1月 - 2023年7月   (期間):1ヶ月以上

    国籍:イラン・イスラム共和国

  • Universitas Gadja Mada

    受入れ期間: 2022年11月   (期間):2週間未満

    国籍:インドネシア共和国

  • Al-Azhar University

    受入れ期間: 2022年6月 - 2022年11月   (期間):1ヶ月以上

    国籍:エジプト・アラブ共和国

  • 受入れ期間: 2020年2月   (期間):2週間未満

    国籍:インドネシア共和国

  • 受入れ期間: 2019年6月  

    国籍:フィリピン共和国

  • フィリピン大学マニラ校

    受入れ期間: 2019年6月   (期間):2週間未満

    国籍:フィリピン共和国

  • ガジャマダ大学

    受入れ期間: 2019年5月   (期間):2週間未満

    国籍:インドネシア共和国

  • Mahidol University

    受入れ期間: 2019年4月 - 2019年5月   (期間):1ヶ月以上

    国籍:タイ王国

  • Al-Azhar University

    受入れ期間: 2018年12月 - 2019年2月   (期間):1ヶ月以上

    国籍:エジプト・アラブ共和国

  • Kasetsart University

    受入れ期間: 2018年2月 - 2018年3月   (期間):1ヶ月以上

    国籍:タイ王国

    専業主体:外国政府・外国研究機関・国際機関

  • Kasetsart University

    受入れ期間: 2016年2月 - 2016年3月   (期間):1ヶ月以上

    国籍:タイ王国

    専業主体:日本学術振興会

  • Al-Azhar University

    受入れ期間: 2015年2月 - 2015年7月   (期間):1ヶ月以上

    国籍:エジプト・アラブ共和国

    専業主体:政府関係機関

  • Kasetsart University

    受入れ期間: 2015年2月 - 2015年3月   (期間):1ヶ月以上

    国籍:タイ王国

    専業主体:日本学術振興会

  • Kasetsart University

    受入れ期間: 2011年2月 - 2011年3月   (期間):1ヶ月以上

    国籍:タイ王国

    専業主体:日本学術振興会

  • King Mongkut Institute Technology Lakkarban

    受入れ期間: 2009年2月 - 2009年3月   (期間):1ヶ月以上

    国籍:タイ王国

    専業主体:日本学術振興会

  • Kasetsart University

    受入れ期間: 2008年3月   (期間):1ヶ月以上

    国籍:タイ王国

    専業主体:日本学術振興会

  • King Mongkut Institute Technology Lakkarban

    受入れ期間: 2008年2月 - 2008年3月   (期間):1ヶ月以上

    国籍:タイ王国

    専業主体:日本学術振興会

  • Kasetsart University

    受入れ期間: 2007年5月 - 2007年6月   (期間):1ヶ月以上

    国籍:タイ王国

    専業主体:日本学術振興会

  • King Mongkut Institute Technology Lakkarban

    受入れ期間: 2007年2月 - 2007年3月   (期間):1ヶ月以上

    国籍:タイ王国

    専業主体:日本学術振興会

  • Kasetsart University

    受入れ期間: 2006年11月 - 2006年12月   (期間):1ヶ月以上

    国籍:タイ王国

    専業主体:日本学術振興会

  • Kasetsart University

    受入れ期間: 2006年10月 - 2006年11月   (期間):1ヶ月以上

    国籍:タイ王国

    専業主体:日本学術振興会

  • Mahidol University

    受入れ期間: 2004年10月 - 2004年11月   (期間):1ヶ月以上

    国籍:タイ王国

    専業主体:日本学術振興会

  • Kasetsart University

    受入れ期間: 2004年10月 - 2004年11月   (期間):1ヶ月以上

    国籍:タイ王国

    専業主体:日本学術振興会

  • Kasetsart University

    受入れ期間: 2004年10月 - 2004年11月   (期間):1ヶ月以上

    国籍:タイ王国

    専業主体:日本学術振興会

  • Kasetsart University

    受入れ期間: 2004年2月 - 2004年4月   (期間):1ヶ月以上

    国籍:タイ王国

    専業主体:日本学術振興会

  • Kasetsart University

    受入れ期間: 2003年11月 - 2003年12月   (期間):1ヶ月以上

    国籍:タイ王国

    専業主体:日本学術振興会

  • Kasetsart Univeristy

    受入れ期間: 2003年11月 - 2003年12月   (期間):1ヶ月以上

    国籍:タイ王国

    専業主体:日本学術振興会

  • Kasetsart University

    受入れ期間: 2002年12月 - 2003年1月   (期間):1ヶ月以上

    国籍:タイ王国

    専業主体:日本学術振興会

  • Kasetsart University

    受入れ期間: 2002年11月 - 2003年1月   (期間):1ヶ月以上

    国籍:タイ王国

    専業主体:日本学術振興会

  • Kasetsart University

    受入れ期間: 2002年3月   (期間):2週間以上1ヶ月未満

    国籍:タイ王国

    専業主体:日本学術振興会

  • Ministry of Agriculture and Cooperatives Kaset-klang

    受入れ期間: 2002年3月   (期間):2週間以上1ヶ月未満

    国籍:タイ王国

    専業主体:日本学術振興会

  • Mahidol University

    受入れ期間: 2002年2月 - 2002年3月   (期間):1ヶ月以上

    国籍:タイ王国

    専業主体:日本学術振興会

  • Tanta University

    受入れ期間: 2001年11月 - 2004年11月   (期間):1ヶ月以上

    国籍:エジプト・アラブ共和国

▼全件表示

海外渡航歴

  • 2023年10月

    滞在国名1:フィリピン共和国   滞在機関名1:ビサヤ大学

  • 2023年8月

    滞在国名1:モンゴル国   滞在機関名1:モンゴル生命科学大学

  • 2023年7月

    滞在国名1:インドネシア共和国   滞在機関名1:ガジャマダ大学

  • 2022年11月

    滞在国名1:インドネシア共和国   滞在機関名1:ウダヤナ大学

  • 2022年8月

    滞在国名1:モンゴル国   滞在機関名1:モンゴル生命科学大学

  • 2022年7月

    滞在国名1:タイ王国   滞在機関名1:マヒドン大学

    滞在機関名2:マエファオラーン大学

    滞在機関名3:カセサート大学

  • 2019年11月

    滞在国名1:タイ王国   滞在機関名1:マヒドン大学

  • 2019年10月

    滞在国名1:インドネシア共和国   滞在機関名1:ガジャマダ大学

  • 2019年7月

    滞在国名1:モンゴル国   滞在機関名1:モンゴル生命科学大学

  • 2019年2月 - 2020年2月

    滞在国名1:タイ王国   滞在機関名1:マヒドン大学

  • 2016年8月

    滞在国名1:シンガポール共和国   滞在機関名1:シンガポール国立大学

  • 2013年1月

    滞在国名1:台湾   滞在機関名1:国立陽明大学

  • 2012年3月

    滞在国名1:タイ王国   滞在機関名1:カセサート大学

  • 2011年11月

    滞在国名1:アメリカ合衆国   滞在機関名1:Massachusetts Eye and Ear Infirmary

  • 2011年11月

    滞在国名1:アメリカ合衆国   滞在機関名1:ミネソタ大学

  • 2011年7月 - 2011年8月

    滞在国名1:グレートブリテン・北アイルランド連合王国(英国)   滞在機関名1:ノッティンガム大学

    滞在国名2:オランダ王国   滞在機関名2:ワーゲニンゲン大学

  • 2011年5月 - 2011年6月

    滞在国名1:日本国   滞在機関名1:東京大学食の安全センター

  • 2008年1月

    滞在国名1:グレートブリテン・北アイルランド連合王国(英国)   滞在機関名1:インペリアルカレッジロンドン

    滞在機関名2:ノッテインガム大学

    滞在機関名3:リーズ大学

  • 2007年1月

    滞在国名1:フランス共和国   滞在機関名1:INRA

  • 1998年4月 - 1999年9月

    滞在国名1:オランダ王国   滞在機関名1:ワーゲニンゲン大学

▼全件表示

学内運営に関わる各種委員・役職等

  • 2024年4月 - 2026年3月   学部 応用生物科学コース長

  • 2024年4月 - 2025年3月   学部 食糧化学工学分野長

  • 2022年4月 - 2025年3月   研究院 熱帯農学研究センター委員会委員

  • 2022年4月 - 2025年3月   研究院 附属国際農業教育・研究推進センター運営委員会委員

  • 2022年4月 - 2024年3月   研究院 国際交流委員会委員

  • 2021年4月 - 2024年3月   研究院 研究教育支援センター副センタ―長

  • 2021年4月 - 2022年3月   専攻 システム生物工学講座長

  • 2019年4月 - 2020年3月   研究院 学生相談員

  • 2018年4月 - 2020年3月   研究院 学務委員

  • 2015年4月 - 2016年3月   全学 環境安全衛生推進室エネルギー資源管理部門

  • 2013年4月 - 2021年3月   センター 研究教育支援センター運営委員会

  • 2013年4月 - 2021年3月   センター 環境発達医学研究センター運営委員会

  • 2013年4月 - 2021年3月   センター 中央分析センター伊都分室委員

  • 2012年4月 - 2016年3月   学部 国際交流委員会

  • 2012年4月 - 2015年3月   学部 サバチカル制度運用委員会

  • 2012年4月 - 2014年3月   学部 農学研究院新キャンパス委員会委員

  • 2010年4月 - 2019年3月   学部 農学研究院研究教育支援センター運営委員会委員

  • 2010年4月 - 2011年3月   学部 農学研究院新キャンパス委員会委員

  • 2003年4月 - 2005年3月   学府 学務委員

  • その他 国際交流委員会委員

  • その他 熱帯農学研究センター委員会委員

  • その他 農学研究院研究教育支援センター生物資源環境科学部門長

▼全件表示