Updated on 2024/08/06

Information

 

写真a

 
KAZAMA TOMOHIKO
 
Organization
Faculty of Agriculture Department of Bioscience and Biotechnology Associate Professor
Plant Frontier Research Center （Joint Appointment）
School of Agriculture Department of Bioresource and Bioenvironment（Joint Appointment）
Graduate School of Bioresource and Bioenvironmental Sciences Department of Bioscience and Biotechnology（Joint Appointment）
Title
Associate Professor
Contact information
メールアドレス
Profile
Our aims are understanding of structure and functions of genome, and utilization of the knowledge into genome engineering technologies. Especially, we have focused on cooperative regulation between nuclear genome and genomes of symbiotic organelles, such as mitochondria and chloroplasts, in plants, by using biochemical, genetic, and molecular biological approaches. Pentatricopeptide repeat (PPR) protein is one of such regulators, which is nuclear-encoded, sequence-specific RNA binding protein and involved in organelle gene expression. We have elucidated the RNA recognition rule of PPR protein, and have started the development of a new genome-editing tool.
External link

Research Areas

  • Environmental Science/Agriculture Science / Science in plant genetics and breeding

Degree

  • Ph.D.

Research History

  • 東北大学（助教）

Research Interests・Research Keywords

  • Research theme：Molecular genetic research on cytoplasmic male sterile rice

    Keyword：male sterility

    Research period： 2021.3 - 2025.5

  • Research theme：Genetic engineering of plant mitochondrial genome

    Keyword：mitochondria

    Research period： 2019.10 - 2020.6

Awards

  • 技術賞

    2021.9   日本植物バイオテクノロジー学会   植物ミトコンドリアゲノム編集技術の開発と細胞質雄性不稔原因遺伝子の同定

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    これまで遺伝子改変が不可能であった植物ミトコンドリアゲノムについて、ゲノム編集（標的遺伝子破壊）に世界で初めて成功しました。また開発した方法を用いて、イネとナタネのミトコンドリアゲノム上の細胞質雄性不稔（CMS）関連遺伝子を破壊することで、実際に稔性回復が起こることを最終証明しました。CMSは農業上の重要形質ですが、原因遺伝子は種や品種で異なり、今後様々な作物での遺伝子同定や解析、創出に大きな貢献が期待されます。

  • 日本育種学会奨励賞

    2017.3   一般社団法人日本育種学会  

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    細胞質雄性不稔イネの稔性回復メカニズムの分子遺伝学的解析における優れた業績

  • 平成27年度先端農学研究奨励賞

    2015.9   東北大学大学院農学研究科  

  • 第118回講演会日本育種学会優秀発表賞

    2010.11   日本育種学会   優秀発表賞

Papers

  • Disruption of mitochondrial open reading frame 352 partially restores pollen development in cytoplasmic male sterile rice Reviewed International journal

    Omukai, S., Arimura, S. I., Toriyama, K. & Kazama, T.,

    PLANT PHYSIOLOGY   187 ( 1 )   236 - 246   2021.9

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1093/plphys/kiab236

  • Prospects for organelle genome breeding

    Kuwabara Kosuke, Tsujimura Mai, Nakazato Issei, Tsutsumi Nobuhiro, Takenaka Mizuki, Kazama Tomohiko

    Breeding Research   26 ( 1 )   61 - 66   2024.6   （ ISSN:13447629 eISSN:13481290 ）

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    Language：Japanese   Publishing type：Research paper (scientific journal)   Publisher：Japanese Society of Breeding  

    DOI： 10.1270/jsbbr.26.w02

    CiNii Research

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  • Translational enhancement of target endogenous mRNA in mammalian cells using programmable RNA-binding pentatricopeptide repeat proteins

    Ping, N; Hara-Kuge, S; Yagi, Y; Kazama, T; Nakamura, T

    SCIENTIFIC REPORTS   14 ( 1 )   251   2024.1   （ ISSN:2045-2322 ）

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    Language：English   Publisher：Scientific Reports  

    Programmable protein scaffolds are invaluable in the development of genome engineering tools. The pentatricopeptide repeat (PPR) protein is an attractive platform for RNA manipulation because of its programmable RNA-binding selectivity, which is determined by the combination of amino acid species at three specific sites in the PPR motif. Translation is a key RNA regulatory step that determines the final gene expression level and is involved in various human diseases. In this study, designer PPR protein was used to develop a translational enhancement technique by fusion with the translation initiation factor eIF4G. The results showed that the PPR-eIF4G fusion protein could activate the translation of endogenous c-Myc and p53 mRNAs and control cell fate, indicating that PPR-based translational enhancement is a versatile technique applicable to various endogenous mRNAs in mammalian cells. In addition, the translational enhancement was dependent on both the target position and presence of eIF4G, suggesting the presence of an unknown translation activation mechanism.

    DOI： 10.1038/s41598-023-50776-z

    Web of Science

    Scopus

    PubMed

  • The mitochondrial and plastid genomes of Oryza sativa L. cv. Taichung 65 Reviewed International journal

    Hiroyuki Ichida, Tomohiko Kazama, Shin Ichi Arimura, Kinya Toriyama

    Plant Biotechnology   40 ( 1 )   109 - 112   2023.2   （ ISSN:13424580 eISSN:13476114 ）

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：Japanese Society for Plant Biotechnology  

    <p>A highly contiguous mitochondrial and plastid genome sequences of a <i>japonica</i> rice cultivar, Taichung 65, were determined by a hybrid approach with long- and short-read sequences. The assembled mitochondrial genome was 465,453 bases in length with an overall GC content of 43.8%. It was predicted to harbor 62 protein-encoding genes, 16 kinds (33 copies) of transfer RNA, and three kinds (six copies) of ribosomal RNA genes. The mitochondrial genome structure in Taichung 65 is largely the same as that of Nipponbare, but the first ∼9.5 kb sequence in Nipponbare (DQ167400) is replaced with a ∼27 kb sequence duplicated from other parts of the mitochondrial genome. Phylogenetic and sequence polymorphism analysis indicated that Taichung 65 is classified as typical <i>japonica</i>. The assembled plastid genome sequence was 134,551 bases in length and completely identical to the previously reported Nipponbare sequence. These near-complete organelle genome sequences will serve as fundamental resources for investigating alloplasmic cytoplasmic male sterile lines and other organelle-controlled phenomena in rice.</p>

    DOI： 10.5511/plantbiotechnology.22.1213a

    Web of Science

    PubMed

    CiNii Research

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  • A Method for Precisely Identifying Modifications to Plant Mitochondrial Genomes by mitoTALENs Invited Reviewed International journal

    Tomohiko Kazama, Shin ichi Arimura

    Methods in Molecular Biology   2615   365 - 378   2023.2

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： doi.org/10.1007/978-1-0716-2922-2_25

  • TALEN-mediated depletion of the mitochondrial gene orf312 proves that it is a Tadukan-type cytoplasmic male sterility-causative gene in rice Reviewed International journal

    Takatsuka, A., Kazama, T., Arimura, S. I. & Toriyama, K.

    PLANT JOURNAL   110 ( 4 )   994 - 1004   2022.5   （ ISSN:0960-7412 eISSN:1365-313X ）

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    Language：Japanese   Publishing type：Research paper (scientific journal)   Publisher：WILEY  

    Cytoplasmic male sterility (CMS) is a trait that causes pollen or anther dysfunctions, resulting in the lack of seed setting. CMS is considered to be caused by the expression of a unique mitochondrial open reading frame referred to as CMS-associated gene. orf312 has been reported as a CMS-associated gene of Tadukan-type CMS (TAA) in rice (Oryza sativa L.), which exhibits impaired anther dehiscence; however, evidence thereof has not yet been reported. Here, we took a loss-of-function approach, using a mitochondria-targeted transcription activator-like effector nuclease (mitoTALEN) designed to knock out orf312 in TAA, to prove that orf312 indeed is a CMS-causative gene. Out of 28 transgenic TAA plants harboring the mitoTALEN expression vector, deletion of orf312 was detected in 24 plants by PCR, Southern blot, and sequencing analyses. The 24 plants were grouped into three groups based on the deleted regions. All orf312-depleted TAA plants exhibited recovery of anther dehiscence and seed setting. The depletion of orf312 and fertility restoration was maintained in the next generation, even in mitoTALEN expression cassette null segregants. In contrast, orf312-retaining plants were sterile. These results provide robust evidence that orf312 is a Tadukan-type CMS-causative gene.

    DOI： 10.1111/tpj.15715

    Web of Science

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  • Cytoplasmic Male Sterility-Associated Mitochondrial Gene orf312 Derived from Rice (Oryza sativa L.) Cultivar Tadukan Reviewed International journal

    @Ayumu Takatsuka, Tomohiko Kazama, @Kinya Toriyama

    Rice   2021.5

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    Language：Japanese   Publishing type：Research paper (scientific journal)  

    DOI： doi.org/10.1186/s12284-021-00488-7

  • Fertility restoation of Chinese wild rice-type cytoplasmic male sterility by CRISPR/Cas9-mediated genome editing of nuclear-encoded RETROGRADE-REGULATED MALE STERILITY. Reviewed International journal

    @Suketomo C., Kazama T., @Toriyama K.

    Plant Biotechnology   2020.9

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    Language：English   Publishing type：Research paper (scientific journal)  

  • Evaluation of rice grain yield and yield components of Nona Bokra chromosome segment substitution lines with the genetic background of Koshihikari, in a saline paddy field Reviewed International journal

    Mitsuya, S., Murakami, N., Sato, T., Kazama, T., Toriyama, K., Skoulding, N. S., Kano-Nakata, M., Yamauchi, A. & Ismail, A. M.

    AoB PLANTS   2019.10

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    Language：Japanese   Publishing type：Research paper (scientific journal)  

  • LONG GRAIN 1: a novel gene that regulates grain length in rice Reviewed

    Morita Ryouhei, Ichida Hiroyuki, Ishii Kotaro, Hayashi Yoriko, Abe Hiroshi, Shirakawa Yuki, Ichinose Katsunori, Tsuneizumi Kazuhide, Kazama Tomohiko, Toriyama Kinya, Sato Tadashi, Abe Tomoko

    MOLECULAR BREEDING   39 ( 9 )   2019.9

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    Language：Others  

    LONG GRAIN 1: a novel gene that regulates grain length in rice

    DOI： 10.1007/s11032-019-1032-1

  • Development of cytoplasmic male sterile lines and restorer lines of various elite Indica Group rice cultivars using CW-CMS/Rf17 system. Reviewed

    Toriyama K, Kazama T, Sato T, Fukuta Y, Oka M

    Rice (New York, N.Y.)   12 ( 1 )   73   2019.9

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    Language：Others  

    Development of cytoplasmic male sterile lines and restorer lines of various elite Indica Group rice cultivars using CW-CMS/Rf17 system.

    DOI： 10.1186/s12284-019-0332-8

  • Curing cytoplasmic male sterility via TALEN-mediated mitochondrial genome editing. Reviewed

    Kazama T, Okuno M, Watari Y, Yanase S, Koizuka C, Tsuruta Y, Sugaya H, Toyoda A, Itoh T, Tsutsumi N, Toriyama K, Koizuka N, Arimura SI

    Nature plants   5 ( 7 )   722 - 730   2019.7

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    Language：English   Publishing type：Research paper (scientific journal)  

    Curing cytoplasmic male sterility via TALEN-mediated mitochondrial genome editing.
    Sequence-specific nucleases are commonly used to modify the nuclear genome of plants. However, targeted modification of the mitochondria! genome of land plants has not yet been achieved. In plants, a type of male sterility called cytoplasmic male sterility (CMS) has been attributed to certain mitochondria! genes, but none of these genes has been validated by direct mitochondria! gene-targeted modification. Here, we knocked out CMS-associated genes (orf79 and orf125) of CMS varieties of rice and rapeseed, respectively, using transcription activator-like effector nucleases (TALENs) with mitochondria localization signals (mitoTALENs). We demonstrate that knocking out these genes cures male sterility, strongly suggesting that these genes are causes of CMS. Sequencing revealed that double-strand breaks induced by mitoTALENs were repaired by homologous recombination, and that during this process, the target genes and surrounding sequences were deleted. Our results show that mitoTALENs can be used to stably and heritably modify the mitochondria! genome in plants.

    DOI： 10.1038/s41477-019-0459-z

  • Genetic analysis of male sterility obtained from a rice cultivar Lebed backcrossed with Taichung 65 Reviewed

    Tetsuya Murakami, Tomohiko Kazama, Kinya Toriyama

    Rice   11 ( 1 )   2018.12

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1186/s12284-018-0222-5

  • A Gene Encoding Pentatricopeptide Repeat Protein Partially Restores Fertility in RT98-Type Cytoplasmic Male-Sterile Rice Reviewed

    Keisuke Igarashi, Tomohiko Kazama, Kinya Toriyama

    PLANT AND CELL PHYSIOLOGY   57 ( 10 )   2187 - 2193   2016.10

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1093/pcp/pcw135

  • Whole Mitochondrial Genome Sequencing and Re-Examination of a Cytoplasmic Male Sterility-Associated Gene in Boro-Taichung-Type Cytoplasmic Male Sterile Rice Reviewed

    Tomohiko Kazama, Kinya Toriyama

    PLOS ONE   11 ( 7 )   2016.7

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1371/journal.pone.0159379

  • Development of cytoplasmic male sterile IR24 and IR64 using CW-CMS/Rf17 system Reviewed

    Toriyama* K, Kazama T

    Rice   9 ( 22 )   1 - 4   2016.5

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    Language：English   Publishing type：Research paper (scientific journal)  

    Development of cytoplasmic male sterile IR24 and IR64 using CW-CMS/Rf17 system

    DOI： 10.1186/s12284-016-0097-2

  • Mitochondrial ORF79 levels determine pollen abortion in cytoplasmic male sterile rice Reviewed

    Tomohiko Kazama, Etsuko Itabashi, Shinya Fujii, Takahiro Nakamura, Kinya Toriyama

    PLANT JOURNAL   85 ( 6 )   707 - 716   2016.3

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1111/tpj.13135

  • AEF1/MPR25 is implicated in RNA editing of plastid atpF and mitochondrial nad5, and also promotes atpF splicing in Arabidopsis and rice Reviewed

    Aaron Yap, Peter Kindgren, Catherine Colas des Francs-Small, Tomohiko Kazama, Sandra K. Tanz, Kinya Toriyama, Ian Small

    PLANT JOURNAL   81 ( 5 )   661 - 669   2015.3

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1111/tpj.12756

  • A candidate factor that interacts with RF2, a restorer of fertility of Lead rice-type cytoplasmic male sterility in rice Reviewed

    Shinya Fujii, Tomohiko Kazama, Yukihiro Ito, Soichi Kojima, Kinya Toriyama

    RICE   7   21   2014.10

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1186/s12284-014-0021-6

  • Heterogeneity of the 5 '-end in plant mRNA may be involved in mitochondrial translation Invited Reviewed

    Tomohiko Kazama, Yusuke Yagi, Kinya Toriyama, Takahiro Nakamura

    FRONTIERS IN PLANT SCIENCE   4   517   2013.12

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.3389/fpls.2013.00517

  • Whole mitochondrial genome sequencing and transcriptional analysis to uncover an RT102-Type cytoplasmic male sterility-associated candidate gene derived from oryza rufipogon Reviewed

    Masayuki Okazaki, Tomohiko Kazama, Hayato Murata, Keiji Motomura, Kinya Toriyama

    Plant and Cell Physiology   54 ( 9 )   1560 - 1568   2013.9

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1093/pcp/pct102

  • Whole Genomic Sequencing of RT98 Mitochondria Derived from Oryza rufipogon and Northern Blot Analysis to Uncover a Cytoplasmic Male Sterility-Associated Gene Reviewed

    Keisuke Igarashi, Tomohiko Kazama, Keiji Motomura, Kinya Toriyama

    Plant and Cell Physiology   54 ( 2 )   237 - 243   2013.2

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1093/pcp/pcs177

  • Multiple Mechanisms and Challenges for the Application of Allopolyploidy in Plants Invited Reviewed

    Kenji Osabe, Takahiro Kawanabe, Taku Sasaki, Ryo Ishikawa, Keiichi Okazaki, Elizabeth S. Dennis, Tomohiko Kazama, Ryo Fujimoto

    INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES   13 ( 7 )   8696 - 8721   2012.7

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.3390/ijms13078696

  • Rice MPR25 encodes a pentatricopeptide repeat protein and is essential for RNA editing of nad5 transcripts in mitochondria Reviewed

    Toda T, Fujii S, Noguchi K, Kazama T, Toriyama K

    Plant J.   2012.7

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    Language：English   Publishing type：Research paper (scientific journal)  

    Rice MPR25 encodes a pentatricopeptide repeat protein and is essential for RNA editing of nad5 transcripts in mitochondria

    DOI： 10.1111/j.1365-313X.2012.05091.x.

  • Identification and characterization of the RNA binding surface of the pentatricopeptide repeat protein Reviewed

    Keiko Kobayashi, Masuyo Kawabata, Keizo Hisano, Tomohiko Kazama, Ken Matsuoka, Mamoru Sugita, Takahiro Nakamura

    NUCLEIC ACIDS RESEARCH   40 ( 6 )   2712 - 2723   2012.3

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1093/nar/gkr1084

  • The fertility restorer gene, Rf2, for Lead Rice-type cytoplasmic male sterility of rice encodes a mitochondrial glycine-rich protein Reviewed

    Etsuko Itabashi, Natsuko Iwata, Sota Fujii, Tomohiko Kazama, Kinya Toriyama

    PLANT JOURNAL   65 ( 3 )   359 - 367   2011.2

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1111/j.1365-313X.2010.04427.x

  • Discovery of global genomic re-organization based on comparison of two newly sequenced rice mitochondrial genomes with cytoplasmic male sterility-related genes Reviewed

    Sota Fujii, Tomohiko Kazama, Mari Yamada, Kinya Toriyama

    BMC GENOMICS   11   209 - 224   2010.3

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1186/1471-2164-11-209

  • Moleculae analysis of CW-type cytoplasmic male sterility and Rf17-mediated fertility restoration for hybeid rice breeding. Reviewed

    Toriyama K, Fujii S, Toda T, Itabashi E, Yamada M, Kazama T

    SARAO Journal of Breeding and Genetics, Vol. 41, Special Supplement August 2009 ISSN 1029-7073 CD   2009.8

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    Language：English   Publishing type：Research paper (other academic)  

    Moleculae analysis of CW-type cytoplasmic male sterility and Rf17-mediated fertility restoration for hybeid rice breeding.
    Proceeding of The 14th Australian Plant Breeding Conference and 11th Society for the Advancement of Breeding Research in Asia and Oceania (SABRAO) Congress 2009

  • Characterization of cytoplasmic male sterility of rice with Lead Rice cytoplasm in comparison with that with Chinsurah Boro II cytoplasm Reviewed

    Etsuko Itabashi, Tomohiko Kazama, Kinya Toriyama

    PLANT CELL REPORTS   28 ( 2 )   233 - 239   2009.2

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1007/s00299-008-0625-7

  • Various spatiotemporal expression profiles of anther-expressed genes in rice. Reviewed

    Tokunori Hobo, Keita Suwabe, Koichiro Aya, Go Suzuki, Kentaro Yano, Takeshi Ishimizu, Masahiro Fujita, Shunsuke Kikuchi, Kazuki Hamada, Masumi Miyano, Tomoaki Fujioka, Fumi Kaneko, Tomohiko Kazama, Yoko Mizuta, Hirokazu Takahashi, Katsuhiro Shiono, Mikio Nakazono, Nobuhiro Tsutsumi, Yoshiaki Nagamura, Nori Kurata, Masao Watanabe, Makoto Matsuoka

    Plant & cell physiology   49 ( 10 )   1417 - 28   2008.10

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1093/pcp/pcn128

  • Separated transcriptomes of male gametophyte and tapetum in rice: validity of a laser microdissection (LM) microarray. Invited Reviewed

    Keita Suwabe, Go Suzuki, Hirokazu Takahashi, Katsuhiro Shiono, Makoto Endo, Kentaro Yano, Masahiro Fujita, Hiromi Masuko, Hiroshi Saito, Tomoaki Fujioka, Fumi Kaneko, Tomohiko Kazama, Yoko Mizuta, Makiko Kawagishi-Kobayashi, Nobuhiro Tsutsumi, Nori Kurata, Mikio Nakazono, Masao Watanabe

    Plant & cell physiology   49 ( 10 )   1407 - 16   2008.10

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1093/pcp/pcn124

  • Suppression mechanism of mitochondrial ORF79 accumulation by Rf1 protein in BT-type cytoplasmic male sterile rice Invited Reviewed

    Tomohiko Kazama, Takahiro Nakamura, Masao Watanabe, Mamoru Sugita, Kinya Toriyama

    PLANT JOURNAL   55 ( 4 )   619 - 628   2008.8

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1111/j.1365-313X.2008.03529.x

  • Molecular characterization of two anther-specific genes encoding putative RNA-binding proteins, AtRBP45s, in Arabidopsis thaliana Reviewed

    Jong-In Park, Makoto Endo, Tomohiko Kazama, Hiroshi Saito, Hirokazu Hakozaki, Yoshinobu Takada, Makiko Kawagishi-Kobayashi, Masao Watanabe

    GENES & GENETIC SYSTEMS   81 ( 5 )   355 - 359   2006.10

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1266/ggs.81.355

  • A pentatricopeptide repeat-containing gene that promotes the processing of aberrant atp6 RNA of cytoplasmic male-sterile rice Reviewed

    T Kazama, K Toriyama

    FEBS LETTERS   544 ( 1-3 )   99 - 102   2003.6

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/S0014-5793(03)00480-0

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Books

  • さまざまなモデル生物からミトコンドリア画分を分離する簡便な方法

    田中敦, 岡本徳子, 風間智彦（ Role： Joint author）

    羊土社  2014.9 

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    Language：Japanese  

Presentations

  • 細胞質雄性不稔性植物をモデルとしたミトコンドリアゲノム編集 Invited

    風間智彦・肥塚信也・有村慎一

    日本育種学会第136回講演会  2019.9 

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    Event date： 2019.9

    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：近畿大学（奈良）   Country：Japan  

  • ミトコンドリア移行TALENを用いたCMS原因遺伝子候補 orf352 のノックアウト

    大向詩穂・鳥山 欽哉・有村 慎一・風間 智彦

    日本育種学会第136回講演会  2019.9 

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    Event date： 2019.9

    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：近畿大学（奈良）   Country：Japan  

  • mitoTALEN によるミトコンドリアゲノム編集: BT型細胞質雄性不稔性イネにおけるミトコンドリア遺伝子 orf79 の破壊

    風間智彦・亘悠太・堤伸浩・鳥山欽哉・有村慎一

    日本育種学会第135回講演会  2019.3 

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    Event date： 2019.3

    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：千葉大学（千葉）   Country：Japan  

  • Targeted mitochondrial gene editing to reveal cytoplasmic Invited

    Tomohiko Kazama

    2024.3 

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    Event date： 2024.3

    Language：English   Presentation type：Symposium, workshop panel (public)  

    Country：Japan  

  • Mitochondra-targeted TALEN-mediated gene knockout and reorganization of mitochondrial genome in Tadukan-type cytoplasmic male sterile rice Invited

    Ayumu Takatsuka, Tomohiko Kazama, Shin-ichi Arimura, Kinya Toriyama

    2024.3 

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    Event date： 2024.3

    Language：English   Presentation type：Symposium, workshop panel (public)  

    Country：Japan  

  • 植物ミトコンドリアが引き起こすオス殺し現象 Invited

    風間 智彦

    九州沖縄植物学会  2023.12 

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    Event date： 2023.12

    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：九州大学   Country：Japan  

  • ミトコンドリアゲノム編集を用いた細胞質雄性不稔性原因遺伝子の同定

    風間 智彦，鳥山 欽哉，有村 慎一

    日本ミトコンドリア学会  2023.11 

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    Event date： 2023.11

    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：つくば国際会議場   Country：Japan  

  • Mitochondrial gene knockout and mechanistic analysis of Tadukan-type cytoplasmic male sterile rice International conference

    Ayumu Takatsuka, Tomohiko Kazama, Shin-ichi Arimura, Kinya Toriyama

    6th International Rice Congress 2023/8th International hybrid rice symposium  2023.10 

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    Event date： 2023.10

    Language：English   Presentation type：Oral presentation (general)  

    Country：Philippines  

  • A proposed model for the molecular mechanism of Tadukan-type cytoplasmic male sterility based on morphological observation and transcriptomic analysis

    2023.9 

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    Event date： 2023.9

    Language：Japanese  

    Country：Japan  

  • 細胞質雄性不稔性イネを用いたミトコンドリアゲノム編集の現状と今後の課題

    風間智彦

    日本育種学会144回講演会  2023.9 

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    Event date： 2023.9

    Language：Japanese   Presentation type：Symposium, workshop panel (public)  

    Venue：神戸大学   Country：Japan  

  • イネ品種Tadukanに由来する細胞質雄性不稔性イネの形態観察およびトランスクリプトーム解析

    高塚歩、風間智彦、鳥山欽哉

    第40回日本植物バイオテクノロジー学会 (千葉) 大会  2023.9 

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    Event date： 2023.9

    Language：Japanese  

    Venue：千葉大学   Country：Japan  

  • MitoTALEN-mediated mitochondrial gene knockout and mechanistic analysis of Tadukan-type cytoplasmic male sterile rice International conference

    Ayumu Takatsuka, Tomohiko Kazama, Shin-ichi Arimura, Kinya Toriyama

    6th Plants and People Conference  2023.9 

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    Event date： 2023.9

    Language：English   Presentation type：Oral presentation (general)  

    Country：Germany  

  • MitoTALEN-mediated mitochondrial gene knockout and mechanistic analysis of Tadukan-type cytoplasmic male sterile rice International conference

    Ayumu Takatsuka, Tomohiko Kazama, Shin-ichi Arimura, Kinya Toriyama

    Core to core Minisymposium  2023.9 

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    Event date： 2023.9

    Language：English   Presentation type：Oral presentation (general)  

    Country：Germany  

  • Targeted knockou of CMS-associated mitochodnrial genes in rice International conference

    Tomohiko Kazama

    JSPS core to core meeting  2023.9 

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    Event date： 2023.9

    Language：English   Presentation type：Oral presentation (general)  

    Country：France  

  • MitoTALEN-mediated mitochondrial gene knockout and mechanistic analysis of Tadukan-type cytoplasmic male sterile rice International conference

    Ayumu Takatsuka, Tomohiko Kazama, Shin-ichi Arimura, Kinya Toriyama

    JSPS core to core meeting  2023.9 

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    Event date： 2023.9

    Language：English   Presentation type：Oral presentation (general)  

    Country：France  

  • MitoTALEN-mediated mitochondrial gene knockout and mechanistic analysis of Tadukan-type cytoplasmic male sterile rice International conference

    Ayumu Takatsuka, Tomohiko Kazama, Shin-ichi Arimura, Kinya Toriyama

    IBMP JSPS core to core meeting  2023.8 

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    Event date： 2023.8

    Language：English   Presentation type：Oral presentation (general)  

    Country：France  

  • Targeted knockou of CMS-associated mitochodnrial genes in rice International conference

    Tomohiko Kazama

    IBMP JSPS core to core meeting  2023.8 

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    Event date： 2023.8

    Language：English   Presentation type：Oral presentation (general)  

    Country：France  

  • Identification of a mitochondrial gene causing Tadukan-type cytoplasmic male sterility by the mitochondrial genome reorganization mediated by TALEN International conference

    Ayumu Takatsuka, Tomohiko Kazama, Shin-ichi Arimura, Kinya Toriyama

    International Association for Plant Biotechnology Congress  2023.8 

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    Event date： 2023.8

    Language：English  

    Country：Korea, Republic of  

  • インディカイネTadukanに由来するミトコンドリア遺伝子が葯の裂開を阻害するメカニズムの解明

    高塚歩、風間智彦、鳥山欽哉

    イネ遺伝学・分子生物学ワークショップ2023  2023.7 

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    Event date： 2023.7

    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：九州大学   Country：Japan  

  • ミトコンドリア遺伝子orf312が葯の裂開を阻害するTadukan型細胞質雄性不稔性イネの組織学的解析

    @高塚歩、風間智彦、@鳥山欽哉

    第64回日本植物生理学会年会  2023.3 

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    Event date： 2023.3

    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：東北大学　川内キャンパス   Country：Japan  

  • mitoTALENの利用で明らかとなった細胞質雄性不稔イネの原因遺伝子 Invited

    風間智彦

    中国育種談話会  2022.12 

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    Event date： 2022.12

    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：山口大学   Country：Japan  

  • イネのミトコンドリア遺伝性の雄性生殖器官発育不全と核のレスキュー遺伝子

    @高塚歩、風間智彦、@有村慎一、@市田裕之、@阿部知子、@鳥山欽哉

    第45回⽇本分⼦⽣物学会年会  2022.11 

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    Event date： 2022.11 - 2022.12

    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：幕張メッセ   Country：Japan  

  • Exploration for a restorer of fertility gene for cytoplasmic male sterility derived from Oryza sativa cv. Tadukan

    Ayumu Takatsuka, Tomohiko Kazama, Hiroyuki Ichida, Tomoko Abe, Kinya Toriyama

    2022.11 

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    Event date： 2022.11

    Language：Japanese   Presentation type：Oral presentation (general)  

    Country：Japan  

  • TALEN-mediated reorganization of the rice mitochondrial genome deleting a Tadukan-type cytoplasmic male sterility-causing gene International conference

    Ayumu Takatsuka, Tomohiko Kazama, Shin-ichi Arimura, Kinya Toriyama

    19th International Symposium on Rice Functional Genomics  2022.11 

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    Event date： 2022.11

    Language：English   Presentation type：Oral presentation (general)  

    Country：Thailand  

  • Tadukan型細胞質雄性不稔性イネを稔性回復させる遺伝子の探索

    @高塚歩、風間智彦、@市田裕之、@阿部知子、@鳥山欽哉

    日本育種学会第142回講演会  2022.9 

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    Event date： 2022.9

    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：帯広畜産大学   Country：Japan  

  • Tadukanに由来する細胞質雄性不稔性イネの稔性回復候補遺伝子

    @高塚歩、風間智彦、@市田裕之、@阿部知子、@鳥山欽哉

    第39回植物バイオテクノロジー学会(堺)大会  2022.9 

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    Event date： 2022.9

    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：大阪公立大学 中百舌鳥キャンパス   Country：Japan  

  • イネの雄性生殖器官の発育不全を引き起こすミトコンドリア遺伝子のゲノム編集

    @高塚歩、風間智彦、@有村慎一、@鳥山欽哉

    日本生化学会 東北支部 第88回例会・シンポジウム  2022.5 

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    Event date： 2022.5

    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：鶴岡市先端研究産業支援センター   Country：Japan  

  • Tadukan型細胞質雄性不稔性イネの花粉発達は正常だが葯の裂開が阻害され稔性回復は胞子体型的に行われる

    髙塚歩、風間智彦、鳥山欽哉

    日本育種学会第141回  2022.3 

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    Event date： 2022.3

    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：オンライン   Country：Japan  

  • イネ品種Tadukanに由来するミトコンドリア雄性不稔遺伝子のmitoTALENによるノックアウトと後代の安定性

    髙塚歩、風間智彦、有村慎一、鳥山欽哉

    第44回　日本分子生物学会  2021.12 

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    Event date： 2021.12

    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：オンライン   Country：Japan  

  • 台中65号の細胞質を持つOryza glaberrimaのCMS関連遺伝子の解析

    武田信哉、市田裕之、阿部知子、有村慎一、風間智彦、陳孫禄、金岡義高、貴島祐治、鳥山欽哉

    日本育種学会第140回講演会  2021.9 

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    Event date： 2021.9

    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：オンライン   Country：Japan  

  • Oryza nivaraに由来する細胞質を持つ細胞質雄性不稔イネに関する分子遺伝学的解析

    浅沼沙弥果、鳥山欽哉、濱岡範光、山形悦透、安井秀、風間智彦

    日本育種学会第140回講演会  2021.9 

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    Event date： 2021.9

    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：オンライン   Country：Japan  

  • イネTA-CMSにおいてミトコンドリア移行TALENが引き起こす相同組換え修復およびゲノム構成の変化の解析

    髙塚歩、風間智彦、有村慎一、鳥山欽哉

    日本育種学会第140回講演会  2021.9 

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    Event date： 2021.9

    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：オンライン   Country：Japan  

  • mitoTALEN導入によるTadukan型細胞質雄性不稔性イネにおけるゲノム再構成の誘発

    髙塚歩、風間智彦、有村慎一、鳥山欽哉

    第38回　植物バイオテクノロジー学会（つくば）大会  2021.9 

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    Event date： 2021.9

    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：オンライン   Country：Japan  

  • 植物ミトコンドリアゲノム編集技術の開発と細胞質雄性不稔原因遺伝子の同定

    風間智彦、肥塚信也、鳥山欽哉、堤伸浩、有村慎一

    第38回　植物バイオテクノロジー学会（つくば）大会  2021.9 

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    Event date： 2021.9

    Language：Japanese   Presentation type：Oral presentation (invited, special)  

    Venue：オンライン   Country：Japan  

  • ミトコンドリア移行TALENによるCW型細胞質雄性不稔イネの原因遺伝子の同定

    木下諄美、有村慎一、鳥山欽哉、風間智彦

    第38回　植物バイオテクノロジー学会（つくば）大会  2021.9 

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    Event date： 2021.9

    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：オンライン   Country：Japan  

  • ミトコンドリア移行TALEN を用いたイネ品種Tadukan における雄性不稔遺伝子の機能証明

    髙塚歩、風間智彦、有村慎一、鳥山欽哉

    日本ゲノム編集学会第6回大会  2021.6 

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    Event date： 2021.6

    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：オンライン   Country：Japan  

  • mitoTALENを用いたミトコンドリア遺伝子破壊によるイネTA型CMS原因遺伝子の同定

    髙塚歩、風間智彦、有村慎一、鳥山欽哉

    日本育種学会第139回講演会  2021.3 

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    Event date： 2021.3

    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：オンライン   Country：Japan  

  • MitoTALEN-mediated mitochondrial gene-knockout revealed a cytoplasmic male sterility-causative gene in Oryza sativa cv. Tadukan. International conference

    Ayumu Takatsuka, Tomohiko Kazama, Shin-ichi Arimura, Kinya Toriyama

    KEYSTONE SYMPOSIA Plant Genome Engineering:From lab to Field  2021.3 

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    Event date： 2021.3

    Language：English   Presentation type：Oral presentation (general)  

    Country：Japan  

  • イネ品種Lebedに台中65号を戻し交雑して得られた雄性不稔系統における原因候補遺伝子の機能推定

    藤田悠生、市田裕之、風間智彦、阿部知子、鳥山欽哉

    第15回東北育種研究集会  2020.12 

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    Event date： 2020.12

    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：福島大学（オンライン）   Country：Japan  

  • イネTadukan型細胞質雄性不稔性のミトコンドリア原因遺伝子orf312の同定

    @髙塚歩、風間智彦、@有村慎一、@鳥山欽哉

    第15回東北育種研究集会  2020.12 

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    Event date： 2020.12

    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：福島大学（オンライン）   Country：Japan  

  • orf352を持つRT102型およびWA型細胞質雄性不稔性イネの分子遺伝学的比較

    大向志穂、鳥山欽哉、有村慎一、風間智彦

    第15回東北育種研究集会  2020.12 

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    Event date： 2020.12

    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：福島大学（オンライン）   Country：Japan  

  • RT102型細胞質雄性不稔性におけるミトコンドリア移行TALENを用いたorf352の消失は花粉発達を一部回復させる

    大向志穂、鳥山欽哉、有村慎一、風間智彦

    第43回日本分子生物学会年会  2020.12 

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    Event date： 2020.12

    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：オンライン開催   Country：Japan  

  • インディカ品種Tadukanの細胞質に由来する雄性不稔性原因候補遺伝子の探索

    高塚歩、風間智彦、鳥山欽哉

    日本育種学会第137回講演会  2020.10 

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    Event date： 2020.10

    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：オンライン   Country：Japan  

  • RT102型細胞質雄性不稔イネのミトコンドリアゲノム編集によって生じた新規orfの発現解析

    大向志穂、鳥山欽哉、有村慎一、風間智彦

    日本育種学会第137回講演会  2020.10 

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    Event date： 2020.10

    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：オンライン   Country：Japan  

  • ミトコンドリア移行TALENを用いた細胞質雄性不稔性関連遺伝子orf352の破壊と表現型の調査

    大向志穂、鳥山欽哉、有村慎一、風間智彦

    日本育種学会第137回講演会  2020.3 

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    Event date： 2020.3

    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：東京大学   Country：Japan  

  • イネ長粒変異体lin1の単離および原因遺伝子同定

    森田竜平、市田裕之、石井公太郎、林依子、安部弘、白川侑希、一瀬勝紀、常泉和秀、風間智彦、鳥山欽哉、佐藤雅志、阿部知子

    日本育種学会第137回講演会  2020.3 

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    Event date： 2020.3

    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：東京大学   Country：Japan  

  • 細胞質雄性不稔植物を用いたミトコンドリアゲノム編集 Invited

    風間 智彦

    プロジェクト横断型公開シンポジウム「植物のゲノム編集基盤技術開発の現状と展望」  2020.2 

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    Event date： 2020.2

    Language：Japanese  

    Country：Japan  

  • TALENを用いたイネミトコンドリアゲノムの改変

    風間智彦, 鳥山欽哉, 有村慎一

    九州育種談話会  2019.11 

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    Event date： 2019.11 - 2020.11

    Language：Japanese   Presentation type：Symposium, workshop panel (public)  

    Venue：熊本   Country：Japan  

  • ミトコンドリア移行TALENによるRT102型CMSイネミトコンドリアゲノムの構造変化と表現型の調査

    大向詩穂・鳥山 欽哉・有村 慎一・風間 智彦

    第14回東北育種研究集会  2019.11 

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    Event date： 2019.11

    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：岩手大学（盛岡）   Country：Japan  

  • TALENを用いた植物ミトコンドリアゲノムの改変 Invited

    風間 智彦

    植物バイオ研究会 第19回会合  2019.11 

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    Event date： 2019.11

    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：東京   Country：Japan  

  • イネ品種 Lebed に台中 65 号を戻し交雑して得られた雄性不稔系統における原因候補遺伝子の解析

    藤田悠生・風間智彦・鳥山欽哉

    日本育種学会第136回講演会  2019.9 

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    Event date： 2019.9

    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：近畿大学（奈良）   Country：Japan  

  • 核コードRETROGRADE-REGULATED MALE STERILITY 遺伝子のゲノム編集によるCW 型細胞質雄性不稔イネの稔性回復

    助友千尋・風間智彦・鳥山欽哉

    日本育種学会第136回講演会  2019.9 

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    Event date： 2019.9

    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：近畿大学（奈良）   Country：Japan  

  • CW-CMS/Rf17システムを用いたハイブリッドライス開発のための日印交雑におけるF1の雑種強勢

    福田善通・斉藤大樹・冨田朝美・風間智彦・佐藤雅志・岡正明・鳥山欽哉

    日本育種学会第135回講演会  2019.3 

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    Event date： 2019.3

    Language：Japanese  

    Venue：千葉大学（千葉）   Country：Japan  

  • Morphological and expression analysis under low temperature condition in rice anther development International conference

    Kaneko F, Fujioka T, Kazama T, Park J. I, Suwabe K, Endo M, Nagano K, Kawagishi-Kobayashi M, Watanabe M

    Plant & Animal Genomes XVI Conference in San Diego, U.S.A. (2008)  2008.1 

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    Language：Others  

    Country：Other  

    Morphological and expression analysis under low temperature condition in rice anther development

  • Identification and cheracterization of new small RNAs associated with male organ differentiation in rice International conference

    Fujioka T, Kaneko F, Kazama T, Takada Y, Suwabe K, Suzuki G, Endo M, Kawagishi-Kobayashi M, Watanabe M

    Plant & Animal Genomes XVI Conference in San Diego, U.S.A. (2008)  2008.1 

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    Language：Others  

    Country：Other  

    Identification and cheracterization of new small RNAs associated with male organ differentiation in rice

  • イネの花粉形成に関与するPPR遺伝子の解析

    風間智彦, 鳥山欽哉

    第49回日本植物生理学会年会  2008.3 

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    Language：Others  

    Venue：札幌   Country：Other  

    本人が発表

  • Characterization of specific genes in reproductive stage under cold stress in rice International conference

    Kazama T, Kaneko F, Takada Y, Suwabe K, Suzuki G, Endo M, Kawagishi-Kobayashi M, Watanabe M

    FESPB2008  2008.8 

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    Country：Other  

    Characterization of specific genes in reproductive stage under cold stress in rice
    本人が発表

  • イネLD型細胞質雄性不稔性に対する稔性回復遺伝子はグリシンリッチタンパク質をコードする。

    板橋悦子, 岩田夏子, 藤井壮太, 風間智彦, 鳥山欽哉

    第51回日本植物生理学会年会  2010.3 

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    Country：Other  

  • Oryza rufipogon由来の細胞質を持つ稔性回復系統Rt61C, RT98C, RT102Cの比較解析

    中村翼, 本村恵二, 鳥山欽哉

    日本育種学会第117回講演会  2010.3 

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    Venue：京都   Country：Other  

  • Fertility restorer gene Rf2 for Lead rice-type cytoplasmic male sterility of rice encodes mitochondrial glycine-rich protein. International conference

    Itabashi, E, Iwata, N, Fujii, S, Kazama, T, Toriyama, K

    International Symposium of Cell-Cell Communication in Plant reproduction.  2010.3 

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    Country：Other  

    Fertility restorer gene Rf2 for Lead rice-type cytoplasmic male sterility of rice encodes mitochondrial glycine-rich protein.

  • イネミトコンドリア遺伝子のRNAエディティングに関わるPPRタンパク質MPR25

    戸田拓士, 藤井壮太, 風間智彦, 鳥山欽哉

    NIASシンポジウム イネ遺伝学・分子生物学ワークショップ2010  2010.7 

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    Country：Other  

  • イネミトコンドリア遺伝子のnad5の転写後発現制御に関わるMPR25タンパク質

    戸田拓士, 藤井壮太, 風間智彦, 鳥山欽哉

    第５回東北育種研究集会  2010.8 

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    Country：Other  

  • Oryza rufipogonに由来する３種類の稔性回復系統RT61C, RT98C, RT102Cの持つ稔性回復遺伝子の探索。

    村田隼人, 風間智彦, 本村恵二, 鳥山欽哉

    第５回東北育種研究集会  2010.8 

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    Country：Other  

  • LD型細胞質雄性不稔イネに対する稔性回復遺伝子Rf2のマップベースクローニングおよび解析。

    板橋悦子, 岩田夏子, 藤井壮太, 風間智彦, 鳥山欽哉

    第５回東北育種研究集会  2010.8 

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    Country：Other  

  • イネミトコンドリア遺伝子のRNAエディティングに関わるPPRタンパク質。

    戸田拓士, 藤井壮太, 風間智彦, 鳥山欽哉

    第２８回日本植物細胞分子生物学会大会  2010.9 

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  • イネミトコンドリアにコードされる遺伝子転写産物の末端に関する研究。

    風間智彦, 鳥山欽哉

    日本育種学会第118回講演会  2010.9 

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  • イネミトコンドリア遺伝子発現制御に関わるMPR25タンパク質の機能解析。

    戸田拓士, 藤井壮太, 野口航, 風間智彦, 鳥山欽哉

    日本育種学会第118回講演会  2010.9 

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  • イネLD型細胞質雄性不稔性に対する稔性回復遺伝子Rf2の単離および解析。

    板橋悦子, 岩田夏子, 藤井壮太, 風間智彦, 鳥山欽哉

    第２８回日本植物細胞分子生物学会大会  2010.9 

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  • イネLD型細胞質雄性不稔性に対する稔性回復遺伝子Rf2はグリシンリッチタンパク質をコードする。

    板橋悦子, 岩田夏子, 藤井壮太, 風間智彦, 鳥山欽哉

    日本育種学会第118回講演会  2010.9 

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  • Oryza rufipogonに由来する細胞質雄性不稔性イネ３系統のミトコンドリアゲノム構造比較。

    村田隼人, 風間智彦, 本村恵二, 鳥山欽哉

    第２８回日本植物細胞分子生物学会大会  2010.9 

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  • Oryza rufipogonに由来するRT61C, RT98C, RT102Cの持つ雄性不稔細胞質の比較解析。

    村田隼人, 風間智彦, 本村恵二, 鳥山欽哉

    日本育種学会第118回講演会  2010.9 

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  • Molecular comparison of fertility restorer genes, Rf1, Rf2 and Rf17, for cytoplasmic male sterility in rice. International conference

    Toriyama, K, Fujii, S, Itabashi, E, Kazama T

    3rd International Rice Congress.  2010.11 

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    Molecular comparison of fertility restorer genes, Rf1, Rf2 and Rf17, for cytoplasmic male sterility in rice.

  • 光合成に影響を及ぼすイネミトコンドリアPentatricopeptide repeat MPR25の機能解析

    戸田拓士, 藤井壮太, 野口航, 有村慎一, 風間智彦, 鳥山欽哉

    第52回 日本植物生理学会年会  2011.3 

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    Venue：日本国 仙台   Country：Other  

  • Molecular analysis of cytoplasmic male sterility and fertility restoration in rice. International conference

    Kinya Toriyama

    1st Congress of Cereal Biotechnology and Breeding  2011.5 

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    Molecular analysis of cytoplasmic male sterility and fertility restoration in rice.

  • Comparison of fertility restoration mechanisms between BT and LD type cytoplasmic male sterile rice International conference

    Etsuko Itabashi, Kinya Toriyama

    1st Congress of Cereal Biotechnology and Breeding  2011.5 

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    Comparison of fertility restoration mechanisms between BT and LD type cytoplasmic male sterile rice

  • 雄性不稔細胞質を持つ野生イネ８系統のミトコンドリアゲノム構造と稔性回復遺伝子の比較

    加納孝紘, 風間智彦, 鳥山欽哉

    日本育種学会第120回講演会  2011.9 

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    Venue：日本国 福井   Country：Other  

  • 雄性不稔細胞質を持つRT102Cにおける稔性回復遺伝子のマッピング

    村田隼人, 風間智彦, 本村恵二, 鳥山欽哉

    日本育種学会第120回講演会  2011.9 

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    Venue：日本国   Country：Other  

  • 光合成に影響を及ぼすイネミトコンドリアPPR MPR25の機能解析

    戸田拓士, 藤井壮太, 野口航, 有村慎一, 風間智彦, 鳥山欽哉

    日本育種学会第120回講演会  2011.9 

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    Venue：日本国 福井   Country：Other  

  • Molecular analysis of partial fertility restorer gene, Rf2, for BT-type cytoplasmic male sterile rice. International conference

    Kazama, T, Itabashi, E, Toriyama, K

    Plant Reproduction for Food  2012.2 

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    Molecular analysis of partial fertility restorer gene, Rf2, for BT-type cytoplasmic male sterile rice.

  • Fertility restoration in three types of cytoplasmic male sterility in rice. International conference

    Toriyama, K, Kazama, T

    Plant Reproduction for Food  2012.2 

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    Fertility restoration in three types of cytoplasmic male sterility in rice.

  • Regulatory mechanisms of ORF79 translation by RF2 in cytoplasmic male sterile rice

    Kazama, T, Itabashi, E, Fujii, S, Toriyama, K

    2012.3 

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    Regulatory mechanisms of ORF79 translation by RF2 in cytoplasmic male sterile rice

  • 雄性不稔性細胞質を持つ野生イネのミトコンドリアゲノム構造と稔性回復遺伝⼦の解析

    加納孝紘, 風間智彦, 鳥山欽哉

    イネ遺伝学・分子生物学ワークショップ  2012.7 

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  • 細胞質雄性不稔イネRT98Aに対する稔性回復遺伝子のマッピング

    五十嵐圭介, 風間智彦, 本村恵二, 鳥山欽哉

    第30回日本植物細胞分子生物学会  2012.8 

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    Venue：奈良   Country：Other  

  • ナガイモレクチンDB1遺伝子を導入したウンカ抵抗性粗飼料イネの作出

    吉村昌一郎, 小松正明, 角康一郎, 小川智久, 村本光二, 風間智彦, 伊藤幸博, 鳥山欽哉

    第30回日本植物細胞分子生物学会  2012.8 

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    Venue：奈良   Country：Other  

  • イネ開花期における斑点米カメムシの誘引物質合成に関わる遺伝子の解析

    菅谷和音, 堀雅敏, 鳥山欽哉, 風間智彦

    第7回東北育種研究集会  2012.8 

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  • イネLD型細胞質雄性不稔系統における稔性回復因子RF2と相互作用するタンパク質の探索

    藤井慎也, 風間智彦, 伊藤幸博, 小島創一, 鳥山欽哉

    第30回日本植物細胞分子生物学会  2012.8 

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    Venue：奈良   Country：Other  

  • WA型細胞質雄性不稔イネミトコンドリアにおけるorfB遺伝子の転写語制御に関する研究

    平脇佳弥, 風間智彦, 鳥山欽哉

    第30回日本植物細胞分子生物学会  2012.8 

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    Venue：奈良   Country：Other  

  • 雄性不稔細胞質を持つ3種類の野生イネにおけるRf1アリルの比較解析

    加納孝紘, 風間智彦, 鳥山欽哉

    日本育種学会第122回講演会  2012.9 

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  • 植物ミトコンドリア遺伝子発現の分子基盤解明と育種への応用

    中村崇裕

    日本育種学会第122回講演会  2012.9 

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  • コメ産業の国際化を狙った新規ハイブリッドライス育種基盤の開発

    鳥山欽哉

    日本育種学会第122回講演会  2012.9 

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    Venue：京都   Country：Other  

  • イネにおけるミトコンドリア遺伝性の花粉発育不全とそれをレスキューする核遺伝子

    鳥山欽哉

    日本遺伝学会第84回大会  2012.9 

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    Venue：福岡   Country：Other  

  • LD型細胞質雄性不稔イネに対する稔性回復因子RF2と相互作用するタンパク質の解析

    藤井慎也, 風間智彦, 伊藤幸博, 小島創一, 鳥山欽哉

    日本育種学会第122回講演会  2012.9 

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  • CMS系統RT98Aと稔性回復系統RT98CにおけるミトコンドリアmRNAの比較

    五十嵐圭介, 風間智彦, 本村恵二, 鳥山欽哉

    日本育種学会第122回講演会  2012.9 

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  • イネ花粉発達にみるミトコンドリアと核のゲノム相関～ミトコンドリア遺伝子転写後制御に関わるRestorer of fertility-like pentatricopeptide repeat protein genes

    平脇佳弥, 風間智彦, 鳥山欽哉

    新学術領域研究「ゲノム・遺伝子相関」若手の会  2012.10 

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  • Search for proteins that interact with RF2, a restorer of fertility of LD-type cytoplasmic male sterility in rice. 10th International Congress on Plant Molecular Biology International conference

    Fujii, S, Kazama, T, Ito, Y, Kojima, S, Toriyama, K

    10th International Congress on Plant Molecular Biology  2012.10 

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    Search for proteins that interact with RF2, a restorer of fertility of LD-type cytoplasmic male sterility in rice. 10th International Congress on Plant Molecular Biology

  • Identification of cytoplasmic male sterility associated-gene in RT102 cytoplasm derived from Oryza rufipogon. International conference

    Kazama, T, Okazaki, M, Murata, H, Motomura, K, Toriyama, K

    Endosymbiosis from Prokaryotes to Eukaryotic organelles International Meeting  2012.10 

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    Identification of cytoplasmic male sterility associated-gene in RT102 cytoplasm derived from Oryza rufipogon.

  • Comparison of mitochondrial transcripts between RT98A cytoplasmic male sterility line and RT98C fertility restorer line. International conference

    Igarashi, K, Kazama, T, Motomura, K, Toriyama, K

    10th International Congress on Plant Molecular Biology  2012.10 

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    Comparison of mitochondrial transcripts between RT98A cytoplasmic male sterility line and RT98C fertility restorer line.

  • Whole genomic sequencing of cytoplasmic male sterility (CMS) mitochondria derived from Oryza rufipogon to uncover CMS-associated genes. International conference

    Toriyama, K, Igarashi, K, Okazaki, M, Murata, H, Motomura, K, Kazama, T

    10th International Symposium on Rice Functional Genomics  2012.11 

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    Whole genomic sequencing of cytoplasmic male sterility (CMS) mitochondria derived from Oryza rufipogon to uncover CMS-associated genes.

  • Rice MPR25 encodes a pentatricopeptide repeat protein and is not only essential for RNA editing of nad5 transcripts in mitochondria but for chloroplast function. International conference

    Toda, T, Fujii S, Noguchi, K, Kazama, T, Toriyama, K

    10th International Symposium on Rice Functional Genomics  2012.11 

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    Rice MPR25 encodes a pentatricopeptide repeat protein and is not only essential for RNA editing of nad5 transcripts in mitochondria but for chloroplast function.

  • Analysis of mitochondrial genes causing pollen abortion and restorer of fertility genes in Oryza rufipogon. International conference

    Kano, T, Kazama, T, Toriyama, K

    10th International Symposium on Rice Functional Genomics  2012.11 

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    Analysis of mitochondrial genes causing pollen abortion and restorer of fertility genes in Oryza rufipogon.

  • A yeast two hybrid screening to search for protein that interact with RF2, a restorer of fertility of LD-type cytoplasmic male sterility in rice. International conference

    Fujii, S, Kazama, T, Ito, Y, Kojima, S, Toriyama, K

    10th International Symposium on Rice Functional Genomics  2012.11 

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    A yeast two hybrid screening to search for protein that interact with RF2, a restorer of fertility of LD-type cytoplasmic male sterility in rice.

  • 細胞質によって稔性回復効果が異なる稔性回復因子RF2の機能解析

    風間智彦, 鳥山欽哉

    岡山大学共同研究拠点ワークショップ「植物ミトコンドリア研究の新展開」  2013.1 

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  • 野生イネ（Oryza rufipogon）8系統における稔性回復遺伝子と細胞質雄性不稔性原因遺伝子atp6-orf79の比較

    加納孝紘, 風間智彦, 鳥山欽哉

    第54回日本植物生理学会年会  2013.3 

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  • 酵母ツーハイブリッド法とプルダウンアッセイによるイネLD型細胞質雄性不稔系統の稔性回復因子RF2と相互作用する因子の探索

    藤井慎也, 風間智彦, 伊藤幸博, 小島創一, 鳥山欽哉

    第54回日本植物生理学会年会  2013.3 

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  • T98型細胞質雄性不稔性イネにおけるCMS原因遺伝子候補orf113の同定

    五十嵐圭介, 風間智彦, 本村恵二, 鳥山欽哉

    第54回日本植物生理学会年会  2013.3 

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  • Comparison of fertility restoration system in two types of cytoplasmic male sterility in rice. International conference

    Kazama T, Toriyama K

    8th International Conference for Plant Mitochondrial Biology  2013.5 

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    Comparison of fertility restoration system in two types of cytoplasmic male sterility in rice.

  • イネのミトコンドリア遺伝性の雄性生殖器官発育不全とそれをレスキューする核遺伝子

    鳥山欽哉, 風間智彦

    第2回マトリョーシカ型生物学研究会  2013.7 

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  • Struggle between mitochondria and nuclei exemplified by LD-type cytoplasmic male sterility in rice.

    Fujii, S, Kazama, T, Toriyama, K

    2013.7 

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    Struggle between mitochondria and nuclei exemplified by LD-type cytoplasmic male sterility in rice.

  • Struggle between mitochondria and nuclei exemplified by RT98-type cytoplasmic male sterility in rice.

    Igarashi, K, Kazama, T, Toriyama, K

    2013.7 

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    Struggle between mitochondria and nuclei exemplified by RT98-type cytoplasmic male sterility in rice.

  • Struggle between mitochondria and nuclei exemplified by RT102-type cytoplasmic male sterility in rice.

    Okazaki, M, Kazama, T, Toriyama, K

    2013.7 

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    Struggle between mitochondria and nuclei exemplified by RT102-type cytoplasmic male sterility in rice.

  • 細胞質雄性不稔性イネRT02系統に依存するミトコンドリア原因遺伝子の探索

    岡崎真之, 風間智彦, 本村恵二, 鳥山欽哉

    第31回日本植物分子生物学会  2013.9 

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    Venue：札幌   Country：Other  

  • 稔性回復因子RF2と相互作用を示すミトコンドリア局在因子の解析

    藤井慎也, 風間智彦, 伊藤幸博, 小島創一, 鳥山欽哉

    第31回日本植物分子生物学会  2013.9 

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    Venue：札幌   Country：Other  

  • orf79をミトコンドリアに持つ細胞質雄性不稔イネの比較解析

    風間智彦, 藤井慎也, 鳥山欽哉

    第31回日本植物分子生物学会  2013.9 

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    Venue：札幌   Country：Other  

  • RT98型細胞質雄性不稔性原因遺伝子候補orf113の同定とリコンビナントタンパク質の毒性調査

    五十嵐圭介, 風間智彦, 本村恵二, 鳥山欽哉

    第31回日本植物分子生物学会  2013.9 

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    Venue：札幌   Country：Other  

  • 開花期のイネから放出される香気成分の品種間差異

    菅谷和音, 堀雅敏, 鳥山欽哉, 風間智彦

    日本育種学会124回講演会  2013.10 

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    Venue：鹿児島   Country：Other  

  • 細胞質雄性不稔イネにおける稔性回復遺伝子Rf2の稔性回復効果の違いについての解析

    風間智彦, 藤井慎也, 板橋悦子, 本村恵二, 鳥山欽哉

    日本育種学会124回講演会  2013.10 

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    Venue：鹿児島   Country：Other  

  • イネRT98型CMS原因遺伝子orf113の稔性回復系統におけるRNAプロセッシング

    五十嵐圭介, 風間智彦, 本村恵二, 鳥山欽哉

    日本育種学会124回講演会  2013.10 

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    Venue：鹿児島   Country：Other  

  • イネRT102型CMS原因遺伝子orf352の稔性回復系統におけるRNAプロセッシング

    岡崎真之, 風間智彦, 本村恵二, 鳥山欽哉

    日本育種学会124回講演会  2013.10 

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    Venue：鹿児島   Country：Other  

  • イネLD型CMS系統における稔性回復因子RF2と相互作用するubiquitin domain containing proteinの解析

    藤井慎也, 風間智彦, 伊藤幸博, 小島創一, 鳥山欽哉

    日本育種学会124回講演会  2013.10 

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    Venue：鹿児島   Country：Other  

  • Correlative gene system between mitochondria and nuclei observed in RT98-type cytoplasmic male sterility in rice.

    Igarashi, K, Kazama, T, Toriyama, K

    2013.10 

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    Correlative gene system between mitochondria and nuclei observed in RT98-type cytoplasmic male sterility in rice.

  • ミトコンドリアRNAの制御が花粉の運命を決定するミトコンドリアRNAの制御が花粉の運命を決定する

    風間智彦, 鳥山欽哉

    第３回植物RNA研究ネットワークシンポジウム、「RNAから植物を考える – 植物をささえるRNA機能 -」  2013.11 

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  • Ubiquitin domain containing protein interacts with RF2, a restorer of fertility of LD-type cytoplasmic male sterility in rice. International conference

    Fujii, S, Kazama, T, Ito, Y, Kojima, S, Toriyama, K

    7th International Rice Genetics Symposium  2013.11 

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    Ubiquitin domain containing protein interacts with RF2, a restorer of fertility of LD-type cytoplasmic male sterility in rice.

  • Determination of 5' and 3' ends of orf113 RNA associated with RT98-type cytoplasmic male sterility in rice. International conference

    Igarashi, K, Kazama, T, Motomyra, K, Toriyama, K

    7th International Rice Genetics Symposium  2013.11 

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    Determination of 5' and 3' ends of orf113 RNA associated with RT98-type cytoplasmic male sterility in rice.

  • Comparative study on cytoplasmic male sterility-associated genes, orf79, orf113, orf352, derived from Oryza rufipogon. International conference

    Toriyama, K, Igarashi, K, Okazaki, M, Motomura, K, Kazama, T

    7th International Rice Genetics Symposium  2013.11 

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    Comparative study on cytoplasmic male sterility-associated genes, orf79, orf113, orf352, derived from Oryza rufipogon.

  • ハイブリッドライス育種基盤の開発

    鳥山欽哉, 風間智彦

    東北大学イノベーションフェア2014  2014.1 

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  • 植物のメス化：ミトコンドリアが花粉を殺す？

    風間智彦, 鳥山欽哉

    第二回 YoungMito2014  2014.5 

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  • イネRT98型細胞質雄性不稔性に対する稔性回復遺伝子のファインマッピング

    五十嵐圭介, 風間智彦, 本村恵二, 鳥山欽哉

    日本育種学会第126回講演会  2014.9 

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    Venue：都城   Country：Other  

  • イネRT102型細胞質雄性不稔性に対する稔性回復遺伝子のファインマッピング

    岡崎真之, 村田隼人, 風間智彦, 本村恵二, 鳥山欽哉

    日本育種学会第126回講演会  2014.9 

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    Venue：都城   Country：Other  

  • WA型細胞質雄性不稔性イネに対する稔性回復遺伝子Rf4はPPRタンパク質をコードする

    風間智彦, 鳥山欽哉

    日本育種学会第126回講演会  2014.9 

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    Venue：都城   Country：Other  

  • LD型細胞質雄性不稔性イネに対する稔性回復因子RF2複合体の探索

    藤井慎也, 風間智彦, 藤原正幸, 鳥山欽哉

    日本育種学会第126回講演会  2014.9 

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    Venue：都城   Country：Other  

  • イネ科葉緑体内包膜における蛋白質透過装置TICトランスロコンの同定

    小谷佳子, 風間智彦, 菊地真吾, 朝倉由香里, 鳥山欽哉, 中井 正人

    第37回日本分子生物学会年会  2014.11 

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    Country：Other  

    大阪大学との共同研究

  • Mitochondrial genotype determines fate of pollen development in cytoplasmic male sterility International conference

    Keisuke Igarashi, Masayuki Okazaki, Kinya Toriyama

    9th International Conference for Plant Mitochondrial Biology  2015.5 

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    Country：Other  

    Mitochondrial genotype determines fate of pollen development in cytoplasmic male sterility

  • イネRT98型細胞質雄性不稔系統と可稔系統の葯遺伝子発現比較解析

    五十嵐圭介, 風間智彦, 倉田哲也, 鳥山欽哉

    第33回日本植物細胞分子生物学会大会・シンポジウム  2015.8 

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    Country：Other  

  • WA型細胞質雄性不稔イネの稔性回復因子RF4はミトコンドリアにおいてWA352 RNAの分解に関与する

    風間智彦, 鳥山欽哉

    第33回日本植物細胞分子生物学会大会・シンポジウム  2015.8 

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  • 野生イネの第10 染色体に存在するBT型細胞質雄性不稔イネに対する稔性回復遺伝子の解析

    風間智彦, 五十嵐圭介, 岡崎真之, 鳥山欽哉

    日本育種学会第128回講演会  2015.9 

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    Venue：新潟   Country：Other  

  • 世界のイネ・コアコレクションにおける柱頭露出性の評価

    川出駿, 鳥山欽哉, 風間智彦

    日本育種学会第128回講演会  2015.9 

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    Venue：新潟   Country：Other  

  • RNA-seqで細胞質雄性不稔系統RT98Aと維持系統T65で発現が異なる遺伝子の解析

    五十嵐圭介, 風間智彦, 倉田哲也, 鳥山欽哉

    日本育種学会第128回講演会  2015.9 

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    Venue：新潟   Country：Other  

  • CW型細胞質を利用したIR24の細胞質雄性不稔系統の作出

    鳥山欽哉, 風間智彦

    日本育種学会第128回講演会  2015.9 

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    Venue：新潟   Country：Other  

  • 細胞質雄性不稔イネへの変異源処理による稔性回復復帰変異体スクリーニングの試み

    風間智彦, 阿部知子, 鳥山欽哉

    第10回東北育種研究集会  2015.11 

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  • 世界のイネコアコレクションに由来する新規細胞質雄性不稔系統作出の試み

    村上哲也, 風間智彦, 鳥山欽哉

    第10回東北育種研究集会  2015.11 

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  • スリランカ在来品種KALUHEENATIに由来する細胞質雄性不稔性とその稔性回復遺伝子の研究

    志田怜那, 風間智彦, 鳥山欽哉

    第10回東北育種研究集会  2015.11 

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  • イネ開花期における香気成分の合成・放出に関わる遺伝子の解析

    鎌田茉李恵, 菅谷和音, 堀雅敏, 鳥山欽哉, 風間智彦

    第10回東北育種研究集会  2015.11 

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  • 野生イネOryza rufipogon2系統における第10染色体Rf1領域の解析

    イネ属近縁野生種研究会  2016.7 

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    Venue：日本国   Country：Other  

  • Genetic analysis of fertility restorer locus o chromosome 10 derived from a wild rice. International conference

    Keisuke Igarashi, Masayuki Okazaki, Kinya Toriyama

    Frontiers in plant mitochondrial genome research  2016.7 

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    Genetic analysis of fertility restorer locus o chromosome 10 derived from a wild rice.

  • 世界のイネ・コアコレクションに由来する新規細胞質雄性不稔系統の育成

    村上哲也, Dini Astika Sari, 五十嵐圭介, 風間智彦, 鳥山欽哉

    日本育種学会第130回講演会  2016.9 

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    Venue：鳥取   Country：Other  

  • orf79を保有する様々なイネにおけるorf79発現制御の比較解析

    志田怜那, 長恵理子, 風間智彦, 鳥山欽哉

    日本育種学会第130回講演会  2016.9 

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    Venue：鳥取   Country：Other  

  • Boro型細胞質雄性不稔性イネのミトコンドリアゲノムの解読

    風間智彦, 鳥山欽哉

    日本育種学会第130回講演会  2016.9 

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    Venue：鳥取   Country：Other  

  • 細胞質雄性不稔イネの稔性回復メカニズムの分子遺伝学的解析 Invited

    風間 智彦

    日本育種学会第131回講演会  2017.3 

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  • CW型細胞質とRf17遺伝子を利用したIR64とBasmatiの細胞質雄性不稔系統と稔性回復系統の作出

    鳥山欽哉, 風間智彦

    日本育種学会第131回講演会  2017.3 

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  • Comparative analysis of cytoplasmic male sterility developed from various rice Invited International conference

    Kazama T, Igarashi K, Toriyama K

    XIX International Botanical Congress  2017.7 

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    Comparative analysis of cytoplasmic male sterility developed from various rice

  • 野生イネに由来するRf1相同遺伝子の分子遺伝学的解析

    小方郁弥, 鳥山欽哉, 風間智彦

    日本育種学会第132回講演会  2017.10 

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  • イネ品種LEBEDに台中65号を連続戻し交雑することで得られた雄性不稔系統の遺伝学的解析

    村上哲也, 風間智彦, 鳥山欽哉

    2017.10 

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  • イネミトコンドリアに存在するプラスミド様環状DNAの分子遺伝学的解析

    阿部瑞希, 風間智彦, 鳥山欽哉

    日本育種学会第132回講演会  2017.10 

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  • 野生イネW1112に由来する細胞質雄性不稔系統と稔性回復系統の分子遺伝学的解析

    小松千春, 志田怜那, 長恵理子, 鳥山欽哉, 風間智彦

    日本育種学会第133回講演会  2018.3 

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  • イネ培養細胞を用いたミトコンドリア形質転換系開発の試み

    阿部瑞希, 風間智彦, 鳥山欽哉

    日本育種学会第133回講演会  2018.3 

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  • イネ品種LEBEDに台中65号を連続戻し交雑することでえられた雄性不稔系統における原因遺伝子のマッピング

    村上哲也, 風間智彦, 鳥山欽哉

    日本育種学会第133回講演会  2018.3 

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  • CW型細胞質とRf17遺伝子を利用した主要インディカ7品種の細胞質雄性不稔・稔性回復パネル育成

    鳥山欽哉, 風間智彦, 福田善通, 岡正明

    日本育種学会第134回講演会  2018.9 

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  • 細胞質雄性不稔性イネへの重イオンビームとEMS処理による稔性回復変異体の解析

    風間智彦, 市田裕之, 阿部知子, 佐藤雅志, 鳥山欽哉

    第13回 東北育種研究集会  2018.11 

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  • 稔性回復遺伝子Rf1はW1112型細胞質雄性不稔性イネの稔性を回復させるか？

    佐々木実穂, 小松千春, 志田怜那, 長恵理子, 鳥山欽哉, 風間智彦

    第13回 東北育種研究集会  2018.11 

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  • ミトコンドリア移行TALENを用いたCW型細胞質雄性不稔性イネの雄性不稔性原因遺伝子の破壊

    木下諄美, 有村慎一, 鳥山欽哉, 風間智彦

    第13回 東北育種研究集会  2018.11 

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  • ミトコンドリア移行TALENを用いたCMS原因遺伝子候補orf352のノックアウト

    大向詩穂, 有村慎一, 鳥山欽哉, 風間智彦

    第13回 東北育種研究集会  2018.11 

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  • ミトコンドリア移行TALENを用いた植物ミトコンドリアゲノム改変の試み

    池田健一郎, 有村慎一, 鳥山欽哉, 風間智彦

    第13回 東北育種研究集会  2018.11 

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  • Mitochondrial genome modification using mitochondria-targeted TALEN International conference

    WUR-TU Science workshop2018  2018.12 

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    Mitochondrial genome modification using mitochondria-targeted TALEN

  • mitoTALENによるミトコンドリアゲノム編集：BT型細胞質雄性不稔性イネにおけるミトコンドリア遺伝子orf79の破壊

    風間智彦, 亘悠太, 堤伸浩, 鳥山欽哉, 有村慎一

    日本育種学会第135回講演会  2019.3 

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  • mitoTALENによるミトコンドリアゲノム編集：ナタネ細胞質雄性不稔関連遺伝子orf125の遺伝子破壊と稔性回復

    鶴田遊, 菅谷元, 柳瀬俊吾, 亘悠太, 風間智彦, 肥塚千恵, 堤伸浩, 肥塚信也, 有村慎一

    日本育種学会第135回講演会  2019.3 

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  • CW-CMS/Rf17システムを用いたハイブリッドライス開発のための日院交雑におけるF1の雑種強勢

    福田善通, 斎藤大樹, 冨田朝美, 風間智彦, 佐藤雅志, 岡正明, 鳥山欽哉

    日本育種学会第135回講演会  2019.3 

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MISC

  • The mitochondrial and plastid genomes of Oryza sativa L. cv. Taichung 65

    Ichida H., Kazama T., Arimura S.I., Toriyama K.

    Plant Biotechnology   40 ( 1 )   109 - 112   2023   （ ISSN:13424580 ）

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    Publisher：Plant Biotechnology  

    A highly contiguous mitochondrial and plastid genome sequences of a japonica rice cultivar, Taichung 65, were determined by a hybrid approach with long-and short-read sequences. The assembled mitochondrial genome was 465,453 bases in length with an overall GC content of 43.8%. It was predicted to harbor 62 protein-encoding genes, 16 kinds (33 copies) of transfer RNA, and three kinds (six copies) of ribosomal RNA genes. The mitochondrial genome structure in Taichung 65 is largely the same as that of Nipponbare, but the first ∼9.5 kb sequence in Nipponbare (DQ167400) is replaced with a ∼27 kb sequence duplicated from other parts of the mitochondrial genome. Phylogenetic and sequence polymorphism analysis indicated that Taichung 65 is classified as typical japonica. The assembled plastid genome sequence was 134,551 bases in length and completely identical to the previously reported Nipponbare sequence. These near-complete organelle genome sequences will serve as fundamental resources for investigating alloplasmic cytoplasmic male sterile lines and other organelle-controlled phenomena in rice.

    DOI： 10.5511/plantbiotechnology.22.1213a

    Scopus

  • The mitochondrial and plastid genomes of Oryza sativa L. cv. Taichung 65

    Hiroyuki Ichida, Tomohiko Kazama, Shin Ichi Arimura, Kinya Toriyama

    Plant Biotechnology   40 ( 1 )   109 - 112   2023   （ ISSN:1342-4580 eISSN:1347-6114 ）

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    Publishing type：Rapid communication, short report, research note, etc. (scientific journal)  

    A highly contiguous mitochondrial and plastid genome sequences of a japonica rice cultivar, Taichung 65, were determined by a hybrid approach with long-and short-read sequences. The assembled mitochondrial genome was 465,453 bases in length with an overall GC content of 43.8%. It was predicted to harbor 62 protein-encoding genes, 16 kinds (33 copies) of transfer RNA, and three kinds (six copies) of ribosomal RNA genes. The mitochondrial genome structure in Taichung 65 is largely the same as that of Nipponbare, but the first ∼9.5 kb sequence in Nipponbare (DQ167400) is replaced with a ∼27 kb sequence duplicated from other parts of the mitochondrial genome. Phylogenetic and sequence polymorphism analysis indicated that Taichung 65 is classified as typical japonica. The assembled plastid genome sequence was 134,551 bases in length and completely identical to the previously reported Nipponbare sequence. These near-complete organelle genome sequences will serve as fundamental resources for investigating alloplasmic cytoplasmic male sterile lines and other organelle-controlled phenomena in rice.

    DOI： 10.5511/plantbiotechnology.22.1213a

    Scopus

    researchmap

  • A novel mutation induced by Ar-ion-irradiation affect grain length and improve yield in rice Reviewed

    R. Morita, H. Ichida, Y. Hayashi, H. Abe, Y. Shirakawa, K. Ichinose, T. Kazama, K. Toriyama, T. Sato, T. Abe

    RIKEN Accel. Prog. Rep   2019.10

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    Language：English  

  • Rice organelle genomics: Approaches to genetic engineering and breeding

    Kazama, T., Nishimura, A., Arimura, S.-I.

    2018.3

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    Language：English  

    DOI： 10.1007/978-981-10-7461-5_4

  • Mitochondrial-inherited pollen abortion and nuclear genes recovering fertility in rice

    Kinya Toriyama, Tomohiko Kazama

    GENES & GENETIC SYSTEMS   2012.12

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  • ナガイモレクチンDB1遺伝子を導入したウンカ抵抗性粗飼料用イネの作出

    吉村昌一郎, 小松正明, 角康一郎, 小川智久, 村本光二, 風間智彦, 伊藤幸博, 鳥山欽哉

    日本植物細胞分子生物学会大会・シンポジウム講演要旨集   2012.8

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    Language：Japanese  

  • Multiple Mechanisms and Challenges for the Application of Allopolyploidy in Plants

    Kenji Osabe, Takahiro Kawanabe, Taku Sasaki, Ryo Ishikawa, Keiichi Okazaki, Elizabeth S. Dennis, Tomohiko Kazama, Ryo Fujimoto

    INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES   2012.7

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    Language：English  

    DOI： 10.3390/ijms13078696

  • Suppression mechanism of mitochondrial ORF79 accumulation by Rf1 protein in BT-type cytoplasmic male sterile rice (vol 55, pg 619, 2008)

    T. Kazama, T. Nakamura, M. Watanabe, M. Sugita, K. Toriyama

    PLANT JOURNAL   2011.8

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    Language：English  

    DOI： 10.1111/j.1365-313X.2011.04678.x

  • 細胞質雄性不稔植物の稔性回復遺伝子の正体とは？ PPRタンパク質によるミトコンドリア遺伝子の発現制

    鳥山欽哉, 風間智彦

    化学と生物   2005.4

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    Language：Japanese  

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Industrial property rights

Patent   Number of applications: 1   Number of registrations: 0
Utility model   Number of applications: 0   Number of registrations: 0
Design   Number of applications: 0   Number of registrations: 0
Trademark   Number of applications: 0   Number of registrations: 0

Professional Memberships

  • Japanese Society of Breeding

  • 日本植物生理学会

  • 日本植物細胞分子生物学会

  • Japanese Society of Breeding

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  • 日本植物細胞分子生物学会

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  • 日本植物生理学会

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Committee Memberships

  • Steering committee member   Domestic

    2021.3 - 2022.3   

  • 日本育種学会   集会担当   Domestic

    2021.3 - 2022.3   

  • Steering committee member   Domestic

    2020.3 - 2022.2   

  • 運営委員 集会   集会   Domestic

    2020.3 - 2022.2   

  • 日本育種学会   運営委員 集会  

    2020.3 - 2022.2   

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Academic Activities

  • セッション3「オルガネラゲノム編集」オーガナイザー

    日本ゲノム編集学会第6回大会  （ オンライン ） 2022.6

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    Type：Competition, symposium, etc. 

  • 一般社団法人日本育種学会の運営委員（集会）

    2020.4 - 2022.3

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  • Screening of academic papers

    Role(s)： Peer review

    2019

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    Type：Peer review 

    Number of peer-reviewed articles in foreign language journals：12

Other

  • Breeding Science 論文審査

    2016.9

  • Journal of Heredity 論文審査

    2016.6

  • Journal of Experimental Botany 論文審査

    2016.6

  • Breeding Science 論文審査

    2016.3

  • Plant Physiology 論文審査

    2015.3

  • The Plant Cell 論文審査

    2014.11

  • The Plant Journal 論文審査

    2014.10

  • Journal of Proteomics 論文審査

    2014.10

  • Genes and Genetic Systems 論文審査

    2014.10

  • Mitochondrion 論文審査

    2014.8

  • RNA Biology 論文審査

    2014.5

  • Plant Cell Reports 論文審査

    2014.5

  • Plant Cell Reports 論文審査

    2013.1

  • Genes & Genetic Systemsの論文査読

    2010.9

  • Plant Cell Reports 論文審査

    2010.8

  • Journal of Plant Research 論文審査

    2009.9

  • Plant and Cell Physiology 論文審査

    2009.8

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Research Projects

  • Genomic regulation of intracellular symbiotic organelles: from technological innovation to understanding and application of life phenomena

    Grant number：24H02270  2024.4 - 2029.3

    Grants-in-Aid for Scientific Research  Grant-in-Aid for Transformative Research Areas (A)

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    Grant type：Scientific research funding

    CiNii Research

  • mitoTALENを用いた雄性不稔性発生メカニズムの解明

    Grant number：23K21187  2024.4 - 2025.3

    科学研究費助成事業  基盤研究(B)

    風間 智彦

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    Grant type：Scientific research funding

    CiNii Research

  • mitoTALENを用いた雄性不稔性発生メカニズムの解明

    Grant number：21H02169  2021 - 2024

    日本学術振興会  科学研究費助成事業  基盤研究(B)

    風間 智彦

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    Authorship：Principal investigator  Grant type：Scientific research funding

    収量増加の育種において、細胞質雄性不稔性はハイブリッド種子の効率的な採種を支える基盤であり、農業上非常に重要な形質である。しかし、ミトコンドリアへの細胞質雄性不稔性原因遺伝子（CMS原因遺伝子）産物の蓄積が、なぜ雄性不稔性を引き起こすのか？といった細胞質雄性不稔性発生の分子メカニズムは全く明らかになっていない。そこで、ミトコンドリアに存在する遺伝子をノックアウトすることが可能であるミトコンドリア移行シグナル付きTALEN（mitoTALEN）を用いて、CMS原因遺伝子候補の遺伝子破壊株を作成し、CMS原因遺伝子の特定を行うとともに、雄性不稔性を引き起こすメカニズムに関する知見を得る。

    CiNii Research

  • Establishment of the basis for plant mitochondrial genome breeding

    Grant number：20H05680  2020.8 - 2025.3

    Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (S)

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    Grant type：Scientific research funding

    CiNii Research

  • 植物ミトコンドリアゲノム育種の基盤創出

    Grant number：JAB0H05680  2020 - 2024

    日本学術振興会  科学研究費助成事業  基盤研究(S)

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    Authorship：Coinvestigator(s)  Grant type：Scientific research funding

  • 人工稔性回復因子をモデルとした植物ミトコンドリア遺伝子の人為的制御の試み

    2018 - 2020

    日本学術振興会  科学研究費助成事業  基盤研究(B)

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    Authorship：Principal investigator  Grant type：Scientific research funding

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Class subject

  • 分析化学実験（応用生命化学分野）

    2024.10 - 2025.3   Second semester

  • Seminar in a Specified Field Ⅰ

    2024.4 - 2025.3   Full year

  • Master's Thesis Research Ⅰ

    2024.4 - 2025.3   Full year

  • 分子生物学

    2023.4 - 2023.9   First semester

  • 生物機能分子化学II

    2023.4 - 2023.9   First semester

  • Molecular Biology

    2023.4 - 2023.9   First semester

  • 分子生物学

    2022.4 - 2022.9   First semester

  • 生物機能分子化学 I（E科目）

    2022.4 - 2022.9   First semester

  • 生物機能分子化学II

    2021.4 - 2021.9   First semester

  • Special Lecture on Advanced Topics of Agriculture 5（中村 崇裕）

    2020.4 - 2020.9   First semester

  • 分析化学実験（応用生命化学分野）

    2024.10 - 2025.3   Second semester

  • Master's Thesis Research Ⅰ

    2024.4 - 2025.3   Full year

  • Seminar in a Specified Field Ⅰ

    2024.4 - 2025.3   Full year

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Social Activities

  • 九州大学未来創成科学者育成プロジェクトで1名の高校生を受け入れ

    2024.4

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    Audience： Infants,　Schoolchildren,　Junior students,　High school students

    Type：Other

  • 九州大学QURIESプログラム／2023年8月8日-18日の間の5日間で開催されたQURIESプログラムで2名の高校生を受け入れた．

    2023.9

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    Audience： Infants,　Schoolchildren,　Junior students,　High school students

    Type：Seminar, workshop

  • バイオインダストリー協会より講演依頼があったので、講演を行い、話題提供を行った．

    一般財団法人バイオインダストリー協会  2019.11

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    Audience： General,　Scientific,　Company,　Civic organization,　Governmental agency

    Type：Seminar, workshop

  • バイオインダストリー協会より講演依頼があったので、講演を行い、話題提供を行った．

    一般財団法人バイオインダストリー協会  2019.11

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    Type：Seminar, workshop

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  • 福島県立磐城高等学校 研究室訪問

    2015.4

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    磐城高校1年生が、農学部内の研究室見学を行った際、環境適応生物工学分野の案内を行った。

    researchmap

  • 福島県立磐城高等学校 研究室訪問

    2013.4

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    磐城高校1年生が、農学部内の研究室見学を行った際、環境適応生物工学分野の案内を行った。

    researchmap

  • ベルリン・フンボルト大学 公開セミナー

    2012.11

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    フンボルト大学において、セミナーを行った。

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  • ウルム大学 公開セミナー

    2012.10

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    ウルム大学において、セミナーを行った。

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  • 福島県立磐城高等学校 研究室訪問

    2012.4

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    磐城高校1年生が、農学部内の研究室見学を行った際、環境適応生物工学分野の案内を行った。

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  • 科学者の卵養成講座

    2010.12

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    第2期発展コース（実験コース）で「お米一粒で品種判別せよ！」を担当

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  • 第11回 植物オルガネラワークショップ ーオルガネラの動態と機能分子

    2009.3

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    第50回日本植物生理学会年会のサテライトミーティングとして開催されたオルガネラワークショップにおいて、「細胞質雄性不稔イネの解析から見えてきたもの」という内容で、講演した。この講演は、招待講演である。

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  • 九州大学高等研究機構SSP研究室セミナー

    2008.12

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    九州大学高等研究機構の中村崇裕准教授の研究室で「BT型細胞質雄性不稔イネの稔性回復メカニズム」というタイトルでセミナーを行った。

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Media Coverage

  • 植物ミトコンドリアのゲノム編集に成功 Newspaper, magazine

    日本経済新聞  2019.7

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    植物ミトコンドリアのゲノム編集に成功

Activities contributing to policy formation, academic promotion, etc.

  • 2020.4 - 2022.3  

    一般社団法人日本育種学会の運営委員（集会）