Language：English   Publishing type：Research paper (scientific journal)   Publisher：Japanese Society for Plant Biotechnology  

<p>A highly contiguous mitochondrial and plastid genome sequences of a <i>japonica</i> rice cultivar, Taichung 65, were determined by a hybrid approach with long- and short-read sequences. The assembled mitochondrial genome was 465,453 bases in length with an overall GC content of 43.8%. It was predicted to harbor 62 protein-encoding genes, 16 kinds (33 copies) of transfer RNA, and three kinds (six copies) of ribosomal RNA genes. The mitochondrial genome structure in Taichung 65 is largely the same as that of Nipponbare, but the first ∼9.5 kb sequence in Nipponbare (DQ167400) is replaced with a ∼27 kb sequence duplicated from other parts of the mitochondrial genome. Phylogenetic and sequence polymorphism analysis indicated that Taichung 65 is classified as typical <i>japonica</i>. The assembled plastid genome sequence was 134,551 bases in length and completely identical to the previously reported Nipponbare sequence. These near-complete organelle genome sequences will serve as fundamental resources for investigating alloplasmic cytoplasmic male sterile lines and other organelle-controlled phenomena in rice.</p>

DOI： 10.5511/plantbiotechnology.22.1213a

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Language：English   Publishing type：Research paper (scientific journal)  

DOI： doi.org/10.1007/978-1-0716-2922-2_25

Language：Japanese   Publishing type：Research paper (scientific journal)   Publisher：WILEY  

Cytoplasmic male sterility (CMS) is a trait that causes pollen or anther dysfunctions, resulting in the lack of seed setting. CMS is considered to be caused by the expression of a unique mitochondrial open reading frame referred to as CMS-associated gene. orf312 has been reported as a CMS-associated gene of Tadukan-type CMS (TAA) in rice (Oryza sativa L.), which exhibits impaired anther dehiscence; however, evidence thereof has not yet been reported. Here, we took a loss-of-function approach, using a mitochondria-targeted transcription activator-like effector nuclease (mitoTALEN) designed to knock out orf312 in TAA, to prove that orf312 indeed is a CMS-causative gene. Out of 28 transgenic TAA plants harboring the mitoTALEN expression vector, deletion of orf312 was detected in 24 plants by PCR, Southern blot, and sequencing analyses. The 24 plants were grouped into three groups based on the deleted regions. All orf312-depleted TAA plants exhibited recovery of anther dehiscence and seed setting. The depletion of orf312 and fertility restoration was maintained in the next generation, even in mitoTALEN expression cassette null segregants. In contrast, orf312-retaining plants were sterile. These results provide robust evidence that orf312 is a Tadukan-type CMS-causative gene.

DOI： 10.1111/tpj.15715

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Language：Japanese   Publishing type：Research paper (scientific journal)  

DOI： doi.org/10.1186/s12284-021-00488-7

Language：English   Publishing type：Research paper (scientific journal)  

Language：Japanese   Publishing type：Research paper (scientific journal)  

Language：Others  

LONG GRAIN 1: a novel gene that regulates grain length in rice

DOI： 10.1007/s11032-019-1032-1

Language：Others  

Development of cytoplasmic male sterile lines and restorer lines of various elite Indica Group rice cultivars using CW-CMS/Rf17 system.

DOI： 10.1186/s12284-019-0332-8

Language：English   Publishing type：Research paper (scientific journal)  

Curing cytoplasmic male sterility via TALEN-mediated mitochondrial genome editing.
Sequence-specific nucleases are commonly used to modify the nuclear genome of plants. However, targeted modification of the mitochondria! genome of land plants has not yet been achieved. In plants, a type of male sterility called cytoplasmic male sterility (CMS) has been attributed to certain mitochondria! genes, but none of these genes has been validated by direct mitochondria! gene-targeted modification. Here, we knocked out CMS-associated genes (orf79 and orf125) of CMS varieties of rice and rapeseed, respectively, using transcription activator-like effector nucleases (TALENs) with mitochondria localization signals (mitoTALENs). We demonstrate that knocking out these genes cures male sterility, strongly suggesting that these genes are causes of CMS. Sequencing revealed that double-strand breaks induced by mitoTALENs were repaired by homologous recombination, and that during this process, the target genes and surrounding sequences were deleted. Our results show that mitoTALENs can be used to stably and heritably modify the mitochondria! genome in plants.

DOI： 10.1038/s41477-019-0459-z

Language：English   Publishing type：Research paper (scientific journal)  

Background: Male sterility is a useful agronomic trait for breeding of self-pollinating crops and is often observed in the progenies of hybrids of distantly related species, for example, Oryza sativa L. subsp. indica and O. sativa L. subsp. japonica. To explore new male sterile lines in rice, we performed successive backcrosses using a japonica cultivar Taichung 65 (T65) as a recurrent pollen parent and various indica cultivars as the initial female parents. Findings: We observed male sterile plants in the backcross progeny from an indica cultivar, Lebed. Both fertile and sterile plants were present in the BC4F1 generation. The sterile plants segregated for fertile and sterile plants when backcrossed with T65 in BC5F1, BC6F1 and BC7F1 with a ratio of 1:1. Conversely, all the backcross progenies from the fertile BC4F1 were consistently fertile. Anthers of the male sterile line were stunted and did not shed pollen
cross-sectional observations revealed defects in sporophytic cells. The male sterility appears to be caused by heterozygous alleles derived from T65 and Lebed. A male sterility gene was mapped between two INDEL markers on the long arm of chromosome 10, which corresponded to a 407 kb region in the Nipponbare genome. Conclusions: Since the heterozygous Lebed allele acts as dominant sporophytic pollen killer, it would be useful for recurrent selection breeding of japonica rice.

DOI： 10.1186/s12284-018-0222-5

Language：English   Publishing type：Research paper (scientific journal)  

Cytoplasmic male sterility (CMS) lines in rice, which have the cytoplasm of a wild species and the nuclear genome of cultivated rice, are of value for the study of genetic interactions between the mitochondrial and nuclear genomes. The RT98-type CMS line RT98A and the fertility restorer line RT98C carry the cytoplasm of the wild species Oryza rufipogon and the nuclear genome of the Taichung 65 cultivar (Oryza sativa L.). Based on a classical crossing experiment, fertility is reported to be restored gametophytically by the presence of a tentative single gene, designated Rf98, which is derived from the cytoplasm donor. Fine mapping of Rf98 revealed that at least two genes, which are closely positioned, are required for complete fertility restoration in RT98A. Here, we identified seven pentatricopeptide repeat (PPR) genes that are located within a 170 kb region as candidates for Rf98. Complementation tests revealed that the introduction of one of these PPR genes, PPR762, resulted in the partial recovery of fertility with a seed setting rate up to 9.3&#37;. We conclude that PPR762 is an essential fertility restorer gene for RT98-type CMS. The low rate of seed setting suggested that some other genes near the Rf98 locus are also necessary for the full recovery of seed setting.

DOI： 10.1093/pcp/pcw135

Language：English   Publishing type：Research paper (scientific journal)  

Nuclear genome substitutions between subspecies can lead to cytoplasmic male sterility (CMS) through incompatibility between nuclear and mitochondrial genomes. Boro-Taichung (BT)-type CMS rice was obtained by substituting the nuclear genome of Oryza sativa subsp. indica cultivar Chinsurah Boro II with that of Oryza sativa subsp. japonica cultivar Taichung 65. In BT-type CMS rice, the mitochondrial gene orf79 is associated with male sterility. A complete sequence of the Boro-type mitochondrial genome responsible for BT-type CMS has not been determined to date. Here, we used pyrosequencing to construct the Boro-type mitochondrial genome. The contiguous sequences were assembled into five circular DNA molecules, four of which could be connected into a single circle. The two resulting subgenomic circles were unable to form a reliable master circle, as recombination between them was scarcely detected. We also found an unequal abundance of DNA molecules for the two loci of atp6. These results indicate the presence of multi-partite DNA molecules in the Borotype mitochondrial genome. Expression patterns were investigated for Boro-type mitochondria- specific orfs, which were not found in the mitochondria from the standard japonica cultivar Nipponbare. Restorer of fertility 1 (RF1)-dependent RNA processing has been observed in orf79-containing RNA but was not detected in other Boro-type mitochondria-specific orfs, supporting the conclusion that orf79 is a unique CMS-associated gene in Boro-type mitochondria.

DOI： 10.1371/journal.pone.0159379

Language：English   Publishing type：Research paper (scientific journal)  

Development of cytoplasmic male sterile IR24 and IR64 using CW-CMS/Rf17 system

DOI： 10.1186/s12284-016-0097-2

Language：English   Publishing type：Research paper (scientific journal)  

Cytoplasmic male sterility (CMS) is an important agricultural trait characterized by lack of functional pollen, and caused by ectopic and defective mitochondrial gene expression. The pollen function in CMS plants is restored by the presence of nuclear-encoded restorer of fertility (Rf) genes. Previously, we cloned Rf2, which restores the fertility of Lead Rice (LD)-type CMS rice. However, neither the function of Rf2 nor the identity of the mitochondrial gene causing CMS has been determined in LD-CMS rice. Here, we show that the mitochondrial gene orf79 acts as a CMS-associated gene in LD-CMS rice, similar to its role in BT-CMS rice originating from Chinsurah Boro II, and Rf2 weakly restores fertility in BT-CMS rice. We also show that RF2 promotes degradation of atp6-orf79 RNA in a different manner from that of RF1, which is the Rf gene product in BT-CMS rice. The amount of ORF79 protein in LD-CMS rice was one-twentieth of the amount in BT-CMS rice. The difference in ORF79 protein levels probably accounts for the mild and severe pollen defects in LD-CMS and BT-CMS rice, respectively. In the presence of Rf2, accumulation of ORF79 was reduced to almost zero and 25&#37; in LD-CMS and BT-CMS rice, respectively, which probably accounts for the complete and weak fertility restoration abilities of Rf2 in LD-CMS and BT-CMS rice, respectively. These observations indicate that the amount of ORF79 influences the pollen fertility in two strains of rice in which CMS is induced by orf79.
Significance Statement In plants, incompatibility between nuclear and mitochondrial genotypes causes cytoplasmic male sterility (CMS). Here, we show that RF2, a nuclear-encoded restorer of fertility gene, degrades transcripts of a CMS causative gene, orf79, and that differences in the amount of ORF79 influence pollen fertility in different strains of orf79-induced CMS rice.

DOI： 10.1111/tpj.13135

Language：English   Publishing type：Research paper (scientific journal)  

RNA editing is an essential mechanism that modifies target cytidines to uridine in both mitochondrial and plastid mRNA. Target sites are recognized by pentatricopeptide repeat (PPR) proteins. Using bioinformatics predictions based on the code describing sequence recognition by PPR proteins, we have identified an Arabidopsis editing factor required for editing of atpF in plastids. A loss-of-function mutation in ATPF EDITING FACTOR1 (AEF1, AT3G22150) results in severe variegation, presumably due to decreased plastid ATP synthase levels. Loss of editing at the atpF site is coupled with a large decrease in splicing of the atpF transcript, even though the editing site is within an exon and 53 nucleotides distant from the splice site. The rice orthologue of AEF1, MPR25, has been reported to be required for editing of a site in mitochondrial nad5 transcripts, and we confirm that editing of the same site is affected in the Arabidopsis aef1 mutant. We also show that splicing of chloroplast atpF transcripts is affected in the rice mpr25 mutant. AEF1 is thus highly unusual for an RNA editing specificity factor in that it has functions in both organelles.
Significance Statement The RNA editing factor AEF1/MPR25 is a sequence-specific RNA binding protein with an unusual multi-functional role in editing and splicing of the chloroplast atpF transcript. It is unique (so far) amongst editing specificity factors in also acting in mitochondria, where it recognizes a similar sequence to induce editing of the nad5 transcript.

DOI： 10.1111/tpj.12756

Language：English   Publishing type：Research paper (scientific journal)  

Background: The pollen function of cytoplasmic male sterile (CMS) plants is often recovered by the Restorer of fertility (Rf) gene encoded by the nuclear genome. An Rf gene of Lead rice type CMS, Rf2, encodes a small mitochondrial glycine-rich protein. RF2 is expected to function by interacting with other proteins, because RF2 has no motifs except for glycine-rich domain.
Findings: To elucidate the protein that interacts with RF2, we performed yeast two-hybrid screening. We identified four genes and named RF2-interacting candidate factors (RIF1 to RIF4). A study of subcellular localization demonstrated that only RIF2 was targeted to mitochondria. A pull-down assay using E. coli-produced recombinant GST-tagged RF2 and His-tagged RIF2 confirmed that RF2 interacted with RIF2. RIF2 encodes ubiquitin domain-containing protein.
Conclusions: These results suggest that RIF2 is a candidate factor of a fertility restoration complex of RF2.

DOI： 10.1186/s12284-014-0021-6

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.3389/fpls.2013.00517

Language：English   Publishing type：Research paper (scientific journal)  

Cytoplasmic male sterility (CMS) is a maternally inherited trait in which plants fail to produce functional pollen and is associated with the expression of a novel open reading frame (orf) gene encoded by the mitochondrial genome. An RT102A CMS line and an RT102C fertility restorer line were obtained by successive backcrossing between Oryza rufipogon W1125 and O. sativa Taichung65. Using next-generation pyrosequen-cing, we determined whole-genome sequences of the mitochondria in RT102-CMS cytoplasm. To identify candidates for the CMS-associated gene in RT102 mitochondria, we screened the mitochondrial genome for the presence of specific orf genes that were chimeric or whose products carried predicted transmembrane domains. One of these orf genes, orf352, which showed different transcript sizes depending on whether the restorer of fertility (Rf) gene was present or not, was identified. The orf352 gene was co-transcribed with the ribosomal protein gene rpl5, and the 2.8 kb rpl5-orf352 transcripts were processed into 2.6 kb transcripts with a cleavage at the inside of the orf352 coding region in the presence of the Rf gene. The orf352 gene is an excellent candidate for the CMS-associated gene for RT102-CMS. © The Author 2013.

DOI： 10.1093/pcp/pct102

Language：English   Publishing type：Research paper (scientific journal)  

Cytoplasmic male sterility (CMS) is a maternally inherited trait resulting in the failure to produce functional pollen and is often observed when an alien cytoplasm is transferred into a cultivated species. An RT98A CMS line and an RT98C fertility restorer line were obtained by successive backcrossing between Oryza rufipogon W1109 and Oryza sativa cultivar Taichung 65. To uncover the CMS-associated mitochondrial genes, we determined the complete sequence of the RT98-CMS mitochondrial genome using next-generation pyrosequencing, and searched new open reading frames (orfs) absent in a reported mitochondrial genome of O. sativa Nipponbare. Then, six candidates were selected for the CMS-associated genes based on the criteria in which they were chimeric in structure or encoded a peptide with transmembrane domains. One of the candidates, orf113, showed different transcript sizes between RT98A and RT98C on Northern blot analysis. The orf113 gene was shown to be co-transcribed with atp4 and cox3 encoding ATP synthase F0 subunit 4 and Cyt c oxidase subunit 3, respectively, and their transcripts were distinctly processed in the presence of a fertility restorer gene. Our results indicate that orf113 is a CMS-associated gene of RT98-CMS. © 2012 The Author 2012. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved.

DOI： 10.1093/pcp/pcs177

Language：English   Publishing type：Research paper (scientific journal)  

An allopolyploid is an individual having two or more complete sets of chromosomes derived from different species. Generation of allopolyploids might be rare because of the need to overcome limitations such as co-existing populations of parental lines, overcoming hybrid incompatibility, gametic non-reduction, and the requirement for chromosome doubling. However, allopolyploids are widely observed among plant species, so allopolyploids have succeeded in overcoming these limitations and may have a selective advantage. As techniques for making allopolyploids are developed, we can compare transcription, genome organization, and epigenetic modifications between synthesized allopolyploids and their direct parental lines or between several generations of allopolyploids. It has been suggested that divergence of transcription caused either genetically or epigenetically, which can contribute to plant phenotype, is important for the adaptation of allopolyploids.

DOI： 10.3390/ijms13078696

Language：English   Publishing type：Research paper (scientific journal)  

Rice MPR25 encodes a pentatricopeptide repeat protein and is essential for RNA editing of nad5 transcripts in mitochondria

DOI： 10.1111/j.1365-313X.2012.05091.x.

Language：English   Publishing type：Research paper (scientific journal)  

The expressions of chloroplast and mitochondria genes are tightly controlled by numerous nuclear-encoded proteins, mainly at the post-transcriptional level. Recent analyses have identified a large, plant-specific family of pentatricopeptide repeat (PPR) motif-containing proteins that are exclusively involved in RNA metabolism of organelle genes via sequence-specific RNA binding. A tandem array of PPR motifs within the protein is believed to facilitate the RNA interaction, although little is known of the mechanism. Here, we describe the RNA interacting framework of a PPR protein, Arabidopsis HCF152. First, we demonstrated that a Pfam model could be relevant to the PPR motif function. A series of proteins with two PPR motifs showed significant differences in their RNA binding affinities, indicating functional differences among PPR motifs. Mutagenesis and informatics analysis putatively identified five amino acids organizing its RNA binding surface [the 1st, 4th, 8th, 12th and 'ii'(-2nd) amino acids] and their complex connections. SELEX (Systematic evolution of ligands by exponential enrichment) and nucleobase preference assays determined the nucleobases with high affinity for HCF152 and suggested several characteristic amino acids that may be involved in determining specificity and/or affinity of the PPR/RNA interaction.

DOI： 10.1093/nar/gkr1084

Language：English   Publishing type：Research paper (scientific journal)  

P>Cytoplasmic male sterility (CMS) is associated with a mitochondrial mutation that causes an inability to produce fertile pollen. The fertility of CMS plants is restored in the presence of a nuclear-encoded fertility restorer (Rf) gene. In Lead Rice-type CMS, discovered in the indica variety 'Lead Rice', fertility of the CMS plant is restored by the single nuclear-encoded gene Rf2 in a gametophytic manner. We performed map-based cloning of Rf2, and proved that it encodes a protein consisting of 152 amino acids with a glycine-rich domain. Expression of Rf2 mRNA was detected in developing and mature anthers. An RF2-GFP fusion was shown to be targeted to mitochondria. Replacement of isoleucine by threonine at amino acid 78 of the RF2 protein was considered to be the cause of functional loss in the rf2 allele. As Rf2 does not encode a pentatricopeptide repeat protein, unlike a majority of previously identified Rf genes, the data from this study provide new insights into the mechanism for restoring fertility in CMS.

DOI： 10.1111/j.1365-313X.2010.04427.x

Language：English   Publishing type：Research paper (scientific journal)  

Background: Plant mitochondrial genomes are known for their complexity, and there is abundant evidence demonstrating that this organelle is important for plant sexual reproduction. Cytoplasmic male sterility (CMS) is a phenomenon caused by incompatibility between the nucleus and mitochondria that has been discovered in various plant species. As the exact sequence of steps leading to CMS has not yet been revealed, efforts should be made to elucidate the factors underlying the mechanism of this important trait for crop breeding.
Results: Two CMS mitochondrial genomes, LD-CMS, derived from Oryza sativa L. ssp. indica (434,735 bp), and CW-CMS, derived from Oryza rufipogon Griff. (559,045 bp), were newly sequenced in this study. Compared to the previously sequenced Nipponbare (Oryza sativa L. ssp. japonica) mitochondrial genome, the presence of 54 out of 56 protein-encoding genes (including pseudo-genes), 22 tRNA genes (including pseudo-tRNAs), and three rRNA genes was conserved. Two other genes were not present in the CW-CMS mitochondrial genome, and one of them was present as part of the newly identified chimeric ORF, CW-orf307. At least 12 genomic recombination events were predicted between the LD-CMS mitochondrial genome and Nipponbare, and 15 between the CW-CMS genome and Nipponbare, and novel genetic structures were formed by these genomic rearrangements in the two CMS lines. At least one of the genomic rearrangements was completely unique to each CMS line and not present in 69 rice cultivars or 9 accessions of O. rufipogon.
Conclusion: Our results demonstrate novel mitochondrial genomic rearrangements that are unique in CMS cytoplasm, and one of the genes that is unique in the CW mitochondrial genome, CW-orf307, appeared to be the candidate most likely responsible for the CW-CMS event. Genomic rearrangements were dynamic in the CMS lines in comparison with those of rice cultivars, suggesting that 'death' and possible 'birth' processes of the CMS genes occurred during the breeding history of rice.

DOI： 10.1186/1471-2164-11-209

Language：English   Publishing type：Research paper (other academic)  

Moleculae analysis of CW-type cytoplasmic male sterility and Rf17-mediated fertility restoration for hybeid rice breeding.
Proceeding of The 14th Australian Plant Breeding Conference and 11th Society for the Advancement of Breeding Research in Asia and Oceania (SABRAO) Congress 2009

Language：English   Publishing type：Research paper (scientific journal)  

Rice with LD-type cytoplasmic male sterility (CMS) possesses the cytoplasm of 'Lead Rice' and its fertility is recovered by a nuclear fertility restorer gene Rf1. Rf1 promotes processing of a CMS-associated mitochondrial RNA of atp6-orf79, which consists of atp6 and orf79, in BT-CMS with the cytoplasm of 'Chinsurah Boro II'. In this study, we found that LD-cytoplasm contained a sequence variant of orf79 downstream of atp6. Northern blot analysis showed that atp6-orf79 RNA of LD-cytoplasm was co-transcribed and was processed in the presence of Rf1 in the same manner as in BT-cytoplasm. Western blot analysis showed that the ORF79 peptide did not accumulate in an LD-CMS line, while ORF79 accumulated in a BT-CMS line and was diminished by Rf1. These results suggest that accumulation of ORF79 is not the cause of CMS in LD-cytoplasm and the mechanism of male-sterility induction/fertility restoration in LD-CMS is different from that in BT-CMS.

DOI： 10.1007/s00299-008-0625-7

Language：English   Publishing type：Research paper (scientific journal)  

The male gametophyte and tapetum play different roles during anther development although they are differentiated from the same cell lineage, the L2 layer. Until now, it has not been possible to delineate their transcriptomes due to technical difficulties in separating the two cell types. In the present study, we characterized the separated transcriptomes of the rice microspore/pollen and tapetum using laser microdissection (LM)-mediated microarray. Spatiotemporal expression patterns of 28,141 anther-expressed genes were classified into 20 clusters, which contained 3,468 (12.3%) anther-enriched genes. In some clusters, synchronous gene expression in the microspore and tapetum at the same developmental stage was observed as a novel characteristic of the anther transcriptome. Noteworthy expression patterns are discussed in connection with gene ontology (GO) categories and gene annotations, which are related to important biological events in anther development, such as pollen maturation, pollen germination, pollen tube elongation and pollen wall formation.

DOI： 10.1093/pcp/pcn128

Language：English   Publishing type：Research paper (scientific journal)  

In flowering plants, the male gametophyte, the pollen, develops in the anther. Complex patterns of gene expression in both the gametophytic and sporophytic tissues of the anther regulate this process. The gene expression profiles of the microspore/pollen and the sporophytic tapetum are of particular interest. In this study, a microarray technique combined with laser microdissection (44K LM-microarray) was developed and used to characterize separately the transcriptomes of the microspore/pollen and tapetum in rice. Expression profiles of 11 known tapetum specific-genes were consistent with previous reports. Based on their spatial and temporal expression patterns, 140 genes which had been previously defined as anther specific were further classified as male gametophyte specific (71 genes, 51%), tapetum-specific (seven genes, 5%) or expressed in both male gametophyte and tapetum (62 genes, 44%). These results indicate that the 44K LM-microarray is a reliable tool to analyze the gene expression profiles of two important cell types in the anther, the microspore/pollen and tapetum.

DOI： 10.1093/pcp/pcn124

Language：English   Publishing type：Research paper (scientific journal)  

In BT-type cytoplasmic male sterile rice (Oryza sativa L.) with Chinsurah Boro II cytoplasm, cytoplasmic male sterility (CMS) is caused by an accumulation of the cytotoxic peptide ORF79. The ORF79 protein is expressed from a dicistronic gene atp6-orf79, which exists in addition to the normal atp6 gene in the BT-type mitochondrial genome. The CMS is restored by a PPR (pentatricopeptide-repeat) gene, Rf1, via RNA processing. However, it has not yet been elucidated how the accumulation of ORF79 is reduced by the action of the Rf1 protein. Here, we report that the level of processed orf79 transcripts in the restorer line was reduced to 50% of the unprocessed atp6-orf79 transcripts in the CMS line. Ninety percent of the processed orf79 transcripts, which remained after degradation, were not associated with the ribosome for translation. Our data suggests that the processing of atp6-orf79 transcripts diminishes the expression of orf79 by the translational reduction and degradation of the processed orf79 transcripts.

DOI： 10.1111/j.1365-313X.2008.03529.x

Language：English   Publishing type：Research paper (scientific journal)  

RRM (RNA-recognition motif) domain is important for the post-transcriptional regulation of gene expression including RNA processing. In our previous study, we found one anther- and/or pollen-specific gene (LjRRM1, previously named as LjMfb-U93) in model legume, Lotus japonicus. Because of the richness of genomic information of another model plant, Arabidopsis thaliana, for functional analysis, we identified and characterized the orthologous genes in A. thaliana. By comparison of the partial nucleotide sequence of LjRRM1 to the public database, we identified three homologous genes (AtRBP45a, AtRBP45b, and AtRBP45c) in A. thaliana genome. Based on promoter analysis, both AtRBP45a and AtRBP45c were specifically expressed in immature anther tissues (tapetum cells) and mature pollen grains of transgenic plants. This expression pattern of AtRBP45a and AtRBP45c is quite similar to that of LjRRM1, indicating that AtRBP45a and AtRBP45c would be orthologous to LjRRM1. Because in another previous experiment, it was shown that proteins having RRM domains were related to pre-mRNA maturation, and as a conclusion, it is possible that LjRRM1, AtRBP45a, and AtRBP45c genes encoding RNA-binding proteins are functionally involved in the repression of translation in mature pollen grains in L. japonicus and A. thaliana.

DOI： 10.1266/ggs.81.355

Language：English   Publishing type：Research paper (scientific journal)  

A fertility restorer gene (Rf-1) of [ms-bo] cytoplasmic male sterility (CMS) in rice has been reported to be responsible for the processing of RNA of aberrant atp6 of mitochondria. We have carried out map-based cloning of the Rf-1 gene and found that a 4.7-kb genomic fragment of a restorer line promoted the processing of aberrant atp6 RNA when introduced into a CMS line. The genomic fragment contained a single open reading frame encoding 18 repeats of the 35 amino acid pentatricopeptide repeat (PPR) motif. The cloned PPR gene is a possible candidate of Rf-1. A non-restoring genotype was identified to have deletions within the coding region. (C) 2003 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies.

DOI： 10.1016/S0014-5793(03)00480-0

Event date： 2023.12

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：九州大学   Country：Japan  

Event date： 2023.11

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：つくば国際会議場   Country：Japan  

Event date： 2023.10

Language：English   Presentation type：Oral presentation (general)  

Venue：Philippine International Convention Center, Manila, Philippines   Country：Philippines  

Event date： 2023.9

Language：Japanese  

Venue：神戸大学   Country：Japan  

Event date： 2023.9

Language：Japanese   Presentation type：Symposium, workshop panel (public)  

Venue：神戸大学   Country：Japan  

Event date： 2023.9

Language：Japanese  

Venue：千葉大学   Country：Japan  

Event date： 2023.9

Language：English   Presentation type：Oral presentation (general)  

Venue：Max Planck Institute of Molecular Plant Physiology, Potsdam-Golm   Country：Germany  

Event date： 2023.9

Language：English   Presentation type：Oral presentation (general)  

Venue：HU Berlin   Country：Germany  

Event date： 2023.9

Language：English   Presentation type：Oral presentation (general)  

Venue：INRAE Versailles   Country：France  

Event date： 2023.9

Language：English   Presentation type：Oral presentation (general)  

Venue：INRAE Versailles   Country：France  

Event date： 2023.8

Language：English   Presentation type：Oral presentation (general)  

Venue：IBMP Strasbourg   Country：France  

Event date： 2023.8

Language：English   Presentation type：Oral presentation (general)  

Venue：IBMP Strasbourg   Country：France  

Event date： 2023.8

Language：English  

Venue：Daejeon, Korea   Country：Korea, Republic of  

Event date： 2023.7

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：九州大学   Country：Japan  

Event date： 2023.3

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：東北大学　川内キャンパス   Country：Japan  

Event date： 2022.12

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：山口大学   Country：Japan  

Event date： 2022.11 - 2022.12

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：幕張メッセ   Country：Japan  

Event date： 2022.11

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：東北大学　新青葉山キャンパス   Country：Japan  

Event date： 2022.11

Language：English   Presentation type：Oral presentation (general)  

Venue：Le Meridien Phuket Beach Resort, Phuket, Thailand   Country：Thailand  

Event date： 2022.9

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：帯広畜産大学   Country：Japan  

Event date： 2022.9

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：大阪公立大学 中百舌鳥キャンパス   Country：Japan  

Event date： 2022.5

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：鶴岡市先端研究産業支援センター   Country：Japan  

Event date： 2022.3

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：オンライン   Country：Japan  

Event date： 2021.12

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：オンライン   Country：Japan  

Event date： 2021.9

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：オンライン   Country：Japan  

Event date： 2021.9

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：オンライン   Country：Japan  

Event date： 2021.9

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：オンライン   Country：Japan  

Event date： 2021.9

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：オンライン   Country：Japan  

Event date： 2021.9

Language：Japanese   Presentation type：Oral presentation (invited, special)  

Venue：オンライン   Country：Japan  

Event date： 2021.9

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：オンライン   Country：Japan  

Event date： 2021.6

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：オンライン   Country：Japan  

Event date： 2021.3

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：オンライン   Country：Japan  

Event date： 2021.3

Language：English   Presentation type：Oral presentation (general)  

Venue：Online   Country：Japan  

Event date： 2020.12

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：福島大学（オンライン）   Country：Japan  

Event date： 2020.12

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：福島大学（オンライン）   Country：Japan  

Event date： 2020.12

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：福島大学（オンライン）   Country：Japan  

Event date： 2020.12

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：オンライン開催   Country：Japan  

Event date： 2020.10

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：オンライン   Country：Japan  

Event date： 2020.10

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：オンライン   Country：Japan  

Event date： 2020.3

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：東京大学   Country：Japan  

Event date： 2020.3

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：東京大学   Country：Japan  

Event date： 2020.2

Language：Japanese  

Country：Japan  

Event date： 2019.11 - 2020.11

Language：Japanese   Presentation type：Symposium, workshop panel (public)  

Venue：熊本   Country：Japan  

Event date： 2019.11

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：岩手大学（盛岡）   Country：Japan  

Event date： 2019.11

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：東京   Country：Japan  

Event date： 2019.9

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：近畿大学（奈良）   Country：Japan  

Event date： 2019.9

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：近畿大学（奈良）   Country：Japan  

Event date： 2019.3

Language：Japanese  

Venue：千葉大学（千葉）   Country：Japan  

Language：Others  

Venue：アメリカ合衆国 サンディエゴ   Country：Other  

Morphological and expression analysis under low temperature condition in rice anther development

Language：Others  

Venue：アメリカ合衆国 サンディエゴ   Country：Other  

Identification and cheracterization of new small RNAs associated with male organ differentiation in rice

Language：Others  

Venue：札幌   Country：Other  

イネの花粉形成に関与するPPR遺伝子の解析
本人が発表

Language：Others  

Venue：フィンランド タンペレ   Country：Other  

Characterization of specific genes in reproductive stage under cold stress in rice
本人が発表

Language：Others  

Country：Other  

イネLD型細胞質雄性不稔性に対する稔性回復遺伝子はグリシンリッチタンパク質をコードする。

Language：Others  

Venue：京都   Country：Other  

Oryza rufipogon由来の細胞質を持つ稔性回復系統Rt61C, RT98C, RT102Cの比較解析

Language：Others  

Country：Other  

Fertility restorer gene Rf2 for Lead rice-type cytoplasmic male sterility of rice encodes mitochondrial glycine-rich protein.

Language：Others  

Country：Other  

イネミトコンドリア遺伝子のRNAエディティングに関わるPPRタンパク質MPR25

Language：Others  

Country：Other  

イネミトコンドリア遺伝子のnad5の転写後発現制御に関わるMPR25タンパク質

Language：Others  

Country：Other  

Oryza rufipogonに由来する３種類の稔性回復系統RT61C, RT98C, RT102Cの持つ稔性回復遺伝子の探索。

Language：Others  

Country：Other  

LD型細胞質雄性不稔イネに対する稔性回復遺伝子Rf2のマップベースクローニングおよび解析。

Language：Others  

Country：Other  

イネミトコンドリア遺伝子のRNAエディティングに関わるPPRタンパク質。

Language：Others  

Country：Other  

イネミトコンドリアにコードされる遺伝子転写産物の末端に関する研究。

Language：Others  

Country：Other  

イネミトコンドリア遺伝子発現制御に関わるMPR25タンパク質の機能解析。

Language：Others  

Country：Other  

イネLD型細胞質雄性不稔性に対する稔性回復遺伝子Rf2の単離および解析。

Language：Others  

Country：Other  

イネLD型細胞質雄性不稔性に対する稔性回復遺伝子Rf2はグリシンリッチタンパク質をコードする。

Language：Others  

Country：Other  

Oryza rufipogonに由来する細胞質雄性不稔性イネ３系統のミトコンドリアゲノム構造比較。

Language：Others  

Country：Other  

Oryza rufipogonに由来するRT61C, RT98C, RT102Cの持つ雄性不稔細胞質の比較解析。

Language：Others  

Country：Other  

Molecular comparison of fertility restorer genes, Rf1, Rf2 and Rf17, for cytoplasmic male sterility in rice.

Language：Others  

Venue：日本国 仙台   Country：Other  

光合成に影響を及ぼすイネミトコンドリアPentatricopeptide repeat MPR25の機能解析

Language：Others  

Venue：ハンガリー   Country：Other  

Molecular analysis of cytoplasmic male sterility and fertility restoration in rice.

Language：Others  

Venue：ハンガリー   Country：Other  

Comparison of fertility restoration mechanisms between BT and LD type cytoplasmic male sterile rice

Language：Others  

Venue：日本国 福井   Country：Other  

雄性不稔細胞質を持つ野生イネ８系統のミトコンドリアゲノム構造と稔性回復遺伝子の比較

Language：Others  

Venue：日本国   Country：Other  

雄性不稔細胞質を持つRT102Cにおける稔性回復遺伝子のマッピング

Language：Others  

Venue：日本国 福井   Country：Other  

光合成に影響を及ぼすイネミトコンドリアPPR MPR25の機能解析

Language：Others  

Venue：オーストラリア メルボルン   Country：Other  

Molecular analysis of partial fertility restorer gene, Rf2, for BT-type cytoplasmic male sterile rice.

Language：Others  

Venue：オーストラリア メルボルン   Country：Other  

Fertility restoration in three types of cytoplasmic male sterility in rice.

Language：Others  

Venue：京都   Country：Other  

Regulatory mechanisms of ORF79 translation by RF2 in cytoplasmic male sterile rice

Language：Others  

Country：Other  

雄性不稔性細胞質を持つ野生イネのミトコンドリアゲノム構造と稔性回復遺伝⼦の解析

Language：Others  

Venue：奈良   Country：Other  

細胞質雄性不稔イネRT98Aに対する稔性回復遺伝子のマッピング

Language：Others  

Venue：奈良   Country：Other  

ナガイモレクチンDB1遺伝子を導入したウンカ抵抗性粗飼料イネの作出

Language：Others  

Country：Other  

イネ開花期における斑点米カメムシの誘引物質合成に関わる遺伝子の解析

Language：Others  

Venue：奈良   Country：Other  

イネLD型細胞質雄性不稔系統における稔性回復因子RF2と相互作用するタンパク質の探索

Language：Others  

Venue：奈良   Country：Other  

WA型細胞質雄性不稔イネミトコンドリアにおけるorfB遺伝子の転写語制御に関する研究

Language：Others  

Country：Other  

雄性不稔細胞質を持つ3種類の野生イネにおけるRf1アリルの比較解析

Language：Others  

Country：Other  

植物ミトコンドリア遺伝子発現の分子基盤解明と育種への応用

Language：Others  

Venue：京都   Country：Other  

コメ産業の国際化を狙った新規ハイブリッドライス育種基盤の開発

Language：Others  

Venue：福岡   Country：Other  

イネにおけるミトコンドリア遺伝性の花粉発育不全とそれをレスキューする核遺伝子

Language：Others  

Country：Other  

LD型細胞質雄性不稔イネに対する稔性回復因子RF2と相互作用するタンパク質の解析

Language：Others  

Country：Other  

CMS系統RT98Aと稔性回復系統RT98CにおけるミトコンドリアmRNAの比較

Language：Others  

Country：Other  

イネ花粉発達にみるミトコンドリアと核のゲノム相関～ミトコンドリア遺伝子転写後制御に関わるRestorer of fertility-like pentatricopeptide repeat protein genes

Language：Others  

Venue：韓国   Country：Other  

Search for proteins that interact with RF2, a restorer of fertility of LD-type cytoplasmic male sterility in rice. 10th International Congress on Plant Molecular Biology

Language：Others  

Venue：ドイツ   Country：Other  

Identification of cytoplasmic male sterility associated-gene in RT102 cytoplasm derived from Oryza rufipogon.

Language：Others  

Venue：韓国   Country：Other  

Comparison of mitochondrial transcripts between RT98A cytoplasmic male sterility line and RT98C fertility restorer line.

Language：Others  

Venue：タイ   Country：Other  

Whole genomic sequencing of cytoplasmic male sterility (CMS) mitochondria derived from Oryza rufipogon to uncover CMS-associated genes.

Language：Others  

Venue：タイ   Country：Other  

Rice MPR25 encodes a pentatricopeptide repeat protein and is not only essential for RNA editing of nad5 transcripts in mitochondria but for chloroplast function.

Language：Others  

Venue：タイ   Country：Other  

Analysis of mitochondrial genes causing pollen abortion and restorer of fertility genes in Oryza rufipogon.

Language：Others  

Venue：タイ   Country：Other  

A yeast two hybrid screening to search for protein that interact with RF2, a restorer of fertility of LD-type cytoplasmic male sterility in rice.

Language：Others  

Country：Other  

細胞質によって稔性回復効果が異なる稔性回復因子RF2の機能解析

Language：Others  

Country：Other  

野生イネ（Oryza rufipogon）8系統における稔性回復遺伝子と細胞質雄性不稔性原因遺伝子atp6-orf79の比較

Language：Others  

Country：Other  

酵母ツーハイブリッド法とプルダウンアッセイによるイネLD型細胞質雄性不稔系統の稔性回復因子RF2と相互作用する因子の探索

Language：Others  

Country：Other  

T98型細胞質雄性不稔性イネにおけるCMS原因遺伝子候補orf113の同定

Language：Others  

Venue：アルゼンチン Rosario   Country：Other  

Comparison of fertility restoration system in two types of cytoplasmic male sterility in rice.

Language：Others  

Country：Other  

イネのミトコンドリア遺伝性の雄性生殖器官発育不全とそれをレスキューする核遺伝子

Language：Others  

Country：Other  

Struggle between mitochondria and nuclei exemplified by LD-type cytoplasmic male sterility in rice.

Language：Others  

Country：Other  

Struggle between mitochondria and nuclei exemplified by RT98-type cytoplasmic male sterility in rice.

Language：Others  

Country：Other  

Struggle between mitochondria and nuclei exemplified by RT102-type cytoplasmic male sterility in rice.

Language：Others  

Venue：札幌   Country：Other  

細胞質雄性不稔性イネRT02系統に依存するミトコンドリア原因遺伝子の探索

Language：Others  

Venue：札幌   Country：Other  

稔性回復因子RF2と相互作用を示すミトコンドリア局在因子の解析

Language：Others  

Venue：札幌   Country：Other  

orf79をミトコンドリアに持つ細胞質雄性不稔イネの比較解析

Language：Others  

Venue：札幌   Country：Other  

RT98型細胞質雄性不稔性原因遺伝子候補orf113の同定とリコンビナントタンパク質の毒性調査

Language：Others  

Venue：鹿児島   Country：Other  

開花期のイネから放出される香気成分の品種間差異

Language：Others  

Venue：鹿児島   Country：Other  

細胞質雄性不稔イネにおける稔性回復遺伝子Rf2の稔性回復効果の違いについての解析

Language：Others  

Venue：鹿児島   Country：Other  

イネRT98型CMS原因遺伝子orf113の稔性回復系統におけるRNAプロセッシング

Language：Others  

Venue：鹿児島   Country：Other  

イネRT102型CMS原因遺伝子orf352の稔性回復系統におけるRNAプロセッシング

Language：Others  

Venue：鹿児島   Country：Other  

イネLD型CMS系統における稔性回復因子RF2と相互作用するubiquitin domain containing proteinの解析

Language：Others  

Country：Other  

Correlative gene system between mitochondria and nuclei observed in RT98-type cytoplasmic male sterility in rice.

Language：Others  

Country：Other  

ミトコンドリアRNAの制御が花粉の運命を決定するミトコンドリアRNAの制御が花粉の運命を決定する

Language：Others  

Venue：マニラ   Country：Other  

Ubiquitin domain containing protein interacts with RF2, a restorer of fertility of LD-type cytoplasmic male sterility in rice.

Language：Others  

Venue：マニラ   Country：Other  

Determination of 5' and 3' ends of orf113 RNA associated with RT98-type cytoplasmic male sterility in rice.

Language：Others  

Venue：マニラ   Country：Other  

Comparative study on cytoplasmic male sterility-associated genes, orf79, orf113, orf352, derived from Oryza rufipogon.

Language：Others  

Country：Other  

ハイブリッドライス育種基盤の開発

Language：Others  

Country：Other  

植物のメス化：ミトコンドリアが花粉を殺す？

Language：Others  

Venue：都城   Country：Other  

イネRT98型細胞質雄性不稔性に対する稔性回復遺伝子のファインマッピング

Language：Others  

Venue：都城   Country：Other  

イネRT102型細胞質雄性不稔性に対する稔性回復遺伝子のファインマッピング

Language：Others  

Venue：都城   Country：Other  

WA型細胞質雄性不稔性イネに対する稔性回復遺伝子Rf4はPPRタンパク質をコードする

Language：Others  

Venue：都城   Country：Other  

LD型細胞質雄性不稔性イネに対する稔性回復因子RF2複合体の探索

Language：Others  

Country：Other  

イネ科葉緑体内包膜における蛋白質透過装置TICトランスロコンの同定
大阪大学との共同研究

Language：Others  

Venue：ポーランド Wroclaw   Country：Other  

Mitochondrial genotype determines fate of pollen development in cytoplasmic male sterility

Language：Others  

Country：Other  

イネRT98型細胞質雄性不稔系統と可稔系統の葯遺伝子発現比較解析

Language：Others  

Country：Other  

WA型細胞質雄性不稔イネの稔性回復因子RF4はミトコンドリアにおいてWA352 RNAの分解に関与する

Language：Others  

Venue：新潟   Country：Other  

野生イネの第10 染色体に存在するBT型細胞質雄性不稔イネに対する稔性回復遺伝子の解析

Language：Others  

Venue：新潟   Country：Other  

世界のイネ・コアコレクションにおける柱頭露出性の評価

Language：Others  

Venue：新潟   Country：Other  

RNA-seqで細胞質雄性不稔系統RT98Aと維持系統T65で発現が異なる遺伝子の解析

Language：Others  

Venue：新潟   Country：Other  

CW型細胞質を利用したIR24の細胞質雄性不稔系統の作出

Language：Others  

Country：Other  

細胞質雄性不稔イネへの変異源処理による稔性回復復帰変異体スクリーニングの試み

Language：Others  

Country：Other  

世界のイネコアコレクションに由来する新規細胞質雄性不稔系統作出の試み

Language：Others  

Country：Other  

スリランカ在来品種KALUHEENATIに由来する細胞質雄性不稔性とその稔性回復遺伝子の研究

Language：Others  

Country：Other  

イネ開花期における香気成分の合成・放出に関わる遺伝子の解析

Language：Others  

Venue：日本国   Country：Other  

野生イネOryza rufipogon2系統における第10染色体Rf1領域の解析

Language：Others  

Venue：日本国   Country：Other  

Genetic analysis of fertility restorer locus o chromosome 10 derived from a wild rice.

Language：Others  

Venue：鳥取   Country：Other  

世界のイネ・コアコレクションに由来する新規細胞質雄性不稔系統の育成

Language：Others  

Venue：鳥取   Country：Other  

orf79を保有する様々なイネにおけるorf79発現制御の比較解析

Language：Others  

Venue：鳥取   Country：Other  

Boro型細胞質雄性不稔性イネのミトコンドリアゲノムの解読

Language：Japanese  

Country：Other  

Language：Japanese  

Country：Other  

Language：English  

Country：Other  

Comparative analysis of cytoplasmic male sterility developed from various rice

Language：Japanese  

Country：Other  

Language：Japanese  

Country：Other  

Language：Japanese  

Country：Other  

Language：Japanese  

Country：Other  

Language：Japanese  

Country：Other  

Language：Japanese  

Country：Other  

Language：Japanese  

Country：Other  

Language：Japanese  

Country：Other  

Language：Japanese  

Country：Other  

Language：Japanese  

Country：Other  

Language：Japanese  

Country：Other  

Language：Japanese  

Country：Other  

Language：English  

Country：Other  

Mitochondrial genome modification using mitochondria-targeted TALEN

Language：Japanese  

Country：Other  

Language：Japanese  

Country：Other  

Language：Japanese  

Country：Other  

Language：Japanese  

ナガイモレクチンDB1遺伝子を導入したウンカ抵抗性粗飼料用イネの作出

Language：English  

An allopolyploid is an individual having two or more complete sets of chromosomes derived from different species. Generation of allopolyploids might be rare because of the need to overcome limitations such as co-existing populations of parental lines, overcoming hybrid incompatibility, gametic non-reduction, and the requirement for chromosome doubling. However, allopolyploids are widely observed among plant species, so allopolyploids have succeeded in overcoming these limitations and may have a selective advantage. As techniques for making allopolyploids are developed, we can compare transcription, genome organization, and epigenetic modifications between synthesized allopolyploids and their direct parental lines or between several generations of allopolyploids. It has been suggested that divergence of transcription caused either genetically or epigenetically, which can contribute to plant phenotype, is important for the adaptation of allopolyploids.

DOI： 10.3390/ijms13078696

Language：English  

DOI： 10.1111/j.1365-313X.2011.04678.x

Language：Japanese  

細胞質雄性不稔植物の稔性回復遺伝子の正体とは？ PPRタンパク質によるミトコンドリア遺伝子の発現制

磐城高校1年生が、農学部内の研究室見学を行った際、環境適応生物工学分野の案内を行った。

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フンボルト大学において、セミナーを行った。

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ウルム大学において、セミナーを行った。

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磐城高校1年生が、農学部内の研究室見学を行った際、環境適応生物工学分野の案内を行った。

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第2期発展コース（実験コース）で「お米一粒で品種判別せよ！」を担当

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第50回日本植物生理学会年会のサテライトミーティングとして開催されたオルガネラワークショップにおいて、「細胞質雄性不稔イネの解析から見えてきたもの」という内容で、講演した。この講演は、招待講演である。

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九州大学高等研究機構の中村崇裕准教授の研究室で「BT型細胞質雄性不稔イネの稔性回復メカニズム」というタイトルでセミナーを行った。

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