Updated on 2024/10/02

Information

 

写真a

 
YAMAGAMI TAKESHI
 
Organization
Faculty of Agriculture Department of Bioscience and Biotechnology Assistant Professor
School of Agriculture Department of Bioresource and Bioenvironment(Concurrent)
Graduate School of Bioresource and Bioenvironmental Sciences Department of Bioscience and Biotechnology(Concurrent)
Title
Assistant Professor
Contact information
メールアドレス
Profile
アーキア(古細菌)のDNA複製修復機構の解明を行っている。トポイソメラーゼの研究を行っている。
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Research Areas

  • Life Science / Applied biochemistry

Degree

  • Ph.D (Agricultural science)

Research History

  • - Research Associate, Faculty of Agriculture,

    1992

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  • - 九州大学農学研究科助手

    1992

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  • 社団法人岡山県農業開発研究所

    社団法人岡山県農業開発研究所

  • Kyushu University

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Education

  • Kyushu University

    - 1991

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    Country: Japan

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  • Kyushu University   Graduate School, Division of Agriculture   Genetic Resources Technology

    - 1991

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  • Okayama University

    - 1988

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    Country: Japan

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  • Okayama University   Graduate School, Division of Agriculture   Agricultural chemistry

    - 1988

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  • Okayama University   Faculty of Agriculture

    - 1986

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    Country: Japan

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  • Okayama University   Faculty of Agriculture

    - 1986

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Research Interests・Research Keywords

  • Research theme:たんぱく質化学

    Keyword:たんぱく質化学

    Research period: 2024

  • Research theme:Protein Chemistry

    Keyword:Protein Chemistry

    Research period: 2024

  • Research theme:DNA polymerase

    Keyword:DNA polymerase

    Research period: 2023.6

  • Research theme:topoisomerase

    Keyword:topoisomerase

    Research period: 2022.6

  • Research theme:Replisome

    Keyword:replisome

    Research period: 2020.11

  • Research theme:Studies on DNA polymerases from Archaea

    Keyword:DNA polymerase

    Research period: 2008.4 - 2010.3

  • Research theme:Creation of DNA polymerase using metagenome information

    Keyword:DNA polymerase

    Research period: 2004.4

  • Research theme:studies on ACS synthase from Arabidopsis

    Keyword:Arabidopsis, ethylene, ACC synthase

    Research period: 2002.3 - 2002.2

  • Research theme:Studies on DNA replication of Archaea

    Keyword:DNA replication

    Research period: 2002.3

  • Research theme:structure-function relationship of plant chitinases

    Keyword:chitinase

    Research period: 1989.4 - 2000.2

Papers

  • A longer finger-subdomain of family A DNA polymerases found by metagenomic analysis strengthens DNA binding and primer extension abilities. Reviewed International journal

    Yamagami, T., Matsukawa, H., Tsunekawa, S., Kawarabayasi, Y., Ishino, S., and Ishino, Y.

    Gene   2015.10

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016

  • Mutant Taq DNA polymerases with improved elongation ability as a useful reagent for genetic engineering. Reviewed International journal

    Yamagami, T., Ishino, S., Kawarabayasi, Y., and Ishino, Y.

    Front Microbiol.   2014.9

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    Language:English   Publishing type:Research paper (scientific journal)  

  • Biochemical diversity among the 1-amino-cyclopropane-1-carboxylic acid synthase isozymes encoded by Arabidopsis gene family Reviewed International journal

    Yamagami T., A. Tsuchisaka, K. Yamada, W. F. Haddon, L. A. Harden, and A. Theologis

    J. Biol. Chem.   2003.12

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  • Positions of disulfide bonds in rye (Secale cereale) seed chitinase-a Reviewed

    Yamagami, T., G. Funatsu, and M. Ishiguro

    Biosci. Biotechnol. Biochem.   64 ( 6 )   1313 - 1316   2000.1

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1271/bbb.64.1313

  • Complete amino acid sequence of chitinase-1 and -2 from bulbs of genus Tulipa, Reviewed

    Yamagami, T. and M. Ishiguro

    Biosci. Biotechnol. Biochem.   62 ( 6 )   1253 - 1257   1998.1

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1271/bbb.62.1253

  • Family D DNA polymerase interacts with GINS to promote CMG-helicase in the archaeal replisome

    Oki, K; Nagata, M; Yamagami, T; Numata, T; Ishino, S; Oyama, T; Ishino, Y

    NUCLEIC ACIDS RESEARCH   50 ( 7 )   3601 - 3615   2022.4   ISSN:0305-1048 eISSN:1362-4962

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    Language:English   Publisher:Nucleic Acids Research  

    Genomic DNA replication requires replisome assembly. We show here the molecular mechanism by which CMG (GAN-MCM-GINS)-like helicase cooperates with the family D DNA polymerase (PolD) in Thermococcus kodakarensis. The archaeal GINS contains two Gins51 subunits, the C-terminal domain of which (Gins51C) interacts with GAN. We discovered that Gins51C also interacts with the N-terminal domain of PolD's DP1 subunit (DP1N) to connect two PolDs in GINS. The two replicases in the replisome should be responsible for leading-and lagging-strand synthesis, respectively. Crystal structure analysis of the DP1N-Gins51C-GAN ternary complex was provided to understand the structural basis of the connection between the helicase and DNA polymerase. Site-directed mutagenesis analysis supported the interaction mode obtained from the crystal structure. Furthermore, the assembly of helicase and replicase identified in this study is also conserved in Eukarya. PolD enhances the parental strand unwinding via stimulation of ATPase activity of the CMG-complex. This is the first evidence of the functional connection between replicase and helicase in Archaea. These results suggest that the direct interaction of PolD with CMG-helicase is critical for synchronizing strand unwinding and nascent strand synthesis and possibly provide a functional machinery for the effective progression of the replication fork.

    DOI: 10.1093/nar/gkab799

    Web of Science

    Scopus

    PubMed

  • Genetic and Biochemical Characterizations of aLhr1 Helicase in the Thermophilic Crenarchaeon Sulfolobus acidocaldarius. Reviewed International journal

    Suzuki, S., Kurosawa, N., Yamagami, T., Matsumoto, S., Numata, T., Ishino, S., and Ishino, Y.

    Catalysts   12   2022.4

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    Language:Japanese   Publishing type:Research paper (scientific journal)  

  • Genetic and biochemical characterizations of aLhr1 helicase in the thermophilic crenarchaeon sulfolobus acidocaldarius Reviewed

    Shoji Suzuki, Norio Kurosawa, Takeshi Yamagami, Shunsuke Matsumoto, Tomoyuki Numata, Sonoko Ishino, Yoshizumi Ishino

    Catalysts   12 ( 1 )   2022.1   eISSN:2073-4344

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    Homologous recombination (HR) refers to the process of information exchange between homologous DNA duplexes and is composed of four main steps: end resection, strand invasion and formation of a Holliday junction (HJ), branch migration, and resolution of the HJ. Within each step of HR in Archaea, the helicase-promoting branch migration is not fully understood. Previous biochemical studies identified three candidates for archaeal helicase promoting branch migration in vitro: Hjm/Hel308, PINA, and archaeal long helicase related (aLhr) 2. However, there is no direct evidence of their involvement in HR in vivo. Here, we identified a novel helicase encoded by Saci_0814, isolated from the thermophilic crenarchaeon Sulfolobus acidocaldarius; the helicase dissociated a synthetic HJ. Notably, HR frequency in the Saci_0814-deleted strain was lower than that of the parent strain (5-fold decrease), indicating that Saci_0814 may be involved in HR in vivo. Saci_0814 is classified as an aLhr1 under superfamily 2 helicases; its homologs are conserved among Archaea. Purified protein produced in Escherichia coli showed branch migration activity in vitro. Based on both genetic and biochemical evidence, we suggest that aLhr1 is involved in HR and may function as a branch migration helicase in S. acidocaldarius.

    DOI: 10.3390/catal12010034

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  • Family D DNA polymerase interacts with GINS to promote CMG-helicase in the archaeal replisome. Reviewed International journal

    Oki, K., Nagata, M., Yamagami, T., Numata, T., Ishino, S., Oyama, O., and Ishino, Y.

    Nucleic Acids Res.   49   2021.6

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    Language:Japanese   Publishing type:Research paper (scientific journal)  

  • DNA polymerase D temporarily connects primase to the CMG-like helicase before interacting with proliferating cell nuclear antigen. Reviewed International journal

    Oki, K., Yamagami, T., Nagata, M., Mayanagi, K., Shirai T., Adachi, N., Numata, T., Ishino, S., and Ishino, Y.

    Nucleic Acids Res.   49   4599 - 4612   2021.4

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    Language:Japanese   Publishing type:Research paper (scientific journal)  

  • Two conformations of DNA polymerase D-PCNA-DNA, an archaeal replisome complex, revealed by cryo-electron microscopy Invited Reviewed International journal

    Mayanagi, K., Oki, K., Miyazaki, N., Ishino, S., Yamagami, T., Morikawa, K., Iwasaki, K., Kohda, D., Shirai, T., and Ishino, Y

    BMC Biol.   2020.11

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  • Two Family B DNA Polymerases From Aeropyrum pernix, Based on Revised Translational Frames Invited Reviewed International journal

    Daimon, K., Ishino, S., Imai, N., Nagumo, S., Yamagami, T., Matsukawa, H. and Ishino, Y.

    Frontiers in Molecular Biosciences   5   3389   2018.11

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  • Replication protein A complex in Thermococcus kodakarensis interacts with DNA polymerases and helps their effective strand synthesis Invited Reviewed International journal

    Nagata, M., Ishino, S., Yamagami, T., and Ishino, Y.

    Biosci. Biotechnol. Biochem.   83   695 - 704   2018.11

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  • Elucidating functions of DP1 and DP2 subunits from the Thermococcus kodakarensis family D DNA polymerase Invited Reviewed International journal

    Takashima, N., Ishino, S., Oki, K., Takafuji, M., Yamagami, T., Matsuo, R., Mayanagi, K., and Ishino, Y.

    Extremophiles   23   161 - 172   2018.11

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  • Possible function of the second RecJ-like protein in stalled replication fork repair by interacting with Hef Invited Reviewed International journal

    Nagata, M., Ishino, S., Yamagami, T., Simons, J-R., Kanai, T., Atomi, H., and Ishino, Y.

    Sci Rep.   7   16949   2017.11

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  • The Cdc45/RecJ-like protein forms a complex with GINS and MCM, and is important for DNA replication in Thermococcus kodakarensis Invited Reviewed International journal

    Nagata, M., Ishino, S., Yamagami, T., Ogino, H., Simons, J-R., Kanai, T., Atomi, H., and Ishino, Y.

    Nucleic Acids Res.   45   10693 - 10705   2017.11

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  • Identification of a mismatch-specific endonuclease in hyperthermophilic Archaea Invited Reviewed International journal

    Ishino, S., Nishi, Y., Oda, S., Uemori, T., Sagara, T., Takatsu, N., Yamagami, T., Shirai, T., and Ishino, Y.

    Nucleic Acids Res.   44   2977 - 2986   2016.11

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  • Atomic structure of an archaeal GAN suggests its dual roles as an exonuclease in DNA repair and a CMG component in DNA replication Invited Reviewed International journal

    Nucleic Acids, Res   44   9509 - 9517   2016.11

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  • PCNA is involved in the EndoQ-mediated DNA repair process in Thermococcales Invited Reviewed International journal

    Shiraishi, M., Ishino, S., Yoshida, K., Yamagami, T., Cann, I., and Ishino, Y.

    Sci. Rep.   6   25532   2016.11

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  • A novel endonuclease that may be responsible for damaged DNA base repair in Pyrococcus furious Invited Reviewed International journal

    Shiraishi, M., Ishino, S., Yamagami, T., Egashira, Y., Kiyonari, S., and Ishino, Y.

    Nucleic Acids Res.   43   2858 - 2863   2015.11

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  • EndoQ and EndoV work individually for damaged DNA base repair in Pyrococcus furious Invited Reviewed International journal

    Ishino, S, Makita, N, Shiraishi, M, Yamagami, T, and Ishino, Y.

    Biochimie   118   264 - 269   2015.11

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  • Multiple interactions of the intrinsically disordered region between the helicase and the nuclease domains of the archaeal Hef protein Invited Reviewed International journal

    Ishino, S., Yamagami, T., Kitamura, M., Kodera, N., Mori, T., Sugiyama, S., Ando, T., Goda, N., Tenno, T,, Hiroaki, H., and Ishino, Y

    J. Biol. Chem.   289   21627 - 21636   2014.11

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  • The OsGEN-L protein from Oryza sativa possesses Holliday junction resolvase activity as well as 5'-flap endonuclease activity Invited Reviewed

    Yang, Y., Ishino, S., Yamagami, T., Kumamaru, T., Satoh, H., and Ishino, Y.

    J. Biochem.   151   317 - 327   2012.11

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  • Comparative analyses of the two PCNAs from the hyperthermophilic archaeon, Thermococcus kodakarensis Reviewed International journal

    Kuba, Y., Ishino, S., Yamagami, T., Tokuhara, M., Kanai, T., Fujikane, R.,Daiyasu., Atomi, H., Ishino, Y.

    Genes Cells   17   923 - 937   2012.11

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  • Cdc6/Orc1 from Pyrococcus furiosus may act as the origin recognition protein and Mcm helicase recruiter Invited Reviewed International journal

    Akita, M., Adachi, A, Takemura, K., Yamagami, T., Matsunaga, F., and Ishino, Y.

    Genes Cells   15   537 - 552   2010.11

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  • A useful strategy to construct DNA polymerases with different properties by using genetic resources from environmental DNA Reviewed

    Matsukawa, H., Yamagami, T.,Kawarabayasi, Y., Miyashita, Y., Takahashi, M., and Ishino, Y.

    Genes Genet. Syst.   84   3 - 13   2009.11

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  • Localized melting of duplex DNA by Cdc6/Orc1 at the DNA replication origin in the hyperthermophilic archaeon Pyrococcus furiosus Invited Reviewed International journal

    Matsunaga, F., Takemura, K., Akita, M., Adachi, A., Yamagami, T., and Ishino, Y.

    Extremophiles   14   21 - 31   2009.1

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  • Molecular cloning, functional expression, and mutagenesis of cDNA encoding class I chitinase from rye (Secale cereale) Reviewed

    Ohnuma., T., Taira, T., Yamagami, T., and Ishiguro, M.

    Biosci. Biotech. Biochem.   68 ( 3 )   324 - 332   2004.3

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  • Mutational analysis of amino acid residues involved in catalytic activity of a family 18 chitinase from tulip bulbs Reviewed

    Suzukawa K., T. Yamagami, T. Ohnuma, H. Hirakawa, S. Kuhara, Y, Aso, and M. Ishiguro

    Biosci. Biotechnol. Biochem.   67 ( 2 )   341 - 346   2003.1

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  • Efficient production of recombinant plant chitinase with Novagen's E. coli Origami(DE3) strain International journal

    Ohnuma T., M. Yagi, T. Taira, T. Yamagami, and M. Ishiguro,

    inNovations   2003.1

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  • Molecular cloning, functional expression, and mutagenesis of cDNA encoding rye seed chitinase-c Reviewed

    Ohnuma T., M. Yagi, T. Yamagami, T. Taira, Y. Aso, and M. Ishiguro,

    Biosci. Biotechnol. Biochem.   66 ( 2 )   277 - 284   2002.1

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  • Antifungal activity of rye seed chitinases: Different binding manner of class I and class II chitinases to the fungal cell walls Reviewed

    Taira T., T. Ohnuma, T. Yamagami, Y. Aso, M. Ishiguro, and M. Ishihara

    Biosci. Biotechnol. Biochem.   66 ( 5 )   970 - 977   2002.1

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  • Localization, accumulation, and antifungal activity of chitinases in rye seed, Reviewed

    Taira T., T. Yamagami, Y. Aso, M. Ishiguro, and M. Ishihara

    Biosci. Biotechnol. Biochem.   65 ( 12 )   2710 - 2718   2001.1

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    DOI: 10.1271/bbb.65.2710

  • Modification of tryptophan residues of plant class III chitinases involved in enzyme activity

    Ishiguro M., T. Yamagami, M. Tanigawa, K. Tsutsumi, G. Funatsu, T. Ohnuma, and Y. Aso,

    J. Fac. Agr. Kyushu Univ.   46 ( 1 )   243 - 250   2001.1

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  • Cloning, sequencing, and expression of the tulip bulb chitinase-1 cDNA Reviewed

    Yamagami, T., K. Tsutsumi, and M. Ishiguro

    Biosci. Biotechnol. Biochem.   64 ( 7 )   1394 - 1401   2000.1

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1271/bbb.64.1394

  • Complete amino acid sequence of chitinase-A from leaves of pokeweed (Phytolacca americana) Invited Reviewed International journal

    Yamagami T, Tanigawa M, Ishiguro M, Funatsu G.

    Biosci Biotechnol Biochem.   62 ( 4 )   825 - 828   1998.4

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  • Identification of the aspartic acid residue located at or near substrate-binding site of rye seed chitinase-c Reviewed

    Yamagami, T. and G. Funatsu

    Biosci. Biotechnol. Biochem.   62 ( 2 )   383 - 385   1998.1

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    DOI: 10.1271/bbb.62.383

  • Isolation and characterization of chitinase isoforms from the bulbs of four species of the genus tulipa Reviewed

    Yamagami, T., T. Taira, Y. Mine, Y. Aso and M. Ishiguro

    Biosci. Biotechnol. Biochem.   62 ( 3 )   584 - 587   1998.1

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1271/bbb.62.584

  • Complete amino acid sequence of chitinase-a from bulbs of gladiolus (Gladiolus gandavenis), Reviewed

    Yamagami, T., Y. Mine and M. Ishiguro

    Biosci. Biotechnol. Biochem.   62 ( 2 )   386 - 389   1998.1

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    DOI: 10.1271/bbb.62.386

  • Purification and characterization of two chitinase isoforms from the bulbs of gladiolus (Gladiolus gandavensis) Invited Reviewed

    Yamagami, T., Mine, Y., Aso, Y., and Ishiguro, M.

    Biosci. Biotech. Biochem.   61 ( 11 )   2140 - 2142   1997.11

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  • Involvements of Trp23 in the Chitin-binding and of Trp131 in the Chitinase Activity of Rye Seed Chitinase-a Invited Reviewed

    Yamagami T, Funatsu G.

    Biosci Biotechnol Biochem.   61 ( 11 )   1819 - 1825   1997.6

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  • Limited proteolysis and reduction-carboxymethylation of rye seed chitinase-a: role of the chitin-binding domain in its chitinase action Invited Reviewed

    Yamagami T, Funatsu G.

    Biosci Biotechnol Biochem.   60 ( 7 )   1081 - 1086   1996.7

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  • Identification of the tryptophan residue located at the substrate-binding site of rye seed chitinase-c Invited Reviewed

    Yamagami T, Funatsu G.

    Biosci Biotechnol Biochem.   59   1076 - 1081   1995.10

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  • The complete amino acid sequence of chitinase-B from the leaves of pokeweed (Phytolacca americana) Invited Reviewed

    Tanigawa M, Yamagami T, Funatsu G.

    Biosci Biotechnol Biochem.   59 ( 5 )   841 - 847   1995.5

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  • Purification and characterization of two chitinases from the leaves of pokeweed (Phytolacca americana) Invited Reviewed

    Ohta M, Yamagami T, Funatsu G.

    Biosci Biotechnol Biochem.   59 ( 4 )   656 - 661   1995.4

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  • Partial purification and characterzation of DOPA quinone imine conversion factor from larval hemolymph of the solkworm, Bambyx mori Invited Reviewed

    Aso, Y., Yamamoto, K., Yoshinaga, T., Yamamoto, H., and Yamagami, T.

    Biosci. Biotech. Biochem.   59 ( 2 )   277 - 281   1995.2

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  • The complete amino acid sequence of chitinase-a from the seeds of rye (Secale cereal) Invited Reviewed

    Yamagami T, Funatsu G.

    Biosci Biotechnol Biochem.   58 ( 2 )   322 - 329   1994.2

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  • The complete amino acid sequence of chitinase-c from the seeds of rye (Secale cereal) Invited Reviewed

    Yamagami T, Funatsu G.

    Biosci Biotechnol Biochem.   57 ( 11 )   1854 - 1861   1993.11

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  • Purification and some properties of three chitinases from the seeds of rye (Secale cereale) Invited Reviewed

    Yamagami T, Funatsu G.

    Biosci Biotechnol Biochem.   57 ( 4 )   643 - 647   1993.4

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  • Helical configuration of lutein aggregate dispersed in liposomes of phosphatidyl choline and digalactocyldiglyceride Invited Reviewed

    Takagi, S., Yamagami, T., Takeda, K., and Takagi, T.

    Agric. Biol. Chem.   51   1567 - 1572   1987.10

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Presentations

  • Thermococcus kodakarensis由来ファミリーD DNAポリメラーゼの調整法の改良とその生化学的解析

    山上 健、沖啓輔、高島夏希、高藤三加、真柳浩太、石野園子、石野良純

    日本分子生物学会  2019.12 

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    Event date: 2019.12

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Country:Japan  

    ファミリーD DNAポリメラーゼ (PolD) は、アーキア特有の酵素であり、クレンアーキオタ以外のサブドメインでホモログ遺伝子が見つかっている。アーキア細胞内では、PolDは、ファミリーB DNAポリメラーゼ (PolB) と共に必須の複製酵素であると予想されてきたが、近年、一部のユリアーキオタにおいて、polB破壊株が得られたという報告が相次いだことから、アーキアの複製酵素としての可能性がより高まった。我々は、超好熱性アーキアThermococcus kodakarensisのPolDについて、その構造と機能を詳細に理解するために研究を進めている。PolDおよびその構成タンパク質であるDP1、DP2について、精製法の検討を行ってきた。すでに報告している方法 (Extremophiles, 23, 161 (2019))をさらに改良し、基材との弱い相互作用を期待し、親水性ビニルポリマーを基材としたTOYOPEARL Phenyl-650S(疎水性)、ヘパリン(親和性)および親水性ポリマーを基材としたYMC BioPro SmartSep Q(陰イオン交換)カラムを用いて高純度にタンパク質の精製を行った。その結果、疎水クロマトでは、DP1-DP2複合体がきれいなピークとして溶出し、陰イオン交換クロマト後のサンプルで、より高純度に精製されていることがSDS-PAGEで確認された。次に、DP2の各ドメインタンパク質を同様に精製し、DP1との結合およびDNAとの結合を調べた結果、DP1とDP2-C-terminal domain (-CTD)が結合し、その複合体はprimed-DNAに結合することがわかった。現在、複合体の結晶化を試みるとともに、電子顕微鏡単粒子解析によって、PolDの全体構造およびPolDがDNA上で作用する際の構造モデル構築を目指している。

  • クレンアーキオータクレンアーキオータ門において機能的にヘリカーゼとプライマーゼプライマーゼを連結するタンパク質の検索

    山上 健、瀬戸口勇登、沖 啓輔、石野園子、石野良純

    日本分子生物学会  2018.12 

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    Event date: 2018.12

    Language:Japanese  

    Venue:横浜市   Country:Japan  

  • 温泉土壌メタゲノム情報を利用した新規DNAポリメラーゼの創製

    山上 健

    日本分子生物学会  2008.12 

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    Event date: 2008.12

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:横浜市   Country:Japan  

  • 温泉土壌メタゲノム情報を利用したTaqポリメラーゼの創製

    山上 健、興梠聖哉、田島秀二、河原林裕、石野良純

    日本分子生物学会  2007.12 

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    Presentation type:Symposium, workshop panel (public)  

    Venue:横浜市   Country:Japan  

  • チューリップ (Tulipa tarda) 球根キチナーゼ(TBC-2)の全アミノ酸配列

    山上 健, 石黒正恒

    日本農芸化学会大会  1998.4 

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    Presentation type:Oral presentation (general)  

    Venue:名古屋   Country:Japan  

  • チューリップ球根キチナーゼ(TBC-1)のトリプトファン残基の化学修飾

    堤 一貴, 山上 健, 麻生陽一, 石黒正恒

    日本農芸化学会大会  1998.4 

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    Presentation type:Oral presentation (general)  

    Venue:名古屋   Country:Japan  

  • 植物クラスIキチナーゼに関する研究(その1):Cys-richドメインに対する抗体の性質

    平良東紀, 山上 健, 麻生陽一, 石黒正恒

    日本農芸化学会大会  1998.4 

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    Presentation type:Oral presentation (general)  

    Venue:名古屋   Country:Japan  

  • ライ麦種子キチナーゼの局在性と種子登熟過程における変動

    平良東紀,山上 健,麻生陽一,石黒正恒

    日本農芸化学会大会  1999.3 

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    Presentation type:Oral presentation (general)  

    Venue:福岡   Country:Japan  

  • チューリップ球根キチナーゼの部位特異的変異

    鈴川啓輔、大沼貴之、山上 健、石黒正恒

    日本農芸化学会西日本支部大会  2000.9 

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    Presentation type:Oral presentation (general)  

    Venue:沖縄   Country:Japan  

  • チューリップ球根キチナーゼ遺伝子のクローニング,発現および変異

    鈴川啓輔,山上 健,堤 一貴,石黒正恒

    日本農芸化学会大会  2000.4 

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    Presentation type:Oral presentation (general)  

    Venue:東京   Country:Japan  

  • ライ麦キチナーゼ遺伝子のクローニング

    大沼貴之、山上 健、麻生陽一、石黒正恒

    日本農芸化学会西日本支部大会  2000.9 

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    Presentation type:Oral presentation (general)  

    Venue:沖縄   Country:Japan  

  • チューリップ球根キチナーゼの部位特異的変異

    鈴川啓輔,大沼貴之,山上 健,麻生陽一,石黒正恒

    日本農芸化学会大会  2001.3 

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    Venue:東京   Country:Japan  

  • ライ麦種子キチナーゼの抗カビ活性

    平良東記、大沼貴之、山上 健、麻生陽一、石原昌信、石黒正恒

    日本農芸化学会関西、西日本、中四国合同大会  2001.10 

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    Presentation type:Oral presentation (general)  

    Venue:岡山   Country:Japan  

  • ライ麦種子キチナーゼ遺伝子のクローニングと大腸菌による発現

    大沼貴之,八木美佳子,山上 健,麻生陽一,石黒正恒

    日本農芸化学会大会  2001.3 

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    Presentation type:Oral presentation (general)  

    Venue:東京   Country:Japan  

  • ライ麦種子クラスIキチナーゼの真菌細胞壁への結合と抗真菌活性

    平良 東紀,大沼 貴之,山上 健,麻生 陽一,石原 昌信,石黒 正恒

    日本農芸化学会大会  2002.3 

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    Presentation type:Oral presentation (general)  

    Venue:仙台   Country:Japan  

  • ライ麦種子クラスI型キチナーゼ(RSC-a)の大腸菌での発現および組換え型RSC-aの性質

    日本農芸化学会大会  2002.3 

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    Presentation type:Oral presentation (general)  

    Venue:仙台   Country:Japan  

  • DNA複製因子PCNA-RFC間の機能的相互作用解析

    服部辰彦、山上 健、麻生陽一、石野園子、大山拓次、森川耿右、石野良純

    日本分子生物学会年会  2003.12 

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    Presentation type:Symposium, workshop panel (public)  

    Venue:神戸   Country:Japan  

  • DNA複製因子PCNA-RFC間の機能的相互作用解析

    服部辰彦、山上 健、麻生陽一、石野良純

    日本農芸化学会関西、西日本、中四国支部合同大会  2003.9 

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    Presentation type:Oral presentation (general)  

    Venue:鹿児島   Country:Japan  

  • 新規DNA複製関連因子の探索

    吉永亜耶、山上 健、麻生陽一、石野良純

    日本農芸化学会西日本支部大会  2003.9 

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    Presentation type:Oral presentation (general)  

    Venue:鹿児島   Country:Japan  

  • 大腸菌および酵母Two-hybridシステムを用いた古細菌DNA複製開始複合体と相互作用するタンパク質因子の検索

    山上 健、吉永亜耶、石野良純

    日本分子生物学会年会  2003.12 

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    Presentation type:Symposium, workshop panel (public)  

    Venue:神戸   Country:Japan  

  • 超好熱性アーキアPyrococcus furiosus由来DNAリガーゼの性質解析

    高山耕平、藤兼亮輔、山上 健、麻生陽一、石野良純

    日本農芸化学会関西、西日本、中四国支部合同大会  2003.9 

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    Presentation type:Oral presentation (general)  

    Venue:鹿児島   Country:Japan  

  • 超好熱性古細菌Pyrococcus furiosus由来family D DNA polymeraseの機能及び構造解析

    當利和夫、藤兼亮輔、山上 健、品川日出夫、石野良純

    日本分子生物学会年会  2003.12 

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    Presentation type:Symposium, workshop panel (public)  

    Venue:神戸   Country:Japan  

  • 温泉土壌メタゲノム情報を利用したTaqポリメラーゼの創製

    山上 健、興梠聖哉、田島秀二、河原林 裕、石野良純

    日本分子生物学会  2007.12 

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    Presentation type:Symposium, workshop panel (public)  

    Venue:横浜市   Country:Japan  

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MISC

  • メタゲノムを利用した新規DNA合成酵素の創製

    @石野良純 、@山上 健、#松川博昭、#鬼塚尚子、#鍋 健吾、#興梠聖哉

    環境バイオテクノロジー学会誌, 7, 87-92.   2007.10

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    Language:Japanese   Publishing type:Article, review, commentary, editorial, etc. (scientific journal)  

Industrial property rights

Patent   Number of applications: 7   Number of registrations: 1
Utility model   Number of applications: 0   Number of registrations: 0
Design   Number of applications: 0   Number of registrations: 0
Trademark   Number of applications: 0   Number of registrations: 0

Professional Memberships

  • 日本生化学会

  • 日本農芸化学会

  • 日本分子生物学会

  • 日本蛋白質科学会

  • 日本農芸化学会

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  • 日本蛋白質科学会

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  • 日本生化学会

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  • 日本分子生物学会(国内)

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Academic Activities

  • 座長(Chairmanship)

    日本農芸化学会西日本支部中大会  ( 長崎大学 ) 2008.9

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    Type:Competition, symposium, etc. 

  • Screening of academic papers

    Role(s): Peer review

    2008

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    Type:Peer review 

    Number of peer-reviewed articles in foreign language journals:3

    Number of peer-reviewed articles in Japanese journals:0

    Proceedings of International Conference Number of peer-reviewed papers:0

    Proceedings of domestic conference Number of peer-reviewed papers:0

  • 座長(Chairmanship)

    日本農芸化学会西日本支部中四国支部合同大会  ( 山口市 ) 2007.9 - 2016.4

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    Type:Competition, symposium, etc. 

  • Screening of academic papers

    Role(s): Peer review

    2006

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    Type:Peer review 

    Number of peer-reviewed articles in foreign language journals:2

    Number of peer-reviewed articles in Japanese journals:0

    Proceedings of International Conference Number of peer-reviewed papers:0

    Proceedings of domestic conference Number of peer-reviewed papers:0

  • 福岡地区世話人

    第29回蛋白質の構造と機能に関する九州シンポジウム  ( 玄海ロイヤルホテル ) 2005.9 - 2016.4

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    Type:Competition, symposium, etc. 

    Number of participants:70

  • 座長(Chairmanship)

    日本農芸化学会西日本支部、中四国支部鹿児島合同大会  ( 鹿児島 ) 2003.10 - Present

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    Type:Competition, symposium, etc. 

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Class subject

  • 分子細胞生物学

    2024.4 - 2024.9   First semester

  • 分子細胞生物学

    2023.4 - 2023.9   First semester

  • 分子細胞生物学

    2022.4 - 2022.9   First semester

  • 分子細胞生物学

    2021.4 - 2021.9   First semester

  • 分子細胞生物学

    2020.4 - 2020.9   First semester

  • 分子細胞生物学

    2019.4 - 2019.9   First semester

  • 実地見学(応用生命化学分野)

    2019.4 - 2019.9   First semester

  • 分子細胞生物学

    2018.4 - 2018.9   First semester

  • 現地見学

    2018.4 - 2018.9   First semester

  • 実地見学(応用生命化学分野)

    2018.4 - 2018.9   First semester

  • 分子細胞生物学

    2017.4 - 2017.9   First semester

  • コアセミナー

    2012.4 - 2012.9   First semester

  • コアセミナー

    2007.4 - 2007.9   First semester

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FD Participation

  • 2018.5   Role:Participation   Title:FD

    Organizer:[Undergraduate school/graduate school/graduate faculty]

  • 2009.10   Role:Participation   Title:農学部FD

    Organizer:[Undergraduate school/graduate school/graduate faculty]

  • 2008.4   Role:Participation   Title:農学部FD

    Organizer:[Undergraduate school/graduate school/graduate faculty]

  • 2007.4   Role:Participation   Title:農学部FD

    Organizer:[Undergraduate school/graduate school/graduate faculty]

  • 2006.7   Role:Participation   Title:不明

    Organizer:[Undergraduate school/graduate school/graduate faculty]

Outline of Social Contribution and International Cooperation activities

  • なし

Travel Abroad

  • 2000.3 - 2002.2

    Staying countory name 1:United States   Staying institution name 1:Plant Gene Expression Center, U. C. Berkeley