Updated on 2025/05/23

写真a

 
YAMADA NAOTAKA
 
Organization
Faculty of Agriculture Department of Bioscience and Biotechnology Assistant Professor
School of Agriculture Department of Bioresource and Bioenvironment(Concurrent)
Graduate School of Bioresource and Bioenvironmental Sciences Department of Bioscience and Biotechnology(Concurrent)
Title
Assistant Professor
Contact information
メールアドレス
Tel
0928024720
Profile
It is conducting research on the molecular design of nobel plant growth regulators and the elucidation of the mechanism of action of some plant growth regulators. In particular, it is currently involved in elucidating the mechanisms of stomatal opening/closing and opening of plant hormones and other substances, as well as the mechanism of action of phenylalanine ammonia-lyase inhibitors in plants. In education, it is in charge of lectures on bioorganic chemistry and student experiments on organic chemistry and physical chemistry.
External link

Degree

  • Doctor of Agliculture

Research History

  • 大日本インキ化学工業株式会社 総合研究所   

    大日本インキ化学工業株式会社 総合研究所

Research Interests・Research Keywords

  • Research theme: Elucidation of the relationship between stomatal closing activity of various plant hormones and plant growth regulators and K+/H EXCHANGE ANTIPORTER 3 (KEA3) in guard cell chloroplast

    Keyword: guard cell, phytohormones, plant growth regulators, K+/H+ antiporter

    Research period: 2020.4

  • Research theme: Search for stomatal open/close inducers of gaseous signaling molecules (nitric oxide and hydrogen sulfide) in guard cells and clarify of their mechanisms of action., and research on its applied use in agriculture.

    Keyword: guard cell, phytohormones, active oxygen, nitric oxide, hydrogen sulfide

    Research period: 2018.4

  • Research theme: Functional elucidation of silkworm pesticide metabolic enzymes (gutathione transferase/sulfotransferasesbase) on protein structure information

    Keyword: silkworm, protein structure、gutathione transferase, sulfotransferasesbase,

    Research period: 2012.4 - 2023.3

Papers

  • Chloroplast K+/H+ EXCHANGE ANTIPORTER 3 modulates abscisic acid-induced reactive oxygen species generation in guard cells Reviewed International journal

    @山田 直隆, @遠城 道雄, @園池公毅, @島崎 研一郎, @岩井 純夫

    Physiologia Plantarum   3 ( 5 )   2024.1   ISSN:0031-9317 eISSN:1399-3054

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Physiologia Plantarum  

    Reactive oxygen species (ROS) are important signaling molecules in stomatal closure. In a previous report, we demonstrated that ROS generated through photosynthetic electron transport (PET) act as signaling molecules in abscisic acid (ABA)-induced stomatal closure. However, the mechanism by which ABA induces ROS generation through PET remains unclear. Here, we assessed the possibility that chloroplast K+/H+ EXCHANGE ANTIPORTER 3 (KEA3) functions in ABA-induced ROS generation in guard cells, resulting in stomatal closure. KEA3 localizes to a thylakoid membrane and allows proton efflux from the thylakoid lumen by K+/H+ antiport, regulating photosynthesis by proton motive force. KEA3 loss-of-function mutants (kea3-1 and kea3-2) were impaired in ABA-induced ROS generation of guard cells and stomatal closure. The small molecule electroneutral K+/H+ antiporter nigericin induced ROS
    generation in guard cells and stomatal closures in the kea3 mutants. This study demonstrates that KEA3 is an important factor for ABA-induced ROS generation in guard cells and stomatal closure

    DOI: 10.1111/ppl.14136

    Web of Science

    Scopus

    Other Link: https://doi.org/10.1002/pld3.137

    Repository Public URL: https://hdl.handle.net/2324/7348020

  • Characterization of glutathione-specific gamma glutamyl cyclotransferase (ChaC) in Bombyx mori Reviewed International journal

    #Wazifa Afrin, @Naotaka Yamada, @Shigeki Furuya, @Kohji Yamamoto

    Arch Insect Biochem Physiol   114 ( 1 )   e22027   2023.4   ISSN:0739-4462 eISSN:1520-6327

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Archives of Insect Biochemistry and Physiology  

    Glutathione (GSH) contributes to redox maintenance and detoxification of various xenobiotic and endogenous substances. γ-glutamyl cyclotransferase (ChaC) is involved in GSH degradation. However, the molecular mechanism underlying GSH degradation in silkworms (Bombyx mori) remains unknown. Silkworms are lepidopteran insects that are considered to be an agricultural pest model. We aimed to examine the metabolic mechanism underlying GSH degradation mediated by B. mori ChaC and successfully identified a novel ChaC gene in silkworms (herein, bmChaC). The amino acid sequence and phylogenetic tree revealed that bmChaC was closely related to mammalian ChaC2. We overexpressed recombinant bmChaC in Escherichia coli, and the purified bmChaC showed specific activity toward GSH. Additionally, we examined the degradation of GSH to 5-oxoproline and cysteinyl glycine via liquid chromatography–tandem mass spectrometry. Quantitative real-time polymerase chain reaction revealed that bmChaC mRNA expression was observed in various tissues. Our results suggest that bmChaC participates in tissue protection via GSH homeostasis. This study provides new insights into the activities of ChaC and the underlying molecular mechanisms that can aid the development of insecticides to control agricultural pests.

    DOI: 10.1002/arch.22027

    Web of Science

    Scopus

    PubMed

  • Investigation of the active site of an unclassified glutathione transferase in Bombyx mori by alanine scanning Reviewed International journal

    @Kohji Yamamoto,# Misuzu Yamaguchi and @Naotaka Yamada

    Journal of Pesticide Science   45 ( 4 )   238 - 240   2020.10

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    Language:English   Publishing type:Research paper (scientific journal)  

    Glutathione transferase (GST) is an important class of detoxification enzymes that are vital for defense against various xenobiotics and cellular oxidative stress. Previously, we had reported an unclassified glutathione transferase 2 in Bombyx mori (bmGSTu2) to be responsible for detoxifying diazinon. In this study, we aimed to identify the amino acid residues that constitute a hydrogen-bonding network important for GST activity. Site-directed mutagenesis of bmGSTu2 suggested that residues Asn102, Pro162, and Ser166 contribute to its catalytic activity

    DOI: https://doi.org/10.1584/jpestics.D20-036

    Other Link: https://doi.org/10.1002/pld3.137

  • An omega‐class glutathione S‐transferase in the brown planthopper Nilaparvata lugens exhibits glutathione transferase and dehydroascorbate reductase activities Reviewed International journal

    #Fumiko Saruta, @Naotaka Yamada, @Kohji Yamamoto

    Arch Insect Biochem. Physiol.   2019.7

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    Language:English   Publishing type:Research paper (scientific journal)  

    A complementary DNA that encodes an omega‐class glutathione S‐transferase (GST) of the brown planthopper, Nilaparvata lugens (nlGSTO), was isolated by reverse transcriptase polymerase chain reaction. A recombinant protein (nlGSTO) was obtained via overexpression in the Escherichia
    coli cells and purified. nlGSTO catalyzes the biotransformation of glutathione with 1‐chloro‐2,4‐dinitrobenzene, a general substrate for GST, as well as with dehydroascorbate to synthesize ascorbate. Mutation experiments revealed that putative substrate‐binding sites, including Phe28, Cys29, Phe30, Arg176, and Lue225, were important for glutathione transferase and dehydroascorbate reductase activities. As ascorbate is a reducing agent, nlGSTO may participate in antioxidant resistance

    DOI: 10.1002/arch.21599

  • Guard cell photosynthesis is crucial in abscisic acid-induced stomatal closure Reviewed International journal

    @岩井 純夫, 緒方 翔, @山田 直隆, @遠城 道雄, @園池公毅, @島崎 研一郎

    Plant Direct   3 ( 5 )   2019.5

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    Language:English   Publishing type:Research paper (scientific journal)  

    Reactive oxygen species (ROS) are ubiquitous signaling molecules involved in diverse physiological processes, including stomatal closure. Photosynthetic electron transport (PET) is the main source of ROS generation in plants, but whether it functions in guard cell signaling remains unclear. Here, we assessed whether PET functions in abscisic acid (ABA) signaling in guard cells. ABA-elicited ROS were localized to guard cell chloroplasts in Arabidopsis thaliana, Commelina benghalensis, and Vicia faba in the light and abolished by the PET inhibitors 3-(3, 4-dichlorophenyl)-1, 1-dimethylurea and 2, 5-dibromo-3-methyl-6-isopropyl-p-benzoquinone. These inhibitors reduced ABA-induced stomatal closure in all three species, as well as in the NADPH oxidase-lacking mutant atrboh D/F. However, an NADPH oxidase inhibitor did not fully eliminate ABA-induced ROS in the chloroplasts, and ABA-induced ROS were still observed in the guard cell chloroplasts of atrboh D/F. This study demonstrates that ROS generated through PET act as signaling molecules in ABA-induced stomatal closure and that this occurs in concert with ROS derived through NADPH oxidase.

    DOI: 10.1002/pld3.137

    Other Link: https://doi.org/10.1002/pld3.137

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Presentations

  • サリチル酸の気孔閉鎖誘導におけるシグナル伝達物質としての硫化水素

    @山田直隆1,#新北 大樹,岩井 純夫,

    日本農芸化学会2020年度大会(福岡)  2020.3 

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    Event date: 2020.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:九州大学(福岡市)   Country:Japan  

    Role of hydrogen sulfide as a signaling material in induction of stomata closure of salicylic acid.
    Naotaka Yamada, Daiki Shinboku, Sumio Iwai.
    The 2021 Meeting of the Japan Society for Bioscience, Biotechnology and Agrochemistry.

  • 担子菌酵母フェニルアラニンアンモニアリアーゼと阻害剤Z302との相互作用に関する熱力学的解析と結晶構造解析

    @中島崇1,@山田直隆,#吉村昂大,@YAO Min,@沼田倫征,@石野良純

    日本農芸化学会大会 2020  2020.3 

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    Event date: 2020.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:九州大学 伊都キャンパス   Country:Japan  

  • ABAアゴニストQuinabactinをリード化合物とした抗ABA活性物質の探索

    #高杉航平,#福崎悠史郎,@山田直隆

    日本農薬学会第45回大会  2020.3 

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    Event date: 2020.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:大阪府立大学(堺市)   Country:Japan  

    Search for anti-ABA compounds by using ABA agonist, Quinabactin as a lead compound
    Kohei Taksugi, Yushiro Fukusaki, Naotaka Yamada (Faculty of agriculture, Kyushu Univ.),
    45th Annual Meeting of the Pesticide Science Society of Japan

  • 担子菌酵母および植物由来フェニルアラニンアンモニアリアーゼの特異的阻害剤Z302を利用した生化学的解析と結晶構造解析

    @中島崇1,#吉村昂大,#冨高大暉,@山田直隆,@石野良純

    日本農芸化学会2019年度西日本・中四国支部合同大会  2019.11 

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    Event date: 2019.11

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:琉球大学(西原町)   Country:Japan  

    Biochemical analysis and crystal structure analysis using specific inhibitor Z302 of basidiomytesal yeast and plant-derived phenylalanine ammonia lyase.
    Takashi Nakashima, Takahiro Yoshimura, Daiki Tomitaka, Naotaka Yamada, Yoshizumi Ishino.
    The 2019 Joint Meeting of The West and Chushikoku Branch of the Japan Society for Bioscience, Biotechnology and Agrochemistry.

  • サリチル酸による孔辺閉鎖誘導時の孔辺細胞内H2S、NOおよびH2O2量変化

    @山田直隆1,#新北 大樹,岩井 純夫,

    日本農芸化学会2019年度西日本・中四国支部合同大会  2019.11 

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    Event date: 2019.11

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:琉球大学(西原町)   Country:Japan  

    Changes in H2S, NO and H2O2 levels in guard cells during the guard closure induction by salicylic acid,
    Naotaka Yamada, Daiki Shinboku, Sumio Iwai.
    The 2019 Joint Meeting of The West and Chushikoku Branch of the Japan Society for Bioscience, Biotechnology and Agrochemistry.

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Professional Memberships

  • 日本農芸化学会

  • 植物化学調節学会

  • 日本農薬学会

Committee Memberships

  • 日本農薬学会   農薬学会編集委員   Domestic

    2021.4 - 2025.3   

  • 日本農薬学会   農薬学会編集委員   Domestic

    2017.4 - 2021.3   

  • 日本農芸化学会   西日本支部幹事   Domestic

    2009.4 - 2011.3   

  • 日本農芸化学会   西日本支部幹事   Domestic

    2007.4 - 2009.3   

  • 日本農薬学会   農薬バイオサイエンス研究会委員   Domestic

    2007.4 - 2009.3   

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Academic Activities

  • 座長(Chairmanship)

    日本農芸化学会2020年度福岡大会  ( Japan ) 2020.3

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    Type:Competition, symposium, etc. 

  • 福岡大会実行委員

    日本農芸化学会2020年度福岡大会  ( Japan ) 2020.3

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    Type:Competition, symposium, etc. 

  • Screening of academic papers

    Role(s): Peer review

    2020

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    Type:Peer review 

    Number of peer-reviewed articles in foreign language journals:1

  • 座長(Chairmanship)

    日本農薬学会第45回大会  ( Japan ) 2019.3

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    Type:Competition, symposium, etc. 

  • 座長(Chairmanship)

    日本農芸化学会2018年度西日本支部大会  ( Japan ) 2018.9

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    Type:Competition, symposium, etc. 

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Research Projects

  • 文部科学省・日本学術振興会科学研究費「基盤研究B」 窒素固定菌の新規な炭素源獲得戦略としての異種微生物間共生型窒素固定の解明

    2021.4 - 2025.3

    鹿児島大学 

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    Authorship:Coinvestigator(s) 

    Nitrogen, along with phosphorus and potassium, is an important and indispensable fertilizer component in plant production, and in order to use nitrogen as fertilizer, it is necessary to synthesize ammonia by immobilizing nitrogen (N2) in the atmosphere. The Haber-Bosch process, developed 100 years ago, is still the only industrial synthesis method for producing ammonia, using large amounts of oil and natural gas. In this research project, we will focus on biological nitrogen fixation methods using nitrogen-fixing bacteria, especially rhizobium bacteria that symbiotically fix nitrogen with leguminous plants, and will develop a new nitrogen-fixing method that uses rhizobium bacteria to fix nitrogen, and attempt to develop biological nitrogen fixation. Representative Sakai et al. have already found a population (consortium) composed of several microbial species capable of fixing nitrogen using cellulose isolated from cut grass sediments as a carbon (energy) source. In this study, we will clarify the ecology of each of these hetero-microbial symbiotic nitrogen-fixing bacteria, and metabolome analysis of metabolites required for symbiosis, and we will make it clear the structure, function, and ecology of nitrogen-fixing bacteria with a previously unknown ecotype, and to develop a stabilized biological nitrogen fixation method. The results of this research will provide new knowledge on nitrogen supply systems in soil ecosystems and contribute to nitrogen supply technology for soil utilizing unutilized plant biomass (cellulose) and endogenous plant nitrogen-fixing bacteria.

  • 窒素固定菌の新規な炭素源獲得戦略としての異種微生物間共生型窒素固定の解明

    Grant number:23K21170  2021.4 - 2025.3

    Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (B)

    境 雅夫, 山田 直隆, 池永 誠

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    Grant type:Scientific research funding

    本研究では「窒素固定菌の新規な炭素源獲得戦略としての異種微生物間共生型窒素固定システムの解明と利用」に関する研究を目的とする。これまで、土壌生態系における窒素固定菌は、大きく2つの生態型が推定されてきた。①土壌中で自由生活をする単生型の窒素固定菌、②植物との共生生活(緩やかな共生を含む)をする共生型の窒素固定菌、の2つの生態型である。この従来の生態型に加えて、窒素固定菌の炭素源獲得戦略の違いに基づき、新たに③セルロースを分解する嫌気性細菌と共生的に生活する「異種微生物間共生型の窒素固定菌」による新規な窒素固定システムの存在を明らかにし、その構造・機能・生態を解明する。

    CiNii Research

  • 窒素固定菌の新規な炭素源獲得戦略としての異種微生物間共生型窒素固定の解明

    Grant number:21H02108  2021 - 2024

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (B)

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    Authorship:Coinvestigator(s)  Grant type:Scientific research funding

  • 文部科学省・日本学術振興会科学研究費「挑戦的研究(萌芽)」 X線結晶構造解析による新規農薬デザイン

    2017.4 - 2020.3

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    Authorship:Principal investigator 

    最近、研究代表者によって鱗翅目昆虫のモデルであるカイコより発見された農薬・有機リン剤(ダイアジノン)を殺虫活性のないグルタチオン抱合体へ代謝する酵素(Glutathione S-Transferase Unclassified 2: GSTU2)が、コナガやハスモンヨトウ等の鱗翅目害虫に特異的に存在することを解明しており、本酵素の立体構造や発現機構など未だ解明されていない分子レベルでの特徴を明らかにすることを目的としている。そのため、X線結晶構造を基礎とした本酵素阻害剤の創製、並びに農薬耐性ならびに感受性昆虫間における本酵素発現量ならびにダイアジノン代謝物質量の差異解析、を計画している。X線結晶構造解析による新規な農薬を創製するための研究基盤を確立する。

  • サリチル酸気孔閉鎖作用における硫化水素のシグナル伝達物質としての機能解明

    Grant number:17K07698  2017 - 2019

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

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    Authorship:Principal investigator  Grant type:Scientific research funding

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Educational Activities

  • In the undergraduate School of Agriculture, it is in charge of Pesticide Chemistry I , Pesticide Chemistry II, Bioanalysis/Instrumental Analysis Methods II, Physical Chemistry Experiment, Agricultural Chemistry Experiment(Part of Organic Analysis and Organic Synthesis )in the Course of Agricultural Chemistry.
    In the graduate School of Agriculture, it is in charge of Molecular Biosciences, Molecular Biosciences Project Exercises in the Course of Molecular Biosciences.

Class subject

  • 農薬化学Ⅱ

    2023.12 - 2024.2   Winter quarter

  • 生物機能分子化学演習第一

    2023.10 - 2024.3   Second semester

  • 物理化学実験

    2023.10 - 2024.3   Second semester

  • 農薬化学Ⅰ

    2023.10 - 2023.12   Fall quarter

  • 生物解析・機器分析法II

    2023.6 - 2023.8   Summer quarter

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FD Participation

  • 2024.3   Role:Participation   Title:有体物管理センターの業務および成果有体物収入の配分率の変更について

    Organizer:University-wide

  • 2023.11   Role:Participation   Title:農学研究院FD:遺伝子組換え実験の安全管理について

    Organizer:[Undergraduate school/graduate school/graduate faculty]

  • 2023.9   Role:Participation   Title:M2Bシステムの使い方 ~Moodleのバージョンアップによる変更点を中心に紹介します~

    Organizer:University-wide

  • 2022.3   Role:Participation   Title:農学研究院FD:国費特別プログラム「未来の農を描くビジョンデザイン実践プログラム」実施に向けて

    Organizer:[Undergraduate school/graduate school/graduate faculty]

  • 2021.11   Role:Participation   Title:農学研究院FD「人を対象とする生命科学・医学系研究に関する倫理指針」について

    Organizer:[Undergraduate school/graduate school/graduate faculty]

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Visiting, concurrent, or part-time lecturers at other universities, institutions, etc.

  • 2023  東海大学・大学院農学研究科  Classification:Part-time lecturer  Domestic/International Classification:Japan 

    Semester, Day Time or Duration:集中(3月4日~7日)

  • 2023  福岡農業高校 専攻科  Classification:Part-time lecturer  Domestic/International Classification:Japan 

    Semester, Day Time or Duration:前期・後期  月曜日1時限

  • 2022  福岡農業高校 専攻科  Classification:Part-time lecturer  Domestic/International Classification:Japan 

    Semester, Day Time or Duration:前期・後期  月曜日1時限

  • 2022  東海大学・大学院農学研究科  Classification:Part-time lecturer  Domestic/International Classification:Japan 

    Semester, Day Time or Duration:集中(3月6日~9日)

  • 2021  東海大学・大学院農学研究科  Classification:Part-time lecturer  Domestic/International Classification:Japan 

    Semester, Day Time or Duration:集中(3月7日~10日)

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Social Activities

  • 卒業研究 「廃竹に含まれるフェニルプロパノイド物質の測定」における実験指導

    福岡農業高校 専攻科 食品工学科 微生物工学科  2012.12

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    Audience:Infants, Schoolchildren, Junior students, High school students

    Type:Seminar, workshop

  • 卒業研究「Lactobacillus sp. FJAS 201 の農薬減少高価」における実験指導

    福岡農業高校 専攻科 食品工学科 微生物工学科  2007.11

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    Audience:Infants, Schoolchildren, Junior students, High school students

    Type:Seminar, workshop

  • 卒業研究「Lactobacillus の農薬分解」における実験指導

    福岡農業高校 専攻科 食品工学科 微生物工学科  2006.12

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    Audience:Infants, Schoolchildren, Junior students, High school students

    Type:Other

Travel Abroad

  • 2003.10 - 2004.9

    Staying countory name 1:Germany   Staying institution name 1:Johann Wolfgang Goethe-Universitat