Updated on 2024/10/29

写真a

 
MATSUMOTO SHUNSUKE
 
Organization
Faculty of Agriculture Department of Bioscience and Biotechnology Assistant Professor
School of Agriculture Department of Bioresource and Bioenvironment(Concurrent)
Graduate School of Bioresource and Bioenvironmental Sciences Department of Bioscience and Biotechnology(Concurrent)
Title
Assistant Professor
Contact information
メールアドレス
Tel
0928024717
Profile
CRISPR-Casシステムは、原核生物が持つ獲得免疫機構であり、現在それを応用したゲノム編集技術が急速に開発されています。私は当研究室で独自に見出したCRISPR-Casシステムについて、生化学、構造生物学、細胞生物学などさまざまな手法を駆使して、(1)Casタンパク質の作動機構の原子分解能レベルでの解明、(2)Casタンパク質の機能-構造情報を基にデザインした改変体の創出、(3)様々な生物に適応できるゲノム編集技術の開発そして(4)迅速かつ高感度な核酸検出法への応用を目指しています.  真核生物の細胞には、生体膜で仕切られた様々な細胞小器官(オルガネラ)が高度に発達し、さまざまな生物現象を支えています。オルガネラが正常に機能するためには、合成されたタンパク質が正確に各オルガネラに配送される必要があります。近年私たちは、タンパク質のオルガネラ局在化における配送やり直しの仕組みを見出し、その分子機構を出芽酵母を用いて解析しています。
External link

Research Areas

  • Life Science / Cell biology

  • Life Science / Functional biochemistry

  • Life Science / Structural biochemistry

Degree

  • Systems Life Sciences

Research History

  • Kyushu University Faculty of Agriculture tenure-track assistant professor 

    2020.8 - Present

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  • 就職実績有, 京都産業大学 2014年4月〜2020年7月   

Research Interests・Research Keywords

  • Research theme: タンパク質輸送

    Keyword: タンパク質輸送

    Research period: 2024

  • Research theme: オルガネラ

    Keyword: オルガネラ

    Research period: 2024

  • Research theme: 構造生物学

    Keyword: 構造生物学

    Research period: 2024

  • Research theme: ミトコンドリア

    Keyword: ミトコンドリア

    Research period: 2024

  • Research theme: Proofreading mechanism of protein mislocalition

    Keyword: Protein sorting, proofreading of protein mislocalization, mitochondria, Msp1

    Research period: 2022.6

  • Research theme: Structural biology of novel CRISPR-Cas related proteins

    Keyword: CRISPR, CRISPR-associated proteins, Structural biology

    Research period: 2020.8 - 2022.3

Awards

  • 2022年度 JAICI賞

    2022.12   一般社団法人化学情報協会  

  • 2022年度 日本生化学会奨励賞

    2022.11   日本生化学会  

  • 2022年度日本生化学会奨励賞

    2022.11   日本生化学会   膜タンパク質局在化における配送校正機構の解明

    松本 俊介

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  • JAICI賞

    2022   一般社団法人化学情報協会  

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Papers

  • GET pathway mediates transfer of mislocalized tail-anchored proteins from mitochondria to the ER Reviewed International journal

    Matsumoto S., Ono S., Shinoda S., Kakuta C., Okada S., Ito T., Numata T., Endo T.

    Journal of Cell Biology   221 ( 221 )   e202104076   2022.6   ISSN:0021-9525 eISSN:1540-8140

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    Language:Japanese   Publishing type:Research paper (scientific journal)   Publisher:Journal of Cell Biology  

    Tail-anchored (TA) membrane proteins have a potential risk to be mistargeted to the mitochondrial outer membrane (OM). Such mislocalized TA proteins can be extracted by the mitochondrial AAA-ATPase Msp1 from the OM and transferred to the ER for ER protein quality control involving ubiquitination by the ER-resident Doa10 complex. Yet it remains unclear how the extracted TA proteins can move to the ER crossing the aqueous cytosol and whether this transfer to the ER is essential for the clearance of mislocalized TA proteins. Here we show by time-lapse microscopy that mislocalized TA proteins, including an authentic ER-TA protein, indeed move from mitochondria to the ER in a manner strictly dependent on Msp1 expression. The Msp1-dependent mitochondria-to-ER transfer of TA proteins is blocked by defects in the GET system, and this block is not due to impaired Doa10 functions. Thus, the GET pathway facilitates the transfer of mislocalized TA proteins from mitochondria to the ER.

    DOI: 10.1083/jcb.202104076

    Web of Science

    Scopus

    PubMed

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  • Msp1 Clears Mistargeted Proteins by Facilitating Their Transfer from Mitochondria to the ER. Reviewed International journal

    Matsumoto, S., Nakatsukasa, K., Kakuta, C., Tamura, Y., Esaki, M., Endo, T.

    Molecular Cell   76 ( 1 )   191 - 205   2019.8

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    Language:Japanese   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.molcel.2019.07.006

  • Tethering an N-Glycosylation Sequon-Containing Peptide Creates a Catalytically Competent Oligosaccharyltransferase Complex. Reviewed International journal

    Matsumoto, S., Taguchi, Y., Shimada, A., Igura, M., Kohda, D.

    Biochemistry   56 ( 4 )   602 - 611   2017.1

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    Language:Japanese   Publishing type:Research paper (scientific journal)  

    DOI: 10.1021/acs.biochem.6b01089

  • Crystal structures of an archaeal oligosaccharyltransferase provide insights into the catalytic cycle of N-linked protein glycosylation. Reviewed International journal

    Matsumoto, S., Shimada, A., Nyirenda, J., Igura, M., Kawano, Y., Kohda, D.

    Proc. Natl. Acad. Sci. U S A.   110 ( 44 )   17868 - 17873   2013.10

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    Language:Japanese   Publishing type:Research paper (scientific journal)  

    DOI: 10.1073/pnas.1309777110

  • オーキシンデグロン法を用いた出芽酵母におけるペルオキシソーム膜タンパク質の局在解析

    小暮 佳希, 小野 鈴花, 岡田 悟, 沼田 倫征, 遠藤 斗志也, 松本 俊介

    日本生化学会大会プログラム・講演要旨集   96回   [1P - 273]   2023.10

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    Language:Japanese   Publisher:(公社)日本生化学会  

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Books

  • Analysis of protein trafficking between mitochondria and the endoplasmic reticulum by fluorescence microscopy

    Matsumoto S., Ono S., Endo T.

    Methods in Enzymology  2024    ISSN:00766879

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    Precise protein localization is essential for normal cellular functions. However, recent studies have revealed that protein targeting is error-prone, and tail-anchored proteins mistargeted to mitochondria are transferred to the endoplasmic reticulum (ER) by an ATPase Msp1 (yeast)/ATAD1 (human) in the mitochondrial outer membrane for further quality examination in the ER to determine their fate, degradation or re-targeting. Analysis of the inter-organelle transfer of proteins requires a combination of time-lapse fluorescence microscopy and a system to achieve regulation of the protein levels of both transfer substrates and factors regulating the transfer in a coordinated manner at precise timing. This can be achieved by using a promoter switch for expression and acute depletion of involved factors through the degron-based proteasome system. In this chapter, we will describe methods to analyze inter-organelle protein transfer by fluorescence microscope within living yeast cells, by using the example of Msp1-mediated transfer of mistargeted proteins from mitochondria to the ER.

    DOI: 10.1016/bs.mie.2024.07.041

    Scopus

Presentations

  • 膜タンパク質局在化における配送校正機構の解明 Invited

    松本 俊介、小野 鈴花、遠藤 斗志也

    第95回日本生化学会大会  2022.11 

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    Event date: 2022.11

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Country:Japan  

  • ミトコンドリア外膜に誤配送されたテイルアンカー型タンパク質の配送校正機構 Invited

    松本俊介、小野鈴花、遠藤斗志也

    第22回日本蛋白質科学会年会  2022.6 

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    Event date: 2022.6

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:つくば   Country:Japan  

  • Proofreading of protein mislocalization mediated by a mitochondrial AAA-ATPase Msp1 Invited

    Shunsuke Matsumoto, Suzuka Ono, Toshiya Endo

    第44回日本分子生物学会年会  2021.1

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    Event date: 2021.12

    Language:English   Presentation type:Symposium, workshop panel (public)  

    Venue:横浜   Country:Japan  

  • テイルアンカー型タンパク質の局在化における配送校正機構の解析

    松本 俊介

    令和4年度日本生化学会 九州支部例会  2022.6 

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    Event date: 2022.6 - 2023.6

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  • ミトコンドリアAAA-ATPアーゼMsp1による誤配送タンパク質の配送校正機構 Invited

    松本俊介、小野鈴花、遠藤斗志也

    第94回日本生化学会大会  2021.11 

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    Event date: 2021.11 - 2022.11

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:オンライン   Country:Japan  

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MISC

  • Msp1-mediated proofreading mechanism for localization of tail-anchored membrane proteins Reviewed

    Shunsuke Matsumoto

    Journal of Biochemistry   2023.3

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    Language:Japanese   Publishing type:Article, review, commentary, editorial, etc. (scientific journal)  

    DOI: 10.1093/jb/mvad025.

  • Proofreading of protein localization mediated by a mitochondrial AAA-ATPase Msp1 Reviewed International journal

    Shunsuke Matsumoto, Toshiya Endo

    Journal of Biochemistry   173 ( 4 )   265 - 271   2023.3

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    Language:Japanese   Publishing type:Article, review, commentary, editorial, etc. (scientific journal)   Publisher:(公社)日本生化学会  

    Normal cellular functions rely on correct protein localization within cells. Protein targeting had been thought to be a precise process, and even if it fails, the mistargeted proteins were supposed to be quickly degraded. However, this view is rapidly changing. Tail-anchored (TA) proteins are a class of membrane proteins that possess a single transmembrane domain (TMD) near the C-terminus and are posttranslationally targeted to the endoplasmic reticulum (ER) membrane, mitochondrial outer membrane (OM), and peroxisomal membrane, yet they can be mistargeted to the mitochondrial OM. The mistargeted TA proteins can be extracted from the OM by a mitochondrial AAA-ATPase Msp1/ATAD1 and transferred to the ER. If they are regarded as aberrant by the ER protein quality control system, they are extracted from the ER membrane for proteasomal degradation in the cytosol. If they are not regarded as aberrant, they are further transported to downstream organelles or original destinations along the secretory pathway. Thus, Msp1 contributes to not only degradation but also "proofreading" of the targeting of mislocalized TA proteins.

    DOI: 10.1093/jb/mvac097

    PubMed

    J-GLOBAL

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  • テイルアンカー型タンパク質のオルガネラ局在化における配送校正機構 Reviewed

    松本 俊介

    Journal of Japanese Biochemical Society   2023.2

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    Language:Japanese   Publishing type:Article, review, commentary, editorial, etc. (scientific journal)  

    DOI: 10.14952/SEIKAGAKU.2023.950017

  • 膜タンパク質の局在化における配送校正機構 Reviewed

    松本 俊介

    化学と生物   2022.10

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    Language:Japanese   Publishing type:Article, review, commentary, editorial, etc. (scientific journal)  

  • ミトコンドリアAAA-ATPアーゼMsp1による誤配送タンパク質の配送校正機構 Reviewed

    松本俊介, 遠藤斗志也

    生化学   2022.6

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    Language:Japanese   Publishing type:Article, review, commentary, editorial, etc. (scientific journal)  

    DOI: 10.14952/SEIKAGAKU.2021.930512

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Professional Memberships

  • 日本蛋白質科学会、日本細胞生物学会、日本生化学会、日本農芸化学会

  • 日本農芸化学会

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  • 日本蛋白質科学会

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  • 日本生化学会

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Research Projects

  • タンパク質局在化における校正機構の分子構造基盤の解明

    Grant number:23K27130  2023.4 - 2026.3

    Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (B)

    松本 俊介

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    Grant type:Scientific research funding

    申請者らは、細胞にはタンパク質の配送をやり直す仕組み、すなわちタンパク質の局在化を校正するシステムが存在することを見出し、その分子機構を明らかにしてきた。本研究は、出芽酵母をモデル系とし、小胞体膜タンパク質、ペルオキシソーム膜タンパク質そしてミトコンドリア前駆体タンパク質について、配送やり直しの検証、分子機構の解明を目指す。そして、クライオ電子顕微鏡解析による膜引き抜きATPアーゼMsp1と基質複合体の立体構造を決定し、Msp1による基質認識および膜引き抜き機構の解明を目指す。

    CiNii Research

  • 独自のメタゲノムデータに基づく新規CRISRP-Cas系の機能構造解析と探索

    Grant number:23K17995  2023 - 2025

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Challenging Research(Exploratory)

    松本 俊介

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    Authorship:Principal investigator  Grant type:Scientific research funding

    当研究室では、環境サンプルのメタゲノム解析を独自に行い、新規CRISPR-Cas系エフェクターを探索してきた。我々はこれまでに各地温泉水を採取して調製したDNAのメタゲノム解析からユニークな配列を持つCas9とCas12aが、日本沿岸各地の海水を採取して調製したDNAのメタゲノム解析から小型のCasタンパク質が複数見出した。本研究はこれら新規Casエフェクター候補の機能と構造を解析すると共に、新たにメタゲノム解析を行い、機能未知なCas候補を独自に探索し、ゲノム編集ツールの幅を広げる新たな技術開発を目指す。

    CiNii Research

  • タンパク質局在化における校正機構の分子構造基盤の解明

    Grant number:23H02437  2023 - 2025

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (B)

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    Authorship:Principal investigator  Grant type:Scientific research funding

  • 新規CRISPR-Casシステムを利用したゲノム編集ツール開発

    2022 - 2023

    令和4年度大学院農学研究院若手教員支援事業(共同研究プロジェクト支援)

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    Authorship:Principal investigator  Grant type:On-campus funds, funds, etc.

  • 木下基礎科学研究基金助成/タンパク質の誤配送を校正する膜引き抜き酵素の構造学的研究

    2022

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    Grant type:Donation

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Class subject

  • Microbiology

    2024.4 - 2024.9   First semester

  • 生物機能分子化学Ⅰ(E科目)

    2024.4 - 2024.6   Spring quarter

  • Microbiology Ⅱ

    2023.6 - 2023.8   Summer quarter

  • 生物機能分子化学Ⅱ

    2023.4 - 2023.9   First semester

  • 生物機能分子化学Ⅰ

    2022.4 - 2022.9   First semester