KOBAYASHI YOSHIAKI
Organization
Medical Institute of Bioregulation Medical Research Center for High Depth Omics Assistant Professor
Title
Assistant Professor
Contact information
External link
ORCID
Research Areas
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Life Science / Molecular biology
Degree
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博士（理学） ( 2021.3 東京大学 )
Research History
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Kyushu University Division of Gene Expression Dynamics, Medical Research Center Initiative for High Depth Omics, Medical Institute of Bioregulation Assistant professor (special project faculty)
2024.4 - Present
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The University of Tokyo Graduate School of Science Department of Biological Sciences Research Fellow
2021.4 - 2024.3
Country：Japan
Education
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The University of Tokyo
2018.4 - 2021.3
Country： Japan
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The University of Tokyo
2016.4 - 2018.3
Country： Japan
Research Interests・Research Keywords
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Research theme：Spatial Omics
Keyword：sequential FISH (seq-FISH)
Research period： 2024.4 - Present
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Research theme：Nucleic asid therapeutics
Keyword：siRNA
Research period： 2016.4 - 2024.3
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Research theme：Nucleic asid therapeutics
Keyword：microRNA
Research period： 2016.4 - 2024.3
Papers
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Kohei Nomura, Seongjin An, Yoshiaki Kobayashi, Jiro Kondo, Ting Shi, Hirotaka Murase, Kosuke Nakamoto, Yasuaki Kimura, Naoko Abe, Kumiko Ui-Tei, Hiroshi Abe
Nucleic Acids Research 2024.9 （ ISSN:0305-1048 eISSN:1362-4962 ）
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Shohei Maruyama, Takashi Kusakabe, Xinyi Zou, Yoshiaki Kobayashi, Yoshimasa Asano, Qingbo S. Wang, Kumiko Ui-Tei
Methods in Molecular Biology 49 - 62 2023 （ ISSN:1064-3745 ISBN:9781071630150 eISSN:1940-6029 ）
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A 2'-modified uridine analog, 2'-O-(methylthiomethoxy)methyl uridine, for siRNA applications Reviewed International journal
Lyu Fangjie, Seongjin An, Yoshiaki Kobayashi, Kohei Nomura, Rintaro Baba, Naoko Abe, Haruka Hiraoka, Fumitaka Hashiya, Zhaoma Shu, Kumiko Ui-Tei, Yasuaki Kimura, Hiroshi Abe
Bioorganic & Medicinal Chemistry Letters 74 128939 - 128939 2022.10 （ ISSN:0960-894X ）
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Knockdown of 15-bp Deletion-Type v-raf Murine Sarcoma Viral Oncogene Homolog B1 mRNA in Pancreatic Ductal Adenocarcinoma Cells Repressed Cell Growth In Vitro and Tumor Volume In Vivo Invited Reviewed International journal
Jiaxuan Song*, Yoshiaki Kobayashi* (*co-first author), Yoshimasa Asano, Atsushi Sato, Hiroaki Taniguchi, Kumiko Ui-Tei
Cancers 14 ( 13 ) 3162 - 3162 2022.6 （ eISSN:2072-6694 ）
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Kobayashi Y, Tian S, Ui-Tei K
Research Aspects in Biological Science 2 117 - 130 2022.5
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Yoshiaki Kobayashi, Shen Tian, Kumiko Ui-Tei
Genes 13 ( 2 ) 319 - 319 2022.2 （ eISSN:2073-4425 ）
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Yoshiaki Kobayashi, Daiki Fukuhara, Dai Akase, Misako Aida, Kumiko Ui-Tei
ACS Omega 7 ( 2 ) 2398 - 2410 2022.1 （ ISSN:2470-1343 eISSN:2470-1343 ）
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Selection of Chemical Modifications in the siRNA Seed Region That Repress Off-Target Effect
Yoshiaki Kobayashi;Kengo Miyamoto;Misako Aida;Kumiko Ui-Tei
Methods in Molecular Biology 17 - 30 2021.4
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A robust model for quantitative prediction of the silencing efficacy of wild-type and A-to-I edited miRNAs Reviewed
Shen Tian, Goro Terai, Yoshiaki Kobayashi, Yasuaki Kimura, Hiroshi Abe, Kiyoshi Asai, Kumiko Ui-Tei
17 ( 2 ) 264 - 280 2019.11
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Chemical Modification of the siRNA Seed Region Suppresses Off-Target Effects by Steric Hindrance to Base-Pairing with Targets Reviewed
Hanna Iribe;Kengo Miyamoto;Tomoko Takahashi;Yoshiaki Kobayashi;Jastina Leo;Misako Aida;Kumiko Ui-Tei
ACS Omega 2 ( 5 ) 2055 - 2064 2017.5
Books
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核酸医薬 : モダリティ・合成・分析・DDSの最新動向
浅野吉政, 小林芳明, 程久美子（ Role： Contributor第1章モダリテイ 第２節siRNAとmicroRNA）
エヌ・ティー・エス 2024.4 （ ISBN:9784860438869 ）
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核酸医薬・mRNA医薬の製造分析の基礎と基盤技術開発
小林芳明, 程久美子（ Role： Contributorオフターゲット効果を回避する siRNA 医薬の分子設計）
シーエムシー・リサーチ 2023.2 （ ISBN:9784910581347 ）
Presentations
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Development of SNPD-siKRAS which specifically represses KRAS gene with single nucleotide mutation.
Yoshiaki Kobayashi, Yoshimasa Asano, Hiroaki Taniguchi, Kumiko Ui-Tei
2023.7
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Specific knockdown of KRAS mutant gene using SNPD-siRNA repressed cell proliferation of pancreatic cancer cells in vitro and in vivo
Yoshiaki Kobayashi, Yoshimasa Asano, Atsushi Sato, Hiroaki Taniguchi, Kumiko Ui-Tei
2022.10
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The siRNA Seed Region Is Composed of Two Functionally Different Domains Which Have Different Effects On RNA Interference and Off-target Activities in Response to 2´-OMe Modifications. International conference
Yoshiaki Kobayashi, Daiki Fukuhara, Dai Akase, Misako Aida, Kumiko Ui-Tei
The Fourth RAS Initiative Symposium 2022.10
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Development of single nucleotide polymorphism-distinguishable siRNA (SNPD-siRNA) which specifically repress the disease-associated genes with single nucleotide mutations. Invited
2022.7
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The siRNA seed region is divided into two functionally different domains in RNA interference in response to 2´-OMe modifications.
Yoshiaki Kobayashi, Daiki Fukuhara, Dai Akase, Misako Aida, Kumiko Ui-Tei
2022.7
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Development and characterization of SNPD-siKRAS which specifically represses KRAS gene with single nucleotide mutation.
Yoshiaki Kobayashi, Yoshimasa Asano, Hiroaki Taniguchi, Kumiko Ui-Tei
2023.12
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Development and verification of a single nucleotide mutation-distinguishable siRNA for PIK3CA (SNPD-siPIK3CA).
Toshinori Ohyama, Yoshiaki Kobayashi, Susumu Goyama, Kumiko Ui-Tei
2023.7
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The 2'-formamide modification in the siRNA seed region avoids off-target effects by steric hindrance.
Seongjin An, Yoshiaki Kobayashi, Kohei Nomura, Yasuaki Kimura, Hiroshi Abe, Akase Dai, Misako Aida, Kumiko Ui-Tei
2023.7
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Development of single nucleotide mutation-distinguishable siRNA for PIK3CA (SNPD-siPIK3CA) and its effects on cancer cells. International conference
Toshinori Ohyama, Yoshiaki Kobayashi, Susumu Goyama, Kumiko Ui-Tei
CSHL Asia, The Now and Future of RNA Therapeutics 2023.6
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Reducing Off-Target Effects of siRNA by Introducing 2'-Formamide Modification in the Seed Region. International conference
Seongjin An, Yoshiaki Kobayashi, Kohei Nomura, Yasuaki Kimura, Hiroshi Abe, Akase Dai, Misako Aida, Kumiko Ui-Tei
2023.6
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A new technology of siRNA that specifically represses the growth of human pancreatic cancer by reducing the mutated KRAS expression. International conference
Kumiko Ui-Tei, Yoshiaki Kobayashi, Yoshimasa Asano, Hiroaki Taniguchi
2023.5
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Prediction of Structural Change of siRNA by 2'-formamide, a Newly-synthesized Chemical Modification, via Density Functional Theory.
Seongjin An, Yoshiaki Kobayashi, Kohei Nomura, Yasuaki Kimura, Hiroshi Abe, Dai Akase, Misako Aida, Kumiko Ui-Tei
2022.10
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Development of the CRISPR system to regulate TERT expression utilizing single nucleotide mutations in the promoter region.
Hiromu Matsui, Yoshiaki Kobayashi, Kumiko Ui-Tei
2022.12
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Verification of SNPD-siPIK3CA targeting single nucleotide mutation in PIK3CA oncogene in breast cancer-derived cells.
Toshinori Ohyama, Yoshiaki Kobayashi, Kumiko Ui-Tei
2022.12
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Development of siRNA which specifically represses the expression of oncogenic fusion gene.
Soto Okubo, Yoshiaki Kobayashi, Kumiko Ui-Tei
2022.12
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Thermodynamic and Structural Impacts of Chemical Modifications in the siRNA Seed Region on Off-Target Effects.
Seongjin An, Yoshiaki Kobayashi, Kohei Nomura, Yasuaki Kimura, Hiroshi Abe, Akase Dai, Misako Aida, Kumiko Ui-Tei
2024.6
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The promising chemical modifications in the siRNA seed region that can avoid off-target effects by steric hindrance or thermodynamic stability.
Seongjin An, Yoshiaki Kobayashi, Kohei Nomura, Yasuaki Kimura, Hiroshi Abe, Akase Dai, Misako Aida, Kumiko Ui-Tei
2023.12
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Thermodynamic and Structural Analysis of the Effects of Chemical Modifications in the siRNA Seed Region on Off-Target Effects.
Seongjin An, Yoshiaki Kobayashi, Kohei Nomura, Yasuaki Kimura, Hiroshi Abe, Akase Dai, Misako Aida, Kumiko Ui-Tei
2024.7
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siRNAs discriminate single nucleotide differences in PIK3CA.
Toshinori Ohyama, Yoshiaki Kobayashi, Yoshimasa Asano, Susumu Goyama, Kumiko Ui-Tei
2024.6
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Verification of SNPD-siRNA targeting single nucleotide mutation in PIK3CA oncogene
2022.8
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Development and validation of a single nucleotide mutation-distinguishable siRNA for PIK3CA (SNPD-siPIK3CA).
Toshinori Ohyama, Yoshiaki Kobayashi, Yoshimasa Asano, Susumu Goyama, Kumiko Ui-Tei
2024.7
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Identification of kinase for TNRC6A that regulates miRNA-mediated RNA silencing.
Shen Li, Masataka Suzawa, Efe Begar, Yoshiaki Kobayashi, Kumiko Ui-Tei
2023.6
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Development of siRNA specific for PIK3CA proto-oncogene with single-nucleotide mutation and its effect on cancer cell proliferation.
Toshinori Ohyama, Yoshiaki Kobayashi, Yoshimasa Asano, Susumu Goyama, Kumiko Ui-Tei
2023.12
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Development of a CRISPR-Cas9 system that targets only harmful bacteria or metabolites.
Ryuichi Ono, Yoshiaki Kobayashi, Kumiko Ui-Tei
2023.12
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Development of a CRISPR system that distinguishes single nucleotide mutations in the TERT promoter to suppress carcinogenesis.
Taku Yunomae, Hiromu Matsui, Yoshiaki Kobayashi, Kumiko Ui-Tei
2023.12
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Verification of CRISPR-Cas9 system for sequence-specific regulation of the gut microbiota.
Ryuichi Ono, Yoshiaki Kobayashi, Kumiko Ui-Tei
2022.12
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Cell-derived Xenograftモデルを用いたKRASやBRAFの変異部位を対象としたsiRNAの有効性の検証
浅野吉政, 小林芳明, 佐藤淳, 谷口博昭, 程久美子
2022.8
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2’位化学修飾核酸の導入による siRNA の標的特異性の向上
野村 浩平, 成鎮, 村瀬 裕貴, 中本 航介, 木村 康明, 阿部奈保子, 小林芳明, 程 久美子, 近藤 次郎, 阿部 洋
第 9 回日本核酸医薬学会 若手シンポジウム 2024.7
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The 2’-formamide modification in the siRNA seed region reduces off-target effects by distorting the direction of the bases.
2022.7
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The 2’-formamide modification in the siRNA seed region reduces off-target effects by two different mechanisms.
Seongjin An, Yoshiaki Kobayashi, Kohei Nomura, Yasuaki Kimura, Hiroshi Abe, Dai Akase, Misako Aida, Kumiko Ui-Tei
2022.12
MISC
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siRNAが働く仕組みと医薬品としての研究開発の現状—Mechanisms of RNA interference and the current status of research and development of siRNA drugs
小林 芳明, 浅野 吉政, 程 久美子
循環器内科 = Cardioangiology / 循環器内科編集委員会 編 93 ( 3 ) 346 - 354 2023.3 （ ISSN:1884-2909 ）
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Development of siRNA specific for PIK3CA proto-oncogene with single-nucleotide mutation and its effect on cancer cell proliferation
大山隼礼, 小林芳明, 浅野吉政, 合山進, 程久美子, 程久美子
日本分子生物学会年会プログラム・要旨集(Web) 46th 2023
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Development of a CRISPR-Cas9 system that targets only harmful bacteria or metabolites in human gut microbiota
小野隆一, 小林芳明, 程久美子, 程久美子
日本分子生物学会年会プログラム・要旨集(Web) 46th 2023
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Development of a CRISPR system that distinguishes single nucleotide mutations in the TERT promoter to suppress carcinogenesis.
湯之前拓, 松井宏睦, 小林芳明, 程久美子, 程久美子
日本分子生物学会年会プログラム・要旨集(Web) 46th 2023
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Development and characterization of SNPD-siKRAS which specifically represses KRAS gene with single nucleotide mutation.
小林芳明, 浅野吉政, 谷口博昭, 程久美子
日本分子生物学会年会プログラム・要旨集(Web) 46th 2023
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Development of the CRISPR system to regulate TERT expression utilizing single nucleotide mutations in the promoter region
松井宏睦, 小林芳明, UI-TEI Kumiko, UI-TEI Kumiko
日本分子生物学会年会プログラム・要旨集(Web) 45th 2022
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Development of siRNA which specifically represses the expression of oncogenic fusion gene
大久保壮途, 小林芳明, UI-TEI Kumiko, UI-TEI Kumiko
日本分子生物学会年会プログラム・要旨集(Web) 45th 2022
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Verification of SNPD-siPIK3CA targeting single nucleotide mutation in PIK3CA oncogene in breast cancer-derived cells
大山隼礼, 小林芳明, UI-TEI Kumiko
日本分子生物学会年会プログラム・要旨集(Web) 45th 2022
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Molecular design of single nucleotide polymorphism-distinguishable SNPD-siRNA
程久美子, 小林芳明, 浅野吉政
日本分子生物学会年会プログラム・要旨集(Web) 45th 2022
Professional Memberships
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2019.6 - Present
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Nucleic Acids Therapeutics Society of Japan
2017.5 - Present
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The Molecular Biology Society of Japan
2016.7 - Present
Research Projects
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Molecular dynamics of siRNA-AGO complexes in complex cellular environments
Grant number：21K20630 2021.8 - 2023.3
Grants-in-Aid for Scientific Research Grant-in-Aid for Research Activity Start-up
Kobayashi Yoshiaki
Grant type：Scientific research funding
Previous biochemical analyses have revealed that small interfering RNA (siRNA) recognized target mRNAs via the seed region (nucleotides 2~8 counting from the 5´ end of siRNA), but the role of the seed region by its position was not well understood. By measuring siRNA activity in human cells, we found that the seed region of siRNA is divided into two functional domains, nucleotides 2-5 and 6-8. Nucleotides 2-5 are domains that do not contribute significantly to RNAi activity, while nucleotides 6-8 are the domains that determine the degree of RNAi activity.
FD Participation
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2024.4 Role：Participation Title：令和６年度 第１回全学FD（新任教員の研修）The 1st All-University FD (training for new faculty members) in FY2024
Organizer：University-wide