Language：English   Publishing type：Research paper (scientific journal)   Publisher：NATURE PORTFOLIO  

The development of simple and safe methods for recovering environmental pollutants, such as heavy metals, is needed for sustainable environmental management. Short elastin-like peptide (ELP) analogues conjugated with metal chelating agents are considered to be useful as metal sequestering agents as they are readily produced, environment friendly, and the metal binding domain can be selected based on any target metal of interest. Due to the temperature dependent self-assembly of ELP, the peptide-based sequestering agents can be transformed from the solution state into the particles that chelate metal ions, which can then be collected as precipitates. In this study, we developed a peptide-based sequestering agent, AADAAC-(FPGVG)(4), by introducing the metal-binding sequence AADAAC on the N-terminus of a short ELP, (FPGVG)(4). In turbidity measurements, AADAAC-(FPGVG)(4) revealed strong self-assembling ability in the presence of metal ions such as Cd2+ and Zn2+. The results from colorimetric analysis indicated that AADAAC-(FPGVG)(4) could capture Cd2+ and Zn2+. Furthermore, AADAAC-(FPGVG)(4) that bound to metal ions could be readily recycled by treatment with acidic solution without compromising its metal binding affinity. The present study indicates that the fusion of the metal-binding sequence and ELP is a useful and powerful strategy to develop cost-effective heavy metal scavenging agents with low environmental impacts.

DOI： 10.1038/s41598-022-05695-w

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DOI： https://doi.org/ 10.1371/journal.pone.0246583

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1021/acsomega.0c06140.

Language：English   Publishing type：Research paper (scientific journal)  

Halogenation of organic compounds is one the most important transformations in chemical synthesis and is used for the production of various industrial products. A variety of halogenated bisphenol analogs have recently been developed and are used as alternatives to bisphenol A (BPA), which is a raw material of polycarbonate that has adverse effects in animals. However, limited information is available on the potential toxicity of the halogenated BPA analogs. In the present study, to assess the latent toxicity of halogenated BPA analogs, we evaluated the binding and transcriptional activities of halogenated BPA analogs to the estrogen-related receptor γ(ERRγ), a nuclear receptor that contributes to the growth of nerves and sexual glands. Fluorinated BPA analogs demonstrated strong ERRγbinding potency, and inverse antagonistic activity, similar to BPA. X-ray crystallography and fragment molecular orbital (FMO) calculation revealed that a fluorine-substituted BPA analog could interact with several amino acid residues of ERRγ-LBD, strengthening the binding affinity of the analogs. The ERRγbinding affinity and transcriptional activity of the halogenated BPAs decreased with the increase in the size and number of halogen atom(s). The IC₅₀ values, determined by the competitive binding assay, correlated well with the binding energy obtained from the docking calculation, suggesting that the docking calculation could correctly estimate the ERRγbinding potency of the BPA analogs. These results confirmed that ERRγhas a ligand binding pocket that fits very well to BPA. Furthermore, this study showed that the binding affinity of the BPA analogs can be predicted by the docking calculation, indicating the importance of the calculation method in the risk assessment of halogenated compounds.

DOI： 10.1021/acs.chemrestox.9b00379

Language：English   Publishing type：Research paper (scientific journal)  

17β-Estradiol (E2) is a natural steroid ligand for the structurally and physiologically independent estrogen receptors (ERs) ERα and ERβ. We recently observed that CF₃-containing bisphenol AF (BPAF) works as an agonist for ERα but as an antagonist for ERβ. Similar results were also observed for the CCl₃-containing bisphenol designated as HPTE. Both BPAF and HPTE are comprised of a tri-halogenated methyl group in the central alkyl moiety of their bisphenol structures, which strongly suggests that halogens contribute directly to the agonist/antagonist dual biological functions. We conducted this study to investigate the structure-activity relationships by assessing together newly synthesized CF₃- and CBr₃-containing bisphenol E analogs (BPE-X). We first tested bisphenols for their receptor binding ability and then for their transcriptional activities. Halogen-containing bisphenols were found to be fully active for ERα, but almost completely inactive for ERβ. When we examined these bisphenols for their inhibitory activities for E2 in ERβ, we observed that they worked as distinct antagonists. The ascending order of agonist/antagonist dual biological functions was BPE-F < BPE-Cl (HPTE) ≤ BPAF < BPE-Br, demonstrating that the electrostatic halogen bonding effect is a major driving force of the bifunctional ERα agonist and ERβ antagonist activities of BPAF.

DOI： 10.1016/j.bmc.2019.115274

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1016/j.taap.2019.114610

Language：English   Publishing type：Research paper (scientific journal)  

DOI： https://doi.org/10.1016/j.taap.2019.114610

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1021/acs.biomac.8b00353

Language：English   Publishing type：Research paper (scientific journal)  

Language：English   Publishing type：Research paper (other academic)  

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1002/psc.2876

Language：English   Publishing type：Research paper (other academic)  

Language：Japanese   Publishing type：Research paper (international conference proceedings)  

Language：Japanese   Publishing type：Research paper (international conference proceedings)  

Language：English  

Language：English  

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Language：English  

DOI： 10.1002/anie.200905158

Language：English  

DOI： 10.3987/COM-08-S(F)93

Language：English   Publishing type：Research paper (scientific journal)  

Synthetic elastin-like peptides (ELPs) that possess characteristic tropoelastin-derived hydrophobic repetitive sequences, such as (VPGVG)n, exhibit thermoresponsive reversible self-assembly. Although their thermoresponsive properties have been well-studied, the sequence-dependent and structural requirements for self-assembly remain ambiguous. In particular, it is still unclear whether the amino acid sequences derived from tropoelastin are necessary for self-assembly. In this study, 11 sequence-shuffled ELP analogues based on (FPGVG)5, which is a previously developed short ELP (sELP), were designed to elucidate the sequence-dependent and structural requirements for their self-assembly. Among them, eight shuffled peptides exhibited self-assembling properties, whereas the other three peptides were difficult to dissolve in water. Structural analyses revealed that the structural characteristics of the three insoluble peptides were different from those of their thermoresponsive analogues. Furthermore, the secondary structures of the peptide analogues possessing the self-assembly abilities were different from each other. These results suggest that the potential for self-assembly and water solubility of sELPs depend on the primary structure in each repeated unit. Moreover, several shuffled analogues exhibited more potent self-assembling properties than the original (FPGVG)5, indicating that shorter ELPs can be obtained using their novel motifs as repetitive units. We also observed that the presence of Pro-Gly sequence in the repeating units was advantageous in terms of peptide solubility. Although further analysis will be necessary to elucidate the molecular mechanism underlying the self-assembly of these sELPs, this study provides insights into the relationship between the amino acid sequence and the self-assembling ability of the peptides for developing new sELPs for various applications.

DOI： 10.1021/acs.biochem.3c00146

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Language：Japanese   Publisher：Faculty of Arts and Science, Kyushu University  

"Experimental approach to Natural Science" is a course that aims to deepen students' interest and understanding of natural science through experiments. The class was developed with "liberal science" in mind and was first offered in the summer quarter of 2022. After all classes were completed, we conducted an interview survey of the students who took the class, asking whether the class met his/her expectations and whether the class changed his/her view of natural science. The results of the survey revealed: (1) Most of the students had expected to conduct many experiments and to learn actively in this class, and the class generally met their expectations; (2) Students became more familiar with natural science through the class and showed a positive attitude toward learning natural science. On the other hand, based on the students' attitude during the course and the results of the interviews, the following improvements could be made: (1) to emphasize training to logically construct and test hypotheses rather than acquiring new knowledge in this course; (2) to provide more detailed explanations regarding the purpose of each experiment and interpretation of the results.

DOI： 10.15017/6770262

CiNii Research

Language：English   Publishing type：Research paper (scientific journal)  

Halogenated flame retardants comprising bisphenol A (BPA) derivatives, such as tetrabromobisphenol A (TBBPA), have been studied their adverse effects on human health. However, despite the fact that these halogenated BPAs are easily degraded in the environment, the risks to living organisms due to these degraded products have mostly been overlooked. To evaluate the potential toxicity of degraded TBBPAs and related compounds, we examined the cytotoxicity of halogenated bisphenol A derivatives possessing one to four halogen atoms in vitro. The results indicated that the degraded TBBPA derivatives exhibited strong cytotoxicity against HeLa cells than TBBPA. Interestingly, the di-halogenated BPA derivatives possessing two halogen atoms exhibited the strongest cytotoxicity among tested compounds. In addition, a lactate dehydrogenase release assay, fluorescence spectroscopy and flow cytometry results indicated that dibromo-BPA and diiodo-BPA induced both apoptotic and necrotic cell death by damaging the cell membranes of HeLa cells. Moreover, Escherichia coli growth was inhibited in the presence of dehalogenated TBBPA and related compounds. These findings suggest that halogenated BPA derivatives that leak from various flame-retardant-containing products require strict monitoring, as not only TBBPA but also its degraded products in environment can exert adverse effects to human health.

DOI： 10.1016/j.heliyon.2023.e13003

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Language：English   Publishing type：Research paper (scientific journal)   Publisher：WILEY  

Elastin-like peptides (ELPs) exhibit a reversible phase transition, known as coacervation, triggered by temperature changes. This property makes them useful as stimuli-responsive molecular materials for various applications. Among ELPs, short peptide chain lengths have some advantages over long peptide chain lengths because short ELPs can be easily obtained by chemical synthesis, allowing the use of various amino acids, including D-type and unnatural amino acids, at any position in the sequence. Moreover, the incorporated amino acids readily affect the temperature-responsive behavior of ELPs. However, to be utilized in various applications, it is necessary to develop short ELPs and to investigate their temperature-responsive properties. To obtain further insights into the temperature-responsive behavior of the short ELPs, we investigated branched short ELP analogs composed of (FPGVG)(n) chains (n = 1 or 2, abbreviated as F1 and F2, respectively). We synthesized multimers composed of four F1 chains or two to four F2 chains using ethylenediaminetetraacetic acid (EDTA) as a central component of multimerization. Our results show that the multimers obtained exhibited coacervation in aqueous solutions whereas linear F1 or F2 did not. Furthermore, the structural features of the obtained multimers were the same as those of linear (FPGVG)(4). In this study, we demonstrated that molecules capable of coacervation can be obtained by multimerization of F1 or F2. The temperature-responsive molecules obtained using short ELPs make it possible to use them as easy-to-synthesize peptide tags to confer temperature responsiveness to various molecules, which will aid the development of temperature-responsive biomaterials with a wide variety of functions.

DOI： 10.1002/psc.3449

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CiNii Research

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1016/j.jbc.2021.101173

Language：English   Publishing type：Research paper (scientific journal)  

Language：English   Publishing type：Research paper (scientific journal)  

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Language：English   Publishing type：Research paper (scientific journal)  

DOI： https://doi.org/10.3390/s20174781

Language：English   Publishing type：Research paper (other academic)  

Language：English   Publishing type：Research paper (other academic)  

Language：English   Publishing type：Research paper (other academic)  

Language：English   Publishing type：Research paper (scientific journal)  

Employment of appropriate receptor conformations as templates is essential for appropriate identification of the latent receptor binding ability of chemicals using in silico docking calculations. In this study, we performed docking calculations using a number of agonist- and antagonist-bound conformations of 83 estrogen receptor (ER) α-ligand-binding domains as templates to clarify the type of receptor conformations required for reasonable identification of endocrine disruptors. Our results showed that 17β-estradiol and diethylstilbestrol (ERα agonists) bound preferentially to the agonist conformations, whereas 4-hydroxytamoxifen and raloxifene (ERα antagonists) bound selectively to the antagonist conformations. We also observed that bisphenol A analogues, which are partial agonists, bound more moderately and preferentially to the agonist conformations as compared with the antagonist conformations. Additionally, the docking calculations were able to estimate biological agonist or antagonist activity of chemicals based on the receptor conformation selectivity. Furthermore, structural analyses of the ligand-binding domains and docking calculation utilizing C-terminal-truncated receptors indicated that the C-terminal regions of these domains were capable of discriminating agonists from nonagonists. These results suggest that both agonist- and antagonist-binding conformations of receptors are necessary to predict the binding affinity and biological activity of chemicals for docking calculation-based risk assessment. Furthermore, this in silico method can be beneficial for drug discovery because it is useful for rapid searching of ligands for receptors and preventing the side effects caused by unfavorable receptor binding.

DOI： 10.1021/acsomega.9b00050

Language：English   Publishing type：Research paper (other academic)  

Language：English  

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1021/acsmedchemlett.9b00093

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1021/acs.biochem.7b01144

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1016/j.phanu.2017.09.001

Event date： 2023.3

Language：English  

Country：Japan  

Event date： 2022.11

Language：Japanese  

Venue：名古屋国際会議場   Country：Japan  

Event date： 2022.10

Language：English  

Country：Japan  

Event date： 2022.6

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：オンライン発表   Country：Japan  

Event date： 2021.11

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：オンライン発表   Country：Japan  

Event date： 2021.10

Language：English   Presentation type：Oral presentation (general)  

Country：Japan  

Event date： 2020.11

Language：Japanese   Presentation type：Oral presentation (general)  

Country：Japan  

Event date： 2020.3

Language：English   Presentation type：Oral presentation (general)  

Country：Japan  

Event date： 2019.10

Language：English   Presentation type：Oral presentation (general)  

Country：Japan  

Event date： 2019.9

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：パシフィコ横浜   Country：Japan  

弾性タンパク質エラスチンは、温度を上げると会合し下げると再び解離するという可逆的な自己集合（コアセルベーション）を示す。エラスチンは疎水性ドメインにVal-Pro-Gly-Val-Gly (VPGVG)のような特徴的な繰り返し配列を持ち、この繰り返し配列がコアセルベーションに重要であることが報告されている。また、この繰り返し配列を模倣したエラスチン由来ペプチド（Elastin-like Peptides; ELP）も同様に自己集合能を示すことから、人工血管や薬物送達システム等の基材として応用が可能なバイオマテリアル用の素材としての応用が期待されている。しかし、これらのペプチドがコアセルベーションを示す際の詳細な構造や機能、および配列に含まれる各アミノ酸残基の役割には不明な点が多く、分子素材としてELPを合理的にデザインするために、自己集合機構の解明が求められている。
代表的なELPである(VPGVG)n繰り返し配列ペプチドが自己集合を示すためには、200アミノ酸残基以上（配列繰り返し回数n>40）からなるポリペプチドが必要である。当研究室の先行研究では、繰り返し配列の先頭のVal残基をより疎水性の高いフェニルアラニン（Phe）に置換した合成ペプチド(FPGVG)nが25残基程度（n=5）の小分子でコアセルベーションを示すことを報告した。このことから、ELPがコアセルベーションを示すためには、ペプチドを構成する残基の疎水性が大きく関与していると考えられた。ところが、これの検証研究において、(FPGVG)5の中の１つの繰り返し配列における1個のPheを側鎖にヒドロキシ基を有するチロシン（Tyr）に置換したところ、親水性官能基が導入されたにも関わらずコアセルベーション能が高くなることが判明した。加えて、Tyrの置換位置の違いによってもコアセルベーション能が変化した。一方、Tyrと同様に側鎖にヒドロキシ基を有するセリン（Ser）でPheを置換すると、置換する位置に関わらず自己集合能が著しく低下した。これらの結果から、ELPの自己集合能は単純にアミノ酸の疎水性および親水性官能基の有無によって規定されるわけではないことが示唆された。そこで、さらにこれらの実験結果について分子構造から検証するため、分子動力学計算を用いて(FPGVG)5ならびにその一残基置換ペプチドの構造解析を行い、置換する残基の種類や位置によってペプチドの構造や分子内相互作用がどのように変化するか？について検証を行なった。

Event date： 2018.12

Language：English   Presentation type：Oral presentation (general)  

Country：Japan  

Event date： 2016.10

Language：Japanese   Presentation type：Oral presentation (general)  

Country：Japan  

Event date： 2016.9

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：仙台国際センター   Country：Japan  

プラスチックの原料として幅広く使用されているビスフェノールA（BPA）が、核内受容体のひとつであるエストロゲン関連受容体γ型（ERRγ）に強く結合することが判明し、その機能を撹乱することが懸念されている。このため、代替としてBPAをハロゲンなどで置換した「新世代ビスフェノール」とよばれる一連の化合物が使用されるようになったが、ハロゲン化BPAもERRγに強く結合するともいわれており、これらの物質がもつ潜在的な撹乱リスクの評価が求められている。そこで本研究では、ハロゲン化がBPAのERRγに対する受容体結合性にどのように影響するかをin vitro とin silicoの手法により調べることを目的として実験を行った。
　ハロゲン化BPAとERRγの相互作用を解析するため、まずハロゲン化BPAを化学合成し、核内受容体ERRγに対する競合結合試験を行って結合親和性を調べた。その結果、ハロゲン置換基が大きく、また置換数が多くなるほどBPAアナログの結合親和性は低くなった。続いて、BPAアナログ10種について、5種類のカラムを用いた逆相HPLCを実施し、その溶出時間からリガンドの結合における相互作用の寄与を調べた。その結果、BPAアナログとカラム担体の相互作用は、主に疎水性相互作用が重要であること、また、π電子を介した相互作用を検証したところ、BPAの芳香環の電子とカラムのアルキル基の水素によるCH-π相互作用が主であることが示唆された。これらの相互作用がBPAとERRγの相互作用においても重要であると考えられた。また、ERRγの結晶構造（PDB ID: 2E2R）を鋳型として各種ハロゲン化BPAアナログのドッキング計算を行い、結合エネルギーが最小となるリガンドの最安定構造を用いてハロゲン化BPAアナログとERRγの結合構造を解析した。その結果、ハロゲン化BPAはBPAとほぼ同じ配向でERRγに結合するが、ヨウ素一置換体のみ立体障害の影響から他のアナログと異なる結合性を示し、その結果、生理活性が変わる可能性があることが示唆された。

Event date： 2016.3

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：同志社大学京田辺キャンパス   Country：Japan  

温度依存的かつ可逆的な自己集合（コアセルベーション）を示す弾性タンパク質・エラスチンは様々な分子素材への応用が期待されており、その分子基盤となる自己凝集のメカニズムを解明することが必要とされている。当研究室ではこれまでに、エラスチン由来の合成ペプチド(FPGVG)5が短い鎖長でもエラスチンと同様の凝集特性を示すことを報告した[1]。本研究では、こうした低分子でコアセルベーションを示す合成ペプチドに蛍光基を導入してその凝集や蛍光の挙動を調べ、エラスチンの凝集機構に関する知見を得ることを目的として実験を行った。
(FPGVG)5のN末端を蛍光標識したペプチドAbz-F5を合成して凝集特性を調べたところ、コアセルベーション能は蛍光標識していない(FPGVG)5と比較して著しく高くなった。また、Abz-F5の蛍光強度は溶液の温度上昇によって緩やかに減少し、分子が集合すると大きく減少した。分子動力学計算でAbz-F5の構造解析を行った結果、ペプチドのN末端付近の環境は温度により大きく変化しないことが示唆された。このことから、温度依存的なペプチドの蛍光変化は、主に溶液の温度上昇と自己集合による分子間相互作用の増大によって生じると考えられた。

Event date： 2015.12

Language：Japanese   Presentation type：Oral presentation (general)  

Country：Japan  

弾性タンパク質・エラスチンは、温度を上げると会合し下げると再び解離するという可逆的な自己集合（コアセルベーション）を示す。この特性を利用することで、エラスチンは様々な分子素材への応用が期待されており、その分子基盤となる自己凝集のメカニズムを解明することが必要とされている。こうした中、当研究室ではこれまでに、エラスチン由来の繰り返し配列を持つ合成ペプチド(FPGVG)nが、25残基という低分子量でコアセルベーションを示すことを報告した。そこで、このような低分子でコアセルベーションを示す合成ペプチドに蛍光基を導入してそのコアセルベーションや蛍光の挙動を調べることで、エラスチンの凝集機構に関する知見が得られるのではないかと考えた。今回、 (FPGVG)nのN末端に蛍光基を導入したペプチドを合成し、コアセルベーション特性および立体構造の解析を行った。

Event date： 2015.11

Language：English   Presentation type：Oral presentation (general)  

Country：Japan  

Event date： 2015.11

Language：Japanese   Presentation type：Public lecture, seminar, tutorial, course, or other speech  

Venue：九州大学馬出キャンパス   Country：Japan  

Event date： 2015.5

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：九州大学箱崎キャンパス   Country：Japan  

Event date： 2015.3

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Event date： 2014.10

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Event date： 2014.10

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Country：Japan  

Event date： 2014.5

Language：Japanese   Presentation type：Oral presentation (general)  

Country：Japan  

Event date： 2014.3

Language：English   Presentation type：Oral presentation (general)  

Venue：つくば国際会議場（茨城県）   Country：Japan  

Event date： 2014.3

Language：Japanese   Presentation type：Oral presentation (general)  

Country：Japan  

Event date： 2013.11

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Country：Japan  

Event date： 2013.6

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：東京医科歯科大学（東京都）   Country：Japan  

Event date： 2013.3

Language：Japanese  

Country：Japan  

Event date： 2012.12

Language：Japanese  

Country：Japan  

Event date： 2012.12

Language：Japanese  

Country：Japan  

Event date： 2012.12

Language：Japanese  

Country：Japan  

Event date： 2012.9

Language：Japanese  

Country：Japan  

Event date： 2012.5

Language：Japanese  

Country：Japan  

Event date： 2012.3

Language：Japanese  

Country：Japan  

Event date： 2011.9

Language：Japanese  

Country：Japan  

Event date： 2011.9

Language：Japanese  

Country：Japan  

Event date： 2010.12 - 2016.12

Language：Japanese  

Country：Japan  

Event date： 2010.12

Language：Japanese  

Country：Japan  

Event date： 2010.8 - 2010.9

Language：Japanese  

Country：Japan  

Event date： 2010.5

Language：Japanese  

Country：Japan  

Event date： 2009.5

Language：English  

Country：Korea, Republic of  

Event date： 2008.11

Language：Japanese  

Country：Japan  

Event date： 2008.3

Language：Japanese  

Country：Japan  

Event date： 2023.10 - 2023.11

Language：Japanese  

Country：Japan  

Event date： 2021.11

Language：Japanese   Presentation type：Oral presentation (general)  

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Event date： 2021.10

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Country：Japan  

Event date： 2021.6

Language：Japanese   Presentation type：Oral presentation (general)  

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Event date： 2021.6

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：オンライン発表   Country：Japan  

Event date： 2020.5

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：誌上開催   Country：Japan  

Event date： 2019.6

Language：Japanese  

Venue：長崎大学文教キャンパス   Country：Japan  

Event date： 2019.3

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：甲南大学　岡本キャンパス   Country：Japan  

Event date： 2018.3

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：日本大学理工学部船橋キャンパス   Country：Japan  

Event date： 2017.12

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：神戸大学六甲台第2キャンパス   Country：Japan  

Event date： 2017.5

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：宮日会館/宮崎大学清武キャンパス   Country：Japan  

Event date： 2017.3

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：慶應義塾大学日吉キャンパス   Country：Japan  

弾性タンパク質・エラスチンに由来する合成ペプチド (FPGVG)n1)や(WPGVG)nは、短い鎖長でもエラスチンと同様の温度依存的かつ可逆的な自己集合を示し、様々な分子素材への応用が期待されている。本研究では、これらの芳香族アミノ酸を含むエラスチン由来ペプチドを環状化して相転移温度や凝集の挙動を調べ、エラスチンの凝集機構に関する知見を得ることを目的として実験を行った。
　(FPGVG)nを環状化したペプチドcyclo(FPGVG)nを合成して凝集特性を調べたところ、n=3-5のアナログは、直鎖(FPGVG)5と比較して著しく低濃度で自己集合を示した。一方、cyclo(WPGVG)n (n=1-3)は水溶性が低く、自己集合を観測できなかった。分子動力学計算による検証を行った結果、環状ペプチドの自己集合には、分子内に形成されるターン構造が重要であることが示唆された。また、色素を添加したcyclo(FPGVG)5水溶液を用いて顕微鏡観察および動的光散乱法による粒径測定を行ったところ、直径約3 µMのリポソーム様の膜構造の内部に色素が包含されていることが確認され、環状(FPGVG)nが薬物送達システムの基材として応用可能であることが示された。

Event date： 2016.3

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：同志社大学京田辺キャンパス   Country：Japan  

Event date： 2016.3

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：同志社大学京田辺キャンパス   Country：Japan  

Event date： 2016.3 - 2015.3

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：同志社大学京田辺キャンパス   Country：Japan  

Event date： 2016.3 - 2015.3

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：同志社大学京田辺キャンパス   Country：Japan  

Event date： 2015.12

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：自治医科大学地域医療情報研修センター   Country：Japan  

Event date： 2015.12

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：神戸ポートアイランド   Country：Japan  

Event date： 2015.11

Language：English   Presentation type：Oral presentation (general)  

Country：Japan  

Event date： 2015.6

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：北九州国際会議場   Country：Japan  

Event date： 2015.6

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：北九州国際会議場   Country：Japan  

Language：Japanese  

Language：Japanese  

Language：Japanese  

Language：Japanese  

Language：Japanese  

Type：Competition, symposium, etc. 

Number of participants：32

Type：Peer review 

Number of peer-reviewed articles in foreign language journals：2

Type：Competition, symposium, etc. 

Number of participants：49

Type：Competition, symposium, etc. 

Type：Competition, symposium, etc. 

Type：Competition, symposium, etc. 

Type：Competition, symposium, etc. 

Number of participants：50

Type：Competition, symposium, etc. 

Number of participants：68