Web of Science

Language：English   Publisher：(公社)日本医学放射線学会  

局所進行子宮頸癌(LACC)に対し、経直腸超音波(TRUS)ガイド下経腟的アプローチによる組織内照射併用腔内照射(IC/IS BT)を行い、有効性と安全性を後向きに調査した。外照射療法とTRUSガイド下で経腟アプローチによりIC/IS BTの治療を受けたLACC患者30例(年齢31～82歳)を調査対象とし、2年局所制御率(LC)、無増悪生存率(PFS)および全生存率(OS)をKaplan-Meier法で分析した。また、遅発性有害事象に基づきIC/IS BTの安全性についても評価した。その結果、経過観察期間の中央値は22ヵ月で、2年LC、PFSおよびOSはそれぞれ90%、61%、82%であった。また、IC/IS BT法に関連した重篤な合併症例はみられず、グレード3以上の遅発性有害事象としては、グレード4の結腸穿孔が認められた。以上より、経腟的アプローチによるTRUSガイド下でのIC/IS BTは、LACCにおいて、安全且つ良好な局所腫瘍制御が得られることが確認された。

Language：English   Publisher：Japanese Journal of Radiology  

Purpose: This study evaluated the efficacy and safety of transvaginal approach combined intracavitary and interstitial brachytherapy (IC/IS BT) assisted by transrectal ultrasound (TRUS) for treatment of locally advanced cervical cancer (LACC). Materials and Methods: A total of 30 patients of LACC treated with external beam radiotherapy and IC/IS BT via transvaginal approach assisted by transrectal ultrasound were observed retrospectively. The 2-year local control (LC), progression-free survival (PFS), and overall survival (OS) were analyzed using the Kaplan–Meier method. Late adverse events were also evaluated to assess the safety of IC/IS BT. Results: The median follow-up period was 22 months. The 2-year LC, PFS, and OS were 90%, 61%, and 82%, respectively. We observed no critical complications related to the IC/IS BT technique. Late adverse events of grade 3 or more included one case of grade 4 colon perforation. Conclusion: Our patient series demonstrated that radiotherapy combined with transvaginal approach, TRUS-assisted IC/IS BT achieves favorable local control and safety for LACC.

DOI： 10.1007/s11604-023-01481-4

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Scopus

PubMed

Language：English   Publisher：Scientific Reports  

Although methods for sequencing library preparation from double-stranded DNA are well established, those from single-stranded DNA (ssDNA) have not been well studied. Further, the existing methods have limitations in efficiency and yield. Therefore, we developed a highly efficient procedure for sequencing library preparation from ssDNA. In this method, the first adaptor tagging of ssDNA is performed using terminal deoxyribonucleotidyl transferase (TdT)-assisted adenylate connector-mediated ssDNA (TACS) ligation, which we reported recently. After complementary strand synthesis using the adaptor-tagged ssDNA, second adaptor tagging via Vaccinia virus topoisomerase I (VTopoI or TOPO)-based adaptor ligation is performed. With additional steps for degradation, repression, and removal of the adaptor dimer, the proposed TACS-TOPO scheme realizes adaptor dimer-free sequencing library preparation from ssDNA samples of 24 pg. The TACS-TOPO scheme was successfully applied to cell-free DNA analysis with amplification-free library preparation from 50 µL of human serum. A modified TACS-TOPO scheme was also applied to DNA extracted from ancient human bones, bringing two to eight times more library yields than those using a conventional library preparation protocol. The procedures for preparing VTopoI and its complex with a double-stranded oligonucleotide adaptor are also described. Overall, the proposed TACS-TOPO scheme can facilitate practical and sensitive sequencing analysis of ssDNA.

DOI： 10.1038/s41598-023-40890-3

Web of Science

Scopus

PubMed

Event date： 2024.11

Language：Japanese   Presentation type：Poster presentation  

Event date： 2023.11 - 2023.12

Language：Japanese   Presentation type：Oral presentation (general)  

Event date： 2021.11

Language：Japanese   Presentation type：Oral presentation (general)  

Language：Japanese  

Language：Japanese