2026/08/28 更新

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写真a

ヤマザキ　キヨウタロウ
山﨑 匡太郎
YAMAZAKI KYOTARO
所属
医学研究院 応用幹細胞医科学部門 助教
職名
助教
外部リンク

論文

  • Epigenetic landscape of synthetic human centromere core regions: CENP-A assembly and euchromatic modifications interdependently antagonize heterochromatin accumulation 査読

    Ohzeki, J; Watanabe, A; Lee, JX; Minami, C; Kugou, K; Yamazaki, K; Shirasawa, K; Isobe, S; Endo, Y; Kazuki, Y

    NUCLEIC ACIDS RESEARCH   54 ( 12 )   2026年6月

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    記述言語：英語   掲載種別：研究論文（学術雑誌）  

    DOI： 10.1093/nar/gkag597

  • High-throughput CRISPR live-cell imaging of low-frequency chromosomal events quantifies the latent efficiency of chromosome engineering

    Hu, X; Iwamoto, Y; Yamazaki, K; Kishima, N; Otaki, N; Miyamoto, H; Miyaoka, Y; Kazuki, Y; Ota, S

    BIORXIV   2026年5月

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    記述言語：英語  

    DOI： 10.64898/2026.05.10.724155

  • Generation of transchromosomic mice harboring HLA-A/B/C and human B2M via mouse artificial chromosome and triple BAC integration 査読

    Kishima, N; Moriwaki, T; Uno, N; Suzuki, T; Abe, S; Sugawara, M; Nagashima, Y; Kazuki, K; Endo, T; Yamazaki, K; Nakagawa, W; Yuno, R; Moriguchi, Y; Matsukura, S; Tomizuka, K; Kazuki, Y

    SCIENTIFIC REPORTS   15 ( 1 )   2025年7月

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    記述言語：英語   掲載種別：研究論文（学術雑誌）  

    DOI： 10.1038/s41598-025-13138-5

  • Development of an OATP1-humanized transchromosomic mouse model for prediction of hepatic drug uptake in humans. 査読 国際誌

    Koishikawa, T; Kazuki, K; Ohnishi, R; Okita, K; Mizuno, T; Abe, S; Nanchi, I; Masago, Y; Yamazaki, K; Ohzeki, JI; Kusuhara, H; Kazuki, Y

    DRUG METABOLISM AND DISPOSITION: THE BIOLOGICAL FATE OF CHEMICALS   53 ( 2 )   100028 - 100028   2025年2月

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    記述言語：英語   掲載種別：研究論文（学術雑誌）  

    DOI： 10.1016/j.dmd.2024.100028

  • Microcell-mediated chromosome transfer between non-identical human iPSCs 査読

    Uno, N; Miyamoto, H; Yamazaki, K; Egawa, M; Kobayashi, H; Kazuki, K; Osaki, M; Suzuki, T; Hamamichi, S; Oshimura, M; Tomizuka, K; Kazuki, Y

    MOLECULAR THERAPY - NUCLEIC ACIDS   35 ( 4 )   102382 - 102382   2024年12月

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    記述言語：英語   掲載種別：研究論文（学術雑誌）  

    DOI： 10.1016/j.omtn.2024.102382

  • Generation of Monosomy 21q Human iPS Cells by CRISPR/Cas9‐Mediated Interstitial Megabase Deletion 査読

    Egawa, M; Uno, N; Komazaki, R; Ohkame, Y; Yamazaki, K; Yoshimatsu, C; Ishizu, Y; Okano, Y; Miyamoto, H; Osaki, M; Suzuki, T; Hosomichi, K; Aizawa, Y; Kazuki, Y; Tomizuka, K

    GENES TO CELLS   2024年11月

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    記述言語：英語   掲載種別：研究論文（学術雑誌）  

    DOI： 10.1111/gtc.13184

  • Rapid human genomic DNA cloning into mouse artificial chromosome via direct chromosome transfer from human iPSC and CRISPR/Cas9-mediated translocation. 査読 国際誌

    Miyamoto, H; Kobayashi, H; Kishima, N; Yamazaki, K; Hamamichi, S; Uno, N; Abe, S; Hiramuki, Y; Kazuki, K; Tomizuka, K; Kazuki, Y

    NUCLEIC ACIDS RESEARCH   2024年1月

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    記述言語：英語   掲載種別：研究論文（学術雑誌）  

    DOI： 10.1093/nar/gkad1218

  • Co-treatment of CHO cells with Taxol and reversine improves micronucleation and microcell-mediated chromosome transfer efficiency 査読

    Uno, N; Satofuka, H; Miyamoto, H; Honma, K; Suzuki, T; Yamazaki, K; Ito, R; Moriwaki, T; Hamamichi, S; Tomizuka, K; Oshimura, M; Kazuki, Y

    MOLECULAR THERAPY - NUCLEIC ACIDS   2023年7月

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    記述言語：英語   掲載種別：研究論文（学術雑誌）  

    DOI： 10.1016/j.omtn.2023.07.002

  • Characterization of human anti-EpCAM antibodies for developing an antibody–drug conjugate 査読

    Satofuka, H; Wang, Y; Yamazaki, K; Hamamichi, S; Fukuhara, T; Rafique, A; Osako, N; Kanazawa, I; Endo, T; Miyake, N; Honma, K; Nagashima, Y; Hichiwa, G; Shimoya, K; Abe, S; Moriwaki, T; Murakami, Y; Gao, X; Kugoh, H; Oshimura, M; Ito, Y; Kazuki, Y

    SCIENTIFIC REPORTS   13 ( 1 )   2023年3月

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    担当区分：筆頭著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

    DOI： 10.1038/s41598-023-31263-x

  • Simultaneous loading of PCR-based multiple fragments on mouse artificial chromosome vectors in DT40 cell for gene delivery 査読 国際誌

    Yamazaki, K; Matsuo, K; Okada, A; Uno, N; Suzuki, T; Abe, S; Hamamichi, S; Kishima, N; Togai, S; Tomizuka, K; Kazuki, Y

    SCIENTIFIC REPORTS   12 ( 21790 )   1 - 14   2022年12月

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    担当区分：筆頭著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

    DOI： 10.1038/s41598-022-25959-9

  • Efficient human-like antibody repertoire and hybridoma production in trans-chromosomic mice carrying megabase-sized human immunoglobulin loci 査読 国際誌

    Satofuka, H; Abe, S; Moriwaki, T; Okada, A; Kazuki, K; Tanaka, H; Yamazaki, K; Hichiwa, G; Morimoto, K; Takayama, H; Nakayama, Y; Hatano, S; Yada, Y; Murakami, Y; Baba, Y; Oshimura, M; Tomizuka, K; Kazuki, Y

    NATURE COMMUNICATIONS   13 ( 1 )   1841   2022年4月   （ eISSN:2041-1723 ）

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    記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Nature Communications  

    Trans-chromosomic (Tc) mice carrying mini-chromosomes with megabase-sized human immunoglobulin (Ig) loci have contributed to the development of fully human therapeutic monoclonal antibodies, but mitotic instability of human mini-chromosomes in mice may limit the efficiency of hybridoma production. Here, we establish human antibody-producing Tc mice (TC-mAb mice) that stably maintain a mouse-derived, engineered chromosome containing the entire human Ig heavy and kappa chain loci in a mouse Ig-knockout background. Comprehensive, high-throughput DNA sequencing shows that the human Ig repertoire, including variable gene usage, is well recapitulated in TC-mAb mice. Despite slightly altered B cell development and a delayed immune response, TC-mAb mice have more subsets of antigen-specific plasmablast and plasma cells than wild-type mice, leading to efficient hybridoma production. Our results thus suggest that TC-mAb mice offer a valuable platform for obtaining fully human therapeutic antibodies, and a useful model for elucidating the regulation of human Ig repertoire formation.

    DOI： 10.1038/s41467-022-29421-2

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