Kyushu University Academic Staff Educational and Research Activities Database
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SHIGEHIKO TAMURA Last modified date:2023.11.28

Graduate School
Undergraduate School

 Reseacher Profiling Tool Kyushu University Pure
Academic Degree
Country of degree conferring institution (Overseas)
Field of Specialization
Molecular Cellular Biology
Total Priod of education and research career in the foreign country
Research Interests
  • Molecular analysis of peroxisomal biogenesis and its pathogenesis.
    keyword : peroxisome, AAA-ATPase, protein translocator complex, peroxisome biogenesis disorder
Academic Activities
1. Koichiro Yamashita, Shigehiko Tamura, Masanori Honsho, Hiroto Yada, Yuichi Yagita, Hidetaka Kosako, Yukio Fujiki, Mitotic phosphorylation of Pex14p regulates peroxisomal import machinery, Journal of Cell Biology, 10.1083/JCB.202001003, 219, 10, 2020.10, © 2020 Yamashita et al. Peroxisomal matrix proteins are imported into peroxisomes via membrane-bound docking/translocation machinery. One central component of this machinery is Pex14p, a peroxisomal membrane protein involved in the docking of Pex5p, the receptor for peroxisome targeting signal type 1 (PTS1). Studies in several yeast species have shown that Pex14p is phosphorylated in vivo, whereas no function has been assigned to Pex14p phosphorylation in yeast and mammalian cells. Here, we investigated peroxisomal protein import and its dynamics in mitotic mammalian cells. In mitotically arrested cells, Pex14p is phosphorylated at Ser-232, resulting in a lower import efficiency of catalase, but not the majority of proteins including canonical PTS1 proteins. Conformational change induced by the mitotic phosphorylation of Pex14p more likely increases homomeric interacting affinity and suppresses topological change of its N-terminal part, thereby giving rise to the retardation of Pex5p export in mitotic cells. Taken together, these data show that mitotic phosphorylation of Pex14p and consequent suppression of catalase import are a mechanism of protecting DNA upon nuclear envelope breakdown at mitosis..
1. Regulation of peroxisomal import by mitotic phosphorylation of Pex14p..
2. A newly identified mutation in the PEX26 gene is associated with a distinctly milder form of Zellweger spectrum disorder..
3. A newly identified mutation in the PEX26 gene is associated with a distinctly milder form of Zellweger spectrum disorder.
4. Mitotic regulation of peroxisomal import machinery by phosphorylation of Pex14p.
5. Regulation of peroxisomal import machinery by cell-cycle dependent phosphorylation of Pex14p.
Membership in Academic Society
  • The Japanese Biochemical Society
  • The Pharmaceutical Society of Japan
  • The Molecular Biology society of Japan
  • Japan Society for Cell Biology
Educational Activities
Life Science A
Cell Biology
Seminar for metabolic physiology