Books

1.	Koji Nakano, Scanning Electrochemical Microscopy Imaging of DNA Arrays for High Throughput Analysis Applications, in Modern Aspects of Electrochemistry. Applications of Electrochemistry and Nanotechnology in Biology and Medicine, Ed. by N. Eliaz., Springer, 105-142, 2011.12.

Reports

1.	Koji Nakano, Commentary in the general discussion in Faraday Discussion Vol. 149 Analysis for Healthcare Diagnostics and Theranostics, 2011.01.
2.	Biosensors for Application to Medical Diagnostics.
3.	RuiQi Zhang, Koji Hirakawa, Masaaki Katayama, Hizuru Nakajima, Nobuaki Soh, Koji Nakano, Toshihiko Imato, Flow Immunoassay Based on Sequential Injection Using Microbeads, J. Flow Injection Analysis, Vol. 23, No. 2. pp. 117 - 122, 2006.12.
4.	DNA Conjugate for Single-Molecular Electronic, Bioelectrochemical Devices.
5.	DNA Immobilization and Its Application to Gene Sensors.
6.	Can A DNA Double-Helix Be Applicable To Electroconductive Material?.

Papers

1.	Koji Nakano, Takayuki Honda, Kanako Yamasaki, Yoshiki Tanaka, Keiichi Taniguchi, Ryoichi Ishimatsu, Toshihiko Imato, Carbon quantum dots as fluorescent component in peroxyoxalate chemiluminescence for hydrogen peroxide determination, Bulletin of the Chemical Society of Japan, 10.1246/bcsj.20180095, 91, 7, 1128-1130, 2018.01, Nitrogen-doped carbon quantum dots synthesized by onepot, microwave-assisted pyrolysis of citric acid in the presence of a small number of N-doping precursors, 1, 2-ethylenediamine, were found to be involved in the chemically initiated electron exchange luminescence enabling peroxyoxalate chemiluminescence assay of hydrogen peroxide in the concentration range of 101000 μM..
2.	Koji Nakano, Shingo Hirata, Jun Horiuchi, Ryoichi Ishimatsu, Toshihiko Imato, Takeshi Onodera, Kenshi Hayashi, Synthesis and Self-Assembly of His-tag Hybrid of Substrate-Binging Short Domain in Transient Receptor Potential Vanilloid Type 1 for Vanaillin Sensing Application, Transactions of the Materials Research Society of Japan, http://doi.org/10.14723/tmrsj.40.175, 40, 2, 175-178, 2015.07.
3.	Koji Nakano, Takayuki Kimura, Yosuke Kitamura, Toshihiro Ihara, Ryoichi Ishimatsu, Toshihiko Imato, Potentiometric DNA Sensing Platform Using Redox-active DNA Probe Pair for Sandwich-type Dual Hybridization at Indicator Electrode Surface, Journal of Electroanalytical Chemistry, http://dx.doi.org/10.1016/j.jelechem.2014.03.029, 720-721, 71-75, 2014.04, A potentiometric sensing platform that enables real-time DNA hybridization detection is described. A model target DNA, t37s: 5'AAA AAA AAA AAA-(TC)2-Ts5-(TC)2-GGA GCT GGT GGC3', which consist of a dodecamer polydeoxyadenylic acid and the human K-ras oncogene, and a five-successive deoxythymidine phosphorothioate (Ts) was designed. With the gold–phosphorothioate binding, the DNA could bind the complementarily sequences to concentrate them at the gold electrode surfaces. Accordingly, a dodecamer polydeoxythymidylic acid having ferroin-moiety (T12FeP) and a ferrocene-modified complementary of K-ras (KrasFc) were synthesized. Electrochemical quartz-crystal-microbalance (QCM) using Au-sputtered quartz chips that served as the indicator electrode collected the electrode responses. When the electrode surface was treated with the T12FeP-hybridizad t37s, which were subsequently oxidized to the corresponding Fe(III) form, the emf developed in a buffer solution responded to KrasFc; with the electrode-attached t37s the redox-active DNAs could group into a pair to establish specific redox-titration equilibrium. A QCM confirmed the on-electrode titrimetry feasible by determining the initial concentration and the amount of hybridization along with the emf measurements. Although the present method is necessary for two-kinds of redox conjugation of the target DNA, it should be important as a novel framework of electrochemical gene sensing. Preliminary examples of real-time measurement were also demonstrated with the results of kinetics analysis data..
4.	Hisao Yoshinaga, Koji Nakano, Nobuaki Soh, Ishimatsu Ryoichi, Toshihiko Imato, A Pivot-Hinge-Style DNA Immobilization Method with Adaptable Surface Concentration Based on Oligodeoxynucleotide-Phosphorothioate Chemisorption on Gold Surfaces, Anal. Sci., 28, 11, 1059-1064, 2012.11.
5.	Hisao Yoshinaga, Koji Nakano, Nobuaki Soh, Toshihiko Imato, AFM-Imaging Diagonosis Method for Single Nucleotide Polymorphism Using Molecular Beacon DNA as an Intramolecullar Ligation Template of Target DNA and a Viewable Indicator, Anal. Sci., 28, 10, 939-945, 2012.10.
6.	北岡 桃子, Masayuki Mitsumori, Kounosuke Hayashi, Yoshiyuki Hiraishi, Hisao Yoshinaga, Koji Nakano, Katsuyuki Miyawaki, Sumihare Noji, Masahiro Goto, Noriho Kamiya, Transglutaminase-Mediated in Situ Hybridization (TransISH) System: A New Methodology for Simplified mRNA Detection, Anal. Chem., 84, 14, 5885-5891, 2012.07.
7.	Josui Shimada, Tatsuo Maruyama, 北岡 桃子, Hisao Yoshinaga, Koji Nakano, Noriho Kamiya, Masahiro Goto, Programmable protein–protein conjugation via DNA-based self-assembly, Chem. Commun., 48, 50, 6226-6228, 2012.06.
8.	Koji Nakano, Hirokazu Yamanouchi, Hisao Yoshinaga, Nobuaki Soh, Toshihiko Imato, Label-free DNA Detection Platform Based on Atomic Force Microscopy Visualisation: Characterising the Molecular-Recognition-Triggered Conformational Changes of an Immobilised Receptor Oligonucleotide Probe, Chem. Commun., 46, 13, 5683-5685, 2010.06.
9.	Koji Nakano, Yosuke Katsumi, Nobuaki Soh, Toshihiko Imato, An Atomic Force Microscopy Assay of Intercalation Binding, Unwinding and Elongation of DNA, Using a Water-Soluble Psoralen Derivative as a Covalent Binding Probe Molecule, Bull. Chem. Soc. Jpn, 2010, 3, 273-275, 2010.03.
10.	Koji NAKANO, Hideshi MATSUNAGA, Masaharu MURATA, Nobuaki SOH, Toshihiko IMATO, Synthesis Of Circular Double-Stranded DNA Having Single-Stranded Recognition Sequence As Molecular-Physical Probe For Nucleic Acid Hybridization Detection Based On Atomic Force Microscopy Imaging, Anal. Sci., 2009.08.
11.	K. Nakano, K. Nakamura, K. Iwamoto, N. Soh, T. Imato, Positive-feedback-mode scanning electrochemical microscopy imaging of redox-active DNA-poly(1,4-benzoquinone) conjugate deposited on carbon electrode for micrometer-sized hybridization biosensor applications, J. Electroanal. Chem., Vol. 628, No. 1, pp. 113–118 , 2009.02.
12.	K. Nakano, K. Ohkubo, H. Taira, M. Takagi, N. Soh, T. Imato, Synthesis of 1,4-hydroquinone-terminated alkanethiol and self-assembly on gold as characterized by interfacial electrochemistry, electrocatalysis application and ab initio calculation based on comparison with catechol-presenting analogue., J. Electroanal. Chem., Vol. 623, No. 1, pp. 49-53, 2008.11.
13.	K. Nakano, K. Ohkubo, H. Taira, M. Takagi, T. Imato, Electrocatalytic oxidation of dihydronicotineamide adenine dinucleotide on gold electrode modified with catechol-terminated alkanethiol self-assembly, Anal. Chim. Acta, Vol. 619, No. 1, pp. 30–36, 2008.06.
14.	N. Soh, K. Makihara, T. Ariyoshi, D. Seto, T. Maki, H. Nakajima, K. Nakano, T. Imato, Phospholipid-linked Coumarin: A Fluorescent Probe for Sensing Hydroxyl Radicals in Lipid Membrane, Analytica Sciences, Vol. 24, No. 2, pp. 293–296, 2008.02.
15.	N. Soh, K. Yoshida, H. Nakajima, K. Nakano, T. Imato, T. Fukaminato, M. Irie, A fluorescent photochromic compound for labeling biomolecules, Chemical Communications, Vol. 2007, No. 28, pp. 5206–5208, 2007.12.
16.	N. Soh, T. Ariyoshi, T. Fukaminato, H. Nakajima, K. Nakano, T. Imato, Swallow-tailed perylene derivative: a new tool for fluorescent imaging of lipid hydroperoxides, Organic Bimolecular Chemistry, Vol. 5, No. 23, pp. 3762–3768, 2007.12.
17.	RuiQi Zhang, Hizuru Nakajima, Nobuaki Soh, Koji Nakano, Takashi Masadome, Kazumi Nagata, Kazuhira Sakamoto, Toshihiko Imato, Sequential Injection Chemiluminescence Immunoassay for Nonionic Surfactants Using Magnetic Microbeads, Analytica Chimica Acta, Vol. 600, No. 1, pp. 105–113, 2007.09.
18.	Koji Nakano, Tadateru Yoshitake, Yasunori Yamashita, Edmond F. Bowden, Cytochrome c Self-Assembly on Alkanethiol Monolayer Electrodes as Characterized by AFM, IR, QCM, and Direct Electrochemistry, Langmuir, 23, 11, 6270-6275, 2007.05.
19.	Yan LI, Jujie REN, Hizuru NAKAJIMA, Nobuaki SOH, Koji NAKANO, Toshihiko IMATO, Surface Plasmon Resonance Immunosensor for IgE Analysis Using Two Types of Anti-IgE Antibodies with Different Active Recognition Sites, Analytical Sciences, Vol. 23, No. 1, pp. 31 ? 38., 2007.01.
20.	Koji Nakano, Electrical Transport Through DNA-Ferrocene Conjugate as Characterized by Conductive AFM Measurement, Proceedings of The 2nd International Symposium on Functional Innovation of Molecular Informatics, pp. 27, 2006.11.
21.	K. Nakano, G. Hirayama, M. Toguchi, K. Nakamura, K. Iwamoto, N. Soh, T. Imato, Poly(hydroquinone)-coated electrode for immobilizing of 5’-amine functioned capture probe DNA and electrochemical response to DNA hybridization, Science and Technology of Advanced Materials, Vol. 7, No. 7, pp. 718 - 725., 2006.10.
22.	K. Nakano, H. Matsunaga, K. Sai, N. Soh, T. Imato, Photoactive, covalent attachment of DNA on gold with double-strand specificity using self-assembled monolayers containing psoralen, Anal. Chim. Acta, Vol. 578, No. 1, pp.93 - 99, 2006.09.
23.	Y. LI, M. KOBAYASHI, K. FURUI, N. SOH, K. NAKANO, T. IMATO, Surface plasmon resonance immunosensor for histamine based on an indirect competitive immunoreaction, Analytica Chimica Acta, Vol. 576, No. 1, pp. 77 - 83, 2006.08.
24.	Nobuaki Soh, Daisuke Seto, Koji Nakano, Toshihiko Imato, Novel fuorescent probe for detecting hydroperoxides with strong emission in the visible range, Molecular Biosystems, Vol. 2, No. 2, pp. 81 - 84, 2006.06.
25.	K. Hirakawa, M. Katayama, N. Soh, K. Nakano, H. Ohura, S. Yamasaki, T. Imato, Electrochemical Sandwich Immunoassay for Vitellogenin by Sequential Injection Analysis Using Antibody Immobilized Magnetic Microbeads, Electroanalysis, Vol. 18, No. 13-14, pp. 1297 - 1305, 2006.06.
26.	N. SOH, D. SETO, K. NAKANO, T. IMATO, Methodology of reversible protein labeling for ratiometric fluorescent measurement, Molecular BioSystems, Vol. 2, No. 2, pp. 128 - 131, 2006.02.
27.	K. HIRAKAWA, M. KATAYAMA, N. SOH, K. NAKANO, T. IMATO, Electrochemical Immunoassay for Vitellogenin Based on Sequential Injection Using Antigen-immobilized Magnetic Microbeads, Analytical Sciences, Vol 22, No. 2, pp.81 - 86, 2006.01.
28.	Koji NAKANO, Hideshi MATSUNAGA, Keisuke SAI, Covalent attachment of DNA with double-strand specificity using self-assembled monolayers from psoralen, Proceedings of The 2005 International Chemical Congress of Pacific Basin Societies, pp. 366, 2005.12.
29.	Hideshi MATSUNAGA, Koji NAKANO, Nobuaki SOH, Toshihiko IMATO, Preparation, Characterization and Atomic Force Microscopic Imaging of New DNA Ligand for Single Molecular Hybridization Assay, Proceedings of The 2005 International Chemical Congress of Pacific Basin Societies, pp. 968, 2005.12.
30.	Koji NAKANO, Go HIRAYAMA, Mikiko TOGUCHI, Kaori NAKAMURA, Kaori IWAMOTO, Nobuaki SOH, Toshihiko IMATO, DNA Conjugate Electrode Having DNA/Oligo(1,4-hydroquinone)/PG Interfacial Structure for Biosensor Applications: Characterization by Electrochemical Measurements, QCM Analysis, and AFM Imaging, Program & Abstracts for the 8th Asian Conference on Analytical Sciences, pp. 138, 2005.10.
31.	R-Q. Zhang, K. Hirakawa, D. Seto, N. Soh, K. Nakano, T. Masadome, K. Nagata, K. Sakamoto, T. Imato, Sequential injection chemiluminescence immunoassay for anionic surfactants using magnetic microbeads immobilized with an antibody., Talanta, 10.1016/j.talanta.2005.07.012, 68, 2, 231-238, VOL. 68, No. 2, pp. 231 - 238, 2005.10.
32.	T. TSURUTA, Y. KATSUMI, S. SHIRAKAWA, S. TAGUCHI, K. NAKANO, Synthesis of New Psoralen Derivatives for Chemical Modification and Functionalization of DNA Duplexes as Characterized by Gel Electrophoresis, IR, QCM, Electrochemistry, and AFM, Abstract Book of International Symposium on Nano-organization and Function, 2004.11.
33.	H. MATSUNGAE, K. SAI, K. NAKANO, Covalent Attachment of Double-Stranded DNA using Self-Assembled Monolayers Containing Psoralen as Characterized by XPS, IR, QCM, Electrochemistry, and AFM, Abstract Book of International Symposium on Nano-organization and Function, pp. 48, 2004.11.
34.	K. NAKANO, K. DOI, K. TAMURA, Y. KATSUMI, Monolayer Formation of Glucose Oxidase Covalently Attached on ω-Aminoalkanethiol/Au via Activation of Carbohydrate Residue of GOx as Characterized by AFM, IR, QCM, and Biosensor Applications, Abstract of The 7th Asian Conference on Analytical Sciences, pp. 308, 2004.10.
35.	T. Anada, H. Matsunaga, R. Karinaga, K. Koumoto, M. Mizu, K. Nakano, S. Shinkai, and K. Sakuraia, Proposal of new modification technique for linear double-stranded DNAs using the polysaccharide schizopyllan, Bioorganic & Medicinal Chemistry Letters, 10.1016/j.bmcl.2004.08.043, 14, 22, 5655-5659, Vol.14, pp.5655 - 5659, 2004.09.
36.	K. Nakano, S. Shirakawa, S. Taguchi, A psoralen Derivative Containing Ferrocene for Redox-labeling of DNA and Electrochemical Gene Sensor Applications, Chemcial Sensors, Vol. 20, Supplement B, pp. 778 - 779., 2004.07.
37.	Koji Nakano, DNA-Ferrocene Conjugate for Nanoelectronic Material Applications. Atomic Force Microscope-Based, Direct Measurement of Electrical Transport through DNA Molecules, Second 21st Century COE “Towards Creating New Industries Based on Inter-Nanoscience” and 7th SANKEN International Symposium on “Hybridization of Chemistry, Biology, and Material Science”, pp. 38 - 39, 2004.01.
38.	K. Nakano, K. Doi, K. Tamura, Y. Katsumi, Self-Assembling Monolayer Formation of Glucose Oxidase and Cytochrome c Covalently Attached on Alkanethiol Monolayers on Gold, Biomolecular Chemistry. Proceedings of the ISBC 2003, pp. 284 - 285, 2003.12.
39.	K. NAKANO, K. DOI, K. TAMURA, Y. KATSUMI, Self-assembling Monolayer Formation of Glucose Oxidase Covalently Attached on 11-Aminoundecanethiol Monolayers on Gold, Chemical Communications, 10.1039/b303298a, 13, 1544-1545, Vol. 2003, No. 13, pp. 1544 - 1545, 2003.06.

Works, Software and Database

1.	Book cover of Bunseki for 2005 volume..

Presentations

1.	Koji Nakano, Junich Tanabe, Yusuke Sezaki, Ryosuke Kobayashi, Ryoichi Ishimatsu, PNAzyme: Monolithic peptide nucleic acid peptide sequence hybrid functioning as molecular recognition catalyst element for nucleic acids analysis, 2021 International Chemical Congress of Pacific Basin Societies, 2021.12.
2.	Koji Nakano, Junichi Tanabe, Ryutaro Hirata, Ryoichi Ishimatsu, Toshihiko Imato, Total chemical synthesis of microperoxidase 11 and its variants for bioanalytical and bioelectrochemical purposes, International Congress on Alanytical Sciences (ICAS2017), 2017.05.
3.	Kanako Yamasaki, Yoshiyuki Honda, Koji Nakano, Ryoichi Ishimatsu, Toshihiko Imato, Research of surface-functionalization using FMOC protection-deprotection of N-doped carbon quantum dots and application for fluorescence analysis, The 13th Asian Conference on Alanytical Sciences (ASIANALYSIS XIII), 2016.12.
4.	Yoshiki Tanaka, Koji Nakano, Ryoichi Ishimatsu, Toshihiko Imato, Synthesis of new pyrrole-imidazole polyamide as base-sequence selective minor-groove binder for application to fluorescent DNA probe, The 13th Asian Conference on Alanytical Sciences (ASIANALYSIS XIII), 2016.12.
5.	Koji Nakano, Jun Horiuchi, Shingo Hirata, Ryoichi Ishimatsu, Toshihiko Imato, Design and synthesis of artificial mimics of transient receptor potential vanilloid type 1 channel protein for chemical pain sensing, The 13th Asian Conference on Alanytical Sciences (ASIANALYSIS XIII), 2016.12.
6.	Jun Horiuchi, Koji Nakano, Shingo Hirata, Ryoichi Ishimatsu, Toshihiko Imato, Synthesis of functional hybrid proteins consist of substrate-binging domain in transient receptor potential vanilloid type 1 for biosensing applications, The 28th International Symposium on Chemical Engineering (ISChE 2015), 2015.12.
7.	Koji Nakano, Shingo Hirata, Jun Horiuchi, Ryoichi Ishimatsu, Toshihiko Imato, Takeshi Onodera, Kenshi Hayashi, Self-Assembly of Vanilloid-Binging Domain in Transient Receptor Potential Cation Channels based on His-Tag Chemistry at Gold Surfaces and Guest-Molecule Responses, The 15th IUMRS-International Conference in Asia (IUMRS-ICA 2014), 2014.08.
8.	Ｓｈｉｎｇｏ　Ｈｉｒａｔａ, Koji Nakano, Ryoichi Ishimatsu, Toshihiko Imato, Kenshi Hayashi, Synthesis of His-Tag Fused Vanilloid Receptor in Transient Receptor Potential Channels for Biosensing Application of Vanilloids, The Twelfth Asian Conference on Analytical Sciences (ASIANALYSIS XII), 2013.08.
9.	AFM Imaging of Molecular Beacon DNA Sequence and Single-Molecular Detection of DNA Hybridization.
10.	AFM Imaging of Molecular Beacon DNA Sequence and Single-Molecular Detection of DNA Hybridization.
11.	AFM Imaging of DNA for Application to Single-Molecular Biosaasy.
12.	Development of Molecular Probe for NO Radical Monitoring in Biosystem.
13.	Electrochemical DNA Sensor and its 2D Imaging by Scanning Electrochemical Microscope.
14.	DNA Immobilization on Self-Assembling Monolayer Membrane Prepared by Chemical Vapor Adsorption Method: Characterization by XPS and Application to Hybridization Assay using AFM.
15.	DNA Ligand - A New Strategy of DNA Probe for Single-Molecular Gene Assay.
16.	Single-Molecular Imaging of DNA Conjugate for Nano-, Bio-Science Applications.
17.	Single-Molecular Imaging and Direct Electrochemistry of Cytochrome c Immobilized on Self-Assembled Monolayer Electrode.
18.	Single-Molecular Imaging for Proteins and Enzyme Molecules that Covalently Immobilized on Alkanthiols Monolayer Electrodes.
19.	Single-Molecular Imaging for Proteins and Enzyme Molecules that Covalently Immobilized on Alkanthiols Monolayer Electrodes and Application to Biosensors.
20.	Characterization for Self-Assembly of Glucose Oxidase Molecules upon Covalent Immobilization on Alkanethiol Monolayer Electrode usign Partial Oxidation of Suger Residue and Shiff-Base Formation..

Educational activities in the Applied Chemistry Course, Graduate School of Engineering, Kyushu University
Electroanalytical Chemistry

Educational activities in the Functional Materials Chemistry Course, School of Engineering, Kyushu University
Physical Chemistry III
Exercises in Quantum Chemistry
Chemical English
Security/Safety in Chemical Experiments
Green Chemistry
Laboratory Experiments I ＆II

Educational activities in the KIKAN Education, Kyushu University
Basic Chemical Thermodynamics
Inorganic Materials Chemistry
Kadai-Kyogaku


Others
Japanese translation publication of English textbooks
Analytical Chemistry 7th Edition. Christian, G. D.; Dasgupta, P. K.; Schug, K. A. Wile, 2014.