| 1. |
高松 洋，野口博司，安福規之，田中將己，中野幸二，柁川一弘，津守不二夫，田井 明, 高校生を工学に惹きつけるための工学教育コンテンツ, 工学教育，65巻， 64-67, 2020.05. |
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Shuai Guo, Ryoichi Ishimatsu, Koji Nakano, Toshihiko Imato, Application of Carbon Quantum Dots in Electrogenerated Chemiluminescence Sensors, Journal of Flow Injection Analysis, Vol. 32, No. 2, pp. 75-80, https://doi.org/10.24688/jfia.32.2_75, 2015.12, Carbon quantum dots (CQDs) are a new class of carbon nanomaterials with a size lower than 10 nm, and gain more and more attention since the discovery in 2004 due to the specific characteristics such as high solubility, robust chemical inertness, convenient for modification and high resistance to photobleaching. Compared with the traditional semiconductor quantum dots, CQDs have superior biological advantages such as low toxicity and good biocompatibility. Furthermore, CQDs have the excellent electronic properties to work as electron donors and acceptors, making this kind of material a kind of ideal emitters in the electrogenerated chemiluminescence process. In this review, we describe the recent progress in the field of electrogenerated chemiluminescence sensors using CQDs as emitters or enhancers, and in the applications of protein detection, cancer marker measurement and cell counting.. |
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Rong Liu, Ryoichi Ishimatsu, Koji Nakano, Toshihiko Imato, Optical Sensing Systems Suitable for Flow Analysis on Microchips, Journal of Flow Injection Analysis, Vol. 30, No. 1, pp. 15-20, 10.24688/jfia.30.1_15, 2013.12, The interest in downsized optical sensing systems suitable for microflow analysis on microchips has increased considerably due to the fact that analysis on the microchip has many advantages such as analytical time including reaction time is short, less consumption of reactants and separation time is also short. The analytical system built on the microchip is portable and suitable for on-site analysis for environmental analysis and bed-side diagnosis for clinical analysis. These systems are miniature microanalysis labs fabricated on a single substrate, and have numerous applications in chemistry and life science. This review focuses on the recent advances in the optical sensing systems used for microfluidic devices including fluorescence detection, absorbance detection and chemiluminescence (CL) detection, especially on the system based on the organic light-emitting diodes (OLED) and optical photodiodes (OPD). In this review, we demonstrate the developments and applications of the downsized optical sensing systems and point out exciting new approaches, and provide future tendency on this field.. |
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Nobuaki Soh, Mayumi Tanaka, Koji Hirakawa, RuiQi Zhang, Hizuru Nakajima, Koji Nakano, Toshihiko Imato, Sequential Injection Immunoassay for Environmental Measurements, Analytical Sciences, Vol. 27, No, 11, pp. 1069-1076, https://doi.org/10.2116/analsci.27.1069, 2011.11, Sequential injection immunoassay systems for environmental measurements based on the selective immunoreaction between antigen and antibody were described. A sequential injection analysis (SIA) technique is suitable to be applied for the procedure of enzyme-linked immunosorbent assay (ELISA), because the washing and the addition of reagent solutions can be automated by using a computer-controlled syringe pump and switching valve. We selected vitellogenin (Vg), which is a biomarker for evaluating environmental risk caused by endocrine-disrupting chemicals in the hydrosphere, and linear alkylbenzene sulfonates (LAS) and alkylphenol polyethoxylates (APEO), which are versatile surfactants, as target analytes in the flow immunoassay systems. For Vg monitoring, SIA systems based on spectrophotometric, chemiluminescence, and electrochemical determinations were constructed. On the other hand, chemiluminescence determination was applied to the detection of LAS and APEO. For APEO, an SIA system combined with surface plasmon resonance (SPR) sensor was also developed. These new sequential injection immunoassay systems are expected to be useful systems for environmental analysis.. |
| 5. |
中野幸二, 原子間力顕微鏡（AFM）で生体分子を見る, 化学と工業，第64巻第6号，460-461頁, 2011.06. |
| 6. |
Koji Nakano, Commentary in the general discussion in Faraday Discussion Vol. 149 Analysis for Healthcare Diagnostics and Theranostics, 2011.01. |
| 7. |
中野幸二, バイオセンサーと医療診断, ぶんせき, 2007.10. |
| 8. |
RuiQi Zhang, Koji Hirakawa, Masaaki Katayama, Hizuru Nakajima, Nobuaki Soh, Koji Nakano, Toshihiko Imato, Flow Immunoassay Based on Sequential Injection Using Microbeads, J. Flow Injection Analysis, https://doi.org/10.24688/jfia.23.2_117, Vol. 23, No. 2. pp. 117 - 122, 2006.12, A flow immunoassay based on a combined technique of the sequential injection with the beads injection using magnetic microbeads immobilized with antigen or antibody is described. A methodology of the present immunoassay based on chemiluminescence and electrochemical detection and its application to the determination of vitelloginin (Vg) and anionic surfactant, linear alkylbenzene sulfonate (LAS) are described. Magnetic microbeads coated with agarose gel and polylactic acid were used for immobilization of an anti-Vg antibody and an anti-LAS antibody or Vg by a conventional amino coupling method. The introduction, trapping and flushing out of the magnetic microbeads in the immunoreaction cell were controlled by the magnet and the flow of the carrier solution. The protocol of the immunoassay in the immunoreaction cell, introduction of an analyte sample, the enzyme-labeled secondary antibody or antigen and a substrate solution for chemiluminescence or electrochemical detection were sequentially carried out by the sequential injection technique. A lower detection limit around ppb level was achieved for immunoassay for Vg and LAS. The time required for an analysis was ca 15 min/sample including incubation time for immunoreaction.. |
| 9. |
中野幸二, DNA二重らせんを電子機能・構造単位とする単一分子素子, Japan NanoNet Bulletin, 第1巻，第10号，13頁, 2004.01. |
| 10. |
中野幸二, DNAの固定化と遺伝子センサーへの応用, 表面科学, https://doi.org/10.1380/jsssj.25.744, 第25巻，第12号，744 - 751頁, 2004.12, Chemical derivatization of the terminal phosphate group of DNA, as well as the backbone linker phosphates, with sulfur-containing functional groups were developed for covalent attachment of DNA on gold surfaces via chemisorption. Results of IR measurements and quartz crystal microbalance analyses for the DNA-modified gold substrates were described and the immobilization chemistry of the present system was discussed. Next, the techniques of DNA immobilization were applied to develop a biosensor that can discriminate and respond to specific genes (gene sensor). Two examples of gene sensors both of which were coupled with electrochemical signal readouts have been successfully introduced; the first example of gene sensor was taking advantage of a redox-active psoralen compound that binds covalently with DNA and the next was achieved by the use of redox active surface adlayer having a DNA/ferrocene/Au interfacial structure. The basic feasibility of the gene sensor was also discussed.. |
| 11. |
中野幸二, DNAは電子材料になるのか？, 化学, 第24巻，第1号，34 - 35頁, 2003.12. |
