| 1. |
Ye Zhang, Chizumi Abe, Koji Ochiai, Toshiro Matsui, Tissue distribution of orally administered prenylated isoflavones, glyceollins, in Sprague-Dawley rats, J. Agric. Food Chem., 69, 49, 15165-15174, 2021.12, Apart from the physiological effects of glyceollins, information regarding their tissue distribution is scarce in the literature. Thus, the aim of this study is to clarify the distribution of glyceollins in rat organs. Glyceollins I and III were orally administered to Sprague-Dawley rats (1.0 mg/kg) with daidzein as control, and their accumulations in organs were investigated by liquid chromatography-time-of-flight/mass spectrometry (LC-TOF/MS). Glyceollins accumulated in intact and conjugated forms in circulatory organs with a Tmax of 0.5 h, in the following order of descending preference: liver, kidney, heart, lung, soleus muscle, and abdominal aorta. The accumulation of hydrophobic glyceollin I was more than 1.5 times higher than that of III. In contrast, daidzein and hydroxy equol were detected only in the liver and kidneys at lower concentrations (1/100 times) than those of glyceollins. In conclusion, prenylated isoflavones, glyceollins, were preferentially distributed in circulatory organs as intact, sulfated, or glucuronidated forms up to 6 h after the intake.. |
| 2. |
Xiaojing Sheng, Mitsuru Tanaka, Risa Katagihara, Marika Hashimoto, Satoshi Nagaoka, Toshiro Matsui, A novel approach for simultaneous analysis of peptide metabolites from orally administered glycinin in rat bloodstream by a coumarin-tagged MALDI-MS, J. Agric. Food Chem., 69, 49, 14840-14848, 2021.12. |
| 3. |
Yuna Lee, Akihiro Nakano, Saya Nakamura, Kenta Sakai, Mitsuru Tanaka, Keisuke Sanematsu, Noriatsu Shigemura, Toshiro Matsui, In vitro and in silico characterization of adiponectin-receptor agonist dipeptides, npj Science of Food, 5, 29, 2021.11, The aim of this study is to develop a dipeptide showing an adiponectin receptor 1 (AdipoR1) agonistic effect in skeletal muscle L6 myotubes. Based on the structure of the AdipoR1 agonist, AdipoRon, 15 synthetic dipeptides were targeted to promote glucose uptake in L6 myotubes. Tyr-Pro showed a significant increase in glucose uptake among the dipeptides, while other dipeptides, including Pro-Tyr, failed to exert this effect. Tyr-Pro induces glucose transporter 4 (Glut4) expression in the plasma membrane, along with adenosine monophosphate-activated protein kinase (AMPK) activation. In AdipoR1-knocked down cells, the promotion by Tyr-Pro was ameliorated, indicating that Tyr-Pro may directly interact with AdipoR1 as an agonist, followed by the activation ofAMPK/Glut4 translocation in L6 myotubes. Molecular dynamics simulations revealed that a Tyr-Pro molecule was stably positioned in the two potential binding pockets (sites 1 and 2) of the seven-transmembrane receptor, AdipoR1, anchored in a virtual 1- palmitoyl-2-oleoyl-phosphatidylcholine membrane. In conclusion, we demonstrated the antidiabetic function of the Tyr-Pro dipeptide as a possible AdipoR1 agonist..  |
| 4. |
Baorui Li, Lei Fu, Ruchia Kojima, Ayaka Yamamoto, Tomoya Ueno, Toshiro Matsui, Theaflavins prevent the onset of diabetes through ameliorating glucose tolerance mediated by promoted incretin secretion in spontaneous diabetic Torii rats, J. Funct. Foods, 86, 104702, 2021.11. |
| 5. |
Kazunori Nakagawa, Mitsuru Tanaka, Tae Hun Hahm, Huu-Nghi Nguyen, Toshiro Matsui, Yong-Xiang Chen, Yutaka Nakashima, Accumulation of plasma-derived lipids in the lipid core and necrotic core of human atheroma: imaging mass spectrometry and histopathological analyses., Arterioscler. Thoromb. Vasc. Biol., 41, 11, e498-e511, 2021.11, OBJECTIVE: To clarify the pathogenesis of human atheroma, the origin of deposited lipids, the developmental mechanism of liponecrotic tissue, and the significance of the oxidation of phospholipids were investigated using mass spectrometry-aided
imaging and immunohistochemistry.
APPROACH AND RESULTS: Atherosclerotic lesions in human coronary arteries were divided into 3 groups: pathologic intimal thickening with lipid pool, atheroma with lipid core, and atheroma with necrotic core. The lipid pool and lipid core were characterized by the deposition of extracellular lipids. The necrotic core comprised extracellular lipids and liponecrotic tissue. The proportion of cholesteryl linoleate in cholesteryl linoleate+cholesteryl oleate fraction in the extracellular lipid and liponecrotic regions differed significantly from that of the macrophage foam cell–dominant region, and the plasma-derived components (apolipoprotein B and fibrinogen) were localized in the regions. The liponecrotic region was devoid of elastic and collagen fibers and accompanied by macrophage infiltration in the surrounding tissue. Non–oxidized phospholipid (Non-OxPL), OxPL, and Mox macrophages were detected in the three lesions. In the atheroma with lipid core and atheroma with necrotic core, non-OxPL tended to localize in the superficial layer, whereas OxPL was distributed evenly. Mox macrophages were colocalized with OxPL epitopes.
CONCLUSIONS: In human atherosclerosis, plasma-derived lipids accumulate to form the lipid pool of pathologic intimal thickening, lipid core of atheroma with lipid core, and necrotic core of atheroma with necrotic core. The liponecrotic tissue in
the necrotic core appears to be developed by the loss of elastic and collagen fibers. Non-OxPL in the accumulated lipids is oxidized to form OxPL, which may contribute to the lesion development through Mox macrophages. |
| 6. |
Chizumi Abe, Ye Zhang, Kazuhiro Takao, Kuni Sasaki, Koji Ochiai, Toshiro Matsui, Visualization analysis of glyceollin production in germinating soybeans by matrix-assisted laser desorption/ionization mass spectrometric imaging technique. J. Agric., J. Agric. Food Chem., 69, 25, 7057-7063, 2021.06. |
| 7. |
Weilin Shen, Kiyomi Ono, Mitsuru Tanaka, Toshiro Matsui, Characteristics of electrospray-ionization detection of synthetic di- to penta-oligopeptides by amine derivatizations, Anal. Sci., 37, 11, 1629-1632, 2021.11. |
| 8. |
Reiko Nakao, Weilin Shen, Yasuka Shimajiri, Kumiko Kainou, Yuki Sato, Anayt Ulla, Kohta Ohnishi, Miyuki Ninomiya, Ayako Ohno, Takayuki Uchida, Mitsuru Tanaka, Kazuhito Akama, Toshiro Matsui, Takeshi Nikawa, Oral intake of rice overexpressing ubiquitin ligase inhibitory pentapeptide prevents atrophy in denervated skeletal muscle, npj Science of Food, 5, 25, 2021.09. |
| 9. |
Juneha Bak, Yoshiyuki Miyazaki, Hayato Nakano, Toshiro Matsui, Profiling sulfate content of polysaccharides in seaweed species using a ligand-assisted 1H-NMR assay, Food Sci. Technol. Res., 27, 3, 505-510, 2021.06. |
| 10. |
Xiaojing Sheng, Satoshi Nagaoka, Marika Hashimoto, Yusuke Amiya, Masato Beppu, Kenichiro Tsukamoto, Emiko Yanase, Mitsuru Tanaka, and Toshiro Matsui, Identification of peptides in blood following oral administration of β-conglycinin to Wistar rats, Food Chemistry, 10.1016/j.foodchem.2020.128197, 2021.01, In this study, β-conglycinin (100 mg/kg) was orally administered to Wistar rats in order to identify peptides that may be derived from the protein in the blood. Plasma samples taken from the tail vein up to 8 h after admin- istration were analyzed by matrix-assisted laser desorption/ionization (MALDI) and liquid chromatography- time-of-flight (LC-TOF) mass spectrometry (MS). In total, 126 signals were detected by MALDI-MS. Among the signals, nine oligopeptides (SEL, KGPL, SILGA, DSEL, GDANI, SYFV, CLQSC, GEQPRPF, and LVINEGDA) were successfully identified as β-conglycinin-derived peptides by LC-TOF/MS at a plasma concentration of 0.75–756 pmol/mL. The results demonstrated that β-conglycinin could be the dietary source protein for the oligopeptides produced prior to entering the circulating bloodstream of rats.. |
| 11. |
Tae Hun Hahm, Mitsuru Tanaka, Huu-Nghi Nguyen, Ayaka Tsutsumi, Koichi Aizawa, and Toshiro Matsui, Matrix-assisted laser desorption/ionization mass spectrometry-guided visualization analysis of intestinal absorption of acylated anthocyanins in Sprague-Dawley rats, Food Chemistry, 10.1016/j.foodchem.2020.127586, 334, 127586, 2021.01, It is unknown whether intestinal absorption of acylated anthocyanins occurs in their intact or metabolized form. In this study, with the aid of matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) ima- ging, intestinal absorption of acylated anthocyanins was visually investigated. Anthocyanin extracts from purple carrots were orally administered to Sprague-Dawley rats. Acylated cyanidins were absorbed into portal and circulating blood systems in their intact form, and aglycon; cyanidin 3-O-(6-O-feruloyl-β-D-glucopyranosyl)- (1 → 6)-[β-D-xylopyranosyl-(1 → 2)]-β-D-galactopyranoside (Cy3XFGG), and showed a high absorption of 39.3 ± 0.1 pmol/mL-plasma at 60 min after administration. MALDI-MS imaging analysis of the rat jejunum membranes showed that an organic anion transporting polypeptide (OATP) transporter was involved in Cy3XFGG transport, while deacylated anthocyanins were incorporated through both the glucose transporter 2 and OATP routes. In conclusion, acylated anthocyanin, Cy3XFGG, can be absorbed in its intact form through intestinal OATP.. |
| 12. |
Baorui Li, Lei Fu, Chizumi Abe, Alexia M. Nectoux, Ayaka Yamamoto, and Toshiro Matsui, Theaflavins inhibit glucose transport across Caco-2 cells through the downregulation of the Ca2+/AMP-activated protein kinase-mediated glucose transporter SGLT1, J. Funct. Foods, https://doi.org/10.1016/j.jff.2020.104273, 75, 104273, 2020.12, The study investigated the effects of theaflavins, which are intestinally non-absorbable compounds, on intestinal
glucose transport in Caco-2 cells. 13C6-Glucose transport experiments clearly revealed that glucose transport
across Caco-2 cells was significantly (p < 0.01) inhibited by theaflavins. When Caco-2 cells were treated with 40
μM theaflavins for 24 h, the expression of SGLT1 expression was significantly (p < 0.05) suppressed, whereas no
difference in GLUT2 expression was observed. The theaflavin-induced inhibition of glucose transport was
reversed by the inhibition of influx routes mediated by OATP and MCT transporters. A Wes analysis established
that theaflavin-induced phosphorylation of AMPK was significantly (p < 0.05) suppressed by the inhibition of 2+
endoplasmic reticulum Ca -release and CaMKK β. These findings demonstrated for the first time that theaflavins can inhibit glucose transport across Caco-2 cell monolayers through the suppression of SGLT1 expression partly via the activation of the intracellular Ca2+/CaMKK β/AMPK signaling pathway.. |
| 13. |
Juneha Bak, Yoshiyuki Miyazaki, Hayato Nakano, and Toshiro Matsui, Ligand-aided 1H nuclear magnetic resonance spectroscopy for non-destructive estimation of sulfate content in sulfated saccharides, Analytical Sciences, 2020.12. |
| 14. |
Guanzhen Gao, Chizumi Abe, Alexia M. Nectoux, Shu-Wei Huang, Yuji Miyata, Kazunari Tanaka, Takashi Tanaka, Haruo Yamamura, and Toshiro Matsui, Anti-hypertensive effect of hesperidin and hesperidin-containing fermented Mikan tea in spontaneously hypertensive rats, Food Science and Technology Research, 2020.11, The anti-hypertensive effects of hesperidin and hesperidin-containing fermented Mikan tea were investigated in 8-week-old spontaneously hypertensive rats (SHRs) at a dose of 50 mg/kg body weight for both, over 20 weeks. At the end of the protocol, a significant (P < 0.05) reduction in the systolic blood pressure (SBP) of approximately 60 mmHg was observed in the SHRs with the intake of hesperidin and Mikan tea. No change in the heart rate was observed between groups. Hesperidin altered the impaired vasomotor response in 28-week-old SHRs. Investigation of receptor expression related to mitogen-stimulated vasomotor action revealed that the Mas receptor (MasR) in the aorta of hesperidin-administered SHRs was up-regulated, while no changes in angiotensin II-type 1 and type 2 receptor expressions were observed. An increase in cAMP levels was confirmed in the aorta of hesperidin-administered SHRs, demonstrating that anti-hypertensive hesperidin plays a role in vessel regulation via the upregulated-MasR/cAMP axis.. |
| 15. |
Hiroyuki Kubota, Koji Sakamoto, and Toshiro Matsui, A confocal Raman microscopic visualization of small penetrants in cellulose acetate using a deuterium-labeling technique, Scientific Reports, https://doi.org/10.1038/s41598-020-73464-8, 10, 16426, 2020.10. |
| 16. |
Akira Tsujita, Asami Nagasaka, Hidehiko Okazaki, Shin Ogawa, Akinaga Gohda, and Toshiro Matsui, Quantitative determination of H2 in human blood by 22Ne-aided gas chromatography-mass spectrometry using a single quadrupole instrument, Analytical Sciences, 2020.10. |
| 17. |
Mitsuru Tanaka, Chung Hsuan, Masataka Oeki, Weilin Shen, Asuka Goda, Yusuke Tahara, Takeshi Onodera, Keisuke Sanematsu, Tomotsugu Rikitake, Eiji Oki, Yuzo Ninomiya, Rintaro Kurebayashi, Hideto Sonoda, Yoshihiko Maehara, Kiyoshi Toko, Toshiro Matsui, Identification of characteristic compounds of moderate volatility in breast cancer cell lines, PloS one, 10.1371/journal.pone.0235442, 15, 6, e0235442, 2020.06, In this study, we were challenging to identify characteristic compounds in breast cancer cell lines. GC analysis of extracts from the culture media of breast cancer cell lines (MCF-7, SK-BR-3, and YMB-1) using a solid-phase Porapak Q extraction revealed that two compounds of moderate volatility, 1-hexadecanol and 5-(Z)-dodecenoic acid, were detected with markedly higher amount than those in the medium of fibroblast cell line (KMST-6). Furthermore, LC-TOF/MS analysis of the extracts clarified that in addition to the above two fatty acids, the amounts of five unsaturated fatty acids [decenoic acid (C10:1), decadienoic acid (C10:2), 5-(Z)-dodecenoic acid (C12:1), 5-(Z)-tetradecenoic acid (C14:1), and tetradecadienoic acid (C14:2)] in MCF-7 medium were higher than those in medium of KMST-6. Interestingly, H2O2-oxidation of 5-(Z)-dodecenoic acid and 5-(Z)-tetradecenoic acid produced volatile aldehydes that were reported as specific volatiles in breath from various cancer patients, such as heptanal, octanal, nonanal, decanal, 2-(E)-nonenal, and 2-(E)-octenal. Thus, we concluded that these identified compounds over-produced in breast cancer cells in this study could serve as potential precursors producing reported cancer-specific volatiles.. |
| 18. |
Ye Zhang, Kazuhiro Takao, Chizuru Abe, Kuni Sasaki, Koji Ochiai, and Toshiro Matsui, Intestinal absorption of prenylated isoflavones, glyceollins, in Sprague-Dawley rats., Journal of Agricultural and Food Chemistry, 10.1021/acs.jafc.0c02475, 68, 31, 8205-8211, 2020.06. |
| 19. |
Takashi Ichinose, Hiroyasu Murasawa, Tomoko Ishijima, Shinji Okada, Keiko Abe, Saki Matsumoto, Toshiro Matsui, Shigeki Furuya, Tyr-Trp administration facilitates brain norepinephrine metabolism and ameliorates a short-term memory deficit in a mouse model of Alzheimer’s disease, PloS one, 10.1371/journal.pone.0232233, 15, 5, e0232233, 2020.05, The physiological actions of orally ingested peptides on the brain remain poorly understood. This study examined the effects of 39 orally administered synthetic Tyr-containing dipeptides on the enhancement of brain norepinephrine metabolism in mice by comparing the concentration of 3-methoxy-4-hydroxyphenylethyleneglycol (MHPG). Although Tyr-Tyr administration increased blood and cerebral cortex (Cx) Tyr concentrations the most, Tyr-Trp increased Cx MHPG concentration the most. The oral administration of Tyr-Trp ameliorated a short-term memory deficit of a mouse model of cognitive dysfunction induced by amyloid beta peptide 25–35. Gene expression profiling of mouse brain using a microarray indicated that Tyr-Trp administration led to a wide variety of changes in mRNA levels, including the upregulation of genes encoding molecules involved in catecholamine metabolism. A comparative metabolome analysis of the Cx of mice given Tyr-Trp or Tyr-Tyr demonstrated that Tyr-Trp administration yielded higher concentrations of Trp and kynurenine pathway metabolites than Tyr-Tyr administration, as well as higher L-dopa levels, which is the initial product of catecholamine metabolism. Catecholamines were not significantly increased in the Cx of the Tyr-Tyr group compared with the Tyr-Trp group, despite a marked increase in Tyr. Presumably, Tyr-Trp administration enhances catecholamine synthesis and metabolism via the upregulation of genes involved in Tyr and Trp metabolism as well as metabolites of Tyr and Trp. These findings strongly suggest that orally ingested Tyr-Trp modulates the brain metabolome involved in catecholamine metabolism and contributes to higher brain function.. |
| 20. |
Mitsuru Tanaka, Hayato Kiyohara, Atsuko Yoshino, Akihiro Nakano, Fuyuko Takata, Shinya Dohgu, Yasufumi Kataoka, Toshiro Matsui, Brain-transportable soy dipeptide, Tyr-Pro, attenuates amyloid β peptide25-35-induced memory impairment in mice, npj Science of Food, 10.1038/s41538-020-0067-3, 4, 1, 7-10, 2020.04, In this study, experiments on amyloid β peptide25-35-induced mice were performed to provide in vivo evidence on the potential of the blood–brain barrier transportable soy dipeptide, Tyr-Pro, in combating memory impairment. We demonstrated for the first time that oral administration of Tyr-Pro (100 mg/kg, twice a day) in mice for 16 days significantly improved impaired memory by spontaneous alternation and shortened step-through latency in amyloid β-induced mice.. |
| 21. |
Kazunari Tanaka, Sakiko Yamamoto, Katsuhisa Omagari, Masahiro Yuasa, Aoi Tsuchihashi, Yuji Miyata, Yutaka Yoshino, Hiroyuki Ono, Toshiro Matsui, Haruo Yamamura, Effects of Feeding Hesperidin Derived from Fermented Tea Leaves Made by Tea-rolling Processing of Thinned Satsuma Mandarin Fruit and Green Tea Leaves on Blood Pressure-Randomized, Double-blind, Placebo-controlled, Parallel-group Comparative Study, Japanese Pharmacology and Therapeutics, 48, 2, 225-235, 2020.02, Objectives We developed a fermented tea made by mixing thinned satsuma Mandarin fruit and green tea leaves at the level of 1 =3. This fermented tea contained hesperidin which has the high water-soluble property. We investigated the effects of feeding hesperidin derived from fer-mented tea leaves for 12 weeks on blood pressure in volunteers. Methods Thirty nine Subjects with high-normal blood pressure and stage I hypertension participated in a randomized, double-blind, placebo-controlled, parallel-group comparative test. Subjects consumed test beverage containing 1.20g fermented tea leaves or placebo beverAâge without tea leaves for 12 weeks. Systolic and diastolic blood pressures were measured every 4 weeks. Results Beverage containing fermented tea leaves reduced systolic blood pressures after the start of feeding. Also, systolic blood pressures were significantly lower in the test beverage group than in the placebo beverage group during feeding period. Conclusions The results suggested that feeding hesperidin derived from fermented lea leaves made by mixing thinned satsuma Mandarin fruit and tea leaves has an effect decreasing systolic blood pressures in subjects with high-nomial blood pressure and stage I hypertension.. |
| 22. |
Hsuan Chung, Satoshi Tajiri, Mai Hyoguchi, Riho Koyanagi, Akihiro Shimura, Fuyuko Takata, Shinya Dohgu, Toshiro Matsui, Analysis of catecholamine and their metabolites in mice brain by liquid chromatography-mass spectrometry using sulfonated mixed-mode copolymer column, analytical sciences, 10.2116/analsci.18P494, 35, 4, 433-439, 2019.01, In this study, a simultaneous assay for catecholamines and their metabolites in the brain was established using liquid chromatography-mass spectrometry (LC-MS). To achieve complete separation, a cation-exchange/reversed-phase mixedmode copolymer resin column containing 0.81 wt% sulfo groups was used for the simultaneous LC-MS assay. The analyzed catecholamines were dopamine (DA), norepinephrine (NE), and epinephrine (E), while the metabolites lacking amino groups were 3,4-dihydroxyphenylacetic acid (DOPAC), homovanillic acid (HVA), and 3-methoxy-4- hydroxyphenylglycol (MHPG). The metabolites were separated and detected using LC-MS, on columns with and without sulfo groups. However, we could not achieve adequate separation of catecholamines on both columns using a gradient elution of 0 - 50 (v/v)% methanol containing 0.1 (v/v)% formic acid (FA). When volatile ion-pairing reagents were added to the mobile phase, they improved the retention and detection of catecholamines on the sulfonated mixed-mode column. Under optimized elution conditions, which involved a linear gradient elution of water containing 0.1 (v/v)% FA to 50 (v/v)% acetonitrile in 50 mM ammonium formate at 40°C and a 0.20 mL/min rate, all six target molecules were simultaneously detected within 25 min, when using negative mode LC-MS on a sulfonated mixed-mode column. The limits of detection (LODs) for DA, NE, E, DOPCA, HVA, and MHPG were determined to be 20.7, 12.6, 74.6, 1110, 18.7, and 3196 nM, respectively. Moreover, the established LC-MS assay allowed the detection of endogenous DA, NE, and HVA, in normal mouse brain samples at concentrations higher than 20, 9, and 4 pmol/mg, respectively.. |
| 23. |
Alexia M. Nectoux, Chizumi Abe, Shu Wei Huang, Naoto Ohno, Junji Tabata, Yuji Miyata, Kazunari Tanaka, Takashi Tanaka, Haruo Yamamura, Toshiro Matsui, Absorption and Metabolic Behavior of Hesperidin (Rutinosylated Hesperetin) after Single Oral Administration to Sprague-Dawley Rats, Journal of Agricultural and Food Chemistry, 10.1021/acs.jafc.9b03594, 67, 35, 9812-9819, 2019.09, We investigated the absorption and metabolic behavior of hesperidin (hesperetin-7-O-rutinoside) in the blood system of Sprague-Dawley rats by liquid chromatography- and matrix-assisted laser desorption ionization mass spectrometries (LC-MS and MALDI-MS). After a single oral administration of hesperidin (10 mg/kg), which was expected to be absorbed in its degraded hesperetin form, we detected intact hesperidin in the portal vein blood (tmax, 2 h) for the first time. We successfully detected glucuronized hesperidin in the circulating bloodstream, while intact hesperidin had disappeared. Further MS analyses revealed that homoeriodictyol and eriodictyol conjugates were detected in both portal and circulating blood systems. This indicated that hesperidin and/or hesperetin are susceptible to methylation and demethylation during the intestinal membrane transport process. Sulfated and glucuronized metabolites were also detected in both blood systems. In conclusion, hesperidin can enter into the circulating bloodstream in its conjugated forms, together with the conjugated forms of hesperetin, homoeriodictyol, and/or eriodictyol.. |
| 24. |
Huu Nghi Nguyen, Mitsuru Tanaka, Baorui Li, Tomoya Ueno, Hideki Matsuda, Toshiro Matsui, Novel in situ visualisation of rat intestinal absorption of polyphenols via matrix-assisted laser desorption/ionisation mass spectrometry imaging, Scientific reports, 10.1038/s41598-019-39405-w, 9, 1, 2019.12, Matrix-assisted laser desorption/ionisation mass spectrometry imaging (MALDI-MSI) is presently used in physiological evaluations for visualisation of targets in organs. In the present study, MALDI-MSI was used as a visualisation technique to investigate the intestinal absorption of polyphenols. Nifedipine/phytic acid-aided MALDI-MSI was performed to visualise theaflavin-3′-O-gallate (TF3′G) and epicatechin-3-O-gallate (ECG) in the rat jejunum for 50-µM, 60-min transport experiments. Non-absorbable TF3′G was successfully visualised at the apical region, whereas absorbable ECG was detected throughout the rat jejunum. MALDI-MSI was also performed to determine the transport routes of the target metabolites. Signals corresponding to TF3′G and ECG in the membranes were diminished following treatment with inhibitors targeting the monocarboxylic acid transporter and organic anion transporting polypeptides. Enhanced visualisation of TF3′G was achieved by inhibiting efflux routes. Our findings demonstrated that the present MALDI-MSI can provide critical spatial informations on intestinal absorption of targets, by which TF3′G and ECG were incorporated into intestinal tissues, followed by efflux back to the apical compartment. In addition, MALDI-MSI analyses suggested that TF3′G was resistant to phase II metabolism during the influx/efflux processes, whereas ECG was susceptible to methylation and sulphation reactions. In conclusion, inhibitor-aided MALDI-MSI could serve as a powerful in situ visualisation technique for verifying intestinal transport routes and investigating the metabolism of penetrants.. |
| 25. |
Mitsuru Tanaka, Shinya Dohgu, Genki Komabayashi, Hayato Kiyohara, Fuyuko Takata, Yasufumi Kataoka, Takashi Nirasawa, Motohiro Maebuchi, Toshiro Matsui, Brain-transportable dipeptides across the blood-brain barrier in mice, Scientific reports, 10.1038/s41598-019-42099-9, 9, 1, 2019.12, Apart from nutrients required for the brain, there has been no report that naturally occurring peptides can cross the blood-brain barrier (BBB). The aim of this study was to identify the BBB-transportable peptides using in situ mouse perfusion experiments. Based on the structural features of Gly-N-methylated Gly (Gly-Sar), a reported BBB-transportable compound, 18 dipeptides were synthesized, and were perfused in the mouse brain for two minutes. Among the synthesized dipeptides, Gly-Sar, Gly-Pro, and Tyr-Pro were transported across the BBB with K
i
values of 7.60 ± 1.29, 3.49 ± 0.66, and 3.53 ± 0.74 µL/g·min, respectively, and accumulated in the mouse brain parenchyma. Additionally, using MALDI-MS/MS imaging analysis of Tyr-Pro-perfused brain, we provide evidence for Tyr-Pro accumulation in the hippocampus, hypothalamus, striatum, cerebral cortex, and cerebellum of mouse brain.. |
| 26. |
Anti-diabetic Effect of Acetic Acid-Free Red Vinegar in Spontaneously Diabetic Torii Rats. |
| 27. |
Tatsuro Yamamoto, Chiyomi Sakamoto, Hiroaki Tachiwana, Mitsuru Kumabe, Toshiro Matsui, Tadatoshi Yamashita, Masatoshi Shinagawa, Koji Ochiai, Noriko Saitoh, Mitsuyoshi Nakao, Endocrine therapy-resistant breast cancer model cells are inhibited by soybean glyceollin I through Eleanor non-coding RNA, Scientific reports, 10.1038/s41598-018-33227-y, 8, 15202, 2018.10, Long-term estrogen deprivation (LTED) of an estrogen receptor (ER) α-positive breast cancer cell line recapitulates cancer cells that have acquired estrogen-independent cell proliferation and endocrine therapy resistance. Previously, we have shown that a cluster of non-coding RNAs, Eleanors (ESR1 locus enhancing and activating non-coding RNAs) formed RNA cloud and upregulated the ESR1 gene in the nuclei of LTED cells. Eleanors were inhibited by resveratrol through ER. Here we prepared another polyphenol, glyceollin I from stressed soybeans, and identified it as a major inhibitor of the Eleanor RNA cloud and ESR1 mRNA transcription. The inhibition was independent of ER, unlike one by resveratrol. This was consistent with a distinct tertiary structure of glyceollin I for ER binding. Glyceollin I preferentially inhibited the growth of LTED cells and induced apoptosis. Our results suggest that glyceollin I has a novel role in LTED cell inhibition through Eleanors. In other words, LTED cells or endocrine therapy-resistant breast cancer cells may be ready for apoptosis, which can be triggered with polyphenols both in ER-dependent and ER-independent manners.. |
| 28. |
Ayano Shimizu, Takakazu Mitani, Sachi Tanaka, Hiroshi Fujii, Motohiro Maebuchi, Yusuke Amiya, Mitsuru Tanaka, Toshiro Matsui, Soichiro Nakamura, Shigeru Katayama, Soybean-Derived Glycine-Arginine Dipeptide Administration Promotes Neurotrophic Factor Expression in the Mouse Brain, Journal of Agricultural and Food Chemistry, 10.1021/acs.jafc.8b01581, 66, 30, 7935-7941, 2018.08, Brain-derived neurotrophic factor (BDNF), a member of the neurotrophin family, plays an important role in cognitive abilities, including memory and learning. We demonstrated that soybean protein hydrolysate (SPH) diet suppresses age-related cognitive decline via the upregulation of BDNF in a mouse model of senescence. Our purpose was to identify novel bioactive peptides in SPH, which enhance BDNF expression. We treated mouse primary astrocytes with SPH as well as with its positively charged chromatographic fraction. Significant increases in the expression of BDNF were observed in the treatment with positively charged fraction of SPH. Among the synthesized peptides, the dipeptide glycine-arginine (GR) increased BDNF expression in vitro, and LC-TOF-MS analysis showed the presence of GR in the SPH. Furthermore, its administration in vivo increased the expression of BDNF in the cerebral cortex and the number of neurons in hippocampus and cerebral cortex. These data indicate that GR might promote neurogenesis by upregulating BDNF levels.. |
| 29. |
Hsuan Chung, Akihiro Shimura, Toshiro Matsui, Discriminant and simultaneous HPLC analysis of reducing and nonreducing monosaccharides on a polyethyleneimine-attached hydrophilic interaction liquid chromatography column, Food Science and Technology Research, 10.3136/fstr.24.501, 24, 3, 501-508, 2018.06, The aim of this study was to develop an analytical method for the discrimination of reducing and non-reducing monosaccharides including rare sugars on a prototype hydrophilic interaction liquid chromatography (HILIC) column. The HILIC column was composed of a glycidyl methacrylate-ethylene glycol dimethacrylate copolymer attached to 8 wt% polyethyleneimine. Seven monosaccharides (reducing aldoses: D-xylose, D-allose, and D-glucose; non-reducing ketoses: D-fructose, D-psicose, D-sorbose, and D-tagatose) were targeted. In individual HPLC analysis of both aldoses and ketoses, elution with 90 v/v% acetonitrile permitted successful detection and separation only for the non-reducing ketoses. Besides, elution of a mixture of ketoses and aldoses with 85 v/v% acetonitrile containing 5 mmol/L sodium 1-octanesulfonate (OS) (pH 4.8) enabled the simultaneous separation of all seven monosaccharides within 40 min. The polyethyleneimine-attached HILIC column allowed discriminant HPLC analysis for non-reducing ketoses using elution with 90 v/v% acetonitrile, whereas elution with 85 v/v% acetonitrile containing 5 mmol/L OS simultaneously detected both ketoses and aldoses.. |
| 30. |
Ryoko Yamamoto, Hisanori Minami, Hiromi Matsusaki, Mami Sakashita, Naoki Morita, Osamu Nishimiya, Naonobu Tsutsumi, Masashi Hosokawa, Yutaka Itabashi, Toshiro Matsui, Kazuhiro Ura, Consumption of the edible sea urchin Mesocentrotus nudus attenuates body weight gain and hepatic lipid accumulation in mice, Journal of Functional Foods, 10.1016/j.jff.2018.04.063, 47, 40-47, 2018.04, The beneficial health effects of edible sea urchin consumption in mice fed a normal (ND) or high-fat diet (HFD) were investigated in this study. Notably, sea urchin-administered (250, 500, 1000 mg/kg) mice exhibited lower body, liver, and visceral fat weights, lower plasma levels of aspartate aminotransferase and alanine aminotransferase, and lower hepatic triacylglycerol levels than those fed carboxymethyl cellulose (CMC). Despite the high levels of cholesterol found in sea urchins, intake of these organisms had no effect on plasma cholesterol levels among the mice tested. Additionally, sea urchin consumption resulted in enhanced levels of arachidonic acid and docosahexaenoic acid within mouse livers. Lastly, mice fed HFD with sea urchin (500 mg/kg) exhibited increased mRNA expression of uncoupling protein-1 within brown adipose tissue, compared with those fed HFD with CMC. In conclusion, consumption of sea urchin might provide a protective effect against the development of obesity and/or nonalcoholic fatty liver disease.. |
| 31. |
Baorui Li, Yusuke Terazono, Naoto Hirasaki, Yuki Tatemichi, Emiko Kinoshita, Akio Obata, Toshiro Matsui, Inhibition of Glucose Transport by Tomatoside A, a Tomato Seed Steroidal Saponin, through the Suppression of GLUT2 Expression in Caco-2 Cells, Journal of Agricultural and Food Chemistry, 10.1021/acs.jafc.7b06078, 66, 6, 1428-1434, 2018.02, We investigated whether tomatoside A (5α-furostane-3β,22,26-triol-3-[O-β-d-glucopyranosyl (1→2)-β-d-glucopyranosyl (1→4)-β-d-galactopyranoside] 26-O-β-d-glucopyranoside), a tomato seed saponin, may play a role in the regulation of intestinal glucose transport in human intestinal Caco-2 cells. Tomatoside A could not penetrate through Caco-2 cell monolayers, as observed in the transport experiments using liquid chromatography-mass spectrometry. The treatment of cells with 10 μM tomatoside A for 3 h resulted in a 46.0% reduction in glucose transport as compared to untreated cells. Western blotting analyses revealed that tomatoside A significantly (p < 0.05) suppressed the expression of glucose transporter 2 (GLUT2) in Caco-2 cells, while no change in the expression of sodium-dependent glucose transporter 1 was observed. In glucose transport experiments, the reduced glucose transport by tomatoside A was ameliorated by a protein kinase C (PKC) inhibitor and a multidrug resistance-associated protein 2 (MRP2) inhibitor. The tomatoside A-induced reduction in glucose transport was restored in cells treated with apical sodium-dependent bile acid transporter (ASBT) siRNA or an ASBT antagonist. These findings demonstrated for the first time that the nontransportable tomato seed steroidal saponin, tomatoside A, suppressed GLUT2 expression via PKC signaling pathway during the ASBT-influx/MRP2-efflux process in Caco-2 cells.. |
| 32. |
Si Jing Chen, Chiwa Aikawa, Risa Yoshida, Tomoaki Kawaguchi, Toshiro Matsui, Anti-prediabetic effect of rose hip (Rosa canina) extract in spontaneously diabetic Torii rats, Journal of the Science of Food and Agriculture, 10.1002/jsfa.8254, 97, 12, 3923-3928, 2017.09, BACKGROUND: Prediabetes, a high-risk state for developing diabetes showing impaired glucose tolerance but a normal fasting blood glucose level, has an increasing prevalence worldwide. However, no study investigating the prevention of impaired glucose tolerance at the prediabetic stage by anti-diabetic functional foods has been reported. Thus, the present study aimed to evaluate the anti-prediabetic effect of rose hip in a prediabetic rat model. RESULTS: Spontaneously diabetic Torii (SDT) rats were supplemented with hot-water extract of rose hip at a dose of 100 mg kg–1 body weight day–1 for 12 weeks. The results obtained showed that the supplementation of rose hip extract improved impaired glucose tolerance, promoted insulin secretion, preserved pancreatic beta-cell function and suppressed plasma advanced glycation end-products formation of methylglyoxal-derived hydroimidazolone (MG-H1) residue and Nϵ-carboxymethyl-lysine residues (e.g. MG-H1, control: 465.5 ± 43.8 versus rose hip: 59.1 ± 13.0 pmol mg protein–1, P < 0.05) in SDT rats at the prediabetic stage (12–20 weeks old). CONCLUSION: The present study provides the first evidence showing that a hot-water extract of rose hip could exert an anti-prediabetic effect in a rat model.. |
| 33. |
Vu Thi Hanh, Weilin Shen, Mitsuru Tanaka, Aino Siltari, Riita Korpela, Toshiro Matsui, Effect of Aging on the Absorption of Small Peptides in Spontaneously Hypertensive Rats, Journal of Agricultural and Food Chemistry, 10.1021/acs.jafc.7b01727, 65, 29, 5935-5943, 2017.07, In the present study, we aimed to evaluate the effect of aging on the absorption of small peptides in spontaneously hypertensive rats (SHRs). Three kinds of dipeptides, glycyl-sarcosine (Gly-Sar), Trp-His, and captopril (a dipeptidomimetic drug), a Gly-Sar-Sar tripeptide, a Gly-Sar-Sar-Sar tetrapeptide, and a Gly-Sar-Sar-Sar-Sar pentapeptide were administered at doses of 10 mg/kg each to 8- and 40-week-old SHRs. The peptides were all detected in their intact forms in the blood. There was a significantly promoted absorption of di/tripeptides in aged SHRs compared with young SHRs. In contrast, the absorption of tetra/pentapeptides was not affected by aging. PepT1 expression in the mid-jejunum was significantly increased in 40-week-old SHRs compared with 8-week-old SHRs, whereas aging did not alter the expression of claudin-1, a tight junction related protein. Thus, the present results suggest that SHR aging may enhance the absorption of di/tripeptides through the enhanced PepT1 transport route, although oligopeptides may be absorbed in an age-independent manner.. |
| 34. |
Gonzalo Miyagusuku-Cruzado, Naoki Morishita, Keiichi Fukui, Norihiko Terahara, Toshiro Matsui, Anti-prediabetic effect of 6-O-caffeoylsophorose in prediabetic rats and its stimulation of glucose uptake in L6 Myotubes, Food Sci. Technol. Res., 23, 3, 449-456, 2017.06. |
| 35. |
Thanutchaporn Kumrungsee, Sayaka Akiyama, Jian Guo, Mitsuru Tanaka, Matsui Toshiro, Identification of peptides in wheat germ hydrolysate that demonstrate calmodulin-dependent protein kinase II inhibitory activity, FOOD CHEMISTRY, 10.1016/j.foodchem.2016.06.059, 213, 329-335, 2016.12. |
| 36. |
THuu-Nghi Nguyen, Mitsuru Tanaka, Genki Komabayashi, Matsui Toshiro, The photobase generator nifedipine as a novel matrix for the detection of polyphenols in matrix-assisted laser desorption/ionization mass spectrometry, JOURNAL OF MASS SPECTROMETRY, 10.1002/jms.3805, 51, 10, 748-756, 2016.10. |
| 37. |
Toshihiko Fukuda, Takanori Kuroda, Miki Kono, Mai Hyoguchi, Satoshi Tajiri, Mitsuru Tanaka, Yoshinori Mine, Matsui Toshiro, Adenine attenuates the Ca2+ contraction-signaling pathway via adenine receptor-mediated signaling in rat vascular smooth muscle cells, NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY, 10.1007/s00210-016-1264-0, 389, 9, 999-1007, 2016.09. |
| 38. |
Thanutchaporn Kumrungsee, Sayaka Akiyama, Tomomi Saiki, Mitsuru Tanaka, Masao Omae, Kazuhiro Hamasawa, Matsui Toshiro, Vasorelaxation effect of 5’-methylthioadenosine obtained from Candida utilis yeast extract though the suppression of intracellular Ca2+ concentration in rat isolated aorta, JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 64, 17, 3362-3370, 2016.07. |
| 39. |
Akiko Mizokami, DaGuang Wang, Genki Komabayashi, Mitsuru Tanaka, Jing Gao, Hiroshi Takeuchi, Matsui Toshiro, Hirata Masato, An extract from pork bones containing osteocalcin improves glucose metabolism in mice by oral administration., Bioscience, Biotechnology, and Biochemistry, 10.1080/09168451.2016.1214530, 80, 11, 2176-2183, 2016.07. |
| 40. |
Toshihiko Fukuda, Kaustav Majumder, Hua Zhang, Patricia V Turner, Matsui Toshiro, Yoshinori Mine, Adenine Inhibits TNF-alpha Signaling in Intestinal Epithelial Cells and Reduces Mucosal Inflammation in a Dextran Sodium Sulfate-Induced Colitis Mouse Model, JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 10.1021/acs.jafc.6b00665, 64, 21, 4227-4234, 2016.06. |
| 41. |
Jian Guo, Naoto Hirasaki, Yuji Miyata, Kazunari Tanaka, TakashiTanaka, Xiao Wu, Yusuke Tahara, Kiyoshi Toko, Matsui Toshiro, Evaluating the Reduced Hydrophobic Taste Sensor Response of Dipeptides by Theasinensin A by Using NMR and Quantum Mechanical Analyses, PLOS ONE, 10.1371/journal.pone.0157315, 11, 6, e0157315, 2016.06, 重合カテキンであるテアシネンシンAが苦みペプチドである各種のトリプトファン含有ジペプチドの苦み応答を抑制し、かつ園現象は溶液内での両者の複合体形成であることをin vitroおよびin silico解析によって突き止めたこと。. |
| 42. |
Hirosuke Inoue, Hisanori Nishio, Hidetoshi Takada, Etsuko Nanishi, Mitsuho Onimaru, Si Jing Chen, Matsui Toshiro, Toshiro Hara, Activation of Nod1 signaling induces fetal growth restriction and death through fetal and maternal vasculopathy, Journal of Immunology, 196, 2779-2787, 2016.05. |
| 43. |
Seong-Min Hong, Mitsuru Tanaka, Riho Koyanagi, Weilin Shen, Matsui Toshiro, Structural Design of Oligopeptides for Intestinal Transport Model, JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 10.1021/acs.jafc.6b00279, 64, 10, 2072-2079, 2016.03. |
| 44. |
Vu Thi Hanh, Yutaro Kobayashi, Motohiro Maebuchi, Toshihiro Nakamori, Mitsuru Tanaka, Matsui Toshiro, Quantitative mass spectrometric analysis of dipeptides in protein hydrolysate by a TNBS derivatization-aided standard addition method, FOOD CHEMISTRY, 10.1016/j.foodchem.2015.05.053, 190, 345-350, 2016.01. |
| 45. |
Yusuke Ushida, Boonyapichest Chutinan, Hiroyuki Suganuma, Mitsuru Tanaka, Matsui Toshiro, Paracellular Transport of Sulforaphane across Caco-2 Cell Monolayers, FOOD SCIENCE AND TECHNOLOGY RESEARCH, 10.3136/fstr.22.127, 22, 1, 127-134, 2016.01. |
| 46. |
Jian Zhao, Aki Suyama, Hsuan Chung, Toshihiko Fukuda, Mitsuru Tanaka, Matsui Toshiro, Ferulic acid enhances nitric oxide production through up-regulation of argininosuccinate synthase in inflammatory human endothelial cells, LIFE SCIENCES, 10.1016/j.lfs.2015.12.044, 145, 224-232, 2016.01. |
| 47. |
Toshihiko Fukuda, Takanori Kuroda, Miki Kono, Mai Hyoguchi, Mitsuru Tanaka, Matsui Toshiro, Augmentation of ferulic acid-induced vasorelaxation with aging and its structure importance in thoracic aorta of spontaneously hypertensive rats, NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY, 10.1007/s00210-015-1171-9, 388, 10, 1113-1117, 2015.10. |
| 48. |
Takashi Ichinose, Kazuki Moriyasu, Akane Nakahara, Mitsuru Tanaka, Matsui Toshiro, Shigeki Furuya, Orally administrated dipeptide Ser-Tyr efficiently stimulates noradrenergic turnover in the mouse brain, BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 10.1080/09168451.2015.1044932, 79, 9, 1542-1547, 2015.09. |
| 49. |
Mitsuru Tanaka, Seong-Min Hong, Sayaka Akiyama, Qing-Qiang Hu, Matsui Toshiro, Visualized absorption of anti-atherosclerotic dipeptide, Trp-His, in Sprague-Dawley rats by LC-MS and MALDI-MS imaging analyses, MOLECULAR NUTRITION & FOOD RESEARCH, 10.1002/mnfr.201500075, 59, 8, 1541-1549, 2015.08. |
| 50. |
Ruge Cao, Yutaro Kobayashi, Airi Nonaka, Yuji Miyata, Kazunari Tanaka, Takashi Tanaka, Matsui Toshiro, NMR Spectroscopic and Quantum Mechanical Analyses of Enhanced Solubilization of Hesperidin by Theasinensin A, PHARMACEUTICAL RESEARCH, 10.1007/s11095-015-1621-6, 32, 7, 2301-2309, 2015.07. |
| 51. |
Si Jing Chen, Chiwa Aikawa, Risa Yoshida, Matsui Toshiro, Methylglyoxal-derived hydroimidazolone residue of plasma protein can behave as a predictor of pre-diabetes in Spontaneously Diabetic Torii rats, Physiological Reports, doi10.14814/phy2.12477, 3, 7, e12477, 2015.07. |
| 52. |
Ruge Cao, Airi Nonaka, Fusae Komura, Matsui Toshiro, Application of diffusion ordered-H-1-nuclear magnetic resonance spectroscopy to quantify sucrose in beverages, FOOD CHEMISTRY, 10.1016/j.foodchem.2014.08.105, 171, 8-12, 2015.03. |
| 53. |
Si Jing Chen, Chiwa Aikawa, Matsui Toshiro, Qantitative analysis of methylglyoxal, glyoxal and free advanced glycation end-products in plasma of Wistar rats during oral glucose tolerance test, Biological Pharmacology Bulletin, 38, 2, 336-339, 2015.02. |
| 54. |
Ha-Young Park, Yuri Kunitake, Naoto Hirasaki, Mitsuru Tanaka, Matsui Toshiro, Theaflavins enhance intestinal barrier of Caco-2 Cell monolayers through the expression of AMP-activated protein kinase-mediated Occludin, Claudin-1, and ZO-1, BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 10.1080/09168451.2014.951027, 79, 1, 130-137, 2015.01. |
| 55. |
Jiang Guo, Tomomi Saiki, Kumrungsee Thanutchaporn, Wanying Liu, Akihiro Shimura, Matsui Toshiro, Elution Profile of Di-peptides on a Sulfonated Ethylstyrene-Divinylbenzene Copolymer Resin Column by High-performance Liquid Chromatography, ANALYTICAL SCIENCES, 31, 1, 45-50, 2015.01. |
| 56. |
Soga Minoru, Ohashi Ayaka, Taniguchi Emi, Matsui Toshiro, Tsuda Takanori, The di-peptide Trp-His activates AMP-activated protein kinase and insulin-independently enhances glucose uptake in L6 myotubes, FEBS Open Bio, 4, 898-904, 2014.11. |
| 57. |
Kumrungsee Thanutchaporn, Saiki Tomomi, Akiyama Sayaka, Nakashima Kentaro, Mitsuru Tanaka, Kobayashi Yutaro, Matsui Toshiro, Inhibition of calcium-calmodulin complex formation by vasorelaxant basic dipeptides demonstrated by in vitro and in silico analyses, BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS, 10.1016/j.bbagen.2014.07.020, 1840, 10, 3073-3078, 2014.10. |
| 58. |
Kovacs-Nolan Jennifer, Rupa Prithy, Matsui Toshiro, Mitsuru Tanaka, Konishi Toni, Sauchi Yusuke, Sato Kenji, Ono Shin, Mine Yoshinori, In Vitro and ex Vivo Uptake of Glutathione (GSH) across the Intestinal Epithelium and Fate of Oral GSH after in Vivo Supplementation, JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 10.1021/jf503257w, 62, 39, 9499-9506, 2014.10. |
| 59. |
Zhao Jian, Suyama Aki, Mitsuru Tanaka, Matsui Toshiro, Ferulic acid enhances the vasorelaxant effect of epigallocatechin gallate in tumor necrosis factor-alpha-induced inflammatory rat aorta, JOURNAL OF NUTRITIONAL BIOCHEMISTRY, 10.1016/j.jnutbio.2014.03.013, 25, 7, 807-814, 2014.07. |
| 60. |
Kumrungsee Thanutchaporn, Wang Zheng-Quan, Matsumura Shinya, Saiki Tomomi, Mitsuru Tanaka, Matsui Toshiro, Identification of peptides from soybean protein, glycinin, possessing suppression of intracellular Ca2+ concentration in vascular smooth muscle cells, FOOD CHEMISTRY, 10.1016/j.foodchem.2013.11.143, 152, 218-224, 2014.06. |
| 61. |
Cao Ruge, Komura Fusae, Nonaka Airi, Kato Takeshi, Fukumashi Junji, Matsui Toshiro, Quantitative analysis of D-(+)-glucose in fruit juices using diffusion ordered-1H nuclear magnetic resonance spectroscopy, Analytical Sciences, 30, 3, 383-388, 2014.03. |
| 62. |
Fukuda Toshihiko, Kuroda Takahiro, Kono Miki, Takahisa Miyamoto, Mitsuru Tanaka, Matsui Toshiro, Attenuation of L-Type Ca2+ Channel Expression and Vasomotor Response in the Aorta with Age in Both Wistar-Kyoto and Spontaneously Hypertensive Rats, PLOS ONE, 10.1371/journal.pone.0088975, 9, 2, e88975-e88983, 2014.02. |
| 63. |
Qiu Ju, Maekawa Kanako, Kitamura Yuko, Miyata Yuji, Tanaka Kazunari, Tanaka Takashi, Soga Minoru, Tsuda Takanori, Matsui Toshiro, Stimulation of glucose uptake by theasinensins through the AMP-activated protein kinase pathway in rat skeletal muscle cells, BIOCHEMICAL PHARMACOLOGY, 10.1016/j.bcp.2013.10.029, 87, 2, 344-351, 2014.01. |
| 64. |
Seong-Min Hong, Mitsuru Tanaka, Saori Yoshii, Yoshinori Mine, Matsui Toshiro, Enhanced visualization of small peptides absorbed in rat small intestine by phytic-acid-aided MALDI-imaging mass spectrometry, Anal. Chem, 85, 11, 10033-10039, 2013.11. |
| 65. |
Eduardo M. N. Nakashima, Hu-Qiang Qing, Tanaka Mitsuru, Matsui Toshiro, Improvement of liquid chromatography-tandem mass spectrometric detection of di-peptides by 2,4,6-trinitrobenzene sulfonate derivatization, Biosci. Biotechnol. Biochem, 77, 10, 2094-2099, 2013.10. |
| 66. |
Y.Miyata, S.Tamaru, K.Tamaya, Matsui Toshiro, Y.Nagata, K.Tanaka, Theflavins and theasinensin A derived from fermented tea have antihyperglycemic and hypotriacylglycerolemic effects in KK-Ay mice and Sprague-Dawley rats, J. Agric. Food Chem, 61, 10, 9366-9372, 2013.10. |
| 67. |
Takeda, Junko, Park, Ha-Young, Kunitake, Yuri, Yoshiura, Keiko, Matsui Toshiro, Theaflavins, dimeric catechins, inhibit peptide transport across Caco-2 cell monolayers via down-regulation of AMP-activated protein kinase-mediated peptide transporter PEPT1, FOOD CHEMISTRY, 10.1016/j.foodchem.2012.12.026, 138, 4, 2140-2145, 2013.06. |
| 68. |
Hoang Lan Phuong, Qiu, Ju, Matsui Toshiro, Kuwahara, Takashi, Fukui, Keiichi, Yoshiyama, Kayo, Matsugano, Kazusato, Terahara, Norihiko, Transepithelial transport of 6-O-caffeoylsophorose across Caco-2 cell monolayers, FOOD CHEMISTRY, 10.1016/j.foodchem.2012.10.137, 138, 1, 9048-9054, 2013.05. |
| 69. |
Chihiro Hashimoto, Yuri Iwaihara, Si-Jing Chen, Tanaka Mitsuru, Toshiaki Watanabe, Matsui Toshiro, Highly-Sensitive Detection of Free Advanced Glycation End-Products by Liquid Chromatography-Electrospray Ionization-Tandem Mass Spectrometry with 2,4,6-Trinitrobenzene Sulfonate Derivatization, ANALYTICAL CHEMISTRY, 10.1021/ac400294q, 85, 9, 4289-4295, 2013.05, 生体内での糖化産物である各種AGEを高感度分析する新たなアッセイ法（LC-MS/MS-MRM-TNBS法）を提案した。. |
| 70. |
Ha-Young Park, Yuri Kunitake, Matsui Toshiro, Benzotropolone moiety in theaflavins is responsible for inhibiting peptide-transport and activating AMP-activated protein kinase in Caco-2 cells., J. Functional Foods in Health and Disease, 3, 5, 111-121, 2013.05. |
| 71. |
Mahmud Tareq Hassan Khan, Kenichi Dedachi, Matsui Toshiro, Noriyuki Kurita, Monica Borgatti, Ingebrigt Sylte, Dipeptide inhibitors of thermolysin and angiotensin I-converting enzyme., Current Topics in Medicinal Chemistry, 12, 16, 1748-1762, 2012.10. |
| 72. |
Tanaka Mitsuru, Suyama Aki, J. Zhao, Matsui Toshiro, Epigallocatechin gallate enhances the endothelium-independent vasorelaxation of the anti-atherosclerotic Ca2+ channel blocking peptide Trp-His in thoracic aorta from Sprague-Dawley rats, J. Hypertens., eS 1, e279-e279, 2012.09. |
| 73. |
Toshihiko Fukuda, Yutaro kobayashi, Tanaka Mitsuru, Matsui Toshiro, Vasorelaxant di-peptide, Trp-His, regulates Ca2+-signaling pathway by inhibiting the phosphorylation of voltage-dependent L-type Ca2+ channel in vascular smooth muscle cells, J. Hypertens., eS 1, e279-e279, 2012.09, 血管弛緩ペプチドであるTrp-Hisがラット平滑筋細胞においてカルシウムシグナル系抑制を介してL型カルシウムチャンネルのリン酸化を制御していることを証明した。. |
| 74. |
Ju Qiu, Yoko Kitamura, Yuji Miyata, Shizuka Tamaru, Kazunari Tanaka, Takashi Tanaka, Matsui Toshiro, Transepithelial Transport of Theasinensins through Caco-2 Cell Monolayers and Their Absorption in Sprague-Dawley Rats after Oral Administration, J. Agric. Food Chem, 60, 8036-8043, 2012.07. |
| 75. |
Jennifer Kovacs-Nolan, Hua Zhang, Masahisa Ibuki, Toshihiro Nakamori, Keiko Yoshiura, Patricia V Turner, Matsui Toshiro, Yoshinori Mine, The PepT1-transportable soy tripeptide VPY reduces intestinal inflammation, Biochim. Biophys. Acta-General Subjects, 1820, 1753-1763, 2012.06. |
| 76. |
Eui-Baek Byun, Teruaki Ishikawa, Aki Suyama, Masaya Kono, Shohei Nakashima, Tomomasa Kanda, Miyamoto Takahisa, Matsui Toshiro, A procyanidin trimer, C1, promotes NO production in rat aortic endothelial cells via both hyperpolarization and PI3K/Akt pathways, Eur. J. Pharm, 10.1016/j.ejphar.2012.07.011, 692, 52-60, 2012.06. |
| 77. |
S.Tokuda, T.Fukuda, Y.Kobayashi, Mitsuru Tanaka, Matsui Toshiro, Uncharged imidazolium moiety in adenine is responsible for endothelium-independent relaxation in contracted thoracic aorta of Sprague-Dawley rats, Biosci. Biotechnol. Biochem., 76, 4, 828-830, 2012.04. |
| 78. |
Yutaro Kobayashi, T.Fukuda, Mitsuru Tanaka, Matsui Toshiro, The anti-atherosclerotic di-peptide, Trp-His, inhibits the phosphorylation of voltage-dependent L-type Ca2+ channels in rat vascular smooth muscle cells, FEBS Open Bio, 10.1016/j.fob.2012.04.005, 2, 83-88, 2012.04. |
| 79. |
E.B.Byun, S.Korematsu, T.Ishikawa, T.Nishizuka, S.Ohshima, Matsui Toshiro, Apple procyanidins induce hyperpolarization of rat aorta endothelial cells via activation of K+ channels, J. Nutr. Biochem., 23, 3, 278-286, 2012.03. |
| 80. |
Yuji Miyata, Takashi Tanaka, Kei Tamaya, Toshiro Matsui, Shizuka Tamaru and Kazunari Tanaka, Cholesterol-lowering effect of black tea polyphenols, theaflavins, Theasinensin A and thearubigins, in rats fed high fat diet, Food Sci. Technol. Res., 17, 6, 583-588, 2011.12. |
| 81. |
T.Shii, T.Tanaka, S.Watarumi, Y.Matsuo, Y.Miyata, K.Tamaya, S.Tamaru, K.Tanaka, T.Matsui and I.Kouno, Polyphenol composition of a functional fermented tea obtained by tea-rolling processing of green tea and loquat leaves, J. Agric. Food Chem, 59, 13, 7253-7260, 2011.07. |
| 82. |
J.Qiu, N.Saito, M.Noguchi, K.Fukui, K.Yoshiyama, K.Matsugano, N.Terahara and T.Matsui, Absorption of 6-O-caffeoylsophorose and its metabolites in Sprague-Dawley rats detected by ECD-HPLC and ESI-TOF-MS methods, J. Agric. Food Chem., 59, 11, 6299-6304, 2011.05. |
| 83. |
E. M. N. Nakashima, A.Kudo, Y. Iwaihara, M. Tanaka, K. Matsumoto, T. Matsui, Application of 13C-stable-isotope labeling LC-MRM-MS/MS method for determining intact absorption of bioactive di-peptides in rats., Anal. Biochem., 10.1016/j.ab.2011.02.037, 414, 4, 109-116, 2011.04. |
| 84. |
N. Inoue, K. Nagao, K.Sakata, N.Yamano, P.E.R.Gunawardena, S.Y.Han, T.Matsui, T.Nakamori, H.Furuta, K.Takamatsu and T.Yanagita, Screening of soy protein-derived hypotriglyceridemic di-peptides in vitro and in vivo, Lipids in Health and Disease, 10, 85-94, 2011.04. |
| 85. |
T.Hasegawa, T.Matsui, K.Matsumoto, Effect of multi stratification and film depth on visualized dynamic sorption behavior of perylene in cellulose acetate film by confocal laser scanning microscope technique., J.Appl.Polymer Sci., 121, 3, 1780-1787, 2011.03. |
| 86. |
Z.Wang, S.Watanabe, Y.Kobayashi, M.Tanaka, T.Matsui, Trp-His, a vasorelaxant di-peptide, can inhibit extracellular Ca2+ entry to rat vascular smooth muscle cells through blockade of dihydropyridine-like L-type Ca2+ channels, Peptides, 31, 2060-2066, 2010.10, 抗動脈硬化作用並びに血管弛緩作用を有するジペプチドTrp-Hisが血管平滑筋細胞膜に局在する電位依存性L型カルシウムチャンネルに結合し、降圧薬であるニフェジピンと同様に細胞内へのカルシウムイオンの流入を遮断させることを明らかにした。. |
| 87. |
T.Matsui, A.Kudo, S.Tokuda, K.Matsumoto, H.Hosoyama, Identification of a new natural vasorelaxatant compound, (+)-osbeckic acid, from rutin-free tartary buckwheat extract, J. Agric. Food Chem., 58, 20, 10876-10879, 2010.10. |
| 88. |
T.Hasegawa, T.Matsui, K.Matsumoto, Application of confocal laser scanning microscope technique to visualize dynamic sorption behavior of perylene in cellulose acetate film, J. Appl. Polymer Sci., 116, 5, 1552-1557, 2010.05. |
| 89. |
T.Hasegawa, T.Matsui, K.Matsumoto, Comparative study of additives on visualized dynamic sorption behavior of perylene in cellucose acetate film by confocal laser scanning microscope technique, J. Appl. Polymer Sci., 116, 5, 1710-1717, 2010.05. |
| 90. |
A.Toshima, T.Matsui, M.Noguchi, J.Qiu, K.Tamaya, Y.Miyata, T.Tanaka, K.Tanaka, Identification of α-glucosidase inhibitors from a new fermented tea obtained by tea-rolling processing of loquat (Eriobotrya japonica) and green tea leaves, J. Sci. Food Agr., 90, 1545-1550, 2010.05. |
| 91. |
T.Matsui, X.L.Zhu, K.Shiraishi, T.Ueki, Y.Noda, K.Matsumoto, Antihypertensive effect of salt-free soy sauce, a new fermented seasoning, in spontaneously hypertensive rats, J.Food Sci., 75, 4, H129-H134, 2010.04. |
| 92. |
T.Matsui, M.Sato, M.Tanaka, Y.Yamada, S.Watanabe, Y.Fujimoto, K.Imaizumi, K.Matsumoto, Vasodilating di-peptide Trp-His can prevent atherosclerosis in apolipoprotein E-deficient mice, Br. J. Nutr., 103, 3, 309-313, 2010.03. |
| 93. |
K.Tamaya, T.Matsui, A.Toshima, M.Noguchi, J.Qiu, Y.Miyata, T.Tanaka, K.Tanaka, Suppression of blood glucose level by a new fermented tea obtained by tea-rolling processing of loquat (Eriobotrya japonica) and green tea leaves in disaccharides-loaded Sprague-Dawley rats, J. Sci. Food Agr., 90, 779-783, 2010.03. |
| 94. |
R.Selyanchyn, S.Korposh, T.Matsui, H.Matsui, S.W.Lee, Purge and trap sampling coupled to curie point thermal desorption for the detection of parts per trillion 2,4,6-trichloroanisole in water, Chromatographia, 71, 317-321, 2010.01. |
| 95. |
Toshiro Matsui, Sachiko Korematsu, Eui-Baek Byun, Taichi Nishizuka, Shunji Ohshima and Tomomasa Kanda, Apple Procyanidins Induce Vascular Relaxation in Isolated Rat Aorta through NO/cGMP Pathway in Combination with Hyperpolarization by Multiple K+ Channel Activations, Biosci. Biotechnol. Biochem., 73(10), 2246-2251, 2009.10. |
| 96. |
Norihiko Terahara, Toshiro Matsui,Kanako Minoda, Kanae Nasu, Risa Kikuchi, Keiichi Fukui, Hiroshi Ono, and Kiyoshi Matsumoto, Functional new acylated sohoroses and deglucosylated anthocyanins in a fermented red vinegar, J. Agric. Food Chem, 57(18), 8331-8338 , 2009.10. |
| 97. |
Takashi Tanaka, Yuji Miyata, Kei Tamaya, Rie Kusano, Yusuke Matsuo, Shizuka Tamaru, Kazunari Tanaka, Toshiro Matsui, Masamichi Maeda, Isao Kouno, Increase of theaflavin gallates and thearubigins by acceleration of catechin oxidation in a new fermented tea product obtained by tea-rolling processing of loquat (Eriobotrya japonica) and green tea leaves, J. Agric. Food Chem., 57(13), 5816-5822, 2009.09. |
| 98. |
Mitsuru Tanaka, Shimpei Watanabe, Zhengquan Wang, Kiyoshi Matsumoto and Toshiro Matsui, His-Arg-Trp potently attenuates contracted tension of thoracic aorta of Sprague-Dawley rats through the suppression of extracellular Ca2+ influx, Peptides, 30, 1502-1507, 2009.06. |
| 99. |
Toshiro Matsui, Mayu Shimada, Nozomi Saitoh and Kiyoshi Matsumoto, α-Glucosidase inhibition assay in an enzyme-immobilized amino-microplate, Anal.Sci. , 25(4), 559-562 (2009), 2009.04. |
| 100. |
Masahiro Yoshiki, Keisuke Tsuge, Yumi Tsuruta, Takashi Yoshimura, Kazuyoshi Koganemaru, Toshihisa Sumi, Toshiro Matsui, Kiyoshi Matsumoto, Production of a new antioxidant compound from mycosporine-like amino acid, Porphyra-334, by heat treatment., Food Chem., 113, 1127-1132 , 2009.01. |
| 101. |
Yusuke Ushida, Toshiro Matsui, Mitsuru Tanaka, Kiyoshi Matsumoto, Hirokazu Hosoyama, Atsuhiro Mitomi, Yuko Sagesaka, Takami Kakuda, Endothelium-dependent vasorelaxation effect of rutin-free tartary buckwheat extract in isolated rat thoracic aorta. , J. Nutr. Biochem., 19(10), 700-707, 2008.10. |
| 102. |
Lieselot Vercruysse, Guy Smagghe, Toshiro Matsui, John Van Camp, Purification and Identification of an Angiotensin I-converting Enzyme (ACE) Inhibitory Peptide from the Gastrointestinal Hydrolysate of the Cotton Leafworm, Spodoptera Littoralis. , Process Biochem., 43, 900-904, 2008.10. |
| 103. |
Tomoko Fujise, Norihiko Terahara, Keiichi Fukui, Koichi Sugita, Hideaki Ohta, Toshiro Matsui, Kiyoshi Matsumoto, Durable antihyperglycemic effect of 6-O-caffeoylsophorose with α-glucosidase inhibitory activity in rats., Food Sci. Technol. Res., 14(5), 477-484, 2008.10. |
| 104. |
Mitsuru Tanaka, Shinpei Watanabe, Mai Tokuyasu, Toshiro Matsui, Kiyoshi Matsumoto, Structure-activity relationship between vasodilating di- and tri-peptides in thoracic aorta of Sprague-Dawley rats. , J. Hypertension, 26(suppl.1), S171, 2008.06. |
| 105. |
Xiao-Lin Zhu, Keisuke Watanabe, Koso Shiraishi, Tatsuro Ueki, Yoshiharu Noda, Toshiro Matsui, Kiyoshi Matsumoto, Identification of ACE-inhibitory Peptides in Salt-free Soy Sauce that are Transportable across Caco-2 Cell Monolayers, Peptides, 29(3), 338-344 , 2008.03. |
| 106. |
Xiao-Lin Zhu, Toshiro Matsui, Keisuke Watanabe, Koso Shiraishi, Tatsuro Ueki, Kiyoshi Matsumoto, Simultaneous determination of angiotensin I-converting enzyme inhibitory peptides in tryptic casein hydrolysate by high-performance liquid chromatography combined with a replicate heart-cut column-switching technique, J. Agric. Food Chem., 55(21), 8367-8374 , 2007.10. |
| 107. |
Isiaka Ajani Ogunwande, Toshiro Matsui, Tomoko Fujise and Kiyoshi Matsumoto, α-Glucosidase Inhibitory Profile of Nigerian Medicinal Plants in Immobilized Assay System, Food Sci. Technol. Res., 13(2), 169-172, 2007.05. |
| 108. |
Toshiro Matsui, Takashi Tanaka, Satomi Tamura, Asami Toshima, Kei Tamaya, Yuji Miyata, Kazunari Tanaka, and Kiyoshi Matsumoto, Alpha-Glucosidase Inhibitory Profile of Catechins and Theaflavins, J.Agric. Food Chem., 55(1), 99-105, 2007.01. |
| 109. |
Mitsuru Tanaka, Toshiro Matsui, Yusuke Ushida and Kiyoshi Matsumoto, Vasodilating action of di-peptides in thoracic aortas from spontaneously hypertensive rats, Biosci. Biotechnol. Biochem., 70(9), 2292-2295, 2006.09. |
| 110. |
Toshiro Matsui, Xiao Lin Zhu, Keisuke Watanabe, Keisuke Tanaka, Yoko Kusano, Kiyoshi Matsumoto, Combined administration of captopril with an antihypertensive Val-Tyr di-peptide to spontaneously hypertensive rats attenuates the blood pressure lowering effect, Life Sciences, 2006.08. |
| 111. |
Toshiro Matsui, Isiaka Ogunwande, Kanthi J. M. Abesundara, and Kiyoshi Matsumoto, Anti-hyperglycemic Potential of Natural Products, Mini-Reviews in Medicinal Chemistry, 6(3), 109-120, 2006.02. |
| 112. |
T.Matsui, T.Ueno, M.Tanaka, H.Oka, T.Miyamoto, K.Osajima, K.Matsumoto, Antiproliferative action of an angiotensin I-converting enzyme inhibitory peptide, Val-Tyr, via an L-type Ca2+ channel inhibition in cultured vascular smooth muscle cells, Hypertension Res., 10.1291/hypres.28.545, 28, 6, 545-552, 28(6), 545-552, 2005.06. |
| 113. |
T.Matsui, S.Ebuchi, T.Fujise, K.J.M.Abesundara, S.Doi, H.Yamada, K.Matsumoto, Strong antihyperglycemic effects of water-soluble fraction of Brazilian propolis and its bioactive constituent, 3, 4, 5-tri-O-caffeoylquinic acid, Biol. Pharm. Bull., 10.1248/bpb.27.1797, 27, 11, 1797-1803, 27, 1797-1803, 2004.11. |
| 114. |
K.Abesundara, T.Matsui, K.Matsumoto, a-Glucosidase inhibitory activity of some Sri Lanka plant extracts, One of which, Cassia auriculata, exerts a strong antihyperglycemic effect in rats comparable to the therapeutic drug acarbose, J.Agric. Food Chem., 10.1021/jf035330s, 52, 9, 2541-2545, 52, 2541-2544, 2004.05. |
| 115. |
T.Matsui, M.Imamura, H.Oka, K.Osajima, K.Kimoto, T.Kawasaki, K.Matsumoto, Tissue distribution of antihypertensive dipeptide, Val-Tyr, after its single oral administration to spontaneously hypertensive rats, J.Peptide Sci., 10.1002/psc.568, 10, 9, 535-545, 10,535-545, 2004.01. |
| 116. |
H.Oka, T.Matsui, T.Ueno, K.Matsumoto, T.Kawasaki, ACE inhibitory peptides can act as an inhibitor of vascular smooth muscle cell growth, J.Hypertension, 22, S68-S68, 22(suppl 1), S68, 2004.01. |
| 117. |
T.Matsui, T.Kawasaki, Classification of natural ACE inhibitory peptides into C-/N-domain selective inhibitor, J.Hypertension, 22, S70-S70, 22(suppl. 1), S70, 2004.01. |
| 118. |
Physiological function and preparation of antihypertensive peptide, SVY, from 7S globulin.. |
| 119. |
Antihypertensive effect of food containing sardine peptides in high-normal hypertensives amd hypertensives.. |
| 120. |
SOD-like activity of sardine muscle hydrolysate by alkaline protease.. |
| 121. |
Intestinal absorption behavior of bioactive peptides.. |
| 122. |
K.Nagao, N.Inoue, Y.-M.Wang. J.Hirata, Y.Shimada, T.Nagao, T.Matsui, and T.Yanagita, The 10trans,12cis isomer of conjugated linoleic acid suppress the development of hypertension in Otsuka Long-Evans Tokushima fatty rats, Biochem. Biophys. Res. Commun., 10.1016/S0006-291X(03)00929-X, 306, 1, 134-138, 306, 134-138, 2003.05. |
| 123. |
T.Matsui, A.Hayashi, K.Tamaya, K.Matsumoto, T.Kawasaki, K.Murakami, and K.Kimoto, Depressor effect induced by dipeptide, Val-Tyr, in hypertensive transgenic mice is due, in part, to the suppression of human circulating renin-angiotensin system, Clin. Exp. Pharmacol. Physiol., 10.1046/j.1440-1681.2003.03824.x, 30, 4, 262-265, 30(4), 262-265, 2003.04. |
| 124. |
N.Terahara, T.Matsui, K.Fukui, K.Matsugano, K.Sugita, and K.Matsumoto, Caffeoylsophorose in a red vinegar produced through fermentation with purple sweetpotato, J. Agric. Food Chem., 10.1021/jf026146u, 51, 9, 2539-2543, 51(9), 2539-2543, 2003.03. |
| 125. |
K.Matsumoto, K.Takayama, J.M.Kanthi Abesundara, and T.Matsui, Assay of a-glucosidase inhibitory activity using flow-biosensor system, Anal. Chim. Acta, 10.1016/S0003-2670(02)01539-8, 479, 2, 135-141, 479, 135-141, 2003.02. |
| 126. |
T.Matsui, S.Ebuchi, M.Kobayashi, K.Fukui, K.Sugita, N.Terahara, and K.Matsumoto, K.Matsumoto: Anti-hyperglycemic effect of diacylated anthocyanin derived from Ipomoea batatas cv. Ayamurasaki can be achieved through the a-glucosidase inhibitory action, J. Agric. Food Chem., 10.1021/jf025913m, 50, 25, 7244-7248, 50(25),7244-7248, 2002.12. |
| 127. |
Safety and depressor effect of sardine peptide-drink in mild hypertensives, high-normal hypertensives, and normotensives.. |
| 128. |
T.Matsui, K.Tamaya, E.Seki, K.Osajima, K.Matsumoto, and T.Kawasaki, Absorption of Val-Tyr with in vitro angiotensin I-converting enzyme inhibitory activity into the circulating blood system of mild hypertensive subjects., Biol. Pharmacol. Bull., 10.1248/bpb.25.1228, 25, 9, 1228-1230, 25(9), 1228-1230, 2002.09. |
| 129. |
T.Matsui, K.Tamaya, E.Seki, K.Osajima, K.Matsumoto, and T.Kawasaki, Circulatory and localized angiotensin I-converting enzyme inhibitory effects of the sardine peptide, Val-Tyr., J. Hypertension, 20, S241-S241, 22(suppl.4), S241, 2002.06. |
| 130. |
M.Imamura, T.Matsui, E.Seki, K.Osajima, K.Matsumoto, and T.Kawasaki, The relationship between rat age and the antihypertensive effect of the sardine peptide, Val-Tyr., J. Hypertension, 20, S241-S241, 22(suppl.4), S241, 2002.06. |
| 131. |
T.Kawasaki, Y.Fukushima, J.J.Chen, K.Kegai, E.Seki, K.Osajima, K.Itoh, T.Matsui, and K.Matsumoto, Antihypertensive effect of drink containing peptides derived from sardine protein hydrolysate in mild hypertensive blood pressure subjects., J. Hypertension, 20, S332-S332, 22(suppl.4), S332, 2002.06. |
| 132. |
Chun-Hui Li, T. Matsui, K. Matsumoto, R. Yamasaki, and T. Kawasaki, Latent production of angiotensin I-converting enzyme inhibitors from buckwheat protein., J. Peptide Sci., 10.1002/psc.387, 8, 6, 267-274, 8, 267-274, 2002.05. |
| 133. |
T.Matsui, A.Yukiyoshi, S.Doi, H.Sugimoto, H.Yamada, and K.Matsumoto, Gastrointestinal enzyme production of bioactive peptides from royal jelly protein and their antihypertensive ability in SHR., J. Nutr. Biochem., 10.1016/S0955-2863(01)00198-X, 13, 2, 80-86, 13(2), 80-86, 2002.04. |
| 134. |
Depressor effect of sardine peptide-drink in mild hypertensives and normotensives.. |
| 135. |
T.Matsui, K.Tamaya, E.Seki, K.Osajima, K.Matsumoto, and T.Kawasaki, Val-Tyr as a natural antihypertensive dipeptide can be absorbed into human circulatory blood system., Clin. Exp. Pharmacol. Physiol., 10.1046/j.1440-1681.2002.03628.x, 29, 3, 204-208, 29(3), 204-208, 2002.03. |
| 136. |
T.Matsui, M.Kobayashi, S.Hayashida, and K.Matsumoto, Luteolin, a flavone, does not suppress postprandial glucose absorption through an inhibition of a-glucosidase action., Biosci. Biotechnol. Biochem., 66, 3, 689-692, 66(3), 689-692, 2002.03. |
| 137. |
Mahmud Tareq Hassan Khan, T.Matsui, K.Matsumoto, and Shaila Jabbar, In vitro antihypertensive studies of some Bangladeshi plant extracts., Die Pharmazie, 56(11), 902-903, 2001.11. |
| 138. |
T.Matsui, T.Ueda, T.Oki, K.Sugita, N.Terahara, and K.Matsumoto, a-Glucosidase inhibitory action of natural acylated anthocyanins. 1. Survey of natural pigments with potent inhibitory activity., J. Agric. Food Chem., 10.1021/jf001251u, 49, 4, 1948-1951, 49(4), 1948-1951, 2001.04. |
| 139. |
T.Matsui, T.Ueda, T.Oki, K.Sugita, N.Terahara, and K.Matsumoto, a-Glucosidase inhibitory action of natural acylated anthocyanins. 2. a-Glucosidase inhibition by isolated acylated anthocyanins., J. Agric. Food Chem., 10.1021/jf0012502, 49, 4, 1952-1956, 49(4), 1952-1956, 2001.04. |
| 140. |
T.Oki, T.Matsui, and K. Matsumoto, Evaluation of a-glucosidase inhibition by using an immobilized assay system., Biol. Pharm. Bull., 23(9), 1084-1087, 2000.09. |
| 141. |
Y-H.Hwang, T.Matsui, T.Hanada, M.Shimoda, K.Matsumoto, and Y.Osajima, Desorption behavior of sorbed flavor compounds from packaging films with ethanol solution., J. Agric. Food Chem., 10.1021/jf991082h, 48, 9, 4310-4313, 48(9), 427-431, 2000.09. |
| 142. |
Deviation of desorption equation of flavors from plastic films by using compatibility theory.. |
| 143. |
T. Kawasaki, E. Seki, K. Osajima, M. Yoshida, K. Asada, T. Matsui, and Y. Osajima, Antihypertensive effect of Valyl-Tyrosine, a short chain peptide derived from sardine muscle hydrolyzate, on mild hypertensive subjects., J. Human Hypertens., 10.1038/sj.jhh.1001065, 14, 8, 519-523, 14, 519-523, 2000.08. |
| 144. |
T.Matsui, K.Tamaya, K.Matsumoto, E.Seki, K.Osajima, and T.Kawasaki, The bioavailability of antihypertensive small peptide, Val-Tyr, in human., J. Hypertension, 18, S87-S87, 18(suppl.4), S87, 2000.08. |
| 145. |
E.Seki, T.Kawasaki, K.Osajima, K.Tamaya, and T.Matsui, Antihypertensive effect of sardine peptide, Val-Tyr, in normotensive and hypertensive subjects., J. Hypertension, 18, S90-S90, 18(suppl.4), S90, 2000.08. |
| 146. |
Antihypertensive effect of sardine peptides in mild hypertensives and high-normal hypertensives.. |
| 147. |
T.Matsui, Chun-Hui Li, T. Tanaka, T. Maki, Y. Osajima, and K. Matsumoto, Depressor effect of wheat germ hydrolysate and its novel angiotensin I-converting enzyme inhibitory peptide, Ile-Val-Tyr, and the metabolism in rat and human plasma., Biol. Pharm. Bull., 23, 4, 427-431, 23(4), 427-431, 2000.04. |
| 148. |
Antihypertensive effect of sardine peptides and Val-Tyr - antihypertensive effect of the peptides in spontaneously hypertensive rats-. |
| 149. |
T.Matsui, K.Tamaya, K.Matsumoto, Y.Osajima, K.Uezono, and T.Kawasaki, Plasma concentrations of angiotensin metabolites in young male normotensive and mild hypertensive subjects., Hypertens. Res., 10.1291/hypres.22.273, 22, 4, 273-277, 22(4), 273-277, 1999.08. |
| 150. |
T.Matsui, K.Tamaya, T.Kawasaki, and Y.Osajima, Determination of angiotensin metabolites in human plasma by fluorimetric high-performance liquid chromatography using a heart-cut column-switching technique., J. Chromatogr.B, 10.1016/S0378-4347(99)00128-0, 729, 1-2, 89-95, 729, 89-95, 1999.07. |
| 151. |
T.Matsui, C. -H. Li, and Y.Osajima, Preparation and characterization of novel bioactive peptides responsible for angiotensin I-converting enzyme inhibition from wheat germ., J. Peptide Sci., 10.1002/(SICI)1099-1387(199907)5:7<289::AID-PSC196>3.0.CO;2-6, 5, 7, 289-297, 5, 289-297, 1999.05. |
| 152. |
P.Pfnuer, T.Matsui, W.Grosch, H.Guth, and P.Schieberle, Development of a stable isotope dilution assay for the quantification of 5-methyl-(E)-2-heptene-4-one: Application to hazelnut oils and hazelnuts., J. Agric. Food Chem., 10.1021/jf9810214, 47, 5, 2044-2047, 47(5), 2044-2047, 1999.05. |
| 153. |
T.Matsui, T. Oki, and Y.Osajima, Isolation and identification of peptidic a-glucosidase inhibitors derived from sardine mucle hydrolyzate., Z. Naturforsch., 54, 3-4, 259-263, 54C, 259-263, 1999.02. |
| 154. |
T.Oki, T.Matsui, and Y.Osajima, Inhibitory effect of a-glucosidase inhibitors varies according to its origin., J. Agric. Food Chem., 10.1021/jf980788t, 47, 2, 550-553, 47(2), 550-553, 1999.02. |
| 155. |
T.Oki, K.Matsumoto, T.Matsui, and Y.Osajima, Change in catalytic property of trypsin immobilized on support activated with glutaraldehyde in lower protogenic solvent., Food Sci. Technol. Res., 5(1), 13-17, 1999.01. |
| 156. |
T.Kawasaki, K.Osajima, E.Seki, M.Yoshida, K.Asada, T.Matsui, and Y.Osajima, Antihypertensive effect of Val-Tyr, a short chain peptide derived from sardine muscle hydrolyzate, on hypertensives., J. Hypertension, 16, S25-S25, 16(suppl.2), S25, 1998.06. |
| 157. |
T.Matsui, Y.Osajima, K.Uezono, and T.Kawasaki, Metabolism of endogenous Val-Tyr with antihypertensive effect in human plasma., J. Hypertension, 16, S138-S138, 16(suppl.2), S138, 1998.06. |
| 158. |
Antihypertensive effect of sardine peptides in mild hypertensive subjects.. |
