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Toshiro Matsui Last modified date:2024.02.15

Professor / Food Science and Biotechnology
Department of Bioscience and Biotechnology
Faculty of Agriculture

Graduate School
Undergraduate School
Administration Post
Director of the Research and Development Center for Taste and Odor Sensing

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Homepage for Research and Development Center for Five-Sense Devices .
Homepage for Lab. of Food Analysis, Division of Bioscience and Bioenvironmental Siceinces, Faculty of Agriculture .
Academic Degree
Country of degree conferring institution (Overseas)
Field of Specialization
Food Analysis, Functional Food Science
ORCID(Open Researcher and Contributor ID)
Total Priod of education and research career in the foreign country
Outline Activities
Studies on a prophylaxis of life-related diseases such as hypertension and diabetes by bioactive food compounds, and on their bioavailability.
Preparation of antihypertensive food compounds from natural resources, and elucidation of antihypertensive mechanism towards the renin-angiotensin systems.
Preparation of antihyperglycemic food compounds from natural resources, and elucidation of their actions such as suppression of glucose absorption and promotion of glucose assumption via inhibition of polyol metabolism.
Development of analytical assay systems of food compounds responsible for sensory properties.
(Alzheimer's dementia)
According to our first finding on the food factors showing prevention of AD dementia, the mechanism underlying the preventive effect and active food compounds are in progress.
Research Interests
  • Evaluation of Bioavailability of bioactive compounds
    keyword : Bioactive compounds, Intestinal absorption, Bioavailability
    2000.04Subject: prophylaxis of life-related disease such as hypertension and diabetes by specific active food compounds. The antihypertensive action by these compounds, in particular active peptides from sardine proteins, is also investigated by in vitro or in vivo systems..
  • Prevention of lefe-style related diseases by bioactive food compounds
    keyword : prevention of lyfe-style related disease, hypertension, diabetes, bioactive compounds
    2000.04Subject: prophylaxis of life-related disease such as hypertension and diabetes by specific active food compounds. The antihypertensive action by these compounds, in particular active peptides from sardine proteins, is also investigated by in vitro or in vivo systems..
  • Sorption of flavor compounds into polymer films
    keyword : flavor, sorption, food packaging
    1987.04Study on sorption or desorption behavior of flavor into food packaging..
Academic Activities
1. Mitsuru Tanaka and Toshiro Matsui, Biologically Active Peptides: from Basic Science to Applications for Human Health, Elsevier Inc., 2021.06.
2. Ju Qiu, Norihiko Terahara, 松井 利郎, Natural Bioactive Molecules: Impacts and Prospects, Narosa Publishing House, 2014.05.
3. Matsui Toshiro, Z. Wang, Mitsuru Tanaka, Bioactive Natural Products: Opportunities & Challenges in Medicinal Chemistry , World Scientific Publishing, pp.201-221, 2012.10.
4. T.Hasegawa, K.Matsumoto, T.Matsui, Laser Scanning, Theory and Applications, INTECH Book, pp.365-388, 2011.07.
5. T.Matsui, M.Tanaka, Biologically Active Food Proteins and Peptides in Health- Fundamental and Clinical aspects-, Wiley Blackwell, pp.43-54, 2010.07.
6. Norihiko Terahara and Toshiro Matsui, Recent Progress in Studies on Bioavailability and Health Promotion Effects, Research Signpost, Anthocyanins as food factor, 2008.01.
7. T.Matsui, K.Matsumoto, Current Topics in Peptide & Protein Research Vol.4, Research Trends, Bioactive Peptides with Antihypertensive Potency Derived from Natural Protein pp.113-122, 2001.04.
8. Food Function-antihypertensive effect of food compounds..
1. Tae Hun Hahm, Mitsuru Tanaka, Toshiro Matsui, Current knowledge on intestinal absorption of anthocyanins, J. Agric. Food Chem., 2022.01.
2. Toshiro Matsui, Polyphenols-Absorption and occurrence in the body system, Food Sci. Technol. Res., 28(1), 13-33 (2022), 2022.01.
3. Toshiro Matsui, Atsuko Yoshino, and Mitsuru Tanaka, A trip of peptides to the brain, Food Production, Processing and Nutrition,, 2020.12, [URL].
4. Huu Nghi Nguyen, Tae Hun Ham, Toshiro Matsui, Mitsuru Tanaka, Detection and visualization of food-derived polyphenols by matrix-assisted laser desorption/ionization mass spectrometry imaging, Sensors and Materials, 10.18494/SAM.2019.2371, 2019.01, Food-derived polyphenols have health benefits owing to their ability to prevent some diseases. Knowledge of their bioavailabilities and metabolism is important for understanding the mechanisms underlying their benefits. Thus, mass spectrometry (MS)-based analytical techniques, such as liquid chromatography-MS (LC-MS), that are capable of highly sensitive and simultaneous detection of absorbed polyphenols and their metabolites are essential tools in this field. Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) imaging is currently used in biological sciences for visualizing the spatial information of various compounds, such as peptides, proteins, lipids, drugs, and food compounds, in various biological tissues. However, the application of MALDI-MS imaging to polyphenols is challenging owing to the lack of appropriate matrix reagents for visualizing (or detecting) polyphenols absorbed in target biological tissues. This review highlights the development of the MALDI-MS imaging technique for the detection and visualization of food-derived polyphenols. First, the development of new matrices and matrix additives for the detection and visualization of polyphenols is discussed, followed by their introduction to inhibitor-aided MALDI-MS imaging for the elucidation of intestinal absorption routes and the metabolic behaviors of polyphenols. The MALDI-MS imaging technique shows great potential as a novel analytical tool for elucidating the bioavailabilities of food-derived polyphenols..
5. Weilin Shen, Toshiro Matsui, Intestinal absorption of small peptides
a review
, International Journal of Food Science and Technology, 10.1111/ijfs.14048, 2019.06, Peptides display diverse structural features because of their varied amino acid compositions. The structural diversity often imparts them complex physiological functions, or possible health-beneficial effects. Some small peptides (di-/tripeptides) exert preventive effects against conditions such as hypertension, hypercholesterolaemia and atherosclerosis. Despite their health benefits, a limited understanding of peptide absorption may hinder their extensive application. Therefore, this review briefly introduces the in vitro and in vivo findings on the intestinal absorption of small peptides and potential factors affecting their absorption..
6. Yoshinori Mine, Toshiro Matsui, Current understanding of bioaccessibility and bioavailability of food-derived bioactive peptides, International Journal of Food Science and Technology, 10.1111/ijfs.14164, 2019.07.
7. Toshiro Matsui, Are Peptides Absorbable Compounds?, Journal of Agricultural and Food Chemistry, 10.1021/acs.jafc.7b05589, 2018.01.
8. Weilin Shen, Toshiro Matsui, Current knowledge of intestinal absorption of bioactive peptides, Food & Function, 8, 4306-4314, 2017.10.
9. Toshiro Matsui, Condensed catechins and their potential health-benefits, Eur. J. Pharm., 75, 495-502, 2015.10.
10. Antihypertensive effect of bioactive peptides.
11. Peptidic food and hypertension.
1. Lihong Cheng, Mitsuru Tanaka, Atsuko Yoshino, Yuki Nagasato, Fuyuko Takata, Shinya Dohgu, Toshiro Matsui, A memory-improving dipeptide, Tyr-Pro, can reach the mouse brain after oral administration, Sci. Rep., 10.1038/s41598-023-44161-z, 13, 16908, 2023.10.
2. Nana Esaki, Toshiro Matsui, Takanori Tsuda, Lactate induces the development of beige adipocytes via an increase in the level of reactive oxygen species, Food Funct., 10.1039/d3fo03287f, 14, 9725-9733, 2023.08.
3. Keisuke Sanematsu, Masato Yamamoto, Yuki Nagasato, Yuko Kawabata, Yu Watanabe, Shusuke Iwata, Shingo Takai, Kiyoshi Toko, Toshiro Matsui, Naohisa Wada, Noriatsu Shigemura, Prediction of dynamic allostery for the transmembrane domain of the sweet taste receptor subunit, TAS1R3, Commun. Biol., 10.1038/s42003-023-04705-5, 6, 340, 2023.08.
4. Guanzhen Gao, Saya Nakamura, Sumire Asaba, Yuji Miyata, Hisayuki Nakayama, Toshiro Matsui, Hesperidin Preferentially Stimulates Transient Receptor Potential Vanilloid 1, Leading to NO Production and Mas Receptor Expression in Human Umbilical Vein Endothelial Cells, JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 10.1021/acs.jafc.2c04045, 70, 36, 2022.09, Here, the mechanism of vasorelaxant Mas receptor (MasR) expression elevated by hesperidin in spontaneously
hypertensive rats was investigated in human umbilical vein endothelial cells (HUVECs). HUVECs were cultured with 1 μM
hesperidin for 2 h, following the measurements of nitric oxide (NO) production and vasomotor-related receptors’ expression.
Hesperidin significantly promoted NO production (224.1 ± 18.3%, P was upregulated (141.2 ± 12.5%, P production. When a transient receptor potential vanilloid 1 (TRPV1) was knocked down by silencing RNA or Ca2+/calmodulindependent
kinase II (CaMKII) and p38 mitogen-activated protein kinase (p38 MAPK) were inhibited, the increased MasR
expression by hesperidin was abrogated. The inhibitions of CaMKII and endothelial NO synthase (eNOS) abolished the hesperidininduced
NO production. The structure−activity relationship analysis of flavonoids demonstrated that the B ring of the twisted
flavonoid skeleton with a hydroxy group at the 3′ position was a crucial factor for TRPV1 stimulation. Taken together, it was
demonstrated that hesperidin may stimulate TRPV1-mediated cascades, leading to the activation of two signaling axes, CaMKII/p38
MAPK/MasR expression and CaMKII/eNOS/NO production in HUVECs..
5. Chizumi Abe, Ayano Soma, Tint Ni Ni Tun, Ye Zhang, Yosuke Nishitani, Hiroyuki Kayaki, Hideaki Kawakami, Toshiro Matsui, Pharmacokinetic profiles of 3-(4-hydroxy-3-methoxyphenyl) propionic acid and its conjugates in Sprague-Dawley rats, Biosci. Biotechnol. Biochem, 87, 5, 516-524, 2023.05.
6. Yuna Lee, Akihiro Nakano, Yuki Nagasato, Takashi Ichinose, Toshiro Matsui, In Vitro and in Silico Analyses of the Adiponectin Receptor Agonistic Action of Soybean Tripeptides, JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 10.1021/acs.jafc.2c02115, 70, 25, 7695-7703, 2022.06, The Tyr-Pro (YP) dipeptide can serve as an adiponectin receptor 1 (AdipoR1) agonist. We thus investigated the
AdipoR1-agonistic potential of YP-related tripeptides in the soybean protein sequence. Among the 17 soybean candidate tripeptides,
those elongated at the C-terminus of YP (0.1 μM YPG, 140 ± 16%; 0.1 μM YPE, 141 ± 22%; 0.1 μM YPP, 145 ± 19%; 0.1 μM
YPQ, 143 ± 20%; p AdipoRon (163 ± 52%, p indicating that the two tripeptides had an AdipoR1 agonistic effect. CHARMM-GUI-aided molecular dynamics simulation in a
virtual phospholipid membrane revealed that YPG and YPP were stably positioned at the binding pockets of AdipoR1 (binding free
energy alternative adiponectin-like potential via their preferential binding to AdipoR1..
7. Mitsuru Tanaka, Keishiro Arima, Tomotaka Takeshita, Yuri Kunitake, Naoto Ohno, Miho Imamura, Toshiro Matsui, Laser desorption ionization-mass spectrometry with graphite carbon black nanoparticles for simultaneous detection of taste- and odor-active compounds, ACS Appl. Nano Mater.,, 5, 2187-2194, 2023.02, There is no analytical system to directly and simultaneously detect taste- and odor-active compounds. Graphite
carbon black (GCB) nanoparticles, a chemical adsorbent for a variety of compounds, including gaseous molecules, which consists of
nanoparticles made from multilayered sheets of sp2-conjugated atomic carbon, have the characteristics required for a surface-assisted
laser desorption ionization−mass spectrometry (SALDI-MS) material. Thus, in this study, GCB-LDI-MS was developed for the
simultaneous detection of taste- and odor-active compounds. The proposed GCB-LDI-MS successfully detected volatile compounds,
including ethyl esters, alcohols, fatty acids, and aldehydes with up to 16 carbons (maximum carbon number of the tested
compounds), which could not be detected by conventional matrix-assisted-LDI-MS. Moreover, a series of taste-active compounds
(e.g., amino acids and sugars) and various volatile compounds (e.g., ethyl esters, aldehydes, alcohols, fatty acids, and lactones) as a
mixture in liquid samples were also detected by the present GCB-LDI-MS. Thus, the proposed simple and rapid detection technique
can digitize the analysis of flavor compounds, which provides an analytical concept for flavor scanning to evaluate the palatability of
food products..
8. Misaki Ishida, Haruna Ide, Keishiro Arima, Zeyu Zhao, Toshiro Matsui, Kiyoshi Toko, Identification of the Principle of Taste Sensors to Detect Non-Charged Bitter Substances by H-1-NMR Measurement, SENSORS, 10.3390/s22072592, 22, 7, 2022.04.
9. Tae Hun Hahm, Toshiro Matsui, Mitsuru Tanaka, Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Imaging of Tissues via the Formation of Reproducible Matrix Crystals by the Fluorescence-Assisted Spraying Method: A Quantification Approach, ANALYTICAL CHEMISTRY, 10.1021/acs.analchem.1c03369, 94, 4, 1990-1998, 2022.02, The application of matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) imaging to
quantitative analyses is restricted by the variability of MS intensity of the analytes in nonreproducible matrix crystals of tissues. To
overcome this challenge, a fluorescence-assisted spraying method was developed for a constant matrix amount employing an MSdetectable
fluorescent reagent, rhodamine 6G (R6G), which was sprayed with the matrix. To form a homogeneous matrix crystal on
the tissue section, a matrix solution, 1,5-diaminonaphthalene (10 mg/mL), containing R6G (40 μg/mL) and O-dinitrobenzene (ODNB,
10 mg/mL) was sprayed until the desired constant fluorescence intensity was achieved. Compared with that obtained via
conventional cycle-number-fixed spraying [relative standard deviation (RSD) = 31.1%], the reproducibility of the relative MS
intensity of the analyte [ferulic acid (FA), RSD = 3.1%] to R6G was significantly improved by the fluorescence-assisted matrix
spraying. This result indicated that R6G could be employed as an index of the matrix amount and an MS normalizing standard. The
proposed matrix spraying successfully quantified nifedipine (0.5−40 pmol/mm2 in the positive mode, R2 = 0.965) and FA (0.5−75
pmol/mm2 in the negative mode, R2 = 0.9972) in the kidney section of a rat. Employing the quantitative MALDI-MS imaging assay,
FA, which accumulated in the kidney of the rat after 50 mg/kg was orally administered, was visually determined to be 3.5, 3.0, and
0.2 μmol/g tissue at 15, 30, and 60 min, respectively..
10. Ye Zhang, Chizumi Abe, Koji Ochiai, Toshiro Matsui, Tissue distribution of orally administered prenylated isoflavones, glyceollins, in Sprague-Dawley rats, J. Agric. Food Chem., 69, 49, 15165-15174, 2021.12, Apart from the physiological effects of glyceollins, information regarding their tissue distribution is scarce in the literature. Thus, the aim of this study is to clarify the distribution of glyceollins in rat organs. Glyceollins I and III were orally administered to Sprague-Dawley rats (1.0 mg/kg) with daidzein as control, and their accumulations in organs were investigated by liquid chromatography-time-of-flight/mass spectrometry (LC-TOF/MS). Glyceollins accumulated in intact and conjugated forms in circulatory organs with a Tmax of 0.5 h, in the following order of descending preference: liver, kidney, heart, lung, soleus muscle, and abdominal aorta. The accumulation of hydrophobic glyceollin I was more than 1.5 times higher than that of III. In contrast, daidzein and hydroxy equol were detected only in the liver and kidneys at lower concentrations (1/100 times) than those of glyceollins. In conclusion, prenylated isoflavones, glyceollins, were preferentially distributed in circulatory organs as intact, sulfated, or glucuronidated forms up to 6 h after the intake..
11. Xiaojing Sheng, Mitsuru Tanaka, Risa Katagihara, Marika Hashimoto, Satoshi Nagaoka, Toshiro Matsui, A novel approach for simultaneous analysis of peptide metabolites from orally administered glycinin in rat bloodstream by a coumarin-tagged MALDI-MS, J. Agric. Food Chem., 69, 49, 14840-14848, 2021.12.
12. Yuna Lee, Akihiro Nakano, Saya Nakamura, Kenta Sakai, Mitsuru Tanaka, Keisuke Sanematsu, Noriatsu Shigemura, Toshiro Matsui, In vitro and in silico characterization of adiponectin-receptor agonist dipeptides, npj Science of Food, 5, 29, 2021.11, The aim of this study is to develop a dipeptide showing an adiponectin receptor 1 (AdipoR1) agonistic effect in skeletal muscle L6 myotubes. Based on the structure of the AdipoR1 agonist, AdipoRon, 15 synthetic dipeptides were targeted to promote glucose uptake in L6 myotubes. Tyr-Pro showed a significant increase in glucose uptake among the dipeptides, while other dipeptides, including Pro-Tyr, failed to exert this effect. Tyr-Pro induces glucose transporter 4 (Glut4) expression in the plasma membrane, along with adenosine monophosphate-activated protein kinase (AMPK) activation. In AdipoR1-knocked down cells, the promotion by Tyr-Pro was ameliorated, indicating that Tyr-Pro may directly interact with AdipoR1 as an agonist, followed by the activation ofAMPK/Glut4 translocation in L6 myotubes. Molecular dynamics simulations revealed that a Tyr-Pro molecule was stably positioned in the two potential binding pockets (sites 1 and 2) of the seven-transmembrane receptor, AdipoR1, anchored in a virtual 1- palmitoyl-2-oleoyl-phosphatidylcholine membrane. In conclusion, we demonstrated the antidiabetic function of the Tyr-Pro dipeptide as a possible AdipoR1 agonist..
13. Kazunori Nakagawa, Mitsuru Tanaka, Tae Hun Hahm, Huu-Nghi Nguyen, Toshiro Matsui, Yong-Xiang Chen, Yutaka Nakashima, Accumulation of plasma-derived lipids in the lipid core and necrotic core of human atheroma: imaging mass spectrometry and histopathological analyses., Arterioscler. Thoromb. Vasc. Biol., 41, 11, e498-e511, 2021.11, OBJECTIVE: To clarify the pathogenesis of human atheroma, the origin of deposited lipids, the developmental mechanism of liponecrotic tissue, and the significance of the oxidation of phospholipids were investigated using mass spectrometry-aided
imaging and immunohistochemistry.
APPROACH AND RESULTS: Atherosclerotic lesions in human coronary arteries were divided into 3 groups: pathologic intimal thickening with lipid pool, atheroma with lipid core, and atheroma with necrotic core. The lipid pool and lipid core were characterized by the deposition of extracellular lipids. The necrotic core comprised extracellular lipids and liponecrotic tissue. The proportion of cholesteryl linoleate in cholesteryl linoleate+cholesteryl oleate fraction in the extracellular lipid and liponecrotic regions differed significantly from that of the macrophage foam cell–dominant region, and the plasma-derived components (apolipoprotein B and fibrinogen) were localized in the regions. The liponecrotic region was devoid of elastic and collagen fibers and accompanied by macrophage infiltration in the surrounding tissue. Non–oxidized phospholipid (Non-OxPL), OxPL, and Mox macrophages were detected in the three lesions. In the atheroma with lipid core and atheroma with necrotic core, non-OxPL tended to localize in the superficial layer, whereas OxPL was distributed evenly. Mox macrophages were colocalized with OxPL epitopes.
CONCLUSIONS: In human atherosclerosis, plasma-derived lipids accumulate to form the lipid pool of pathologic intimal thickening, lipid core of atheroma with lipid core, and necrotic core of atheroma with necrotic core. The liponecrotic tissue in
the necrotic core appears to be developed by the loss of elastic and collagen fibers. Non-OxPL in the accumulated lipids is oxidized to form OxPL, which may contribute to the lesion development through Mox macrophages.
14. Baorui Li, Lei Fu, Ruchia Kojima, Ayaka Yamamoto, Tomoya Ueno, Toshiro Matsui, Theaflavins prevent the onset of diabetes through ameliorating glucose tolerance mediated by promoted incretin secretion in spontaneous diabetic Torii rats, J. Funct. Foods, 86, 104702, 2021.11.
15. Chizumi Abe, Ye Zhang, Kazuhiro Takao, Kuni Sasaki, Koji Ochiai, Toshiro Matsui, Visualization analysis of glyceollin production in germinating soybeans by matrix-assisted laser desorption/ionization mass spectrometric imaging technique. J. Agric., J. Agric. Food Chem., 69, 25, 7057-7063, 2021.06.
16. Weilin Shen, Kiyomi Ono, Mitsuru Tanaka, Toshiro Matsui, Characteristics of electrospray-ionization detection of synthetic di- to penta-oligopeptides by amine derivatizations, Anal. Sci., 37, 11, 1629-1632, 2021.11.
17. Reiko Nakao, Weilin Shen, Yasuka Shimajiri, Kumiko Kainou, Yuki Sato, Anayt Ulla, Kohta Ohnishi, Miyuki Ninomiya, Ayako Ohno, Takayuki Uchida, Mitsuru Tanaka, Kazuhito Akama, Toshiro Matsui, Takeshi Nikawa, Oral intake of rice overexpressing ubiquitin ligase inhibitory pentapeptide prevents atrophy in denervated skeletal muscle, npj Science of Food, 5, 25, 2021.09.
18. Juneha Bak, Yoshiyuki Miyazaki, Hayato Nakano, Toshiro Matsui, Profiling sulfate content of polysaccharides in seaweed species using a ligand-assisted 1H-NMR assay, Food Sci. Technol. Res., 27, 3, 505-510, 2021.06.
19. Xiaojing Sheng, Satoshi Nagaoka, Marika Hashimoto, Yusuke Amiya, Masato Beppu, Kenichiro Tsukamoto, Emiko Yanase, Mitsuru Tanaka, and Toshiro Matsui, Identification of peptides in blood following oral administration of β-conglycinin to Wistar rats, Food Chemistry, 10.1016/j.foodchem.2020.128197, 2021.01, In this study, β-conglycinin (100 mg/kg) was orally administered to Wistar rats in order to identify peptides that may be derived from the protein in the blood. Plasma samples taken from the tail vein up to 8 h after admin- istration were analyzed by matrix-assisted laser desorption/ionization (MALDI) and liquid chromatography- time-of-flight (LC-TOF) mass spectrometry (MS). In total, 126 signals were detected by MALDI-MS. Among the signals, nine oligopeptides (SEL, KGPL, SILGA, DSEL, GDANI, SYFV, CLQSC, GEQPRPF, and LVINEGDA) were successfully identified as β-conglycinin-derived peptides by LC-TOF/MS at a plasma concentration of 0.75–756 pmol/mL. The results demonstrated that β-conglycinin could be the dietary source protein for the oligopeptides produced prior to entering the circulating bloodstream of rats..
20. Tae Hun Hahm, Mitsuru Tanaka, Huu-Nghi Nguyen, Ayaka Tsutsumi, Koichi Aizawa, and Toshiro Matsui, Matrix-assisted laser desorption/ionization mass spectrometry-guided visualization analysis of intestinal absorption of acylated anthocyanins in Sprague-Dawley rats, Food Chemistry, 10.1016/j.foodchem.2020.127586, 334, 127586, 2021.01, It is unknown whether intestinal absorption of acylated anthocyanins occurs in their intact or metabolized form. In this study, with the aid of matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) ima- ging, intestinal absorption of acylated anthocyanins was visually investigated. Anthocyanin extracts from purple carrots were orally administered to Sprague-Dawley rats. Acylated cyanidins were absorbed into portal and circulating blood systems in their intact form, and aglycon; cyanidin 3-O-(6-O-feruloyl-β-D-glucopyranosyl)- (1 → 6)-[β-D-xylopyranosyl-(1 → 2)]-β-D-galactopyranoside (Cy3XFGG), and showed a high absorption of 39.3 ± 0.1 pmol/mL-plasma at 60 min after administration. MALDI-MS imaging analysis of the rat jejunum membranes showed that an organic anion transporting polypeptide (OATP) transporter was involved in Cy3XFGG transport, while deacylated anthocyanins were incorporated through both the glucose transporter 2 and OATP routes. In conclusion, acylated anthocyanin, Cy3XFGG, can be absorbed in its intact form through intestinal OATP..
21. Baorui Li, Lei Fu, Chizumi Abe, Alexia M. Nectoux, Ayaka Yamamoto, and Toshiro Matsui, Theaflavins inhibit glucose transport across Caco-2 cells through the downregulation of the Ca2+/AMP-activated protein kinase-mediated glucose transporter SGLT1, J. Funct. Foods,, 75, 104273, 2020.12, The study investigated the effects of theaflavins, which are intestinally non-absorbable compounds, on intestinal
glucose transport in Caco-2 cells. 13C6-Glucose transport experiments clearly revealed that glucose transport
across Caco-2 cells was significantly (p μM theaflavins for 24 h, the expression of SGLT1 expression was significantly (p difference in GLUT2 expression was observed. The theaflavin-induced inhibition of glucose transport was
reversed by the inhibition of influx routes mediated by OATP and MCT transporters. A Wes analysis established
that theaflavin-induced phosphorylation of AMPK was significantly (p endoplasmic reticulum Ca -release and CaMKK β. These findings demonstrated for the first time that theaflavins can inhibit glucose transport across Caco-2 cell monolayers through the suppression of SGLT1 expression partly via the activation of the intracellular Ca2+/CaMKK β/AMPK signaling pathway..
22. Juneha Bak, Yoshiyuki Miyazaki, Hayato Nakano, and Toshiro Matsui, Ligand-aided 1H nuclear magnetic resonance spectroscopy for non-destructive estimation of sulfate content in sulfated saccharides, Analytical Sciences, 2020.12.
23. Guanzhen Gao, Chizumi Abe, Alexia M. Nectoux, Shu-Wei Huang, Yuji Miyata, Kazunari Tanaka, Takashi Tanaka, Haruo Yamamura, and Toshiro Matsui, Anti-hypertensive effect of hesperidin and hesperidin-containing fermented Mikan tea in spontaneously hypertensive rats, Food Science and Technology Research, 2020.11, The anti-hypertensive effects of hesperidin and hesperidin-containing fermented Mikan tea were investigated in 8-week-old spontaneously hypertensive rats (SHRs) at a dose of 50 mg/kg body weight for both, over 20 weeks. At the end of the protocol, a significant (P
24. Hiroyuki Kubota, Koji Sakamoto, and Toshiro Matsui, A confocal Raman microscopic visualization of small penetrants in cellulose acetate using a deuterium-labeling technique, Scientific Reports,, 10, 16426, 2020.10.
25. Akira Tsujita, Asami Nagasaka, Hidehiko Okazaki, Shin Ogawa, Akinaga Gohda, and Toshiro Matsui, Quantitative determination of H2 in human blood by 22Ne-aided gas chromatography-mass spectrometry using a single quadrupole instrument, Analytical Sciences, 2020.10.
26. Mitsuru Tanaka, Chung Hsuan, Masataka Oeki, Weilin Shen, Asuka Goda, Yusuke Tahara, Takeshi Onodera, Keisuke Sanematsu, Tomotsugu Rikitake, Eiji Oki, Yuzo Ninomiya, Rintaro Kurebayashi, Hideto Sonoda, Yoshihiko Maehara, Kiyoshi Toko, Toshiro Matsui, Identification of characteristic compounds of moderate volatility in breast cancer cell lines, PloS one, 10.1371/journal.pone.0235442, 15, 6, e0235442, 2020.06, In this study, we were challenging to identify characteristic compounds in breast cancer cell lines. GC analysis of extracts from the culture media of breast cancer cell lines (MCF-7, SK-BR-3, and YMB-1) using a solid-phase Porapak Q extraction revealed that two compounds of moderate volatility, 1-hexadecanol and 5-(Z)-dodecenoic acid, were detected with markedly higher amount than those in the medium of fibroblast cell line (KMST-6). Furthermore, LC-TOF/MS analysis of the extracts clarified that in addition to the above two fatty acids, the amounts of five unsaturated fatty acids [decenoic acid (C10:1), decadienoic acid (C10:2), 5-(Z)-dodecenoic acid (C12:1), 5-(Z)-tetradecenoic acid (C14:1), and tetradecadienoic acid (C14:2)] in MCF-7 medium were higher than those in medium of KMST-6. Interestingly, H2O2-oxidation of 5-(Z)-dodecenoic acid and 5-(Z)-tetradecenoic acid produced volatile aldehydes that were reported as specific volatiles in breath from various cancer patients, such as heptanal, octanal, nonanal, decanal, 2-(E)-nonenal, and 2-(E)-octenal. Thus, we concluded that these identified compounds over-produced in breast cancer cells in this study could serve as potential precursors producing reported cancer-specific volatiles..
27. Ye Zhang, Kazuhiro Takao, Chizuru Abe, Kuni Sasaki, Koji Ochiai, and Toshiro Matsui, Intestinal absorption of prenylated isoflavones, glyceollins, in Sprague-Dawley rats., Journal of Agricultural and Food Chemistry, 10.1021/acs.jafc.0c02475, 68, 31, 8205-8211, 2020.06.
28. Takashi Ichinose, Hiroyasu Murasawa, Tomoko Ishijima, Shinji Okada, Keiko Abe, Saki Matsumoto, Toshiro Matsui, Shigeki Furuya, Tyr-Trp administration facilitates brain norepinephrine metabolism and ameliorates a short-term memory deficit in a mouse model of Alzheimer’s disease, PloS one, 10.1371/journal.pone.0232233, 15, 5, e0232233, 2020.05, The physiological actions of orally ingested peptides on the brain remain poorly understood. This study examined the effects of 39 orally administered synthetic Tyr-containing dipeptides on the enhancement of brain norepinephrine metabolism in mice by comparing the concentration of 3-methoxy-4-hydroxyphenylethyleneglycol (MHPG). Although Tyr-Tyr administration increased blood and cerebral cortex (Cx) Tyr concentrations the most, Tyr-Trp increased Cx MHPG concentration the most. The oral administration of Tyr-Trp ameliorated a short-term memory deficit of a mouse model of cognitive dysfunction induced by amyloid beta peptide 25–35. Gene expression profiling of mouse brain using a microarray indicated that Tyr-Trp administration led to a wide variety of changes in mRNA levels, including the upregulation of genes encoding molecules involved in catecholamine metabolism. A comparative metabolome analysis of the Cx of mice given Tyr-Trp or Tyr-Tyr demonstrated that Tyr-Trp administration yielded higher concentrations of Trp and kynurenine pathway metabolites than Tyr-Tyr administration, as well as higher L-dopa levels, which is the initial product of catecholamine metabolism. Catecholamines were not significantly increased in the Cx of the Tyr-Tyr group compared with the Tyr-Trp group, despite a marked increase in Tyr. Presumably, Tyr-Trp administration enhances catecholamine synthesis and metabolism via the upregulation of genes involved in Tyr and Trp metabolism as well as metabolites of Tyr and Trp. These findings strongly suggest that orally ingested Tyr-Trp modulates the brain metabolome involved in catecholamine metabolism and contributes to higher brain function..
29. Mitsuru Tanaka, Hayato Kiyohara, Atsuko Yoshino, Akihiro Nakano, Fuyuko Takata, Shinya Dohgu, Yasufumi Kataoka, Toshiro Matsui, Brain-transportable soy dipeptide, Tyr-Pro, attenuates amyloid β peptide25-35-induced memory impairment in mice, npj Science of Food, 10.1038/s41538-020-0067-3, 4, 1, 7-10, 2020.04, In this study, experiments on amyloid β peptide25-35-induced mice were performed to provide in vivo evidence on the potential of the blood–brain barrier transportable soy dipeptide, Tyr-Pro, in combating memory impairment. We demonstrated for the first time that oral administration of Tyr-Pro (100 mg/kg, twice a day) in mice for 16 days significantly improved impaired memory by spontaneous alternation and shortened step-through latency in amyloid β-induced mice..
30. Kazunari Tanaka, Sakiko Yamamoto, Katsuhisa Omagari, Masahiro Yuasa, Aoi Tsuchihashi, Yuji Miyata, Yutaka Yoshino, Hiroyuki Ono, Toshiro Matsui, Haruo Yamamura, Effects of Feeding Hesperidin Derived from Fermented Tea Leaves Made by Tea-rolling Processing of Thinned Satsuma Mandarin Fruit and Green Tea Leaves on Blood Pressure-Randomized, Double-blind, Placebo-controlled, Parallel-group Comparative Study, Japanese Pharmacology and Therapeutics, 48, 2, 225-235, 2020.02, Objectives We developed a fermented tea made by mixing thinned satsuma Mandarin fruit and green tea leaves at the level of 1 =3. This fermented tea contained hesperidin which has the high water-soluble property. We investigated the effects of feeding hesperidin derived from fer-mented tea leaves for 12 weeks on blood pressure in volunteers. Methods Thirty nine Subjects with high-normal blood pressure and stage I hypertension participated in a randomized, double-blind, placebo-controlled, parallel-group comparative test. Subjects consumed test beverage containing 1.20g fermented tea leaves or placebo beverAâge without tea leaves for 12 weeks. Systolic and diastolic blood pressures were measured every 4 weeks. Results Beverage containing fermented tea leaves reduced systolic blood pressures after the start of feeding. Also, systolic blood pressures were significantly lower in the test beverage group than in the placebo beverage group during feeding period. Conclusions The results suggested that feeding hesperidin derived from fermented lea leaves made by mixing thinned satsuma Mandarin fruit and tea leaves has an effect decreasing systolic blood pressures in subjects with high-nomial blood pressure and stage I hypertension..
31. Hsuan Chung, Satoshi Tajiri, Mai Hyoguchi, Riho Koyanagi, Akihiro Shimura, Fuyuko Takata, Shinya Dohgu, Toshiro Matsui, Analysis of catecholamine and their metabolites in mice brain by liquid chromatography-mass spectrometry using sulfonated mixed-mode copolymer column, analytical sciences, 10.2116/analsci.18P494, 35, 4, 433-439, 2019.01, In this study, a simultaneous assay for catecholamines and their metabolites in the brain was established using liquid chromatography-mass spectrometry (LC-MS). To achieve complete separation, a cation-exchange/reversed-phase mixedmode copolymer resin column containing 0.81 wt% sulfo groups was used for the simultaneous LC-MS assay. The analyzed catecholamines were dopamine (DA), norepinephrine (NE), and epinephrine (E), while the metabolites lacking amino groups were 3,4-dihydroxyphenylacetic acid (DOPAC), homovanillic acid (HVA), and 3-methoxy-4- hydroxyphenylglycol (MHPG). The metabolites were separated and detected using LC-MS, on columns with and without sulfo groups. However, we could not achieve adequate separation of catecholamines on both columns using a gradient elution of 0 - 50 (v/v)% methanol containing 0.1 (v/v)% formic acid (FA). When volatile ion-pairing reagents were added to the mobile phase, they improved the retention and detection of catecholamines on the sulfonated mixed-mode column. Under optimized elution conditions, which involved a linear gradient elution of water containing 0.1 (v/v)% FA to 50 (v/v)% acetonitrile in 50 mM ammonium formate at 40°C and a 0.20 mL/min rate, all six target molecules were simultaneously detected within 25 min, when using negative mode LC-MS on a sulfonated mixed-mode column. The limits of detection (LODs) for DA, NE, E, DOPCA, HVA, and MHPG were determined to be 20.7, 12.6, 74.6, 1110, 18.7, and 3196 nM, respectively. Moreover, the established LC-MS assay allowed the detection of endogenous DA, NE, and HVA, in normal mouse brain samples at concentrations higher than 20, 9, and 4 pmol/mg, respectively..
32. Alexia M. Nectoux, Chizumi Abe, Shu Wei Huang, Naoto Ohno, Junji Tabata, Yuji Miyata, Kazunari Tanaka, Takashi Tanaka, Haruo Yamamura, Toshiro Matsui, Absorption and Metabolic Behavior of Hesperidin (Rutinosylated Hesperetin) after Single Oral Administration to Sprague-Dawley Rats, Journal of Agricultural and Food Chemistry, 10.1021/acs.jafc.9b03594, 67, 35, 9812-9819, 2019.09, We investigated the absorption and metabolic behavior of hesperidin (hesperetin-7-O-rutinoside) in the blood system of Sprague-Dawley rats by liquid chromatography- and matrix-assisted laser desorption ionization mass spectrometries (LC-MS and MALDI-MS). After a single oral administration of hesperidin (10 mg/kg), which was expected to be absorbed in its degraded hesperetin form, we detected intact hesperidin in the portal vein blood (tmax, 2 h) for the first time. We successfully detected glucuronized hesperidin in the circulating bloodstream, while intact hesperidin had disappeared. Further MS analyses revealed that homoeriodictyol and eriodictyol conjugates were detected in both portal and circulating blood systems. This indicated that hesperidin and/or hesperetin are susceptible to methylation and demethylation during the intestinal membrane transport process. Sulfated and glucuronized metabolites were also detected in both blood systems. In conclusion, hesperidin can enter into the circulating bloodstream in its conjugated forms, together with the conjugated forms of hesperetin, homoeriodictyol, and/or eriodictyol..
33. Huu Nghi Nguyen, Mitsuru Tanaka, Baorui Li, Tomoya Ueno, Hideki Matsuda, Toshiro Matsui, Novel in situ visualisation of rat intestinal absorption of polyphenols via matrix-assisted laser desorption/ionisation mass spectrometry imaging, Scientific reports, 10.1038/s41598-019-39405-w, 9, 1, 2019.12, Matrix-assisted laser desorption/ionisation mass spectrometry imaging (MALDI-MSI) is presently used in physiological evaluations for visualisation of targets in organs. In the present study, MALDI-MSI was used as a visualisation technique to investigate the intestinal absorption of polyphenols. Nifedipine/phytic acid-aided MALDI-MSI was performed to visualise theaflavin-3′-O-gallate (TF3′G) and epicatechin-3-O-gallate (ECG) in the rat jejunum for 50-µM, 60-min transport experiments. Non-absorbable TF3′G was successfully visualised at the apical region, whereas absorbable ECG was detected throughout the rat jejunum. MALDI-MSI was also performed to determine the transport routes of the target metabolites. Signals corresponding to TF3′G and ECG in the membranes were diminished following treatment with inhibitors targeting the monocarboxylic acid transporter and organic anion transporting polypeptides. Enhanced visualisation of TF3′G was achieved by inhibiting efflux routes. Our findings demonstrated that the present MALDI-MSI can provide critical spatial informations on intestinal absorption of targets, by which TF3′G and ECG were incorporated into intestinal tissues, followed by efflux back to the apical compartment. In addition, MALDI-MSI analyses suggested that TF3′G was resistant to phase II metabolism during the influx/efflux processes, whereas ECG was susceptible to methylation and sulphation reactions. In conclusion, inhibitor-aided MALDI-MSI could serve as a powerful in situ visualisation technique for verifying intestinal transport routes and investigating the metabolism of penetrants..
34. Mitsuru Tanaka, Shinya Dohgu, Genki Komabayashi, Hayato Kiyohara, Fuyuko Takata, Yasufumi Kataoka, Takashi Nirasawa, Motohiro Maebuchi, Toshiro Matsui, Brain-transportable dipeptides across the blood-brain barrier in mice, Scientific reports, 10.1038/s41598-019-42099-9, 9, 1, 2019.12, Apart from nutrients required for the brain, there has been no report that naturally occurring peptides can cross the blood-brain barrier (BBB). The aim of this study was to identify the BBB-transportable peptides using in situ mouse perfusion experiments. Based on the structural features of Gly-N-methylated Gly (Gly-Sar), a reported BBB-transportable compound, 18 dipeptides were synthesized, and were perfused in the mouse brain for two minutes. Among the synthesized dipeptides, Gly-Sar, Gly-Pro, and Tyr-Pro were transported across the BBB with K
values of 7.60 ± 1.29, 3.49 ± 0.66, and 3.53 ± 0.74 µL/g·min, respectively, and accumulated in the mouse brain parenchyma. Additionally, using MALDI-MS/MS imaging analysis of Tyr-Pro-perfused brain, we provide evidence for Tyr-Pro accumulation in the hippocampus, hypothalamus, striatum, cerebral cortex, and cerebellum of mouse brain..
35. Anti-diabetic Effect of Acetic Acid-Free Red Vinegar in Spontaneously Diabetic Torii Rats.
36. Tatsuro Yamamoto, Chiyomi Sakamoto, Hiroaki Tachiwana, Mitsuru Kumabe, Toshiro Matsui, Tadatoshi Yamashita, Masatoshi Shinagawa, Koji Ochiai, Noriko Saitoh, Mitsuyoshi Nakao, Endocrine therapy-resistant breast cancer model cells are inhibited by soybean glyceollin I through Eleanor non-coding RNA, Scientific reports, 10.1038/s41598-018-33227-y, 8, 15202, 2018.10, Long-term estrogen deprivation (LTED) of an estrogen receptor (ER) α-positive breast cancer cell line recapitulates cancer cells that have acquired estrogen-independent cell proliferation and endocrine therapy resistance. Previously, we have shown that a cluster of non-coding RNAs, Eleanors (ESR1 locus enhancing and activating non-coding RNAs) formed RNA cloud and upregulated the ESR1 gene in the nuclei of LTED cells. Eleanors were inhibited by resveratrol through ER. Here we prepared another polyphenol, glyceollin I from stressed soybeans, and identified it as a major inhibitor of the Eleanor RNA cloud and ESR1 mRNA transcription. The inhibition was independent of ER, unlike one by resveratrol. This was consistent with a distinct tertiary structure of glyceollin I for ER binding. Glyceollin I preferentially inhibited the growth of LTED cells and induced apoptosis. Our results suggest that glyceollin I has a novel role in LTED cell inhibition through Eleanors. In other words, LTED cells or endocrine therapy-resistant breast cancer cells may be ready for apoptosis, which can be triggered with polyphenols both in ER-dependent and ER-independent manners..
37. Ayano Shimizu, Takakazu Mitani, Sachi Tanaka, Hiroshi Fujii, Motohiro Maebuchi, Yusuke Amiya, Mitsuru Tanaka, Toshiro Matsui, Soichiro Nakamura, Shigeru Katayama, Soybean-Derived Glycine-Arginine Dipeptide Administration Promotes Neurotrophic Factor Expression in the Mouse Brain, Journal of Agricultural and Food Chemistry, 10.1021/acs.jafc.8b01581, 66, 30, 7935-7941, 2018.08, Brain-derived neurotrophic factor (BDNF), a member of the neurotrophin family, plays an important role in cognitive abilities, including memory and learning. We demonstrated that soybean protein hydrolysate (SPH) diet suppresses age-related cognitive decline via the upregulation of BDNF in a mouse model of senescence. Our purpose was to identify novel bioactive peptides in SPH, which enhance BDNF expression. We treated mouse primary astrocytes with SPH as well as with its positively charged chromatographic fraction. Significant increases in the expression of BDNF were observed in the treatment with positively charged fraction of SPH. Among the synthesized peptides, the dipeptide glycine-arginine (GR) increased BDNF expression in vitro, and LC-TOF-MS analysis showed the presence of GR in the SPH. Furthermore, its administration in vivo increased the expression of BDNF in the cerebral cortex and the number of neurons in hippocampus and cerebral cortex. These data indicate that GR might promote neurogenesis by upregulating BDNF levels..
38. Hsuan Chung, Akihiro Shimura, Toshiro Matsui, Discriminant and simultaneous HPLC analysis of reducing and nonreducing monosaccharides on a polyethyleneimine-attached hydrophilic interaction liquid chromatography column, Food Science and Technology Research, 10.3136/fstr.24.501, 24, 3, 501-508, 2018.06, The aim of this study was to develop an analytical method for the discrimination of reducing and non-reducing monosaccharides including rare sugars on a prototype hydrophilic interaction liquid chromatography (HILIC) column. The HILIC column was composed of a glycidyl methacrylate-ethylene glycol dimethacrylate copolymer attached to 8 wt% polyethyleneimine. Seven monosaccharides (reducing aldoses: D-xylose, D-allose, and D-glucose; non-reducing ketoses: D-fructose, D-psicose, D-sorbose, and D-tagatose) were targeted. In individual HPLC analysis of both aldoses and ketoses, elution with 90 v/v% acetonitrile permitted successful detection and separation only for the non-reducing ketoses. Besides, elution of a mixture of ketoses and aldoses with 85 v/v% acetonitrile containing 5 mmol/L sodium 1-octanesulfonate (OS) (pH 4.8) enabled the simultaneous separation of all seven monosaccharides within 40 min. The polyethyleneimine-attached HILIC column allowed discriminant HPLC analysis for non-reducing ketoses using elution with 90 v/v% acetonitrile, whereas elution with 85 v/v% acetonitrile containing 5 mmol/L OS simultaneously detected both ketoses and aldoses..
39. Ryoko Yamamoto, Hisanori Minami, Hiromi Matsusaki, Mami Sakashita, Naoki Morita, Osamu Nishimiya, Naonobu Tsutsumi, Masashi Hosokawa, Yutaka Itabashi, Toshiro Matsui, Kazuhiro Ura, Consumption of the edible sea urchin Mesocentrotus nudus attenuates body weight gain and hepatic lipid accumulation in mice, Journal of Functional Foods, 10.1016/j.jff.2018.04.063, 47, 40-47, 2018.04, The beneficial health effects of edible sea urchin consumption in mice fed a normal (ND) or high-fat diet (HFD) were investigated in this study. Notably, sea urchin-administered (250, 500, 1000 mg/kg) mice exhibited lower body, liver, and visceral fat weights, lower plasma levels of aspartate aminotransferase and alanine aminotransferase, and lower hepatic triacylglycerol levels than those fed carboxymethyl cellulose (CMC). Despite the high levels of cholesterol found in sea urchins, intake of these organisms had no effect on plasma cholesterol levels among the mice tested. Additionally, sea urchin consumption resulted in enhanced levels of arachidonic acid and docosahexaenoic acid within mouse livers. Lastly, mice fed HFD with sea urchin (500 mg/kg) exhibited increased mRNA expression of uncoupling protein-1 within brown adipose tissue, compared with those fed HFD with CMC. In conclusion, consumption of sea urchin might provide a protective effect against the development of obesity and/or nonalcoholic fatty liver disease..
40. Baorui Li, Yusuke Terazono, Naoto Hirasaki, Yuki Tatemichi, Emiko Kinoshita, Akio Obata, Toshiro Matsui, Inhibition of Glucose Transport by Tomatoside A, a Tomato Seed Steroidal Saponin, through the Suppression of GLUT2 Expression in Caco-2 Cells, Journal of Agricultural and Food Chemistry, 10.1021/acs.jafc.7b06078, 66, 6, 1428-1434, 2018.02, We investigated whether tomatoside A (5α-furostane-3β,22,26-triol-3-[O-β-d-glucopyranosyl (1→2)-β-d-glucopyranosyl (1→4)-β-d-galactopyranoside] 26-O-β-d-glucopyranoside), a tomato seed saponin, may play a role in the regulation of intestinal glucose transport in human intestinal Caco-2 cells. Tomatoside A could not penetrate through Caco-2 cell monolayers, as observed in the transport experiments using liquid chromatography-mass spectrometry. The treatment of cells with 10 μM tomatoside A for 3 h resulted in a 46.0% reduction in glucose transport as compared to untreated cells. Western blotting analyses revealed that tomatoside A significantly (p
41. Si Jing Chen, Chiwa Aikawa, Risa Yoshida, Tomoaki Kawaguchi, Toshiro Matsui, Anti-prediabetic effect of rose hip (Rosa canina) extract in spontaneously diabetic Torii rats, Journal of the Science of Food and Agriculture, 10.1002/jsfa.8254, 97, 12, 3923-3928, 2017.09, BACKGROUND: Prediabetes, a high-risk state for developing diabetes showing impaired glucose tolerance but a normal fasting blood glucose level, has an increasing prevalence worldwide. However, no study investigating the prevention of impaired glucose tolerance at the prediabetic stage by anti-diabetic functional foods has been reported. Thus, the present study aimed to evaluate the anti-prediabetic effect of rose hip in a prediabetic rat model. RESULTS: Spontaneously diabetic Torii (SDT) rats were supplemented with hot-water extract of rose hip at a dose of 100 mg kg–1 body weight day–1 for 12 weeks. The results obtained showed that the supplementation of rose hip extract improved impaired glucose tolerance, promoted insulin secretion, preserved pancreatic beta-cell function and suppressed plasma advanced glycation end-products formation of methylglyoxal-derived hydroimidazolone (MG-H1) residue and Nϵ-carboxymethyl-lysine residues (e.g. MG-H1, control: 465.5 ± 43.8 versus rose hip: 59.1 ± 13.0 pmol mg protein–1, P
42. Vu Thi Hanh, Weilin Shen, Mitsuru Tanaka, Aino Siltari, Riita Korpela, Toshiro Matsui, Effect of Aging on the Absorption of Small Peptides in Spontaneously Hypertensive Rats, Journal of Agricultural and Food Chemistry, 10.1021/acs.jafc.7b01727, 65, 29, 5935-5943, 2017.07, In the present study, we aimed to evaluate the effect of aging on the absorption of small peptides in spontaneously hypertensive rats (SHRs). Three kinds of dipeptides, glycyl-sarcosine (Gly-Sar), Trp-His, and captopril (a dipeptidomimetic drug), a Gly-Sar-Sar tripeptide, a Gly-Sar-Sar-Sar tetrapeptide, and a Gly-Sar-Sar-Sar-Sar pentapeptide were administered at doses of 10 mg/kg each to 8- and 40-week-old SHRs. The peptides were all detected in their intact forms in the blood. There was a significantly promoted absorption of di/tripeptides in aged SHRs compared with young SHRs. In contrast, the absorption of tetra/pentapeptides was not affected by aging. PepT1 expression in the mid-jejunum was significantly increased in 40-week-old SHRs compared with 8-week-old SHRs, whereas aging did not alter the expression of claudin-1, a tight junction related protein. Thus, the present results suggest that SHR aging may enhance the absorption of di/tripeptides through the enhanced PepT1 transport route, although oligopeptides may be absorbed in an age-independent manner..
43. Gonzalo Miyagusuku-Cruzado, Naoki Morishita, Keiichi Fukui, Norihiko Terahara, Toshiro Matsui, Anti-prediabetic effect of 6-O-caffeoylsophorose in prediabetic rats and its stimulation of glucose uptake in L6 Myotubes, Food Sci. Technol. Res., 23, 3, 449-456, 2017.06.
44. Toshihiko Fukuda, Takanori Kuroda, Miki Kono, Mai Hyoguchi, Satoshi Tajiri, Mitsuru Tanaka, Yoshinori Mine, Matsui Toshiro, Adenine attenuates the Ca2+ contraction-signaling pathway via adenine receptor-mediated signaling in rat vascular smooth muscle cells, NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY, 10.1007/s00210-016-1264-0, 389, 9, 999-1007, 2016.09.
45. Thanutchaporn Kumrungsee, Sayaka Akiyama, Jian Guo, Mitsuru Tanaka, Matsui Toshiro, Identification of peptides in wheat germ hydrolysate that demonstrate calmodulin-dependent protein kinase II inhibitory activity, FOOD CHEMISTRY, 10.1016/j.foodchem.2016.06.059, 213, 329-335, 2016.12.
46. THuu-Nghi Nguyen, Mitsuru Tanaka, Genki Komabayashi, Matsui Toshiro, The photobase generator nifedipine as a novel matrix for the detection of polyphenols in matrix-assisted laser desorption/ionization mass spectrometry, JOURNAL OF MASS SPECTROMETRY, 10.1002/jms.3805, 51, 10, 748-756, 2016.10.
47. Akiko Mizokami, DaGuang Wang, Genki Komabayashi, Mitsuru Tanaka, Jing Gao, Hiroshi Takeuchi, Matsui Toshiro, Hirata Masato, An extract from pork bones containing osteocalcin improves glucose metabolism in mice by oral administration., Bioscience, Biotechnology, and Biochemistry, 10.1080/09168451.2016.1214530, 80, 11, 2176-2183, 2016.07.
48. Toshihiko Fukuda, Kaustav Majumder, Hua Zhang, Patricia V Turner, Matsui Toshiro, Yoshinori Mine, Adenine Inhibits TNF-alpha Signaling in Intestinal Epithelial Cells and Reduces Mucosal Inflammation in a Dextran Sodium Sulfate-Induced Colitis Mouse Model, JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 10.1021/acs.jafc.6b00665, 64, 21, 4227-4234, 2016.06.
49. Jian Guo, Naoto Hirasaki, Yuji Miyata, Kazunari Tanaka, TakashiTanaka, Xiao Wu, Yusuke Tahara, Kiyoshi Toko, Matsui Toshiro, Evaluating the Reduced Hydrophobic Taste Sensor Response of Dipeptides by Theasinensin A by Using NMR and Quantum Mechanical Analyses, PLOS ONE, 10.1371/journal.pone.0157315, 11, 6, e0157315, 2016.06, 重合カテキンであるテアシネンシンAが苦みペプチドである各種のトリプトファン含有ジペプチドの苦み応答を抑制し、かつ園現象は溶液内での両者の複合体形成であることをin vitroおよびin silico解析によって突き止めたこと。.
50. Hirosuke Inoue, Hisanori Nishio, Hidetoshi Takada, Etsuko Nanishi, Mitsuho Onimaru, Si Jing Chen, Matsui Toshiro, Toshiro Hara, Activation of Nod1 signaling induces fetal growth restriction and death through fetal and maternal vasculopathy, Journal of Immunology, 196, 2779-2787, 2016.05.
51. Thanutchaporn Kumrungsee, Sayaka Akiyama, Tomomi Saiki, Masato Omae, Kazuhiro Hamasawa, Toshiro Matsui, Vasorelaxant effect of 5′-methylthioadenosine obtained from candida utilis yeast extract through the suppression of intracellular Ca2+ concentration in isolated rat aorta, Journal of Agricultural and Food Chemistry, 10.1021/acs.jafc.6b00679, 64, 17, 3362-3370, 2016.05, Our study is the first to demonstrate the vasorelaxant effect of Candida utilis yeast extract on rat aorta (EC50 of 7.2 ± 3.2 mg/mL). Among five identified compounds, 5′-methylthioadenosine (MTA) exhibited comparable vasorelaxant effect (EC50 of 190 ± 40 μM) with adenosine, a known vasodilator, on 1 μM phenylephrine (PE)-contracted Sprague-Dawley rat aortic rings. MTA induced vasorelaxation in an endothelium-independent manner and independent of the adenosine receptors. MTA reduced a CaCl2-induced vasocontraction stimulated by 1 μM PE, whereas the effect was abolished in a 60 mM KCl-induced vasocontraction. This indicates that MTA was not involved in the suppression of extracellular Ca2+ influx. MTA significantly (P 2+ channel (VDCC). In conclusion, the underlying mechanism(s) of MTA-induced vasorelaxation involves the inhibition of Ca2+/CaMK II/VDCC phosphorylation pathway, resulting in the suppression of intracellular Ca2+ concentration in aortic rings..
52. Seong-Min Hong, Mitsuru Tanaka, Riho Koyanagi, Weilin Shen, Matsui Toshiro, Structural Design of Oligopeptides for Intestinal Transport Model, JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 10.1021/acs.jafc.6b00279, 64, 10, 2072-2079, 2016.03.
53. Vu Thi Hanh, Yutaro Kobayashi, Motohiro Maebuchi, Toshihiro Nakamori, Mitsuru Tanaka, Matsui Toshiro, Quantitative mass spectrometric analysis of dipeptides in protein hydrolysate by a TNBS derivatization-aided standard addition method, FOOD CHEMISTRY, 10.1016/j.foodchem.2015.05.053, 190, 345-350, 2016.01.
54. Yusuke Ushida, Boonyapichest Chutinan, Hiroyuki Suganuma, Mitsuru Tanaka, Matsui Toshiro, Paracellular Transport of Sulforaphane across Caco-2 Cell Monolayers, FOOD SCIENCE AND TECHNOLOGY RESEARCH, 10.3136/fstr.22.127, 22, 1, 127-134, 2016.01.
55. Jian Zhao, Aki Suyama, Hsuan Chung, Toshihiko Fukuda, Mitsuru Tanaka, Matsui Toshiro, Ferulic acid enhances nitric oxide production through up-regulation of argininosuccinate synthase in inflammatory human endothelial cells, LIFE SCIENCES, 10.1016/j.lfs.2015.12.044, 145, 224-232, 2016.01.
56. Toshihiko Fukuda, Takanori Kuroda, Miki Kono, Mai Hyoguchi, Mitsuru Tanaka, Matsui Toshiro, Augmentation of ferulic acid-induced vasorelaxation with aging and its structure importance in thoracic aorta of spontaneously hypertensive rats, NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY, 10.1007/s00210-015-1171-9, 388, 10, 1113-1117, 2015.10.
57. Takashi Ichinose, Kazuki Moriyasu, Akane Nakahara, Mitsuru Tanaka, Matsui Toshiro, Shigeki Furuya, Orally administrated dipeptide Ser-Tyr efficiently stimulates noradrenergic turnover in the mouse brain, BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 10.1080/09168451.2015.1044932, 79, 9, 1542-1547, 2015.09.
58. Mitsuru Tanaka, Seong-Min Hong, Sayaka Akiyama, Qing-Qiang Hu, Matsui Toshiro, Visualized absorption of anti-atherosclerotic dipeptide, Trp-His, in Sprague-Dawley rats by LC-MS and MALDI-MS imaging analyses, MOLECULAR NUTRITION & FOOD RESEARCH, 10.1002/mnfr.201500075, 59, 8, 1541-1549, 2015.08.
59. Ruge Cao, Yutaro Kobayashi, Airi Nonaka, Yuji Miyata, Kazunari Tanaka, Takashi Tanaka, Matsui Toshiro, NMR Spectroscopic and Quantum Mechanical Analyses of Enhanced Solubilization of Hesperidin by Theasinensin A, PHARMACEUTICAL RESEARCH, 10.1007/s11095-015-1621-6, 32, 7, 2301-2309, 2015.07.
60. Ruge Cao, Airi Nonaka, Fusae Komura, Matsui Toshiro, Application of diffusion ordered-H-1-nuclear magnetic resonance spectroscopy to quantify sucrose in beverages, FOOD CHEMISTRY, 10.1016/j.foodchem.2014.08.105, 171, 8-12, 2015.03.
61. Si Jing Chen, Chiwa Aikawa, Toshiro Matsui, Quantitative analysis of methylglyoxal, glyoxal and free advanced glycation end-products in the plasma of wistar rats during the oral glucose tolerance test, Biological and Pharmaceutical Bulletin, 10.1248/bpb.b14-00698, 38, 2, 336-339, 2015.02, The purpose of this study was to gain insight into the production behavior of free adducts of advanced glycation end-products (AGEs) in Wistar rats under acute hyperglycemic conditions. Five AGE-free adducts as well as their precursors (i.e., highly reactive carbonyl intermediates of methylglyoxal and glyoxal) in rat plasma were quantitatively determined at greater than nanomolar levels using the liquid chromatography/ tandem mass spectrometry method coupled with 2,4,6-trinitrobenzene sulfonate and 2,3-diaminonaphthalene derivatization techniques. An oral glucose (2 g/kg dose) tolerance test to 10-week-old Wistar rats provided evidence that the plasma levels of diabetes-related metabolites did not change acutely within 120 min, irrespective of increasing blood glucose levels..
62. Ha-Young Park, Yuri Kunitake, Naoto Hirasaki, Mitsuru Tanaka, Matsui Toshiro, Theaflavins enhance intestinal barrier of Caco-2 Cell monolayers through the expression of AMP-activated protein kinase-mediated Occludin, Claudin-1, and ZO-1, BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 10.1080/09168451.2014.951027, 79, 1, 130-137, 2015.01.
63. Jiang Guo, Tomomi Saiki, Kumrungsee Thanutchaporn, Wanying Liu, Akihiro Shimura, Matsui Toshiro, Elution Profile of Di-peptides on a Sulfonated Ethylstyrene-Divinylbenzene Copolymer Resin Column by High-performance Liquid Chromatography, ANALYTICAL SCIENCES, 31, 1, 45-50, 2015.01.
64. Si Jing Chen, Chiwa Aikawa, Risa Yoshida, Toshiro Matsui, Methylglyoxal-derived hydroimidazolone residue of plasma protein can behave as a predictor of prediabetes in Spontaneously Diabetic Torii rats, Physiological Reports, 10.14814/phy2.12477, 3, 8, 2015.08, Prediabetes, typically defined as impaired glucose tolerance and/or impaired fasting blood glucose, is a high-risk state of developing diabetes. The association of diabetes-related metabolites with prediabetes has not been investigated intensively. This study aimed to get insights into the metabolic behaviors of some typical diabetes-related metabolites in plasma of male Spontaneously Diabetic Torii (SDT) rats during pathogenic progress of diabetes and to assess in vivo if the variation in these metabolites related to the progression of prediabetic stage. To address this question, SDT rats and Sprague Dawley (SD) rats as control were maintained from the age of 7 to 25 weeks. Five typical advanced glycation end products (AGEs) residue of plasma protein and their free adducts were determined by liquid chromatography with tandem mass detection over the duration of the investigation. The SDT rats exhibited impaired glucose tolerance since the age of 12 weeks and developed diabetes with significantly elevated fasting glucose levels after 22 weeks. At the prediabetic stage (12–21 weeks), no significant differences were observed on AGEfree adducts levels of SDT rats compared with SD rats. However, methylglyoxal- derived hydroimidazolone (MG-H1) residue contents of plasma protein were significantly elevated in SDT rats at the age of 16 weeks, whereas other AGE residues of plasma protein did not show marked difference. The present study has revealed significant increase in MG-H1 residue content of plasma protein at the prediabetic stage of a spontaneously diabetic rat model, irrespective of unaltered fasting blood glucose and constant plasma levels of other AGEs..
65. Mitsuru Tanaka, Eiji Tanaka, Noe Obinata, Toshiro Matsui, Visualization of tocopherol acetate absorbed inside laminated films by a matrix-assisted laser desorption/ionization-imaging mass spectrometry, Food Science and Technology Research, 10.3136/fstr.21.821, 21, 6, 821-826, 2015.12, The aim of this study was to visualize the sorption of tocopherol acetate as a penetrant into polymer films. A matrix-assisted laser desorption/ionization-imaging mass spectrometry (MALDI-IMS) technique was used to visualize the distribution of tocopherol acetate in films during the migration process. A phytic acid-aided MALDIIMS using 2',4',6'-trihydroxyacetophenone as a matrix allowed us to successfully visualize the absorbed tocopherol acetate in both ethylene-vinyl acetate copolymer (EVA) and low-density polyethylene (LDPE) films after 1 weekstorage of tocopherol acetate at 40°C. The IMS technique allowed us to also visualize the actual distribution of tocopherol acetate inside the EVA/barrier/EVA laminated films, in which tocopherol acetate sorption was blocked at the barrier layer..
66. Kumrungsee Thanutchaporn, Saiki Tomomi, Akiyama Sayaka, Nakashima Kentaro, Mitsuru Tanaka, Kobayashi Yutaro, Matsui Toshiro, Inhibition of calcium-calmodulin complex formation by vasorelaxant basic dipeptides demonstrated by in vitro and in silico analyses, BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS, 10.1016/j.bbagen.2014.07.020, 1840, 10, 3073-3078, 2014.10.
67. Kovacs-Nolan Jennifer, Rupa Prithy, Matsui Toshiro, Mitsuru Tanaka, Konishi Toni, Sauchi Yusuke, Sato Kenji, Ono Shin, Mine Yoshinori, In Vitro and ex Vivo Uptake of Glutathione (GSH) across the Intestinal Epithelium and Fate of Oral GSH after in Vivo Supplementation, JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 10.1021/jf503257w, 62, 39, 9499-9506, 2014.10.
68. Zhao Jian, Suyama Aki, Mitsuru Tanaka, Matsui Toshiro, Ferulic acid enhances the vasorelaxant effect of epigallocatechin gallate in tumor necrosis factor-alpha-induced inflammatory rat aorta, JOURNAL OF NUTRITIONAL BIOCHEMISTRY, 10.1016/j.jnutbio.2014.03.013, 25, 7, 807-814, 2014.07.
69. Shimamura, Tomoko, Sumikura Yoshihiro, Yamazaki Takeshi, Tada Atsuko, Kashiwagi Takehiro, Ishikawa Hiroya, Matsui Toshiro, Sugimoto Naoki, Akiyama Hiroshi, Ukeda Hiroyuki, Applicability of the DPPH Assay for Evaluating the Antioxidant Capacity of Food Additives - Inter-laboratory Evaluation Study, ANALYTICAL SCIENCES, 30, 7, 717-721, 2014.07.
70. Kumrungsee Thanutchaporn, Wang Zheng-Quan, Matsumura Shinya, Saiki Tomomi, Mitsuru Tanaka, Matsui Toshiro, Identification of peptides from soybean protein, glycinin, possessing suppression of intracellular Ca2+ concentration in vascular smooth muscle cells, FOOD CHEMISTRY, 10.1016/j.foodchem.2013.11.143, 152, 218-224, 2014.06.
71. Cao Ruge, Komura Fusae, Nonaka Airi, Kato Takeshi, Fukumashi Junji, Matsui Toshiro, Quantitative analysis of D-(+)-glucose in fruit juices using diffusion ordered-1H nuclear magnetic resonance spectroscopy, Analytical Sciences, 30, 3, 383-388, 2014.03.
72. Fukuda Toshihiko, Kuroda Takahiro, Kono Miki, Takahisa Miyamoto, Mitsuru Tanaka, Matsui Toshiro, Attenuation of L-Type Ca2+ Channel Expression and Vasomotor Response in the Aorta with Age in Both Wistar-Kyoto and Spontaneously Hypertensive Rats, PLOS ONE, 10.1371/journal.pone.0088975, 9, 2, e88975-e88983, 2014.02.
73. Qiu Ju, Maekawa Kanako, Kitamura Yuko, Miyata Yuji, Tanaka Kazunari, Tanaka Takashi, Soga Minoru, Tsuda Takanori, Matsui Toshiro, Stimulation of glucose uptake by theasinensins through the AMP-activated protein kinase pathway in rat skeletal muscle cells, BIOCHEMICAL PHARMACOLOGY, 10.1016/j.bcp.2013.10.029, 87, 2, 344-351, 2014.01.
74. Seong-Min Hong, Mitsuru Tanaka, Saori Yoshii, Yoshinori Mine, Matsui Toshiro, Enhanced visualization of small peptides absorbed in rat small intestine by phytic-acid-aided MALDI-imaging mass spectrometry, Anal. Chem., 85, 11, 10033-10039, 2013.11.
75. Takeda, Junko, Park, Ha-Young, Kunitake, Yuri, Yoshiura, Keiko, Matsui Toshiro, Theaflavins, dimeric catechins, inhibit peptide transport across Caco-2 cell monolayers via down-regulation of AMP-activated protein kinase-mediated peptide transporter PEPT1, FOOD CHEMISTRY, 10.1016/j.foodchem.2012.12.026, 138, 4, 2140-2145, 2013.06.
76. Hoang Lan Phuong, Qiu, Ju, Matsui Toshiro, Kuwahara, Takashi, Fukui, Keiichi, Yoshiyama, Kayo, Matsugano, Kazusato, Terahara, Norihiko, Transepithelial transport of 6-O-caffeoylsophorose across Caco-2 cell monolayers, FOOD CHEMISTRY, 10.1016/j.foodchem.2012.10.137, 138, 1, 9048-9054, 2013.05.
77. Chihiro Hashimoto, Yuri Iwaihara, Si-Jing Chen, Tanaka Mitsuru, Toshiaki Watanabe, Matsui Toshiro, Highly-Sensitive Detection of Free Advanced Glycation End-Products by Liquid Chromatography-Electrospray Ionization-Tandem Mass Spectrometry with 2,4,6-Trinitrobenzene Sulfonate Derivatization, ANALYTICAL CHEMISTRY, 10.1021/ac400294q, 85, 9, 4289-4295, 2013.05, 生体内での糖化産物である各種AGEを高感度分析する新たなアッセイ法(LC-MS/MS-MRM-TNBS法)を提案した。.
78. Ha-Young Park, Yuri Kunitake, Matsui Toshiro, Benzotropolone moiety in theaflavins is responsible for inhibiting peptide-transport and activating AMP-activated protein kinase in Caco-2 cells., J. Functional Foods in Health and Disease, 3, 5, 111-121, 2013.05.
79. Mahmud Tareq Hassan Khan, Kenichi Dedachi, Matsui Toshiro, Noriyuki Kurita, Monica Borgatti, Ingebrigt Sylte, Dipeptide inhibitors of thermolysin and angiotensin I-converting enzyme., Current Topics in Medicinal Chemistry, 12, 16, 1748-1762, 2012.10.
80. Jennifer Kovacs-Nolan, Hua Zhang, Masahisa Ibuki, Toshihiro Nakamori, Keiko Yoshiura, Patricia V Turner, Matsui Toshiro, Yoshinori Mine, The PepT1-transportable soy tripeptide VPY reduces intestinal inflammation, Biochim. Biophys. Acta-General Subjects, 1820, 1753-1763, 2012.06.
81. Eui-Baek Byun, Teruaki Ishikawa, Aki Suyama, Masaya Kono, Shohei Nakashima, Tomomasa Kanda, Miyamoto Takahisa, Matsui Toshiro, A procyanidin trimer, C1, promotes NO production in rat aortic endothelial cells via both hyperpolarization and PI3K/Akt pathways, Eur. J. Pharm, 10.1016/j.ejphar.2012.07.011, 692, 52-60, 2012.06.
82. Yutaro Kobayashi, T.Fukuda, Mitsuru Tanaka, Matsui Toshiro, The anti-atherosclerotic di-peptide, Trp-His, inhibits the phosphorylation of voltage-dependent L-type Ca2+ channels in rat vascular smooth muscle cells, FEBS Open Bio, 10.1016/j.fob.2012.04.005, 2, 83-88, 2012.04.
83. Shinji Tokuda, Toshihiko Fukuda, Yutaro Kobayashi, Mitsuru Tanaka, Toshiro Matsui, Effect of the uncharged imidazolium moiety in adenine on endothelium-independent relaxation in the contracted thoracic aorta of sprague-dawley rats, Bioscience, Biotechnology and Biochemistry, 10.1271/bbb.110806, 76, 4, 828-830, 2012.04, Adenine had a concentration-dependent relaxation action on the phenylephrine-contracted aorta ring, with an EC50 value of 0:40 ± 0:12mM. This effect was also observed in the endothelium-denuded aorta. Among the adenine analogues, N-methyladenine and benzimidazole still evoked an apparent relaxation effect, while 1-, 3- or 7-methyladenine and imidazole were no longer vasodilators. These findings demonstrate that the imino group from the uncharged imidazolium moiety in adenine played a key role in the relaxation of the contracted aorta..
84. Yuji Miyata, Takashi Tanaka, Kei Tamaya, Toshiro Matsui, Shizuka Tamaru and Kazunari Tanaka, Cholesterol-lowering effect of black tea polyphenols, theaflavins, Theasinensin A and thearubigins, in rats fed high fat diet, Food Sci. Technol. Res., 17, 6, 583-588, 2011.12.
85. Takashi Hasegawa, Kiyoshi Matsumoto, Toshiro Matsui, Effect of multistratification and film depth on the visualized dynamic sorption behavior of perylene in cellulose acetate films by a confocal laser scanning microscope technique, Journal of Applied Polymer Science, 10.1002/app.33322, 121, 3, 1780-1787, 2011.08, The sorption behavior of a fluorescent reagent into a polymer film was visualized by confocal laser scanning microscopy (CLSM), and the effects of the additives, film types, and film depth on the diffusion coefficient (D) of the fluorescence reagent were examined. Perylene and cellulose acetate (CA) were used as a fluorescent reagent and a polymer material, respectively. Perylene dissolved in the additives triethylene glycol diacetate (TEGDA) and glycerol triacetate (GTA) was added to the CA film. Then, the evaluation of two types of CA films, a closed-system cellulose acetate (CCA) sample and an open-system cellulose acetate (OCA) sample, was conducted. At optimized CLSM conditions (with a scanning range at a 20-lm depth from the CA film surface with 1-μm intervals and a scanning speed of 1 fps), the sorption of perylene at the inner CA film was determined. The D values of perylene in the CA film were calculated pursuant to Fick's second law. Higher D values of perylene mixed with TEGDA versus those of perylene mixed with GTA were commonly obtained for the CCA sample (TEGDA: 8.9 × 10-15 m 2/s > GTA: 1.7 × 10-15 m2/s) and the OCA sample (TEGDA: 11 × 10-15 m2/s > GTA: 3.3 × 10-15 m 2/s) because of the higher chemical affinity of TEGDA with perylene than that of GTA. Perylene indicated a higher D value and was homogeneously distributed in the case of the OCA sample; we found that diffusivity and distribution of perylene in CA film were largely affected by the multistratification treatment. We also proved that the deeper the film depth was, the lower the diffusivity of perylene was, regardless of the types of additives and films. The factors considered for the film-depth dependence of D were a gradual increase in the diffusion pathway for perylene caused by additive diffusion and the concentration dependence of the perylene D..
86. E. M. N. Nakashima, A.Kudo, Y. Iwaihara, M. Tanaka, K. Matsumoto, T. Matsui, Application of 13C-stable-isotope labeling LC-MRM-MS/MS method for determining intact absorption of bioactive di-peptides in rats., Anal. Biochem., 10.1016/j.ab.2011.02.037, 414, 4, 109-116, 2011.04.
87. N. Inoue, K. Nagao, K.Sakata, N.Yamano, P.E.R.Gunawardena, S.Y.Han, T.Matsui, T.Nakamori, H.Furuta, K.Takamatsu and T.Yanagita, Screening of soy protein-derived hypotriglyceridemic di-peptides in vitro and in vivo, Lipids in Health and Disease, 10, 85-94, 2011.04.
88. Toshiro Matsui, Akihiro Kudo, Shinji Tokuda, Kiyoshi Matsumoto, Hirokazu Hosoyama, Identification of a new natural vasorelaxatant compound, (+)-osbeckic acid, from rutin-free tartary buckwheat extract, Journal of Agricultural and Food Chemistry, 10.1021/jf1028416, 58, 20, 10876-10879, 2010.10, The candidates responsible for vasorelaxation action of rutin-free tartary buckwheat extract (TBSP) were examined in this study. As a result of reversed-phase high-performance liquid chromatography (HPLC) separations, five prominent peaks in the acidic fraction of TBSP were obtained at 260 nm. Among the five collected peaks, we successfully identified four compounds by nuclear magnetic resonance (NMR) and mass spectrometry (MS) measurements: (+)-osbeckic acid as a dimer ([M - H]- m/z: 371.2 > 184.9 > 140.9), 5-hydroxymethyl-2-furoic acid, protocatechuic acid, and p-hydroxybenzoic acid. A vascular contractive measurement in 1.0 μM phenylephrine-contracted Sprague-Dawley rat thoracic aorta rings revealed that (+)-osbeckic acid dimer evoked a potent vasorelaxant effect with an EC50 value of 887 μM compared to other isolates (EC50: 5-hydroxymethyl-2-furoic acid, 3610 μM; protocatechuic acid, 2160 μM; p-hydroxybenzoic acid, no inhibition). Dimeric (+)-osbeckic acid was stable in solutions and at high temperatures, while its degraded peak on the HPLC chromatogram was observed when it was dissolved in dimethyl sulfoxide..
89. Asami Toshima, Toshiro Matsui, Mai Noguchi, Ju Qiu, Kei Tamaya, Yuji Miyata, Takashi Tanaka, Kazunari Tanaka, Identification of α-glucosidase inhibitors from a new fermented tea obtained by tea-rolling processing of loquat (Eriobotrya japonica) and green tea leaves, Journal of the Science of Food and Agriculture, 10.1002/jsfa.3983, 90, 9, 1545-1550, 2010.07, Background: A new fermented tea produced by tea-rolling processing of loquat (Eriobotrya japonica) leaf with green tea leaf (denoted as LG tea) showed a potent antihyperglycaemic effect in maltose-loaded rats. The aim of this study, therefore, was to identify α-glucosidase inhibitors in the antihyperglycaemic tea product. Results: LG tea had a threefold higher maltase-inhibitory activity (IC50 0.065 mgdried extractm L-1) than either the constituent loquat leaf or green tea alone. In addition, LG tea favourably inhibited maltase action rather than sucrase action. As a result of bioguided high-performance liquid chromatography separations of LG tea, theasinensin A, theasinensin B, strictinin and 1,6-digalloylglucose were newly identified asmaltase inhibitors with IC50 values of 142, 225, 398 and 337 μmol L-1 respectively, along with previously identified catechins and theaflavins. Conclusion: Judging from the magnitude of the α-glucosidase-inhibitory contribution of each isolated compound to the overall inhibition of LG tea, catechins were the main candidates responsible for α-glucosidase or maltase inhibition in LG tea, followed by theaflavins, theasinensins, strictinin and 1,6-digalloylglucose..
90. Toshiro Matsui, Xiao Lin Zhu, Koso Shiraishi, Tatsuro Ueki, Yoshiharu Noda, Kiyoshi Matsumoto, Antihypertensive effect of salt-free soy sauce, a new fermented seasoning, in spontaneously hypertensive rats, Journal of Food Science, 10.1111/j.1750-3841.2010.01599.x, 75, 4, 2010.05, In this study, we focused on the antihypertensive effect of newly fermented salt-free soy sauce (SFS) in spontaneously hypertensive rats (SHRs). A 13-wk oral administration of SFS was performed daily in 8- to 21-wk-old SHRs with a dose of 200 mg/kg of body weight/d. The systolic (SBP) and diastolic (DBP) blood pressures were measured for each week during the experimental period. As a result, a significant (P 21-wk: 27 mmHg, ΔDBP21-wk: 20 mmHg). In contrast to such substantial effect by SFS intake, angiotensin I-converting enzyme activities in blood and local organs as well as pharmaceutical parameters such as serum Na+ or K+ level did not show any significant difference between in both SFS and control groups. Vasoconstriction experiment using thoracic aorta rings from 21-wk SHRs provided an interesting result that the rings from the SFS group evoked a >2-fold higher increase in the angiotensin II-stimulated constrictive response compared with the rings from the control group (P = 0.012), which suggested that the SFS-intake would be effective in possessing a higher vessel tone.Practical Application: In this study, we investigated the antihypertensive effect of newly fermented SFS in SHRs. As a result of 13-wk SFS-administration, we found a significant antihypertensive effect of the SFS. This finding strongly demonstrates that the developed SFS would be greatly beneficial for health and useful for health-related industries..
91. Takashi Hasegawa, Toshiro Matsui, Kiyoshi Matsumoto, Application of confocal laser scanning microscope technique to visualize dynamic sorption behavior of perylene in cellulose acetate film, Journal of Applied Polymer Science, 10.1002/app.31392, 116, 3, 1552-1557, 2010.05, Additives distributed in a polymer film were visualized by a confocal laser scanning microscope (CLSM) in this study. Cellulose acetate (CA) was used as the polymer film material. Perylene as a fluorescence reagent and glycerol triacetate (GTA) as a plasticizer for CA, respectively, were selected and mixed for visualizing the absorption of perylene into CA. Under optimized CLSM conditions of an objective lens (dry, 20×) and diode laser (408 nm, 290-330 μW), a fluorescent penetrant, perylene, in GTA solution was detected in CA casting film within around 9 μm depth of the film in 60 minutes after starting the sorption study. It was also found that the fluorescence intensity of perylene became lower with the film depth. This finding suggested that CLSM could visualize the sorption behavior of perylene as a dynamic diffusion process. Analytical conditions, such as a scanning range along the Z axis (±10 or ±30 μm from an air-contact surface of the CA film) and a scan speed (1 or 30 fps) of CLSM, did not affect the fluorescence intensity of the sorbed perylene. It was concluded that a newly developed analytical methodology using CLSM was sufficient for visualizing the perylene penetrant across the cross-sectional distribution of the CA film, being capable of monitoring the sorption behavior of compounds in polymer materials without any destruction of a given absorbed film..
92. Takashi Hasegawa, Toshiro Matsui, Kiyoshi Matsumoto, Comparative study of additives on visualized dynamic sorption behavior of perylene in cellulose acetate film by confocal laser scanning microscope technique, Journal of Applied Polymer Science, 10.1002/app.31607, 116, 3, 1710-1717, 2010.05, The sorption behavior of a fluorescent reagent into a polymer film was visualized by confocal laser scanning microscope (CLSM), and the effect of additives on the sorption was examined. Perylene and cellulose acetate (CA) were used as a fluorescent reagent and a polymer material, respectively. Perylene dissolved in additives of glyc-erol triacetate (GTA), triethylene glycol diacetate (TEGDA), 1,3-butylene glycol diacetate (BGDA), paraffin liquid, and poly(ethylene glycol) 200 (PEG) were added onto CA film. At optimized CLSM conditions (scanning range of a 10 lm-depth from the CA film surface with 1 lm intervals and a scanning speed of 1 fps), the sorption of perylene at the inner CA film was determined. Diffusion coefficients of perylene in the CA film were calculated according to Fick's second law. When perylene was dissolved in TEGDA, the highest diffusion coefficient, 8.9 × 10-15 m 2/s, among the additives was obtained because of high affinity of TEGDA with perylene, whereas the lowest diffusion coefficient was observed in paraffin liquid, which showed a low affinity with perylene. The diffusion coefficients of perylene in GTA, BGDA, and PEG also revealed that physical parameters, such as molecular size and viscosity, of the additives were additional factors affecting the perylene diffusivity in the CA film..
93. Roman Selyanchyn, Serhiy Korposh, Toshiro Matsui, Hidetaka Matsui, Seung Woo Lee, Purge and trap sampling coupled to curie point thermal desorption for the detection of parts per trillion 2,4,6-trichloroanisole in water, Chromatographia, 10.1365/s10337-009-1428-x, 71, 3-4, 317-321, 2010.02, GC-MS analysis based on purge and trap sampling followed by Curie point thermal desorption was examined for the quantitative determination of 2,4,6-trichloroanisole in aqueous solutions. The method developed showed linearity in single ion monitoring mode over a TCA concentration range of 0.12-120 ng L-1 (parts-per-trillion)..
94. Toshiro Matsui, Sachiko Korematsu, Eui-Baek Byun, Taichi Nishizuka, Shunji Ohshima and Tomomasa Kanda, Apple Procyanidins Induce Vascular Relaxation in Isolated Rat Aorta through NO/cGMP Pathway in Combination with Hyperpolarization by Multiple K+ Channel Activations, Biosci. Biotechnol. Biochem., 73(10), 2246-2251, 2009.10.
95. Norihiko Terahara, Toshiro Matsui, Kanako Minoda, Kanae Nasu, Risa Kikuchi, Keiichi Fukui, O. N.O. Hiroshi, Kiyoshi Matsumoto, Functional new acylated sophoroses and deglucosylated anthocyanins in a fermented red vinegar, Journal of Agricultural and Food Chemistry, 10.1021/jf901809p, 57, 18, 8331-8338, 2009.09, The new acylated polyphenols were isolated from a red-colored vinegar produced via fermentation with purple-fleshed sweet potato storage roots, and identified mainly by MS and NMR. The three acylated sophoroses were determined as 6-O-(E)-caffeoyl-(2-O-(6-O-acyl)-β-D-glucopyranosyl)-D-glucopyranoses, where acyl was (E)-caffeoyl, p-hydroxybenzoyl, and (E)-feruloyl, respectively. The four acylated anthocyanins were also determined as cyanidin 3-O-(6-O-(E)-caffeoyl-(2-O-(6-O-(E)-feruloyl)β-D-glucopyranosyl) -β-D-glucopyranoside), in addition to peonidin 3-O-(6-O-(E)-caffeoyl-(2-O- (6-O-acyl)D-glucopyranosyl)-β-D-glucopyranosides), where acyl was (E)-caffeoyl, p-hydroxybenzoyl, and (£)-feruloyl, respectively. The diacylated sophoroses showed higher antioxidant capacity than that of monoacylated analogue 6-caffeoylsophorose, so the multiacylation established to enhance their antioxidant capacity. Similarly, 5-deglucosylated anthocyanins also gave somewhat stronger antioxidation than corresponding sweet potato anthocyanins. In rat intestinal α-glucosidase inhibition study, the diacylated sophoroses preferably inhibited maltase rather than sucrase with an IC50 value of
96. Mitsuru Tanaka, Shimpei Watanabe, Zhengquan Wang, Kiyoshi Matsumoto, Toshiro Matsui, His-Arg-Trp potently attenuates contracted tension of thoracic aorta of Sprague-Dawley rats through the suppression of extracellular Ca2+ influx, Peptides, 10.1016/j.peptides.2009.05.012, 30, 8, 1502-1507, 2009.08, In the present study, we primarily attempted to identify di- and tri-peptides showing potent vasodilation in 1.0 μM phenylephrine-contracted thoracic aortas of Sprague-Dawley rats. Synthetic 15 Trp-His (WH) skeleton analogues were used for rat aorta ring's force measurements, since WH was found to be a vasoactive di-peptide so far. Among the synthesized peptides consisted of both His and Trp amino acid residues, His-Arg-Trp (HRW) was found to evoke the most potent vasodilation with an EC50 value of 1.2 ± 0.08 mM in an endothelium-independent manner, while no effect was evoked by a mixture of individual amino acids. In addition to the structure of tri-peptides-activity relationship, chemically modified HRW analogues, i.e., 1- or 3-methyl-His-Arg-Trp and His-citrulline-Trp demonstrated the structural importance of tri-peptide to evoke the vasoactivity as following factors: (1) Neutral imidazole and indole groups from His and Trp residues at N- and C-terminals, respectively and (2) basic amino acids at the middle position. In mitogen (10 μM angiotensin II or 50 μM Bay K8644)-stimulated vascular smooth muscle cells, vasoactive HRW (100 μM) caused significant [Ca2+]i reduction to an extent of >30%. Thus, our results suggest that HRW caused vasodilation action via an endothelium-independent mechanism which probably involves the suppression of extracellular Ca2+ influx through voltage-gated l-type Ca2+ channel..
97. Takashi Tanaka, Yuji Miyata, Kei Tamaya, Rie Kusano, Yosuke Matsuo, Shizuka Tamaru, Kazunari Tanaka, Toshiro Matsui, Masamichi Maeda, Isao Kouno, Increase of theaflavin gallates and thearubigins by acceleration of catechin oxidation in a new fermented tea product obtained by the tea-rolling processing of loquat (Eriobotrya Japonica) and green tea leaves, Journal of Agricultural and Food Chemistry, 10.1021/jf900963p, 57, 13, 5816-5822, 2009.07, In a project to produce a new fermented tea product from non-used tea leaves harvested In the summer, we found that kneading tea leaves (Camellia sinensis) with fresh loquat leaves (Eriobotrya japonica) accelerated the enzymatic oxidation of tea catechins. The fermented tea obtained by tea-rolling processing of tea and loquat leaves had a strong, distinctive flavor and a plain aftertaste, which differed from usual black, green, and oolong teas. The phenolic constituents were similar to those of black tea. However, the concentrations of theaflavin 3-O-gallate, theaflavin 3,3′-di-O-gallate, and thearubigins were higher in the tea leaves kneaded with loquat leaves than in tea leaves kneaded without loquat leaves. The results from in vitro experiments suggested that acceleration of catechin oxidation was caused by the strong oxidation activity of loquat leaf enzymes and a coupled oxidation mechanism with caffeoyl quinic acids, which are the major phenolic constituents of loquat leaves..
98. Tomoko Fujise, Norihiko Terahara, Keiichi Fukui, Koichi Sugita, Hideaki Ohta, Toshiro Matsui, Kiyoshi Matsumoto, Durable antihyperglycemic effect of 6-O-caffeoylsophorose with α-glucosidase inhibitory activity in rats., Food Sci. Technol. Res., 14(5), 477-484, 2008.10.
99. Xiao Lin Zhu, Keisuke Watanabe, Koso Shiraishi, Tatsuro Ueki, Yoshiharu Noda, Toshiro Matsui, Kiyoshi Matsumoto, Identification of ACE-inhibitory peptides in salt-free soy sauce that are transportable across caco-2 cell monolayers, Peptides, 10.1016/j.peptides.2007.11.006, 29, 3, 338-344, 2008.03, In present study, we aimed to identify angiotensin I-converting enzyme (ACE)-inhibitory peptides from a salt-free soy sauce (SFS), a newly developed antihypertensive seasoning obtained by Aspergillus oryzae fermentation of soybean in the absence of salt, which can be transported through caco-2 cell monolayers. Through an Ussing transport investigation of SFS across caco-2 cell monolayers, three di-peptides, Ala-Phe, Phe-Ile and Ile-Phe, were successfully identified from the SFS as transportable inhibitory peptides. Ala-Phe and Ile-Phe, but not Phe-Ile, exhibited ACE-inhibitory activity with IC50 values of 165.3 μM and 65.8 μM, respectively. Kinetic studies revealed that Ile-Phe (Km: 3.1 mM, Papp: 2.4 × 10-6 cm/s) exhibited greater affinity toward the transport compared with Ala-Phe (Km: 48.1 mM, Papp: 1.4 × 10-6 cm/s) and Phe-Ile (Km: 12.7 mM, Papp: 1.4 × 10-6 cm/s)..
100. Toshiro Matsui, Takashi Tanaka, Satomi Tamura, Asami Toshima, Kei Tamaya, Yuji Miyata, Kazunari Tanaka, Kiyoshi Matsumoto, α-glucosidase inhibitory profile of catechins and theaflavins, Journal of Agricultural and Food Chemistry, 10.1021/jf0627672, 55, 1, 99-105, 2007.01, To clarify the postprandial glucose suppression effect of flavonoids, the inhibitory effects of catechins and theaflavins against α-glucosidase (AGH) were examined in this study. It was initially demonstrated that theaflavins and catechins preferentially inhibited maltase rather than sucrase in an immobilized AGH inhibitory assay system. For the maltase inhibitory effects of theaflavins, the effects were observed in descending order of potency of theaflavin (TF)-3-O-gallate (Gal) > TF-3,3′-di-O-Gal > TF-3′-O-Gal > TF. This suggests that the AGH inhibition induced by theaflavins is closely associated with the presence of a free hydroxyl group at the 3′-position of TF as well as the esterification of TF with a mono-Gal group. In addition, the R-configuration at the 3′-position of TF-3-O-Gal showed a higher inhibitory activity than the S-configuration. As a result of a single oral administration of maltose (2 g/kg) in rats, a significant reduction in blood glucose level was observed at a dose of 10 mg/kg of TF-3-O-Gal, demonstrating for the first time that TF-3-O-Gal can suppress glucose production from maltose through inhibition of AGH in the gut..
101. Toshiro Matsui, Isiaka Ogunwande, Kanthi J. M. Abesundara, and Kiyoshi Matsumoto, Anti-hyperglycemic Potential of Natural Products, Mini-Reviews in Medicinal Chemistry, 6(3), 109-120, 2006.02.
102. T.Matsui, T.Ueno, M.Tanaka, H.Oka, T.Miyamoto, K.Osajima, K.Matsumoto, Antiproliferative action of an angiotensin I-converting enzyme inhibitory peptide, Val-Tyr, via an L-type Ca2+ channel inhibition in cultured vascular smooth muscle cells, Hypertension Res., 10.1291/hypres.28.545, 28, 6, 545-552, 28(6), 545-552, 2005.06.
103. T.Matsui, S.Ebuchi, T.Fujise, K.J.M.Abesundara, S.Doi, H.Yamada, K.Matsumoto, Strong antihyperglycemic effects of water-soluble fraction of Brazilian propolis and its bioactive constituent, 3, 4, 5-tri-O-caffeoylquinic acid, Biol. Pharm. Bull., 10.1248/bpb.27.1797, 27, 11, 1797-1803, 27, 1797-1803, 2004.11.
104. K.Abesundara, T.Matsui, K.Matsumoto, a-Glucosidase inhibitory activity of some Sri Lanka plant extracts, One of which, Cassia auriculata, exerts a strong antihyperglycemic effect in rats comparable to the therapeutic drug acarbose, J.Agric. Food Chem., 10.1021/jf035330s, 52, 9, 2541-2545, 52, 2541-2544, 2004.05.
105. T.Matsui, M.Imamura, H.Oka, K.Osajima, K.Kimoto, T.Kawasaki, K.Matsumoto, Tissue distribution of antihypertensive dipeptide, Val-Tyr, after its single oral administration to spontaneously hypertensive rats, J.Peptide Sci., 10.1002/psc.568, 10, 9, 535-545, 10,535-545, 2004.01.
106. T.Matsui, A.Hayashi, K.Tamaya, K.Matsumoto, T.Kawasaki, K.Murakami, and K.Kimoto, Depressor effect induced by dipeptide, Val-Tyr, in hypertensive transgenic mice is due, in part, to the suppression of human circulating renin-angiotensin system, Clin. Exp. Pharmacol. Physiol., 10.1046/j.1440-1681.2003.03824.x, 30, 4, 262-265, 30(4), 262-265, 2003.04.
107. N.Terahara, T.Matsui, K.Fukui, K.Matsugano, K.Sugita, and K.Matsumoto, Caffeoylsophorose in a red vinegar produced through fermentation with purple sweetpotato, J. Agric. Food Chem., 10.1021/jf026146u, 51, 9, 2539-2543, 51(9), 2539-2543, 2003.03.
108. K.Nagao, N.Inoue, Y.-M.Wang. J.Hirata, Y.Shimada, T.Nagao, T.Matsui, and T.Yanagita, The 10trans,12cis isomer of conjugated linoleic acid suppress the development of hypertension in Otsuka Long-Evans Tokushima fatty rats, Biochem. Biophys. Res. Commun., 10.1016/S0006-291X(03)00929-X, 306, 1, 134-138, 306, 134-138, 2003.05.
109. SOD-like activity of sardine muscle hydrolysate by alkaline protease..
110. Antihypertensive effect of food containing sardine peptides in high-normal hypertensives amd hypertensives..
111. T. Oki, T. Matsui, K. Matsumoto, Evaluation of α-glucosidase inhibition by using an immobilized assay system, Biological and Pharmaceutical Bulletin, 23, 9, 1084-1087, 2000.09, The inhibitory effects of natural and synthetic inhibitors on the intestinal membrane-bound hydrolase, α-glucosidase (AGH), were evaluated by using an immobilized cyanogen bromide-activated Sepharose 4B support. Immobilized AGH (iAGH) inhibition study by synthetic inhibitors (acarbose and voglibose) revealed that the magnitude of inhibition differed from that in the free AGH (fAGH) study: IC50 value of acarbose in iAGH-maltase assay system, 340 - 430 nM; fAGH, 11 nM. iAGH-maltase inhibition by both inhibitors was influenced by blocking reagents with different functional groups (COOH, OH, CH3, and NH2 groups). On the other hand, significant iAGH-sucrase inhibitory activity was observed only when using the negatively charged support induced by 0.1 M β-alanine. The K(m) values obtained in the iAGH assay system were similar to those from the fAGH method. With natural inhibitors, the iAGH-sucrase inhibitory activity of D-xylose, with in vivo glucose suppression, increased twice compared to that in fAGH. Green tea extract gave almost the same inhibition for both AGH assay systems..
112. T.Matsui, K.Tamaya, E.Seki, K.Osajima, K.Matsumoto, and T.Kawasaki, Absorption of Val-Tyr with in vitro angiotensin I-converting enzyme inhibitory activity into the circulating blood system of mild hypertensive subjects., Biol. Pharmacol. Bull., 10.1248/bpb.25.1228, 25, 9, 1228-1230, 25(9), 1228-1230, 2002.09.
113. T.Matsui, S.Ebuchi, M.Kobayashi, K.Fukui, K.Sugita, N.Terahara, and K.Matsumoto, Anti-hyperglycemic effect of diacylated anthocyanin derived from Ipomoea batatas cv. Ayamurasaki can be achieved through the a-glucosidase inhibitory action, J. Agric. Food Chem., 10.1021/jf025913m, 50, 25, 7244-7248, 50(25),7244-7248, 2002.12.
114. K.Matsumoto, K.Takayama, J.M.Kanthi Abesundara, and T.Matsui, Assay of a-glucosidase inhibitory activity using flow-biosensor system, Anal. Chim. Acta, 10.1016/S0003-2670(02)01539-8, 479, 2, 135-141, 479, 135-141, 2003.02.
115. T.Matsui, H.Guth, and W.Grosch, A comparative study of potent odorants in peanut, hazelnut, and pumpkin seed oils on the basis of aroma extract dilution analysis (AEDA) and gas chromatography-olfactometry of headspace samples(GCOH), Fett/Lipid, 100, 2, 51-56, 100(2), 51-56, 1998.02.
116. Antihypertensive effect of sardine peptides in mild hypertensive subjects..
117. T.Matsui, T. Oki, and Y.Osajima, Isolation and identification of peptidic a-glucosidase inhibitors derived from sardine mucle hydrolyzate., Z. Naturforsch., 54, 3-4, 259-263, 54C, 259-263, 1999.02.
118. T.Oki, T.Matsui, and Y.Osajima, Inhibitory effect of a-glucosidase inhibitors varies according to its origin., J. Agric. Food Chem., 10.1021/jf980788t, 47, 2, 550-553, 47(2), 550-553, 1999.02.
119. T.Matsui, C. -H. Li, and Y.Osajima, Preparation and characterization of novel bioactive peptides responsible for angiotensin I-converting enzyme inhibition from wheat germ., J. Peptide Sci., 10.1002/(SICI)1099-1387(199907)5:73.0.CO;2-6, 5, 7, 289-297, 5, 289-297, 1999.05.
120. T.Matsui, K.Tamaya, T.Kawasaki, and Y.Osajima, Determination of angiotensin metabolites in human plasma by fluorimetric high-performance liquid chromatography using a heart-cut column-switching technique., J. Chromatogr.B, 10.1016/S0378-4347(99)00128-0, 729, 1-2, 89-95, 729, 89-95, 1999.07.
121. P.Pfnuer, T.Matsui, W.Grosch, H.Guth, and P.Schieberle, Development of a stable isotope dilution assay for the quantification of 5-methyl-(E)-2-heptene-4-one: Application to hazelnut oils and hazelnuts., J. Agric. Food Chem., 10.1021/jf9810214, 47, 5, 2044-2047, 47(5), 2044-2047, 1999.05.
122. Antihypertensive effect of sardine peptides and Val-Tyr - antihypertensive effect of the peptides in spontaneously hypertensive rats-.
123. Deviation of desorption equation of flavors from plastic films by using compatibility theory..
124. T.Oki, T.Matsui, and K. Matsumoto, Evaluation of a-glucosidase inhibition by using an immobilized assay system., Biol. Pharm. Bull., 23(9), 1084-1087, 2000.09.
125. Y-H.Hwang, T.Matsui, T.Hanada, M.Shimoda, K.Matsumoto, and Y.Osajima, Desorption behavior of sorbed flavor compounds from packaging films with ethanol solution., J. Agric. Food Chem., 10.1021/jf991082h, 48, 9, 4310-4313, 48(9), 427-431, 2000.09.
126. T. Kawasaki, E. Seki, K. Osajima, M. Yoshida, K. Asada, T. Matsui, and Y. Osajima, Antihypertensive effect of Valyl-Tyrosine, a short chain peptide derived from sardine muscle hydrolyzate, on mild hypertensive subjects., J. Human Hypertens., 10.1038/sj.jhh.1001065, 14, 8, 519-523, 14, 519-523, 2000.08.
127. Antihypertensive effect of sardine peptides in mild hypertensives and high-normal hypertensives..
128. T.Matsui, T.Ueda, T.Oki, K.Sugita, N.Terahara, and K.Matsumoto, a-Glucosidase inhibitory action of natural acylated anthocyanins. 1. Survey of natural pigments with potent inhibitory activity., J. Agric. Food Chem., 10.1021/jf001251u, 49, 4, 1948-1951, 49(4), 1948-1951, 2001.04.
129. T.Matsui, T.Ueda, T.Oki, K.Sugita, N.Terahara, and K.Matsumoto, a-Glucosidase inhibitory action of natural acylated anthocyanins. 2. a-Glucosidase inhibition by isolated acylated anthocyanins., J. Agric. Food Chem., 10.1021/jf0012502, 49, 4, 1952-1956, 49(4), 1952-1956, 2001.04.
130. T.Matsui, A.Yukiyoshi, S.Doi, H.Sugimoto, H.Yamada, and K.Matsumoto, Gastrointestinal enzyme production of bioactive peptides from royal jelly protein and their antihypertensive ability in SHR., J. Nutr. Biochem., 10.1016/S0955-2863(01)00198-X, 13, 2, 80-86, 13(2), 80-86, 2002.04.
131. T.Matsui, K.Tamaya, E.Seki, K.Osajima, K.Matsumoto, and T.Kawasaki, Val-Tyr as a natural antihypertensive dipeptide can be absorbed into human circulatory blood system., Clin. Exp. Pharmacol. Physiol., 10.1046/j.1440-1681.2002.03628.x, 29, 3, 204-208, 29(3), 204-208, 2002.03.
132. Chun-Hui Li, T. Matsui, K. Matsumoto, R. Yamasaki, and T. Kawasaki, Latent production of angiotensin I-converting enzyme inhibitors from buckwheat protein., J. Peptide Sci., 10.1002/psc.387, 8, 6, 267-274, 8, 267-274, 2002.05.
133. Depressor effect of sardine peptide-drink in mild hypertensives and normotensives..
134. Toshiro Matsui, Chun Hui Li, Toshiaki Tanaka, Tetsuyoshi Maki, Yutaka Osajima, Kiyoshi Matsumoto, Depressor effect of wheat germ hydrolysate and its novel angiotensin I- converting enzyme inhibitory peptide, Ile-Val-Tyr, and the metabolism in rat and human plasma, Biological and Pharmaceutical Bulletin, 23, 4, 427-431, 2000.04, We report here the antihypertensive effect of wheat germ (WG) hydrolysate and its dominant peptide, Ile-Val-Tyr (IVY), with potent angiotensin I-converting enzyme (ACE) inhibitory activity. The toxicity test of AG50W fraction purified from the WG hydrolysate and IVY in tidy mice revealed that 1 week median lethal concentrations of AG fraction and IVY were less than 100 and 10 mg/kg, respectively. As a result of an intravenous administration test of both inhibitors in spontaneously hypertensive rat (SHR), the mean arterial blood pressure (MAP) significantly decreased with the dose; the MAP reduction of 10.3 and 19.2 mmHg was observed at a dose of 50 mg/kg of AG fraction and 5 mg/kg of IVY, respectively. In addition to this behavior, the MAP gradually decreased after the 5 mg/kg of IVY injection (time to give a maximum reduction; 8 min), and the reduction was held for 20 min. By using rat and human plasma, IVY was found to be metabolized by the action of aminopeptidase in plasma to form a subsequent ACE inhibitor, Val- Tyr (VY). Thus, the intake of IVY as a physiologically functional food would serve in the lowering of blood pressure (BP) by the combined depressor effect of itself and its metabolite after the absorption..
135. Toshiro Matsui, Kei Tamaya, Kiyoshi Matsumoto, Yutaka Osajima, Keiko Uezono, Terukazu Kawasaki, Plasma concentrations of angiotensin metabolites in young male normotensive and mild hypertensive subjects, Hypertension Research, 22, 4, 273-277, 1999.04, The plasma concentrations of angiotensin (Ang) I, Ang II, and their metabolites (Ang (3-8), (4-8), (5-8), and (3-4)) following in vitro ACE inhibitory activity were examined in young male normotensive (NT) (n = 7), and mild hypertensive (HT) volunteers in = 6). There were no differences in supine plasma levels of Ang I, Ang II, and Ang (5-8) between the NT and HT groups: Ang I, 304 ± 43 fmol/ml vs. 293 ± 15 fmol/ml; Ang II, 32 ± 6 fmol/ml vs. 43 ± 10 fmol/ml; Ang (5-8), 176 ± 22 fmol/ml vs. 133 ± 32 fmol/ml. In addition, there were no significant differences between groups in any of these Ang levels when measured after standing for 60 min. However, the HT group showed significantly reduced supine and upright plasma Ang (3-8) and Ang (3-4) levels as compared to the NT group. In particular, the supine plasma level of Ang (3-4) (71 ± 13 fmol/ml-plasma) in the HT group was significantly (1/3-fold) lower than that in the NT group (197 ± 35 fmol/ml-plasma). An inverse correlation between the plasma level of Ang (3-4) and the upright systolic blood pressure (r = -0.627, p
136. Masaomi Fukamachi, Toshiro Matsui, Yoon Hee Hwang, Mitsuya Shimoda, Yutaka Osajima, Sorption Behavior of Flavor Compounds into Packaging Films from Ethanol Solution, Journal of Agricultural and Food Chemistry, 10.1021/jf950707i, 44, 9, 2810-2813, 1996.09, The sorption of flavor compounds into low-density polyethylene (LDPE) and ethylene-vinyl alcohol copolymer (EVOH) films was greatly influenced by ethanol concentration, and maximal sorptions were observed at 5-10% (v/v) ethanol for LDPE and 10-20% (v/v) ethanol for EVOH. The sorptions of ethyl hexanoate, octanal, and octanol into LDPE film were ∼1.7, 16.5, and 2.5 times higher than those in the 0% (v/v) ethanol solution, respectively (for EVOH film, the sorption increments of these compounds were ∼6.2, 3.5, and 4.1 times, respectively). To better understand this phenomenon, the solvent properties of ethanol solutions were investigated. The dielectric constants were roughly unchanged in the range 5-15% (v/v) ethanol. According to the results obtained by 1H NMR measurement, in which the difference in proton chemical shift from water-d (HDO) and ethyl alcohol-d (EtOD) became maximum at 23% (v/v) ethanol solution, the structural change of water induced by the addition of ethanol would be great up to 23% (v/v) ethanol. These results accurately reflected the sorption behavior. Therefore, the specific sorption behavior of flavor compounds from an ethanol solution would be brought about by the heterogeneous water-ethanol structure..
137. Hiroshi Matsufuji, Toshiro Matsui, Tomoyuki Oki, Yutaka Osajima, Terukazu Kawasaki, Fluorometric determination of angiotensins in human plasma derivatized with 2,3-naphthalenedialdehyde, Bunseki Kagaku, 10.2116/bunsekikagaku.44.783, 44, 10, 783-788, 1995.10, A fluorometric determination method for angiotensins (ANG I, II, III) in human plasma was developed with 2,3-naphthalenedialdehyde (NDA). Human plasma (10 ml) ultrafiltered with a Molucut L (
138. Hiroshi Matsufuji, Toshiro Matsui, Eiji Seki, Katsuhiro Osajima, Masatoshi Nakashima, Yutaka Osajima, Angiotensin i-converting enzyme inhibitory peptides in an alkaline protease hydrolyzate derived from sardine muscle, Bioscience, Biotechnology and Biochemistry, 10.1080/bbb.58.2244, 58, 12, 2244-2245, 1994.12, The ACE inhibitory activity of an alkaline protease hydrolyzate from sardine muscle did not change after being treated by gastrointestinal proteases (IC50 = 0.082mg protein/ml). Eleven new ACE inhibitory peptides, constructed with 2 to 4 amino acid residues, were isolated from the hydrolyzate. The ACE inhibitory activity of each was mostly below 100 μM of IC50 value; the maximal inhibitory activity was observed for Lys-Trp (IC50 = 1.63 μM). The isolated peptides inhibited ACE competitively, except for Met-Tyr with non-competitive inhibition. As the result of sequence homology, Arg-Val-Tyr isolated from the hydrolyzate was found in the primary structure of angiotensins I, II, and III, and of des Asp[1]-angiotensin I..
139. Toshiro Matsui, Hiroshi Matsufuji, Masatoshi Nakashima, Yutaka Osajima, Eiji Seki, Katsuhiro Osajima, Inhibition of Angiotensin I-converting Enzyme by Bacillus licheniformis Alkaline Protease Hydrolyzates Derived from Sardine Muscle, Bioscience, Biotechnology and Biochemistry, 10.1271/bbb.57.922, 57, 6, 922-925, 1993.06, Hydrolyzates which inhibit the angiotensin I-converting enzyme (ACE) were prepared from sardine muscle by Bacillus licheniformis alkaline protease. Considering the practical application of preparations as a functional food material, the best proteolytic conditions with respect to taste, solubility and ACE inhibitory activity were a 0.3 wt% addition of the enzyme and 17-h proteolysis at 50°C and pH 9.0. The preparations under these conditions had potent activity (IC50=0.26 mg protein/ml). Fractionation of the preparations on an ODS column with ethanol resulted in the production of more potent inhibitors. The most potent activity was obtained when eluting with 10% ethanol (IC50=0.015 mg protein/ml). This fraction was apparently rich in acidic amino acids, poor in hydrophobic ones, and effective for use as a physiologically functional food material by virtue of little bitterness, a fish odor and powerful ACE inhibitory activity..
1. Toshiro Matsui, Workshop on bioavailability and functions of food compounds, TUSZT (111 Base Project) 2022 Workshop, 2023.12.
2. Tint Ni Ni Tun、安部知純、相馬彩乃、西谷洋輔、栢木宏之、川上秀昭、松井利郎, Pharmacokinetic profiles of 3-(4-hydroxy-3-methoxyphenyl)propionic acidand its conjugates in Sprague-Dawley rats, 2023年度日本食品科学工学会西日本支部大会, 2023.10.
3. Yizhi Xiao, Risa Katagihara, Toshiro Matsui, Mitsuru Tanaka, Development of a Coumarin-Tagged MALDI-MS Technique and its Application for the Screening of Glycinin-Derived Peptides in Rat, 2023年度日本食品科学工学会西日本支部大会, 2023.10.
4. Xixi Li, Noa Nakashima, Arata Banno, Toshiro Matsui, Transport behavior of Hyp-containing dipeptides across Caco-2 cell monolayers, 2023年度日本食品科学工学会西日本支部大会, 2023.10.
5. Caiyue Shi, Lihong Cheng, Saya Nakamura, Nana Esaki, Yuka Ichiba, Mitsuru Tanaka, Kenta Sakai, Toshiro Matsui, Stimulation of adiponectin-receptor agonistic soy peptide, Tyr-Pro, on the acetylcholine nervous system in NE-4C cells, 2023年度日本食品科学工学会西日本支部大会, 2023.10.
6. Toshiro Matsui, Mouth-to-brain transport of Tyr-Pro with anti-cognitive effect in mouse, 3rd Int. Symposium on Bioactive Peptides, 2023.09.
7. Toshiro Matsui, Anti-cognitive peptides: a transport to brain, Workshop 2023 at Guelph University, 2023.09.
8. 劉 卓非、有馬継士郎、桑原 涼、西木直巳、田中 充、松井利郎, Graphitized sheet-assisted laser desorption ionization-mass spectrometry for simple and rapid detection of small organic compounds, 日本分析化学会 第72年会, 2023.09.
9. Lihong Cheng, Atsuko Yoshino, Yuki Nagasato, Fuyuko Takata, Shinya Dough, Mitsuru Tanaka, Toshiro Matsui, Oral intake of anti-cognitive impairment dipeptide, Tyr-Pro, leads to its intact accumulation in mouse brain, 第77回日本栄養・食糧学会大会, 2023.05.
10. Toshiro Matsui, Visualization analysis of intestinal absorption of polyphenols, 6th KBMSS Int. Symposium (第6回 京都生体質量分析研究会国際シンポジウム・国際質量分析イメージングシンポジウム2023京都), 2023.01.
11. Saya Nakamura, Guanzhen Gao, Sumire Asaba, Kenta Sakai, Yuji Miyata, Hisayuki Nakayama, Toshiro Matsui, Hesperidin promotes NO production and Mas receptor expression in human umbilical vein endothelial cells by binding to a transient receptor vanilloid 1, 22nd ICN (Int. Congress of Nutrition) in Tokyo, 2022.12.
12. Tae Hun Hahm, Toshiro Matsui, Mitsuru Tanaka, Quantitative MALDI-MS imaging analysis of tissue accumulated food compounds by fluorescence-assisted spraying method, 22nd ICN (Int. Congress of Nutrition) in Tokyo, 2022.12.
13. Sumire Asaba, Chou Chihsuan, Lei Fu, Toshiro Matsui, Anti-hyperglycemic effect of theaflavins in spontaneously diabetic Torii rats, 22nd ICN (Int. Congress of Nutrition) in Tokyo, 2022.12.
14. Haruna Ide, Keishiro Arima, Toshiro Matsui, Mitsuru Tanaka, Simultaneous detection of food compounds by a graphite carbon black-aided laser desorption ionization-mass spectrometry, 22nd ICN (Int. Congress of Nutrition) in Tokyo, 2022.12.
15. Tint Ni Ni Tun, Guanzhen Gao, Ayano Soma, Yosuke Nishitani, Hiroyuki Kayaki, Hideaki Kawakami, Toshiro Matsui, Intact and glucuronidated flavonoid metabolites can promote NO production in HUVECs, 22nd ICN (Int. Congress of Nutrition) in Tokyo, 2022.12.
16. Chizumi Abe, Ye Zhang, Kazuhiro Takao, Koji Ochiai, Toshiro Matsui, Bioavailability of prenylated isoflavones, glyceollins, in Sprague-Dawley rats, 22nd ICN (Int. Congress of Nutrition) in Tokyo, 2022.12.
17. Ayano Soma, Chizumi Abe, Tint Ni Ni Tun, Yosuke Nishitani, Hiroyuki Kayaki, Hideaki Kawakami, Toshiro Matsui, Absorption and tissue distribution of 3-(4-hydroxy-3-methoxyphenyl) propionic acid, a flavonoid metabolite, in rats, 22nd ICN (Int. Congress of Nutrition) in Tokyo, 2022.12.
18. Lihong Cheng, Atsuko Yoshino, Yuki Nagasato, Fuyuko Takata, Shinya Dohgu, Mitsuru Tanaka, Toshiro Matsui, Evidence on a brain transport of anti-cognitive dipeptide, Tyr-Pro, after oral administration to mice, 22nd ICN (Int. Congress of Nutrition) in Tokyo, 2022.12.
19. Risa Katagihara, Xiaojing Sheng, Satoshi Nagaoka, Mitsuru Tanaka, Toshiro Matsui, Metabolic behavior of soybean protein glycinin in rat bloodstream by a coumarin- tagged MALDI-MS analysis, 22nd ICN (Int. Congress of Nutrition) in Tokyo, 2022.12.
20. Kiyomi Ono, Weilin Shen, Reiko Nakao, Takeshi Nikawa, Toshiro Matsui, Intestinal absorption of heptapeptide QDGYMPW with anti-muscle atrophy effect in cells and rats, 22nd ICN (Int. Congress of Nutrition) in Tokyo, 2022.12.
21. Toshiro Matsui, Brain Barrrier Transportable Peptides and Functions in Mouse Brain, 22nd ICN (Int. Congress of Nutrition) in Tokyo, 2022.12.
22. 李 ユナ、中野明裕、中村紗彩、松井利郎, 骨格筋L6細胞における糖取り込みを促進する大豆由来トリペプチドの探索, 第76回日本栄養・食糧学会大会, 2022.06.
23. Toshiro Matsui, A trip of peptides to the blood and brain systems, TUSZT (111 Base Project) 2021 Symposium, 2021.10.
24. Toshiro Matsui, Intestinal and brain-transportable peptides, ISNFF2021 (13th International Conference and Exhibition for Nutraceuticals and Functional Foods), 2021.10.
25. Toshiro Matsui, Movements in Food Science and Technology: Through Japan-China exchange symposium, 第68回日本食品科学工学会大会(国際交流シンポジウム) Innovation and International Collaboration of Food Science and Technology ─ International Cooperation Towards Sustainable Future ─, 2021.08.
26. 中野明裕、田中充、實松敬介、松井利郎, 生体膜模倣タンパク質モデルを用いたAdipoR1アゴニスト食品成分の探索, 第68回日本食品科学工学会大会, 2021.08.
27. 大嶧正隆、Nguyen Thi Doan、田中 充、松井利郎, LC-MSを用いた中鎖・長鎖脂肪酸の一斉定量法の確立, 第68回日本食品科学工学会大会, 2021.08.
28. Tae Hun Hahm, Mitsuru Tanaka, Toshiro Matsui, Approach of MALDI-MS assay for quantitative analysis using a fluorescence probe, 第68回日本食品科学工学会大会, 2021.08.
29. Xiaojing Sheng、Satoshi Nagaoka、Marika Hashimoto、Masato Beppu、Kenichiro Tsukamoto、Emiko Yanase、田中 充、松井利郎, The intake of β-conglycinin can produce diverse oligopeptides in Wistar rats, 第75回日本栄養・食糧学会大会, 2021.07.
30. Lei Fu、Baorui Li、松井利郎, Theaflavins exert anti-diabetic effect via promoting incretin secretion in spontaneous diabetic Torii rats, 第75回日本栄養・食糧学会大会, 2021.07.
31. 李 ユナ、中村紗彩、松井利郎, A finding of adiponectin-like dipeptides in rat L6 myotubes, 第75回日本栄養・食糧学会大会, 2021.07.
32. 松井 利郎, An overview of peptide and protein absorption, 112th AOCS Annual Meeting & Expo, 2021.05.
33. Lei FU、安部知純、A.M. Nectoux、B. Li、松井利郎, Theaflavins inhibit glucose transport across Caco-2 cells through the downregulation of the Ca2+/AMP-activated protein kinase-mediated glucose transporter SGLT1, 令和2年度(2020年度)日本栄養・食糧学会九州・沖縄支部大会, 2020.11.
34. Ye Zhang, Kazuhiro Takao, Koji Ochiai, Kuni Sasaki, Toshiro Matsui, Prenylation enhances the absorbability of dietary isoflavones, Frontiersin Chemistry, Biology, and Earth Scienece-International Symposium between National Taiwan University and Kyushu University, 2019.12.
35. Shuwei Huang, A.M.Nectoux, Chizumi Abe, Naoto Ohno, Yuji Miyata, Kazunari Tanaka, Takashi Tanaka, Haruo Yamamoto, Toshiro Matsui , Characterization of in vivo metabolism of hesperidin in Spraue-Dawley rat circulation, Frontiersin Chemistry, Biology, and Earth Scienece-International Symposium between National Taiwan University and Kyushu University, 2019.12.
36. Chizumi Abe, A.M.Nectoux, Shuwei Huang, Naoto Ohno, Junji Tabata, Yuji Miyata, Kazunari Tanaka, Takashi Tanaka, Haruo Yamamoto, Toshiro Matsui , A condensed catechin, theasinensin A, can promote the transport of hesperidin in Caco-2 cell monolayers: dynamics and mechanism, Frontiersin Chemistry, Biology, and Earth Scienece-International Symposium between National Taiwan University and Kyushu University, 2019.12.
37. Yuna Lee, Ruchia Kojima, Baorui Li, Ayaka Yamamoto, Tomoya Ueno, Toshiro Matsui, Anti-prediabetic effect of theaflavin in Spontaneously diabetic Torii rats, Frontiersin Chemistry, Biology, and Earth Scienece-International Symposium between National Taiwan University and Kyushu University, 2019.12.
38. Juncha Bak, Yoshiyuki Miyazaki, Toshiro Matsu, Ligand-aided quantitative and selective NMR assay for sulfated saccharides in solution, Frontiersin Chemistry, Biology, and Earth Scienece-International Symposium between National Taiwan University and Kyushu University, 2019.12.
39. Hayato Kiyohara, Mitsuru Tanaka, Shinya Dohgu, Atsuko Yoshino, Genki Kobayashi, Fuyuko Takata, Yasufumi Kataoka, Takashi Nirasawa, Toshiro Matsui, Brain transportable dipeptides across the blood-brain barrier in mice, Frontiersin Chemistry, Biology, and Earth Scienece-International Symposium between National Taiwan University and Kyushu University, 2019.12.
40. Xiaojing Sheng, Satoshi Nagaoka, Marika Hashimoto, Yusuke Amiya, Emiko Yanase, Mitsuru Tanaka, Toshiro Matsui, Identification of metabolic peptides from dietary syprotein, b-conglycinin, in rat blood, Frontiersin Chemistry, Biology, and Earth Scienece-International Symposium between National Taiwan University and Kyushu University, 2019.12.
41. Wellin Shen, Mitsuru Tanaka, Toshiro Matsui, Elucidation of the Intestinal transport behavior of model oligopeptide designed based on Gly-Sar, Frontiersin Chemistry, Biology, and Earth Scienece-International Symposium between National Taiwan University and Kyushu University, 2019.12.
42. Tae Hun Ham, Huu Nghi Nguyen, Mitsuru Tanaka, Ayaka Tsutsumi, Qing Qiang Hu, Koichi Aizawa, Toshiro Matsui, Visualization of rat intestinal absorption behavior of anthocyanins by matrix assisted laser desorption/ionization imaging mass spectrometry, Frontiersin Chemistry, Biology, and Earth Scienece-International Symposium between National Taiwan University and Kyushu University, 2019.12.
43. Mitsuru Tanaka, Toshiro Matsui, Importance of the ADME analysis or peptide functionality, Frontiersin Chemistry, Biology, and Earth Scienece-International Symposium between National Taiwan University and Kyushu University, 2019.12.
44. Tae Hun Ham, Huu Nghi Nguyen, Mitsuru Tanaka, Ayaka Tsutsumi, Qing Qiang Hu, Koichi Aizawa, Toshiro Matsui, Intestinal absorption of visualized anthocyanins in rats by matrix assisted laser desorption/ionization mass spectrometry imaging techinique, ICoFF/ISNFF2019 (12th International Conference and Exhibition for Nutraceuticals and Functional Foods), 2019.12.
45. A.M.Nectoux, Chizumi Abe, Shuwei Huang, Naoto Ohno, Junji Tabata, Haruo Yamamoto, Toshiro Matsui, Absorption and metabolic behavior of hesperidin (rutinosylated hesperetin) after a single oral administration to Sprague-Dawley rats, ICoFF/ISNFF2019 (12th International Conference and Exhibition for Nutraceuticals and Functional Foods), 2019.12.
46. Ye Zhang, Kazuhiro Takao, Kuni Sasaki, Koji Ochiai, Toshiro Matsui, Enhanced absorption of prenylated isoflavones in rat circulating bloodstream, ICoFF/ISNFF2019 (12th International Conference and Exhibition for Nutraceuticals and Functional Foods), 2019.12.
47. Wellin Shen, Mitsuru Tanaka, Reiko Nakao, Takeshi Nikawa, Toshiro Matsui, Absorption of Cblin oligopeptide across Caco-2 cell monolayers and rat intestinal membrane, ICoFF/ISNFF2019 (12th International Conference and Exhibition for Nutraceuticals and Functional Foods), 2019.12.
48. Hayato Kiyohara, Mitsuru Tanaka, Shinya Dohgu, Genki Kobayashi, Fuyuko Takata, Yasufumi Kataoka, Toshiro Matsui, Dipeptides can transport across the blood-brain barrierin mouse brain, ICoFF/ISNFF2019 (12th International Conference and Exhibition for Nutraceuticals and Functional Foods), 2019.12.
49. Juncha Bak, Yoshiyuki Miyazaki, Toshiro Matsui, An establishement of NMR-based analytical assay for sulfated saccharides in solution, ICoFF/ISNFF2019 (12th International Conference and Exhibition for Nutraceuticals and Functional Foods), 2019.12.
50. Chizumi Abe, Naoto Ohno, Shuwei Huang, A.M.Nectoux, Haruo Yamamoto, Toshiro Matsui, Theasinensin A promotes paracellular transport of hesperidin in Caco-2 cell, ICoFF/ISNFF2019 (12th International Conference and Exhibition for Nutraceuticals and Functional Foods), 2019.12.
51. Mitsuru Tanaka, Tae Hun Ham, Hun-Nghi Nguyen, Toshiro Matsui, Visualization of absorption and metabolism of polyphenols in rat intestine by nifedipine-aided MALDI-MS imaging, ICoFF/ISNFF2019 (12th International Conference and Exhibition for Nutraceuticals and Functional Foods) , 2019.12.
52. Toshiro Matsui, Application of MALDI-MS imaging technique for intestinal absorption process of bioactive peptides, ICoFF/ISNFF2019 (12th International Conference and Exhibition for Nutraceuticals and Functional Foods) , 2019.12.
53. Toshiro Matsui, Theaflavins, a regulator of intestinal absorption: action and mechanism, 2019 International Symposium of Food and Health, 2019.11.
54. Toshiro Matsui, Importance of the ADME analysis for the evaluation of food functionality-focusing on intestinal absorption -, 2019 International Symposium of Food and Nutrition, 2019.10.
55. 黄 書薇、大野直土、安部知純、A.M.Nectoux、宮田裕次、田中 隆、山村春男、松井利郎, Promoting transport behavior of hesperidin and its metabolism in theasinensin A-treated Caco-2 cells, 2019年度日本栄養・食料学会九州・沖縄支部および日本食品科学工学会西日本支部合同大会, 2019.10.
56. 李 宝瑞、小島瑠知亜、山本采佳、上野友哉、松井利郎, Theaflavin-3'gallate inhibits glucose transport in Caco-2 cells by down-regulating the expression of glucose transporter 2 via AMPK signal pathway activation, 第66回日本食品科学工学会, 2019.08.
57. Toshiro Matsui, Absorption behavior of bioactive peptides across intestinal membrane, 2nd International Symposium on Bioactive Peptides (ISBP2019) , 2019.05.
58. 松井 利郎, Transport of oligopeptides across Caco-2 cell monolayers, 11th ISNFF, 2018.10.
59. 黄 書薇,大野直土,安部知純,田端淳二,宮田裕次,田中一成,田中 隆,山村春男,松井利郎, Enhanced absorption of hesperidin by coexistent theasinensin A in Caco-2 cell monolayers and Sprague-Dawley rats, 2018年度日本食品科学工学会西日本支部大会, 2018.11.
60. Sato Ritsuko,小島瑠知亜,松井利郎, Identification of isoflavone-derived metabolites in soybean and their promoting effect on glucose uptake in L6 myotube, IFT2018, 2018.07.
61. 鐘 璇,田尻聡史,俵口真衣,小柳里穂,志村昭弘,松井利郎, Simultaneous separation of catecholamine metabolites on a sulfonated mixed-mode copolymer column by liquid chromatography mass spectrometry, 第72回日本栄養・食糧学会大会, 2018.05.
62. 申 偉琳,田中 充,松井利郎, Simultaneous detection of angiotensin metabolites in rat plasma using LC-MS/MS method, 第72回日本栄養・食糧学会大会, 2018.05.
63. Ritsuko Sato, Risa Yoshida, Naoki Morishita, Toshiro Matsui, Koji Ochiai, Baorui Li, Yusuke Terazono, Naoto Hirasaki, Emiko Kinoshita, Akio Obata, Toshiro Matsui, Masatoshi Shinagawa, Identification of isoflavone-derived metabolites in soybean and their promoting effect on glucose uptake in L6 myotubes, 平成29年度日本食品科学工学会西日本支部および日本栄養・食糧学会九州・沖縄支部合同大会, 2017.10.
64. 松井 利郎, Effect of aging on vasorelaxation of small peptides on contracted rat aorta, ISNFF2017, 2017.10.
65. 田中 充、松井 利郎, Screening of synergic combination of food compounds to exert vasorelaxation effect in rat aorta, ISNFF2017, 2017.10, SDラット大動脈血管を用いて、フェルラ酸等のフェノール酸による血管弛緩を促進させる他の食品成分を明らかにし、その相乗作用について報告した。.
66. 大野直土、田端淳二、松井 利郎, Intestinal absorption of hesperidin and its metabolites in Sprague-Dawley rats, ISNFF2017, 2017.10.
67. 網屋祐輔、田中 充、松井 利郎, Optimization of MALDI-MS detection of bioactive peptides in rat plasma, ISNFF2017, 2017.10.
68. Baorui Li, Yusuke Terazono, Naoto Hirasaki, Emiko Kinoshita, Akio Obata, Toshiro Matsui, Tomatoside A inhibits glucose transport across Caco-2 cell monolayers, 第64回日本食品科学工学会大会, 2017.08.
69. Hsusan Chung, Akihiro Shimura, Toshiro Matsui, Simultaneous and discriminant HPLC analysis of reducing and non-reducing monosaccharides on a polyethylene-attached hydrophilic interaction liquid chromatography column, 日本食品分析学会平成29年度学術集会, 2017.08.
70. 松井 利郎, Absorption of peptides into rat blood: Effect of peptide-length and aging of rats on absorption, 108th AOCS Annual Meeting & Expo, 2017.05.
71. 松井 利郎, Small peptides: Anti-hypertensive and vasorelaxation actions, International Symposium on Bioactive Peptides, 2017.05, 低分子ペプチドの生理作用と吸収挙動について招待講演を行った。.
72. A. Siltari, W. Shen, R. Yoshida, T. Matsui, R. Korpela, H. Vapaatalo, Absorption of bioactive tripeptides across Caco-2 cell membrane and rat intestine in vitro., 5th International Conference on Food Digestion, 2017.04.
73. 松井 利郎, Vasorelaxation mechanism of small peptides in contracted aged aorta, 9th ISNFF, 2016.10.
74. Nguyen Huu Nghi, 田中 充, 松井 利郎, Enhanced matrix-assisted MALDI-MS detection of polyphenol by nifedipine a photobase generator, 日本分析化学会第65回年会, 2016.09.
75. 鐘 璇, Nguyen Thu Huong, 志村昭弘, 松井 利郎, Discriminant analysis of non-reducing and reducing rare sugars on a prototype hydrophilic interaction liquid chromatography (HILIC) column, 第2回日本食品分析学会, 2016.09.
76. Vu-Thi Hanh, 小林優多郎, 田中 充, 松井 利郎, Application of a standard addition method for quantitative mass spectrometry assay of dipeptides, 第63回日本食品科学工学会大会, 2016.08.
77. 郭 建, 平崎直人, 田中 隆, 松井 利郎, Interaction of theasinensin A with hydrophobic dipeptides in water by NMR analysis, 第70回日本栄養・食糧学会大会, 2016.05.
78. 松井 利郎, Design of oligopeptides for intestinal absorption model, 107th AOCS Annual Meeting & Expo, 2016.05.
79. 松井 利郎, Intestinal absorption of bioactive compounds, The 4th Nutrition Winter School, 2016.01.
80. 松井 利郎, Health-benefit of small peptides, Symposium of bioactive compounds, 2016.01.
81. Toshihiko Fukuda, 松井 利郎, Yoshinori Mine, Adenine, a vasorelaxant purine, reduces intestinal inflammation in colitis mouse model, The international Chemical Congress of Pacific Basin Societies, 2015.12.
82. 松井 利郎, Mechanism of vasorelaxation of small peptides via the suppression of intracellular Ca2+ signaling pathways, 8th Meeting of the International Society of Nutrition and Functional Foods (ISNFF), 2015.09.
83. Si Jing Chen, Chiwa Aikawa, 松井 利郎, Advanced glycation end-products in spontaneously diabetic Torii rats at pre-diabetic stage by liquid-chromatography-tandem mass spectrometry, 日本分析化学会第64年会, 2015.09.
84. Jiang Guo, Akihiro Shimura, 松井 利郎, Elution profile of di/tri-peptides on sulfonated ethylstylene-divinylbenzene copolymer resin column by high-performance liquid chromatography, 日本分析化学会第64年会, 2015.09.
85. 陳 思婧, 相川知和, 吉田理紗, 松井 利郎, High-sensitive detection of advanced glycation end-products and its application for the prevention of of pre-diabetic diseases, 第33回分析化学会九州分析若手の会夏季セミナー, 2015.07.
86. Mai Hyoguchi, Toshihiko Fukuda, Miki Kono, Mitsuru Tanaka, 松井 利郎, Age-related vasorelaxation of natural bioactive compounds, The 2nd Taiwan-Japan Food Science, Technology and Industry Symposium, 2015.05.
87. Naoto Hirasaki, Yuri Kunitake, Ha-Yong Park, Mitsuru Tanaka, 松井 利郎, Theaflavins can enhance the intestinal barrier in Caco-2 cells, The 2nd Taiwan-Japan Food Science, Technology and Industry Symposium, 2015.05.
88. Mitsuru Tanaka, Jian Zhao, Aki Suyama, 松井 利郎, Ferulic acid improves acetylcholine-induced nitric oxide production via up-regulation of argnitrosaccinate synthase in TNF-alpha-stimulated inflammatory human umbilical vein endothelium cells, 12th Asian Congress of Nutrition, 2015.05.
89. Shizuka Tamaru, Mimi Iha, Sayaka Shimamoto, Hisayuki Nakayama, Sadayuki Aramaki, Yuji Miyata, Takashi Tanaka, 松井 利郎, Yasuo Nagata, Kazunari Tanaka, Effect of mixed tea made with unripe citrus Unshu and third-crop green tea leaves on hepatic lipid accumulation in rats, 12th Asian Congress of Nutrition, 2015.05.
90. Kazuki Moriyasu, Takashi Ichinose, Akane Nakahara, Motohiro Maebuchi, Mitsuru Tanaka, 松井 利郎, 古屋 茂樹, The oral supplementation of soy protein-derived dipeptides accelerates the neurotransmitter metabolism, 12th Asian Congress of Nutrition, 2015.05.
91. Toshihiko Fukuda, Takanori Kuroda, miki Kono, Mai Hyoguchi, 松井 利郎, Inhibition of Ca2+/calmodulin signaling on adenine-induced vasorelaxation in vascular smooth muscle cells, 12th Asian Congress of Nutrition, 2015.05.
92. Si Jing Chen, Chiwa Aikawa, Mitsuru Tanaka, 松井 利郎, Plasma levels of advanced glycation end-products during pathogenic progress of diabetes in spontaneously diabetic Torii rats, 12th Asian Congress of Nutrition, 2015.05.
93. Gonzalo Miyagusuku, Masaya Kono, Kayo Yoshiyama, Keiichi Fukui, Norihiko Terahara, 松井 利郎, 6-O-Caffeolysophorose enhances glucose uptake in L6 cells by induced Glut4 translocation, 12th Asian Congress of Nutrition, 2015.05.
94. 松井 利郎, Intestinal absorption of bioactive peptides: detection and visualization, 106th AOCS Meeting, 2015.05.
95. 福田俊彦, 黒田高広, 河野美紀, 松井 利郎, Adenine, a vasorelaxant purine, regulates Ca2+ signialing through PLC/CaMKII/MLC pathway in vascular smooth muscle cells, 7th International Conference and Exhibition on Nutraceutical and Functional Foods (ISNFF), 2014.10.
96. Thanutchaporn Kumrungsee, 秋山紗綾香, 松井 利郎, In vivo and in silico analysis for calcium-calmodulin complex formation, 日本分析化学会第63年会, 2014.09.
97. Jian Guo, Thanutchaporn Kumrungsee, 佐伯知美, Wanying Liu, Akihiro Shimura, 松井 利郎, Application of cation-exchemgel reversed-phase mixed-mode HPLC column for the separation of small peptides, 日本分析化学会第63年会, 2014.09.
98. 朴 夏英, 國武友里, 松井 利郎, Enhancement of intestinal barrier by theaflavins through the expression of AMP-activated protein kinase mediated tight junction related proteins, The 36th ESPEN (The European society for clinical nutrition and metabolism), 2014.09.
99. 朴 夏英, 國武友里, 松井 利郎, Enhancement of intestinal barrier by theaflavins through increasing the expression of AMP-activated protein kinase mediated tight junction-related proteins occuludin, claudin-1 and ZO-1, The XXVⅡth International Conference on Polyphenols, 2014.09.
100. Gonzalo Miyagusuku, 幸野将也, 吉山かよ, 福井敬一, 寺原典彦, 松井 利郎, Enhanced glucose uptake in L6 cells by 6-O-Caffeoylsophorose, 第61回日本食品科学工学会大会, 2014.08.
101. Yoshinori Mine, Jennifer Kovacs-Nolan, 松井 利郎, 田中 充, Toru Konishi, Yusuke Sauchi, Transport of intact glutathione across human intestinal epithelial cell monolayers using Caco-2 and HT-29 models, 248th ACS National Meeting, 2014.08.
102. 曹 汝鴿, 野中愛理, 松井 利郎, The application of diffusion ordered-‘H-nuclear magnetic resonance spectroscopy to evaluate the food quality, 第32回分析化学会九州分析若手の会夏季セミナー, 2014.07.
103. 小林優多郎, Yoshinori Mine, 松井 利郎, Anti-inflammatory effect of the vasoprotective di-peptides in intestinal mucosa system, 105th AOCS Annual Meeting & Expo, 2014.05.
104. Matsui Toshiro, Vasoactivity of small peptides and high-sensitive detection of absorbed peptides, 2013 Int. Symposium of Functional Foods Research Center, 2013.11.
105. Matsui Toshiro, Physiological potentials of condensed polyphenols in intestine and muscle tissues, 2013 Int. Conference of the Korean Society of Food Science and Nutrition, 2013.11.
106. 朴 夏英, 國武友里, Matsui Toshiro, Theaflavins enhance the intestinal barrier through AMP-activated protein kinase-mediated expression of tight-junction-related proteins, 2013 Int. Conference of the Korean Society of Food Science and Nutrition, 2013.11.
107. 國武友里, 朴 夏英, Matsui Toshiro, Theaflavins inhibit peptide transport across Caco-2 cell monolayers via down-regulation of peptide transporter PEPT1, 2013 Int. Conference of the Korean Society of Food Science and Nutrition, 2013.11.
108. Matsui Toshiro, High-sensitive detection and visualization of vasoactive small peptides, 2013 Symposium on Analysis and Efficacy of Functional Foods in East Asia Region, 2013.10.
109. Gozalo Miyagusuku, 幸野将也, 橋本千尋, 福井敬一, 田中 充, 松井 利郎, 6-O-Caffeoylsophorose stimulates glucose uptake in L6 myotubes through IRS-PI3K activation, 平成25年度日本栄養・食糧学会九州・沖縄支部及び日本食品科学工学会西日本支部合同大会, 2013.10.
110. 趙 健, 須山 晶, 田中 充, 松井 利郎, Ferulic acid enhances vasorelaxation effect of epigallocatechin gallate in tumor necrosis factor-a-induced inflammatory rat aorta, 第60回日本食品科学工学会, 2013.08.
111. 陳 思婧, 橋本千尋, 相川知和, 松井 利郎, Improvement of advanced glycation end products detection by liquid-chromatography-tandem mass spectrometry with 2,4,6-trinitrobenzen sulfonate derivatization, Asianalysis XII, 2013.08.
112. 曹 汝鴿, 野中愛理, 小村英恵, 松井 利郎, Application of diffusion ordered-nuclear magnetic resonance spectroscopy to quantify glucose and sucrose in some beverages, Asianalysis XII, 2013.08.
113. T.Kumrungsee, 佐伯知美, 松井 利郎, Ca-CaM complex formation inhibitory activity of bioactive peptide, Trp-His, by in vitro and in silica analyses, Asianalysis XII, 2013.08.
114. 朴 夏英, 竹田隼子, 國武友里, 松井 利郎, Theaflavins enhance the intestinal barrier by increasing the expression of tight junction proteins via activation of AMP-activated protein kinase, 13th International Conference of FFC, 2013.05.
115. 國武友里, 朴 夏英, 松井 利郎, Theaflavins can close tight-junction via activation of AMP-activated protein kinase in Caco-2 cell monolayers, 2013 International Students Academic Conference, 2013.03.
116. 洪 性珉, 吉井沙織, 盛田裕太郞, Mitsuru Tanaka, 松井 利郎, Application of MALDI-MS imaging to visualize the tissue distribution of absorbable small peptides in rat intestine, 2013 International Students Academic Conference, 2013.03.
117. 福田俊彦, 徳田信二, 小林優多郎, Mitsuru Tanaka, 松井 利郎, Endothelium-independent vasorelaxation of adenine in contracted thoracic aorta of Sprague-Dawley rats, 2012 International Students Academic Conference, 2012.03.
118. 朴 夏英, 竹田 隼子, 松井 利郎, Theaflavins regulate the absorption activity and expression of the peptide transporter PEPT-1 in Caco-2 cells, 2012 International Students Academic Conference, 2012.03.
119. 松井 利郎, Functional Food Compounds : Vasorelaxation and Absorption, 2012 International Students Academic Conference, 2012.03.
120. 王 正全、田中 充、渡邊慎平、小林優多郎、松井利郎, Anti-atherosclerotic di-peptide, Trp-His, can suppress intracellualr [Ca2+]i in vascular smooth muscle cells in a similar manner to dihydropyridine type Ca2+ channel blocker, The 23rd Scientific Meeting of the International Society of Hypertension, 2010.09.
121. Bioactive peptides and hypertension.
  • 学外
  • 学外
Educational Activities
Undergraduate School:
Analytical Chemistry, Engineering for Food Analysis, Scientific English I, and II, Food Science for G30, Applied Biological Science, Experiment for Fundamental Analytical Chemistry, Experiment for Food Analysis
Graduate School:
Seminar on Engineering Analysis, Food Science and technology I and II, Functional Food、Food Science and Food System, Food Science and Biotechnology
Other Educational Activities
  • 2023.12.
  • 2023.08.
  • 2022.12.
  • 2022.08.
  • 2021.12.
  • 2021.03.
  • 2020.12.
  • 2020.08.
  • 2020.03.
  • 2019.08.
  • 2019.03.
  • 2018.08.
  • 2017.08.
  • 2017.10.
  • 2016.08.
  • 2016.10.
  • 2015.08.
  • 2015.10.
  • 2013.10.
  • 2013.09.
  • 2013.08.
  • 2013.02.
  • 2010.08.
  • 2009.08.
  • 2008.11.
  • 2006.11.
  • 2004.08.
  • 2003.08.
  • 2003.03.
  • 2002.07.
Professional and Outreach Activities
2002- Guest researcher of Nagasaki Pref. Industry Center
2001-2002 Guest researcher of Hiroshima Pref. Food Industry Center
2016- Lecture at University of Helsinki
2020- Tianjin University of Science & Technology (TUST 111 Base Project) Foreign Expert Professor.