Kyushu University Academic Staff Educational and Research Activities Database
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Ken-ichi Honjoh Last modified date:2022.01.08

Associate Professor / Division of Food Science and Biotechnology
Department of Bioscience and Biotechnology
Faculty of Agriculture

Graduate School
Undergraduate School

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Academic Degree
Ph.D of Agriculture
Country of degree conferring institution (Overseas)
Field of Specialization
Food Preservation
Total Priod of education and research career in the foreign country
Outline Activities
Some plant species develop freezing tolerance when exposed to low, non-freezing temperatures. If freezing tolerance could be provided to cold-sensitive plants, the contribution to stable production of crops would be significant. In our laboratory, we found that hardened cells of Chlorella vulgaris C-27 survive slow freezing to -196°C. We are investigating the mechanism of the development of freezing tolerance of Chlorella. Furthermore, based on the research of Chlorella, enhancement of freezing tolerance of higher plants is also carried out.
Research Interests
  • Development of a method for lowering contamination with Listeria to fresh produce during cultivation
    keyword : Listeria monocytogenes, fresh produce, decontamination
  • Studies on new compatible solutes for freezing tolerance
    keyword : compatible solutes, freezing tolerance, taurine, tolehalose
  • Studies on the mechanisms of the development of freezing tolerance of chlorella
    keyword : chlorella, freezing tolerance, hardening
    1991.04~2017.03Studies on the mechanism of the development of freezing tolerance of Chlorella.
  • Studies of enhancement of freezing tolerance of Saccharomyces cerevisiae
    keyword : freezing tolerance, Saccharomyces cerevisiae
    1996.04Enhancement of freezing tolerance of yeast.
  • Studies on the enhancement of freezing tolerance of higher plants
    keyword : freezing tolerance, Arabidopsis thaliana, lettuce, plants
    1994.04Enhancement of freezing tolerance of higher plants.
Academic Activities
1. Takeshi Machida, Akiko Ishibashi, Eri Kato, Jun-ichi Sato, Shinji Kawasaki, Youichi Niimura, Ken-ichi Honjoh and Takahisa Miyamoto, Chloroplast NADPH-dependent thioredoxin reductase from Chlorella vulgaris functions as an antioxidant system with 2-Cys peroxiredoxin; The 5th International Joint Symposium between Japan and Korea. The Recent Status and Perspective of Food System, Agricultural Environment and Biology, 2008.12.
1. Ken-ichi HONJOH, Hitomi OKANO, Aya KAWABATA, Masaru KUROKAWA, Taiki KIMURA, Takeshi MACHIDA, Yoshimitsu MASUDA and Takahisa MIYAMOTO, Freezing tolerance of Lactuca sativa and induction of CBF and GolS genes during cold treatment, Journal of Faculty of Agriculture, Kyushu University, 63, 2, 249-257, 2018.09.
2. Approaches of Improvement of Freezing-Stress Tolerance of Yeast.
3. Machida, T., Kato, E., Ishibashi, A., Ohashi, N., Honjoh, K., Miyamoto, T., Molecular characterization of low-temperaure-inducible NTR-C in Chlorella vulgaris, Nucleic Acids Symposium Series, No. 51, 463-464, 2007.11.
4. Suga, K., Honjoh, K., Furuya, N., Shimizu, H., Nishi, K., Shinohara, F., Hirabaru, Y., Maruyama, I., Miyamoto, T., Hatano, S., and Iio, M.,, Two low-temperature-inducible Chlorella genes for D12 and w-3 fatty acid desaturase (FAD): Isolation of D12 and w-3 fad cDNA clones, expression of D12 fad in Saccharomyces cerevisiae , and expression of w-3 fad in Nicotiana tabacum, Biosci. Biotechnol. Biochem., 66, 6, 1314-1327, 66(6): 1314-1327, 2002.06.
5. Honjoh, K., Matsumoto, H., Shimizu, H., Ooyama, K., Tanaka, K., Oda, Y., Takata, R., Joh, T., Suga, K., Miyamoto, T., Iio, M. and Hatano, S., Cryoprotective activities of group3 late embryogenesis abundant proteins from Chlorella vulgaris C-27, Biosci. Biotechnol. Biochem., 10.1271/bbb.64.1656, 64, 8, 1656-1663, 64(8): 1656-1663, 2000.08.
6. Joh, T., Yoshimoto, M., Honjoh, K., Miyamoto, T., Hatano, S., Changes in translatable RNA population during hardening of Chlorella ellipsoidea C-27., J. Fac. Agr., Kyushu Univ., 37, 3-4, 257-263, 37(3,4), 257-263, 1993.02.
1. Kimura, T., Kurokawa, M., Honjoh, K., Masuda, Y., Miyamoto T., Functional analysis of galactinol synthase gene from Lactica sativa in Escherichia coli, JSBBA West 1st Student Forum, 2018.11.
2. Ken-ichi Honjoh, Tomoko Mishima, Nozomi Kido, Yuri Iwaizako, Ying Lin, Takahisa Miyamoto, Studies on contamination routes of Salmonella Enteritidis and Listeria monocytogenes in vegetables, 43rd Annual Meeting United States - Japan Cooperative Program in Natural Resources Food and Agricultural Panel, 2014.10.
3. Ken-ichi Honjoh, Hitomi Okano, Aya Kawabata, Ayako Shiraishi, Takahisa Miyamoto, Molecular response of Lactuca sativa L. to low temperature, 13TH ASEAN FOOD CONFERENCE 2013, 2013.09.
Membership in Academic Society
  • Japanese Society for Safety Education
  • Japanese Society for Plant Cell and Molecular Biology
  • Japan Society for Bioscience, Biotechnology, and Agrochemistry
  • The Japanese Society for Food Science and Technology
  • The Japanese Society of Plant Physiologists
  • Japanese Society of Food Microbiology