


Ken-ichi Honjoh | Last modified date:2020.10.26 |

Associate Professor /
Division of Food Science and Biotechnology
Department of Bioscience and Biotechnology
Faculty of Agriculture
Department of Bioscience and Biotechnology
Faculty of Agriculture
Graduate School
Undergraduate School
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Homepage
https://kyushu-u.pure.elsevier.com/en/persons/ken-ichi-honjoh
Reseacher Profiling Tool Kyushu University Pure
http://www.agr.kyushu-u.ac.jp/lab/foodhygienicchemistry/eindex.html
Phone
092-802-4758
Fax
092-802-4757
Academic Degree
Ph.D of Agriculture
Country of degree conferring institution (Overseas)
No
Field of Specialization
Food Preservation
Outline Activities
Some plant species develop freezing tolerance when exposed to low, non-freezing temperatures. If freezing tolerance could be provided to cold-sensitive plants, the contribution to stable production of crops would be significant. In our laboratory, we found that hardened cells of Chlorella vulgaris C-27 survive slow freezing to -196°C. We are investigating the mechanism of the development of freezing tolerance of Chlorella. Furthermore, based on the research of Chlorella, enhancement of freezing tolerance of higher plants is also carried out.
Research
Research Interests
Membership in Academic Society
- Development of a method for lowering contamination with Listeria to fresh produce during cultivation
keyword : Listeria monocytogenes, fresh produce, decontamination
2013.05. - Studies on new compatible solutes for freezing tolerance
keyword : compatible solutes, freezing tolerance, taurine, tolehalose
2009.04. - Studies on the mechanisms of the development of freezing tolerance of chlorella
keyword : chlorella, freezing tolerance, hardening
1991.04~2017.03Studies on the mechanism of the development of freezing tolerance of Chlorella. - Studies of enhancement of freezing tolerance of Saccharomyces cerevisiae
keyword : freezing tolerance, Saccharomyces cerevisiae
1996.04Enhancement of freezing tolerance of yeast. - Studies on the enhancement of freezing tolerance of higher plants
keyword : freezing tolerance, Arabidopsis thaliana, lettuce, plants
1994.04Enhancement of freezing tolerance of higher plants.
Books
Papers
1. | Ken-ichi HONJOH, Yin LIN, Kiyomi JO, Yuri IWAIZAKO, Masayuki MAEDA, Nobuyuki KIJIMA, and Takahisa MIYAMOTO, Possible contamination routes of Listeria monocytogenes to leaf lettuce during cultivation, Food Science and Technology Research, 24, 5, 911-920, 2018.09. |
2. | Ken-ichi HONJOH, Hitomi OKANO, Aya KAWABATA, Masaru KUROKAWA, Taiki KIMURA, Takeshi MACHIDA, Yoshimitsu MASUDA and Takahisa MIYAMOTO, Freezing tolerance of Lactuca sativa and induction of CBF and GolS genes during cold treatment, Journal of Faculty of Agriculture, Kyushu University, 63, 2, 249-257, 2018.09. |
3. | Honjoh, K., Iwaizako, Y., Lin, Y., Kojima, N., Miyamoto, T., Possibilities of contamination of tomato fruits by Listeria monocytogenes during cultivation, Food Science and Technology Research, https://doi.org/10.3136/fstr.22.349, 22, 3, 349-357, 2016.05, [URL]. |
4. | Approaches of Improvement of Freezing-Stress Tolerance of Yeast. |
5. | Honjoh, K., Mishima, T., Kido, N., Shimamoto, M., Miyamoto, T., Investigation of possible contamination routes of Salmonella via soils and the effects of mulch for avoiding its contamination to leafy lettuce plants during cultivation, Food Science and Technology Research, https://doi.org/10.3136/fstr.20.961, 20, 5, 961-969, 2014.09, [URL]. |
6. | Machida, T., Ishibashi, A., Kirino, A., Sato, J., Kawasaki, S., Niimura, Y., Honjoh, K., Miyamoto, T., Chloroplast NADPH-dependent thioredoxin reductase from Chlorella vulgaris alleviates environmental stresses in yeast together with 2-Cys peroxiredoxin, PLoS ONE, 7, 9, e45988, 2012.09. |
7. | Machida, T., Honjoh, K., Aso, A., Yamamoto, M., Iio, M., Miyamoto, T., Trehalose 6-phosphate synthase and trehalose 6-phosphate phosphatase from Nicotiana tabacum function in trehalose biosynthesis and environmental stress tolerance of yeast, Journal of Faculty of Agriculture, Kyushu University, 55, 2, 261-268, 55, 2, 261-268, 2010.11. |
8. | Honjoh, K., Matsuura, K., Machida, T., Nishi, K., Nakao, M., Yano, T., Miyamoto, T., Iio, M., Enhancement of menadione stress tolerance in yeast by accumulation of hypotaurine and taurine: Co-expression of cDNA clones, from Cyprinus carpio, for cysteine dioxygenase and cysteine sulfinate decarboxylase in Saccharomyces cerevisiae, Amino Acids, 10.1007/s00726-009-0328-6., 38, 4, 1173-1183, 38(4): 1173-1183, 2010.04. |
9. | Machida, T., Ohashi, N., Mimura, A., Honjoh, K., Iio, M., Miyamoto, T., Chloroplastic glucose 6-phosphate dehydrogenase from Chlorella vulgaris alleviates freezing and menadione-indued oxidative stresses in Saccharomyces cerevisiae, Journal of the Faculty of Agriculture, Kyushu University , 55, 1, 29-38, 2010.03. |
10. | Machida, T., Honjoh, K., Shimizu, H., Yamamoto, M., Iio, M., Miyamoto, T., Expression of a gene encoding a functional glycosyl hydrolase, trehalase, from Nicotiana tabacum in Saccharomyces cerevisiae, Journal of the Faculty of Agriculture, Kyushu University , 54, 2, 297-304, 2009.11. |
11. | Machida, T., Kato, E., Ishibashi, A., Sato, J., Kawasaki, S., Niimura, Y., Honjoh, K., Miyamoto, T., Expression pattern of a chloroplast NADPH-dependent thioredoxin reductase in Chlorella vulgaris during hardening and its interaction with 2-Cys peroxiredoxin., Biosci. Biotechnol. Biochem., 73(3), 695-701, 2009.03. |
12. | Honjoh, K., Fujihara, K., Haraguchi, T., Ono, Y., Kobayashi, H., Hiwaki, H., Kamikado, H., Jang, S.S., Ryu, S., Miyamoto, T., Subtyping of Listeria monocytogenes based on nucleotide polymorphism in clpC, inlA, hlyA, and plcA genes and rapid identification of L. monocytogenes genetically similar to clinical isolates, Food Sci. Technol. Res., Vol. 14, No.6, 557-564, 2008.12. |
13. | Machida, T., Murase, H., Kato, E., Honjoh, K., Matsumoto, K., Miyamoto, T., Iio, M., Isolation of cDNAs for hardening-induced genes from Chlorella vulgaris by suppression subtractive hybridization., Plant Science, 175, 3, 238-246, 2008.09. |
14. | Machida, T., Kato, E., Ishibashi, A., Ohashi, N., Honjoh, K., Miyamoto, T., Molecular characterization of low-temperaure-inducible NTR-C in Chlorella vulgaris, Nucleic Acids Symposium Series, No. 51, 463-464, 2007.11. |
15. | Honjoh, K., Machida, T., Nishi, K., Matsuura, K., Soli, K.W., Sakai, T., Ishikawa, H., Matsumoto, K., Miyamoto, T., Iio, M., Improvement of freezing and oxidative stress tolerance in Saccharomyces cerevisiae by taurine, Food Sci. Technol. Res., 13,2, 145-154, 2007.06. ![]() |
16. | Honjoh,K., Machida, T., Hagisako, T., Suga, K., Yonekura, M., Shimizu, H., Ohashi, N., Miyamoto, T., Hatano, S., Iio, M., Molecular cloning and characterization of a cDNA for low-temperature inducible cytosolic glucose 6-phosphate dehydrogenase gene from Chlorella vulgaris and expression of the gene in Saccharomyces cerevisiae, Plant Science, 172, 649-658, 2007.03. ![]() |
17. | Honjoh, K., Mimura, A., Kuroiwa, E., Hagisako, T., Suga, K., Shimizu, H., Dubey, R.S., Miyamoto, T., Hatano, S., and Iio, M., Purification and characterization of two isoforms of glucose 6-phosphate dehydrogenase (G6PDH) from Chlorella vulgaris C-27, Biosci. Biotechnol. Biochem., 10.1271/bbb.67.1888, 67, 9, 1888-1896, 67(9): 1888-1896, 2003.09. ![]() |
18. | Honjoh, K., Suga, K., Shinohara, F., Maruyama, I., Miyamoto, T., Hatano, S., and Iio, M., Preparation of protoplasts from Chlorella vulgaris K-73122 and cell wall regeneration of protoplasts from C. vulgaris K-73122 and C-27, J. Fac. Agr., Kyushu Univ., 47, 2, 257-266, 47(2): 257-266, 2003.02. |
19. | Shimizu, H., Furuya, N., Suga, K., Honjoh, K., Miyamoto, T., Hatano, S., and Iio, M., Freezing tolerance of transgenic tobacco with increased content of unsaturated fatty acid by expressing the CvFad2 or CvFad3 gene, J. Fac. Agr., Kyushu Univ., 47, 2, 307-317, 47(2): 307-317, 2003.02. |
20. | Suga, K., Honjoh, K., Furuya, N., Shimizu, H., Nishi, K., Shinohara, F., Hirabaru, Y., Maruyama, I., Miyamoto, T., Hatano, S., and Iio, M.,, Two low-temperature-inducible Chlorella genes for D12 and w-3 fatty acid desaturase (FAD): Isolation of D12 and w-3 fad cDNA clones, expression of D12 fad in Saccharomyces cerevisiae , and expression of w-3 fad in Nicotiana tabacum, Biosci. Biotechnol. Biochem., 66, 6, 1314-1327, 66(6): 1314-1327, 2002.06. |
21. | Honjoh,K., Shimizu, H., Nagaishi, N., Matsumoto, H., Suga, K., Miyamoto, T., Iio, M., and Hatano, S., Improvement of freezing tolerance in transgenic tobacco leaves by expressing the hiC6 gene, Biosci. Biotechnol. Biochem., 10.1271/bbb.65.1796, 65, 8, 1796-1804, 65(8): 1796-1804, 2001.08. ![]() |
22. | Honjoh, K., Matsumoto, H., Shimizu, H., Ooyama, K., Tanaka, K., Oda, Y., Takata, R., Joh, T., Suga, K., Miyamoto, T., Iio, M. and Hatano, S., Cryoprotective activities of group3 late embryogenesis abundant proteins from Chlorella vulgaris C-27, Biosci. Biotechnol. Biochem., 10.1271/bbb.64.1656, 64, 8, 1656-1663, 64(8): 1656-1663, 2000.08. |
23. | K. Honjoh, Y. Oda, R. Takata, T. Miyamoto, and S. Hatano, Induction of the hiC6 gene, which encodes a homologue of a late embryogenesis abundant (LEA) protein, enhances freezing tolerance of yeast, Journal of Plant Physiology, Vol.155, No.4,5, 509-512, 1999.01. |
24. | Honjoh, K., Yoshimoto, M., Joh, T., Kajiwara, T., Miyamoto, T., Hatano, S., Isolation and characterization of hardening-induced proteins in Chlorella vulgaris C-27: Identification of late embryogenesis abundant proteins, Plant Cell Phyisiol., 36, 8, 1421-1430, 36(8), 1421-1430, 1995.12. |
25. | Joh, T., Honjoh, K., Yoshimoto, M., Funabashi, J., Miyamoto, T., Hatano, S., Molecular cloning and expression of hardening-induced genes in Chlorella vulgaris C-27: the most abundant clone encodes a late embryogenesis abundant protein, Plant Cell Physiol., 36, 1, 85-93, 36(1), 85-93, 1995.01. |
26. | Joh, T., Yoshimoto, M., Honjoh, K., Miyamoto, T., Hatano, S., Changes in translatable RNA population during hardening of Chlorella ellipsoidea C-27., J. Fac. Agr., Kyushu Univ., 37, 3-4, 257-263, 37(3,4), 257-263, 1993.02. |
Presentations
- Japanese Society for Safety Education
- Japanese Society for Plant Cell and Molecular Biology
- Japan Association of Food Preservation Science
- Japan Society for Bioscience, Biotechnology, and Agrochemistry
- The Japanese Society for Food Science and Technology
- The Japanese Society of Plant Physiologists
- Japanese Society of Food Microbiology


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