Kyushu University Academic Staff Educational and Research Activities Database
Researcher information (To researchers) Need Help? How to update
Naotaka YAMADA Last modified date:2020.06.11

Assistant Professor / Molecular Bioscience
Department of Bioscience and Biotechnology
Faculty of Agriculture

Graduate School
Undergraduate School

 Reseacher Profiling Tool Kyushu University Pure
Academic Degree
Doctor of Agliculture
Field of Specialization
bioorganic chemistry
Outline Activities
Development of new plant growth regurators and insect growth regulator
1.Synthesis of novel freezing- tolerance inducing agents for Chlorella vulgaris and research on its mode of action.
2.Development of new carotenoid biosynthesis inhibitors.
3.Search of New glutathione S-transferase with insecticides metabolic activity on Bombyx mori  

 lecture:organic chemistry, Natural products chemistry

Research Interests
  • Study on insecticide metabolism by Incect glutathione transferases (GST)  
    keyword : glutathione transferase,insecticide,
  • Physiological Analysis of 8-Nitro-cycli-GMP in Plant or Incet
    keyword : 8-Nitro-cycli-GMP
  • Rsearch of new bleaching compounds in targeting carotenoid biosynthesis in plant.
    keyword : bleaching, carotenoid, phetoene, zeta-carotene
  • Design, Synthesis and Biological Activity of Novel Anti-Juvenile Hormone Agents
    keyword : Juvenile Hormone, Insect growth regulator, organic synthesis
  • Isolation and identification of sucking deterrent against green rice leafhopper and brown planthopper
    in rice using Near Isogenic Lines.
    keyword : rice ・green rice leafhopper ・sucking deterrent ・Near Isogenic Lines
Academic Activities
1. Sumio Iwai, Sho Ogata, Naotaka Yamada, Michio Onjo, Kintake Sonoike, Ken ichiro Shimazaki, Guard cell photosynthesis is crucial in abscisic acid-induced stomatal closure, Plant Direct, 10.1002/pld3.137, 3, 5, e00137, 2019.05, Reactive oxygen species (ROS) are ubiquitous signaling molecules involved in diverse physiological processes, including stomatal closure. Photosynthetic electron transport (PET) is the main source of ROS generation in plants, but whether it functions in guard cell signaling remains unclear. Here, we assessed whether PET functions in abscisic acid (ABA) signaling in guard cells. ABA-elicited ROS were localized to guard cell chloroplasts in Arabidopsis thaliana, Commelina benghalensis, and Vicia faba in the light and abolished by the PET inhibitors 3-(3, 4-dichlorophenyl)-1, 1-dimethylurea and 2, 5-dibromo-3-methyl-6-isopropyl-p-benzoquinone. These inhibitors reduced ABA-induced stomatal closure in all three species, as well as in the NADPH oxidase-lacking mutant atrboh D/F. However, an NADPH oxidase inhibitor did not fully eliminate ABA-induced ROS in the chloroplasts, and ABA-induced ROS were still observed in the guard cell chloroplasts of atrboh D/F. This study demonstrates that ROS generated through PET act as signaling molecules in ABA-induced stomatal closure and that this occurs in concert with ROS derived through NADPH oxidase..
2. Kohji Yamamoto, Akifumi Higashiura, Aiko Hirowatari1, Naotaka Yamada,Takuya Tsubota, Hideki Sezutsu, Atsushi Nakagawa, Characterisation of a diazinonmetabolising glutathione Stransferase in the silkworm Bombyx mori by X-ray crystallography and genome editing analysis, Scientific REPORTS, 10.1038/s41598-018-35207-8, 8, 16835, 2018.11.
3. Aiko Hirowatari, Sumiharu Nagaoka, Naotaka YAMADA, KOHJI YAMAMOTO, Structural characterization of the catalytic site of a Nilaparvata lugens
delta-class glutathione transferase, Journal of Insect Biotechnology and Sericology, 86, 1-7, 2017.03.
4. KOHJI YAMAMOTO, Aiko Hirowatari, Takahiro Shiotsuki, Naotaka YAMADA, Biochemical characterization of an unclassified glutathione S-transferase of Plutella xylostella, Journal of Pesticide Science, 41, 4, 145-151, 2016.11.
5. Kenj Honda, Naotaka YAMADA, Riichiro Yoshida, Hideshi Ihara, Tomohiro Sawa, Takaaki Akaike, Sumio Iwai, 8-Mercapto-Cyclic GMP Mediates Hydrogen Sulfide-Induced Stomatal Closure in Arabidopsis, PLANT AND CELL PHYSIOLOGY, 10.1093/pcp/pcv069, 56, 8, 1481-1489, 2015.08.
6. KOHJI YAMAMOTO, Akifumi Higashiura, MD. Tofazzal Hossain, Naotaka YAMADA, Takahiro Shiotsuki, Atsushi Nakagawa, Structural characterization of the catalytic site of a Nilaparvata lugens
delta-class glutathione transferase, Archives of Biochemistry and Biophysics, 566, 36-42, 2015.01.
7. KOHJI YAMAMOTO, Yoichi Aso, Naotaka YAMADA, Catalytic function of an Epsilon-class glutathione S-transferase of the silkworm, Insect Molecular Biology , 10.1111, 22, 5, 523-531, 2013.06.
8. Takahiro Joudoi, Yudai Shichiri, Nobuto Kamizono, Takaaki Akaike, Tomohiro Sawa, Jun Yoshitake, 山田 直隆, Sumio Iwaia, Nitrated Cyclic GMP Modulates Guard Cell Signaling in ArabidopsisW, The Plant Cell, 10.1105, 25, 558-571, 2013.02.
9. Kenjiro Furuta, Norihiro Fujita, Tsubasa Ibushi, Takahiro Shiotsuki, Naotaka Yamada and Eiichi Kuwano, Synthesis and anti-juvenile hormone activity of ethyl 4-[(6-substituted 2,2-dimethyl-2H-chromen-7-yl)methoxy]benzoates
, Journal of Pesticide Science , 35, 4, 405-411 , 2010.05.
10. N. Yamada, E. Kuwano,, Synthesis and bleaching activity of 1-ethyl- and 1-propyl-5-substituted imidazoles(II), J. Fac. Agr.,Kyushu Univ., 46 (1), 219-228, 2001.10.
11. Yoshida, S., Furuta, K., Shirahashi, H., Ashibe, K., Fujita, N., Yamada. N , Kuwano. E, Synthesis and Structure-Activity Relationship of a New Series of Anti-Juvenile Hormone Agents: Alkyl 4-(2-Benzylhexyloxy)benzoates and Ethyl 4-Substituted Benzoates. , J.Fac.Agr.,Kyushu Univ.,, 54(1),179-184, 2009.01.
12. S. Hong, N. Yamada,A.Harada, S. Kawai and E. Kuwano, Inhibition of trans-Cinnamate 4-Hydroxylase by 4-amino-5aryl-2,3-dihydro-3H-1,2,4-triazole, J. Pestic. Science, 10.1584/jpestics.30.406, 30, 4, 406-408, 30(4), pp406-408, 2005.12.
13. Watanabe, Y., Yamada. N ., Machida, T., Honjoh, K., Kuwano,E. , Influence of Cold Hardening on Chlorophyll and Carotenoid in Chlorella vulgaris
, J.Fac.Agr.,Kyushu Univ.,, 54(1),195-200(2009), 2009.01.
14. Yoshida, S., Furuta, K., Ashibe, K., Fujita, N., Nishikawa, S., Yamada. N, Kuwano. E , Ethyl 4-[2-(Substituted Benzyl)hexyloxy]benzoates: Anti-Juvenile Hormone Agents with Juvenile Hormone Activity., J.Fac.Agr.,Kyushu Univ.,, 54(1),185-190(2009)
, 2009.01.
15. Naotaka Yamada, Daisuke Kusano,Eiich. Kuwano, Bleaching Activity of 4-Phenl-3-(substituted benzylthio)-4H-1,2,4-triazoles.Biosci.Biotech. and Biochem., 66(8) 1671-1676(2002), Bioscience Biotechnology and Biochemistry., (2002), 66, 8, 1671-1676, 66、8、pp1671-1676, 2002.08.
16. Naotaka Yamada, Shinya Kawai, Eiichi Kuwano 他3名, 5-Aryl-1,3,4-oxadiazole-2-thiols as a New Series of trans-Cinnamate, Journal of Pesticide Science, 10.1584/jpestics.29.205, 29, 3, 205-208, 29,3, pp205-208, 2004.03.
1. Role of hydrogen sulfide as a signaling material in induction of stomata closure of salicylic acid.
Naotaka Yamada, Daiki Shinboku, Sumio Iwai.
The 2021 Meeting of the Japan Society for Bioscience, Biotechnology and Agrochemistry..
2. Search for anti-ABA compounds by using ABA agonist, Quinabactin as a lead compound
Kohei Taksugi, Yushiro Fukusaki, Naotaka Yamada (Faculty of agriculture, Kyushu Univ.),
45th Annual Meeting of the Pesticide Science Society of Japan.
3. Biochemical analysis and crystal structure analysis using specific inhibitor Z302 of basidiomytesal yeast and plant-derived phenylalanine ammonia lyase.
Takashi Nakashima, Takahiro Yoshimura, Daiki Tomitaka, Naotaka Yamada, Yoshizumi Ishino.
The 2019 Joint Meeting of The West and Chushikoku Branch of the Japan Society for Bioscience, Biotechnology and Agrochemistry. .
4. Changes in H2S, NO and H2O2 levels in guard cells during the guard closure induction by salicylic acid,
Naotaka Yamada, Daiki Shinboku, Sumio Iwai.
The 2019 Joint Meeting of The West and Chushikoku Branch of the Japan Society for Bioscience, Biotechnology and Agrochemistry..
Educational Activities
School of agriculture
  :Lecture of Organic ChemistryI
   :Laboratory Course of Organic Chemistry ( Applied Bioscience course)

Graduate school
: Proseminar in Peticide Chemistry and Organic Synthesis I and II, Advanced Studies of Plant Protection I and II (Applied Genetics and Pest Management)