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kuniyoshi shimizu Last modified date：2023.04.17

Associate Professor / Systematic Forest and Forest Products Science
Department of Agro-environmental Sciences
Faculty of Agriculture


Graduate School
Department of Agro-environmental Sciences
Graduate School of Bioresource and Bioenvironmental Sciences
Department of Forest and Forest Products Sciences
Graduate School of Bioresource and Bioenvironmental Sciences


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Homepage
https://kyushu-u.pure.elsevier.com/en/persons/kuniyoshi-shimizu
　Reseacher Profiling Tool　Kyushu University Pure
https://www.agr.kyushu-u.ac.jp/lab/shinrinken/index.html
Laboratory of Systematic Forest and Forest Products Sciences .
http://ffpsc.agr.kyushu-u.ac.jp/sffps/　　　
Homepage of Laboratory of Systematic Forest and Forest Products Sciences.
http://shinrinken.main.jp/ .
Phone
092-642-3002
Fax
092-642-3002
Academic Degree
PhD
Country of degree conferring institution (Overseas)
No
Field of Specialization
Natural Products Bioorganic Chemistry
Total Priod of education and research career in the foreign country
02years00months
Outline Activities
The investigation of LOHAS-oriented biological activity of forest products and their applications

Forest has been known to have healing power for mind and body. Our group deals with LOHAS (Life Of Health And Sustainability) oriented biological aspects of forest products and their mode of action. In most cases this involves investigating the biological activity of extractives of mushroom , woods and plant etc, such as anti-androgenic activity, prevention effect of bone loss in calcium deficient ovariectomized rats of mushroom, healing aroma, and melanin biosynthesis inhibitory activity of wood extractives. Our research cover several practical fields such as cosmetics, aroma, functional food, living environment, toiletry etc.

1, Search for biologically active substance from plants.
2, Search for biologically active substance from mushrooms.
3, Studies on the mode of action of bioactive natural substances and their structural criteria.
4, The physiological and psychological effect of aroma (natural volatiles) on human.
5, Development of bioactive substances and extracts for practical use (cosmetics, aroma, functional food, toiletry and living environment.)
Research
Research Interests
  • Evaluation of the functional of Angelica keiskei
    keyword : Angelica keiskei　Evaluation of the functional
    2019.10.
  • Functional evaluation of honey and isolation and identification of compounds
    keyword : Honey　Isolation and identification　Functional evaluation
    2020.10.
  • Isolation and Identification of Compounds in AKAJISO(Perilla frutescens var. crispa f. purpurea) and Functional evaluation
    keyword : AKAJISO(Perilla frutescens var. crispa f. purpurea)　Isolation and Identification　Functional evaluation
    2018.04.
  • Establishment of functional evaluation and analysis methods for argan saponins
    keyword : Argan Argan oil Argan saponins
    2020.06.
  • In vitro functional evaluation test of ethanol and water extract of Houttuynia cordata
    keyword : Houttuynia cordata
    2019.09～2020.02.
  • Functional components isolated from Olive
    keyword : Olive
    2013.04.
  • Verification of effects on human cognitive function using foods containing peanut extract
    keyword : Arachis hypogaea
    2019.09～2020.03.
  • A supplement containing Nejimebiwa tea as a functionally labeled food that contributes to the suppression of allergic symptoms
    keyword : Eriobotrya japonica
    2019.07～2020.02.
  • Basic research on improvement of cognitive function of ingredients containing Yamabushitake
    keyword : Hericium erinaceus
    2019.04～2020.03.
  • Volatile compounds emitted from Juncus effusus and their functions
    keyword : Juncus effusus, Igusa,GC/MS,Tatami
    2018.04～2021.03.
  • Comparison of the physiological and psychological response of the component profile and human essential oil aroma prepared from cedar leaves and wood parts
    keyword : Cryptomeria japonica essential oil
    2013.04～2015.02.
  • Wild Mushroom in Nepal: Some potential Candidates as Antioxidant and ACE Inhibition Sources
    keyword : Wild Mushroom in Nepal
    2013.04.
  • Comparative Biological Study of Roots, Stems, Leaves, and Seeds of
    Angelica shikokiana Makino

    keyword : Angelica shikokiana
    2013.04.
  • Search for bioactive compound from the waste of Ejyptian plants
    keyword : Medicinal Plants from Ejypt
    2013.04.
  • HMGCoA reductase inhibitors from natural resources
    keyword : HMGCoA reductase inhibitor
    2008.05～2010.03.
  • Search for hair growth agents from natural products
    keyword : hair growth
    2008.05～2010.03.
  • Functional components isolated from Eriobotrya japonica
    keyword : Eriobotrya japonica
    2008.05.
  • Search for bioactive compounds from the bamboo and the skin of bamboo shoot
    keyword : bamboo shoot, biological activity
    2007.05.
  • Volatile compounds emitted from woods and their functions
    keyword : terpene, volatile
    2006.04.
  • The physiological and psychological effects of volatiles emitted from natural resources on human
    keyword : natural product, volatile, aroma, essential oil
    2005.04.
  • The aldose reductase inhibitors from Ganoderma lucidum
    keyword : aldose reductase, Ganoderma lucidum
    2004.04The inhibitory activity of the ethanol extract of Ganoderma lucidum on aldose reductase..
  • Search for the preventive compounds from mushroom on benign prostate hyperplasia
    keyword : Ganoderma lucidum, fruiting body, antiandrogen, 5a-reductase
    2001.04Screening of the inhibitors of benign prostatic hyperplasia from edible and medicinal mushrooms..
  • Search for the compound with estrogenic activity from mushrooms
    keyword : fruiting body, estrogenic activity, Pleurotus eryngii, Ganoderma lucidum
    2002.04Screening of the prophylactic compounds for osteoporosis from edible and medicinal mushrooms..
Academic Activities
Books
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1. I. Kubo, T. J. Ha, K. Shimizu, Molecular Design of Soybean Lipoxygenase Inhibitors Based on Natural Products, A Comprehensive Survey of International Soybean Research - Genetics, Physiology, Agronomy and Nitrogen Relationships, InTech, James E. Board (Ed.)
DOI: 10.5772/52703., 2013.03, [URL].
2. N. Masuoka, K. Shimizu, I. Kubo, Antioxidant Activity of Anacardic Acids
In: Antioxidants and Biocides from Latin American Plants;
, CABI Publishing (UK), C.L. Cespedes, D.A. Sampietro, D.S. Seigler, Ed., 2012.11.
3. I. Kubo, K. Fujita, K. Shimizu, Anti-Salmonella Agents from Tanacetum Balsamita, A Brazilian Medicinal Plant, and their Application
In: Antioxidants and Biocides from Latin American Plants, CABI Publishing (UK), C.L. Cespedes, D.A. Sampietro, D.S. Seigler, Ed., 2012.11.
4. I. Kubo, K. Shimizu, K. Nihei, MANICOBA, A quercetin rich Amazonian dish
In: Quercetin: Dietary Sources, Functions and Health Benefits, Nova Science Publishers, Inc., 2012.11.
5. I. Kubo, K. Shimizu, K. Fujita, Naturally Occurring Antifungal Agents and Their Modes of Action.
Fungicides for Plant and Animal Diseases, InTech, D. Dhanasekaran, N. Thajuddin and A. Panneerselvam (Ed.), 2012.11, [URL].
Reports
 Show All Reports >>
1. Amira Mira, kuniyoshi shimizu, An Update on Antitumor Activity of Angelica Species, Medicinal Plants - Recent Advances in Research and Development, pp 363-371, 2016.10.
2. I. Kubo, T. J. Ha, K. Shimizu, Molecular Design of Soybean Lipoxygenase Inhibitors Based on Natural Products, A Comprehensive Survey of International Soybean Research - Genetics, Physiology, Agronomy and Nitrogen Relationships, James E. Board (Ed.), ISBN: 978-953-51-0876-4, InTech, DOI: 10.5772/52703., 2013.01.
3. N. Masuoka, K. Shimizu, I. Kubo, Antioxidant Activity of Anacardic Acids
, In: Antioxidants and Biocides from Latin American Plants; C.L. Cespedes, D.A. Sampietro, D.S. Seigler, Ed., CABI Publishing (UK)(2012), In: Antioxidants and Biocides from Latin American Plants;
C.L. Cespedes, D.A. Sampietro, D.S. Seigler, Ed., CABI Publishing (UK)(2012), 2012.02.
4. I. Kubo, K. Fujita, K. Shimizu, Anti-Salmonella Agents from Tanacetum Balsamita, A Brazilian Medicinal Plant, and their Application, In: Antioxidants and Biocides from Latin American Plants; C.L. Cespedes, D.A. Sampietro, D.S. Seigler, Ed., CABI Publishing (UK), 2012.02.
5. I. Kubo, K. Shimizu, K. Nihei, MANICOBA, A quercetin rich Amazonian dish, In: Quercetin: Dietary Sources, Functions and Health Benefits- Nova Science Publishers, Inc., 2012.02.
6. I. Kubo, K. Shimizu, K. Fujita, Naturally Occurring Antifungal Agents and Their Modes of Action., In : Fungicides Volume I; D. Dhanasekaran, N. Thajuddin, A. Panneerselvam, Ed., InTech - Open Access Publisher, 2012.02.
7. I. Kubo, N. Masuoka, T. J. Ha, K. Shimizu, K. Nihei, Multifunctional Antioxidant Activities of Alkyl Gallates, The Open Bioactive Compounds Journal, 3, 1-11(2010), 2010.06.
8. I. Kubo, T. J. Ha, T. Nitoda, K. Shimizu, A. Kubo, Multifunctional Acne Control Agents , Current Topics in Phytochemistry, 9, 95-102 (2008) , 2008.06.
9. K. Shimizu, Tyrosinase and 5a-Reductase Inhibitory Components from Artocarpus incisus, Bull. Kyushu Univ. For., 82, p21-63, 2001.01.
10. K. Shimizu, R. Kondo, K. Sakai, Tyrosinase inhibitors from Artocarpus incisus., Research Advances in Phytochemistry, 1, p19-34, 2000.01.
Papers
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Presentations
 Show All Presentations >>
1. Kuniyoshi Shimizu, Multi-medicinal mushroom Ganoderma lingzhi, Ethnopharmacology: Globalization of Traditional Medicine, 2023.03.
2. Mittraphab Yanisa, Kuniyoshi Shimizu, Anti-phototoxicity of Phenolic compounds from Entada phaseoloides leaves in Human Epidermal Keratinocytes, 11th Asian Natural Product Conference (Q-AOS), 2022.06.
3. Ahmed Othman, Kuniyoshi Shimizu, Possible neuroprotective effect of amide alkaloids from some halophytes growing in Egypt, 11th Asian Natural Product Conference (Q-AOS), 2022.06.
4. Kuniyoshi Shimizu, Ganoderma Triterpenoids: Hint for Understanding Multi-Medicinal effects of Ganoderma mushroom, International Congress of SOCIETY FOR ETHNOPHARMACOLOGY, INDIA 2022, 2022.04, Lingzhi (in Chinese) or Reishi (in Japanese), recently identified as Ganoderma lingzhi which is different from Ganoderma lucidum originally described from Europe, has been treasured for its medicinal value in traditional medicine in Japan, China and Korea for thousands of year. Of all Ganoderma mushrooms, which account for more than 300 known triterpenoids, G. lingzhi has been the most well-studied species. G. lingzhi was reported with a wide range of bioactivities including antioxidative, anti-aging, immunomodulatory activity, anti-hepatotoxic, ant-angiogenic, anti-histaminic, potential anticancer activity and even anti-HIV. Our group has isolated many triterpenoids from reishi and reported their bioactivities, including inhibition of benign prostatic hypertrophy, anti-androgenic osteoclastogenesis, anti-diabetes, anti-diabetic complication, antihypertension, inhibition of neuraminidase and anti-cancer activities. Following topics will be discussed.
1) Mushroom sample and triterpenoids isolation
2) 5-reductase inhibition and anti-benign prostate hyperplasia
3) Inhibition of osteoclast differentiation (anti-osteoporosis)
4) Inhibition of α-glucosidase (anti-diabetes)
5) Inhibition of aldose reductase (anti-diabetic complication)
6) Inhibition of angiotensin-converting enzyme (anti-hypertension)
7) Inhibition of neuraminidase (anti-influenza)
8) Inhibition of Rho kinase
9) Tumor cell cytotoxicity (anti-cancer)
10) Clues to pharmacological mechanisms (identification of target protein)
11) LC-MS/MS analysis for evaluating functional-quality of Ganoderma mushroom

Up to now, more than 300 triterpenoids have been reported from Ganoderma mushroom, especially from G. lingzhi. Also, many bioactivities of these compounds have been investigated. Potential application of this mushroom and the compounds in it on three indications including cancer, diabetes, and immune stimulation was also thoroughly examined. However, future randomized controlled clinical trials are still necessary. Despite of lacking of enough evidence from clinical trials, this mushroom remains a popular agent in commercial products. From this fact, studies on the Ganoderma mushroom should be still a hotter topic in the near futures.
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5. Kuniyoshi Shimizu, Utilization of natural resources based on scientific evidence for sustainable development-pharmaceutical, functional food, aroma, living environment, Tunisia-Japan Symposium-Materials for Sustainable Development: Fundamentals & Applications, 2022.03.
6. Ran,Suzuki,Wang Dongmei,Yhiya Amen,Naomichi Takemoto,Fatima Ezzahra Janati Idrissi,Tarik Ouchbani,Mitsutoshi Nakajima, Hiroko Isoda,Kuniyoshi Shimizu, An analytical method for argan saponins in argan oil by using liquid chromatography quadrupole time-of-flight mass spectrometry, Tunisia-Japan Symposium-Materials for Sustainable Development: Fundamentals & Applications , 2022.03.
7. Kuniyoshi SHIMIZU, Utilization of natural resources for sustainable development based on scientific evidence-Use for pharmaceuticals, functional foods, aromas, living environment-, 1st International Conference on Highland Germpalsm (ICHG) -Technological Innovation and Sustainable Development Based on Highland Germplasm-, 2022.02.
8. Kuniyoshi SHIMIZU, How should we utilize natural resources for sustainable development based on scientific evidence?-Use for pharmaceuticals, functional foods, aromas, living environment-, International Conference-Natural Resources Governance Toward Sustainable Development, 2021.11.
9. Thien Huu Nguyen, Yhiya Amen, Dongmei Wang, Ahmed Othman, Kuniyoshi Shimizu, Characterization and Kinetic Studies of Proanthocyanidin Oligomers Complex Derived from Avocado Seed As a Promising Antidiabetic Agent, 8th Asian Natural Product Conference (Q-AOS), 2021.10.
10. Asmaa Kamal El-Deen, Kuniyoshi Shimizu, Suspect and Non-target Screening of Emerging Contaminants in Wastewater Using LC-QTOF/MS in Data Independent Acquisition (DIA) Mode: Occurrence, Fate and Risk Assessment, 8th Asian Natural Product Conference (Q-AOS), 2021.10.
11. Duanyang Wang, Maki Nagata, Masako Matsumoto, Yhiya Amen, Dongmei Wang, Kuniyoshi Shimizu, Bio-Activity Evaluation of Hibiscus sabdariffa Linn. in Skin Care and Isolation of Contributory Component, Kyushu University Asia Week 2021, 2021.10.
12. Yanisa Mittrapha, Maki Nagata, Massako Matsumoto, Kuniyoshi Shimizu , Protective effects of acetone extract from Entada phaseoloides leaves on UVB-irradiated HaCaT keratinocytes, Kyushu University Asia Week 2021, 2021.10.
13. Ruan Yang, Yanisa Mittraphab, Ahmed Muhamed Othman, Yhiya Amen, Wang Dongmei, Han Chunguang, Mika Abe, Kuniyoshi Shimizu , The Isolation and Identification of Hericenones in Hericium erinaceus (Yamabushitake) , Kyushu University Asia Week 2021, 2021.10.
14. Ahmed Othman, Yhiya Amin, Masako Matsumoto, Maki Nagata, Kuniyoshi Shimizu, Utilization of Halophytes as a Source of Pharmaceutical Candidates, Kyushu University Asia Week 2021, 2021.10.
15. Jiaxin Fan,Ahmed Ashour, Ahmed Hassan, Yhiya Amen, Maki Nagata, Masako Matsumoto, Shuhei Kaneko, Dongmen Wang, Satoshi Ishikawa, Shinich Muta, Kuniyoshi Shimizu, The expansion of the promising value of Flammulina velutipes and the prospect of its sustainable development, Kyushu University Asia Week 2021, 2021.10.
16. Asmaa Kamel El-Deen,Kuniyoshi Shimizu, Suspect and non-target screening of emerging contaminants in wastewater using LC-QTOF/MS: An insight on occurrence, fate, risk, and wastewater management in Asia, Kyushu University Asia Week 2021, 2021.10.
17. Kuniyoshi SHIMIZU, How do we utilize natural resources based on scientific evidence? -Use for pharmaceuticals, functional foods, cosmetics, aromas, and living environment-, International Conference on Natural Sciences, Technology, Islamic Studies, Social Sciences and Humanity (ICoNTISH), 2021.11.
18. 清水邦義, How do we utilize natural materials based on scientific evidence? -Use for pharmaceuticals, functional foods, cosmetics, aromas, and living environment-, The 2nd International Conference Series on Biodiversity Status Update of North Borneo, 2021.09.
19. 清水邦義, How to utilize natural products as the valuable products -Medicine, Functional Food, aroma, Woody house, Academic Seminar International Conference (2ND ASIC), 2021.08.
20. 清水邦義, Lifestyles of Health and Sustainability (LOHAS) concept for the better future: How to utilize natural products as the valuable products -Medicine, Functional Food, aroma,
Woody house, New Challenges in Pharmaceutical Research and Education in Egypt, 2021.03.
21. Taisuke Nakashima, Akiyoshi Honden, Minkai Sun, Yuri Yoshimura, Toshinori Nakagawa, Hiroya Ishikawa, Jun Nagano, Yuki Yamada, Tsuyoshi Okamoto, Yuichiro Watanabe, Shinji Yasunari, Koichiro Ohnuki, Noboru Fujimoto, Kuniyoshi Shimizu. , Effects of Japanese cedar scent on the human brain function, Asian Natural Product Conference (Q-AOS), 2020.05.
22. Rogers Mwakalukwa, Kuniyoshi Shimizu, Cultivar-specific metabolic profiling of olive leaves cultivars by LCMS based non-targeted metabolomics for the determination of AChE inhibitor metabolites , Asian Natural Product Conference (Q-AOS), 2020.05.
23. Yunfei XIAO，Yhiya AMEN，Asmaa KAMAL EL-DEEN，Naomichi TAKEMOTO，Dongmei WANG，Masako MATSUMOTO，Maki NAGATA，Kenshin HIROMATSU，Kuniyoshi SHIMIZU,
Isolation, Characterization and in Vitro Antioxidant Activity of Polysaccharide from Pholiota adiposa, 第70回日本木材学会, 2020.03, Pholiota adiposa is a species of edible fungus in the family Strophariaceae. It is a valuable mushroom that is grown only in Fukuoka. Polysaccharides extracted from P. adiposa were reported to have immune-stimulatory and antitumor properties, as well as hypolipidemic effect, anti-oxidant and anti-aging activities. We decide to conduct a more systematically studies of P. adiposa Polysaccharide (PAP), including the extraction, isolation, purification, characterization and in vitro antioxidant activity of purified polysaccharide. Crude polysaccharide of P. adiposa was extracted by hot water extraction and alcohol precipitation, and deproteinized by Sevag method. The crude polysaccharide of P. adiposa was further purified using Diethylaminoethanol (DEAE) Sepharose FF and Sephacryl S-200 HR column chromatography. After UV-visible spectrophotometry and gel permeation chromatography (GPC) analysis, the separated polysaccharide was determined to be a purified polysaccharide of 95% and 99% purity. The molecular weight was determined using pullulan standards (molecular weight T-736, 348, 200, 113, 48.8, 23.0, 9.9, 6.6 KD) and the monosaccharide composition was analyzed by HPLC-ELSD. The isolates were analyzed by Fourier Transform Infrared Spectroscopy (FT-IR), Nuclear Magnetic Resonance(NMR) for structural analysis. Then, the antioxidant ability of the polysaccharide fractions was evaluated by the ORAC method and the DPPH method. .
24. YHIYA Amen，AHMED Ashour，ROGERS Mwakalukwa，NAOMICHI Takemoto，MASUMI Nagae，KENSHIN Hiromatsu，KOICHIRO Ohnuki，KUNIYOSHI Shimizu, Anti-Hyperglycemic Effect of Pholiota adiposa on Human Subjects and isolation of active components, 第70回日本木材学会, 2020.03, Mushrooms are known to possess bioactive compounds responsible for anti-hyperglycemic effect. An in vitro α-glucosidase inhibition assay of 8 different mushroom species, resulted in the recognition of the methanolic extract of Pholiota adiposa as the strongest inhibitor. A randomized placebo controlled clinical trials performed in 15 healthy Japanese adults, showed significant dose dependent decrease in the blood glucose level, after consumption of 25 g and 50 g fresh equivalent of P. adiposa. Deep investigation of the methanolic extract of P. adipose, guided by an in vitro α-glucosidase inhibitory assay was performed for isolation of the active components. The n-hexane-soluble fraction of the methanolic extract was found active and lipids-like constituents of this fraction were found to be responsible for the activity of the extract. These results will be valuable in establishing P. adiposa as an important source of bioactive compounds capable of suppressing postprandial hyperglycemia in patients with Type 2 diabetes..
25. MWAKALUKWA Rogers，AMEN Yhiya，SHIMIZU Kuniyoshi, Postprandial hyperglycemia lowering effect of metabolites isolated from olive mill wastes (OMW)―An inhibitory activity on α-glucosidase and α-amylase enzymes―, 第70回日本木材学会, 2020.03, Diabetes is characterized by chronic hyperglycemia resulting from either, when the pancreas does not produce enough insulin (type 1), or when the body cannot effectively use the insulin it produces (type 2). The raise of blood glucose level, postprandially, results from hydrolysis of carbohydrates catalyzed by α-glucosidase and α-amylase. Thus, inhibition of these enzymes results into massive reduction of postprandial blood glucose levels ? a major cause of diabetes. In the present study, we report the isolation and anti-diabetic activity (inhibitory effect on α-glucosidase and α-lipase) of eight (8) compounds, one of them being novel, from olive mill wastes (OMW). Additionally, we investigated the enzyme kinetic analysis of the most active compounds to assist the possible characterization of the inhibition mechanisms involved. We observed that four compounds, 1, 2, 4 and 7, had higher activity against both enzymes. The kinetics studies of these compounds revealed that oleanolic acid 1 and luteolin-7-O-glucoside 7 inhibit α-glucosidase in a mixed-type fashion, while 1-acetoxypinoresinol 4 does it uncompetitively. Both mixed type and uncompetitive inhibition are similar to noncompetitive inhibition except that binding of the substrate or the inhibitor affect the enzyme’s binding affinity for the other. Thus for both, mixed type and uncompetitive, Ki value, which measures the affinity of the inhibitor for an enzyme was greater than 1 (Ki >1). Even though the novel compound, 3,4-dihydroxyphenyl-2-ethoxyethanol 5 exhibited poor activity, it helped to study the SAR for compounds of same class (hydroxytyrosol acetate 6 and hydroxytyrosol), whereby we clearly observed that both methoxylation and esterification of different positions does not influence their activity against the enzymes. This work attracts more attention to OMW as a potential source of lead compounds for structural modification to improve and develop new anti-diabetic drugs..
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  • 2022.11.


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