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Ozaki Shogo Last modified date:2021.12.14

Undergraduate School

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 Reseacher Profiling Tool Kyushu University Pure
Academic Degree
Country of degree conferring institution (Overseas)
Field of Specialization
Molecular biology, Microbiology, Genetics
Total Priod of education and research career in the foreign country
Outline Activities
Growing bacteria require careful tuning of cell division processes with dynamic organization of replicating chromosomes. The objective of our project is to understand molecular mechanisms for coordination of chromosome replication with division. To achieve this we make use of different model bacteria including Escherichia coli, Caulobacter crescentus, and Thermotoga maritima.
Research Interests
  • Mechanism and regulation for initiation complex that promotes local duplex DNA unwinding at replication origin during the initiation of chromosomal replication
    keyword : DNA replication, cell cycle, DnaA, AAA+, conformational change
Academic Activities
1. Katayama, T, Ozaki, S, Keyamura, K, Fujimitsu, K., Regulation of the replication cycle: conserved and diverse regulatory systems for DnaA and oriC, Nat Rev Microbiol, 2010, 2010.03.
2. Ozaki S., Katayama T., DnaA structure, function, and dynamics in the initiation at the chromosomal origin., Plasmid, 2009.07.
1. Shogo Ozaki,Tsutomu Katayama, Analysis of the chromosomal replication mechanism in the eubacterium Caulobacter crescentus, 日本分子生物学会第44回大会, 2021.12.
2. Shogo Ozaki、Yasutaka Wakasugi、Urs Jenal, Tsutomu Katayama, A novel divisome-associated protein spatially couples the Z-ring with the chromosomal replication terminus in Caulobacter crescentus, CAULOCONFERENCE 2020, 2020.04.
3. 尾崎 省吾, 若杉泰敬, 片山 勉, Identification and characterization of a novel DNA binding protein that colocalizes the chromosome replication terminus with cell division apparatus in Caulobacter crescentus, 第42回日本分子生物学会年会, 2019.12, The bacterial chromosomes are spatially and timely organized to execute chromoso
me replication and segregation in a manner coupled to cell division. In the gram
-negative, alphaproteobacterium Caulobacter crescentus, subcellular posit
ioning of the individual chromosomal loci alters dynamically during chromosome r
eplication. In a pre-replication stage, the origin of chromosome replication is
sequestered to one pole of the cell while the terminus region is localized at th
e other cell pole. Upon initiation of chromosome replication, one of the newly-r
eplicated origin DNAs is driven toward the other cell pole to establish bipolar
localization of the origin DNAs. Concomitantly, the terminus region is recruited
to the midcell position where the bacterial tubulin homolog FtsZ colocalizes to
form cytokinetic Z-rings. Although this colocalization likely coordinates cell
division with dynamic chromosome architecture, the molecular basis for a physica
l link between the Z-rings and the terminus region remains unknown. In this stud
y, we identify the terminus-interactive DNA binding protein ZapT and show that i
t directly binds to a component associated with the Z-rings. Moreover, the za
gene is crucial to maintain normal cell division control in C. crescentus
. Together, we propose that ZapT acts as a molecular bridge that physically link
s the terminus region to the Z-rings, thereby ensuring precise processes in chro
mosome segregation and cell division. Because ZapT orthologs are conserved among
diverse proteobacterial species, our findings may represent a general mechanism
to coordinate cell division with chromosome organization in time and space..
4. Shogo Ozaki, Lori Christian, Urs Jenal, The second messenger signaling drives chromosome replication in the asymmetrically dividing bacterium Caulobacter crescentus, 第56回日本生物物理学会年会, 2018.09.
5. Analysis on the minimal functional structure of the DnaA complex for the regulation of duplex DNA unwinding.
6. Analysis on the minimal functional elements of the replication origin in duplex DNA unwinding by DnaA.
Membership in Academic Society
  • American Society for Microbiology
  • The Japanese Biochemical Society
  • The Genetics Society of Japan
  • The Molecular Biology Society of Japan
  • Best Papers Awards in the 80th Annual Meeting of the Genetic Society of Japan
  • Best Papers Awards in the 79th Annual Meeting of the Genetic Society of Japan