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Yoshiko Miura Last modified date:2024.04.25

Professor / Department of Chemical Systems and Engineering, Graduate School of Engineering, Graducate School of Engineering,
Department of Chemical Engineering
Faculty of Engineering

Graduate School
Undergraduate School

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Web site of Lab 9, Department of Chemical Engineering, Kyushu University .
Academic Degree
Doctor of Engineering, Kyoto University,2000, Master of Engineering, Kyoto University, 1997, Bachelor of Engineering, Kyoto University, 1995
Country of degree conferring institution (Overseas)
Yes Bachelor Master Doctor
Field of Specialization
Biopolymers, Functional Polymer, Polymer Synthesis、Chemical Engineering
ORCID(Open Researcher and Contributor ID)
Total Priod of education and research career in the foreign country
Outline Activities
[ Research Activities]
Based on polymeric materials, chemical engineering, biopolymers, and interface science, I am conducting the following research.
(1)Biounctional materials with glycopolymers
Glycans on the cell surface act as signal molecules for life. Polymers with these attached to their side chains are polymeric materials that emit the molecular recognition ability of glycans. By designing sugar chains in various ways, it is possible to create polymers that efficiently cause recognition of various biomolecules such as cells, viruses, and toxin proteins.
In the synthesis of glycopolymers, vinyl compounds to which sugar chains are attached can be linked to precise materials and composite materials using polymer synthesis chemistry. Especially in recent years, precision controlled polymerization such as living radical polymerization has been used to design precise binding to proteins and cells using precision polymers such as block polymers, star polymers, and nanogels. We are also developing syntheses that incorporate the latest polymerization, photopolymerization. By graft polymerization in combination with base materials, bio-recognitive materials and devices can be created, which can be used for materials to eliminate specific pathogens. It is also possible to develop molecules for controlling cellular immunity. In our group we are currently using these technologies to develop materials useful for medical devices and basic biology.

(2) Immobilized catalysts and new chemical synthesis processes using porous polymer materials
Immobilized catalysts are important in chemical processes, including organic synthesis, because of the easy separation of catalyst and substrate and their applicability to distribution processes. On the other hand, in the organic synthesis processes, immobilized catalysts have disadvantages in mass transfer compared to homogeneous catalysts. Therefore, in our group we are developing immobilized catalysts using porous materials that facilitate substrate mass transfer and have a large effective surface area. Porous polymer immobilized catalysts are prepared by microphase separation phenomena of polymers. Organometallic and molecular catalysts are used as catalysts. Porous polymers have high solution permeability, and accordingly, the mass transfer of substrates is good. In particular, we are investigating continuous- flow synthesis using porous polymer-immobilized catalysts.

(3) Polymer Functional Materials by Machine Learning
Polymers are a major component of soft matter and have a wide range of applications in chemistry, biotechnology, and materials. They are also important carbon neutral targets. The development of soft matter is a complex process that requires a great deal of human capital and time. This approach will be greatly modified to develop polymeric functional materials using methods that quickly optimize experimental results. The problem is how to data polymeric materials. Based on the method of developing functional polymers with copolymers that has been conducted in our laboratory, we are proceeding with learning using monomer ratios and the development of functional materials through this method. Through the research described in (2) above, we are also studying chemical processes that can lead to automated synthesis using flow synthesis.

[Educational Activities]
Research guidance and education for undergraduate graduate research students, doctoral students, and master's students are conducted. In undergraduate courses, I am in charge of Organic Material Chemistry (all undergraduate courses), Physical Chemistry I (second-year students), and Reaction Engineering (third-year students), and Bio-resource Materials Engineering (graduate school) In the laboratory, For graduate and undergraduate students, the program provides individualized instruction in experiments, reading and writing papers, and training in logical thinking. In addition to the research aspect, guidance is provided on daily life in cooperation with their families if needed. For undergraduate and graduate students, we promote and provide guidance for presentations at domestic and international academic conferences. The department participates in university-wide and departmental FD meetings every year to improve education and teaching methods.

[International collaborative activities]
International joint research and exchanges with overseas researchers are conducted through international conferences. We have been conducting joint research with the University of Pennsylvania, California University (Irvine), University of Alberta, and Kyungpook National University (Korea). International collaborative activities in the fields of glycopolymers are conducted through international conferences.
She is also an Associate Editor of Journal of Material Chemistry B, Materials Advances and Chemistry Letters,and an Editorial Board member of ACS Macro Lett, ACS Applied He also serves on the editorial boards of Membrane and Applied Science. She also serves on the Editorial Board of Membrane and Applied Science, and is a reviewer for many international journals.

[Social Activities]
I belong to the Society of Polymer Science, the Chemical Society of Japan, the Society of Chemical Engineers, the Society of Carbohydrate Research, the Japan MRS, and the American Chemical Society. I am the chairperson of the Gender Equality Committee of the Chemical Society of Japan and a member of the Gender Equality Committee of the Japan Carbohydrate Society. I am the Kyushu Branch Secretary of the Society of Polymer Science and Technology. I served as the planning secretary for the Kyushu branch of the Society of Chemical Engineers. As a member of the Third Division of the Science Council of Japan, I work to promote Japanese science with a focus on chemistry, and am active in subcommittees on polymer chemistry, chemical engineering, and biomaterials. Serves as area advisor for JST and Act-X. I serves as an expert member of the Cabinet Office Food Safety Commission. As for foundation activities, it reviews academic support activities and scholarship programs for the Maki Tobe Foundation and the Nakatani Foundation.

[University Administration].
I serve as a member of the Faculty of Engineering Steering Committee, Faculty of Engineering Education Planning Committee, and Faculty of Engineering Experimental Ethics Committee. In addition, I have been in charge of the chemical engineering department, department head, vice department head, homeroom teacher for each grade, fall and spring factory tours, engineering department education planning, research planning, finance committee, human resources committee, central analysis committee, etc. are doing. I am a member of the Harassment Committee for the Ito District of Kyushu University. In the past, I served as a member of the Gender Equality Committee. In addition, I handles university and graduate school entrance examinations and cooperates with university and department operations.
Research Interests
  • 4D Gel by PET-RAFT polymerization
    keyword : 4D materials, Hydrogel, PET-RAFT polymerization
  • Polymer Functional Materials by Machine Learning
    keyword : Polymer, Soft Materials, Machine Learning, Bayesian inference
  • Development of Biofunctional Polymer Library Using Oxygen Tolerant Polymerization
    keyword : Oxygen tolerant polymerization, Biofunctional polymers, PET-RAFT, glycopolymer
  • Immobilized catalyst with porous polymers
    keyword : Immoblized catalyst, porous polymer, flow synthetic process
  • Separation Membrane with polymer monolith
    keyword : Porous polymer, polymer monolith
  • Development of Oligosaccharide mimic with glyco-module method
    keyword : Saccharide、Module, Glycopolymer, SPR
  • Separation of Biomolecules with metal mesh device
    keyword : Metal Mesh Device, Bioseparation
  • Flow organic synthesis with polymer monolith
    keyword : Polymer monlith reactor
  • CO2 separation by polymer monolith
    keyword : Polymer Monolith
  • Bioseparation by Polymer Monolith
    keyword : Polymer Monolith
  • Catalysis with Polymer nanogel
    keyword : Polymer, Nanogel, Catalysis
  • The material fabrication with branched polymer interface
    keyword : Interface, Branched Polymer
  • Biomaterials with Glycopolymer
    keyword : Glycopolymer、Biopolymer, Infection Disease, Controlled polymerization
Current and Past Project
  • Develop 3D printer technology for soft materials and develop 4D materials of soft materials with properties that change in response to stimuli.
Academic Activities
1. Mizuo Maeda, Atsuhi Takahara, Hiromi Kitano, Tetsuji Yamaoka, Yoshiko Miura, Molecular Soft-Interface Science: Principles, Molecular Design, Characterization and Application, Springer, 2019.05.
1. Yoshiko Miura, Controlled Polymerization for the development of bioconjugate polymers and materials, Journal of Materials Chemistry B, 10.1039/C9TB02418B, 2020, 8, 2010-2019, 2020.01, Controlled polymerization through living radical polymerization is widely studied. Controlled polymerization enables synthetic polymers with precise structures, which have the potential for excellent bio-functional materials. This review summarizes the applications of controlled polymers, especially those via living radical polymerization, to biofunctional materials and conjugation with biomolecules. In the case of polymer ligands like glycopolymers, the polymers control the interactions with proteins and cells based on the precise polymer structures. Living radical polymerization enables the conjugation of polymers to proteins, antibodies, nucleic acids and cells. Those polymer conjugations are a sophisticated method to modify bio-organisms. The polymer conjugations expand the potential of biofunctional materials and are useful for understanding biology..
1. Yoshiko Miura, Masanori Nagao, Hikaru Matsumoto, De Novo designed glycopolymer by the precise polymer synthesis, Chemistry Letters,, 2023.12, Synthetic polymers with molecular recognition ability were investigated. Well-defined chemical structures of biopolymers were mimicked by controlled polymerization. In particular, the preparation and molecular recognition of de novo designed glycopolymers were studied. Glycopolymers such as block polymers, star polymers, and microgels were prepared. The designed glycopolymers showed strong molecular recognition against target proteins and viruses..
2. Masanori Nagao, Aya Horie, Hikaru Matsumoto, Yu Hoshino, Yoshiko Miura, Continuous-Flow PET-RAFT Polymerization in a Packed-Bed Reactor with Porphyrin-Immobilized Silica Particles, Industrial & Engineering Chemistry Research,, 2023.12, Photoinduced electron/energy transfer-reversible addition–fragmentation chain transfer (PET-RAFT) polymerization enables the production of well-controlled synthetic polymers under mild conditions by using visible-light energy. Although flow reactors are suitable for photoreactions in terms of light penetration, contamination of photocatalysts occurs in a homogeneous system and necessitates product purification. Immobilizing the photocatalysts onto supports can eliminate the need for this purification step and enhance the potential for industrial applications of PET-RAFT polymerization. Herein, we report the development of a packed-bed flow photoreactor wherein a photocatalyst for PET-RAFT polymerization, zinc tetraphenylporphyrin, was immobilized onto packed silica particles. Continuous PET-RAFT polymerization of a model monomer (N,N-dimethylacrylamide) was achieved without leakage of the porphyrin molecules. The effects of various reaction conditions, such as the residence time, catalyst density, and target molecular weight, on the polymerization reaction were evaluated. This work contributes to the realization of a facile and practical manufacturing process for highly valuable synthetic polymers..
3. Hiroyuki Koide, Chiaki Kiyokawa, Anna Okishima, Kaito Saito, Keiichi Yoshimatsu, Tatsuya Fukuta, Yu Hoshino, Tomohiro Asai, Yuri Nishimura, Yoshiko Miura, Naoto Oku, Kenneth J. Shea, Design of an Anti-HMGB1 Synthetic Antibody for In Vivo Ischemic/Reperfusion Injury Therapy, Journal of the American Chemical Society,, 2023.10, High-mobility group box 1 (HMGB1) is a multifunctional protein. Upon injury or infection, HMGB1 is passively released from necrotic and activated dendritic cells and macrophages, where it functions as a cytokine, acting as a ligand for RAGE, a major receptor of innate immunity stimulating inflammation responses including the pathogenesis of cerebral ischemia/reperfusion (I/R) injury. Blocking the HMGB1/RAGE axis offers a therapeutic approach to treating these inflammatory conditions. Here, we describe a synthetic antibody (SA), a copolymer nanoparticle (NP) that binds HMGB1. A lightly cross-linked N-isopropylacrylamide (NIPAm) hydrogel copolymer with nanomolar affinity for HMGB1 was selected from a small library containing trisulfated 3,4,6S-GlcNAc and hydrophobic N-tert-butylacrylamide (TBAm) monomers. Competition binding experiments with heparin established that the dominant interaction between SA and HMGB1 occurs at the heparin-binding domain. In vitro studies established that anti-HMGB1-SA inhibits HMGB1-dependent ICAM-1 expression and ERK phosphorylation of HUVECs, confirming that SA binding to HMGB1 inhibits the proteins’ interaction with the RAGE receptor. Using temporary middle cerebral artery occlusion (t-MCAO) model rats, anti-HMGB1-SA was found to accumulate in the ischemic brain by crossing the blood–brain barrier. Significantly, administration of anti-HMGB1-SA to t-MCAO rats dramatically reduced brain damage caused by cerebral ischemia/reperfusion. These results establish that a statistical copolymer, selected from a small library of candidates synthesized using an “informed” selection of functional monomers, can yield a functional synthetic antibody. The knowledge gained from these experiments can facilitate the discovery, design, and development of a new category of drug..
4. Masanori Nagao, Hikaru Matsumoto, Yoshiko Miura , Design of Glycopolymers for Controlling the Interactions with Lectins, Chemistry – An Asian Journal, 10.1002/asia.202300643, 10.1002/asia.202300643, 2023.08, Carbohydrates are involved in life activities through the interactions with their corresponding proteins (lectins). Pathogen infection and the regulation of cell activity are controlled by the binding between lectins and glycoconjugates on cell surfaces. A deeper understanding of the interactions of glycoconjugates has led to the development of therapeutic and preventive methods for infectious diseases. Glycopolymer is one of the classes of the materials present multiple carbohydrates. The properties of glycopolymers can be tuned through the molecular design of the polymer structures. This review focuses on research over the past decade on the design of glycopolymers with the aim of developing inhibitors against pathogens and manipulator of cellular functions..
5. Hikaru Matsumoto, Yu Hoshino, Tomohiro Iwai, Masaya Sawamura, Yoshiko Miura, Sheltering Mono‐P‐Ligated Metal Complexes in Porous Polystyrene Monolith: Effect of Aryl Pendant Stabilizers on Catalytic Durability, Chemistry A European Journal, 10.1002/chem.202301847, 29, 55, e202301847, Chemistry A European Journal, 2023, 29, 55, e202301847, 2023.07, Metal centers that can generate coordinatively unsaturated metals in accessible and stable states have been developed using synthetic polymers with sophisticated ligand and scaffold designs, which required synthetic efforts. Herein, we report a simple and direct strategy for producing polymer-supported phosphine-metal complexes, which stabilizes mono-P-ligated metals by modulating the electronic properties of the aryl pendant groups in the polymer platform. A three-fold vinylated PPh3 was copolymerized with a styrene derivative and a cross-linker to produce a porous polystyrene-phosphine hybrid monolith. Based on the Hammett substituent constants, the electronic properties of styrene derivatives were modulated and incorporated into the polystyrene backbone to stabilize the mono-P-ligated Pd complex via Pd-arene interactions. Through NMR, TEM, and comparative catalytic studies, the polystyrene-phosphine hybrid, which induces selective mono-P-ligation and moderate Pd-arene interactions, demonstrated high catalytic durability for the cross-coupling of chloroarenes under continuous-flow conditions..
6. Yusuke Saito, Ryutaro Honda, Sotaro Akashi, Hinata Takimoto, Masanori Nagao, Yoshiko Miura, Yu Hoshino, Polymer Nanoparticles with Uniform Monomer Sequences for Sequence Specific Peptide Recognition, Angewandte Chemie, 10.1002/ange.202206456, 2022.05, Synthetic polymer nanoparticles (NPs) that recognize and neutralize target biomacromolecules are of considerable interest as “plastic antibodies”, synthetic mimics of antibodies. However, monomer sequences in the synthetic NPs are heterogeneous. The heterogeneity limits the target specificity and safety of the NPs. Herein, we report the synthesis of NPs with uniform monomer sequences for recognition and neutralization of target peptides. A multifunctional oligomer with a precise monomer sequence that recognizes the target peptide was prepared via cycles of reversible addition–fragmentation chain transfer (RAFT) polymerization and flash chromatography. The oligomer or blend of oligomers was used as a chain transfer agent and introduced into poly(N-isopropyl acrylamide) hydrogel NPs by radical polymerization. Evaluation of the interaction with the peptides revealed that multiple oligomers in NPs cooperatively recognized the sequence of the target peptide and neutralized its toxicity. Effect of sequence, combination, density and molecular weight distribution of precision oligomers on the affinity to the peptides was also investigated..
7. M Nagao, A Yamaguchi, T Matsubara, Y Hoshino, T Sato, Y Miura, De Novo Design of Star-Shaped Glycoligands with Synthetic Polymer Structurestoward an Influenza Hemagglutinin Inhibitor, Biomacromolecules, 10.1021/acs.biomac.1c01483, 23, 3, 1232-1241, Biomacromolecules 2022, 23, 3, 1232–1241, 2021.12, Synthetic polymers with well-defined structures allow the development of nanomaterials with additional functions beyond biopolymers. Herein, we demonstrate de novo design of star-shaped glycoligands to interact with hemagglutinin (HA) using well-defined synthetic polymers with the aim of developing an effective inhibitor for the influenza virus. Prior to the synthesis, the length of the star polymer chains was predicted using the Gaussian model of synthetic polymers, and the degree of polymerization required to achieve multivalent binding to three carbohydrate recognition domains (CRDs) of HA was estimated. The star polymer with the predicted degree of polymerization was synthesized by reversible addition–fragmentation chain transfer (RAFT) polymerization, and 6′-sialyllactose was conjugated as the glycoepitope for HA. The designed glycoligand exhibited the strongest interaction with HA as a result of multivalent binding. This finding demonstrated that the biological function of the synthetic polymer could be controlled by precisely defining the polymer structures..
8. T Oh , T Uemura, M Nagao, Y Hoshino, Y Miura, A QCM study of strong carbohydrate–carbohydrate interactions of glycopolymerscarrying mannosides on substrates, Journal of Materials Chemistry B, 10.1039/D1TB02344F, 10, 2597-2601, J. Mater. Chem. B, 2022, 10, 2597-2601 10.1039/D1TB02344F (Paper), 2021.12, Carbohydrates on cell surfaces are known to interact not only with lectins but also with other carbohydrates; the latter process is known as a carbohydrate–carbohydrate interaction. Such interactions are observed in complex oligosaccharides. It would be surprising if these interactions were observed in simple monosaccharides of mannose. In this study, the interaction between glycopolymers carrying monosaccharides of mannose was quantitatively investigated by quartz crystal microbalance measurements. We measured the interactions with glycopolymers carrying mannose, galactose and glucose. Surprisingly, the interaction between the glycopolymers and mannose was much stronger than that between other saccharides..
9. T Ishida , M Nagao, T Oh, T Mori, Y Hoshino, Y Miura, Synthesis of Glycopolymers Carrying 3’-Sialyllactose for Suppressing InflammatoryReaction via Siglec-E, Chemistry Letters, 51, 3, 308-311, Chem. Lett. 2022, 51, 308–311 | doi:10.1246/cl.210740, 2021.12, [URL], One of the new strategies to treat autoimmune diseases is to target Siglec, a membrane protein receptor with the ability to suppress immune responses. Herein, we synthesized glycopolymers carrying 3′-sialyllactose in various glycounit densities. RAW 264.7 macrophages transfected to express secreted alkaline phosphatase (SEAP) were used to evaluate the immunosuppression ability of the glycopolymers. The inhibition of the signal transmission was dependent on the glycounit densities of the glycopolymers, and was maximized at the moderate density (70%)..
10. S Nonaka, H Matsumoto, M Nagao, Y Hoshino, Y Miura, Investigation of the effect of microflow reactor diameter on condensationreactions in l-proline-immobilized polymer monoliths, Reaction Chemistry & Engineering, 10.1039/D1RE00386K, 7, 1, 55-60, 10.1039/D1RE00386K (Communication) React. Chem. Eng., 2022, 7, 55-60, 2021.11, The effect of monolith structure and monolith reactor inner diameter on the residence time distribution (RTD), and the relationship between RTD and the catalytic efficiency of the asymmetric aldol addition reaction between p-nitrobenzaldehyde and cyclohexanone were examined. A monolith column containing L-proline as a catalyst was prepared using poly(ethylene glycol) (PEG) as the porogen. The monolith column prepared with PEG with a molecular weight of 6000 Da displayed a narrow pore size distribution and showed a controlled RTD. The performance of monolith reactors with different inner diameters (micro- and millireactors, 0.53 and 4.00 mm) was compared: the microreactor displayed a narrower RTD and a higher turnover number for the asymmetric aldol addition reaction than the millireactor. The different linear flow velocities in the microreactor did not affect the catalytic reaction efficiency and enantioselectivity, demonstrating that the RTDs can be controlled regardless of the flow velocity..
11. Benshuai Guo, Yoshiko Miura, Yu Hoshino, Rational Design of Thermocells Driven by the Volume Phase Transition of Hydrogel Nanoparticles, ACS Applied Materials & Interfaces, 10.1021/acsami.1c07266, 13, 27, 32184-32192, ACS Appl. Mater. Interfaces 2021, 13, 27, 32184–32192, 2021.10, Thermocells are thermoelectrochemical conversion systems for harvesting low-temperature thermal energy. Liquid-state thermocells are particularly desirable because of low cost and their high conversion efficiency at temperatures around physiological temperature, and they have, thus, been extensively studied. However, the performance of the thermocells has to be improved to utilize them as energy chargers and/or batteries. Recently, we reported that a liquid-state thermocell driven by the volume phase transition of hydrogel nanoparticles showed highly efficient thermoelectric conversion with Seebeck coefficient (Se) of −6.7 mV K–1. Here, we report the design rationale of the thermocells driven by the phase transition. A high Se of −9.5 mV K–1 was achieved at temperature between 36 and 40 °C by optimizing choice and amount of redox chemical species. The figure of merit (ZT) of the thermocell was improved by selecting appropriate electrolyte salt to increase the ionic conductivity and prevent the precipitation of nanoparticles. Furthermore, screening of nanoparticles revealed the high correlation between Se and the pH shift generated as a result of phase transition of the nanoparticles. After optimization, the maximum ZT of 8.0 × 10–2 was achieved at a temperature between 20 and 70 °C..
12. Masanori Nagao, Takeshi Uemura, Tasuku Horiuchi, Yu Hoshino, , Screening of a glycopolymer library for GM1 mimetics synthesized by the “carbohydrate module method”, Chemical Communications, 10.1039/D1CC04394C, 57, 10871-10874, Chem. Commun., 2021, 57, 10871-10874, 2021.09, The “carbohydrate module method” is a promising approach for oligosaccharide mimetics using polymeric materials. However, it is difficult to predict the optimal structure for a particular oligosaccharide mimetic, and an efficient strategy for the synthesis and evaluation of glycopolymers is desirable. In this study, a screening of glycopolymers for the “carbohydrate module method” by a combination of photoinduced electron/energy transfer-reversible addition–fragmentation chain-transfer (PET-RAFT) polymerization and surface plasmon resonance imaging (SPRI) is demonstrated. The facile and fast screening of synthetic glycomimetics was achieved, and the glycopolymer with the optimal structure as a GM1 mimetic strongly interacted with the cholera toxin B subunit..
13. Masanori Nagao, Masaya Kichize, Yu Hoshino, Yoshiko Miura, Influence of Monomer Structures for Polymeric Multivalent Ligands: Consideration of the Molecular Mobility of Glycopolymers, Biomacromolecules, 10.1021/acs.biomac.1c00553, 22, 7, 3119-3127, Biomacromolecules 2021, 22, 7, 3119–3127, 2021.06, Molecular mobility is important for interactions of biofunctional polymers with target molecules. Monomer structures for synthetic biofunctional polymers are usually selected based on their compatibility with polymerization systems, whereas the influence of monomer structures on the interaction with target molecules is hardly considered. In this report, we evaluate the correlation between the monomer structures of glycopolymers and their interactions with concanavalin A (ConA) with respect to the molecular mobility. Two types of glycopolymers bearing mannose are synthesized with acrylamide or acrylate monomers. Despite the similar structures, except for amide or ester bonds in the side chains, the acrylate-type glycopolymers exhibit stronger interaction with ConA both in the isothermal titration calorimetry measurement and in a hemagglutination inhibition assay. Characterization of the acrylate-type glycopolymers suggests that the higher binding constant arises from the higher molecular mobility of mannose units, which results from the rotational freedom of ester bonds in their side chains..
14. Yu Hoshino, Tomohiro Gyobu, Kazushi Imamura, Akira Hamasaki, Ryutaro Honda, Ryoga Horii, Chie Yamashita, Yuki Terayama, Takeshi Watanabe, Shoma Aki, Yida Liu, Junko Matsuda, Yoshiko Miura, Ikuo Taniguchi, Assembly of Defect-Free Microgel Nanomembranes for CO2 Separation, ACS Appl. Mater. Interfaces , 10.1021/acsami.1c06447, 13, 25, 30030-30038, ACS Appl. Mater. Interfaces 2021, 13, 25, 30030–30038, 2021.06, The development of robust and thin CO2 separation membranes that allow fast and selective permeation of CO2 will be crucial for rebalancing the global carbon cycle. Hydrogels are attractive membrane materials because of their tunable chemical properties and exceptionally high diffusion coefficients for solutes. However, their fragility prevents the fabrication of thin defect-free membranes suitable for gas separation. Here, we report the assembly of defect-free hydrogel nanomembranes for CO2 separation. Such membranes can be prepared by coating an aqueous suspension of colloidal hydrogel microparticles (microgels) onto a flat, rough, or micropatterned porous support as long as the pores are hydrophilic and the pore size is smaller than the diameter of the microgels. The deformability of the microgel particles enables the autonomous assembly of defect-free 30–50 nm-thick membrane layers from deformed ∼15 nm-thick discoidal particles. Microscopic analysis established that the penetration of water into the pores driven by capillary force assists the assembly of a defect-free dense hydrogel layer on the pores. Although the dried films did not show significant CO2 permeance even in the presence of amine groups, the permeance dramatically increased when the membranes are adequately hydrated to form a hydrogel. This result indicated the importance of free water in the membranes to achieve fast diffusion of bicarbonate ions. The hydrogel nanomembranes consisting of amine-containing microgel particles show selective CO2 permeation (850 GPU, αCO2/N2 = 25) against post-combustion gases. Acid-containing microgel membranes doped with amines show highly selective CO2 permeation against post-combustion gases (1010 GPU, αCO2/N2 = 216) and direct air capture (1270 GPU, αCO2/N2 = 2380). The membrane formation mechanism reported in this paper will provide insights into the self-assembly of soft matters. Furthermore, the versatile strategy of fabricating hydrogel nanomembranes by the autonomous assembly of deformable microgels will enable the large-scale manufacturing of high-performance separation membranes, allowing low-cost carbon capture from post-combustion gases and atmospheric air..
15. 2020,8, 10101-10107.
16. Yoshiko Miura, Controlled polymerization for the development of bioconjugate polymers and material, Journal of Materials Chemistry B, 10.1039/c9tb02418b, 8, 10, 2010-2019, 2020.03, Controlled polymerization through living radical polymerization is widely studied. Controlled polymerization enables synthetic polymers with precise structures, which have the potential for excellent bio-functional materials. This review summarizes the applications of controlled polymers, especially those via living radical polymerization, to biofunctional materials and conjugation with biomolecules. In the case of polymer ligands like glycopolymers, the polymers control the interactions with proteins and cells based on the precise polymer structures. Living radical polymerization enables the conjugation of polymers to proteins, antibodies, nucleic acids and cells. Those polymer conjugations are a sophisticated method to modify bio-organisms. The polymer conjugations expand the potential of biofunctional materials and are useful for understanding biology..
17. Yu Hoshino, Shohei Taniguchi, Hinata Takimoto, Sotaro Akashi, Sho Katakami, Yusuke Yonamine, Yoshiko Miura, Homogeneous Oligomeric Ligands Prepared via Radical Polymerization that Recognize and Neutralize a Target Peptide, Angewandte Chemie - International Edition, 10.1002/anie.201910558, 59, 2, 679-683, 2020, 132,2,689-693., 2020.01, Abiotic ligands that bind to specific biomolecules have attracted attention as substitutes for biomolecular ligands, such as antibodies and aptamers. Radical polymerization enables the production of robust polymeric ligands from inexpensive functional monomers. However, little has been reported about the production of monodispersed polymeric ligands. Herein, we present homogeneous ligands prepared via radical polymerization that recognize epitope sequences on a target peptide and neutralize the toxicity of the peptide. Taking advantage of controlled radical polymerization and separation, a library of multifunctional oligomers with discrete numbers of functional groups was prepared. Affinity screening revealed that the sequence specificity of the oligomer ligands strongly depended on the number of functional groups. The process reported here will become a general step for the development of abiotic ligands that recognize specific peptide sequences..
18. Yuri Kimoto, Yuhei Terada, Yu Hoshino, Yoshiko Miura, Screening of a Glycopolymer Library of GM1 Mimics Containing Hydrophobic Units Using Surface Plasmon Resonance Imaging, ACS Omega, 10.1021/acsomega.9b02877, 4, 24, 20690-20696, 2019, 4, 24, 20690-20696., 2019.11, Effective screening methods for the development of glycopolymers as molecular recognition materials are desirable for the discovery of novel biofunctional materials. A glycopolymer library was prepared to obtain guidelines for the design of glycopolymers for the recognition of cholera toxin B subunits (CTB). Glycopolymers with varying ratios of hydrophobic and sugar units were synthesized by reversible addition fragmentation chain transfer polymerization. N-tert-Butylacrylamide, N-phenylacrylamide, and N-cyclohexylacrylamide as hydrophobic units were copolymerized in the polymer backbone, and galactose, which contributes to CTB recognition, was introduced into the side chains by "post-click" chemistry. The thiol-terminated glycopolymers were immobilized on a gold surface. The polymer immobilization substrate was analyzed in terms of interaction with galactose recognition proteins (CTB, peanut agglutinin, and Ricinus communis agglutinin I) using surface plasmon resonance imaging. The polymers with high ratios of sugar and hydrophobic units had the strongest interactions with the CTB, which was different from the trend with peanut agglutinin and Ricinus communis agglutinin I. The binding constant of the CTB with the glycopolymer with hydrophobic units was 4.1 × 106 M-1, which was approximately eight times larger than that of the polymer without hydrophobic units. A correlation was observed between the log P value and the binding constant, indicating that the hydrophobic interaction played an important role in binding. New guidelines for the design of recognition materials were obtained by our screening method..
19. Takahiro Oh, Kazuki Jono, Yuri Kimoto, Yu Hoshino, Yoshiko Miura, Preparation of multifunctional glycopolymers using double orthogonal reactions and the effect of electrostatic groups on the glycopolymer–lectin interaction, Polymer Journal, 10.1038/s41428-019-0244-x, 51, 12, 1299-1308, 2019, 51,12,1299-1308, 2019.08, We investigated synthetic biomacromolecules to control molecular interactions. Multifunctional glycopolymers for molecular recognition were prepared via living radical polymerization and post-click chemistry with orthogonal Huisgen and thiol-epoxy reactions. The synthesis of the polymer backbone and the subsequent side-chain introduction successfully proceeded in high yield. The multifunctional glycopolymers had a tri-block structure: the first and third blocks contained mannose, and the second block contained either a positively or negatively charged group or a neutral hydrophilic group. The molecular recognition of the glycopolymers toward lectin was evaluated via fluorescence quenching measurements. Because of the electrostatic interaction, the binding constant varied in the following order: positively charged glycopolymer (PT110) > negatively charged glycopolymer (NT110). The effect of the electrostatic interactions was modest compared with the effect of the carbohydrate–lectin binding. These results suggested that the carbohydrate–lectin interaction was an important factor in the molecular recognition of glycopolymers. This study provides guidelines for the preparation of multifunctional polymers, such as biomaterials..
20. Kenneth J. Shea, @Hiroyuki Koide, Yoshiko Miura, Yu Hoshino, Yuri Nishimura, Naoto Oku, Abiotic Anti-VEGF Nanoparticle, United States Patent Application, 2019.07, The present invention relates generally to compositions and methods comprising abiotic, synthetic polymers with affinity and specificity to proteins. The synthetic polymers are an improvement over biological agents by providing a simpler, less expensive, and customizable platform for binding to proteins. In one embodiment, the compositions and methods relate to synthetic polymers with affinity and specificity to vascular endothelial growth factor (VEGF). In one embodiment, the compositions are useful for treating diseases and disorders related to the overexpression of VEGF. In one embodiment, the compositions are useful for treating cancer. In one embodiment, the compositions are useful for detecting VEGF levels from biological samples. In one embodiment, the compositions are useful for detecting overexpression of VEGF from biological samples. In one embodiment, the compositions are used to diagnose cancer..
21. Nagao,M., Matsubara, T., Hoshino, Y., Sato, T., Miura, Y., Synthesis of Various Glycopolymers Bearing Sialyllactose and the Effectof Their Molecular Mobility on Interaction with the Influenza Virus, Biomacromolecules, doi/10.1021/acs.biomac.9b00515, 20, 7, 2763-2769, 2019, 20, 7, 2763-2769, 2019.06.
22. Masanori Nagao, Teruhiko Matsubara, Yu Hoshino, Toshinori Sato, and Yoshiko Miura, Topological Design of Star Glycopolymers for Controlling the Interaction with the Influenza Virus, Bioconjugate Chemistry, 10.1021/acs.bioconjchem.9b00134, 30, 1192-1198, Bioconjugate Chemistry 2019,30,1192-1198, 2019.04, The precise design of synthetic polymer ligands using controlled polymerization techniques provides an advantage for the field of nanoscience. We report the topological design of glyco-ligands based on synthetic polymers for targeting hemagglutinin (HA, lectin on the influenza virus). To achieve precise arrangement of the glycounits toward the sugar-binding pockets of HA, triarm star glycopolymers were synthesized. The interaction of the star glycopolymers with HA was found to depend on the length of the polymer arms and was maximized when the hydrodynamic diameter of the star glycopolymer was comparable to the distance between the sugar-binding pockets of HA. Following the formula of multivalent interaction, the number of binding sites in the interaction of the glycopolymers with HA was estimated as 1.8–2.7. Considering one HA molecule has three sugar-binding pockets, these values were reasonable. The binding mode of synthetic glycopolymer–ligands toward lectins could be tuned using controlled radical polymerization techniques..
23. Nagao Masanori, Hoshino Yu, Miura Yoshiko, Quantitative preparation of multiblock glycopolymers bearing glycounits at the terminal segments by aqueous reversible addition–fragmentation chain transfer polymerization of acrylamide monomer, Journal of Polymer Science, Part A: Polymer Chemistry, 10.1002/pola.29344, 57, 857-861, 2019,57,857-861, 2019.03.
24. Terada, Y.; Hoshino, Y.; Miura, Y., “Glycopolymers mimicking GM1 gangliosides: Cooperativity of galactose and neuraminic acid for cholera toxin recognition, Chemistry–An Asian Journal, 10.1002/asia.201900053, 14, 1021-1027, Chemistry–An Asian Journal 2019,14,1021-1027. (DOI:, 2019.03.
25. Yu Hoshino, Kazushi Imamura, Tomohiro Gyobu, Ikuo Taniguchi, Akira Hamasaki, Chie Yamashita, Takeshi Watanabe, Yoshiko Miura, , Monolayer, composite, gas separation material, filter, gas separation device and method for manufacturing composite, United States Patent Application, 2019.03, A monolayer membrane containing gelling polymer particles having at least one of a basic functional group and an acidic functional group, and having a thickness of less than 5 μm. A composite having a porous carrier and gelling polymer particles having at least any one of a basic functional group and an acidic functional group and filling up the surface pores of the porous carrier. The invention can provide a novel material capable of efficiently separating an acid gas from a mixed gas..
26. Takahiro Oh, Masanori Nagao, Yu Hoshino , and Yoshiko Miura, Self-Assembly of a Double Hydrophilic Block Glycopolymer and the Investigation of Its Mechanism, Langmuir, 10.1021/acs.langmuir.8b01527, 2018.07, [URL].
27. Hikaru Matsumoto, Takanori Akiyoshi, Yu Hoshino, and Yoshiko Miura, Size-tuned hydrogel network of palladium-confining polymer particles: a highly active and durable catalyst for Suzuki coupling reactions in water and ambient temperature, Polymer Jornal, 10.1038/ s41428-018-0102-2, 2018.07.
28. Xinnan Cui, Tatsuya Murakami, Yukihiko Tamura, Kazuhiro Aoki, Yu Hoshino, and Yoshiko Miura, Bacterial Inhibition and Osteoblast Adhesion on Ti Alloy Surfaces Modified by Poly(PEGMA-r-Phosmer) Coating, ACS Appl. Mater. Interfaces, 10.1021/acsami.8b07757, 10, 28, 23674-23681, 2018, 10 (28), 23674–23681, 2018.06, [URL], We have synthesized and immobilized PEGMA500-Phosmer to Ti6Al4V surfaces by a simple procedure to reduce bacteria-associated infection without degrading the cell response. Adhered bacteria coverage was lessened to 1% on polymer-coated surfaces when exposed to Escherichia coli, Staphylococcus epidermidis, and Streptococcus mutans. Moreover, PEGMA500-Phosmer and homoPhosmer coatings presented better responses to MC3T3-E1 preosteoblast cells when compared with the results for PEGMA2000-Phosmer-coated and raw Ti alloy surfaces. The behavior of balancing bacterial inhibition and cell attraction of the PEGMA500-Phosmer coating was explained by the grafted phosphate groups, with an appropriate PEG brush length facilitating greater levels of calcium deposition and further fibronectin adsorption when compared with that of the raw Ti alloy surface..
29. Hiroyuki Koide, Keiichi Yoshimatsu, Yu Hoshino, Shih-Hui Lee, Saki Arizumi, Yudai Narita, Yusuke Yonamine, Adam C. Weisman, Yuri Nishimura, Naogo Oku, Yoshiko Miura, Kenneth J Shea, A polymer nanoparticle with engineered affinity for a vascular endothelial growth factor (VEGF165), Nature Chemistry, 10.1038/nchem.2749, 9, 715-722, 9, pages 715–722 (2017), 2017.03, [URL], Protein affinity reagents are widely used in basic research, diagnostics and separations and for clinical applications, the most common of which are antibodies. However, they often suffer from high cost, and difficulties in their development, production and storage. Here we show that a synthetic polymer nanoparticle (NP) can be engineered to have many of the functions of a protein affinity reagent. Polymer NPs with nM affinity to a key vascular endothelial growth factor (VEGF165) inhibit binding of the signalling protein to its receptor VEGFR-2, preventing receptor phosphorylation and downstream VEGF165-dependent endothelial cell migration and invasion into the extracellular matrix. In addition, the NPs inhibit VEGF-mediated new blood vessel formation in Matrigel plugs in vivo. Importantly, the non-toxic NPs were not found to exhibit off-target activity. These results support the assertion that synthetic polymers offer a new paradigm in the search for abiotic protein affinity reagents by providing many of the functions of their protein counterparts..
30. Masanori Nagao, Yuuki Kurebayashi, Hirokazu Seto, Tadanobu Takahashi, Takashi Suzuki, Yu Hoshino, Yoshiko Miura, Polyacrylamide backbones for polyvalent bioconjugates using “post-click” chemistry”, Polymer Chemistry, 2016.07.
31. Yoshiko Miura, Yu Hoshino, Hirokazu Seto, Glycopolymer Nanobiotechnology, Chemical Reviews, 10.1021/acs.chemrev.5b00247, 116, 1673-1692, 2016.02, Previous studies have clearly shown the importance of the multivalent effect in saccharide–protein interactions. To investigate the multivalent effect, the use of multivalent compounds or “glycoclusters” is indispensable, and many groups have reported syntheses of glycocluster compounds. Examples of glycoclusters include liposomes with glycolipids, glycocalixarenes, glycocyclodextrins, glycopeptides, and glycopolymers. Among the various synthetic glycoclusters, glycopolymers have been the subject of much attention . In this review, we define glycopolymers as polymers carrying pendant saccharides. Since glycopolymers have larger valencies than other multivalent compounds, they show the largest amplification effects in molecular recognition. Glycopolymers are able to be prepared as nanomaterials by controlled polymerization. In this section of the review, we discuss glycopolymers and their application for biotechnology..
32. Xinnan Cui, Hirokazu Seto, Tatsuya Murakami, Yu Hoshino, Yoshiko Miura, Inhibition of Bacterial Adhesion on Hydroxyapatite Model Teeth by Surface Modification with PEGMA-Phosmer Copolymers, ACS Biomater. Sci. Eng, 10.1021/acsbiomaterials.5b00349, 2, 2, 205-212, 2016.02, Modification of the interface properties on hydroxyapatite and tooth enamel surfaces was investigated to fabricate bacterial resistance in situ. A series of copolymers containing pendants of poly(ethylene glycol) methyl ether methacrylate (PEGMA) and ethylene glycol methacrylate phosphate (Phosmer) were polymerized by conventional free radical polymerization and changing the feed ratio of monomers. The copolymers were immobilized on hydroxyapatite and tooth enamel via the affinity of phosphate groups to hydroxyapatite to form the stable and durable polymer brushes on the surfaces. The amounts of polymer immobilized depended on the phosphate group ratio in the copolymers. Surface modification altered the interfacial properties of hydroxyapatite and inhibited bacterial adhesion. Copolymers containing 40–60% PEGMA segments showed a significant inhibitory effect on bacterial adhesion of S. epidermidis both in the presence and absence of plaque model biomacromolecules..
33. Seto, Hirokazu; Ogata, Yutaro; Murakami, Tatsuya; Hoshino, Yu; Miura, Yoshiko , Selective Protein Separation Using Siliceous Materials with a Trimethoxysilane-Containing Glycopolymer, ACS Applied Materials & Interfaces, 10.1021/am2014713, 4, 1, 411-417, 2012, 4(1), 411-417, 2012.01, A copolymer with α-d-mannose (Man) and trimethoxysilane (TMS) units was synthesized for immobilization on siliceous matrices such as a sensor cell and membrane. Immobilization of the trimethoxysilane-containing copolymer on the matrices was readily performed by incubation at high heat. The recognition of lectin by poly(Man-r-TMS) was evaluated by measurement with a quartz crystal microbalance (QCM) and adsorption on an affinity membrane, QCM results showed that the mannose-binding protein, concanavalin A, was specifically bound on a poly(Man-r-TMS)-immobilized cell with a higher binding constant than bovine serum albumin. The amount of concanavalin A adsorbed during permeation through a poly(Man-r-TMS)-immobilized membrane was higher than that through an unmodified membrane. Moreover, the concanavalin A adsorbed onto the poly(Man-r-TMS)-immobilized membrane was recoverable by permeation of a mannose derivative at high concentration..
34. Matsumoto, Erino; Nishizawa, Kazuki; Fukuda, Tomohiro; Takai, Madoka; Miura, Yoshiko, Separation capability of proteins using microfluidic system with dendrimer modified surface , Transactions of the Materials Research Society of Japan, 36, 4, 541-544, 2011、36(4)、541-544, 2011.11.
35. Masaya Wada, Yuta Miyazawa, Yoshiko Miura, A specific Inhibitory effect of multivalent trehalose toward amyloid beta (1-40) aggregation, Polymer Chemistry, accepted, 2011.07.
36. Erino Matsumoto, Tomohiro Fukuda, Yoshiko Miura, Bioinert surface to protein adsorption with higher generation of dendrimer SAMs, Colloids and Surfaces B:Biointerfaces, doi:10.1016/j.colsurfb.2011.01.003, 84, 1, 280-284, 2011.05.
37. Jin Ishii, Masayuki Toyoshima, Miyuki Chikae, Yuzuru Takamura, Yoshiko Miura , Preparation of Glycopolymer-modified Gold Nanoparticles and a New Approach for a Lateral Flow Assay, Bull chem Soc Jpn, doi:10.1246/bcsj.2010030, 84, 5, 466-470, selected paper, 2011.05.
38. Tomohiro Fukuda, Erino Matsumoto, Nobuhiko Yui,and Yoshiko Miura, Peculiar Wettability Based on Orientational Change of Self-assembled Hemispherical PAMAM Dendrimer Layer, Chemistry Letters, doi:10.1246/cl.2010.923, 39, 9, 923, 2010, 39, 923-925, 2010.07, [URL].
39. T. Fukuda, E. Matsumoto, S. Onogi, Y. Miura, Aggregation of Alzheimer Amyloid β Peptide (1−42) on the Multivalent Sulfonated Sugar Interface, Bioconjugate Chemistry, 10.1021/bc100053x, 21, 6, 1079, 2010, 21, 1079-1086, 2010.06, [URL].
40. M. Toyoshima, T. Oura, T. Fukuda, E. Matsumoto, Y. Miura, , Biological specific recognition of glycopolymermodified interfaces by RAFT living radical polymerization, Polymer Journal, doi:10.1038/pj.2009.321, 42, 172, 2010, 42, 172-178, 2010.02, [URL].
41. yoshiko miura, Inhibition of protein amyloidosis by glycomaterials, Trends in Glycoscience and Glycotechnology, doi:10.4052/tigg.21.324, 21, 122, 324-334, 2009.12.
42. Tomohiro Fukuda, Shunsuke Onogi, Yoshiko Miura, Dendritic Sugar-Microarrays by Click Chemistry, Thin Solid Films, 518, 880-888, 2009.11.
43. Koji Funato, Naoto Shirahata, Yoshiko Miura, The monolayer of a-Man via Si-C bond formation and protein recognition, Thin Solid Films, 518, 699, 2009.11.
44. Yoshiko Miura, Kiyofumi Yamamoto, Kikuko Yasuda, Yoshihiro Nishida, Kazukiyo koabayashi, Inhibition of Alzheimer Amyloid Aggregation with Sulfate Glycopolymers, Advances in Science and Technology , 57, 166-169, 2009.08.
45. Masayuki Toyoshima, Yoshiko Miura, Preparation of GLycopolymer-Substituted Gold nanoparticles and Their Molecular Recognition, Journal of Polymer Science PartA: Polymer Chemistry, 47, 1412-1421, 2009.03.
46. Erino Matsumoto, Takanori Yamauchi, Tomohiro Fukuda, Yoshiko Miura, Sugar microarray by click chemistry, Sci. Technol. Adv. Mater. , 10, 034605, 2009.03.
47. Miyuki Chikae, Tomohiro Fukuda, K. Kerman, K. Idegami, Yoshiko Miura, Eiichi Tamiya, Amyloid beta-detection with saccharide immobilized gold nanoparticle on carbon electrode, Bioelectrochemistry, 74, 118-123, 2008.11.
48. Yoshiko Miura, Takahiro Yamauchi, Hajime Sato, Tomohiro Fukuda, The Self-Assembled Monolayer of Saccharide via Click Chemistry: Formation and Protein Recognition, Thin Solid Films, 516, 2443, 2008.09.
49. Yoshiko Miura, Chouga You, Reiko Ohnishi,, Inhibition of Alzheimer amyloid beta aggregation by polyvalent trehalose, Sci. Technol Adv Mat , 9, 24407, 2008.07.
50. Tomohiro Fukuda, Shunsuke Onogi, Yoshiko Miura, Preparation and Properties of Dendritic Sugar Immobilized Surface, Trans. Mat. Res. Soc. Jpn,, 33, 733, 2008.03.
51. Yoshiko Miura, Shunsuke Onogi, Kiyofumi Yamamoto, Synthesis of Glycodendrimer via Click Chemistry and Protein Affinities, Trans. Mat. Res. Soc. Jpn, 33, 729, 2008.03.
52. Yoshiko Miura, Kikuko Yasuda, Kiyofumi Yamamoto, Mihoko Koike, Yoshihiro Nishida, Kazukiyo Kobayashi, Inhibition of Alzhimer Amyloid Aggregation with Sulfated Glycopolymers , Biomacromolecules, 8, 2129, 2007.11.
53. Yoshiko Miura, Daisuke Kouketsu, kazukiyo Kobayashi, Synthesis and Properties of a Well-Defined Glycopolymer via Living radical Polymerization, Polymer Advanced Technology, 18, 647, 2007.07.
54. Hajime Sato, Yoshiko Miura, Nagahiro Saito, Kazukiyo Kobayashi, Osamu Takai, Fibroblastic Microfabrication by Molecular Recognition on Substrate, Surface Science, 601, 3871, 2007.04.
55. Hajime Sato, Yoshiko Miura, Nagahiro Saito, Kazukiyo Kobayashi, Osamu Takai, A Micropatterned Multifunctional Carbohydrate Display by an Orthogonal Self-Assembling Strategy, Biomacromolecules, 8, 753-756, 2007.01.
56. Yoshiko Miura, Akio Sakaki, Masamichi Kamihira, Shinji Iijima, Kazukiyo Kobayashi, A globotriaosylceramide (Gb3Cer) mimic peptide , Biochimica et Biophysica Acta, 1760, 883, 2006.09.
57. Hajime Sato, Yoshiko Miura, Takahiro Yamauchi, Kazukiyo , Carbohydrate Microarray by Click Chemistry, Trans. Mat. Res. Soc. Jpn, 31, 659, 2006.04.
58. Yoshiko Miura, The Development and the Character of Saccharide Biosensors, Trends in Glycoscience and Glycotechnology, , 18, 349, 2006.04.
59. Yoshiko Miura, Chieri Shibata, Kazukiyo Kobayashi, Theremoresponsive Self-Assembly of Short Elastin-Like Peptides , Trans Mat Res Soc Jpn, 31, 549, 2006.04.
60. Yoshiko Miura, Chieri Shibata, Kazukiyo Kobayashi, Theremoresponsive Self-Assembly of Short Elastin-Like Peptides , Trans Mat Res Soc Jpn, 31, 549, 2006.04.
61. Natsuko Wada, Yoshiko Miura, Kazukiyo Koabayashi, Synthesis and Biological Properties of Glycopolymer-Polylactide Conjugate, Trans. Mat. Res. Soc. Jpn, 32, 767, 2005.04.
62. Yoshiko Miura, Natsuko Wada, Yoshihiro Nishida, H. Mori, K. Kobayashi, Chemoenzymatic Synthesis of Glycoconjugate Polymers Starting from Non-reducing Disaccharides, J. Polym. Sci. part A Polym. Chem. 2004, 42, 4598, 42, 4598, 2004.04.
63. Yoshiko Miura, Yuki Sasao, Masamichi Kamihira, Akio Sakaki, Shinji Iijima, Kazukiyo kobayashi, Peptides binding to a Gb3 mimic selected from a phage library, Biochem. Biophys. Acta, 1673, 131, 2004.04.
64. Y. Miura, T. Ikeda, N. Wada, K. kobayashi, Chemoenzymatic Synthesis of Glycoconjugate Polymers: Greening the Synthesis of biomaterials, Green Chemistry, 5, 610, 2003.04.
65. Y. Miura, T. Ikeda, N. Wada, K. Kobayashi, Chemoenzymatic synthesis of a Multivalent Aminoglycoside, Macromol. Biosci, 3, 362, 2003.04.
66. Yoshiko Miura, takayasu ikeda, kazukiyo kobayashi, Chemoenzymatically Synthesized Glycoconjugate Polymers, Biomacromolecules, 10.1021/bm025714b, 4, 2, 410, 2003.02.
67. Yoshiko Miura, Yuuki Sasao, Hirofumi Dohi, Yoshihiro Nishida and Kazukiyo Kobayashi, Self-assembled monolayers of globotriaosylceramide (Gb3) mimics: surface-specific affinity with shiga toxins , doi:10.1016/S0003-2697(02)00318-4, 310, 27, 2002.04.
68. Y. Miura, S. Kimura, S. Kobayashi, Y. Imanishi, J. Umemura, Cation recognition by self-assembled monolayers of oriented helical peptides having a crown ether unit, Biopolymers, 55, 391, 2000.04.
69. Y. Miura, S. Kimura, Y. Imanishi, J. Umemura, Formation of Oriented Helical Peptide Layers on a Gold Surface due to the Self-assembling Properties of Peptides, Langmuir, 14, 6935, 1998.04.
70. Y. Miura, S. Kimura, Y. Imanishi, J. Umemura, Self-Assembly of a-helix peptide/crown ether conjugate upon complexation with ammonium-terminated alkanethiolate, 14, 2761, 1998.04.
1. Yoshiko Miura, Denovo design of glycopolymer for controlled molecular recognition, Pacific Polymer Conference 17 (PPC17), 2022.12.
Membership in Academic Society
  • Science Council of Japan
  • The Japan Society of Vacuum and Surface Science
  • The Japanese Society of Carbohydrate Research
  • The Society of Chemical Engineering, Japan
  • the Materials Research Society of Japan
  • Polymer Society Japan
  • The Chemical Society of Japan
  • American Chemical Society
  • The manuscript title of "Interaction Analyses of Amyloid beta Peptide (1-40) with Glycosaminoglycan Model Polymers" was selected as BCSJ award by Japan Chemical Society. In this manuscript, the author described the inhibition of Alzheimer disease using biomaterials. Especially, the authors synthesized glycominoglycan mimic polymer which inhibit the aggregation of Alzheimer amyloid beta aggregation.
Educational Activities
I am in charge of Organic Material Chemistry (all undergraduate courses), Basic Physical Chemistry I (second-year students), and Reaction Engineering(third-year students), and Bio-resource Materials Engineering (graduate school) In the laboratory, For graduate and undergraduate students, the program provides individualized instruction in experiments, reading and writing papers, and training in logical thinking. In addition to the research aspect, guidance is provided on daily life in cooperation with their families if needed. For undergraduate and graduate students, I promote and provide guidance for presentations at domestic and international academic conferences. The department participates in university-wide and departmental FD meetings every year to improve education and teaching methods. .
Professional and Outreach Activities
Outreach in Elementary School in Itoshima-city (2012), Lecture in Miyazaki-Kita high school (2013), JGFos (2004)
Japan Academy、Committee of Scholar ship、Lecture in Takamatsu -Daiichi High Shcool.