Updated on 2024/10/07

Information

 

写真a

 
FUJIMURA YOSHINORI
 
Organization
Faculty of Agriculture Department of Bioscience and Biotechnology Associate Professor
School of Agriculture Department of Bioresource and Bioenvironment(Concurrent)
Graduate School of Bioresource and Bioenvironmental Sciences Department of Bioscience and Biotechnology(Concurrent)
Title
Associate Professor
Contact information
メールアドレス
Tel
0928024747
Profile
現在、”食品予防医学の包括的理解に向けて、生体ならびに食品中の低分子生理活性化合物のユニークな挙動に着目した高解像度の表現型解析システム(ケミカルセンシング技術)の創出”をめざして、以下に示した研究テーマを推進している。 (1)フードケミカルバイオロジーに基づく食品予防医学研究 食品中に含まれる低分子化合物をプローブとして、その生体内標的分子の機能解析を通じて生命現象の一端を理解し、それを応用しようとする試み”フードケミカルバイオロジー”を推し進めている。具体的に、食事ポリフェノール・緑茶カテキンの生体内標的分子(緑茶カテキン受容体)を介した機能性発現経路(抗ガンおよび抗アレルギー作用)の解明を目指している。このような研究により得られた研究成果は、特定の機能性食品成分を配合した疾病予防食(個人の効き方に対応したテーラーメイド食)創製に寄与するだけでなく、その食品成分の機能を模倣した全く新しいコンセプトに基づいた分子標的創薬への応用展開が期待される。 (2)多元的質量分析による高精度メタボリック・プロファイリングシステムの開発 レドックス関連疾患(がんや糖尿病など)の病理診断やバイオマーカー探索に必要な低分子量代謝物の高精度網羅的解析方法を確立するため、種々の質量分析計を組合せた統合的解析技術(高精度メタボリック・プロファイリングシステム)の開発を行っている。本システム開発は従来にない高感度で病態標的性の高い質量分析の基盤技術を提供するものであり、臨床における低侵襲的解析への応用をめざしている。 (3)ニュートリメタボロミクスを切り拓く高精度質量分析システムの開発 近年、食品(農林水産物)の品質鑑定・予測ならびにそれら要因解析の新たな手法として、食品中の低分子成分(代謝物)を標的とした代謝物プロファイリング法が注目を集めているが、現状では、それらの食品機能性(三次機能:生体調節機能)分野への応用は皆無である。これに対して、我々は高速液体クロマトグラフ質量分析・多変量統計解析に基づく代謝物プロファイリング法が数十種類の茶(Camellia sinensis L.)品種の機能性評価(機能性品種の識別化や機能性予測モデルの構築/生理活性因子や共存成分バランスの解明など)に極めて有効であることを世界に先駆けて報告している。このような知見は、機能性を付与した新たな品種開発や既存品種の有用性発掘に大いに役立つことが期待される。試料中の複数成分間の相関関係(線形・非線形性)を捉える代謝物プロファイリング法(新たな食品機能性評価メタボロミクス:ニュートリメタボロミクス)は、食品機能性の理解に不可欠な成分間/食品間相互作用に関する基礎情報の取得を可能にし、機能性成分を活用した食品開発や食品の食べ合わせに役立つ新たな科学的根拠の創出に寄与すると思われる。

Research Areas

  • Life Science / Food sciences

  • Life Science / Functional biochemistry

  • Life Science / Applied molecular and cellular biology

  • Humanities & Social Sciences / Family and consumer sciences, and culture and living

  • Life Science / Genome biology

  • Nanotechnology/Materials / Chemical biology

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Degree

  • PhD, Agriculture

Research History

  • Kyushu University Faculty of Agriculture Associate Professor

    2018.4 - Present

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  • 九州大学先端融合医療レドックスナビ研究拠点

Education

  • Kyushu University   School of Agriculture

    - 1998.3

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    Country: Japan

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Research Interests・Research Keywords

  • Research theme:Phytochemical

    Keyword:Phytochemical

    Research period: 2024

  • Research theme:Chemical biology

    Keyword:Chemical biology

    Research period: 2024

  • Research theme:アレルギー

    Keyword:アレルギー

    Research period: 2024

  • Research theme:Nutrimetabolomics

    Keyword:Nutrimetabolomics

    Research period: 2024

  • Research theme:Immunobiochemistry

    Keyword:Immunobiochemistry

    Research period: 2024

  • Research theme:Food Chemistry

    Keyword:Food Chemistry

    Research period: 2024

  • Research theme:Animal cell technology

    Keyword:Animal cell technology

    Research period: 2024

  • Research theme:Allergy

    Keyword:Allergy

    Research period: 2024

  • Research theme:Polyphenol

    Keyword:Polyphenol

    Research period: 2024

  • Research theme:食糧化学

    Keyword:食糧化学

    Research period: 2024

  • Research theme:Food Analysis

    Keyword:Food Analysis

    Research period: 2024

  • Research theme:Mass spectrometry

    Keyword:Mass spectrometry

    Research period: 2024

  • Research theme:

    Keyword:

    Research period: 2024

  • Research theme:細胞工学

    Keyword:細胞工学

    Research period: 2024

  • Research theme:Pathological analysis

    Keyword:Pathological analysis

    Research period: 2024

  • Research theme:機能性食品

    Keyword:機能性食品

    Research period: 2024

  • Research theme:Functional Food Pairing

    Keyword:Functional Food Pairing

    Research period: 2024

  • Research theme:機能性RNA

    Keyword:機能性RNA

    Research period: 2024

  • Research theme:抗体

    Keyword:抗体

    Research period: 2024

  • Research theme:抗がん

    Keyword:抗がん

    Research period: 2024

  • Research theme:免疫化学

    Keyword:免疫化学

    Research period: 2024

  • Research theme:メタボロミクス

    Keyword:メタボロミクス

    Research period: 2024

  • Research theme:Metabolic profiling

    Keyword:Metabolic profiling

    Research period: 2024

  • Research theme:microRNA

    Keyword:microRNA

    Research period: 2024

  • Research theme:R&D of high-precision metabolic profiling system by multiple mass spectrometric analysis

    Keyword:Metabolic profiling, Metabolomics, Mass spectrometry, Redox-related diseases, Biomarker, Pathological diagnosis

    Research period: 2008.7

  • Research theme:Chemical biology based food preventive medicine research

    Keyword:Chemical biology, Target molecule, Preventive medicine, Nutrimetabolomics, Green tea catechin

    Research period: 2000.4

Awards

  • 日本農芸化学会2021年度大会トピックス賞

    2021.4   日本農芸化学会   ウーロン茶ポリフェノールの腸管上皮細胞におけるmiRNA発現調節作用とその機能

  • 日本農芸化学会2021年度大会トピックス賞

    2021.4   日本農芸化学会   植物マイクロRNAの肺線維化抑制作用

  • 第9回(2020年度)三島海雲学術賞

    2020.7   三島海雲記念財団  

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    食品ポリフェノールの生体感知機構と機能性増強技術に関する研究

  • 日本農芸化学会2015年度大会トピックス賞

    2015.4  

  • 平成26年度 農芸化学奨励賞

    2014.3   日本農芸化学会  

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    緑茶の機能性を捉える低分子ケミカルセンシングに関する研究

  • 日本農芸化学会2013年度大会トピックス賞

    2013.4   日本農芸化学会   質量分析イメージング法による緑茶カテキンおよびその代謝物の生体組織内分布情報の非標識可視化

  • International Conference on Food Factors for Health Promotion (ICoFF2007) Poster Award

    2007.11   International Conference on Food Factors for Health Promotion (ICoFF2007) Poster Award The 67 kDa laminin receptor mediates anti-allergic effects of (-)-epigallocatechin-3-O-(3-O-methyl) gallate

  • 平成17年度日本農芸化学会西日本支部奨励賞(一般)

    2006.1   日本農芸化学会  

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    機能性食品成分の標的受容体分子制御に基づく抗アレルギーシグナルの解明

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Papers

  • Regulatory effect of Epigallocatechin-3-O-gallate on circular RNA expression in mouse liver: Regulatory effect of EGCG on circRNA. Reviewed International journal

    Ren Yoshitomi, Motofumi Kumazoe, Kwan-Woo Lee, Yuki Marugame, Yoshinori Fujimura, Hirofumi Tachibana

    The Journal of Nutritional Biochemistry   124   109506 - 109506   2024.2   ISSN:0955-2863 eISSN:1873-4847

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    There are few studies on the connection between food components and circular RNA (circRNA), a type of noncoding RNA that is significant for living organisms. (-)-Epigallocatechin-3-O-gallate (EGCG) has been reported to have various biological effects, and elucidation of the molecular mechanism is important for clarifying the functionality of EGCG. In the current study, we looked at how EGCG regulates the expression of circRNA in the liver, which expresses a lot of circRNAs. Mice were given EGCG (10 mg/kg b.w.) orally for one week before circRNA microarray testing was done on their livers. The microarray analysis revealed that mice treated with EGCG had altered expression of 35 circRNAs in their livers. To clarify the function of mmu_circRNA_011775, one of the circRNAs upregulated by EGCG, mouse liver cells after the mmu_circRNA_011775 expression vector was transfected into NMuLi cells, next-generation sequencing (NGS) was used to analyze the gene expression. NGS analysis shows that the expression of the genes responsible for liver fibrosis and inflammation. Gene ontology (GO) analysis showed that mmu_circRNA_011775 changed the meaning of GO terms associated with the cardiovascular system. In the microarray, EGCG altered 35 genes expression. Among them, pre-ribosomal RNA-derived circRNA mmu_circRNA_011775 regulated the expression of various genes related to liver fibrosis and cardiovascular system.

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  • 67-kDa laminin receptor mediates oolonghomobisflavan B-induced cell growth inhibition in melanoma. Reviewed International journal

    Jaehoon Bae, Motofumi Kumazoe, Kwan-Woo Lee, Yoshinori Fujimura, Hirofumi Tachibana

    Phytomedicine   118   154970 - 154970   2023.9   ISSN:0944-7113 eISSN:1618-095X

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    BACKGROUND: Oolonghomobisflavans are unique polyphenols found in oolong teas. Oolonghomobisflavan B (OHBFB), a dimer of (-)-epigallocatechin-3-O-gallate (EGCG), is an active compound found in green tea. PURPOSE: OHBFB has been reported to exert an inhibitory effect on lipase enzyme activity. However, little is known regarding its intercellular signaling induction effect. Further, there are no reports describing the anti-cancer effects of OHBFB. METHODS: The effect of OFBFB on B16 melanoma cells was evaluated by cell counting, and its mechanisms were determined by western blot analysis with or without protein phosphatase 2A (PP2A) inhibitor treatment. Intracellular cyclic adenosine monophosphate (cAMP) levels were evaluated by time-resolved fluorescence resonance energy transfer analysis. Quartz crystal microbalance (QCM) analysis was performed to assess the binding of OHBFB to 67LR. RESULTS: Cell growth assay and western blot analyses showed that OHBFB inhibited melanoma cell growth, followed by myosin phosphatase target subunit 1 (MYPT1) and myosin regulatory light chain (MRLC) dephosphorylation via protein phosphatase 2A (PP2A)-dependent mechanisms. These effects are mediated by intracellular cAMP- and protein kinase A (PKA) A-dependent mechanisms. QCM analysis identified the 67-kDa laminin receptor (67LR) as an OHBFB receptor with a Kd of 3.7 µM. We also demonstrated for the first time that OHBFB intake suppresses tumor growth in vivo. CONCLUSIONS: Taken together, these results indicate that the cAMP/PKA/PP2A/MYPT1/MRLC pathway is a key mediator of melanoma cell growth inhibition following OHBFB binding to 67LR and that OHBFB suppresses tumor growth in vivo.

    DOI: 10.1016/j.phymed.2023.154970

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  • The anti-cancer effect of epigallocatechin-3-O-gallate against multiple myeloma cells is potentiated by 5,7-dimethoxyflavone. Reviewed International journal

    Jaehoon Bae, Motofumi Kumazoe, Su-Jin Park, Yoshinori Fujimura, Hirofumi Tachibana

    FEBS Open Bio   13 ( 11 )   2147 - 2156   2023.9   ISSN:2211-5463

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    (-)-Epigallocatechin-3-O-gallate (EGCG) is one of the major components of green tea polyphenol. Previous studies have shown that EGCG induces cancer-specific cell death in vitro and in vivo without causing severe side effects. However, the anti-cancer effect of EGCG alone is limited. 5,7-dimethoxyflavone (5,7-DMF), one of the principal functional components of black ginger (Kaempferia parviflora), also exerts anti-cancer effects. Here, we show that 5,7-DMF synergistically enhances the anti-cancer effect of EGCG in multiple myeloma cells by potentiating EGCG-induced intracellular cyclic guanosine monophosphate (cGMP) production. Moreover, the combination of EGCG and 5,7-DMF induces apoptotic cell death in multiple myeloma cells, and this is accompanied by activation of the cGMP/acid sphingomyelinase (ASM)/cleaved caspase-3 pathway. In conclusion, we have shown that 5,7-DMF enhances the anti-cancer effect of EGCG by upregulating cGMP in multiple myeloma cells.

    DOI: 10.1002/2211-5463.13708

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  • PPAR/PDK4 pathway is involved in the anticancer effects of cGMP in pancreatic cancer. Reviewed International journal

    Mai Yamashita, Motofumi Kumazoe, Hiroaki Onda, Shun Hiroi, Yu Shimada, Yoshinori Fujimura, Hirofumi Tachibana

    Biochemical and Biophysical Research Communications   672   154 - 160   2023.9   ISSN:0006-291X eISSN:1090-2104

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    Pancreatic ductal adenocarcinoma (PDAC) is a type of cancer with a high mortality rate. Current treatments for PDACs often have side effects, and drug resistance in cancer stem cells (CSCs) would be also a problem. Cyclic guanosine monophosphate (cGMP) suppresses the mitochondrial function of PDACs and inhibits their CSC properties. Metabolic regulation plays a crucial role in the maintenance of CSC phenotype, and we hypothesized that cGMP induction suppresses cancer stem cell properties in the cancer cell through energy-related signaling pathways. We demonstrated that induction of cGMP upregulated the PPARα/PDK4 pathway and suppressed CSC properties in PDAC, and patients with pancreatic cancer with high PDK4 gene expression had a better prognosis than those with low gene expression. Therefore, these mechanisms may provide new therapeutic targets for the eradication of pancreatic CSCs.

    DOI: 10.1016/j.bbrc.2023.06.043

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  • Dextran sulfate sodium-induced mild chronic colitis induced cognitive impairment accompanied by inhibition of neuronal maturation in adolescent mice Reviewed International journal

    #Kwanwoo Lee,@Motofumi Kumazoe,#Yuki Marugame,@Yoshinori Fujimura, and@Hirofumi Tachibana

    Biochem. Biophys. Res. Commun.   669   46 - 53   2023.6

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    DOI: doi:10.1016/j.bbrc.2023.05.112

  • Bioactivity-boosting strategy based on combination of anti-allergic O-methylated catechin with a Citrus flavanone, hesperetin Reviewed

    Yoshinori Fujimura, Takanori Yoshimoto, Konatsu Fujino, Ayaka Nezu, Yuki Marugame, Jaehoon Bae, Motofumi Kumazoe, Hirofumi Tachibana

    JOURNAL OF NATURAL MEDICINES   77 ( 2 )   363 - 369   2023.3   ISSN:1340-3443 eISSN:1861-0293

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:SPRINGER JAPAN KK  

    Many patients with allergies have anxiety about taking anti-allergic medicines due to their side effects and increased medical expenses. Thus, developing functional foods/agricultural products for allergy prevention is strongly desired. In this study, we revealed that a Citrus flavanone, hesperetin, amplified IgE/antigen-mediated degranulation-inhibitory potency of anti-allergic catechin, (-)-epigallocatechin-3-O-(3-O-methyl) gallate (EGCG3 "Me), in the rat basophilic/mast cell line RBL-2H3. Hesperetin also significantly elevated the activation of acid sphingomyelinase (ASM), essential for eliciting anti-allergic effect of EGCG3 "Me through the cell surficial protein, 67-kDa laminin receptor (67LR). Furthermore, oral administration of the highly absorbent hesperidin, alpha-glucosyl hesperidin, also enhanced the inhibitory potency of EGCG3 "Me-rich 'Benifuuki' green tea (Camellia sinensis L.) on passive cutaneous anaphylaxis (PCA) reaction evoked by IgE/antigen in BALB/c mice. These observations indicate that hesperetin amplifies the ability of EGCG3 "Me to inhibit the IgE/antigen-mediated degranulation through activating ASM signaling.

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  • (-)‑Epigallocatechin‑3‑O‑gallate upregulates the expression levels of miR‑6757‑3p, a suppressor of fibrosis‑related gene expression, in extracellular vesicles derived from human umbilical vein endothelial cells. Reviewed International journal

    Motoki Murata, Yuki Marugame, Mai Morozumi, Kyosuke Murata, Motofumi Kumazoe, Yoshinori Fujimura, Hirofumi Tachibana

    Biomedical Reports   18 ( 3 )   19 - 19   2023.3   ISSN:2049-9434 eISSN:2049-9442

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    As pulmonary fibrosis (PF), a severe interstitial pulmonary disease, has such a poor prognosis, the development of prevention and treatment methods is imperative. (-)-Epigallocatechin-3-O-gallate (EGCG), one of the major catechins in green tea, exerts an antifibrotic effect, although its mechanism remains unclear. Recently, it has been reported that microRNAs (miRNAs or miRs) transported by extracellular vesicles (EVs) from vascular endothelial cells (VECs) are involved in PF. In the present study, the effects of EGCG on the expression of miRNAs in EVs derived from human umbilical vein endothelial cells (HUVECs) were assessed and miRNAs with antifibrotic activity were identified. miRNA microarray analysis revealed that EGCG modulated the expression levels of 31 miRNAs (a total of 27 miRNAs were upregulated, and 4 miRNAs were downregulated.) in EVs from HUVECs. Furthermore, TargetScan analysis indicated that miR-6757-3p in particular, which exhibited the highest degree of change, may target transforming growth factor-β (TGF-β) receptor 1 (TGFBR1). To evaluate the effects of miR-6757-3p on TGFBR1 expression, human fetal lung fibroblasts (HFL-1) were transfected with an miR-6757-3p mimic. The results demonstrated that the miR-6757-3p mimic downregulated the expression of TGFBR1 as well the expression levels of fibrosis-related genes including fibronectin and α-smooth muscle actin in TGF-β-treated HFL-1 cells. In summary, EGCG upregulated the expression levels of miR-6757-3p, which may target TGFBR1 and downregulate fibrosis-related genes, in EVs derived from VECs.

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  • Plant miRNA osa-miR172d-5p suppressed lung fibrosis by targeting Tab1 Reviewed International journal

    @Motofumi Kumazoe,@Fumiyo Ogawa,@Ai Hikida,#Yu Shimada,@Ren Yoshitomi,#Ryoya Watanabe,@Yoshinori Fujimura, and@Hirofumi Tachibana

    Sci. Rep.   13   2128   2023.2

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    DOI: Doi: 10.1038/s41598-023-29188-6

  • Quercetin up-regulates the expression of tumor-suppressive miRNAs in human cervical cancer Reviewed International journal

    @Motoki Murata,@Satomi Komatsu,@Emi Miyamoto,@Chihiro Oka,Ichian Lin,@Motofumi Kumazoe,@Shuya Yamashita,@Yoshinori Fujimura, and @Hirofumi Tachibana

    Bioscience of Microbiota, Food and Health   42   87 - 93   2023.1

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  • (-)-Epigallocatechin-3-O-gallate upregulated the expression levels of miR-6757-3p, a suppressor of fibrosis-related gene expression, in extracellular vesicles derived from human umbilical vein endothelial cells Reviewed International journal

    @Motoki Murata,#Yuki Marugame,@Mai Morozumi,@Kyosuke Murata,@Motofumi Kumazoe,@Yoshinori Fujimura, and@Hirofumi Tachibana

    Biomedical Reports   18   19   2023.1

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    DOI: doi: 10.3892/br.2023.1601

  • Quercetin up-regulates the expression of tumor-suppressive microRNAs in human cervical cancer

    MURATA Motoki, KOMATSU Satomi, MIYAMOTO Emi, OKA Chihiro, LIN Ichian, KUMAZOE Motofumi, YAMASHITA Shuya, FUJIMURA Yoshinori, TACHIBANA Hirofumi

    Bioscience of Microbiota, Food and Health   42 ( 1 )   87 - 93   2023   ISSN:2186-6953 eISSN:21863342

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    <p>Quercetin, a flavonol present in many vegetables and fruits, has been identified as a chemoprevention agent in several cancer models. However, the molecular mechanism of quercetin’s anticancer activity is not entirely understood. MicroRNAs (miRNAs), small noncoding RNAs, have been reported to play key roles in various biological processes by regulating their target genes. We hypothesized that quercetin can exert an anticancer effect through the regulation of miRNAs. To test this hypothesis, we investigated the effects of quercetin on the expression of tumor-suppressive miRNAs in cervical cancer. Quercetin up-regulated the in vivo and in vitro expression of tumor-suppressive miRNAs miR-26b, miR-126, and miR-320a. Quercetin suppressed the level of β-catenin, encoded by catenin beta 1 (CTNNB1), by up-regulating miR-320a in HeLa cells. Moreover, quercetin increased the expression of mir-26b, mir-126, and mir-320a precursors in HeLa cells. The results from this study show that quercetin has the potential to prevent cervical cancer by regulating the expression of tumor-suppressive miRNAs.</p>

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  • Phosphodiesterase 5 Inhibitor Potentiates Epigallocatechin 3-O-Gallate-Induced Apoptotic Cell Death via Activation of the cGMP Signaling Pathway in Caco-2 Cells Invited Reviewed International journal

    @Jaehoon Bae,#Kwanwoo Lee,@Ji Sun Park,@Jinseok Jung,@Hirofumi Tachibana,@Yoshinori Fujimura,@Motofumi Kumazoe,@Jae Sung Lim,@Young-Chang Cho,@Seung-Jae Lee and @Su-Jin Park

    Curr. Issues Mol. Biol.   44   6247 - 6256   2022.12

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    DOI: doi: 10.3390/cimb44120426

  • Bioactivity-boosting strategy based on combination of anti-allergic O-methylated catechin with a Citrus flavanone, hesperetin Reviewed International journal

    @Yoshinori Fujimura,@Takanori Yoshimoto,@Konatsu Fujino,@Ayaka Nezu,#Yuki Marugame,@Jaehoon Bae,@Motofumi Kumazoe, and@Hirofumi Tachibana

    J. Nat. Med.   77   363 - 369   2022.12

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    DOI: doi: 10.1007/s11418-022-01668-5

  • Inflammatory markers S100A8/A9 and metabolic alteration for evaluating signs of early phase toxicity of anticancer agent treatment. Reviewed International journal

    Tomomi Morikawa-Ichinose, Yoshinori Fujimura, Motofumi Kumazoe, Hiroaki Onda, Daisuke Miura, Hirofumi Tachibana

    Food and Chemical Toxicology   169   113421 - 113421   2022.11   ISSN:0278-6915 eISSN:1873-6351

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    Anticancer agents can cause various side effects, including tissue damages/inflammatory reactions. Drug-responsive biomarkers are essential for evaluating drug toxicity in disease processes. S100 calcium-binding proteins A8/A9 (S100A8/A9) are highly expressed in neutrophils and monocytes/macrophages accumulated at inflammatory sites and are known to be related to tissue damage/inflammation; however, their response to drug toxicity has not been reported. Herein, we investigated the effects of anticancer agents (doxorubicin, cisplatin, and docetaxel) on S100A8/A9 gene expression profiles in four representative tissues (heart, kidney, liver, and lung) in normal C57BL/6J mice. Both S100A8/A9 expression was transiently or time-dependently elevated in four tissues within 48 h after dosing of the three anticancer agents under toxicity-inducing conditions. S100A8/A9 patterns differed among agents and tissues. This result suggests that S100A8/A9 is useful for evaluating anticancer agent-induced tissue damage. Metabolomic analysis revealed that some metabolites showed temporal patterns similar to that of S100A8/A9 expression. The amounts of fumarate (doxorubicin-treated heart), tyrosine (cisplatin-treated kidney), acetylcarnosine (doxorubicin-treated liver), and 2-phosphoglycerate (docetaxel-treated lung) showed similar patterns to that of S100A8/A9 expression. Although these metabolites showed different behaviors between tissues and serum, they may be useful marker candidates for evaluating anticancer agent-induced tissue damage at an earlier stage after dosing.

    DOI: 10.1016/j.fct.2022.113421

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  • Japanese soup stocks (katsuo-dashi and kombu-dashi) modulate food factor sensing-related gene expression in mice Reviewed

    Motoki Murata, Kai Nakayama, Ryo Kitamura, Megumi Goto, Mai Morozumi, Takanori Yoshimoto, Yuki Marugame, Ren Yoshitomi, Shuya Yamashita, Yoshinori Fujimura, Hirofumi Tachibana

    International Journal of Gastronomy and Food Science   29   100573 - 100573   2022.9   ISSN:1878-450X

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    DOI: 10.1016/j.ijgfs.2022.100573

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  • Circulating miRNA profiles in mice plasma following flavonoid intake Reviewed

    Motoki Murata, Yuki Marugame, Shuhei Yamada, Ichian Lin, Shuya Yamashita, Yoshinori Fujimura, Hirofumi Tachibana

    Molecular Biology Reports   49 ( 11 )   10399 - 10407   2022.9   ISSN:0301-4851 eISSN:1573-4978

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    DOI: 10.1007/s11033-022-07918-9

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  • 67-kDa Laminin Receptor-Mediated Cellular Sensing System of Green Tea Polyphenol EGCG and Functional Food Pairing. Invited Reviewed International journal

    Yoshinori Fujimura, Motofumi Kumazoe, Hirofumi Tachibana

    Molecules (Basel, Switzerland)   27 ( 16 )   5130   2022.8   eISSN:1420-3049

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    The body is equipped with a "food factor-sensing system" that senses food factors, such as polyphenols, sulfur-containing compounds, and vitamins, taken into the body, and plays an essential role in manifesting their physiological effects. For example, (-)-epigallocatechin-3-O-gallate (EGCG), the representative catechin in green tea (Camellia sinensi L.), exerts various effects, including anti-cancer, anti-inflammatory, and anti-allergic effects, when sensed by the cell surficial protein 67-kDa laminin receptor (67LR). Here, we focus on three representative effects of EGCG and provide their specific signaling mechanisms, the 67LR-mediated EGCG-sensing systems. Various components present in foods, such as eriodictyol, hesperetin, sulfide, vitamin A, and fatty acids, have been found to act on the food factor-sensing system and affect the functionality of other foods/food factors, such as green tea extract, EGCG, or its O-methylated derivative at different experimental levels, i.e., in vitro, animal models, and/or clinical trials. These phenomena are observed by increasing or decreasing the activity or expression of EGCG-sensing-related molecules. Such functional interaction between food factors is called "functional food pairing". In this review, we introduce examples of functional food pairings using EGCG.

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  • Fustin, a flavanonol, synergically potentiates the anti-cancer effect of the green tea catechin EGCG with activation of the eNOS/cGMP axis Reviewed International journal

    @Fujimura Y, #Watanabe M, @Morikawa-Ichinose T, #Fujino K, #Yamamoto M, #Nishioka S, #Inoue C, #Ogawa F, @Yonekura M, @Nakasone A, @Kumazoe M, @Tachibana H.

    J. Agric. Food Chem.   70 ( 21 )   6455 - 6466   2022.6

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    DOI: doi: 10.1021/acs.jafc.2c01693

  • Metabolic Profiling for Evaluating the Dipeptidyl Peptidase-IV Inhibitory Potency of Diverse Green Tea Cultivars and Determining Bioactivity-Related Ingredients and Combinations. Reviewed International journal

    Yoshinori Fujimura, Mototsugu Watanabe, Tomomi Morikawa-Ichinose, Konatsu Fujino, Mao Yamamoto, Seita Nishioka, Chihiro Inoue, Fumiyo Ogawa, Madoka Yonekura, Akari Nakasone, Motofumi Kumazoe, Hirofumi Tachibana

    Journal of Agricultural and Food Chemistry (Cover Selected)   70 ( 21 )   6455 - 6466   2022.6   ISSN:0021-8561 eISSN:1520-5118

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    There are numerous cultivars of tea (Camellia sinensis L.), but the differences in their anti-hyperglycemic-related effects are largely unknown. The inhibition of the dipeptidyl peptidase (DPP)-IV enzyme plays an essential role in controlling hyperglycemia in diabetes by blocking the degradation of incretin hormones, which is necessary for insulin secretion. In this study, we examined the DPP-IV inhibitory activity of leaf extracts from diverse Japanese green tea cultivars. The inhibitory rates differed among tea extracts. Metabolic profiling (MP), using liquid chromatography-mass spectrometry, of all cultivars revealed compositional differences among cultivars according to their DPP-IV inhibitory capacity. Epigallocatechin-3-O-(3-O-methyl)gallate, kaempferol-3-O-rutinoside, myricetin-3-O-glucoside/galactoside, and theogallin were newly identified as DPP-IV inhibitors. The bioactivity of a tea extract was potentiated by adding these ingredients in combination. Our results show that MP is a useful approach for evaluating the DPP-IV inhibitory potency of green tea and for determining bioactivity-related ingredients and combinations.

    DOI: 10.1021/acs.jafc.2c01693

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  • Myricetin improves cognitive function in SAMP8 mice and upregulates brain derived neurotrophic factor and nerve growth factor Reviewed International journal

    Shimada Y, Sato Y, Kumazoe M, Kitamura R, Fujimura Y, Tachibana H.

    Biochem. Biophys. Res. Commun.   616   33 - 40   2022.5

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    DOI: doi: 10.1016/j.bbrc.2022.05.039

  • Fustin, a Flavanonol, Synergically Potentiates the Anticancer Effect of Green Tea Catechin Epigallocatechin-3-<i>O</i>-Gallate with Activation of the eNOS/cGMP Axis Reviewed

    Motofumi Kumazoe, Yoshinori Fujimura (Equal contribution), Ren Yoshitomi, Yu Shimada, Hirofumi Tachibana

    Journal of Agricultural and Food Chemistry (Cover Selected)   70 ( 11 )   3458 - 3466   2022.3   ISSN:0021-8561 eISSN:1520-5118

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    DOI: 10.1021/acs.jafc.1c07567

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  • Sesame lignans upregulate glutathione S-transferase expression and downregulate microRNA-669c-3p Reviewed International journal

    Marugame Y, Takeshita N, Yamada S, Yoshitomi R, Kumazoe M, Fujimura Y, Tachibana H.

    Bioscience of Microbiota, Food and Health   41 ( 2 )   66 - 72   2022.1

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    DOI: doi: 10.12938/bmfh.2021-067

  • Methylated (−)-epigallocatechin 3-O-gallate potentiates the effect of split vaccine accompanied with upregulation of Toll-like receptor 5 Reviewed International journal

    11 ( 1 )   23101 - 1042   2021.11

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    DOI: doi: 10.1038/s41598-021-02346-4

  • The combined effect of green tea and α-glucosyl hesperidin in preventing obesity: a randomized placebo-controlled clinical trial. Reviewed International journal

    11 ( 1 )   19067 - 19067   2021.9

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    Green tea, a widely consumed beverage in Asia, contains green tea catechins effective against obesity, especially epigallocatechin-3-O-gallate (EGCG), but must be consumed in an impractically huge amount daily to elicit its biological effect. Meanwhile, citrus polyphenols have various physiological effects that could enhance EGCG functionality. Here we investigated the antiobesity effect of a combination of EGCG and α-glucosyl hesperidin, a citrus polyphenol, at doses that have not been previously reported to exert antiobesity effects by themselves in any clinical trial. In a randomized, placebo-controlled, double-blinded, and parallel-group-designed clinical trial, 60 healthy Japanese males and females aged 30-75 years consumed green tea combined with α-glucosyl hesperidin (GT-gH), which contained 178 mg α-glucosyl hesperidin and 146 mg EGCG, for 12 weeks. Physical, hematological, blood biochemical, and urine examinations showed that GT-gH is safe to use. At week 12, GT-gH prevented weight gain and reduced body mass index (BMI) compared with the placebo. Especially in those aged < 50 years, triglyceride and body fat percentage decreased at week 6, visceral fat level and body fat percentage decreased at week 12; body weight, BMI, and blood LDL/HDL ratio also decreased. In conclusion, taking GT-gH prevents weight gain, and the antiobesity effect of GT-gH was more pronounced in people aged < 50 years.

    DOI: 10.1038/s41598-021-98612-6

  • Time-dependent increase of plasma cGMP concentration followed by oral EGCG administration in mice Invited Reviewed International journal

    Tanaka Y, Kumazoe M, Onda H, Fujimura Y, Tachibana H.

    Food Biosci   41   2021.6

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  • Eriodictyol-Amplified 67-kDa Laminin Receptor Signaling Potentiates the Antiallergic Effect of O-Methylated Catechin. Reviewed International journal

    Yoshinori Fujimura, Konatsu Fujino, Takanori Yoshimoto, Ayaka Nezu, Yuki Marugame, Jaehoon Bae, Motofumi Kumazoe, Hirofumi Tachibana

    Journal of Natural Products (Cover Selected)   84 ( 6 )   1823 - 1830   2021.6

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    DOI: 10.1021/acs.jnatprod.1c00337

  • Glucosyl-hesperidin enhances the cyclic guanosine monophosphate-inducing effect of a green tea polyphenol EGCG. Reviewed International journal

    Kumazoe M, Tanaka Y, Yoshitomi R, Marugame Y, Lee KW, Onda H, Fujimura Y, Yonekura M, Shimamoto Y, Tachibana H.

    J Nat Med   75   1037 - 1042   2021.6

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    DOI: doi: 10.1007/s11418-021-01538-6

  • Plasma Homocysteine Concentration is Associated with the Expression Level of Folate Receptor 3 Reviewed

    Ren Yoshitomi, Kai Nakayama, Shuya Yamashita, Motofumi Kumazoe, Ting-An Lin, Chen-Yi Mei, Yuki Marugame, Yoshinori Fujimura, Mari Maeda-Yamamoto, Shinichi Kuriyama, Hirofumi Tachibana

    Scientific Reports   10 ( 1 )   2020.12

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    DOI: 10.1038/s41598-020-67288-9

  • Src mediates epigallocatechin-3-O-gallate-elicited acid sphingomyelinase activation Reviewed International journal

    Kumazoe M, Kadomatsu M, Bae J, Otsuka Y, Fujimura Y, Tachibana H

    Molecules   25 ( 22 )   5481   2020.11

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    DOI: 10.3390/molecules25225481

  • 67-kDa Laminin Receptor mediates the beneficial effects of Green tea polyphenol EGCG Reviewed

    Kumazoe M, Fujimura Y, Tachibana H

    Curr. Pharmacol. Rep   6   280 - 285   2020.7

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  • Plasma Homocysteine Concentration is Associated with the Expression Level of Folate Receptor 3 Reviewed International journal

    Yoshitomi R, Nakayama K, Yamashita S, Kumazoe M, Lin TA, Mei CY, Marugame Y, Fujimura Y, Maeda-Yamamoto M, Kuriyama S, *Tachibana H

    Sci Rep   10 ( 1 )   2020.6

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    DOI: 10.1038/s41598-020-67288-9

  • Cancer cell selective probe by mimicking EGCG. Reviewed International journal

    Motofumi Kumazoe, Shun Hiroi, Yousuke Tanimoto, Jyunichi Miyakawa, Maasa Yamanouchi, Yumi Suemasu, Ren Yoshitomi, Motoki Murata, Yoshinori Fujimura, Takashi Takahashi, Hiroshi Tanaka, Hirofumi Tachibana

    Biochemical and Biophysical Research Communications   525 ( 4 )   974 - 981   2020.5

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    DOI: 10.1016/j.bbrc.2020.03.021

  • EGCG down-regulates MuRF1 expression through 67-kDa laminin receptor and the receptor signaling is amplified by eriodictyol. Reviewed

    Motoki Murata, Yuki Shimizu, Yuki Marugame, Ayaka Nezu, Konatsu Fujino, Shuhei Yamada, Motofumi Kumazoe, Yoshinori Fujimura, Hirofumi Tachibana

    Journal of Natural Medicines   74 ( 4 )   673 - 679   2020.5

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    DOI: 10.1007/s11418-020-01417-6

  • Procyanidin C1 Inhibits Melanoma Cell Growth by Activating 67-kDa Laminin Receptor Signaling Reviewed International journal

    Bae J, Kumazoe M, Murata K, Fujimura Y, Tachibana H.

    Mol. Nutr. Food Res. (Cover Selected)   64 ( 7 )   e1900986   2020.4

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    DOI: 10.1002/mnfr.201900986

  • Epigallocatechin-3-O-gallate induces acid sphingomyelinase activation through activation of phospholipase C Reviewed International journal

    Bae J, Kumazoe M, Takeuchi C, Hidaka S, Fujimura Y, Tachibana H.

    Biochem. Biophys. Res. Commun.   520 ( 1 )   186 - 191   2019.11

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    DOI: 10.1016/j.bbrc.2019.09.102

  • Cuprizone-treated mice, a possible model of schizophrenia, highlighting the simultaneous abnormalities of GABA, serine and glycine in hippocampus. Reviewed

    Hayakawa E, Ohgidani M, Fujimura Y, Kanba S, Miura D, Kato TA

    Schizophrenia Research   210   326 - 328   2019.8

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    Cuprizone-treated mice, a possible model of schizophrenia, highlighting the simultaneous abnormalities of GABA, serine and glycine in hippocampus.

    DOI: 10.1016/j.schres.2019.06.010

  • Improvement of Sensitivity and Reproducibility for Imaging of Endogenous Metabolites by Matrix-Assisted Laser Desorption/Ionization-Mass Spectrometry. Reviewed

    Morikawa-Ichinose T, Fujimura Y, Murayama F, Yamazaki Y, Yamamoto T, Wariishi H, Miura D

    Journal of the American Society for Mass Spectrometry   2019.5

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    Improvement of Sensitivity and Reproducibility for Imaging of Endogenous Metabolites by Matrix-Assisted Laser Desorption/Ionization-Mass Spectrometry.

    DOI: 10.1007/s13361-019-02221-7

  • Diallyl disulfide potentiates anti-obesity effect of green tea in high-fat/high-sucrose diet-induced obesity Reviewed International journal

    Bae J, Kumazoe M, Fujimura Y, Tachibana H

    J Nutr Biochem   64   152 - 161   2019.2

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    DOI: 10.1016/j.jnutbio.2018.10.014

  • Postprandial glycaemia-lowering effect of a green tea cultivar Sunrouge and cultivar-specific metabolic profiling for determining bioactivity-related ingredients. Reviewed International journal

    Masafumi Wasai, Yoshinori Fujimura, Haruna Nonaka, Ryo Kitamura, Motoki Murata, Hirofumi Tachibana

    Scientific Reports   8 ( 1 )   16041 - 16041   2018.10

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    DOI: 10.1038/s41598-018-34316-8

  • Visualizing Energy Charge in Breast Carcinoma Tissues by MALDI Mass-spectrometry Imaging Profiles of Low-molecular-weight Metabolites. Reviewed

    Torata N, Kubo M, Miura D, Ohuchida K, Mizuuchi Y, Fujimura Y, Hayakawa E, Kai M, Oda Y, Mizumoto K, Hashizume M, Nakamura M

    Anticancer Research   38 ( 7 )   4267 - 4272   2018.7

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    Visualizing Energy Charge in Breast Carcinoma Tissues by MALDI Mass-spectrometry Imaging Profiles of Low-molecular-weight Metabolites.

    DOI: 10.21873/anticanres.12723

  • Analysis of spatiotemporal metabolomic dynamics for sensitively monitoring biological alterations in cisplatin-induced acute kidney injury Reviewed

    Miho Irie, Eisuke Hayakawa, Yoshinori Fujimura, Youhei Honda, Daiki Setoyama, Hiroyuki Wariishi, Fuminori Hyodo, Daisuke Miura

    Biochemical and Biophysical Research Communications   496 ( 1 )   140 - 146   2018.1

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    DOI: 10.1016/j.bbrc.2018.01.012

  • A Phytochemical-Sensing Strategy Based on Mass Spectrometry Imaging and Metabolic Profiling for Understanding the Functionality of the Medicinal Herb Green Tea Reviewed

    Yoshinori Fujimura, Daisuke Miura, Hirofumi Tachibana

    MOLECULES   22 ( 10 )   2017.10

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    DOI: 10.3390/molecules22101621

  • A Chemometrics-driven Strategy for the Bioactivity Evaluation of Complex Multicomponent Systems and the Effective Selection of Bioactivity-predictive Chemical Combinations Reviewed

    Yoshinori Fujimura, Chihiro Kawano, Ayaka Maeda-Murayama, Asako Nakamura, Akiko Koike-Miki, Daichi Yukihira, Eisuke Hayakawa, Takanori Ishii, Hirofumi Tachibana, Hiroyuki Wariishi, Daisuke Miura

    SCIENTIFIC REPORTS   7   2017.5

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    DOI: 10.1038/s41598-017-02499-1

  • Spatially resolved metabolic distribution for unraveling the physiological change and responses in tomato fruit using matrix-assisted laser desorption/ionization–mass spectrometry imaging (MALDI–MSI)

    409 ( 6 )   1697 - 1706   2017.2

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    © 2016, The Author(s). Information on spatiotemporal metabolic behavior is indispensable for a precise understanding of physiological changes and responses, including those of ripening processes and wounding stress, in fruit, but such information is still limited. Here, we visualized the spatial distribution of metabolites within tissue sections of tomato (Solanum lycopersicum L.) fruit using a matrix-assisted laser desorption/ionization–mass spectrometry imaging (MALDI–MSI) technique combined with a matrix sublimation/recrystallization method. This technique elucidated the unique distribution patterns of more than 30 metabolite-derived ions, including primary and secondary metabolites, simultaneously. To investigate spatiotemporal metabolic alterations during physiological changes at the whole-tissue level, MALDI–MSI was performed using the different ripening phenotypes of mature green and mature red tomato fruits. Although apparent alterations in the localization and intensity of many detected metabolites were not observed between the two tomatoes, the amounts of glutamate and adenosine monophosphate, umami compounds, increased in both mesocarp and locule regions during the ripening process. In contrast, malate, a sour compound, decreased in both regions. MALDI–MSI was also applied to evaluate more local metabolic responses to wounding stress. Accumulations of a glycoalkaloid, tomatine, and a low level of its glycosylated metabolite, esculeoside A, were found in the wound region where cell death had been induced. Their inverse levels were observed in non-wounded regions. Furthermore, the amounts of both compounds differed in the developmental stages. Thus, our MALDI–MSI technique increased the understanding of the physiological changes and responses of tomato fruit through the determination of spatiotemporally resolved metabolic alterations. [Figure not available: see fulltext.]

    DOI: 10.1007/s00216-016-0118-4

  • Oligomer formation of a tea polyphenol, EGCG, on its sensing molecule 67 kDa laminin receptor Reviewed

    Yuhui Huang, Mami Sumida, Motofumi Kumazoe, Kaori Sugihara, Yumi Suemasu, Shuhei Yamada, Shuya Yamashita, Jyunichi Miyakawa, Takashi Takahashi, Hiroshi Tanaka, Yoshinori Fujimura, Hirofumi Tachibana

    CHEMICAL COMMUNICATIONS (Cover Selected)   53 ( 12 )   1941 - 1944   2017.2

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    DOI: 10.1039/c6cc09504f

  • MSIdV: a versatile tool to visualize biological indices from mass spectrometry imaging data Reviewed

    Eisuke Hayakawa, Yoshinori Fujimura, Daisuke Miura

    BIOINFORMATICS   32 ( 24 )   3852 - 3854   2016.12

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    DOI: 10.1093/bioinformatics/btw548

  • Sphingosine Kinase-1 Protects Multiple Myeloma from Apoptosis Driven by Cancer-Specific Inhibition of RTKs Reviewed International journal

    Tsukamoto S, Huang Y, Kumazoe M, Lesnick C, Yamada S, Ueda N, Suzuki T, Yamashita S, Kim YH, Fujimura Y, Miura D, Kay NE, Shanafelt TD, Tachibana H

    Mol Cancer Ther   14   2303 - 2312   2015.10

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    DOI: 10.1158/1535-7163

  • Bacterial metabolism in immediate response to nutritional perturbation with temporal and network view of metabolites Reviewed International journal

    Yukihira D, Fujimura Y, Wariishi H, Miura D

    Mol Biosyst   11   2473 - 2482   2015.9

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    DOI: 10.1039/c5mb00182j

  • Sphingosine kinase-1 protects B-cell neoplasm and myeloid leukemia from apoptosis driven by cancer specific inhibition of RTKs. Reviewed International journal

    Shuntaro Tsukamoto, Yuhui Huang, Motofumi Kumazoe, Connie Lesnick, Suhei Yamada, Naoki Ueda, Takashi Suzuki, Shuya Yamashita, Yoon Hee Kim, Yoshinori Fujimura, Daisuke Miura, Nail E. Kay, Tait D Shanafelt, Hirofumi Tachibana

    Molecular Cancer Therapeutics   14 ( 10 )   1162 - 1168   2015.9

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    Sphingosine kinase-1 protects B-cell neoplasm and myeloid leukemia from apoptosis driven by cancer specific inhibition of RTKs.
    Activation of acid sphingomyelinase (ASM) leads to ceramide accumulation and induces apoptotic cell death in cancer cells. In the present study, we demonstrate that the activation of ASM by targeting cancer-overexpressed 67-kDa laminin receptors (67LR) induces lipid raft disruption and inhibits receptor tyrosine kinase (RTK) activation in multiple myeloma cells. Sphingosine kinase 1 (SphK1), a negative regulator of ceramide accumulation with antiapoptotic effects, was markedly elevated in multiple myeloma cells. The silencing of SphK1 potentiated the apoptotic effects of the green tea polyphenol epigallocatechin-3-O-gallate (EGCG), an activator of ASM through 67LR. Furthermore, the SphK1 inhibitor safingol synergistically sensitized EGCG-induced proapoptotic cell death and tumor suppression in multiple myeloma cells by promoting the prevention of RTK phosphorylation and activation of death-associated protein kinase 1 (DAPK1). We propose that targeting 67LR/ASM and SphK1 may represent a novel therapeutic strategy against multiple myeloma.

    DOI: 10.1158/1535-7163.MCT-15-0185

  • Small molecule-sensing strategy and techniques for understanding the functionality of green tea Invited Reviewed

    Yoshinori Fujimura

    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY   79 ( 5 )   687 - 699   2015.5

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    DOI: 10.1080/09168451.2014.996205

  • GLP-1 analog liraglutide protects against cardiac steatosis, oxidative stress and apoptosis in streptozotocin-induced diabetic rats Reviewed

    Tomoaki Inoue, Toyoshi Inoguchi, Noriyuki Sonoda, Hari Hendarto, Hiroaki Makimura, Shuji Sasaki, Hisashi Yokomizo, Yoshinori Fujimura, Daisuke Miura, Ryoichi Takayanagi

    ATHEROSCLEROSIS   240 ( 1 )   250 - 259   2015.5

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    DOI: 10.1016/j.atherosclerosis.2015.03.026

  • Identification of 3-Methylbutanoyl Glycosides in Green Coffea arabica Beans as Causative Determinants for the Quality of Coffee Flavors Reviewed

    Keiko Iwasa, Daiki Setoyama, Hiroaki Shimizu, Harumichi Seta, Yoshinori Fujimura, Daisuke Miura, Hiroyuki Wariishi, Chifumi Nagai, Koichi Nakahara

    JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY   63 ( 14 )   3742 - 3751   2015.4

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    DOI: 10.1021/jf5054047

  • Metabolic Profiling-based Data-mining for an Effective Chemical Combination to Induce Apoptosis of Cancer Cells Reviewed

    Motofumi Kumazoe, Yoshinori Fujimura, Shiori Hidaka, Yoonhee Kim, Kanako Murayama, Mika Takai, Yuhui Huang, Shuya Yamashita, Motoki Murata, Daisuke Miura, Hiroyuki Wariishi, Mari Maeda-Yamamoto, Hirofumi Tachibana

    SCIENTIFIC REPORTS   5   2015.3

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    DOI: 10.1038/srep09474

  • In Situ Label-Free Visualization of Orally Dosed Strictinin within Mouse Kidney by MALDI-MS Imaging Reviewed

    Yoon Hee Kim, Yoshinori Fujimura, Masako Sasaki, Xue Yang, Daichi Yukihira, Daisuke Miura, Yumi Unno, Koretsugu Ogata, Hiroki Nakajima, Shuya Yamashita, Kanami Nakahara, Motoki Murata, I-Chian Lin, Hiroyuki Wariishi, Koji Yamada, Hirofumi Tachibana

    JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY   62 ( 38 )   9279 - 9285   2014.9

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    DOI: 10.1021/jf503143g

  • IL-4 receptor α in non-lipid rafts is the target molecule of strictinin in inhibiting STAT6 activation Reviewed

    450 ( 1 )   824 - 830   2014.7

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    Strictinin has been shown to suppress interleukin (IL)-4-induced signal transducer and activator of transcription (STAT)-6 phosphorylation, which is a critical event for IgE class switching. However, it is unclear how strictinin inhibits STAT6 activation. Strictinin inhibited STAT6 phosphorylation by suppressing IL-4 receptor α (IL-4Rα) activation. Strictinin was bound to the cell surface and only localized in non-lipid raft fraction of the cells where IL-4Rα was also located. In addition, strictinin directly bound to IL-4Rα and inhibited binding of IL-4 to IL-4Rα. These results suggest that IL-4Rα locating in non-lipid raft region is a target molecule for strictinin in inhibiting STAT6 activation. © 2014 Elsevier Inc. All rights reserved.

    DOI: 10.1016/j.bbrc.2014.06.069

  • Integrated MALDI-MS imaging and LC-MS techniques for visualizing spatiotemporal metabolomic dynamics in a rat stroke model Reviewed

    Miho Irie, Yoshinori Fujimura, Mayumi Yamato, Daisuke Miura, Hiroyuki Wariishi

    METABOLOMICS   10 ( 3 )   473 - 483   2014.6

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    DOI: 10.1007/s11306-013-0588-8

  • MALDI Mass Spectrometry Imaging for Visualizing In Situ Metabolism of Endogenous Metabolites and Dietary Phytochemicals. Reviewed

    Fujimura Y, Miura D

    Metabolites   4 ( 2 )   319 - 346   2014.5

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    MALDI Mass Spectrometry Imaging for Visualizing In Situ Metabolism of Endogenous Metabolites and Dietary Phytochemicals.

    DOI: 10.3390/metabo4020319

  • Mass Spectrometry-Based Metabolic Profiling of Gemcitabine-Sensitive and Gemcitabine-Resistant Pancreatic Cancer Cells Reviewed

    Yoshinori Fujimura, Naoki Ikenaga, Kenoki Ohuchida, Daiki Setoyama, Miho Irie, Daisuke Miura, Hiroyuki Wariishi, Masaharu Murata, Kazuhiro Mizumoto, Makoto Hashizume, Masao Tanaka

    PANCREAS   43 ( 2 )   311 - 318   2014.3

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    DOI: 10.1097/MPA.0000000000000092

  • Power of isotopic fine structure for unambiguous determination of metabolite elemental compositions: In silico evaluation and metabolomic application Reviewed

    Tatsuhiko Nagao, Daichi Yukihira, Yoshinori Fujimura, Kazunori Saito, Katsutoshi Takahashi, Daisuke Miura, Hiroyuki Wariishi

    ANALYTICA CHIMICA ACTA   813   70 - 76   2014.2

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    DOI: 10.1016/j.aca.2014.01.032

  • A QSPR Study on Structural Properties of Metabolites for Preferred Ionization in MALDI-MS Analysis Reviewed International journal

    Yukihira D, Miura D, Fujimura Y, Umemura Y, Yamaguchi S, Funatsu S, Yamazaki M, Ohta T, Inoue H, Shindo M, Wariishi H

    J. Am. Soc. Mass Spectrom.   25   1 - 5   2014.1

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  • MALDI Efficiency of Metabolites Quantitatively Associated with their Structural Properties: A Quantitative Structure-Property Relationship (QSPR) Approach Reviewed

    Daichi Yukihira, Daisuke Miura, Yoshinori Fujimura, Yoshikatsu Umemura, Shinichi Yamaguchi, Shinji Funatsu, Makoto Yamazaki, Tetsuya Ohta, Hiroaki Inoue, Mitsuru Shindo, Hiroyuki Wariishi

    JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY (Cover Selected)   25 ( 1 )   1 - 5   2014.1

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    DOI: 10.1007/s13361-013-0772-0

  • Metformin and liraglutide ameliorate high glucose-induced oxidative stress via inhibition of PKC-NAD(P)H oxidase pathway in human aortic endothelial cells Reviewed

    Battsetseg Batchuluun, Toyoshi Inoguchi, Noriyuki Sonoda, Shuji Sasaki, Tomoaki Inoue, Yoshinori Fujimura, Daisuke Miura, Ryoichi Takayanagi

    ATHEROSCLEROSIS   232 ( 1 )   156 - 164   2014.1

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    DOI: 10.1016/j.atherosclerosis.2013.10.025

  • Metabolomics reveals that carnitine palmitoyltransferase-1 is a novel target for oxidative inactivation in human cells Reviewed

    Daiki Setoyama, Yoshinori Fujimura, Daisuke Miura

    Genes to Cells   18 ( 12 )   1107 - 1119   2013.12

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    DOI: 10.1111/gtc.12098

  • In situ label-free imaging for visualizing the biotransformation of a bioactive polyphenol Reviewed

    Yoon Hee Kim, Yoshinori Fujimura, Takatoki Hagihara, Masako Sasaki, Daichi Yukihira, Tatsuhiko Nagao, Daisuke Miura, Shinichi Yamaguchi, Kazunori Saito, Hiroshi Tanaka, Hiroyuki Wariishi, Koji Yamada, Hirofumi Tachibana

    SCIENTIFIC REPORTS   3   2013.9

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    DOI: 10.1038/srep02805

  • High-Throughput Metabolic Profiling of Diverse Green Coffea arabica Beans Identified Tryptophan as a Universal Discrimination Factor for Immature Beans Reviewed

    Daiki Setoyama, Keiko Iwasa, Harumichi Seta, Hiroaki Shimizu, Yoshinori Fujimura, Daisuke Miura, Hiroyuki Wariishi, Chifumi Nagai, Koichi Nakahara

    PLOS ONE   8 ( 8 )   2013.8

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    DOI: 10.1371/journal.pone.0070098

  • Tannic acid, a higher galloylated pentagalloylglucose, suppresses antigen-specific IgE production by inhibiting ε germline transcription induced by STAT6 activation Reviewed International journal

    3   341 - 345   2013.8

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  • Downregulation of adipose triglyceride lipase in the heart aggravates diabetic cardiomyopathy in db/db mice Reviewed

    Tomoaki Inoue, Kunihisa Kobayashi, Toyoshi Inoguchi, Noriyuki Sonoda, Yasutaka Maeda, Eiichi Hirata, Yoshinori Fujimura, Daisuke Miura, Ken-ichi Hirano, Ryoichi Takayanagi

    Biochemical and Biophysical Research Communications   438 ( 1 )   224 - 229   2013.8

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    DOI: 10.1016/j.bbrc.2013.07.063

  • In situ metabolomic mass spectrometry imaging: Recent advances and difficulties Reviewed

    Daisuke Miura, Yoshinori Fujimura, Hiroyuki Wariishi

    JOURNAL OF PROTEOMICS   75 ( 16 )   5052 - 5060   2012.8

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    DOI: 10.1016/j.jprot.2012.02.011

  • Green Tea Polyphenol EGCG Sensing Motif on the 67-kDa Laminin Receptor Reviewed

    Yoshinori Fujimura, Mami Sumida, Kaori Sugihara, Shuntaro Tsukamoto, Koji Yamada, Hirofumi Tachibana

    PLOS ONE   7 ( 5 )   2012.5

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    DOI: 10.1371/journal.pone.0037942

  • Reduced expression of adipose triglyceride lipase enhances tumor necrosis factor α-induced intercellular adhesion molecule-1 expression in human aortic endothelial cells via protein kinase C-dependent activation of nuclear factor-κB Reviewed

    286 ( 37 )   32045 - 32053   2011.9

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    We examined the effects of adipose triglyceride lipase (ATGL) on the initiation of atherosclerosis. ATGL was recently identified as a rate-limiting triglyceride (TG) lipase. Mutations in the human ATGL gene are associated with neutral lipid storage disease with myopathy, a rare genetic disease characterized by excessive accumulation of TG in multiple tissues. The cardiac phenotype, known as triglyceride deposit cardiomyovasculopathy, shows massive TG accumulation in both coronary atherosclerotic lesions and the myocardium. Recent reports show that myocardial triglyceride content is significantly higher in patients with prediabetes or diabetes and that ATGL expression is decreased in the obese insulin-resistant state. Therefore, we investigated the effect of decreased ATGL activity on the development of atherosclerosis using human aortic endothelial cells. We found that ATGL knockdown enhanced monocyte adhesion via increased expression of TNFα-induced intercellular adhesion molecule-1 (ICAM-1). Next, we determined the pathways (MAPK, PKC, or NFκB) involved in ICAM-1 up-regulation induced by ATGL knockdown. Both phosphorylation of PKC and degradation of IκBα were increased in ATGL knockdown human aortic endothelial cells. In addition, intracellular diacylglycerol levels and free fatty acid uptake via CD36 were significantly increased in these cells. Inhibition of the PKC pathway using calphostin C and GF109203X suppressed TNFα-induced ICAM-1 expression. In conclusion, we showed that ATGL knockdown increased monocyte adhesion to the endothelium through enhanced TNFα-induced ICAM-1 expression via activation of NFκB and PKC. These results suggest that reduced ATGL expression may influence the atherogenic process in neutral lipid storage diseases and in the insulin-resistant state. © 2011 by The American Society for Biochemistry and Molecular Biology, Inc.

    DOI: 10.1074/jbc.M111.285650

  • Metabolomics-Driven Nutraceutical Evaluation of Diverse Green Tea Cultivars Reviewed

    Yoshinori Fujimura, Kana Kurihara, Megumi Ida, Reia Kosaka, Daisuke Miura, Hiroyuki Wariishi, Mari Maeda-Yamamoto, Atsushi Nesumi, Takeshi Saito, Tomomasa Kanda, Koji Yamada, Hirofumi Tachibana

    PLOS ONE   6 ( 8 )   2011.8

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    DOI: 10.1371/journal.pone.0023426

  • Ultrahighly Sensitive in Situ Metabolomic Imaging for Visualizing Spatiotemporal Metabolic Behaviors Reviewed

    Miura, Daisuke, Fujimura, Yoshinori, Yamato, Mayumi, Hyodo, Fuminori, Utsumi, Hideo, Tachibana, Hirofumi, Wariishi, Hiroyuki

    ANALYTICAL CHEMISTRY   82 ( 23 )   9789 - 9796   2010.12

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    DOI: 10.1021/ac101998z

  • TLR4 Signaling Inhibitory Pathway Induced by Green Tea Polyphenol Epigallocatechin-3-Gallate through 67-kDa Laminin Receptor Reviewed

    Eui Hong Byun, Yoshinori Fujimura, Koji Yamada, Hirofumi Tachibana

    JOURNAL OF IMMUNOLOGY   185 ( 1 )   33 - 45   2010.7

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    DOI: 10.4049/jimmunol.0903742

  • Comparisons of uptake and cell surface binding among pyridoxal, pyridoxine, and pyridoxamine in RAW264.7 cells Reviewed

    Hiroaki Kanouchi, Mayumi Shibuya, Shuntaro Tsukamoto, Yoshinori Fujimura, Hirofumi Tachibana, Koji Yamada, Tatsuzo Oka

    NUTRITION   26 ( 6 )   648 - 652   2010.6

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    DOI: 10.1016/j.nut.2009.08.005

  • Maternal exposure to dioxin reduces hypothalamic but not pituitary metabolome in fetal rats: a possible mechanism for a fetus-specific reduction in steroidogenesis Reviewed

    Yuki Matsumoto, Takumi Ishida, Tomoki Takeda, Takayuki Koga, Misaki Fujii, Yuji Ishii, Yoshinori Fujimura, Daisuke Miura, Hiroyuki Wariishi, Hideyuki Yamada

    JOURNAL OF TOXICOLOGICAL SCIENCES   35 ( 3 )   365 - 373   2010.6

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    DOI: 10.2131/jts.35.365

  • Functional evaluation of anticancer drugs in human leukemia cells based on metabolic profiling technique International journal

    Miura, D., Fujimura, Y., Tachibana, H., and Wariishi, H.

    Animal Cell Technology   2010.5

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  • Highly Sensitive Matrix-Assisted Laser Desorption Ionization-Mass Spectrometry for High-Throughput Metabolic Profiling

    Daisuke Miura, Yoshinori Fujimura, Hirofumi Tachibana, Hiroyuki Wariishi

    ANALYTICAL CHEMISTRY   82 ( 2 )   498 - 504   2010.1

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    DOI: 10.1021/ac901083a

  • Involvement of 67 kDa laminin receptor on cellular uptake of green tea polyphenol, epigallocatechin-3-O-gallate, in Caco2 cells International journal

    Ohta, S., Fujimura, Y., Yamada, K., and Tachibana, H.

    Animal Cell Technology   15   2009.11

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  • Evaluation of cisplatin-induced nephrotoxicity by Overhauser MRI and mass spectrometry imaging Reviewed International journal

    Fujimura, Y., Miura, D., Hyodo, F., Yasukawa, K., Tachibana, H., Utsumi, H., and Wariishi, H

    Free Radic. Biol. Med.   47   2009.11

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  • In situ metabolomics imaging of a rat brain section of transient middle cerebral artery occlusion model Reviewed International journal

    Miura, D., Yamato, M., Fujimura, Y., Hyodo, F., Tachibana, H., Utsumi, H., and Wariishi, H.

    Free Radic. Biol. Med.   47   2009.11

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  • Visualization of nitroxyl probes for molecular redox imaging by Overhauser MRI and mass spectrometry imaging Reviewed International journal

    Hyodo, F., Miura, D., Fujimura, Y., Yasukawa, K., Sakai, K., Ichikawa, K., Wariishi, H., and Utsumi, H.

    Free Radic. Biol. Med.   47   2009.11

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  • Modulation of proliferation and differentiation of C2C12 skeletal muscle cells by fatty acids

    Ju-Hye Lee, Hirofumi Tachibana, Yoshiko Morinaga, Yoshinori Fujimura, Koji Yamada

    LIFE SCIENCES   84 ( 13-14 )   415 - 420   2009.3

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    DOI: 10.1016/j.lfs.2009.01.004

  • Relationsip between the biological activities of methylated derivatives of EGCG and their cell surface binding activities Reviewed International journal

    Yano, S., Fujimura, Y., Umeda, D., Miyase, T., Yamada, K., and Tachibana, H.

    J. Clin Biochem. Nutr.   43   2008.11

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  • The 67 kDa laminin receptor mediates anti-allergic effects of (-)-epigallocatechin-3-O-(3-O-methyl) gallate Reviewed International journal

    Fujimura, Y., Umeda, D., Maeda-Yamamoto, M., Yamada, K., and Tachibana, H.

    J. Clin Biochem. Nutr.   43   2008.10

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  • The impact of the 67 kDa laminin receptor on both cell-surface binding and anti-allergic action of tea catechins Reviewed

    Yoshinori Fujimura, Daisuke Umeda, Koji Yamada, Hirofumi Tachibana

    ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS   476 ( 2 )   133 - 138   2008.8

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    DOI: 10.1016/j.abb.2008.03.002

  • The 67 kDa laminin receptor as a primary determinant of anti-allergic effects of O-methylated EGCG Reviewed

    Yoshinori Fujimura, Daisuke Umeda, Satomi Yano, Mari Maeda-Yamamoto, Koji Yamada, Hirofumi Tachibana

    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS   364 ( 1 )   79 - 85   2007.12

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    DOI: 10.1016/j.bbrc.2007.09.095

  • Dietary flavones suppresses IgE and th2 cytokines in OVA-immunized BALB/c mice Reviewed

    Satomi Yano, Daisuke Umeda, Tatsunori Yamashita, Yu Ninomiya, Mami Sumida, Yoshinori Fujimura, Koji Yamada, Hirofumi Tachibana

    EUROPEAN JOURNAL OF NUTRITION   46 ( 5 )   257 - 263   2007.8

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    DOI: 10.1007/s00394-007-0658-7

  • Relationship between the biological activities of methylated derivatives of (-)-epigallocatechin-3-O-gallate (EGCG) and their cell surface binding activities Reviewed

    Satomi Yano, Yoshinori Fujimura, Daisuke Umeda, Toshio Miyase, Koji Yamada, Hirofumi Tachibana

    JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY   55 ( 17 )   7144 - 7148   2007.8

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    DOI: 10.1021/jf071176o

  • Aggregated ursolic acid, a natural triterpenoid, binds to CD36 for inducing interleukin-1 release from murine peritoneal macrophages Reviewed International journal

    41   2007.5

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  • Identification of the binding site of the green tea polyphenol EGCG receptor Reviewed International journal

    Tachibana, H., Fujimura, Y., Ogawa, N., Sumida, M., Tsuruda, S., and Yamada, K.

    J. Clin Biochem. Nutr.   41   2007.5

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  • Aggregated ursolic acid, a natural triterpenoid, induces interleukin-1 release in murine peritoneal macrophages: Role of CD36 Reviewed International journal

    178   2007.5

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  • Green tea polyphenol EGCG signaling through 67 kDa laminin receptor Reviewed International journal

    Tachibana, H., Daisuke, U., Fujimura, Y., and Yamada, K.

    J. Clin Biochem. Nutr.   41   2007.5

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  • The 67 kDa laminin receptor mediates anti-allergic effects of (-)-epigallocatechin-3-O-(3-O-methyl) gallate Reviewed International journal

    Fujimura, Y., Umeda, D., Maeda-Yamamoto, M., Yamada, K., and Tachibana, H.

    J. Clin Biochem. Nutr.   41   2007.5

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  • The involvement of the 67 kDa laminin receptor-mediated modulation of cytoskeleton in the degranulation inhibition induced by epigallocatechin-3-O-gallate Reviewed

    Yoshinori Fujimura, Daisuke Umeda, Yuko Kiyohara, Yousuke Sunada, Koji Yamada, Hirofumi Tachibana

    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS   348 ( 2 )   524 - 531   2006.9

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    DOI: 10.1016/j.bbrc.2006.07.086

  • Dietary apigenin suppresses IgE and inflammatory cytokines production in C57BL/6N mice Reviewed

    S Yano, D Umeda, N Maeda, Y Fujimura, K Yamada, H Tachibana

    JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY   54 ( 14 )   5203 - 5207   2006.7

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    DOI: 10.1021/jf0607361

  • 不知火姫菊抽出物のTNF-alpha 産生促進効果 Reviewed

    児林聡美, 小川直人, 藤村由紀, 立花宏文, 山田耕路

    日本食品科学工学会誌   53 ( 8 )   2006.5

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  • Water-soluble component in dried chrysanthemum flower stimulates tumor necrosis factor-α production by mouse macrophage-like cell line RAW264.7 Reviewed

    12 ( 2 )   144 - 147   2006.5

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    To clarify immunoregulatory activity of "Shiranui Himekiku" (Chrysanthemum indicam × Erigeron annus), we examined the effect on tumor necrosis factor α (TNF-α) production by mouse macrophage-like cell line RAW264.7. The dried chrysanthemum flower (CF) petals were extracted with water, and the cells were cultured in the presence of the extract. CF extract significantly enhanced TNF-α production of the cells by the dose-dependent manner. Diluted CF solution did not significantly affect the cell number and viability; however, non-diluted solution suppressed the cell proliferation and decreased the cell viability. Heating CF extract at 100°C for 30 min enhanced the activity of water extract markedly, and freeze-thawing only moderately. The TNF-α production enhancing activity of the extract was observed within 3h. These results suggest that the TNF-α production enhancing activity of CF extract can be recovered efficiently by hot water extraction and preserved stably by freezing.

    DOI: 10.3136/fstr.12.144

  • A lipid raft-associated 67 kDa laminin receptor mediates suppressive effect of epigallocatechin-3-O-gallate on FcεRI expression Reviewed

    336 ( 2 )   674 - 681   2005.10

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    (-)-Epigallocatechin-3-O-gallate (EGCG), a major green tea polyphenol, has previously exhibited a suppressive effect on the expression of the high-affinity IgE receptor (FcεRI). This effect has been shown to be elicited by interaction with the plasma membrane microdomain lipid rafts. Recently, we have identified the 67 kDa laminin receptor (67LR) as a cell surface EGCG receptor that mediates an anti-cancer action. Here we show that the 67LR is highly associated with lipid rafts on human basophilic KU812 cells. Experiments using 67LR-enhanced and -reduced cells revealed that the EGCG&#039;s ability to downregulate FcεRI expression correlated with the amount of 67LR. Thus, these results suggest that the lipid raft-associated 67LR plays an important role in mediating the FcεRI-suppressive action of EGCG. © 2005 Elsevier Inc. All rights reserved.

    DOI: 10.1016/j.bbrc.2005.08.146

  • Negative regulation of the basophil activation by natural ligands for Peroxisome proliferator-activated receptors International journal

    Fujimura, Y., Tachibana, H., and Yamada, K.

    Animal Cell Technology   13   369 - 374   2004.5

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  • A difference between Epigallocatechin-3-gallate and Epicatechin-3-gallate on anti-allergic effect is dependent on their distinct distribution to lipid rafts Reviewed International journal

    Fujimura, Y., Tachibana, H., and Yamada, K.

    Biofactors   21 ( 1-4 )   133 - 135   2004.5

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  • Epigallocatechin gallate associated with cell surface lipid rafts downregulates high affinity IgE receptor through the inhibition of extracellular signal-regulated kinase1/2 phosphorylation Reviewed International journal

    Tachibana, H., Fujimura, Y., and Yamada, K.

    Biofactors   21 ( 1-4 )   2004.5

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  • A receptor for green tea polyphenol EGCG Reviewed

    Hirofumi Tachibana, Kiyoshi Koga, Yoshinori Fujimura, Koji Yamada

    Nature Structural and Molecular Biology   11 ( 4 )   380 - 381   2004.4

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    DOI: 10.1038/nsmb743

  • Lipid raft-associated catechin suppresses the FcεRI expression by inhibiting phosphorylation of the extracellular signal-regulated kinase1/2 Reviewed

    556 ( 1-3 )   204 - 210   2004.1

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    The major green tea catechin, (-)-epigallocatechin-3-O-gallate (EGCG), has a suppressive effect on the expression of the high-affinity IgE receptor FcεRI, which is key molecule in the IgE-mediated allergic reactions. Here we show that EGCG binds to the cell surface and highly associates with plasma membrane microdomains, lipid rafts, on the human basophilic KU812 cells. The disruption of these lipid rafts caused a reduction of the amount of raft-associated EGCG and the FcεRI-suppressive effect of EGCG. We also found that EGCG has an ability to inhibit the phosphorylation of the extracellular signal-regulated kinase1/2 (ERK1/2) and that the ERK1/2 specific inhibitor also reduced FcεRI expression. Moreover, the inhibitory effect elicited by EGCG on ERK1/2 was prevented by disruption of rafts. Thus, these results suggest that the interaction between EGCG and the lipid rafts is important for EGCG&#039;s ability to downregulate FcεRI expression, and ERK1/2 may be involved in this suppression signal. © 2003 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

    DOI: 10.1016/S0014-5793(03)01432-7

  • Downregulation of high affinity IgE receptor FcepsilonRI expression in the human basophilic KU812 cells by a tea catechin International journal

    Fujimura, Y., Tachibana, H., and Yamada, K.

    Animal Cell Technology   12   365 - 370   2002.5

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  • Peroxisome proliferator-activated receptor (PPAR) ligands negatively regulate the expression of the high-affinity IgE receptor FcepsilonRI in human basophilic KU812 cells Reviewed International journal

    Fujimura, Y., Tachibana, H., and Yamada, K.

    Biochem. Biophys. Res. Commun.   297 ( 2 )   193 - 201   2002.5

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    DOI: 10.1016/S0006-291X(02)02139-3

  • Antiallergic tea catechin, (-)-epigallocatechin-3-O-(3-O-methyl)-gallate, suppresses FcepsilonRI expression in human basophilic KU812 cells. Reviewed International journal

    Fujimura, Y., Tachibana, H., Maeda-Yamamoto, M., Miyase, T., Sano, M., and Yamada, K.

    J. Agric. Food Chem.   50 ( 20 )   5729 - 5734   2002.5

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    DOI: 10.1021/jf025680z

  • A tea catechin suppresses the expression of the high-affinity IgE receptor FcepsilonRI in human basophilic KU812 cells Reviewed International journal

    Fujimura, Y., Tachibana, H., and Yamada, K.

    J. Agric. Food Chem.   49 ( 5 )   2527 - 2531   2001.5

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    DOI: 10.1021/jf001392w

  • Antigen binding of an ovomucoid specific antibody is affected by a carbohydrate chain located on the light chain variable region Reviewed International journal

    Fujimura, Y., Tachibana, H., Eto, N., and Yamada, K.

    Biosci. Biotechnol. Biochem.   64 ( 11 )   2298 - 2305   2000.5

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    DOI: 10.1271/bbb.64.2298

  • Modified C-type natriuretic peptide normalizes tumor vasculature, reinvigorates antitumor immunity, and improves solid tumor therapies. International journal

    Zhen Lu, Ioannis Verginadis, Motofumi Kumazoe, Gerardo M Castillo, Yao Yao, Rebecca E Guerra, Sandra Bicher, Menghao You, George McClung, Rong Qiu, Zebin Xiao, Zhen Miao, Subin S George, Daniel P Beiting, Takashi Nojiri, Yasutake Tanaka, Yoshinori Fujimura, Hiroaki Onda, Yui Hatakeyama, Akiko Nishimoto-Ashfield, Katrina Bykova, Wei Guo, Yi Fan, Nikolay M Buynov, J Alan Diehl, Ben Z Stanger, Hirofumi Tachibana, Terence P Gade, Ellen Puré, Constantinos Koumenis, Elijah M Bolotin, Serge Y Fuchs

    Science translational medicine   16 ( 761 )   eadn0904   2024.8   ISSN:1946-6234 eISSN:1946-6242

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    Deficit of oxygen and nutrients in the tumor microenvironment (TME) triggers abnormal angiogenesis that produces dysfunctional and leaky blood vessels, which fail to adequately perfuse tumor tissues. Resulting hypoxia, exacerbation of metabolic disturbances, and generation of an immunosuppressive TME undermine the efficacy of anticancer therapies. Use of carefully scheduled angiogenesis inhibitors has been suggested to overcome these problems and normalize the TME. Here, we propose an alternative agonist-based normalization approach using a derivative of the C-type natriuretic peptide (dCNP). Multiple gene expression signatures in tumor tissues were affected in mice treated with dCNP. In several mouse orthotopic and subcutaneous solid tumor models including colon and pancreatic adenocarcinomas, this well-tolerated agent stimulated formation of highly functional tumor blood vessels to reduce hypoxia. Administration of dCNP also inhibited stromagenesis and remodeling of the extracellular matrix and decreased tumor interstitial fluid pressure. In addition, treatment with dCNP reinvigorated the antitumor immune responses. Administration of dCNP decelerated growth of primary mouse tumors and suppressed their metastases. Moreover, inclusion of dCNP into the chemo-, radio-, or immune-therapeutic regimens increased their efficacy against solid tumors in immunocompetent mice. These results demonstrate the proof of principle for using vasculature normalizing agonists to improve therapies against solid tumors and characterize dCNP as the first in class among such agents.

    DOI: 10.1126/scitranslmed.adn0904

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  • Fustin suppressed melanoma cell growth via cAMP/PKA-dependent mechanism. International journal

    Motofumi Kumazoe, Yoshinori Fujimura, Yu Shimada, Hiroaki Onda, Yui Hatakeyama, Hirofumi Tachibana

    Bioscience, biotechnology, and biochemistry   88 ( 8 )   900 - 907   2024.6   ISSN:0916-8451 eISSN:1347-6947

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    Melanoma, a cancer arising from melanocytes, requires a novel treatment strategy because of the ineffectiveness of conventional therapies in certain patients. Fustin is a flavanonol found in young fustic (Cotinus coggygria). However, little is known about its antimelanoma effects. Our study demonstrates that fustin suppresses the growth of B16 melanoma cells. Phalloidin staining of cytoskeletal actin revealed that fustin induced a conformational change in the actin structure of melanoma cells, accompanied by suppressed phosphorylation of myosin regulatory light chain 2 (MLC2), a regulator of actin structure. Furthermore, the protein kinase A (cAMP-dependent protein kinase) inhibitor H89 completely attenuated fustin-induced downregulation of phosphorylated myosin phosphatase targeting subunit 1, which is involved in dephosphorylation of MLC2. In a mouse model, administration of fustin suppressed tumor growth in B16 melanoma cells without adverse effects. In conclusion, our findings suggest that fustin effectively suppresses melanoma cell growth both in vitro and in vivo.

    DOI: 10.1093/bbb/zbae072

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  • miR-12135 ameliorates liver fibrosis accompanied with downregulation of ITGA11

    Motofumi Kumazoe, Emi Miyamoto, Chihiro Oka, Miyuki Kondo, Ren Yoshitomi, Hiroaki Onda, Yu Shimada, Yoshinori Fujimura, Hirofumi Tachibana

    iScience   27 ( 1 )   108730 - 108730   2024.1   ISSN:2589-0042 eISSN:2589-0042

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Elsevier BV  

    DOI: 10.1016/j.isci.2023.108730

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  • Neuroprotective effect of isovaleraldehyde accompanied with upregulation of BDNF and CREB phosphorylation via the PKA pathway(タイトル和訳中)

    Shimada Yu, Kumazoe Motofumi, Otsuka Yushi, Tetsuzen Rin, Fujimura Yoshinori, Tachibana Hirofumi

    Journal of Natural Medicines   78 ( 1 )   208 - 215   2024.1   ISSN:1340-3443

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  • Neuroprotective effect of isovaleraldehyde accompanied with upregulation of BDNF and CREB phosphorylation via the PKA pathway.

    Yu Shimada, Motofumi Kumazoe, Yushi Otsuka, Rin Tetsuzen, Yoshinori Fujimura, Hirofumi Tachibana

    Journal of natural medicines   78 ( 1 )   208 - 215   2024.1   ISSN:1340-3443 eISSN:1861-0293

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    Recently, the number of patients diagnosed with dementia has increased. The World Health Organization (WHO) estimates that 50 million patients suffer from dementia. Although several therapeutic strategies have been proposed, currently, there is no curative approach for treating dementia. Neurodegeneration is an irreversible process. As this disease gradually progresses over 15-20 years, a low-cost and sustainable method for preventing these diseases is desired. Cacao nib is consumed in many countries, and a recent clinical study indicated that cocoa intake upregulates brain-derived neurotrophic factor (BDNF), which plays a significant role in memory formation and neuronal cell survival. In the present study, neural cells were treated with cacao nib extract or the 17 characteristic components of cacao nib. Treatment with Cacao nib extract upregulates BDNF mRNA expression. In addition, cacao nib extract elicits the phosphorylation of cAMP-response-element-binding protein (CREB), which regulates the transcription of BDNF. Among the 17 species screened, isovaleraldehyde (IVA), also known as an aroma component of cacao nibs extract, improved BDNF mRNA expression without SH-SY5Y cell toxicity. IVA also promoted CREB phosphorylation through a cAMP-dependent protein kinase (PKA)-dependent mechanism. In conclusion, IVA could be responsible for the BDNF upregulation effect of cacao nib, and IVA upregulated BDNF expression via the PKA-CREB axis.

    DOI: 10.1007/s11418-023-01763-1

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  • Effect of theogallin on cognitive function in mice

    Lee, K; Otsuka, Y; Fujimura, Y; Tachibana, H

    ANNALS OF NUTRITION AND METABOLISM   79   1054 - 1054   2023.8   ISSN:0250-6807 eISSN:1421-9697

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  • Delphinidin suppresses disuse muscle atrophy and upregulates microRNA-23a-3p expression in extracellular vesicles derived from intestinal cells

    Marugame, Y; Murata, M; Goto, M; Fujimura, Y; Tachibana, H

    ANNALS OF NUTRITION AND METABOLISM   79   966 - 966   2023.8   ISSN:0250-6807 eISSN:1421-9697

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  • Anti-inflammatory mechanism of EGCG by suppressing expression of Elf-1

    Yamashita, M; Kumazoe, M; Kobarai, H; Nakamura, Y; Fujimura, Y; Tachibana, H

    ANNALS OF NUTRITION AND METABOLISM   79   957 - 957   2023.8   ISSN:0250-6807 eISSN:1421-9697

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  • 抗アレルギー性O-メチル化カテキンに柑橘類フラバノンのヘスペレチンを組み合わせた生物活性促進戦略(Bioactivity-boosting strategy based on combination of anti-allergic O-methylated catechin with a Citrus flavanone, hesperetin)

    Fujimura Yoshinori, Yoshimoto Takanori, Fujino Konatsu, Nezu Ayaka, Marugame Yuki, Bae Jaehoon, Kumazoe Motofumi, Tachibana Hirofumi

    Journal of Natural Medicines   77 ( 2 )   363 - 369   2023.3   ISSN:1340-3443

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    柑橘類フラバノンのヘスペレチンが、ラット好塩基球/肥満細胞株RBL-2H3の抗アレルギー性カテキンの(-)-エピガロカテキン-3-O-(3-O-メチル)ガラート(EGCG3''Me)によるIgE/抗原媒介性脱顆粒の阻害活性を増幅することを見出した。ヘスペレチンは、細胞表面蛋白質の67-kDaラミニン受容体(67LR)を介して、EGCG3''Meの抗アレルギー効果発現に必須の酸性スフィンゴミエリナーゼ(ASM)の活性化を有意に促進した。強く吸収されるα-グルコシルヘスペリジンを経口投与すると、BALB/cマウスにおけるIgE/抗原の受動的皮膚アナフィラキシー(PCA)反応に対するEGCG3''Meの豊富な「べにふうき緑茶」の阻害活性を促進した。ヘスペレチンはASMシグナル伝達の活性化を介して、IgE/抗原媒介性脱顆粒を阻害するEGCG3''Meの活性を増幅することが示された。

  • ケルセチンはヒト子宮頸癌において腫瘍抑制性microRNAの発現を亢進する(Quercetin up-regulates the expression of tumor-suppressive microRNAs in human cervical cancer)

    Murata Motoki, Komatsu Satomi, Miyamoto Emi, Oka Chihiro, Lin Ichian, Kumazoe Motofumi, Yamashita Shuya, Fujimura Yoshinori, Tachibana Hirofumi

    Bioscience of Microbiota, Food and Health   42 ( 1 )   87 - 93   2023.1   ISSN:2186-6953

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    ヒト子宮頸癌細胞中のmiRNAと代表的フラボノイドのケルセチンとの関係を二つの試験で調べた。試験1ではヌードマウスにHeLa細胞を皮下注入して腫瘍が形成された後、マウスを対照群、10mg/kg b.w.ケルセチン群、50mg/kg b.w.ケルセチン群に割り付けた。11日後に子宮頸癌からRNAを抽出した。試験2ではマウスを対照群と50mg/kg b.w.ケルセチン群に割り付け48時間後に血漿からRNAを抽出した。試験1では、ケルセチン投与群の初期の子宮頸癌のmiR-26b、miR-126、miR-320aの発現が亢進された。試験2では、ケルセチンの単回投与は血漿中のmiR-26b、miR-126a、miR-320発現に影響を与えなかった。これらの結果から、ケルセチンは子宮頸癌内の腫瘍抑制miRNA分子の発現を上昇させることが示唆された。HeLa細胞を用いたin vitro試験の結果、ケルセチンは癌細胞に直接作用してmiRNAの亢進を促進することが示された。In vitro試験の結果、ケルセチンはmiR-320aの亢進を介してβ-カテニン濃度を低下させることが判明した。ケルセチンはpre-miR-26bとpre-miR-320aより早い段階でpre-miR-126の発現を増加させることが判明した。

  • Antibody recognition of complement factor H reveals a flexible loop involved in atypical hemolytic uremic syndrome pathogenesis

    Yokoo, T; Tanabe, A; Yoshida, Y; Caaveiro, JMM; Nakakido, M; Ikeda, Y; Fujimura, Y; Matsumoto, M; Entzminger, K; Maruyama, T; Okumura, CJ; Nangaku, M; Tsumoto, K

    JOURNAL OF BIOLOGICAL CHEMISTRY   298 ( 6 )   101962   2022.6   ISSN:0021-9258 eISSN:1083-351X

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  • ゴマリグナンによるグルタチオンS-トランスフェラーゼ活性化作用におけるマイクロRNAの関与の検討

    丸亀 裕貴, 竹下 菜津子, 山田 脩平, 吉富 廉, 熊添 基文, 藤村 由紀, 立花 宏文

    日本栄養・食糧学会大会講演要旨集   76回   286 - 286   2022.5

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  • デルフィニジンの骨格筋線維型変換作用

    高橋 里奈, 村田 希, 丸亀 裕貴, 藤村 由紀, 立花 宏文

    日本栄養・食糧学会大会講演要旨集   76回   260 - 260   2022.5

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  • ゴマリグナンはグルタチオンS-トランスフェラーゼ発現を亢進しmicroRNA-669c-3pを抑制する(Sesame lignans upregulate glutathione S-transferase expression and downregulate microRNA-669c-3p)

    Marugame Yuki, Takeshita Natsuko, Yamada Shuhei, Yoshitomi Ren, Kumazoe Motofumi, Fujimura Yoshinori, Tachibana Hirofumi

    Bioscience of Microbiota, Food and Health   41 ( 2 )   66 - 72   2022.4   ISSN:2186-6953

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    マウスの肝臓中のグルタチオンS-トランスフェラーゼ(GST)とmiRNAの発現に及ぼすゴマリグナンの効果を解明した。セサミン/エピセサミン混合物(SE)を7日間マウスに経口投与した。SE摂取はマウスの体重または肝臓重量を変化させずに肝臓中のGST活性を亢進した。リアルタイムPCRでSE投与後のGSTA1、GSTA4、GSTM4のmRNA発現量は変化しなかったが、GSTA1、GSTA4、GSTM4のタンパク質発現量は増大した。これらの結果から、SEはGSTタンパク質の発現増大によってGST活性を上昇していることが示された。肝臓組織のmiRNAマイクロアレイ解析により、SEは肝臓中の84個のmiRNA発現を亢進し19個のmiRNA発現を抑制することが判明した。miRNA標的予測アルゴリズムにより、SE投与で発現が抑制した19個のmiRNAのうち16個がGSTを標的にしている可能性が示された。定量リアルタイムPCRにより、miR-669c-3pに及ぼすSEの効果が確認された。NMuLi細胞へのmiR-669c-3p mimicのトランスフェクションにより、miR-669c-3pはマウス肝臓中のGSTA4とGSTM4のmRNAとタンパク質の発現を抑制していることが示唆された。

  • Putative protection mechanism of CTL from killing by their own perforin International journal

    Eto, N., Kurokui, S., Ikeda, S., Sone, K., Hirashima, A., Fujimura, Y., Tanino, S., Tachibana, H., Yasuda, M., and Liu, C.C.

    Animal Cell Technology   12   2002.5

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Books

  • Genomics, Proteomics and Metabolomics in Nutraceuticals and Functional Foods, 2nd Edition

    Fujimura Y, Tachibana H( Role: Joint author)

    Wiley-Blackwell  2015.9 

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    Language:English   Book type:Scholarly book

  • Handbook of Green Tea and Health Research

    Fujimura, Y. and Tachibana, H.( Role: Joint author)

    Nova Science Publishers Inc.  2009.5 

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    Language:English   Book type:Scholarly book

  • 67‐kDa Laminin Receptor‐Mediated Cellular Sensing System of Green Tea Polyphenol EGCG and Functional Food Pairing

    藤村 由紀

    Molecules  2022 

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    Total pages:1  

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  • Genomics, Proteomics and Metabolomics in Nutraceuticals and Functional Foods, 2nd Edition (Chapter 31: Metabolomics of Green Tea)

    Fujimura Y, Tachibana H( Role: Joint author)

    Wiley-Blackwell  2015.4 

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  • 食物アレルギーの現状とリスク低減化食品素材の開発(マスト細胞・好塩基球を用いた脱顆粒抑制試験)

    立花 宏文, 藤村 由紀( Role: Joint author)

    シーエムシー出版  2015.3 

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  • 新版 茶の機能(最近の成分分析法 「メタボリック・プロファイリング」)

    藤村 由紀, 三浦 大典, 割石 博之, 立花 宏文( Role: Joint author)

    農山漁村文化協会  2013.10 

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  • Handbook of Green Tea and Health Research (Molecular basis for anti-cancer activity of EGCG in vivo: Molecular-targeting prevention of cancer by green tea catechin)

    Fujimura Y, Tachibana H( Role: Joint author)

    Nova Science Publishers Inc  2009.5 

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  • 茶の効能と応用開発(茶の生理活性・抗アレルギー作用)

    藤村 由紀, 山本(前田)万里, 立花 宏文( Role: Joint author)

    2006.5 

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Presentations

  • 機能性フードペアリングに向けたメタボリック・プロファイリング解析 Invited

    藤村 由紀, 熊添 基文, 立花 宏文

    第77回日本栄養・食糧学会大会  2023.5 

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    Event date: 2023.5

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:札幌市   Country:Japan  

  • Metabolic profiling for the bioactivity evaluation of green tea and the selection of bioactivity-related chemical combinations. Invited International conference

    Fujimura Y, Miura D, and Tachibana H.

    The 9th International Conference on Polyphenols and Health (ICPH2019)  2019.11 

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    Event date: 2019.11 - 2019.12

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  • 機能性フードペアリングのABC Invited

    藤村 由紀, 三浦 大典, 立花 宏文

    JASISコンファレンス ライフサイエンスイノベーションフォーラムI  2019.9 

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    Event date: 2019.9

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:幕張市   Country:Japan  

  • MALDI-MSイメージング・プロファイリング法が切り開くフードミクスの新展開 Invited

    三浦 大典, 藤村 由紀, 立花 宏文

    日本プロテオーム学会2019年度大会/第70回日本電気泳動学会総会(JPrOS2019/JES2019)  2019.7 

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    Event date: 2019.7

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:宮崎市   Country:Japan  

  • 緑茶の機能性を多面的に捉えるメタボリック・プロファイリング Invited

    藤村由紀, 三浦大典, 立花宏文

    日本農芸化学会2019年大会  2019.3 

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    Event date: 2019.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:東京都   Country:Japan  

  • 食品の機能性を読み解く質量分析を基盤とするプロファイリング術 Invited

    藤村由紀

    第428回 福岡県保健環境研究所集談会  2019.2 

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    Event date: 2019.2

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:太宰府市   Country:Japan  

  • Metabolic Profiling Strategy for the Bioactivity Evaluation of Green Tea and the Selection of Bioactivity-related Chemical Combinations Invited International conference

    Fujimura Y, Miura D, Tachibana H

    The 3rd Symposium of Kyoto Biomolecular Mass Spectrometry Society  2019.2 

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    Event date: 2019.2

    Language:English   Presentation type:Oral presentation (general)  

    Country:Japan  

  • 食品の機能性と安全性の科学的根拠を読み解くプロファイリング術 Invited

    藤村由紀

    第17回石川県立大学食品科学科公開セミナー  2018.11 

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    Event date: 2018.11

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:野々市   Country:Japan  

  • 質量分析イメージング技術の開発と食品科学分野への応用 Invited

    藤村由紀

    第一回プロテオミクス先端技術研究会  2018.10 

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    Event date: 2018.10

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:宮崎市   Country:Japan  

  • 高解像度の表現型解析に向けた質量分析によるメタボリック・プロファイリング Invited

    藤村由紀

    九州大学農学研究院 研究教育支援センター主催 質量分析・新技術セミナー  2017.7 

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    Event date: 2018.7

    Language:Japanese   Presentation type:Public lecture, seminar, tutorial, course, or other speech  

    Venue:福岡市   Country:Japan  

  • 機能性食品の科学的根拠を読み解くプロファイリング術 Invited

    藤村由紀

    日本食品安全協会・健康食品管理士養成セミナー  2018.6 

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    Event date: 2018.6

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:福岡市   Country:Japan  

  • 生体レドックスに及ぼす緑茶ポリフェノールの影響 Invited

    藤村由紀, 兵藤文紀, 立花宏文

    2018年度(第22回)生物機能研究会  2018.6 

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    Event date: 2018.6

    Language:Japanese  

    Venue:福岡市   Country:Japan  

  • ニュートリメタボロミクスを切り拓く複合成分系食品の機能性プロファイリング Invited

    藤村由紀, 立花宏文

    日本農芸化学会西日本支部第322回支部例会  2018.5 

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    Event date: 2018.5

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:福岡市   Country:Japan  

  • 複合成分系である食品の機能性を高精度に捉えるニュートリメタボロミクス Invited

    藤村由紀, 三浦大典, 立花宏文

    第39回日本臨床栄養学会総会・第38回日本臨床栄養協会総会 第15回大連合大会  2017.10 

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    Event date: 2017.10

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:千葉市   Country:Japan  

  • メタボロミクス技術を食品の機能性研究にどうやって活かすか? Invited

    藤村 由紀

    宮崎大学大学院農学工学総合研究科 食の科学ユニット講演会  2016.11 

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    Event date: 2016.11

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:宮崎市   Country:Japan  

  • 質量分析メージングによる高精度病態評価および生体応答解析 Invited

    藤村 由紀

    KRIワークショップ  2016.10 

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    Event date: 2016.10

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:京都市   Country:Japan  

  • 緑茶カテキンのシグナリングとイメージングの基礎 Invited

    藤村 由紀

    第9回トランスポーター研究会九州部会  2016.10 

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    Event date: 2016.10

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:宮崎市   Country:Japan  

  • 食品の特性を可視化する質量分析イメージングによるニュートリメトリクス Invited

    藤村 由紀

    第12回レドックス・ライフイノベーションシンポジウム  2016.8 

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    Event date: 2016.8

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:熊本市   Country:Japan  

  • メタボロミクスが切り拓く食品分析の新たなソリューション Invited

    藤村 由紀

    第111回日本食品衛生学会学術講演会  2016.5 

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    Event date: 2016.5

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Venue:東京都   Country:Japan  

  • 要素還元的手法の限界とメタボロミクスの挑戦:食品機能性評価への応用 Invited

    藤村 由紀

    日本農芸化学会2016年度大会  2016.3 

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    Event date: 2016.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:札幌市   Country:Japan  

  • 代謝プロファイリングおよびイメージングを可能とするMALDI-MS技術の開発 Invited

    藤村 由紀

    第20回異物・異臭に関する勉強会  2016.3 

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    Event date: 2016.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:蕨市   Country:Japan  

  • 先端メタボロミクスによる新たな食品分析ソリューション Invited

    藤村 由紀

    九大発 産・学・官 交流促進シーズ発表会  2016.2 

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    Event date: 2016.2

    Language:Japanese  

    Venue:福岡市   Country:Japan  

  • 胎動、フードケミカルバイオロジーが切開く機能性食品成分の新たな生体応答機構 Invited

    藤村由紀

    愛媛大学農学部セミナー  2009.6 

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    Event date: 2009.6

    Language:Others   Presentation type:Oral presentation (general)  

    Venue:松山市   Country:Japan  

  • 農学研究者のためのメタボロミクス “サルでもわかるメタボロミクス” Invited

    藤村由紀

    九州大学バイオアーキテクチャーセンターセミナー  2008.11 

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    Event date: 2008.11

    Language:Others   Presentation type:Oral presentation (general)  

    Venue:福岡市   Country:Japan  

  • 植物ポリフェノールの抗アレルギー作用に関する研究 Invited

    矢野知美, 梅田大介, 藤村由紀, 山田耕路, 立花宏文

    生物機能研究会講演会  2008.6 

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    Event date: 2008.6

    Language:Others   Presentation type:Oral presentation (general)  

    Venue:福岡市   Country:Japan  

  • Green tea polyphenol EGCG signaling through 67 kDa laminin receptor Invited International conference

    Tachibana, H., Daisuke, U., Fujimura, Y., and Yamada, K.

    The 4th International Conference on Food Factors  2007.11 

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    Event date: 2007.11 - 2007.12

    Language:Others   Presentation type:Symposium, workshop panel (public)  

    Country:Japan  

  • Prevention of allergy by dietary polyphenols Invited International conference

    Tachibana, H, Fujimura, Y., Yano, S., Umeda, D., and Yamada, K.

    13th International Congress of Mucosal Immunology  2007.7 

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    Event date: 2007.7

    Language:Others   Presentation type:Symposium, workshop panel (public)  

    Country:Japan  

  • 健康を科学する食のトリビア?

    藤村 由紀

    純真女子短期大学公開講座  2004.9 

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    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Other  

  • お茶に秘められた健康増進パワー!

    藤村 由紀

    純真女子短期大学公開講座  2004.9 

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  • 最近の機能性食品素材の研究・開発動向

    藤村 由紀

    (社)福岡県栄養士協会福岡支部 [南分会]  2005.7 

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  • 機能性食品成分の標的受容体分子制御に基づく抗アレルギーシグナルの解明 Invited

    藤村 由紀

    日本農芸化学会西日本支部総会  2006.1 

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  • 緑茶カテキンの抗アレルギー作用を解き明かす新たなシグナルプラットフォーム Invited

    藤村 由紀

    生物機能研究会講演会  2006.6 

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  • 先端融合医療レドックスナビ研究拠点におけるメタボリック・プロファイリング研究 Invited

    藤村 由紀

    生物機能研究会講演会  2008.6 

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  • ここまでわかったお茶の健康パワー Invited

    藤村 由紀

    身の回りの毒に強くなる会  2008.10 

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  • “サルでもわかるメタボロミクス”

    藤村 由紀

    九州大学バイオアーキテクチャーセンターセミナー  2008.11 

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  • お茶の機能性最前線

    藤村 由紀

    快適環境創造の会  2008.11 

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  • フードケミカルバイオロジーが切開く機能性食品成分の新たな生体応答機構 Invited

    藤村 由紀

    愛媛大学農学部セミナー  2009.6 

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  • 機能性食品成分の生体応答の分子機構 Invited

    藤村 由紀

    愛媛大学農学部セミナー  2009.6 

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  • 社会の明るい未来を切り開くプロファイリング研究

    藤村 由紀

    フードサイエンスフォーラム  2009.9 

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  • Molecular basis for anti-inflammatory actions of dietary polyphenols Invited International conference

    Japan Society for Bioscience, Biotechnology, and Agrochemistry  2010.3 

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  • お茶の健康パワー Invited

    藤村 由紀

    いとしまサイエンスキャラバン2010  2011.3 

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  • ニュートリメタボロミクスを切り拓く多元的質量分析システムの開発 Invited

    藤村 由紀

    平成23年度日本農芸化学会西日本支部若手シンポジウム  2011.9 

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  • 食品機能性評価に向けた代謝物プロファイリング技術の開発

    藤村 由紀

    産学官連携技術シーズセミナー in 福岡  2011.9 

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  • レドックス関連疾患の理解とニュートリメタボロミクス Invited

    藤村 由紀

    第65回日本酸化ストレス学会学術集会  2012.6 

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  • 質量分析を基盤としたプロテオミクス技術のメタボローム研究への応用 Invited

    藤村 由紀

    第36回蛋白質と酵素の構造と機能に関する九州シンポジウム  2012.9 

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  • 食品機能性評価に向けたニュートリメタボロミクス Invited

    藤村 由紀

    第7回メタボロームシンポジウム  2012.10 

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  • MALDI質量分析法を基盤としたメタボロミクス技術の表現型解析への応用 Invited

    藤村 由紀

    平成24年度神戸大学大学院医学研究科先端医学シリーズ(GCOEプログラム)「画像解析・イメージング、病態診断、メタボローム研究の最先端」  2012.12 

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  • 組織内微小領域における機能性食品因子の時空間分解可視化 Invited

    藤村 由紀

    日本農芸化学会2013年度大会  2013.3 

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  • 低分子食品成分の組織内微小空間分布の非標識可視化法 Invited

    藤村 由紀

    第67回日本栄養・食糧学会大会  2013.5 

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  • 緑茶の機能性を捉える低分子ケミカルセンシングに関する研究 Invited

    藤村 由紀

    日本農芸化学会2014年度大会  2014.3 

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  • The biological function of tea via EGCG receptor Invited International conference

    The 2014 Annual Meeting and Symposium of Health Food Science of Taiwan  2014.3 

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  • Metabolomics-driven chemical sensing strategy for better understanding of functions of green tea Invited International conference

    2014 Water-Algae-Tea Consortium for Health (WATCH)  2014.3 

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  • 緑茶の機能性を捉える低分子ケミカルセンシングに関する研究 Invited

    藤村 由紀

    第306回日本農芸化学会西日本支部例会  2014.5 

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  • MALDI-MSを基盤とした代謝プロファイリング技術の開発 Invited

    藤村 由紀

    第27回九州分析化学会若手の会 春の講演会  2014.5 

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  • 食品機能性評価に向けたメタボロミクスの技術開発に関する研究 Invited

    藤村 由紀

    生物機能研究会講演会  2014.7 

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  • 食品の機能性を捉える低分子ケミカルセンシング法の開発 Invited

    藤村 由紀

    サントリー研究センター講演会  2014.11 

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  • 要素還元的手法を補完するメタボロミクス:緑茶の機能性評価への応用 Invited

    藤村 由紀

    日本食品科学工学会第62回大会  2015.8 

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  • 要素還元的手法を補完するメタボロミクスの食品分析への応用

    藤村 由紀

    バイオインダストリー協会セミナー“未来へのバイオ技術”勉強会  2015.12 

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  • 緑茶カテキンの生体組織内分布の新たな可視化技術 Invited

    藤村 由紀

    第12回 日本カテキン学会年次学術集会  2015.12 

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  • 肝臓特異的67-kDa laminin receptorノックアウトマウスを用いた胆汁酸による肝線維化促進メカニズムの解明

    磯貝 航, 米山 拓良, 一瀬 智美, 竹下 菜津子, 錦戸 里紗, 熊添 基文, 藤村 由紀, 立花 宏文

    日本栄養・食糧学会大会講演要旨集  2024.4  (公社)日本栄養・食糧学会

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  • 緑茶摂取前後のヒト血液中に存在する緑茶由来miRNAの変動解析

    渡邉 楓, 渡邉 凌矢, 山本 真生, 熊添 基文, 藤村 由紀, 立花 宏文

    日本栄養・食糧学会大会講演要旨集  2024.4  (公社)日本栄養・食糧学会

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  • 緑茶成分ストリクチニンの短期投与による老齢および若齢マウスの認知機能調節作用

    松井 優樹, Lee Kwanwoo, 熊添 基文, 藤村 由紀, 立花 宏文

    日本栄養・食糧学会大会講演要旨集  2023.3  (公社)日本栄養・食糧学会

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  • 緑茶カテキン代謝物EGC-M5による免疫チェックポイント阻害剤の抗腫瘍効果増強作用

    仲嶋 美里, 川本 礼乃, 冨岡 玲乃, 田中 裕子, 熊添 基文, 藤村 由紀, 立花 宏文

    日本栄養・食糧学会大会講演要旨集  2024.4  (公社)日本栄養・食糧学会

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  • 緑茶カテキンEGCG誘導性サーキュラーRNAの機能性解析

    吉富 廉, 藤村 由紀, 立花 宏文

    日本栄養・食糧学会大会講演要旨集  2022.5  (公社)日本栄養・食糧学会

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  • 緑茶カテキンEGCGの骨髄性細胞67LRを介した肝線維化抑制作用

    後藤 芽衣, 麻生 菜帆, 山本 真央, 丸亀 裕貴, 熊添 基文, 藤村 由紀, 立花 宏文

    日本栄養・食糧学会大会講演要旨集  2024.4  (公社)日本栄養・食糧学会

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  • 緑茶カテキンEGCGの急性肺障害抑制作用とそのメカニズム

    高木 啓吾, 小原井 春香, 西岡 成汰, 畠山 結, 熊添 基文, 藤村 由紀, 立花 宏文

    日本栄養・食糧学会大会講演要旨集  2024.4  (公社)日本栄養・食糧学会

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  • 緑茶カテキンEGCGの67LR依存的な抗肥満作用における腸内細菌叢解析

    麻生 菜帆, 西岡 成汰, 小原井 春香, 冨岡 玲乃, 村田 京介, 熊添 基文, 藤村 由紀, 立花 宏文

    日本栄養・食糧学会大会講演要旨集  2022.5  (公社)日本栄養・食糧学会

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  • 抹茶の新たな機能性食品因子としてのマイクロRNAの抽出法の検証

    黄 苡嵐, 一瀬 智美, 渡邉 凌矢, 辰己 由華, 一谷 正己, 衣笠 仁, 瀧川 孝宣, 熊添 基文, 立花 宏文, 藤村 由紀

    日本栄養・食糧学会大会講演要旨集  2024.4  (公社)日本栄養・食糧学会

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  • メタボリック・プロファイリング法による緑茶の抗糖尿病活性に資する成分・パターンの解析

    一瀬 智美, 渡邉 喬嗣, 山本 真生, 熊添 基文, 藤村 由紀, 立花 宏文

    日本栄養・食糧学会大会講演要旨集  2023.3  (公社)日本栄養・食糧学会

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  • マウス海馬におけるアミロイドβ誘導性神経毒性に対するペンタガロイルグルコース経口摂取の保護作用

    高木 基光, 李 寛雨, 松井 優樹, 島田 優, 熊添 基文, 藤村 由紀, 立花 宏文

    日本栄養・食糧学会大会講演要旨集  2023.3  (公社)日本栄養・食糧学会

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  • ペンタガロイルグルコースの老齢マウス海馬における神経新生促進作用

    李 寛雨, 松井 優樹, 藤村 由紀, 立花 宏文

    日本栄養・食糧学会大会講演要旨集  2022.5  (公社)日本栄養・食糧学会

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  • ペンタガロイルグルコースのマイクロRNA miR-191-5pを介した脳機能調節作用

    岸 洸聖, 李 寛雨, 麻生 菜帆, 松井 優樹, 高木 基光, 藤村 由紀, 立花 宏文

    日本栄養・食糧学会大会講演要旨集  2024.4  (公社)日本栄養・食糧学会

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  • プロシアニジンC1のマイクロRNAmiR-181-5pを介した脳機能調節作用

    千葉 涼太郎, 梅本 紗希, 李 寛雨, 熊添 基文, 藤村 由紀, 立花 宏文

    日本栄養・食糧学会大会講演要旨集  2024.4  (公社)日本栄養・食糧学会

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  • プロシアニジンC1のマイクロmiR-181a-5pを介した骨格筋の分化促進作用

    右橋 陸, 丸亀 裕貴, 梅本 紗希, 熊添 基文, 藤村 由紀, 立花 宏文

    日本栄養・食糧学会大会講演要旨集  2024.4  (公社)日本栄養・食糧学会

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  • ファイトケミカル研究の最前線 緑茶カテキンの多彩な機能性を紐解く生体のセンシング機構の解明

    藤村 由紀, 熊添 基文, 立花 宏文

    日本栄養・食糧学会大会講演要旨集  2024.4  (公社)日本栄養・食糧学会

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  • ヒト血漿中に存在する植物miRNAとその機能解析

    野内 綾太, 近藤 美裕貴, 熊添 基文, 藤村 由紀, 立花 宏文

    日本栄養・食糧学会大会講演要旨集  2024.4  (公社)日本栄養・食糧学会

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  • パーキンソン病緩和作用を示す植物マイクロRNAの同定

    島田 優, 熊添 基文, 恩田 弘明, 小川 史代, 藤村 由紀, 立花 宏文

    日本栄養・食糧学会大会講演要旨集  2024.4  (公社)日本栄養・食糧学会

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  • デルフィニジンセンシング遺伝子の同定とその機能解析

    村田 希, 丸亀 裕貴, 熊添 基文, 藤村 由紀, 立花 宏文

    日本栄養・食糧学会大会講演要旨集  2023.3  (公社)日本栄養・食糧学会

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  • デルフィニジンにより放出が誘導されたマイクロRNAの機能解析

    村田 希, 竹内 陽奈子, 丸亀 裕貴, 藤村 由紀, 立花 宏文

    日本栄養・食糧学会大会講演要旨集  2024.4  (公社)日本栄養・食糧学会

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  • カカオ由来香気成分のBDNF発現促進作用

    島田 優, 林 哲全, 大塚 悠史, 熊添 基文, 藤村 由紀, 立花 宏文

    日本栄養・食糧学会大会講演要旨集  2022.5  (公社)日本栄養・食糧学会

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  • 「機能性フードペアリング:食品因子間の機能的な相互作用」 機能性フードペアリングに向けたメタボリック・プロファイリング解析

    藤村 由紀, 熊添 基文, 立花 宏文

    日本栄養・食糧学会大会講演要旨集  2023.3  (公社)日本栄養・食糧学会

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MISC

  • 67-kDa Laminin Receptor-Mediated Cellular Sensing System of Green Tea Polyphenol EGCG and Functional Food Pairing. Reviewed

    Yoshinori Fujimura, Motofumi Kumazoe, Hirofumi Tachibana

    Molecules (Basel, Switzerland)   2022.8

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    DOI: 10.3390/molecules27165130

  • メタボローム解析を活用した機能性フードデザイン

    藤村 由紀, 三浦 大典, 立花 宏文

    食品分野におけるメタボリック・プロファイリング   2021.6

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  • メタボロミクス技術が切り拓く食機能デザイン研究

    藤村 由紀, @三浦 大典, 立花 宏文

    ソフト・ドリンク技術資料   2021.6

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  • 食機能シグナル発動因子を捉えるセンシング技術

    藤村 由紀, 三浦 大典, 立花 宏文

    実験医学増刊号「食と健康を結ぶメディカルサイエンス」, 38(10), 203-208 (1773-1778),   2020.6

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  • 緑茶成分のメタボリック・プロファイリング解析

    藤村由紀, 立花宏文

    茶ポリフェノールの生理機能と応用展開, 17-23,   2019.9

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  • 緑茶カテキンセンシングと機能性増強技術

    藤村 由紀, 立花 宏文

    機能性食品と薬理栄養「緑茶の機能性と薬理」, 13, 16-21,   2019.8

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  • 緑茶ポリフェノールの機能性を切り開くメタボリック・プロファイリング技術.

    藤村由紀, 立花宏文

    ケミカルエンジニヤリング, 63, 816-821,   2018.11

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  • 抗炎症・抗アレルギー作用

    藤村 由紀, 林 宜蒨, 吉本 孝憲, 立花 宏文

    機能性食品開発のための初期評価試験プロトコール集, 188-207,   2017.9

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  • 質量分析イメージング技術による緑茶ポリフェノールの組織内分布のラベルフリー可視化戦略

    藤村由紀, 三浦大典, 立花宏文

    オレオサイエンス,   2017.6

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  • 質量分析イメージングによる高精度病態評価および生体応答解析

    藤村 由紀

    KRIニュースレター, 55, 10,   2017.4

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  • メタボロミクスが切り拓く食品分析の新たなソリューション

    藤村 由紀

    食品衛生学雑誌, 57, J-148-151,   2016.10

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  • カテキンシグナリングならびにイメージングの基礎

    藤村 由紀, 立花 宏文

    化学と生物, 54, 674-680,   2016.8

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  • マスト細胞・好塩基球を用いた脱顆粒抑制試験

    立花 宏文, 藤村 由紀

    食物アレルギーの現状とリスク低減化食品素材の開発, 144-148,   2015.3

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  • 最近の成分分析法 「メタボリック・プロファイリング」 新版 茶の機能

    藤村 由紀, 三浦 大典, 割石 博之, 立花 宏文

    2013.10

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  • Molecular basis for anti-cancer activity of EGCG in vivo: Molecular-targeting prevention of cancer by green tea catechin

    Fujimura, Y. and Tachibana, H.

    2009.5

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  • 茶の生理活性・抗アレルギー作用

    藤村由紀, 山本(前田)万里, 立花宏文

    2006.5

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  • 緑茶ポリフェノールの抗アレルギー作用: ヒト好塩基球における高親和性IgE受容体発現の抑制

    立花宏文, 藤村由紀, 山田耕路

    食品工業   2002.5

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  • 「機能性フードペアリング」に向けた緑茶のメタボリック・プロファイリング

    藤村 由紀, 立花 宏文

    バイオサイエンスとインダストリー   81 ( 6 )   501 - 504   2023.11   ISSN:0914-8981

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  • 緑茶カテキンEGCGの骨髄性細胞67LRを介したNASH抑制作用

    後藤芽衣, 麻生菜帆, 小原春香, 山本真生, 丸亀裕貴, 熊添基文, 藤村由紀, 立花宏文

    日本フードファクター学会学術集会講演要旨集   28th (CD-ROM)   2023

  • マイクロRNAを介したウーロンホモビスフラバンBの脂質代謝関連遺伝子調節作用

    丸亀裕貴, 吉富廉, 楊琳云, 藤村由紀, 立花宏文

    日本家政学会九州支部大会研究発表要旨集   67th   2023

  • デルフィニジンセンシング遺伝子の同定とその機能解析

    村田希, 丸亀裕貴, 熊添基文, 藤村由紀, 立花宏文

    日本栄養・食糧学会大会講演要旨集   77th   2023

  • デルフィニジンの筋線維型変換作用とそのメカニズム

    高橋里奈, 村田希, 丸亀裕貴, 藤村由紀, 立花宏文

    日本農芸化学会西日本支部大会およびシンポジウム講演要旨集   2023 (CD-ROM)   2023

  • 緑茶カテキンEGCG誘導性サーキュラーRNAの機能性解析

    吉富廉, 藤村由紀, 立花宏文

    日本栄養・食糧学会大会講演要旨集   76th   2022

  • The effects of green tea polyphenol EGCG are mediated by extracellular vesicles derived from hepatocytes

    宮脇早希, 村田希, 村田希, 丸亀裕貴, 藤村由紀, 立花宏文

    日本農芸化学会大会講演要旨集(Web)   2022   2022   ISSN:2186-7976

  • Delphinidin exerts anti-inflammatory effects through extracellular vesicles

    竹内陽奈子, 村田希, 村田希, 丸亀裕貴, 藤村由紀, 立花宏文

    日本農芸化学会大会講演要旨集(Web)   2022   2022   ISSN:2186-7976

  • 脳機能関連遺伝子発現に与えるテオガリン経口投与の影響

    李 寛雨, 大塚 悠史, 藤村 由紀, 米倉 円佳, 嶋本 泰代, 立花 宏文

    日本栄養・食糧学会大会講演要旨集   2021.7

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  • 緑茶カテキンEGCGのマウス肝臓におけるサーキュラーRNA発現調節作用

    吉富 廉, 藤村 由紀, 立花 宏文

    日本栄養・食糧学会大会講演要旨集   2021.7

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  • ウーロンホモビスフラバンBの肝臓脂質代謝関連遺伝子発現調節作用におけるマイクロRNAの関与

    河田 奈摘, 楊 琳云, 丸亀 裕貴, 吉富 廉, 藤村 由紀, 立花 宏文

    日本栄養・食糧学会大会講演要旨集   2021.7

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  • 緑茶カテキンEGCGはマクロファージの67-kDa laminin recoptorを介して抗肥満作用を発揮する

    西岡 成汰, 村田 京介, 冨岡 玲乃, 福富 拓哉, 山田 脩平, 熊添 基文, 藤村 由紀, 立花 宏文

    日本栄養・食糧学会大会講演要旨集   2020.4

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  • 腸管上皮細胞由来の細胞外小胞におけるDelphinidinの筋萎縮予防miR-23aの発現上昇作用

    丸亀 裕貴, 村田 希, 後藤 萌, 藤村 由紀, 立花 宏文

    日本栄養・食糧学会大会講演要旨集   2020.4

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  • 緑茶摂取によるヒト末梢血細胞におけるエピジェネティック制御

    山本 真生, 廣井 舜, 熊添 基文, 藤村 由紀, 山本 万里[前田], 花房 俊昭, 立花 宏文

    日本栄養・食糧学会大会講演要旨集   2020.4

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  • 緑茶カテキンEGCGの筋萎縮抑制作用メカニズム解明ならびにエリオジクチオールによる増強

    村田 希, 丸亀 裕貴, 清水 友貴, 熊添 基文, 藤村 由紀, 立花 宏文

    日本栄養・食糧学会大会講演要旨集   2020.4

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  • オミクスソリューションが切り開くアグリバイオ研究の新たなビジョン MALDI-MSプロファイリング・イメージング法が切り開くフードミクスの新展開

    三浦 大典, 藤村 由紀, 立花 宏文

    電気泳動   2019.7

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  • ウーロンホモビスフラバンBセンシングにおける緑茶カテキン受容体の関与

    Bae Jaehoon, 熊添 基文, 藤村 由紀, 立花 宏文

    日本栄養・食糧学会大会講演要旨集   2019.4

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  • 抗体産生誘導を利用した緑茶カテキンEGCGの67LRアゴニスト作用評価

    大塚 悠史, 日高 詩織, 竹内 智枝理, 熊添 基文, 藤村 由紀, 立花 宏文

    日本栄養・食糧学会大会講演要旨集   2019.4

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  • 分泌型葉酸受容体Folate receptor 3によるホモシステインの神経細胞毒性低減化

    吉富 廉, 中山 魁, 藤村 由紀, 立花 宏文

    日本栄養・食糧学会大会講演要旨集   2019.4

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  • べにふうき緑茶とエリオジクチオール配糖体の機能性フードペアリング

    藤野 小夏, 吉本 孝憲, 篠田 有希, 岡田 賢次, 藤村 由紀, 立花 宏文

    日本栄養・食糧学会大会講演要旨集   2018.4

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  • 食に関連するバイオマーカー 最新の動向 複合成分系である食品の機能性を高精度に捉えるニュートリメタボロミクス

    藤村 由紀, 三浦 大典, 立花 宏文

    2017.9

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  • MALDI-MS代謝物プロファイリング法の開発と緑茶の抗酸化活性評価への応用

    藤村 由紀, 河野 ちひろ, 立花 宏文, 割石 博之, 三浦 大典

    日本栄養・食糧学会大会講演要旨集   2017.4

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  • 植物における質量分析イメージング法の最適化

    一瀬智美, 早川英介, 中村純也, 中家修一, 山崎雄三, 藤村由紀, 割石博之, 三浦大典

    日本農芸化学会大会講演要旨集(Web)   2016.3

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  • 質量分析イメージング法を用いたトマト果実の生理応答に対する代謝動態解析

    中村純也, 一瀬智美, 早川英介, 藤村由紀, 割石博之, 割石博之, 三浦大典

    日本農芸化学会大会講演要旨集(Web)   2016.3

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  • 要素還元的手法の限界とメタボロミクスの挑戦:食品機能性評価への応用

    藤村由紀, 三浦大典, 割石博之, 割石博之, 立花宏文, 立花宏文

    日本農芸化学会大会講演要旨集(Web)   2016.3

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  • 緑茶ポリフェノールの生体内代謝の二次元可視化

    藤村 由紀, 三浦 大典, 割石 博之, 立花 宏文

    ビタミン   2016.1

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    DOI: 10.20632/vso.90.1_26

  • MALDI-MSイメージング法の食品機能性研究への応用:生理活性ポリフェノールの生体内代謝の二次元可視化

    藤村 由紀, 立花 宏文, 割石 博之, 三浦 大典

    2015.12

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    DOI: 10.5702/massspec.S15-28

  • 過塩素酸可溶性蛋白質の転写調節機構の解析

    中村 拓也, 松尾 哲孝, 藤村 由紀, 藤田 秋一, 叶内 宏明

    生物機能研究   2015.10

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  • Metabolomics of Green Tea

    Yoshinori Fujimura, Hirofumi Tachibana

    Genomics, Proteomics and Metabolomics in Nutraceuticals and Functional Foods: Second Edition   2015.10

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    DOI: 10.1002/9781118930458.ch31

  • 1P-126 MS/MSスペクトルネットワークと化合物データベースによる代謝物構造決定法の開発(オミクス解析,一般講演)

    早川 英介, 藤村 由紀, 三浦 大典

    日本生物工学会大会講演要旨集   2015.9

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  • MALDI mass spectrometry imagingによる凍結組織アレイ包埋乳がん組織の低分子代謝産物プロファイリング

    寅田信博, 久保真, 三浦大典, 大内田研宙, 宮崎哲之, 藤村由紀, 早川英介, 大塚隆生, 宮坂義浩, 真鍋達也, 小田義直, 水元一博, 中村雅史

    2015.8

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  • 要素還元的手法を補完するメタボロミクス:緑茶の機能性評価への応用

    藤村由紀, 三浦大典, 割石博之, 立花宏文

    日本食品科学工学会大会講演集   2015.8

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  • Sensomicsによるコーヒーの品質を決定する原料生豆中の成分指標の発見

    岩佐恵子, 瀬戸山大樹, 清水広朗, 瀬田玄通, 藤村由紀, 三浦大典, 割石博之, NAGAI Chifumi, 中原光一

    日本食品科学工学会大会講演集   2015.8

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  • MALDIマトリックスの構造機能相関と戦略的合成展開

    三浦大典, 石井孝典, 早川英介, 藤村由紀, 割石博之, 新藤充

    質量分析総合討論会講演要旨集   2015.6

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  • イメージング質量顕微鏡によるファイトケミカルの高感度分布解析

    藤村由紀, 中村純也, 一瀬智美, KIM Yoonhee, 佐々木雅子, 海野結実, 緒方是嗣, 中島宏樹, 立花宏文, 割石博之, 三浦大典

    日本農芸化学会大会講演要旨集(Web)   2015.3

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  • 緑茶の機能性を捉える低分子ケミカルセンシング

    藤村 由紀, 三浦 大典, 割石 博之, 山田 耕路, 立花 宏文

    生物機能研究会誌   2014.10

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  • NAFLDモデルマウスをサンプルとした脂質解析

    三浦 大典, 藤村 由紀, 海野 結実, 山口 亮, 緒方 是嗣

    島津テクニカルレポート   2014.8

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  • MALDI-MSイメージングによる緑茶カテキンの動物組織内分布解析

    藤村 由紀, 三浦 大典, 立花 宏文

    2014.7

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  • Analysis of Lipids in a NAFLD Model Mouse,SHIMADZU Technical Reports

    Miura D, Fujimura Y, Unno Y, Yamaguchi R, Ogata K

    SHIMADZU Technical Reports   2014.4

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  • メトホルミンとリラグルチドはPKC‐NAD(P)Hオキシダーゼの経路を介して酸化ストレスを減少させる

    BATCHULUUN Battsetseg, 井口登與志, 園田紀之, 佐々木修二, 井上智彰, 藤村由紀, 三浦大典, 高柳涼一

    日本内分泌学会雑誌   2014.4

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  • メトホルミンおよびリラグルチドはPKC‐NAD(P)Hオキシダーゼ系の抑制により高血糖誘導酸化ストレスを抑制する

    BATCHULUUN Battsetseg, 井口登與志, 園田紀之, 佐々木修二, 井上智彰, 藤村由紀, 三浦大典, 高柳涼一

    糖尿病   2014.4

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  • OP-062-1 凍結組織アレイおよび質量分析イメージングをもちいた乳癌における癌関連代謝産物の高効率マッピング実証(OP-062 乳腺 基礎,一般演題,第114回日本外科学会定期学術集会)

    寅田 信博, 久保 真, 三浦 大典, 大内田 研宙, 水内 祐介, 藤村 由紀, 入江 美穂, 大塚 隆生, 仲田 興平, 前山 良, 宮坂 義浩, 小田 義直, 水元 一博, 田中 雅夫

    日本外科学会雑誌   2014.3

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  • 質量分析イメージングによる傷害ストレス条件下でのトマト果実代謝物分布の可視化

    中村純也, 三浦大典, 藤村由紀, 割石博之

    日本農芸化学会大会講演要旨集(Web)   2014.3

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  • 凍結組織アレイおよび質量分析イメージングをもちいた乳癌における癌関連代謝産物の高効率マッピング実証

    寅田信博, 久保真, 三浦大典, 大内田研宙, 水内祐介, 藤村由紀, 入江美穂, 大塚隆生, 仲田興平, 前山良, 宮坂義浩, 小田義直, 水元一博, 田中雅夫

    日本外科学会雑誌   2014.3

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  • 緑茶カテキンEGC代謝の可視化に向けた質量分析イメージング法の開発

    佐々木雅子, 金允喜, 藤村由紀, 行平大地, 山口真一, 齋藤和徳, 三浦大典, 割石博之, 山田耕路, 立花宏文

    日本食品科学工学会西日本支部大会講演要旨   2013.10

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  • 質量分析イメージング法を用いた緑茶カテキンならびにその代謝産物の生体組織内分布の非標識可視化

    金允喜, 藤村由紀, 萩原立春, 佐々木雅子, 行平大地, 齋藤和徳, 三浦大典, 割石博之, 山田耕路, 立花宏文

    日本食品科学工学会西日本支部大会講演要旨   2013.10

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  • 質量分析イメージングを用いた植物代謝物分布の可視化

    中村純也, 三浦大典, 高橋勝利, 藤村由紀, 割石博之

    質量分析総合討論会講演要旨集   2013.9

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  • 2P-187 栄養環境を感知する大腸菌代謝システムの代謝物ネットワーク構造(システムバイオロジー,一般講演)

    行平 大地, 三浦 大典, 藤村 由紀, 割石 博之

    日本生物工学会大会講演要旨集   2013.8

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  • 質量分析イメージングによる熟度に応じたトマト果実代謝物分布の比較

    中村純也, 三浦大典, 高橋勝利, 藤村由紀, 割石博之

    日本生物工学会大会講演要旨集   2013.8

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  • メトホルミンとリラグルチドの添加効果:PKC‐NAD(P)Hオキシダーゼの経路を介して酸化ストレスを減少させる。

    BATTSETSEG Batchuluun, 井口登與志, 園田紀之, 佐々木修二, 井上智彰, 藤村由紀, 三浦大典, 高柳涼一

    糖尿病合併症   2013.8

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  • 3P-031 シスプラチン誘発急性腎不全における代謝変動解析

    入江 美穂, 本田 洋平, 藤村 由紀, 瀬戸山 大樹, 兵藤 文紀, 三浦 大典, 割石 博之

    日本生物工学会大会講演要旨集   2013.8

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  • 低分子食品成分の組織内空間分布の非標識可視化戦略

    藤村由紀, 三浦大典, 割石博之, 立花宏文

    日本栄養・食糧学会大会講演要旨集   2013.4

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  • 組織内微小領域における機能性食品因子の時空間分解可視化

    藤村由紀, 三浦大典, 割石博之, 立花宏文

    日本農芸化学会大会講演要旨集(Web)   2013.3

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  • 質量分析イメージングを用いたトマト果実における代謝物分布の可視化

    中村純也, 加来麻衣子, 藤村由紀, 高橋勝利, 三浦大典, 割石博之

    日本農芸化学会大会講演要旨集(Web)   2013.3

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  • 質量分析イメージング法による緑茶カテキンおよびその代謝物の生体組織内分布情報の非標識可視化

    KIM Yoonhee, 藤村由紀, 萩原立春, 佐々木雅子, 行平大地, 山口歩, 永尾達彦, 齋藤和徳, 三浦大典, 割石博之, 田中浩士, 山田耕路, 立花宏文

    日本農芸化学会大会講演要旨集(Web)   2013.3

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  • Metabolome Early Response to Hydrogen Peroxide-Induced Oxidative Stress in Mamalinan Cell

    Daiki Setoyama, Yoshinori Fujimura, Daisuke Miura

    FREE RADICAL BIOLOGY AND MEDICINE   2012.11

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    DOI: 10.1016/j.freeradbiomed.2012.10.325

  • 3Ga14 質量分析統合利用による網羅的時空間分解代謝変動の解析(オミクス解析,一般講演)

    入江 美穂, 藤村 由紀, 三浦 大典, 大和 真由美, 割石 博之

    日本生物工学会大会講演要旨集   2012.9

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  • 超高分解能質量分析を用いた組成式決定法の理論的検証

    永尾達彦, 行平大地, 藤村由紀, 三浦大典, 割石博之

    日本生物工学会大会講演要旨集   2012.9

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  • 緑茶カテキンEGCのin situ質量分析イメージング法の開発

    佐々木雅子, 金允喜, 藤村由紀, 行平大地, 山口歩, 三浦大典, 割石博之, 山田耕路, 立花宏文

    日本農芸化学会西日本支部大会およびシンポジウム講演要旨集   2012.9

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  • IgE産生阻害緑茶成分ストリクチニンのin situ質量分析イメージング法の開発

    金允喜, 藤村由紀, 行平大地, 山口歩, 三浦大典, 割石博之, 山田耕路, 立花宏文

    日本農芸化学会西日本支部大会およびシンポジウム講演要旨集   2012.9

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  • 3Ga15 MALDI-MSによる代謝物/タンパク質統合イメージング技術の開発(オミクス解析,一般講演)

    山口 歩, 三浦 大典, 藤村 由紀, 割石 博之

    日本生物工学会大会講演要旨集   2012.9

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  • LC/MSを用いたメタボリック・プロファイリング法の応用 ~農産物の生体調節機能の評価~

    藤村 由紀, 三浦 大典, 割石 博之, 谷川 哲雄, 立花 宏文

    2012.8

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  • レドックス関連疾患の理解とニュートリメタボロミクス

    藤村由紀, 三浦大典, 大和真由実, 兵藤文紀, 安川圭司, 市川和洋, 内海英雄, 割石博之, 立花宏文

    日本酸化ストレス学会学術集会プログラム・抄録集   2012.6

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  • 組織内微小領域における緑茶カテキンの二次元可視化に向けた質量分析イメージング法の開発

    萩原立春, 藤村由紀, KIM Yoon Hee, 行平大地, 山口歩, 三浦大典, 割石博之, 山田耕路, 立花宏文

    日本農芸化学会大会講演要旨集(Web)   2012.3

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  • 緑茶カテキンのin situ質量分析イメージング

    萩原立春, 藤村由紀, 行平大地, 山口歩, 三浦大典, 割石博之, 山田耕路, 立花宏文

    日本農芸化学会西日本支部大会およびシンポジウム講演要旨集   2011.9

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  • 超高分解能質量分析による組成式決定アルゴリズムの開発

    永尾達彦, 行平大地, 藤村由紀, 三浦大典, 割石博之

    質量分析総合討論会講演要旨集   2011.9

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  • 質量分析によるmulti‐omicsイメージング

    山口歩, 三浦大典, 藤村由紀, 割石博之

    質量分析総合討論会講演要旨集   2011.9

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  • 緑茶カテキンEGCGの抗腫瘍効果に対する酸素応答性の理解に向けた細胞内代謝物プロファイリング

    藤村由紀, 塚本俊太郎, 入江美穂, 三浦大典, 割石博之, 立花宏文

    日本栄養・食糧学会大会講演要旨集   2011.4

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  • メタボロミクスを駆動力とする緑茶品種の新たな機能性評価

    藤村由紀, 栗原佳奈, 井田恵, 高坂玲亜, 三浦大典, 割石博之, 山本(前田)万里, 根角厚司, 山田耕路, 立花宏文

    日本農芸化学会大会講演要旨集   2011.3

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  • 酸化ストレス疾患のためのOMRI/MSI法の開発

    兵藤文紀, 三浦大典, 藤村由紀, 酒井浄, 市川和洋, 割石博之, 内海英雄

    日本酸化ストレス学会学術集会プログラム・抄録集   2010.6

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  • メタボリック・プロファイリングによる血管内皮障害抑制作用を有する茶葉成分の探索

    栗原佳奈, 井田恵, 藤村由紀, 高坂玲亜, 三浦大典, 割石博之, 山本(前田)万里, 根角厚司, 山田耕路, 立花宏文

    日本栄養・食糧学会大会講演要旨集   2010.5

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  • OP-067-1 メタボリック・プロファイリング法による膵癌の治療抵抗性に関わる代謝産物の解析(膵癌-2,一般口演,第110回日本外科学会定期学術集会)

    大内田 研宙, 藤村 由紀, 三浦 大典, 池永 直樹, 村田 正治, 田上 和夫, 永井 英司, 水元 一博, 田中 雅夫, 割石 博之, 橋爪 誠

    日本外科学会雑誌   2010.3

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  • メタボリック・プロファイリングによる新規高アントシアニン系の活性成分の探索

    栗原佳奈, 井田恵, 藤村由紀, 高坂玲亜, 三浦大典, 割石博之, 山本(前田)万里, 根角厚司, 山田耕路, 立花宏文

    日本農芸化学会大会講演要旨集   2010.3

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  • 機能性食品成分の標的解析戦略:フードケミカルバイオロジー

    藤村 由紀, 山田 耕路, 立花 宏文

    生物機能研究会誌   2009.12

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  • NMRと質量分析を用いた高精度メタボリック・プロファイリング法

    藤村由紀, 三浦大典, 栫井聡子, 須永絵理, 根本直, 高橋勝利, 割石博之

    2009.11

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  • OMRI/MSIによるレドックスプローブのin vivo/in situ解析

    兵藤文紀, 三浦大典, 藤村由紀, 安川圭司, 酒井浄, 市川和洋, 割石博之, 内海英雄

    電子スピンサイエンス学会年会講演要旨集   2009.11

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  • MALDI‐MSを用いたメタボロミクスイメージング

    三浦大典, 藤村由紀, 兵藤文紀, 大和真由実, 立花宏文, 割石博之

    日本農芸化学会関西支部講演会講演要旨集   2009.10

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  • 緑茶カテキン投与マウス尿のメタボリック・プロファイリング

    藤村由紀, 三浦大典, 李珠恵, 邊義こう, 立花宏文, 割石博之

    日本農芸化学会関西支部講演会講演要旨集   2009.10

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  • 新規高アントシアニン茶のメタボリック・プロファイリング

    井田恵, 藤村由紀, 栗原佳奈, 高坂玲亜, 三浦大典, 割石博之, 山本(前田)万里, 山田耕路, 立花宏文

    日本農芸化学会関西支部講演会講演要旨集   2009.10

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  • メタボリック・プロファイリング法によるシスプラチン誘導性酸化ストレスに対する緑茶ポリフェノールの活性評価

    三浦大典, 藤村由紀, 兵藤文紀, 行平大地, 立花宏文, 割石博之

    日本酸化ストレス学会学術集会プログラム・抄録集   2009.6

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  • プロシアニジンの機能性発現における緑茶カテキン受容体67LRの関与

    栗原 佳奈, 藤村 由紀, 和田 哲哉, 山田 耕路, 立花 宏文

    日本栄養・食糧学会大会講演要旨集   2009.5

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  • 緑茶ポリフェノールEGCGのニュートリメタボロミクス

    藤村由紀, 根本直, 須永絵理, 三浦大典, 李珠恵, 邊義こう, 立花宏文, 割石博之

    日本農芸化学会大会講演要旨集   2009.3

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  • 植物性食品成分の生体応答の分子機構

    藤村 由紀, 立花 宏文

    化学と生物   2009.2

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    DOI: 10.1271/kagakutoseibutsu.47.111

  • ウリ科野菜カックロールの抗アレルギー作用

    井田 恵, 藤村 由紀, 矢野 知美, 山田 耕路, 立花 宏文

    日本栄養・食糧学会大会講演要旨集   2008.4

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  • Aggregated ursolic acid, a natural triterpenoid, induces IL-1β release from murine peritoneal macrophages: Role of CD36

    2007.4

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    IL-1β has been shown to play a pivotal role in the development of inflammatory disorders. We recently found that a natural triterpene, ursolic acid (UA), enhanced MIF release from nonstimulated macrophages. In this study, we examined the effects of UA on the production of several cytokines in resident marine peritoneal macrophages (pMφ). UA increased the protein release of IL-1β, IL-6, and MIF, but not of TNF-α, in dose- and time-dependent manners. This triterpene also strikingly induced the activation of p38 MAPK and ERK1/2 together with that of upstream kinases. The release of UA-induced IL-1β was significantly inhibited by the inhibitors of p38 MAPK, MEK1/2, ATP-binding cassette transporter, and caspase-1. Furthermore, UA induced intracellular ROS generation for IL-1β production, which was suppressed by an antioxidant. Pretreatment with an anti-CD36 Ab significantly suppressed IL-1β release, and surface plasmon resonance assay results showed that UA bound to CD36 on macrophages. In addition, the amount of IL-1β released from UA-treated pMφ of CD36-deficient mice was markedly lower than that from those of wild-type mice. Interestingly, UA was found to aggregate in culture medium, and the aggregates were suggested to be responsible for IL-1β production. In addition, i.p. administration of UA increased the levels of IL-1β secretion and MPO activity in colonic mucosa of ICR mice. Taken together, our results indicate that aggregated UA is recognized, in part, by CD36 on macrophages for generating ROS, thereby activating p38 MAPK, ERK1/2, and caspase-1, as well as releasing IL-1β protein via the ATP-binding cassette transporter. Copyright © 2007 by The American Association of Immunologists, Inc.

  • 茶葉成分StrictininのIgE産生抑制機構 細胞膜非ラフト領域を介したSTAT6活性化阻害

    藤村 由紀, 二宮 悠, 長谷川 祐介, 山田 耕路, 立花 宏文

    日本栄養・食糧学会大会講演要旨集   2007.4

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  • Zeaxanthinの高親和性IgE受容体発現抑制作用

    長谷川 祐介, 山里 宣子, 藤村 由紀, 山田 耕路, 立花 宏文

    日本栄養・食糧学会大会講演要旨集   2007.4

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  • メチル化カテキンの脱顆粒抑制には67kDaラミニン受容体を介したミオシン軽鎖のリン酸化低下が関与する

    藤村 由紀, 梅田 大介, 山本 万里[前田], 山田 耕路, 立花 宏文

    日本栄養・食糧学会大会講演要旨集   2006.4

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  • 緑茶カテキンEGCGはマウス前駆脂肪細胞株3T3-L1に緑茶カテキン受容体を介して結合する

    鶴田 小百合, 立花 宏文, 藤村 由紀, 山田 耕路

    日本栄養・食糧学会大会講演要旨集   2006.4

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  • 学会見聞記・日本食品免疫学会 第1回学術大会 (JAFI2005)「食品科学と免疫学の融合をめざして」

    藤村 由紀

    バイオサイエンスとインダストリー   2006.2

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  • お茶に秘められた健康増進効果を探る

    藤村 由紀, 山田 耕路, 立花 宏文

    純真女子短期大学紀要   2004.12

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  • Negative regulation of the basophil activa- tion by natural ligands for Peroxisome proliferator-activated receptors.

    Fujimura Y, Tachibana H, Yamada K

    Animal Cell Technology   2004.5

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  • A receptor for green tea polyphenol EGCG

    H Tachibana, K Koga, Y Fujimura, K Yamada

    NATURE STRUCTURAL & MOLECULAR BIOLOGY   2004.4

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    DOI: 10.1038/nsmb743

  • 豆サポニンの免疫調節機能:高親和性IgE受容体の発現抑制作用

    立花 宏文, 藤村 由紀, 矢野 知美, 山田 耕路

    大豆タンパク質研究   2003.5

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  • ヒト好塩基球様細胞株の高親和性IgE受容体FcεRI発現に及ぼす大豆サポニンの影響

    藤村 由紀, 立花 宏文, 山田 耕路

    日本栄養・食糧学会大会講演要旨集   2003.4

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  • ヒト好塩基球における高親和性IgE受容体発現の制御.

    藤村 由紀, 山田 耕路, 立花 宏文

    生物機能研究会誌   2002.12

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  • Antiallergic tea catechin, (-)-epigallocatechin-3-O-(3-O-methyl)-gallate, suppresses Fc epsilon RI expression in human basophilic KU812 cells

    Y Fujimura, H Tachibana, M Maeda-Yamamoto, T Miyase, M Sano, K Yamada

    JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY   2002.9

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    DOI: 10.1021/jf025680z

  • Antiallergic tea catechin, (-)-epigallocatechin-3-O-(3-O-methyl)-gallate, suppresses FcεRl expression in human basophilic KU812 cells

    2002.9

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    We previously found that the O-methylated derivative of (-)-epigallocatechin-3-O-gallate (EGCg), (-)-epigallocatechin-3-O-(3-O-methyl)-gallate (EGCG″3Me), has potent antiallergic activity. The high-affinity IgE receptor, FcεRI, is found at high levels on basophils and mast cells and plays a key role in a series of acute and chronic human allergic reactions. To understand the mechanism of action for the antiallergic EGCG″3Me, the effect of EGCG″3Me on the cell surface expression of FcεRI in human basophilic KU812 cells was examined. Flow cytometric analysis showed that EGCG″3Me was able to decrease the cell surface expression of FcεRI. Moreover, immunoblot analysis revealed that total cellular expression of the FcεRI α chain decreased upon treatment with EGCG″3Me. FcεRI is a tetrameric structure comprising one α chain, one β chain, and two γ chains. The level of mRNA production of each subunit in KU812 cells was investigated. EGCG″3Me reduced FcεRI α and γ mRNA levels. The cross-linkage of FcεRI causes the activation of basophils, which leads to the secretion of inflammatory mediators including histamine. EGCG″3Me treatment inhibited the FcεRI cross-linking-induced histamine release. These results suggested that EGCGε3Me can negatively regulate basophil activation through the suppression of FcεRI expression.

    DOI: 10.1021/jf025680z

  • ヒト好塩基球様細胞株KU812の高親和性IgE受容体発現に対するTransferrinの作用

    藤村 由紀, 立花 宏文, 山田 耕路

    日本農芸化学会誌   2001.2

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  • Antigen binding of an ovomucoid-specific antibody is affected by a carbohydrate chain located on the light chain variable region

    Y Fujimura, H Tachibana, N Eto, K Yamada

    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY   2000.11

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    DOI: 10.1271/bbb.64.2298

  • 食物アレルゲン(オボムコイド)特異的ヒト型化抗体の作製

    藤村 由紀, 立花 宏文, 江藤 望, 山田 耕路

    日本農芸化学会誌   1998.12

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Industrial property rights

Patent   Number of applications: 5   Number of registrations: 0
Utility model   Number of applications: 0   Number of registrations: 0
Design   Number of applications: 0   Number of registrations: 0
Trademark   Number of applications: 0   Number of registrations: 0

Professional Memberships

  • フードサイエンスフォーラム

  • 日本食品免疫学会

  • 日本栄養・食糧学会

  • 日本農芸化学会

  • 日本フードファクター学会

  • 日本カテキン学会

  • THE MASS SPECTROMETRY SOCIETY OF JAPAN

  • 日本食品科学工学会

  • フォードサイエンスフォーラム

  • 日本食品科学工学会

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  • 日本食品免疫学会

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  • 日本農芸化学会

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  • THE MASS SPECTROMETRY SOCIETY OF JAPAN

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  • 日本栄養・食糧学会

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  • 日本フードファクター学会

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  • 日本カテキン学会

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  • フォードサイエンスフォーラム

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Committee Memberships

  • Councilor   Domestic

    2022.3 - 2024.2   

  • 日本食品科学工学会   評議員  

    2020.3 - 2024.2   

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  • 日本農芸化学会西日本支部   幹事   Domestic

    2019.5 - 2025.4   

  • 日本農芸化学会   西日本支部幹事  

    2019.5 - 2025.2   

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  • 日本農芸化学会   和文誌編集委員会委員   Domestic

    2019.3 - 2023.2   

  • 日本農芸化学会   和文誌編集委員会委員  

    2019.3 - 2023.2   

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  • 日本フードファクター学会   評議員  

    2018.4 - Present   

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    Committee type:Academic society

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  • Councilor   Domestic

    2015.12 - 2023.3   

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Academic Activities

  • 進行役

    日本農芸化学会  ( オンライン ) 2023.3

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    Type:Competition, symposium, etc. 

  • Screening of academic papers

    Role(s): Peer review

    2023

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    Type:Peer review 

    Number of peer-reviewed articles in foreign language journals:4

    Number of peer-reviewed articles in Japanese journals:3

  • 座長

    第27回日本フードファクター学会学術集会  ( 東京都 ) 2022.10

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    Type:Competition, symposium, etc. 

    Number of participants:350

  • 第27回日本フードファクター学会学術集会

    Role(s): Panel moderator, session chair, etc.

    日本フードファクター学会  2022.10

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  • 大会実行委員、世話人

    第59回化学関連支部合同九州大会  ( 北九州市 ) 2022.7

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    Type:Competition, symposium, etc. 

    Number of participants:900

  • 第59回化学関連支部合同九州大会

    Role(s): Planning, management, etc.

    日本農芸化学会西日本支部  2022.7

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    Type:Academic society, research group, etc. 

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  • プログラム委員、セッションオーガナイザー、座長

    日本質量分析学会 第70回質量分析総合討論会  ( 福岡市 ) 2022.6

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    Type:Competition, symposium, etc. 

    Number of participants:700

  • 第70回質量分析総合討論会

    Role(s): Planning, management, etc., Panel moderator, session chair, etc.

    日本質量分析学会  2022.6

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    Type:Academic society, research group, etc. 

    researchmap

  • Screening of academic papers

    Role(s): Peer review

    2022

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    Type:Peer review 

    Number of peer-reviewed articles in foreign language journals:12

    Number of peer-reviewed articles in Japanese journals:8

  • 座長

    日本農芸化学会  ( 仙台市 ) 2021.3

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    Type:Competition, symposium, etc. 

  • Screening of academic papers

    Role(s): Peer review

    2021

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    Type:Peer review 

    Number of peer-reviewed articles in foreign language journals:15

    Number of peer-reviewed articles in Japanese journals:5

  • 大会実行委員会委員

    2020年度日本フードファクター学会・日本農芸化学会西日本支部合同大会  ( 宮崎市 ) 2020.11

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    Type:Competition, symposium, etc. 

  • 座長

    日本食品科学工学会西日本支部大会  ( 福岡市 ) 2020.11

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    Type:Competition, symposium, etc. 

  • 企画立案・運営等

    日本食品科学工学会 第67回大会  ( 福岡市 Japan ) 2020.8

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    Type:Competition, symposium, etc. 

  • 企画立案・運営等, パネル司会・セッションチェア等

    日本農芸化学会  ( 福岡市 Japan ) 2020.3

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    Type:Competition, symposium, etc. 

  • Screening of academic papers

    Role(s): Peer review

    2020

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    Type:Peer review 

    Number of peer-reviewed articles in foreign language journals:11

    Number of peer-reviewed articles in Japanese journals:2

  • 企画立案・運営等, パネル司会・セッションチェア等 International contribution

    ( 神戸市 Japan ) 2019.12

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    Type:Competition, symposium, etc. 

  • パネル司会・セッションチェア等 International contribution

    ( 神戸市 Japan ) 2019.11 - 2019.12

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    Type:Competition, symposium, etc. 

  • 企画立案・運営等, パネル司会・セッションチェア等

    日本プロテオーム学会・日本電気泳動学会総会(JPrOS2019/JES2019)  ( 宮崎市 Japan ) 2019.7

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    Type:Competition, symposium, etc. 

  • パネル司会・セッションチェア等

    日本農芸化学会  ( 東京都 Japan ) 2019.3

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    Type:Competition, symposium, etc. 

  • 化学と生物

    2019.2 - 2020.1

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    Type:Academic society, research group, etc. 

  • Screening of academic papers

    Role(s): Peer review

    2019

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    Type:Peer review 

    Number of peer-reviewed articles in foreign language journals:12

    Number of peer-reviewed articles in Japanese journals:0

    Proceedings of International Conference Number of peer-reviewed papers:0

    Proceedings of domestic conference Number of peer-reviewed papers:0

  • 座長

    日本食品科学工学会西日本支部大会  ( 福岡市 ) 2018.11

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    Type:Competition, symposium, etc. 

  • 座長

    日本農芸化学会  ( 名古屋市 ) 2018.3

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    Type:Competition, symposium, etc. 

  • Screening of academic papers

    Role(s): Peer review

    2018

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    Type:Peer review 

    Number of peer-reviewed articles in foreign language journals:12

    Number of peer-reviewed articles in Japanese journals:0

    Proceedings of International Conference Number of peer-reviewed papers:0

    Proceedings of domestic conference Number of peer-reviewed papers:0

  • 座長(Chairmanship)

    日本農芸化学会  ( 京都市 ) 2017.3

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    Type:Competition, symposium, etc. 

  • Screening of academic papers

    Role(s): Peer review

    2017

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    Type:Peer review 

    Number of peer-reviewed articles in foreign language journals:11

    Number of peer-reviewed articles in Japanese journals:1

    Proceedings of International Conference Number of peer-reviewed papers:1

    Proceedings of domestic conference Number of peer-reviewed papers:1

  • 平成26年度補正予算「技術革新を加速化する最先端分析技術の応用研究支援事業」評価委員

    Role(s): Review, evaluation

    農林水産省  2016.6 - 2017.3

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    Type:Scientific advice/Review 

  • 座長(Chairmanship)

    日本農芸化学会  ( 札幌市 ) 2016.3

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    Type:Competition, symposium, etc. 

  • 世話人

    日本農芸化学会  ( 札幌市 ) 2016.3

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    Type:Competition, symposium, etc. 

    Number of participants:2,000

  • Screening of academic papers

    Role(s): Peer review

    2016

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    Type:Peer review 

    Number of peer-reviewed articles in foreign language journals:14

    Number of peer-reviewed articles in Japanese journals:0

    Proceedings of International Conference Number of peer-reviewed papers:0

    Proceedings of domestic conference Number of peer-reviewed papers:0

  • 座長(Chairmanship)

    第12回日本カテキン学会年次学術集会  ( 福岡市 ) 2015.12

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    Type:Competition, symposium, etc. 

  • 実行委員

    第12回日本カテキン学会年次学術集会  ( 福岡市 ) 2015.12

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    Type:Competition, symposium, etc. 

    Number of participants:100

  • 座長(Chairmanship)

    日本農芸化学会  ( 岡山市 ) 2015.3

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    Type:Competition, symposium, etc. 

  • 平成26年度補正予算「技術革新を加速化する最先端分析技術の応用研究支援事業」審査委員

    Role(s): Review, evaluation

    農林水産省  2015.2 - 2016.7

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    Type:Scientific advice/Review 

  • 座長(Chairmanship)

    第8回レドックス・ライフイノベーション第170委員会  ( 宮崎市 ) 2014.8

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    Type:Competition, symposium, etc. 

  • 特別研究員等審査会専門委員及び国際事業委員会書面審査員・書面評価員

    Role(s): Review, evaluation

    日本学術振興会  2014.8 - 2016.7

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    Type:Scientific advice/Review 

  • 座長(Chairmanship)

    日本農芸化学会  ( 川崎市 ) 2014.3

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    Type:Competition, symposium, etc. 

  • 実行委員

    メタボロームシンポジウム2013若手会  ( 福岡県糟屋郡篠栗 ) 2013.10

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    Type:Competition, symposium, etc. 

    Number of participants:45

  • 座長(Chairmanship)

    第8回メタボロームシンポジウム  ( 福岡市 ) 2013.10

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    Type:Competition, symposium, etc. 

  • 実行委員

    第8回メタボロームシンポジウム  ( 福岡市 ) 2013.10

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    Type:Competition, symposium, etc. 

    Number of participants:300

  • 座長(Chairmanship)

    平成24年度日本農芸化学会西日本支部および日本栄養食糧学会九州・沖縄支部合同大会  ( 鹿児島市 ) 2012.9

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    Type:Competition, symposium, etc. 

  • 座長(Chairmanship)

    日本農芸化学会西日本支部・中四国支部合同大会  ( 宮崎市 ) 2011.9

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    Type:Competition, symposium, etc. 

  • 座長(Chairmanship)

    日本農芸化学会  ( 京都市 ) 2011.3

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    Type:Competition, symposium, etc. 

  • 座長(Chairmanship) International contribution

    2010.9

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    Type:Competition, symposium, etc. 

  • 座長(Chairmanship)

    日本農芸化学会関西・中四国・西日本支部、日本栄養食糧学会九州沖縄支部、日本食品科学工学会西日本支部合同大会  ( 沖縄 ) 2009.10 - 2009.11

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    Type:Competition, symposium, etc. 

  • 座長(Chairmanship)

    日本農芸化学会  ( 東京 ) 2007.3

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    Type:Competition, symposium, etc. 

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Research Projects

  • Approaching the reality of phytochemical boosting regulated by plant-derived functional RNAs

    Grant number:24K21841  2024.6 - 2026.3

    Grants-in-Aid for Scientific Research  Grant-in-Aid for Challenging Research (Exploratory)

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    Grant type:Scientific research funding

    CiNii Research

  • Anti-fibrotic effect of microRNAs in NASH models

    Grant number:24K08835  2024.4 - 2027.3

    Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

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    Grant type:Scientific research funding

    CiNii Research

  • フードペアリングがもたらす潜在的リスク・保健効果の高感度評価技術の開発

    Grant number:23H02163  2023 - 2025

    日本学術振興会  科学研究費助成事業  基盤研究(B)

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    Authorship:Principal investigator  Grant type:Scientific research funding

  • ファイトケミカルの肝線維化改善作用の理解に資する非コードRNAと代謝物の統合解析

    Grant number:22K05525  2022 - 2024

    日本学術振興会  科学研究費助成事業  基盤研究(C)

    一瀬 智美, 藤村 由紀

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    Authorship:Coinvestigator(s)  Grant type:Scientific research funding

    本研究では、肝線維化の制御因子として期待されている「非コードRNA(環状/マイクロRNA)の発現プロファイル」 と 「病態に関連した代謝変動」 を統合的に解析することで、線維化における病態形成機序の一端を解明する。また、線維化病態に特徴的な非コードRNAと代謝物の挙動を指標とすることで、現状では科学的エビデンスの乏しい植物由来生理活性成分(ファイトケミカル)の肝線維化改善メカニズムを明らかにする。

    CiNii Research

  • Capturing the behavior of functional RNAs across the plant-animal boundary contributing to bioregulatory actions

    Grant number:22K19154  2022 - 2023

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Challenging Research(Exploratory)

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    Authorship:Principal investigator  Grant type:Scientific research funding

    CiNii Research

  • 新規フードファクターとしての抹茶由来機能性 RNA の生体調節作用の解析

    2022 - 2023

    抹茶と健康研究会 研究助成

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    Authorship:Principal investigator  Grant type:Contract research

  • 抹茶中の新たな機能性食品因子の発掘に向けた機能性RNAの解析

    2021 - 2022

    抹茶と健康研究会 研究助成

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    Authorship:Principal investigator  Grant type:Contract research

  • Integrated understanding of food functional responsible factors and their functional interactions

    Grant number:20H05683  2020 - 2024

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (S)

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    Authorship:Coinvestigator(s)  Grant type:Scientific research funding

    CiNii Research

  • Multi-omics analysis of dietary microRNAs and metabolites toward functional food pairing

    Grant number:20H02935  2020 - 2022

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (B)

    Fujimura Yoshinori

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    Authorship:Principal investigator  Grant type:Scientific research funding

    From the viewpoint that food functionality is based on the cooperative action of a series of components, we verified the multifaceted interrelationships among components that contribute to "functional food pairing," a combination of food that enables us to receive the functionality that manifests when specific components are combined. Through metabolic profiling analysis using a green tea extract library, we were able to propose functionality-related polyphenol components and useful combinations of components that enhance functionality. In addition, we discovered novel signal molecules capable of sensing functional components for effectively extracting the potential combinations. We also identified novel biomarkers that contribute to risk assessment of functional components. Furthermore, we identified various microRNAs as candidates of functional components in green tea, and clarified the functionality of plant-derived microRNAs.

    CiNii Research

  • Development of Ultrahigh-throughput Technology for Single cell Metabolic Phenotyping

    Grant number:20H02872  2020 - 2022

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (B)

    Miura Daisuke

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    Authorship:Coinvestigator(s)  Grant type:Scientific research funding

    In this study, we have investigated the use of W/O droplets mimicking animal cells in order to optimize the sample preparation method with high sensitivity and reproducibility, aiming to establish a metabolic profiling method for single cells using MALDI-MS. We succeeded in establishing a sample preparation method with high reproducibility and high sensitivity by providing a matrix by vapor deposition followed by recrystallization under mild conditions. Using these conditions, the droplet containing multiple metabolite standards can be detected independently by mass spectrometry, and the detection sensitivity was found to be very favorable, with detection at the atto mol level being sufficient.

    CiNii Research

  • 生体レドックス制御に有効な食品成分コンビネーションの革新的提示・予測法の創出

    Grant number:17H03819  2017 - 2019

    日本学術振興会  科学研究費助成事業  基盤研究(B)

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    Authorship:Principal investigator  Grant type:Scientific research funding

  • 生体内因性分子をプローブとする磁気共鳴代謝イメージング法の開発

    Grant number:16H05079  2016 - 2018

    日本学術振興会  科学研究費助成事業  基盤研究(B)

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    Authorship:Coinvestigator(s)  Grant type:Scientific research funding

  • 空間分解メタボロミクスによる植物代謝動態可視化

    Grant number:15K14921  2015 - 2016

    科学研究費助成事業  挑戦的萌芽研究

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    Authorship:Coinvestigator(s)  Grant type:Scientific research funding

  • 健康長寿食を支える抗酸化物質の根拠不在な生体内活性の革新的解析法の創出

    Grant number:26282025  2014 - 2016

    日本学術振興会  科学研究費助成事業  基盤研究(B)

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    Authorship:Principal investigator  Grant type:Scientific research funding

  • 経口摂取分子群の一斉動態イメージングに向けた基盤技術の開発

    Grant number:26660115  2014 - 2015

    科学研究費助成事業  挑戦的萌芽研究

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    Authorship:Principal investigator  Grant type:Scientific research funding

  • 機能性食品成分の超簡便・超高感度センシング技術の開発

    2013

    H25 研究成果最適展開支援プログラム(A-STEP) FSステージ 探索タイプ

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    Authorship:Principal investigator  Grant type:Contract research

  • メタボロミクスを基盤とする機能性食品因子の高解像度生体応答解析

    Grant number:24658124  2012 - 2013

    科学研究費助成事業  挑戦的萌芽研究

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    Authorship:Principal investigator  Grant type:Scientific research funding

  • 高品質な食品の機能的デザインを支援する多次元情報統合化技術の開発

    2012 - 2013

    平成24年度 研究成果最適展開支援事業 (A-STEP) FS 探索タイプ

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    Authorship:Principal investigator  Grant type:Contract research

  • 健康維持増進に資する食シグナル応答の包括的理解に向けた革新的多分子可視化戦略

    Grant number:23680073  2011 - 2013

    科学研究費助成事業  若手研究(A)

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    Authorship:Principal investigator  Grant type:Scientific research funding

  • 多次元メタボロミクスイメージングによる次世代型食品機能性評価システムの開発

    2011

    H23 研究成果最適展開支援プログラム(A-STEP) FSステージ 探索タイプ

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    Authorship:Principal investigator  Grant type:Contract research

  • 分子疫学とケミカルバイオロジーを駆動力とする食品因子感知システムの解明

    Grant number:22228002  2010 - 2014

    日本学術振興会  科学研究費助成事業  基盤研究(S)

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    Authorship:Coinvestigator(s)  Grant type:Scientific research funding

  • ケミカルバイオロジーと分子疫学的解析に基づく機能性食品因子感知システムの解明

    Grant number:22248015  2010 - 2013

    日本学術振興会  科学研究費助成事業  基盤研究(A)

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    Authorship:Coinvestigator(s)  Grant type:Scientific research funding

  • レドックスイメージングとMALDI質量分析による遺伝子治療応答性の非侵襲的評価法

    Grant number:22659111  2010 - 2012

    科学研究費助成事業  挑戦的萌芽研究

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    Authorship:Coinvestigator(s)  Grant type:Scientific research funding

  • ニュートリメタボロミクスを切り拓く高精度質量分析システムの開発

    Grant number:22658043  2010 - 2011

    科学研究費助成事業  挑戦的萌芽研究

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    Authorship:Principal investigator  Grant type:Scientific research funding

  • 食品機能性評価に向けた代謝物プロファイリング技術の開発

    2010

    H22 研究成果最適展開支援プログラム(A-STEP) FSステージ 探索タイプ

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    Authorship:Principal investigator  Grant type:Contract research

  • 緑茶カテキン受容体を介したカテキンの機能性発現とシグナリングの統合解析

    Grant number:18208012  2006 - 2009

    日本学術振興会  科学研究費助成事業  基盤研究(A)

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    Authorship:Coinvestigator(s)  Grant type:Scientific research funding

  • 食品成分の標的受容体分子の機能制御に基づく抗アレルギーシグナルの解明

    Grant number:17780111  2005 - 2007

    科学研究費助成事業  若手研究(B)

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    Authorship:Principal investigator  Grant type:Scientific research funding

▼display all

Class subject

  • 食品機能学

    2024.10 - 2025.3   Second semester

  • 微生物学基礎実験

    2024.10 - 2025.3   Second semester

  • 食料化学工学演習第二

    2024.10 - 2025.3   Second semester

  • インターンシップ

    2024.4 - 2025.3   Full year

  • 食料化学工学特別研究第二

    2024.4 - 2025.3   Full year

  • 食料化学工学特別研究第一

    2024.4 - 2025.3   Full year

  • 食料化学工学特別研究第一

    2024.4 - 2025.3   Full year

  • 食料化学工学演示技法

    2024.4 - 2025.3   Full year

  • 食料化学工学ティーチング演習

    2024.4 - 2025.3   Full year

  • 食糧化学

    2024.4 - 2024.9   First semester

  • 食糧化学実験

    2024.4 - 2024.9   First semester

  • 食料化学工学プロジェクト演習

    2024.4 - 2024.6   Spring quarter

  • 食品機能学特論

    2024.4 - 2024.6   Spring quarter

  • 食品機能学

    2023.10 - 2024.3   Second semester

  • 食料化学工学演習第二

    2023.10 - 2024.3   Second semester

  • 微生物学基礎実験

    2023.10 - 2024.3   Second semester

  • 食料化学工学特別研究第二

    2023.4 - 2024.3   Full year

  • インターンシップ

    2023.4 - 2024.3   Full year

  • 食料化学工学ティーチング演習

    2023.4 - 2024.3   Full year

  • 食料化学工学演示技法

    2023.4 - 2024.3   Full year

  • 食料化学工学特別研究第一

    2023.4 - 2024.3   Full year

  • 食料化学工学特別研究第一

    2023.4 - 2024.3   Full year

  • 食糧化学実験

    2023.4 - 2023.9   First semester

  • 食糧化学

    2023.4 - 2023.9   First semester

  • 食料化学工学プロジェクト演習

    2023.4 - 2023.6   Spring quarter

  • 食品機能学特論

    2023.4 - 2023.6   Spring quarter

  • 微生物学基礎実験

    2022.10 - 2023.3   Second semester

  • 食品機能学

    2022.10 - 2023.3   Second semester

  • 食料化学工学演習第二

    2022.10 - 2023.3   Second semester

  • 食料化学工学特別研究第二

    2022.4 - 2023.3   Full year

  • インターンシップ

    2022.4 - 2023.3   Full year

  • 食料化学工学ティーチング演習

    2022.4 - 2023.3   Full year

  • 食料化学工学演示技法

    2022.4 - 2023.3   Full year

  • 食料化学工学特別研究第一

    2022.4 - 2023.3   Full year

  • 食料化学工学特別研究第一

    2022.4 - 2023.3   Full year

  • 食糧化学実験

    2022.4 - 2022.9   First semester

  • 食糧化学

    2022.4 - 2022.9   First semester

  • 食品機能学特論

    2022.4 - 2022.6   Spring quarter

  • 食料化学工学プロジェクト演習

    2022.4 - 2022.6   Spring quarter

  • 食料化学工学演習第二

    2021.10 - 2022.3   Second semester

  • 食糧製造化学

    2021.10 - 2022.3   Second semester

  • 微生物学基礎実験

    2021.10 - 2022.3   Second semester

  • インターンシップ

    2021.4 - 2022.3   Full year

  • 食料化学工学特別研究第二

    2021.4 - 2022.3   Full year

  • 食料化学工学特別研究第一

    2021.4 - 2022.3   Full year

  • 食料化学工学特別研究第一

    2021.4 - 2022.3   Full year

  • 食料化学工学演示技法

    2021.4 - 2022.3   Full year

  • 食料化学工学ティーチング演習

    2021.4 - 2022.3   Full year

  • 食糧化学実験

    2021.4 - 2021.9   First semester

  • 食料化学工学プロジェクト演習

    2021.4 - 2021.6   Spring quarter

  • 食品機能学特論

    2021.4 - 2021.6   Spring quarter

  • 食糧製造化学

    2020.10 - 2021.3   Second semester

  • 食料化学工学演習第一

    2020.10 - 2021.3   Second semester

  • 食料化学工学演習第二

    2020.10 - 2021.3   Second semester

  • 微生物学基礎実験

    2020.10 - 2021.3   Second semester

  • 食料化学工学特別研究第二

    2020.4 - 2021.3   Full year

  • インターンシップ

    2020.4 - 2021.3   Full year

  • 食料化学工学ティーチング演習

    2020.4 - 2021.3   Full year

  • 食料化学工学演示技法

    2020.4 - 2021.3   Full year

  • 食料化学工学特別研究第一

    2020.4 - 2021.3   Full year

  • 食糧化学実験

    2020.4 - 2020.9   First semester

  • 食品機能学特論

    2020.4 - 2020.6   Spring quarter

  • G30 Food Science

    2020.4 - 2020.6   Spring quarter

  • 食料化学工学プロジェクト演習

    2020.4 - 2020.6   Spring quarter

  • 食糧製造化学

    2019.10 - 2020.3   Second semester

  • 実地見学(食糧化学工学分野)

    2019.4 - 2020.3   Full year

  • 食糧化学実験

    2019.4 - 2019.9   First semester

  • 食品機能学特論

    2019.4 - 2019.9   First semester

  • 食料化学工学プロジェクト演習

    2019.4 - 2019.9   First semester

  • G30 Food Science

    2019.4 - 2019.6   Spring quarter

  • 食糧製造化学

    2018.10 - 2019.3   Second semester

  • 食品機能学特論

    2018.4 - 2018.9   First semester

  • Food Science and Biotechnology

    2018.4 - 2018.9   First semester

  • 食料化学工学プロジェクト演習

    2018.4 - 2018.9   First semester

  • 食糧化学実験

    2018.4 - 2018.9   First semester

  • G30 Food Science

    2018.4 - 2018.6   Spring quarter

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FD Participation

  • 2019.5   Role:Participation   Title:優良な博士人材の獲得と育成に向けて ~農学研究院教授に学ぶ ~

    Organizer:[Undergraduate school/graduate school/graduate faculty]

  • 2019.2   Role:Participation   Title:九州大学TA制度​に関するFD・SDについて ー大学教育の質向上にむけてー

    Organizer:University-wide

Other educational activity and Special note

  • 2019  Class Teacher 

  • 2018  Class Teacher 

Outline of Social Contribution and International Cooperation activities

  • 機能性食品の研究開発に関する一般市民への講演

Media Coverage

  • 食品ポリフェノールの生体感知機構と機能性増強技術に関する研究 Newspaper, magazine

    2020.8

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    食品ポリフェノールの生体感知機構と機能性増強技術に関する研究

  • 九大、機能性を示す食品成分の組合せを簡便に発見できる技術を開発 Newspaper, magazine

    2017.5

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    九大、機能性を示す食品成分の組合せを簡便に発見できる技術を開発

  • イソ吉草酸配糖体「コーヒーの味を左右」 ~生豆選別の指標に~ Newspaper, magazine

    2017.5

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    イソ吉草酸配糖体「コーヒーの味を左右」 ~生豆選別の指標に~

  • カテキン+果実成分で機能アップ Newspaper, magazine

    2014.10

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    カテキン+果実成分で機能アップ

  • コーヒー香味、熟度と生豆成分に相関性 Newspaper, magazine

    2014.10

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    コーヒー香味、熟度と生豆成分に相関性

  • 「九大、体内分布 目で確認」 質量分析の手法応用 機能性食品評価に一役 Newspaper, magazine

    2013.3

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    「九大、体内分布 目で確認」 質量分析の手法応用 機能性食品評価に一役

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