Publishing type：Research paper (scientific journal)  

Language：Japanese   Publishing type：Research paper (other academic)  

Sialmarine is a soil collected from a stratum approximately 300,000 years old at a depth of 40 m below the surface, and has been reported to have intracellular reactive oxygen species (ROS) scavenging activity. In this study, we examined the anti-tumor effect of this marine soil using mice models of carcinoma and conducted a clinical study on dogs with malignant tumors. Tumor size was measured in each group after Colon-26 cell transplantation, and a Sialmarine content-dependent tumor size suppression was overbred. In clinical trials, one case of tumor shrinkage and another case of prolongation of life and improvement of QOL were observed, although little regression was observed, indicating a certain level of efficacy. And also, almost no side effects were observed.

Language：Japanese   Publisher：動物臨床医学会  

シアルマリン(以下シアルマリン)は地下40mにある約30万年前の地層から採取される堆積物であり、活性酸素消去活性を有していることが報告されている。本研究では、この海洋性堆積物を用いて担ガンモデルマウスを使用した抗腫瘍効果を検証し、併せて悪性腫瘍罹患犬への臨床試験を行った。Colon-26細胞移植後に各郡における腫瘍サイズの計測を行ったところシアルマリン含有量依存的な腫瘍サイズ抑制効果が確認された。臨床試験については腫瘍の縮小が明らかに認められた例、退縮効果はほとんど認められないものの延命やQOLの改善が示唆される例など一定の効果が確認された。また副作用はほとんど認められなかった。(著者抄録)

Language：Japanese   Publishing type：Research paper (other academic)  

Abstract Home electric potential therapy devices are AC electric field (EF) generators that have been approved by the Ministry of Health, Labor, and Welfare in Japan as effective in improving headaches, stiff shoulders, chronic constipation and insomnia. However, while biological studies on electromagnetic fields (EMF) exposure have been widely reported, there are few studies have focused on EF. In this study, we examined the effects of EF exposure on differentiated MC3T3E-1 under extremely low EMF conditions. We found that EF exposure increased the amount of calcification and alkaline phosphatase activity. These results suggest that EF exposure causes increased calcification of osteoblasts

Language：English   Publishing type：Research paper (scientific journal)  

Aging-related neurodegenerative disorders are closely associated with mitochondrial dysfunction and oxidative stresses and their incidence tends to increase with aging. Brain is the most vulnerable to reactive species generated by a higher rate of oxygen consumption and glucose utilization compared to other organs. Electrochemically reduced water (ERW) was demonstrated to scavenge reactive oxygen species (ROS) in several cell types. In the present study, the protective effect of ERW against hydrogen peroxide (H2O2) and nitric oxide (NO) was investigated in several rodent neuronal cell lines and primary cells. ERW was found to significantly suppress H2O2 (50-200 mu M) induced PC12 and SFME cell deaths. ERW scavenged intracellular ROS and exhibited a protective effect against neuronal network damage caused by 200 mu M H2O2 in N1E-115 cells. ERW significantly suppressed NO-induced cytotoxicity in PC12 cells despite the fact that it did not have the ability to scavenge intracellular NO. ERW significantly suppressed both glutamate induced Ca2+ influx and the resulting cytotoxicity in primary cells. These results collectively demonstrated for the first time that ERW protects several types of neuronal cells by scavenging ROS because of the presence of hydrogen and platinum nanoparticles dissolved in ERW.

DOI： 10.1155/2014/869121

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1371/journal.pone.0171192

Language：English   Publishing type：Research paper (scientific journal)  

Antibody display methods are increasingly being used to produce human monoclonal antibodies for disease therapy. Rapid screening and isolation of specific human antibody genes are valuable for producing human monoclonal antibodies showing high specificity and affinity. In this report, we describe a novel mammalian cell display method in which whole human IgG is displayed on the cell surface of CHO cells. Cells expressing antigen-specific human monoclonal IgGs with high affinity on the cell surface after normal folding and posttranscriptional modification were screened using a cell sorter. The membrane-type IgG-expressing CHO cells were then converted to IgG-secreting cells by transfection with a plasmid coding Cre recombinase. This mammalian cell display method was applied to in vitro affinity maturation of monoclonal C9 IgG specific to the human high-affinity IgE receptor (Fc epsilon RI alpha). The CDR3 of the C9 heavy chain variable region gene was randomly mutated and inserted into pcDNA5FRT/IgG. A C9 IgG (CDRH3r)-expressing CHO cell display library consisting of 1.1 x 10(6) independent clones was constructed. IgG-displaying cells showing high reactivity to Fc epsilon RI alpha antigen were screened by the cell sorter, resulting in the establishment of a CHO cell line producing with higher reactivity than the parent C9 IgG. (C) 2013 Elsevier Inc. All rights reserved.

DOI： 10.1016/j.bbrc.2013.10.007

Language：English   Publishing type：Research paper (scientific journal)  

Insulin-producing cells express limited activities of anti-oxidative enzymes. Therefore, reactive oxygen species (ROS) produced in these cells play a crucial role in cytotoxic effects. Furthermore, diabetes mellitus (DM) development is closely linked to higher ROS levels in insulin-producing cells. Hita Tenryosui Water(A (R)) (Hita T. W., Hita, Japan) and Nordenau water (Nord. W., Nordenau, Germany), referred to as natural reduced waters (NRWs), scavenge ROS in cultured cells, and therefore, might be a possibility as an alternative to conventional pharmacological agents against DM. Therefore, this study aimed to investigate the role of NRWs in alloxan (ALX)-induced beta-cell apoptosis as well as in ALX-induced diabetic mice. NRWs equally suppressed DNA fragmentation levels. Hita T. W. and Nord. W. ameliorated ALX-induced sub-G(1) phase production from approximately 40% of control levels to 8.5 and 11.8%, respectively. NRWs restored serum insulin levels (p < 0.01) and reduced blood glucose levels (p < 0.01) in ALX-induced mice. Hita T. W. restored tissue superoxide dismutase (SOD) (p < 0.05) activity but not tissue catalase activity. Hita T. W. did not elevate SOD or catalase activity in HIT-T15 cells. Nord. W. restored SOD (p < 0.05) and catalase (p < 0.05) activity in both cultured cells and pancreatic tissue to normal levels. Even though variable efficacies were observed between Hita T. W. and Nord. W., both waters suppressed ALX-induced DM development in CD-1 male mice by administering NRWs for 8 weeks. Our results suggest that Hita T. W. and Nord. W. protect against ALX-induced beta-cell apoptosis, and prevent the development of ALX-induced DM in experimental animals by regulating ALX-derived ROS generation and elevating anti-oxidative enzymes. Therefore, the two NRWs tested here are promising candidates for the prevention of DM development.

DOI： 10.1007/s10616-011-9414-

Language：English   Publishing type：Research paper (scientific journal)  

It has been demonstrated that hydrogen peroxide (H2O2) is directly associated with elevated matrix metalloproteinase-2 (MMP-2) expression in several cell lines. Electrochemically reduced water (ERW), produced near the cathode during electrolysis, and scavenges intracellular H2O2 in human fibrosarcoma HT1080 cells. RT-PCR and zymography analyses revealed that when HT1080 cells were treated with ERW, the gene expression of MMP-2 and membrane type 1 MMP and activation of MMP-2 was repressed, resulting in decreased invasion of the cells into matrigel. ERW also inhibited H2O2-induced MMP-2 upregulation. To investigate signal transduction involved in MMP-2 downregulation, mitogen-activated protein kinase (MAPK)-specific inhibitors, SB203580 (p38 MAPK inhibitor), PD98059 (MAPK/extracellular regulated kinase kinase 1 inhibitor) and c-Jun NH2-terminal kinase inhibitor II, were used to block the MAPK signal cascade. MMP-2 gene expression was only inhibited by SB203580 treatment, suggesting a pivotal role of p38 MAPK in regulation of MMP-2 gene expression. Western blot analysis showed that ERW downregulated the phosphorylation of p38 both in H2O2-treated and untreated HT1080 cells. These results indicate that the inhibitory effect of ERW on tumor invasion is due to, at least in part, its antioxidative effect.

DOI： 10.1007/s10616-012-9469-7

Language：English   Publishing type：Research paper (scientific journal)  

In Japan, research on functional water, especially on reduced water, is developing at a rapid pace. Reduced water such as electrochemically reduced water and natural reduced water can scavenge reactive oxygen species in cultured cells. Reduced waters are expected to have preventive and positive effects on oxidative stress-related diseases such as diabetes, cancer, arteriosclerosis, neurodegenerative diseases, and side effects of hemodialysis. It has been suggested that the active agents in reduced water are hydrogen (atoms and molecules), mineral nanoparticles, and mineral nanoparticle hydrides.

DOI： 10.1016/j.tifs.2011.10.009

Language：English   Publishing type：Research paper (scientific journal)  

Electrolyzed reduced water (ERW) contains a large amount of molecular hydrogen and a small amount of Pt nanoparticles (Pt NPs). We have found that ERW significantly extended the lifespan of Caenorhabditis elegans in a novel culture medium designated Water Medium. In this study, we found that synthetic Pt NPs at ppb levels significantly extended the nematode lifespan and scavenged reactive oxygen species (ROS) in the nematode induced by paraquat treatment. In contrast, a high concentration of dissolved molecular hydrogen had no significant effect on the lifespan of the nematode. These findings suggest that the Pt NPs in ERW, rather than the molecular hydrogen, extend the longevity of the nematode, at least partly by scavenging ROS.

DOI： 10.1271/bbb.110072

Language：English   Publishing type：Research paper (scientific journal)  

We investigated the effects of exposure to diazinon, an organophosphate insecticide, on the expression of antioxidant and detoxification factors in the silkmoth. Exposure to diazinon resulted in induction of mRNA encoding manganese containing superoxide dismutase (SOD) and omega-class glutathione S-transferases (GST), whereas no changes were observed in catalase, other class of GST and acetylcholinesterase. Liquid chromatography showed that the amount of reactive oxygen species was increased, whereas the amount of glutathione was decreased in the silkmoth fat body after exposure to diazinon. These results suggest that SOD and omega-class GSTs can contribute to organophosphate resistance in Lepidopterans.

DOI： 10.1111/j.1439-0418.2010.01549.x

Language：English   Publishing type：Research paper (scientific journal)  

Language：English   Publishing type：Research paper (scientific journal)  

Language：English   Publishing type：Research paper (international conference proceedings)  

Language：English   Publishing type：Research paper (international conference proceedings)  

Language：English   Publishing type：Research paper (bulletin of university, research institution)  

DOI： 10.2116/bunsekikagaku.54.391

Event date： 2007.3

Presentation type：Oral presentation (general)  

Venue：東京   Country：Japan  

Event date： 2022.11

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：大阪   Country：Japan  

シアルマリンは地下40mにある約30万年前の地層から採取される土壌であり、細胞内活性酸素消去活性を有していることが報告されている。そこで本研究では、この海洋土壌を用いて担ガンモデルマウスを使用した抗腫瘍効果を検証し、併せて悪性腫瘍罹患犬への臨床試験を行った。Colon-26 細胞移植後に各郡における腫瘍サイズの計測を行ったところシアルマリン含有量依存的な腫瘍サイズ抑制効果が確認された。臨床試験については腫瘍の縮小が明らかに認められた例、退縮効果はほとんど認められないものの延命やQOLの改善が示唆される例など一定の効果が確認された。また副作用はほとんど認められなかった。

Event date： 2022.11

Language：English   Presentation type：Oral presentation (general)  

Venue：Fukuoka   Country：Japan  

Sialmarine is a soil collected from a stratum approximately 300,000 years old at a depth of 40 m below the surface, and has been reported to have intracellular reactive oxygen species (ROS) scavenging activity. In this study, we examined the anti-tumor effect of this marine soil using mice models of carcinoma and conducted a clinical study on dogs with malignant tumors. Tumor size was measured in each group after Colon-26 cell transplantation, and a Sialmarine content-dependent tumor size suppression was overbred. In clinical trials, one case of tumor shrinkage and another case of prolongation of life and improvement of QOL were observed, although little regression was observed, indicating a certain level of efficacy. And also, almost no side effects were observed.

Methods: We used human fibrosarcoma cell line HT1080. Change of the water quality with electrolysis intensity was evaluated by pH, electrical conductivity, oxidation-reduction potential, dissolved oxygen and dissolved hydrogen. Intracellular ROS scavenging activity of ERW and H2-dissolvedwater was tested using 3’-O-Acetyl-6’-O-pentafl-uorobenzenesul-fonyl-2’,7’-difluorofluorescein（BES-H2O2）, oxygen radical absorbance capacity（ORAC）assay and the 2,2-diphenyl-1-picrylhydra-zyl （DPPH）assay. Transition of ERW compositional factor depending on electrolysis intensity was detected by ICP-MS. Observation of platinum electrode was used by scanning electron microscope (SEM).

Results and Discussion: We confirmed that ERW possessed electrolysis intensity-dependent intracellular ROS-scavenging activity and ERW exerts significantly superior ROS-scavenging activity in HT1080 cells than the equivalent level of H2-dissolved water (Fig.1). ERW retained its ROS-scavenging activity after removal of dissolved H2, but lost its activity when autoclaved (Fig.2). ORAC assay and the DPPH assay could not detect radical-scavenging activity in both ERW and H2-dissolved water. We searched for Pt from 62 elements that increased in amount in an electrolysis-dependent manner by ICP-MS analysis and grasp the surface structure appears to be a sheet of aggregated nanoparticles by using SEM (3). These results indicate that ERW contains electrolysis-dependent H2 and an additional anti-oxidative factor predicted to be platinum nanoparticles











References:
1. Sanetaka Shirahata et al., Trends in Food Science & Technology. 2012
2. Ohsawa et al., Nature medicine. 2007
3. Uscatequi AV et al., Electrochim Acta. 2013

Event date： 2022.6

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：北海道   Country：Japan  

家庭用電位治療器は頭痛・肩こり・便秘・不眠に改善効果のあると厚生労働省から認可されている交流電界発生装置である。しかしながら、PEMF治療器などの電磁波暴露の研究が広く報告されているが、電界に着目した研究は少ない。本研究では極低周波数、極低周波の条件下で交流電界の暴露が分化MC3T3E-1に与える影響を検証した。それぞれ電界強度が0.03、2.0、3.26 kV/m及び無暴露の試験区において分化MC3T3E-1の石灰化量、アルカリフォスファターゼ活性及び骨芽細胞分化関連遺伝子発現量を測定したところ電界暴露による増加が確認された。これらの結果から電磁波ではなく、電界を暴露させることで骨芽細胞の石灰化亢進が生じるという新たな知見を得ることができた。電位治療器による骨粗鬆の予防・改善につながることが期待される

Event date： 2017.11 - 2017.12

Venue：横浜  

Event date： 2017.9

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：京都   Country：Japan  

Electrochemically reduced water has hydrogen-independent intracellular reactive oxygen species scavenging activity in HT1080 cells.

○Gakuro Harada1, Kiichiro Teruya1, Shigeki Kabayama2, Takeki Hamasaki1, Sanetaka Shirahata1
1 Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University,
2 Nihon Trim Co. Ltd

[Purpose] Electrochemically reduced water (ERW) has reactive oxygen species (ROS)-scavenging activity and recent studies demonstrated that hydrogen (H2)-dissolved water exhibits ROS-scavenging activity. Thus, the antioxidative capacity of ERW is postulated to be dependent on the presence of H2 levels; however, there is no report verifying the role of dissolved H2 in ERW. In this report, we clarify whether the responsive factor for antioxidative activity in ERW is dissolved H2.
[Materials&Methods] We used human fibrosarcoma cell line HT1080. Change of the water quality with electrolysis intensity was evaluated by pH, electrical conductivity, oxidation-reduction potential, dissolved oxygen and dissolved hydrogen. Intracellular ROS
scavenging activity of ERW and H2-dissolvedwater was tested using 3’-O-Acetyl-6’-O-pentafl-
-uorobenzenesul-fonyl-2’,7’-difluorofluorescein (BES-H2O2), oxygen radical absorbance capacity (ORAC) assay and the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay. Transition of ERW compositional factor depending on electrolysis intensity was detected by ICP-MS.
[Results and Discussion] We confirm that ERW possessed electrolysis intensity-dependent intracellular ROS-scavenging activity, and ERW exerts significantly superior ROS-scavenging activity in HT1080 cells than the equivalent level of H2-dissolved water. ERW retained its ROS-scavenging activity after removal of dissolved H2, but lost its activity when autoclaved. ORAC assay and the DPPH assay could not detect radical-scavenging activity in both ERW and H2-dissolved water. These results indicate that ERW contains electrolysis-dependent H2 and an additional antioxidative factor predicted to be platinum nanoparticles, which are not radical scavengers directly, but stimulate intracellular antioxidant system(s).

Event date： 2017.3

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：京都   Country：Japan  

活性酸素(ROS)による酸化ストレスは種々の疾患の原因となり、ROSを消去することが疾患の予防や改善に繋がると考えられる。そこで我々は、疾患の予防・改善効果が報告されている電解還元水中に、極低濃度で含まれる白金ナノ粒子(PtNps)に注目した。これまでの検討から、PtNpsはROS消去活性を持つことが示されているが、PtNpsのROS消去効率はSODやCatalaseと比較して高くはなく、直接的なROS消去を期待するには、高濃度(10 ppm以上)でPtNpsを処理することが必要となる。しかし我々は、PtNpsを1 ppm以下の濃度で処理した場合にも有意な細胞内ROS消去活性を確認した。このことから、PtNpsによる細胞内のROS消去活性は直接的なものではなく、抗酸化関連遺伝子発現等を介した間接的な作用であることが推測された。そこで本研究では、低濃度のPtNpsによる細胞内でのROS消去活性の確認及びそのメカニズムの解明を目的として検討を行った。方法PtNpsの合成はヘキサクロロ白金酸塩を用い、クエン酸ナトリウムにより還元することで作製した。培養細胞は、L6細胞(ラット骨格筋細胞株)またはHT1080細胞(ヒト線維肉腫細胞株)を使用した。PtNpsの細胞内抗酸化能を確認するために、MenadioneまたはH2O2による酸化処理後にPtNpsで細胞を2時間処理した際の細胞内ROS量を測定した。また、PtNpsの2時間処理後、Menadioneで処理した際の細胞内ROS量についても測定解析を行った。次に、Menadione処理後、PtNpsを15分、30分、60分と処理し細胞内ROS量を測定した。結果・考察Menadione処理前にPtNpsを取り込ませた条件ではROS消去活性は見られず、Menadione処理後にtNpsを処理した条件ではROS消去活性が確認された。また、PtNpsの処理時間別の検討においては、30分以上処理した場合に細胞内のROS消去活性が確認された。更に白金ナノ粒子処理を施した細胞からRNAを抽出し、抗酸化関連遺伝子の発現をRT-PCRにて確認した所、複数の抗酸化関連遺伝子の発現が上昇していることが確認された。この結果から、PtNpsが細胞に取り込まれる際、細胞膜との相互作用によって抗酸化関連遺伝子の発現が増強され、細胞内の抗酸化活性が亢進されることが示唆された。

Event date： 2016.11

Language：English   Presentation type：Oral presentation (general)  

Venue：神戸   Country：Japan  

Introduction: There are many functional waters that benefit our body, such as preventing of diseases or being helpful to cure. Of these, Electrochemically reduced water（ERW）exhibits an alkaline pH, contains richly dissolved hydrogen (H2) and small amount of platinum nanoparticle, so ERW has reactive oxygen species (ROS)-scavenging activity (1). Also, recent studies demonstrated that H2-dissolved water exhibits ROS-scavenging activity (2). Thus, the anti-oxidative capacity of ERW is postulated to be dependent on the presence of H2 levels; however, there is no report verifying the role of dissolved H2 in ERW. In this report, we clarify whether the responsive factor for anti-oxidative activity in ERW is dissolved H2.

Methods: We used human fibrosarcoma cell line HT1080. Change of the water quality with electrolysis intensity was evaluated by pH, electrical conductivity, oxidation-reduction potential, dissolved oxygen and dissolved hydrogen. Intracellular ROS scavenging activity of ERW and H2-dissolvedwater was tested using 3’-O-Acetyl-6’-O-pentafl-uorobenzenesul-fonyl-2’,7’-difluorofluorescein（BES-H2O2）, oxygen radical absorbance capacity（ORAC）assay and the 2,2-diphenyl-1-picrylhydra-zyl （DPPH）assay. Transition of ERW compositional factor depending on electrolysis intensity was detected by ICP-MS. Observation of platinum electrode was used by scanning electron microscope (SEM).

Results and Discussion: We confirmed that ERW possessed electrolysis intensity-dependent intracellular ROS-scavenging activity and ERW exerts significantly superior ROS-scavenging activity in HT1080 cells than the equivalent level of H2-dissolved water (Fig.1). ERW retained its ROS-scavenging activity after removal of dissolved H2, but lost its activity when autoclaved (Fig.2). ORAC assay and the DPPH assay could not detect radical-scavenging activity in both ERW and H2-dissolved water. We searched for Pt from 62 elements that increased in amount in an electrolysis-dependent manner by ICP-MS analysis and grasp the surface structure appears to be a sheet of aggregated nanoparticles by using SEM (3). These results indicate that ERW contains electrolysis-dependent H2 and an additional anti-oxidative factor predicted to be platinum nanoparticles











References:
1. Sanetaka Shirahata et al., Trends in Food Science & Technology. 2012
2. Ohsawa et al., Nature medicine. 2007
3. Uscatequi AV et al., Electrochim Acta. 2013

Event date： 2016.11

Language：English   Presentation type：Oral presentation (general)  

Venue：神戸   Country：Japan  

1.Introduction　Electrochemically reduced water（ERW）has reactive oxygen species (ROS)-scavenging activity and recent studies demonstrated that H2-dissolved water exhibits ROS-scavenging activity. Thus, the anti-oxidative capacity of ERW is postulated to be dependent on the presence of H2 levels; however, there is no report verifying the role of dissolved H2 in ERW. In this report, we clarify whether the responsive factor for anti-oxidative activity in ERW is dissolved H2.
2.Experimental　We used human fibrosarcoma cell line HT1080. Change of the water quality with electrolysis intensity was evaluated by pH, electrical conductivity, oxidation-reduction potential, dissolved oxygen and dissolved hydrogen. Intracellular ROS scavenging activity of ERW and H2-dissolvedwater was tested using 3’-O-Acetyl-
6’-O-pentafl-uorobenzenesul-fonyl-2’,7’-difluorofluorescein（BES-H2O2）, oxygen
radical absorbance capacity（ORAC）assay and the 2,2-diphenyl-1-picrylhydra-zyl （DPPH）assay. Transition of ERW compositional factor depending on electrolysis intensity was detected by ICP-MS.
3.Results and Discussion　We confirm that ERW possessed electrolysis intensity- dependent intracellular ROS-scavenging activityand ERW exerts significantly superior ROS-scavenging activity in HT1080 cells than the equivalent level of H2-dissolved water. ERW retained its ROS-scavenging activity after removal of dissolved H2, but lost its activity when autoclaved. ORAC assay and the DPPH assay could not detect radical-scavenging activity in both ERW and H2-dissolved water.
4.Conclusion　These results indicate that ERW contains electrolysis-dependent H2 and an additional anti-oxidative factor predicted to be platinum nanoparticles.

Event date： 2016.10

Language：English   Presentation type：Oral presentation (general)  

Venue：Sofia   Country：Bulgaria  

ains richly dissolved hydrogen (H2) and small amount of platinum nanoparticles. Also, ERW has reactive oxygen species (ROS)-scavenging activity and recent studies demonstrated that H2-dissolved water exhibits ROS-scavenging activity. Thus, the antioxidative capacity of ERW is postulated to be dependent on the presence of H2 levels; however, there is no report verifying the role of dissolved H2 in ERW. In this report, we clarify whether the responsive factor for anti-oxidative activity in ERW is dissolved H2.
Experimental; We used human fibrosarcoma cell line HT1080. Change of the water quality with electrolysis intensity was evaluated by pH, electrical conductivity, oxidation-reduction potential, dissolved oxygen and H2. Intracellular ROS scavenging activity of ERW and H2-dissolved water was tested using 3’-O-Acetyl-6’-O-pentafl-uorobenzenesul-fonyl-2’,7’-difluorofluorescein (BES-H2O2), oxygen radical absorbance capacity (ORAC) assay and the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay. Transition of ERW compositional factor depending on electrolysis intensity was detected by ICP-MS.
Results and Discussion; We confirmed that ERW possessed electrolysis intensity-depen -dent intracellular ROS-scavenging activity and ERW exerts significantly superior ROS -scavenging activity in HT1080 cells than the equivalent level of H2-dissolved water. ERW retained its ROS-scavenging activity after removal of dissolved H2, but lost its activity when autoclaved. ERW contains electrolysis-dependent H2 and an additional anti-oxidative factor predicted to be platinum nanoparticles, which are not radical scavengers directly, but stimulate intracellular antioxidant system(s).

Event date： 2010.12

Presentation type：Oral presentation (general)  

Venue：大阪   Country：Japan  

Event date： 2006.11

Presentation type：Oral presentation (general)  

Venue：京都   Country：Japan  

Event date： 2005.6

Presentation type：Oral presentation (general)  

Country：United Kingdom  

Language：Japanese