Kyushu University Academic Staff Educational and Research Activities Database
Researcher information (To researchers) Need Help? How to update
Yu Hoshino Last modified date:2023.11.22

Professor / Chemistry and Biochemistry Course
Department of Applied Chemistry
Faculty of Engineering

Graduate School
Undergraduate School
Other Organization
Administration Post

E-Mail *Since the e-mail address is not displayed in Internet Explorer, please use another web browser:Google Chrome, safari.
 Reseacher Profiling Tool Kyushu University Pure
Academic Degree
Ph. D
Field of Specialization
Biomolecular Engineering, Protein Science, Polymer Science, Molecular Recognition, Colloid & Surface Chemistry
Total Priod of education and research career in the foreign country
Outline Activities
We have been developing general procedures to create synthetic nano materials, that function as antibody and enzymes, from inexpensive and stable acrylic monomers.

1, Development of Plastic Antibodies
General procedures for the creation of synthetic nanoparticles with biomacromolecular recognition sites are of significant interest as a route to stable, robust, and mass-produced substitutes for antibodies. Ideally, recognition of complex biological targets, including proteins, peptides, and carbohydrates, requires multiple functional groups that contact target molecules by a combination of electrostatic, hydrogen-bonding, van der Waals, and/or hydrophobic interactions. We have shown that copolymerization of optimized combination and ratio of functional acrylic-monomers creates synthetic polymer materials with molecular recognition sites for peptides and proteins. In addition to optimizing functional complementarity, the affinity sites can be further enhanced by molecular imprinting and/or affinity purification process. Those particles are capable of recognizing, neutralizing, and clearing toxic peptide and proteins even in the bloodstream of living animals. However, in contrast to antibodies whose exact sequence can be determined and cloned, polymerized materials result in heterogeneous structures with a distribution of recognition sites. Our goal is to achieve general strategy to create plastic antibodies with uniform molecular structure as "monoclonal plastic antibodies".

2, Development of Plastic Enzymes
Life is a dissipate structure appears in the non-equilibrium open system. To maintain the structure, enzymes mediates most reactions in the body including molecular transportation, energy conversion and molecular conversion that are necessarily for the life. To keep the life alive, it is important for the each enzyme to recognize and bind to specific molecules and ions and catalyze specific reaction just in time. It is also very important to control binding and dissociating kinetics to keep the dissipate structure.
One of the challenges for us is to construct synthetic molecular system like living life. So far, we found that target binding and dissociation kinetics of nanoparticles can be tuned by conformation change and polymer density of the particles. Binding constant to target proteins and ions can be reversibly switched on/off by temperature-induced phase transition of the polymer chains. By applying the fundamental findings, we are trying to develop inexpensive and scalable chemical process for the conversion of low temperature waste heat in to reusable chemical potential.

(1) PNAS 109 (1), 33-38, 2012
(2) JACS 134, 15765-15772, 2012
(3) JACS 130, 15242-15243, 2008
(4) JACS 132, 13648-13650, 2010
(5) JACS 136, 1194-1197, 2014
(6) JACS 132, 6644-6645, 2010
(7) JACS 137, 10878-10881, 2015
(8) Angew. Chem. Intl Ed. 51, 2405-2408, 2012
(9) JACS 134, 15209-15212, 2012
(10) Biomacromolecules 15, 541–547, 2014
(11) JACS 138, 4282-4285, 2016
(12) Biomacromolecules,16, 411–421, 2015
(13) Advanced Materials 26, 2449–2606, 2014
(14) JACS 134, 18177-18180, 2012
(15) Chem. Sci. 6, 6112, 2015
(16) Angew. Chem. Intl Ed. 53, 2654–2657, 2014
Research Interests
  • Preparation of homogeneous oligomer library and development of their bio-application
    keyword : homogeneous oligomer
  • Development of CO2 Separation Membranes
    keyword : CO2
  • Development of protein separation media
    keyword : Synthetic Polymer, Moleculr Recognition, Antibody
  • Development of Materials for Heat Energy Transduction
    keyword : Heat Energy Transduction
  • Development of Energy Efficient CO2 Adsorbent
    keyword : CO2
  • Development of Plastic Antibodies
    keyword : Synthetic Polymer, Moleculr Recognition, Antibody
Academic Activities
1. Yusuke Saito, Ryutaro Honda, Sotaro Akashi, Hinata Takimoto, Masanori Nagao, Yoshiko Miura, Yu Hoshino, Polymer Nanoparticles with Uniform Monomer Sequences for Sequence Specific Peptide Recognition, Angew. Chem. Int. Ed, 10.1002/ange.202206456, e202206456, 2022.05, Synthetic polymer nanoparticles (NPs) that recognize and neutralize target biomacromolecules are of considerable interest as “plastic antibodies”, synthetic mimics of antibodies. However, monomer sequences in the synthetic NPs are heterogeneous. The heterogeneity limits the target specificity and safety of the NPs. Herein, we report the synthesis of NPs with uniform monomer sequences for recognition and neutralization of target peptides. A multifunctional oligomer with a precise monomer sequence that recognizes the target peptide was prepared via cycles of reversible addition–fragmentation chain transfer (RAFT) polymerization and flash chromatography. The oligomer or blend of oligomers was used as a chain transfer agent and introduced into poly(N-isopropyl acrylamide) hydrogel NPs by radical polymerization. Evaluation of the interaction with the peptides revealed that multiple oligomers in NPs cooperatively recognized the sequence of the target peptide and neutralized its toxicity. Effect of sequence, combination, density and molecular weight distribution of precision oligomers on the affinity to the peptides was also investigated..
2. Yusuke Yonamine, Takuya Asai, Yuta Suzuki, Takuro Ito, Yasuyuki Ozeki, Yu Hoshino, Probing the biogenesis of polysaccharide granules in algal cells at sub-organellar resolution via Raman microscopy with stable isotope labeling, Analytical Chemistry, 10.1021/acs.analchem.1c03216, 93, 16796, 2021.12.
3. Yu Hoshino, Tomohiro Gyobu, Kazushi Imamura, Akira Hamasaki, Ryutaro Honda, Ryoga Horii, Chie Yamashita, Yuki Terayama, Takeshi Watanabe, Shoma Aki, Yida Liu, Junko Matsuda, Yoshiko Miura, Ikuo Taniguchi, Assembly of Defect-Free Microgel Nanomembranes for CO2 Separation, ACS Applied Bio Materials, 3, 6, 2021.07.
4. Benshuai Guo, Yoshiko Miura, Yu Hoshino, Rational design of thermocells driven by the volume phase transition of hydrogel nanoparticles, ACS Applied Materials & Interfaces, 10.1021/acsami.1c07266, 13, 32184, 2021.07.
5. Ryutaro Honda, Akira Hamasaki, Yoshiko Miura, Yu Hoshino, Thermoresponsive CO 2 absorbent for various CO 2 concentrations: tuning the p K a of ammonium ions for effective carbon capture, Polymer Journal, 10.1038/s41428-020-00407-5, 53, 157-167, 2021.01.
6. Benshuai Guo, Yu Hoshino, Fan Gao, Keisuke Hayashi, Yoshiko Miura, Nobuo Kimizuka, Teppei Yamada, Thermocells driven by phase transition of hydrogel nanoparticles, J. Am. Chem Soc,, 142, 41, 17318-17322, 2020.09, Thermoelectric conversion of low temperature, delocalized, and abundant thermal sources is crucial for the development of the Internet of Things (IoT) and/or a carbon-free society. Thermocells are of great interest in thermoelectric conversion of low-temperature heat due to the low cost and flexibility of components. However, significant improvement of the conversion efficiency is required for the practical use of the cells. Here, we report thermo-electrochemical cells driven by volume phase transition (VPT) of hydrogel nanoparticles (NPs). Entropically driven VPT of poly(N-isopropylacrylamide) NPs containing carboxylic acids and amines generates a pH gradient of up to 0.049 and −0.053 pH K–1, respectively, around physiological temperature. The pH gradient triggers the proton-coupled electron transfer (PCET) reactions of quinhydrone on the electrodes, resulting in the highly efficient thermoelectric conversion with a Seebeck coefficient (Se) of −6.7 and +6.1 mV K–1. Thermocells driven by phase transition of hydrogels provide a nontoxic, flexible, and inexpensive charger that harvests carbon-free energy from abundant energy sources such as solar, body and waste heat..
7. Hiroyuki Koide, Anna Okishima, Yu Hoshino, Yuri Kamon, Keiichi Yoshimatsu, Kazuhiro Saito, Ikumi Yamauchi, Saki Ariizumi, Yuqi Zhou, Ting-Hui Xiao, Keisuke Goda, Naoto Oku, Tomohiro Asai, Kenneth J Shea, Synthetic hydrogel nanoparticles for sepsis therapy, Nature communications, 10.1038/s41467-021-25847-2, 12, 5552, 2020.09, Sepsis is a life-threatening condition caused by the extreme release of inflammatory mediators into the blood in response to infection (e.g., bacterial infection, COVID-19), resulting in the dysfunction of multiple organs. Currently, there is no direct treatment for sepsis. Here we report an abiotic hydrogel nanoparticle (HNP) as a potential therapeutic agent for late-stage sepsis. The HNP captures and neutralizes all variants of histones, a major inflammatory mediator released during sepsis. The highly optimized HNP has high capacity and long-term circulation capability for the selective sequestration and neutralization of histones. Intravenous injection of the HNP protects mice against a lethal dose of histones through the inhibition of platelet aggregation and migration into the lungs. In vivo administration in murine sepsis model mice results in near complete survival. These results establish the potential for synthetic, nonbiological polymer hydrogel sequestrants as a new intervention strategy for sepsis therapy and adds to our understanding of the importance of histones to this condition..
8. Yu Hoshino, Shinnosuke Shimohara, Yusuke Wada, Masahiko Nakamoto, Yoshiko Miura, Affinity Purification of Multifunctional Oligomeric Ligands Synthesizedvia Controlled Radical Polymerization, J Mat. Chem B,, 8, 26, 5597-5601, 2020.07.
9. Nao Nitta, Takanori Iino, Akihiro Isozaki, Mai Yamagishi, Yasutaka Kitahama, Shinya Sakuma, Yuta Suzuki, Hiroshi Tezuka, Minoru Oikawa, Fumihito Arai, Takuya Asai, Dinghuan Deng, Hideya Fukuzawa, Misa Hase, Tomohisa Hasunuma, Takeshi Hayakawa, Kei Hiraki, Kotaro Hiramatsu, Yu Hoshino, Mary Inaba, Yuki Inoue, Takuro Ito, Masataka Kajikawa, Hiroshi Karakawa, Yusuke Kasai, Yuichi Kato, Hirofumi Kobayashi, Cheng Lei, Satoshi Matsusaka, Hideharu Mikami, Atsuhiro Nakagawa, Keiji Numata, Tadataka Ota, Takeichiro Sekiya, Kiyotaka Shiba, Yoshitaka Shirasaki, Nobutake Suzuki, Shunji Tanaka, Shunnosuke Ueno, Hiroshi Watarai, Takashi Yamano, Masayuki Yazawa, Yusuke Yonamine, Dino Di Carlo, Yoichiroh Hosokawa, Sotaro Uemura, Takeaki Sugimura, Yasuyuki Ozeki, Keisuke Goda, Raman image-activated cell sorting, Nature communications, 10.1038/s41467-020-17285-3, 11, 3452, 2020.07.
10. Hinata Takimoto, Sho Katakami, Yoshiko Miura, Yu Hoshino, Controlling the block sequence of multi-block oligomer ligands for neutralizationof a target peptide, Materials Advances,, 4, 604-608, 2020.06.
11. Ryutaro Honda,Tomohiro Gyobu, Hideo Shimahara, Yoshiko Miura, Yu Hoshino, Electrostatic Interactions Between Acid-/Base-Containing Polymer Nanoparticles and Proteins: Impact of Polymerization pH, ACS Applied Bio Materials,, 3, 6, 3827-3834, 2020.05.
12. Akihiro Isozaki, Hideharu Mikami, Hiroshi Tezuka, Hiroki Matsumura, Kangrui Huang, Marino Akamine, Kotaro Hiramatsu, Takanori Iino, Takuro Ito, Hiroshi Karakawa, Yusuke Kasai, Yan Li, Yuta Nakagawa, Shinsuke Ohnuki, Tadataka Ota, Yong Qian, Shinya Sakuma, Takeichiro Sekiya, Yoshitaka Shirasaki, Nobutake Suzuki, Ehsen Tayyabi, Tsubasa Wakamiya, Muzhen Xu, Mai Yamagishi, Haochen Yan, Qiang Yu, Sheng Yan, Dan Yuan, Wei Zhang, Yaqi Zhao, Fumihito Arai, Robert E Campbell, Christophe Danelon, Dino Di Carlo, Kei Hiraki, Yu Hoshino, Yoichiroh Hosokawa, Mary Inaba, Atsuhiro Nakagawa, Yoshikazu Ohya, Minoru Oikawa, Sotaro Uemura, Yasuyuki Ozeki, Takeaki Sugimura, Nao Nitta, Keisuke Goda, Intelligent image-activated cell sorting 2.0, Lab on a Chip, 10.1039/D0LC00080A , 20, 2273, 2020.05.
13. Yusuke Yonamine, Kotaro Hiramatsu, Takuro Ideguchi, Takuro Ito, Tomomi FujiwaraYoshiko Miura, Keisuke Goda, Yu Hoshino, Spatiotemporal monitoring of intracellular metabolic dynamics by resonanceRaman microscopy with isotope labeling, RSC Advances,, 10, 16679-16686, 2020.04.
14. Yu Hoshino,Mitsunori Moribe, Naoki Gondo, Toshiki Jibiki, Masahiko Nakamoto, BenshuaiGuo, Rinoka Adachi, Yoshiko Miura, Combining Acid- and Base-Imprinted Nanoparticles in a Hydrogel Film forTemperature-Responsive Quick and Reversible Capture of Salt, ACS Appl. Polym. Mater,, 2, 505-514, 2020.02.
15. Yu Hoshino, Shohei Taniguchi,Hinata Takimoto, Sotaro Akashi, Sho Katakami, Yusuke Yonamine, Yoshiko Miura, Homogeneous Oligomeric Ligands Prepared via Radical Polymerization thatRecognize and Neutralize a Target Peptide, Angew. Chem. Int. Ed,, 132, 689-693, 2020.02, Abiotic ligands that bind to specific biomolecules have attracted attention as substitutes for biomolecular ligands, such as antibodies and aptamers. Radical polymerization enables the production of robust polymeric ligands from inexpensive functional monomers. However, little has been reported about the production of monodispersed polymeric ligands. Herein, we present homogeneous ligands prepared via radical polymerization that recognize epitope sequences on a target peptide and neutralize the toxicity of the peptide. Taking advantage of controlled radical polymerization and separation, a library of multifunctional oligomers with discrete numbers of functional groups was prepared. Affinity screening revealed that the sequence specificity of the oligomer ligands strongly depended on the number of functional groups. The process reported here will become a general step for the development of abiotic ligands that recognize specific peptide sequences..
16. Yida Liu, Takashi Kodama, Taisuke Kojima, Ikuo Taniguchi, Hirokazu Seto, Yoshiko Miura, Yu Hoshino, Fine-tuning of the surface porosity of micropatterned polyethersulfonemembranes prepared by phase separation micromolding, Polymer J,, 52, 397-403, 2020.01.
17. Nobutoshi Ota, Yusuke Yonamine, Takuya Asai, Yaxiaer Yalikun, Takuro Ito, Yasuyuki Ozeki, Yu Hoshino, Yo Tanaka, Isolating Single Euglena gracilis Cells by Glass Microfluidics for Raman Analysis of Paramylon Biogenesis, Analytical chemistry, 10.1021/acs.analchem.9b01007, 91, 9631, 2019.07.
18. Anna Okishima, Hiroyuki Koide, Yu Hoshino, Hiromichi Egami, Yoshitaka Hamashima, Naoto Oku, Tomohiro Asai, Design of Synthetic Polymer Nanoparticles Specifically Capturing Indole, a Small Toxic Molecule, Biomacromolecules, 10.1021/acs.biomac.8b01820, 20, 4, 1644-1654, 2019.04.
19. Masanori Nagao, Teruhiko Matsubara,Yu Hoshino, Toshinori Sato, and Yoshiko Miura, Topological Design of Star Glycopolymers for Controlling the Interactionwith the Influenza Virus, Bioconjugate Chemistry, 10.1021/acs.bioconjchem.9b00134, 30, 1192-1198, 2019.03.
20. Hiroyuki Koide, Keiichi Yoshimatsu, Yu Hoshino, Saki Ariizumi, Anna Okishima, Takafumi Ide, Hiromichi Egami, Yoshitaka Hamashima, Yuri Nishimura, Hiroaki Kanazawa, Yoshiko Miura, Tomohiro Asai, Naoto Oku, Kenneth J. Shea, Sequestering and inhibiting a vascular endothelial growth factor in vivo by systemic administration of a synthetic polymer nanoparticle, Journal of Controlled Release, 10.1016/j.jconrel.2018.12.033, 295, 13-20, 2019.02.
21. Koide, H. Yoshimatsu, K. Hoshino, Y. Ariizumi, S. Okishima,A. Ide, T. Egami, H. Hamashima, Y. Nishimura, Y. Kanazawa, H. Miura, Y.Asai, T. Oku, N. Shea, K.J., Sequestering and inhibiting a vascular endothelial growth factorin vivo by systemic administration of a synthetic polymer nanoparticle , Journal of Controlled Release, Doi: 10.1016/j.jconrel.2018.12.033, 295, 13-20, 2019.02, [URL].
22. Kotaro Hiramatsu, Takuro Ideguchi, Yusuke Yonamine, Sang Wook Lee, Yizhi Luo, Kazuki Hashimoto, Takuro Ito, Misa Hase, Jee Woong Park, Yusuke Kasai, Shinya Sakuma, Takeshi Hayakawa, Fumihito Arai, Yu Hoshino, Keisuke Goda, High-throughput label-free molecular fingerprinting flow cytometry, Science Advances, 10.1126/sciadv.aau0241, 5, 1, 2019.01.
23. Nao Nitta, Takeaki Sugimura, Akihiro Isozaki, Hideharu Mikami, Kei Hiraki, Shinya Sakuma, Takanori Iino, Fumihito Arai, Taichiro Endo, Yasuhiro Fujiwaki, Hideya Fukuzawa, Misa Hase, Takeshi Hayakawa, Kotaro Hiramatsu, Yu Hoshino, Mary Inaba, Takuro Ito, Hiroshi Karakawa, Yusuke Kasai, Kenichi Koizumi, Sang Wook Lee, Cheng Lei, Ming Li, Takanori Maeno, Satoshi Matsusaka, Daichi Murakami, Atsuhiro Nakagawa, Yusuke Oguchi, Minoru Oikawa, Tadataka Ota, Kiyotaka Shiba, Hirofumi Shintaku, Yoshitaka Shirasaki, Kanako Suga, Yuta Suzuki, Nobutake Suzuki, Yo Tanaka, Hiroshi Tezuka, Chihana Toyokawa, Yaxiaer Yalikun, Makoto Yamada, Mai Yamagishi, Takashi Yamano, Atsushi Yasumoto, Yutaka Yatomi, Masayuki Yazawa, Dino Di Carlo, Yoichiroh Hosokawa, Sotaro Uemura, Yasuyuki Ozeki, Keisuke Goda, Intelligent Image-Activated Cell Sorting, Cell, 10.1016/j.cell.2018.08.028, 175, 1, 266-276.e13, 2018.09, A fundamental challenge of biology is to understand the vast heterogeneity of cells, particularly how cellular composition, structure, and morphology are linked to cellular physiology. Unfortunately, conventional technologies are limited in uncovering these relations. We present a machine-intelligence technology based on a radically different architecture that realizes real-time image-based intelligent cell sorting at an unprecedented rate. This technology, which we refer to as intelligent image-activated cell sorting, integrates high-throughput cell microscopy, focusing, and sorting on a hybrid software-hardware data-management infrastructure, enabling real-time automated operation for data acquisition, data processing, decision-making, and actuation. We use it to demonstrate real-time sorting of microalgal and blood cells based on intracellular protein localization and cell-cell interaction from large heterogeneous populations for studying photosynthesis and
atherothrombosis, respectively. The technology is highly versatile and expected to enable machinebased scientific discovery in biological, pharmaceutical, and medical sciences..
24. Yu Hoshino, Toshiki Jibiki, Masahiko Nakamoto, Yoshiko Miura, Reversible p Ka Modulation of Carboxylic Acids in Temperature-Responsive Nanoparticles through Imprinted Electrostatic Interactions, ACS Applied Materials and Interfaces, 10.1021/acsami.8b11397, 10, 37, 31096-31105, 2018.09.
25. Akihiro Isozaki, Hideharu Mikami, Kotaro Hiramatsu, Shinya Sakuma, Yusuke Kasai, Takanori Iino, Takashi Yamano, Atsushi Yasumoto, Yusuke Oguchi, Nobutake Suzuki, Yoshitaka Shirasaki, Taichiro Endo, Takuro Ito, Kei Hiraki, Makoto Yamada, Satoshi Matsusaka, Takeshi Hayakawa, Hideya Fukuzawa, Yutaka Yatomi, Fumihito Arai, Dino Di Carlo, Atsuhiro Nakagawa, Yu Hoshino, Yoichiroh Hosokawa, Sotaro Uemura, Takeaki Sugimura, Yasuyuki Ozeki, Nao Nitta, Keisuke Goda, A practical guide to intelligent image-activated cell sorting, Nature protocols, 10.1038/s41596-019-0183-1, 14, 2370, 2018.08.
26. Hiroyuki Koide, Hiroki Tsuchida, Masahiko Nakamoto, Anna Okishima, Saki Ariizumi, Chiaki Kiyokawa, Tomohiro Asai, Yu Hoshino, Naoto Oku, Rational designing of an antidote nanoparticle decorated with abiotic polymer ligands for capturing and neutralizing target toxins, Journal of Controlled Release, 10.1016/j.jconrel.2017.10.028, 268, 335-342, 2017.12.
27. Yusuke Yonamine, Yuta Suzuki, Takuro Ito, Yoshiko Miura, Keisuke Goda, Yasuyuki Ozeki, Yu Hoshino, Monitoring Photosynthetic Activity in Microalgal Cells by Raman Spectroscopy with Deuterium Oxide as a Tracking Probe, ChemBioChem, 10.1002/cbic.201700314, 18, 20, 2063-2068, 2017.10.
28. H. Koide, K. Yoshimatsu, Y. Hoshino, S.-H. Lee, A. Okajima, S. Ariizumi, Y. Narita, Y. Yonamine, A. C. Weisman, Y. Nishimura, N. Oku, Y. Miura, K. J. Shea, A polymer nanoparticle with engineered affinity for a vascular endothelial growth factor (VEGF165), Nat. Chem., 10.1038/nchem.2749, 2017.03.
29. M. Yue, K. Imai, C. Yamashita, Y. Miura, Y. Hoshino, Effects of Hydrophobic Modifications and Phase Transitions of Polyvinylamine Hydrogel Films on Reversible CO2 Capture Behavior: Comparison between Copolymer Films and Blend Films for Temperature‐Responsive CO2 Absorption, Macromol. Chem. and Phys., 10.1002/macp.201600570, 218, 1600570, 2017.02.
30. Yu Hoshino, Takaaki Miyoshi, Masahiko Nakamoto, Yoshiko Miura, Wide-range p
K a tuning of proton imprinted nanoparticles for reversible protonation of target molecules via thermal stimuli, Journal of Materials Chemistry B, 10.1039/c7tb02107k, 5, 46, 9204-9210, 2017.01.
31. Masahiko Nakamoto, Tadashi Nonaka, Yoshiko Miura, Kenneth J. Shea, Yu Hoshino*, Design of Synthetic Polymer Nanoparticles that Facilitate Resolubilization and Refolding of Aggregated Positively Charged Lysozyme, J. Am. Chem. Soc., 10.1021/jacs.5b12600, 138, 2016.02, [URL].
32. Lee Haejoo, Yu Hoshino*, Yusuke Wada, Yuka Arata, Atsushi Maruyama, Yoshiko Miura, Minimization of synthetic polymer ligands for specific recognition and neutralization of a toxic peptide, J. Am. Chem. Soc., 10.1021/jacs.5b05259, 137, 10878-10881, 2015.09, [URL].
33. Mengchen Yue, Yu Hoshino*, Yoshiko Miura, Design Rationale of Thermally Responsive Microgel Particle Films That Reversibly Absorb Large Amounts of CO2: Fine Tuning the pKa of Ammonium Ions in the Particles, Chem. Sci., 10.1039/C5SC01978H, ASAP, 2015.08, [URL].
34. Keiichi Yoshimatsu, Hiroyuki Koide, Yu Hoshino, Kenneth J. Shea, Preparation of abiotic polymer nanoparticles for sequestration and neutralization of a target peptide toxin, Nature protocols, 10.1038/nprot.2015.032, 10, 595–604, 2015.04, [URL].
35. Yu Hoshino*, Yuka Arata, Haejoo Lee, Yusuke Yonamine, Shih-Hui Lee, Aki Yamasaki, Ryousuke Tsuhara, Katsuhiko Yano, Kenneth J Shea, Yoshiko Miura, Preparation of nanogel-immobilized porous gel beads for affinity separation of proteins: fusion of nano and micro gel materials, Polymer Journal, 10.1038/pj.2014.101, 47, 220–225, 2015.01, [URL].
36. Yusuke Wada, Haejoo Lee, Yu Hoshino*, Shunsuke Kotani, Kenneth J. Shea, Yoshiko Miura, Design of multi-functional linear polymers that capture and neutralize a toxic peptide: a comparison with cross-linked nanoparticles, Journal of Materials Chemistry B, 10.1039/C4TB01967A, 3, 1706-1711, 2015.01, [URL].
37. Yoke-Ming Wong, Yu Hoshino*, Kumar Sudesh, Yoshiko Miura, Keiji Numata*, Optimization of poly (N-isopropylacrylamide) as an artificial amidase, Biomacromolecules, 10.1021/bm501671r, 16, 411–421, 2015.01, [URL].
38. Adam Weisman, Yingyao Allie Chen, Yu Hoshino, Huiting Zhang, Kenneth J. Shea, Engineering Nanoparticle Antitoxins Utilizing Aromatic Interactions, Biomacromolecules, 10.1021/bm500666j, 15, 3290–3295, 2014.06, [URL].
39. Yu Hoshino*, Ryohei C. Ohashi, Yoshiko Miura, Rational Design of Synthetic Nanoparticles with a Large Reversible Shift of Acid Dissociation Constants: Proton Imprinting in Stimuli Responsive Nanogel Particles, Advanced Mater, 10.1002/adma.201305957, 26, 2449–2606, 2014.03, [URL].
40. K. Yoshimatsu, T. Yamazaki, Yu Hoshino, L. F. Epstein, L. P. Miranda, P. Tagari, J. M. Beierle, Y. Ynamine, K. J. Shea, Epitope Discovery for a Synthetic Polymer Nanoparticle: A New Strategy for Developing a Peptide Tag, J. Am. Chem. Soc., 10.1021/ja410817p, 136, 1194–1197, 2014.01, [URL].
41. Mengchen Yue, Yu Hoshino*, Yukinori Ohshiro, Kazushi Imamura, Yoshiko Miura, Temperature-Responsive Microgel Films as Reversible Carbon Dioxide Absorbents in Wet Environment, Angew. Chem. Int. Ed., 10.1002/ange.201309758, 126, 2692–2695, 2014.01, [URL].
42. Masahiko Nakamoto, Yu Hoshino*, Yoshiko Miura*, Effect of Physical Properties of Nanogel Particles on the Kinetic Constants of Multipoint Protein Recognition Process, Biomacromolecules, 10.1021/bm401536v, 15, 541–547, 2013.12, [URL].
43. Yonamine, Yusuke, Yoshimatsu, Keiichi, Lee, Shih-Hui, Yu Hoshino, Okahata, Yoshio, Shea, Kenneth J, Polymer Nanoparticle-Protein Interface. Evaluation of the Contribution of Positively Charged Functional Groups to Protein Affinity, ACS APPLIED MATERIALS & INTERFACES, 10.1021/am302404q, 5, 2, 374-379, 2013.01.
44. Y. Hoshino*, K. Imamura , M. Yue , G. Inoue , Y. Miura*, Reversible absorption of CO2 triggered by phase transition of amine-containing micro- and nano-gel particles, J. Am. Chem. Soc., 10.1021/ja3080192, 134, 18177-18180, 2012.10, [URL].
45. Y. Hoshino*, M. Nakamoto, and Y. Miura*, Control of protein-binding kinetics on synthetic polymer nanoparticles by tuning flexibility and inducing conformation changes of polymer chains, J. Am. Chem. Soc., 10.1021/ja306053s, 134, 15209−15212, 2012.09, [URL].
46. S.-H. Lee, Y. Hoshino, A. Randall, Z. Zeng, P. Baldi, R.-a. Doong and K. J. Shea, Engineered Synthetic Polymer Nanoparticles as IgG Affinity Ligands, J. Am. Chem. Soc., 10.1021/ja303612d, 134, 15765−15772, 2012.09, [URL].
47. Y. Yonamine, Y. Hoshino, and K. J. Shea, An ELISA-mimic screen for synthetic polymer nanoparticles with high affinity to target proteins, Biomacromolecules, 10.1021/bm300986j, 13, 2952–2957, 2012.07, [URL].
48. K. Yoshimatsu, B. K. Lesel, Y. Yonamine, J. M. Beierle, Y. Hoshino, and K. J. Shea, Temperature-Responsive “Catch and Release” of Proteins by using Multifunctional Polymer-Based Nanoparticles, Angew. Chem. Int. Ed., 10.1002/anie.201107797, 51, 2405–2408, 2012.03, [URL].
49. Y. Hoshino*, H. Koide, K. Furuya, W. W. Haberaecker III, S. Lee, T. Kodama, H. Kanazawa, N. Oku, and K. J. Shea*, The Rational Design of a Synthetic Polymer Nanoparticles that Neutralizes a Toxic Peptide in Vivo, Proc. Natl. Acad. Sci. USA, 10.1073/pnas.1112828109, 109, 33-38, 2012.01, [URL].
50. Y. Hoshino, and K. J. Shea*, Evolution of Plastic Antibodies, J. Mat. Chem., 2010.10, [URL].
51. Y. Hoshino*, W. W. Haberaecker III, T. Kodama, Z. Zeng, Y. Okahata, and K. J. Shea*, Affinity Purification of Multifunctional Polymer Nanoparticles, J. Am. Chem. Soc., 10.1021/ja1058982, 132, 13648-13650, 2010.09, [URL].
52. Y. Hoshino*, H. Koide, T. Urakami, H. Kanazawa, T. Kodama, N. Oku, and K. J. Shea*, Recognition, Neutralization, and Clearance of Target Peptides in the Bloodstream of Living Mice by Molecularly Imprinted Polymer Nanoparticles: A Plastic Antibody, J. Am. Chem. Soc., 10.1021/ja102148f, 132, 6644–6645, 2010.04, [URL].
53. Z. Zeng, Y. Hoshino, A. Rodoriguez, H. Yoo, and K. J. Shea, Synthetic Polymer Nanoparticles with Antibody-Like Affinity for a Hydrophilic Peptide, ACS nano, 10.1021/nn901256s, 4, 199–204, 2009.12, [URL].
54. Y. Hoshino*, T. Urakami, T. Kodama, H. Koide, N. Oku, Y. Okahata, and K. J. Shea*, Design of Synthetic Polymer Nanoparticles that Capture and Neutralize Toxic Peptide, Small, 10.1002/smll.200900186, 5, 1562–1568, 2009.03.
55. Y. Hoshino, T. Kodama, Y. Okahata, and K. J. Shea, Peptide Imprinted Polymer nanoparticles “Plastic Antibodies”, J. Am. Chem. Soc., 10.1021/ja8062875, 130, 15242-15243, 2008.10, [URL].
56. Y. Hoshino, T. Kawasaki, and Y. Okahata, Effect of Ultrasound on DNA Polymerase Reactions: Monitoring on a 27-MHz Quartz Crystal Microbalance, Biomacromolecules, 10.1021/bm050738e, 7, 682–685, 2006.02, [URL].
57. Y. Hoshino, S. Tajima, H. Nakayama, and Y. Okahata, A RNA-aligned Film Prepared from a RNA-Lipid Complex, Macromol. Rapid Commun., 10.1002/1521-3927(20020301)23:4, 23, 253-255, 2002.03, [URL].
1. Yu Hoshino1, Preparation of Defectless Hydrogel Nanomembranes for CO2 Separation by Microgel Particles, 2019 MRS SPRING MEETING & EXHIBIT, 2019.04.
2. 郭本帥、星野友、山田鉄兵、三浦佳子, Thermo-Electrochemical Cell Development by Using Temperature Responsive Nanogel, 第67回高分子学会年次大会, 2018.05.
3. Y. Hoshino, Tuning pKa of Brønsted Acids in Temperature-Responsive Hydrogel Particles by Proton- and Ion-Imprinting Strategy, ACS 255rd National Meeting 2018, 2018.03.
4. Shohei Taniguchi, Sho Katakami, Yusuke Yonamine, Yu Hoshino, Yoshiko Miura, Effects of the Number of Functional Groups of Discrete Oligomer Ligands Affinity to Target Peptide, 2017 Kyushu-Seibu/Pusan-Gyeongnam Joint Symposium on High Polymers (18th) and Fibers (16th), 2017.12.
5. Ryuitaro Honda, Tomohiro Gyobu, Hideto Shimahara [JAIST], Yoshiko Miura, Yu Hoshino, Effect of Composition of Gel Particles on the Immobilization of Carbonic Anhydrase, 2017 Kyushu-Seibu/Pusan-Gyeongnam Joint Symposium on High Polymers (18th) and Fibers (16th), 2017.12.
6. Mitsunori Moribe, Naoki Gondo, Masahiko Nakamoto, Yu Hoshino, Yoshiko Miura, Provement of Reversible Salt Absorption Efficiency of Temperature Responsive Gel Particle Composite Membrane, 2017 Kyushu-Seibu/Pusan-Gyeongnam Joint Symposium on High Polymers (18th) and Fibers (16th), 2017.12.
7. Y. Hoshino, T. Miyoshi, T. Jibiki, Y. Miura, Tuning pKa of Brønsted Acids in Stimuli Responsive Nanogel Particles by Proton- and Ion-Imprinting Strategy for Reversible Capture of Target Molecules, Affinity 2017, 2017.06, Synthetic materials that alter their binding affinity to target molecules in response to external stimuli have gained considerable attention as substitutes for protein based ligands. Recently, we revealed that pNIPAm-based NPs that show large and reversible pKa shifts can be prepared by the “proton imprinting” and “microenvironment imprinting” strategy. The pKa variation range of carboxylic acids in the NPs can further be tuned by designing structure of monomers containing Brønsted acids and tuning cross-linking density and size of NPs. The pKa variation range can be lowered/raised to be 4-9 by stabilizing/destabilizing carboxylate anions by modifying the acids with electron withdrawing/donating group. The pKa of acids can also be lowered dramatically by imprinting cationic functional group such as guanidium group around the carboxylate anions.
Our results provide a guide for designing stable and inexpensive materials for many biological and chemical applications as temperature-dependent affinity media and pH modifiers..
8. Yu Hoshino, Development of Protein-Mimic Nanoparticles for Effective CO2 Separation, 23rd iCeMS International Symposium, 2017.05.
9. Y. Hoshino, T. Gyobu, R. Honda, K. Imamura, C. Yamashita, T. Watanabe, I. Taniguchi, Y. Miura, Development of Nanogel Membranes for CO2 Separation, ACS 253rd National Meeting 2017, 2017.04.
10. Yu Hoshino, Toshiki Jibiki, Takaaki Miyoshi, Masaaki Nakamoto Yoshiko Miura, Tuning pKa Value of Bronsted Acids in Hydrogel Nanoparticles by Ion Imprinting Strategy, Gelsympo 2017, 2017.03.
11. Yu Hoshino, Design of Synthetic Polymer Nanoparticles that Function as Molecular Chaperones, Asian International Symposium-Natural Products Chemistry, Chemical Biology/Biofunctional Chemistry and Biotechnology, 2017.03.
12. 星野 友, 大橋 良平, 三浦 佳子, Development of Enzyme-Mimic Proton Transfer Systems, IUMRS-International Conference on Electronic Materials (IUMRS-ICEM 2014), 2014.08.
13. 星野 友, 大橋 良平, 三浦 佳子, Preparation of Temperature Responsive Nanogels with Carboxylic Acids which Undergo Large and Reversible pKa Shift, IUMRS-International Conference on Electronic Materials (IUMRS-ICEM 2014), 2014.08.
14. 星野 友, 大橋良平, 三浦 佳子, Preparation of Proton Imprinted Nanoparticles with Switchable pKa Values: Toward Plastic Enzymes, MRS Fall Meeting,2013, 2013.12.
15. Yu Hoshino, Kazushi Imamura, Mengchen Yue, Yoshiko Miura, Reversible absorption of CO2 by aqueous solution of amine-containing poly-N-isopropylacrylamide particles, The 244th ACS National Meeting , 2013.04.
16. Ryohei Ohashi, Yu Hoshino, Yoshiko Miura, Reversible absorption of protons by temperature-responsive gel-particles, The 244th ACS National Meeting , 2013.04.
17. Yu Hoshino, Mengchen Yue, Yoshiko Miura, Hydrogel films consisting of temperature-responsive nanogels as an absorbent to capture CO2 reversibly, The 244th ACS National Meeting , 2013.04.
18. LEE HAEJOO, Yusuke Wada, Yu Hoshino, Yoshiko Miura, Preparation and characterization synthetic polymer ligands that recognize target peptides with multipoint interaction, The 244th ACS National Meeting , 2013.04.
19. LEE HAEJOO, Yusuke Wada, Yu Hoshino, Yoshiko Miura, Minimization of Polymer Ligands that Interact with Targeted Peptide, Soft-interfaces Mini-symposium 2013-Physical Chemistry and Characterization of Soft-interfaces-(SIMS2013) , 2013.03.
20. Mengchen Yue, Yu Hoshino, Yoshiko Miura, Study on reversible CO2 absorption by thermo-responsive hydrogel films, Soft-interfaces Mini-symposium 2013-Physical Chemistry and Characterization of Soft-interfaces-(SIMS2013) , 2013.03.
Membership in Academic Society
  • The Society of Polymer Science, Japan
  • The Chemical Society of Japan
  • Materials Research Society
  • American Chemical Society
  • The Chemical Society of Japan
  • The Electrochemical Society of Japan
  • The Society of Chemical Engineers
  • The Society of Polymer Science, Japan
  • for the work of "Preparation of Temperature Responsive Nanogels with Carboxylic Acids which Undergo Large and Reversible pKa Shift"